##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364512_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4151470 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.210654298356967 30.0 25.0 31.0 2.0 34.0 2 25.510834957256105 30.0 25.0 31.0 2.0 34.0 3 25.446656726412574 30.0 25.0 31.0 2.0 34.0 4 28.127499174991026 35.0 26.0 37.0 2.0 37.0 5 28.749064307341737 35.0 27.0 37.0 2.0 37.0 6 28.85943894572284 35.0 28.0 37.0 2.0 37.0 7 28.629959026561675 35.0 27.0 37.0 2.0 37.0 8 28.267134533069008 35.0 26.0 37.0 2.0 37.0 9 28.828183510900956 35.0 25.0 39.0 2.0 39.0 10-11 28.882889675223474 35.0 26.0 39.0 2.0 39.0 12-13 28.72436570660513 35.0 25.5 39.0 2.0 39.0 14-15 29.320304012795468 36.0 25.0 40.0 2.0 41.0 16-17 29.538686778418246 36.0 26.5 40.0 2.0 41.0 18-19 29.46264419591133 36.0 26.0 40.0 2.0 41.0 20-21 29.238598857754 35.5 24.5 40.0 2.0 41.0 22-23 29.26478994187601 36.0 25.0 40.0 2.0 41.0 24-25 29.26927510014525 36.0 25.0 40.0 2.0 41.0 26-27 29.360465810905534 36.0 25.5 40.0 2.0 41.0 28-29 29.271320881519074 36.0 25.0 40.0 2.0 41.0 30-31 28.927063305287042 35.0 24.0 40.0 2.0 41.0 32-33 28.929186288230433 35.0 24.5 40.0 2.0 41.0 34-35 28.64020479492806 35.0 24.0 40.0 2.0 41.0 36-37 28.405629331297106 35.0 23.0 40.0 2.0 41.0 38-39 28.25535099615317 35.0 22.5 39.0 2.0 41.0 40-41 28.29104196826666 35.0 23.0 39.5 2.0 41.0 42-43 28.14663312031642 35.0 22.0 39.0 2.0 41.0 44-45 27.811094744753063 34.0 20.0 39.0 2.0 41.0 46-47 27.5791519630396 34.0 19.0 39.0 2.0 40.5 48-49 27.347490768330253 33.5 17.5 39.0 2.0 40.0 50-51 27.338125531438262 33.5 18.5 39.0 2.0 40.0 52-53 27.14475342469053 33.0 16.5 39.0 2.0 40.0 54-55 27.015275071239827 33.0 16.0 39.0 2.0 40.0 56-57 26.60441397866298 33.0 13.0 38.5 2.0 40.0 58-59 26.38746468118522 33.0 12.5 38.0 2.0 40.0 60-61 25.9832749604357 32.5 8.5 38.0 2.0 40.0 62-63 25.92910631655775 32.0 9.0 38.0 2.0 40.0 64-65 25.749380821733023 32.0 8.0 37.5 2.0 40.0 66-67 25.50549648678661 32.0 8.0 37.0 2.0 40.0 68-69 25.33956490110732 32.0 7.5 36.5 2.0 39.5 70-71 25.214439222733155 32.0 7.0 36.0 2.0 39.0 72-73 25.19142845787155 32.0 6.5 36.0 2.0 39.0 74-75 24.92499801275211 32.0 2.0 35.5 2.0 39.0 76-77 24.64934565346733 32.0 2.0 35.0 2.0 38.5 78-79 24.350175720889226 31.5 2.0 35.0 2.0 37.0 80-81 24.354012554589097 32.0 2.0 35.0 2.0 37.0 82-83 24.17203809734865 32.0 2.0 35.0 2.0 36.0 84-85 23.999746234466343 32.0 2.0 35.0 2.0 36.0 86-87 23.808466398649152 31.0 2.0 35.0 2.0 36.0 88-89 23.59542764370211 31.0 2.0 35.0 2.0 35.0 90-91 23.439354132391657 31.0 2.0 35.0 2.0 35.0 92-93 23.33762703331591 31.0 2.0 35.0 2.0 35.0 94-95 23.274514689977288 31.0 2.0 35.0 2.0 35.0 96-97 23.148869797927 31.0 2.0 35.0 2.0 35.0 98-99 23.06154843946843 31.0 2.0 35.0 2.0 35.0 100-101 23.00810411733675 31.0 2.0 35.0 2.0 35.0 102-103 21.935463101022048 29.5 2.0 33.5 2.0 34.5 104-105 22.671657388828535 30.5 2.0 34.0 2.0 35.0 106-107 22.766645067891616 31.0 2.0 34.0 2.0 35.0 108-109 22.71911274801456 31.0 2.0 35.0 2.0 35.0 110-111 22.61728520259089 31.0 2.0 34.5 2.0 35.0 112-113 22.601741913105478 31.0 2.0 34.5 2.0 35.0 114-115 22.56212498223521 31.0 2.0 35.0 2.0 35.0 116-117 22.37264390685709 31.0 2.0 35.0 2.0 35.0 118-119 22.2295838582478 31.0 2.0 34.5 2.0 35.0 120-121 22.08584019636418 31.0 2.0 34.0 2.0 35.0 122-123 21.819591012340208 31.0 2.0 34.0 2.0 35.0 124-125 20.878467747568934 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6468673430953373 1101 2 1.3307887537620928 1101 3 1.579135220138241 1101 4 1.8987594446119154 1101 5 1.4384658360484117 1101 6 