FastQCFastQC Report
Thu 26 May 2016
SRR1364512_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364512_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4151470
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77150.050.0742871
GGTATCA45050.047.0393331
ATCAACG88700.041.7238433
TCAACGC90900.040.4281544
CAACGCA91850.040.268425
AACGCAG92350.039.065096
TATCAAC103750.037.277162
GTACATG134950.034.1408271
TACATGG140100.032.3619272
ACGCAGA109550.032.2259647
CGCAGAG112650.030.2832668
ACATGGG146250.027.9904633
GCAGAGT125650.025.5390979
CATGGGG80650.021.2691784
GAGTACA103350.021.2530781
CATGGGA81900.020.944564
GAGTACT61150.020.76831412-13
CCAATAC29150.020.6158073
AGAGTAC126100.020.55221610-11
AGTACAT101900.020.0279942
Produced by FastQC (version 0.11.5)