1.3855275493806225 1101 7 1.275103261641327 1101 8 1.6449654882972737 1101 9 2.1681868093832755 1101 10-11 2.169612479668551 1101 12-13 1.873550060925396 1101 14-15 2.2744478648001483 1101 16-17 1.8473944111602556 1101 18-19 1.8898672640104195 1101 20-21 2.246295413794556 1101 22-23 2.0766069040590125 1101 24-25 1.9949715279602316 1101 26-27 1.6655536145445922 1101 28-29 1.581314499287494 1101 30-31 1.7775033936509281 1101 32-33 1.9331616222938948 1101 34-35 2.1652168019941094 1101 36-37 2.1864660273838723 1101 38-39 2.244407853968898 1101 40-41 2.0352765529372476 1101 42-43 1.9649003819608346 1101 44-45 2.184366588467036 1101 46-47 2.115953405803495 1101 48-49 2.0658195245563604 1101 50-51 2.114406270461032 1101 52-53 1.976138071601028 1101 54-55 2.046715549565427 1101 56-57 2.2912135964084186 1101 58-59 2.252378822066362 1101 60-61 2.454660396969633 1101 62-63 2.1493814054636147 1101 64-65 1.9912475984574947 1101 66-67 2.0988752696645214 1101 68-69 1.9513676407986509 1101 70-71 1.9376408759188593 1101 72-73 1.8245167345783635 1101 74-75 1.8060423823644705 1101 76-77 1.9918996528274668 1101 78-79 1.9878333427803874 1101 80-81 1.802284613992569 1101 82-83 1.820091903428196 1101 84-85 1.7232530416164664 1101 86-87 1.6955998667470347 1101 88-89 1.8190414589733983 1101 90-91 1.788616595677194 1101 92-93 1.8055880399469437 1101 94-95 1.8129387256031038 1101 96-97 1.8177791196018234 1101 98-99 1.7015440484396223 1101 100-101 1.6680615746967788 1101 102-103 1.6898572475270015 1101 104-105 1.6822872214147004 1101 106-107 1.807135374022998 1101 108-109 1.8220507474434235 1101 110-111 1.9581341161388366 1101 112-113 1.8934686697886463 1101 114-115 1.8272971084584881 1101 116-117 1.9706699325288461 1101 118-119 2.0130793394458166 1101 120-121 2.0124440795842453 1101 122-123 1.9989061737963567 1101 124-125 2.112214543321702 1102 1 0.7466461823373791 1102 2 0.44929517038643496 1102 3 0.5771866298283577 1102 4 0.9331652863467497 1102 5 1.0069410664078227 1102 6 0.9728968819983876 1102 7 0.9628783704193609 1102 8 0.8566744016634971 1102 9 1.3514334865073572 1102 10-11 0.9970104762314485 1102 12-13 1.2714089076529085 1102 14-15 1.5595937451932933 1102 16-17 1.0684779464421545 1102 18-19 1.1553379954179661 1102 20-21 1.2437325173392821 1102 22-23 1.223101819058467 1102 24-25 0.9103448226620934 1102 26-27 0.9213688750874738 1102 28-29 0.7104647747102426 1102 30-31 0.6656336212809428 1102 32-33 0.6513524229880687 1102 34-35 1.0879483247792585 1102 36-37 1.267299618126124 1102 38-39 1.2290551376001666 1102 40-41 0.8696811052307538 1102 42-43 0.8828583594035706 1102 44-45 1.2640443316743628 1102 46-47 1.1398882170187434 1102 48-49 1.3121029855587665 1102 50-51 1.1942698801643061 1102 52-53 1.1792359376091852 1102 54-55 1.090452177004316 1102 56-57 1.230294372381742 1102 58-59 1.2692516171935964 1102 60-61 1.6661522144338257 1102 62-63 1.4750558887331806 1102 64-65 1.5319195018857208 1102 66-67 1.436617389835849 1102 68-69 1.2580475029079992 1102 70-71 1.4117364790293934 1102 72-73 1.2941332307607532 1102 74-75 1.4476179109347491 1102 76-77 1.3194545633747552 1102 78-79 1.2785536122419856 1102 80-81 0.8667747037012532 1102 82-83 0.8804748828410425 1102 84-85 0.9331826937242376 1102 86-87 1.040505264894879 1102 88-89 0.812477004259442 1102 90-91 0.8592940144629999 1102 92-93 0.8065782493963134 1102 94-95 0.9511523834423237 1102 96-97 1.085418629004387 1102 98-99 1.162317373857988 1102 100-101 1.0576636109211606 1102 102-103 0.8884505293555414 1102 104-105 0.8372905078123658 1102 106-107 1.0546850280596587 1102 108-109 1.2185852564404662 1102 110-111 1.0227986159688598 1102 112-113 0.8928624885616472 1102 114-115 0.9373196962109596 1102 116-117 1.1153924169534513 1102 118-119 1.2424516576235618 1102 120-121 1.2219424159652625 1102 122-123 1.4190368838404055 1102 124-125 1.3417003706838209 1103 1 1.2171560543139641 1103 2 0.9837066165704442 1103 3 1.1792337576161955 1103 4 1.7521824569162092 1103 5 1.085335280869085 1103 6 1.0083845024515874 1103 7 1.0459447247826112 1103 8 1.480103758343251 1103 9 2.0382509389906325 1103 10-11 1.609075366529769 1103 12-13 1.7562242984238594 1103 14-15 1.966113273207828 1103 16-17 1.8278948636106378 1103 18-19 1.4585998600835524 1103 20-21 1.8668835338201433 1103 22-23 1.744888938844067 1103 24-25 1.7849861414960415 1103 26-27 1.459933454721373 1103 28-29 1.436276258898694 1103 30-31 1.5761433895917598 1103 32-33 1.5928327781831157 1103 34-35 1.3689079268967426 1103 36-37 1.8173032749597269 1103 38-39 1.7923814500348918 1103 40-41 1.9014647788429926 1103 42-43 1.7922164866710801 1103 44-45 1.6857129824149908 1103 46-47 1.9475230055752988 1103 48-49 1.962995554920436 1103 50-51 1.8848726428474087 1103 52-53 1.9075997157070077 1103 54-55 1.8340693261178203 1103 56-57 2.061332123268347 1103 58-59 2.1342888973995926 1103 60-61 2.164522878696463 1103 62-63 1.9017389190565623 1103 64-65 1.8008232630603445 1103 66-67 1.8124337933927812 1103 68-69 1.6362470777642386 1103 70-71 1.868552602878978 1103 72-73 1.8891843698430222 1103 74-75 1.8261517365504822 1103 76-77 1.8447302263339616 1103 78-79 1.9899318618902626 1103 80-81 1.5060071214883592 1103 82-83 1.6211591230104574 1103 84-85 1.5917915861924996 1103 86-87 1.637929981437967 1103 88-89 1.6547222011820715 1103 90-91 1.6070592920623206 1103 92-93 1.5359146548819993 1103 94-95 1.3576276632947106 1103 96-97 1.5179237045848168 1103 98-99 1.4648558488356898 1103 100-101 1.3349596268936779 1103 102-103 1.3367749767575745 1103 104-105 1.5153974325807944 1103 106-107 1.2902250211226125 1103 108-109 1.641371690415216 1103 110-111 1.7587595015445885 1103 112-113 1.6440625862166982 1103 114-115 1.536448145320719 1103 116-117 1.6350810420895812 1103 118-119 1.7052657375456803 1103 120-121 1.688636710192096 1103 122-123 1.7032371466949385 1103 124-125 1.6302523962985838 1104 1 1.3363494598621486 1104 2 1.1472708112225902 1104 3 1.1869058665148273 1104 4 1.3856079034348454 1104 5 1.1596564212809213 1104 6 0.9742006474875424 1104 7 1.011520788707152 1104 8 1.3065687411387614 1104 9 1.7899116456594086 1104 10-11 1.6471963633242055 1104 12-13 1.1097492620473837 1104 14-15 1.4505675048547566 1104 16-17 1.1842821878727179 1104 18-19 0.5143736167416755 1104 20-21 -4.014620196981767 1104 22-23 -0.7258091921504715 1104 24-25 0.6947276170163512 1104 26-27 0.8380079093284536 1104 28-29 0.3920360878107658 1104 30-31 1.1932591867246067 1104 32-33 1.0444769649471084 1104 34-35 1.3155421767496094 1104 36-37 1.4284992125612455 1104 38-39 1.3477595393115642 1104 40-41 1.450416448111504 1104 42-43 1.4142013316672504 1104 44-45 1.5612364305108954 1104 46-47 1.531731392983815 1104 48-49 1.6245406690144613 1104 50-51 1.5242855674688869 1104 52-53 1.5080134305964208 1104 54-55 1.417789640906669 1104 56-57 1.7355557649181534 1104 58-59 1.6947751623176686 1104 60-61 1.8033512283965614 1104 62-63 1.520454352778735 1104 64-65 1.6742007124233638 1104 66-67 1.6851594124375104 1104 68-69 1.576047153322719 1104 70-71 1.761138833694961 1104 72-73 1.5747611900786787 1104 74-75 1.6442992151290845 1104 76-77 1.5982883614178434 1104 78-79 1.527203994794732 1104 80-81 1.3558964462977414 1104 82-83 1.272394686139016 1104 84-85 1.2819044004815332 1104 86-87 1.1564944457472563 1104 88-89 1.3147621835281562 1104 90-91 1.2923984485675142 1104 92-93 0.9508432511231817 1104 94-95 1.1824142006118983 1104 96-97 1.3075327412224134 1104 98-99 1.1903160727727808 1104 100-101 0.7388084242318982 1104 102-103 1.1674152304314092 1104 104-105 1.3397294682307397 1104 106-107 1.4230063888309061 1104 108-109 1.4792271225902702 1104 110-111 1.5232796307693768 1104 112-113 1.4979610635708553 1104 114-115 1.412297928496404 1104 116-117 1.5326327436261593 1104 118-119 1.4781602532096763 1104 120-121 1.5068349424215555 1104 122-123 1.3041664333310372 1104 124-125 1.4729912784798387 1105 1 1.208083473092323 1105 2 0.8773895614010208 1105 3 0.9279480997494041 1105 4 1.4792687279862804 1105 5 1.1381722581683036 1105 6 1.1329135371934065 1105 7 1.0695179955062457 1105 8 1.403414049565054 1105 9 1.7809272177774993 1105 10-11 1.5007245292512081 1105 12-13 1.602004737246098 1105 14-15 1.763061587836674 1105 16-17 1.5451045290594934 1105 18-19 1.1922115866841452 1105 20-21 1.3389599212849177 1105 22-23 1.2007452223133903 1105 24-25 1.2817797885389588 1105 26-27 1.2917034670062577 1105 28-29 0.8082589742841435 1105 30-31 1.2761514758139931 1105 32-33 1.1857137230290178 1105 34-35 1.113896773743118 1105 36-37 1.233595445838187 1105 38-39 1.084221033568145 1105 40-41 1.2476194713623912 1105 42-43 1.542096445729861 1105 44-45 1.6804030565028576 1105 46-47 1.9028570814990893 1105 48-49 1.4992397414470524 1105 50-51 1.6040297496817288 1105 52-53 1.4980058190548746 1105 54-55 1.6026093238097587 1105 56-57 2.0188300585367074 1105 58-59 1.6833055292833023 1105 60-61 2.0593293966719486 1105 62-63 1.8691793735228366 1105 64-65 1.570308834071728 1105 66-67 1.75655095234492 1105 68-69 1.3403613064615918 1105 70-71 1.4392515075332675 1105 72-73 1.3368577852837262 1105 74-75 1.6692686515434723 1105 76-77 1.6014415334460494 1105 78-79 1.4610088860735573 1105 80-81 1.409648952396644 1105 82-83 1.4686396014174434 1105 84-85 1.448164953264559 1105 86-87 1.486743508463963 1105 88-89 1.603371681976835 1105 90-91 1.6248108030249888 1105 92-93 1.6372049849153854 1105 94-95 1.4985070508279996 1105 96-97 1.6152032112040224 1105 98-99 1.4896423845779907 1105 100-101 1.5922655744302219 1105 102-103 1.5970930706691533 1105 104-105 1.6502665953319706 1105 106-107 1.7507955721534785 1105 108-109 1.8381202856485537 1105 110-111 1.7791605941796433 1105 112-113 1.767068231589306 1105 114-115 1.7098411687752275 1105 116-117 1.927261184821127 1105 118-119 2.0188373235443713 1105 120-121 1.9253155511791107 1105 122-123 1.9726125414987337 1105 124-125 1.706481808027629 1106 1 -0.46425462543901475 1106 2 -0.3053716855459534 1106 3 -0.31834919841542586 1106 4 -0.7553270180604734 1106 5 -1.1734904837027003 1106 6 -0.6132549576401622 1106 7 -0.5758117894616426 1106 8 -0.16169407928496682 1106 9 -0.11360403906171257 1106 10-11 -2.6699322393434244 1106 12-13 -0.7739550284917485 1106 14-15 -0.5182848651110099 1106 16-17 -0.7499896806267294 1106 18-19 -3.410752757495633 1106 20-21 -5.674974591529757 1106 22-23 -6.47126932131604 1106 24-25 -6.359273515730461 1106 26-27 -5.3772290177541535 1106 28-29 -2.1146311927166437 1106 30-31 -3.8939811501807036 1106 32-33 -4.505052122786331 1106 34-35 -3.8774079800744374 1106 36-37 -6.204608544322102 1106 38-39 -6.560990686542862 1106 40-41 -2.8787818265923377 1106 42-43 -1.4606181121980804 1106 44-45 -0.660844765892115 1106 46-47 -0.5737977086483603 1106 48-49 -0.3593287732293504 1106 50-51 -0.4020178789538136 1106 52-53 -0.6499903174288022 1106 54-55 -0.6005889907166555 1106 56-57 -0.4578697865141095 1106 58-59 -0.30877718031391765 1106 60-61 -0.09294146967198103 1106 62-63 -0.2091014784350378 1106 64-65 -0.3996541940613234 1106 66-67 -0.6501335728715283 1106 68-69 -0.5941151491415297 1106 70-71 -0.376684972056335 1106 72-73 -0.3348626175257827 1106 74-75 -0.3473749916006845 1106 76-77 -0.4109859161954681 1106 78-79 -0.4719379205434464 1106 80-81 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-8.314109930907215 2216 12-13 -3.8405151755092852 2216 14-15 -3.1100536414679603 2216 16-17 -3.073314988118554 2216 18-19 -2.6125254758736745 2216 20-21 -2.2088183456951143 2216 22-23 -2.4235329772995406 2216 24-25 -2.778894401068694 2216 26-27 -2.5764347714033633 2216 28-29 -3.0697829038090347 2216 30-31 -2.8337044713476516 2216 32-33 -3.128379699067274 2216 34-35 -2.7200407964074493 2216 36-37 -2.3979982003453095 2216 38-39 -2.651581223990643 2216 40-41 -2.8518771639470977 2216 42-43 -2.9791827060422875 2216 44-45 -2.84671763996581 2216 46-47 -2.993228129140064 2216 48-49 -2.8553667867910377 2216 50-51 -2.7801392081239165 2216 52-53 -3.220986741249952 2216 54-55 -3.787046077988464 2216 56-57 -2.848402980316912 2216 58-59 -3.760497064265355 2216 60-61 -3.2754677663558596 2216 62-63 -3.4838047633580835 2216 64-65 -3.285662061087592 2216 66-67 -4.084336759953043 2216 68-69 -3.652191669726534 2216 70-71 -5.64058842373538 2216 72-73 -6.037992809153383 2216 74-75 -3.9852503840303015 2216 76-77 -3.4236993596217893 2216 78-79 -3.784047355586594 2216 80-81 -3.7401922698397954 2216 82-83 -3.996046566567344 2216 84-85 -3.7025416120113377 2216 86-87 -3.994015208528271 2216 88-89 -4.153244866200783 2216 90-91 -4.197762529864871 2216 92-93 -3.9414546973712383 2216 94-95 -7.121731716201925 2216 96-97 -9.003600833978723 2216 98-99 -5.822961632755927 2216 100-101 -4.852654749179887 2216 102-103 -5.906004801037962 2216 104-105 -8.597519620401714 2216 106-107 -5.199958742018474 2216 108-109 -7.545129773685225 2216 110-111 -5.015353581943316 2216 112-113 -4.38183954764731 2216 114-115 -4.828018237503613 2216 116-117 -4.340211669621411 2216 118-119 -4.643940297951765 2216 120-121 -5.380759438828573 2216 122-123 -8.406376140265305 2216 124-125 -6.935314204403994 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 505759.0 3 71375.0 4 40343.0 5 32356.0 6 31807.0 7 34959.0 8 38514.0 9 40666.0 10 41849.0 11 42565.0 12 42465.0 13 42179.0 14 41239.0 15 35485.0 16 29954.0 17 26922.0 18 24975.0 19 23866.0 20 23988.0 21 26689.0 22 33097.0 23 44364.0 24 58949.0 25 75167.0 26 90251.0 27 103434.0 28 116450.0 29 130270.0 30 145845.0 31 163432.0 32 190941.0 33 228664.0 34 277622.0 35 342408.0 36 430075.0 37 415535.0 38 106770.0 39 241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.637829585358254 18.610222486693136 13.435904755750716 24.31604317219789 2 15.126952894396839 19.73325121526548 40.73395914626124 24.40583674407644 3 18.853457106937395 25.091554215494178 28.698417992316124 27.356570685252308 4 11.58391192964783 16.251539375477478 37.906666248936595 34.2578824459381 5 13.714916185418282 37.883699284550225 34.375715485923095 14.025669044108396 6 32.13869320683778 36.85197283159074 17.34024009874516 13.66909386282632 7 28.80202825586857 32.06968817372662 20.840449492333818 18.28783407807099 8 25.83884218784891 35.800885832959764 19.883666674937075 18.476605304254257 9 26.046682832421382 16.406225533958928 20.317877259427604 37.2292143741921 10-11 24.71528482604482 26.771613973681667 27.522354337979227 20.99074686229429 12-13 25.49849964401701 23.854449630785837 28.028116814453906 22.61893391074324 14-15 22.557631921067248 25.453071102667046 25.480414066425094 26.508882909840615 16-17 22.782065660623743 28.759524259833253 25.70241303334349 22.75599704619951 18-19 22.673487260475127 27.41683561549776 27.807519999807262 22.102157124219854 20-21 23.279403932873706 26.79407695558296 27.76794897027865 22.158570141264686 22-23 23.062753341243504 26.646963518860932 27.424854394661235 22.865428745234322 24-25 22.812299098648957 26.811540695107684 27.52594093053079 22.850219275712572 26-27 22.649010403473824 27.115760251678957 27.280810443053106 22.954418901794114 28-29 22.641969851449343 27.218356277073745 27.320240576545412 22.8194332949315 30-31 22.728638101833727 26.883189304748427 27.452892114058596 22.93528047935925 32-33 22.62664396958514 27.053695026816982 27.09420144009152 23.22545956350636 34-35 23.01354387665763 26.87161890958012 27.358047808323406 22.756789405438848 36-37 23.04799733002228 26.54838051903895 27.3014134814845 23.102208669454278 38-39 22.728800256703785 26.882867816822966 27.495068762695468 22.893263163777785 40-41 22.952200039589233 26.80707791837751 27.297834989531022 22.942887052502236 42-43 22.78464651818302 26.764012190274283 27.189563583362443 23.261777708180254 44-45 22.894758989527723 26.868555504620012 27.208283004003274 23.028402501848994 46-47 23.004394176797355 27.10138620782771 26.87648018766217 23.017739427712762 48-49 23.002923237345936 27.053032504920143 26.81159952720379 23.13244473053013 50-51 23.037386442205072 27.085657087379577 26.674716993859267 23.202239476556084 52-53 23.243274887049516 26.91320796717076 26.722238898522598 23.121278247257123 54-55 23.03336092100006 26.855648005623696 26.814368890266334 23.296622183109918 56-57 22.839366765181264 27.1539543812015 26.850545323278794 23.156133530338437 58-59 22.989652448409803 26.827504910455406 26.81906083038192 23.36378181075287 60-61 23.19446574590541 26.75060158471625 26.975211685652845 23.079720983725498 62-63 23.014007111056078 26.9962129693724 26.778188601858066 23.211591317713452 64-65 22.949094102503167 26.798271663615914 26.7688581949212 23.483776038959718 66-67 22.780316406700276 27.20597029954724 26.63755216562238 23.3761611281301 68-69 22.945397044730857 26.873987917402015 26.859582076435757 23.321032961431374 70-71 22.658504308881312 26.774227001182656 27.21026418177747 23.357004508158564 72-73 23.037232373683512 26.809609465659705 26.781691357972647 23.37146680268414 74-75 22.974707807775147 26.964740614625914 26.73303673598874 23.3275148416102 76-77 23.07037151866121 26.765009345722348 26.936132786104494 23.228486349511947 78-79 22.936703877381728 26.845527546393726 26.882005017700244 23.3357635585243 80-81 22.8653343927545 27.014830330616245 26.819519124443087 23.300316152186166 82-83 23.093066464896552 27.061729947468947 26.608831200365962 23.236372387268535 84-85 23.053753598227082 26.907596324087347 26.589735869054643 23.448914208630928 86-87 23.017202390810134 26.805775281913853 26.809581307116726 23.367441020159287 88-89 22.985192772431017 26.85775685967678 26.751426569949626 23.405623797942578 90-91 22.940232105964213 27.0002496838001 26.84678990101898 23.212728309216704 92-93 23.038108848183082 26.91064856886313 26.81240300554099 23.238839577412797 94-95 22.938920761130984 26.687256220707944 26.900286079335224 23.473536938825852 96-97 23.15017724724247 26.657339702743975 26.711008286472687 23.48147476354087 98-99 22.72881111371536 26.74953792017467 26.723389730397827 23.79826123571214 100-101 22.760900966808194 26.66548311322572 26.910211291991104 23.663404627974987 102-103 22.879894012616113 26.515345588124596 27.032287009017956 23.572473390241335 104-105 22.893997502944266 26.48420767621816 27.02100480115935 23.600790019678225 106-107 22.679615107000835 26.63949456568017 27.108283765185547 23.572606562133448 108-109 22.768482628287174 26.522609079282176 26.81820683701246 23.89070145541819 110-111 22.64693182796774 26.556927859400716 27.115634648540933 23.68050566409061 112-113 22.938595778072063 26.59117228869734 26.993226824593002 23.47700510863759 114-115 22.735607331271844 26.722571143432994 26.897019704593696 23.64480182070147 116-117 22.478687245108123 26.682027427751027 27.096695709987547 23.742589617153303 118-119 22.665734272809274 26.65186254545691 26.786675038298046 23.895728143435772 120-121 22.61355365203567 26.778049760810042 26.91378164643295 23.694614940721337 122-123 22.73752364324167 26.64896856207658 26.95044097114826 23.663066823533494 124-125 22.752013947365757 26.66391360113985 26.673505548146032 23.91056690334836 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 429.0 1 466.0 2 601.0 3 650.0 4 549.0 5 509.0 6 551.5 7 659.0 8 845.5 9 1039.0 10 1115.0 11 1188.0 12 1345.5 13 1497.0 14 1676.5 15 1917.5 16 2138.0 17 2370.0 18 2684.0 19 3068.5 20 3662.0 21 4474.5 22 5678.0 23 7520.5 24 10034.5 25 13572.0 26 18818.0 27 25190.5 28 30923.5 29 37141.5 30 44956.5 31 53406.5 32 61865.0 33 69518.5 34 77287.5 35 84162.0 36 92845.5 37 102805.0 38 108987.0 39 113431.5 40 118184.5 41 124513.5 42 130516.0 43 136615.0 44 143371.0 45 148534.5 46 151720.0 47 153948.5 48 158859.0 49 162157.5 50 160038.0 51 156804.5 52 154369.0 53 151532.0 54 145643.0 55 135568.0 56 125034.5 57 115295.0 58 105708.0 59 94843.5 60 81162.0 61 67046.0 62 54011.5 63 41738.0 64 31491.5 65 24216.5 66 18702.5 67 14604.5 68 11550.0 69 9182.5 70 7281.5 71 5760.5 72 4601.0 73 3801.0 74 3085.0 75 2369.5 76 1703.0 77 1186.5 78 876.5 79 641.0 80 466.5 81 360.0 82 246.5 83 173.0 84 134.0 85 91.0 86 71.0 87 60.5 88 49.0 89 39.0 90 28.0 91 25.0 92 19.5 93 14.5 94 14.0 95 10.0 96 6.5 97 7.5 98 6.5 99 4.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09601418292797491 2 0.03247042613821128 3 0.04198512815942305 4 0.04962097762961072 5 0.052800574254420723 6 0.004287637872849858 7 0.0028905423861909155 8 0.0029628059458456884 9 0.00741905879122335 10-11 0.060195545192425814 12-13 0.006949345653467326 14-15 0.01188735556321014 16-17 0.021317750098158002 18-19 0.019390721840697393 20-21 0.13233866558110743 22-23 0.016741057986689054 24-25 0.0155487092523853 26-27 0.007431102717832479 28-29 0.004636911744514594 30-31 0.002288346055734475 32-33 0.006636203561629977 34-35 0.019559336813225196 36-37 0.0029989377256730747 38-39 0.009201559929374414 40-41 0.03255473362447518 42-43 0.014934468995319729 44-45 0.11121361830869547 46-47 0.004347857505895503 48-49 0.01015303013149559 50-51 0.012043926609128813 52-53 0.012911089324986089 54-55 0.011092456407007638 56-57 0.007431102717832479 58-59 0.01524761108715708 60-61 0.033470072046768974 62-63 0.00791285978219763 64-65 0.007479278424268994 66-67 0.009358130975293089 68-69 0.009057032810064869 70-71 0.005263195928189292 72-73 0.0010237337617759494 74-75 0.0012164365875220102 76-77 0.008984769250410096 78-79 0.023196602649182097 80-81 0.0035288704964747423 82-83 0.005082537029052359 84-85 0.0028905423861909155 86-87 0.0037938368818755766 88-89 0.006118314717437437 90-91 0.00528728378140755 92-93 0.003312079817510424 94-95 0.004191286459976827 96-97 0.0015777543857958749 98-99 0.0031555087715917497 100-101 0.0031193769917643626 102-103 7.82855229593373E-4 104-105 0.004359901432504631 106-107 7.346795231568577E-4 108-109 0.0012405244407402679 110-111 0.002348565688780119 112-113 0.004034715414058153 114-115 0.0024810488814805357 116-117 0.004817570643651526 118-119 0.0035770462029112576 120-121 0.0065398521487569465 122-123 0.004287637872849858 124-125 0.05193341153856345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4151470.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.20134974237607 #Duplication Level Percentage of deduplicated Percentage of total 1 80.82336057367851 48.656753972501896 2 9.731259799603341 11.716699492596904 3 3.7686054553557433 6.806254051766924 4 1.8512457816076446 4.457899790306406 5 1.0427567298933682 3.138768129626351 6 0.6436662822071142 2.324974738351525 7 0.43131368478105747 1.8175966190324169 8 0.30529223394606436 1.4703203639534652 9 0.2332144375101825 1.2635841525769804 >10 1.0696102091167823 11.449440816119365 >50 0.06597069133784285 2.736578380265929 >100 0.03208498996641951 3.3727158781329654 >500 0.0013700339602893351 0.5103881870171036 >1k 2.490970356737731E-4 0.27802542775171507 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.408785321825763E-5 0.0 2 0.0 0.0 0.0 2.408785321825763E-5 0.0 3 0.0 0.0 0.0 2.408785321825763E-5 0.0 4 0.0 0.0 0.0 2.408785321825763E-5 0.0 5 0.0 0.0 0.0 2.408785321825763E-5 2.408785321825763E-5 6 0.0 0.0 0.0 2.408785321825763E-5 2.408785321825763E-5 7 4.817570643651526E-5 0.0 0.0 2.408785321825763E-5 2.408785321825763E-5 8 4.817570643651526E-5 0.0 0.0 4.817570643651526E-5 2.408785321825763E-5 9 4.817570643651526E-5 0.0 0.0 4.817570643651526E-5 2.408785321825763E-5 10-11 4.817570643651526E-5 0.0 0.0 4.817570643651526E-5 2.408785321825763E-5 12-13 6.021963304564408E-5 0.0 0.0 6.021963304564408E-5 4.817570643651526E-5 14-15 9.635141287303052E-5 0.0 0.0 8.430748626390171E-5 4.817570643651526E-5 16-17 9.635141287303052E-5 0.0 0.0 9.635141287303052E-5 4.817570643651526E-5 18-19 9.635141287303052E-5 0.0 0.0 9.635141287303052E-5 4.817570643651526E-5 20-21 9.635141287303052E-5 0.0 0.0 1.3248319270041696E-4 4.817570643651526E-5 22-23 1.4452711930954578E-4 0.0 0.0 1.565710459186746E-4 4.817570643651526E-5 24-25 1.9270282574606105E-4 0.0 0.0 2.2883460557344748E-4 4.817570643651526E-5 26-27 1.9270282574606105E-4 0.0 0.0 3.010981652282204E-4 4.817570643651526E-5 28-29 1.9270282574606105E-4 0.0 0.0 9.394262755120476E-4 4.817570643651526E-5 30-31 1.9270282574606105E-4 1.2043926609128815E-5 0.0 0.0032639041110739085 6.021963304564408E-5 32-33 1.9270282574606105E-4 2.408785321825763E-5 0.0 0.007756288736278956 7.226355965477289E-5 34-35 1.9270282574606105E-4 2.408785321825763E-5 0.0 0.0135012417288334 7.226355965477289E-5 36-37 2.1679067896431868E-4 2.408785321825763E-5 0.0 0.023919238245729828 8.430748626390171E-5 38-39 2.649663854008339E-4 2.408785321825763E-5 0.0 0.04143110753540312 9.635141287303052E-5 40-41 2.649663854008339E-4 2.408785321825763E-5 0.0 0.062243012715977715 9.635141287303052E-5 42-43 2.8905423861909157E-4 4.817570643651526E-5 0.0 0.08285017114419713 9.635141287303052E-5 44-45 3.1314209183734917E-4 4.817570643651526E-5 0.0 0.10793767027101243 9.635141287303052E-5 46-47 3.3722994505560683E-4 4.817570643651526E-5 0.0 0.13844493637193572 1.0839533948215932E-4 48-49 3.492738716647356E-4 4.817570643651526E-5 0.0 0.17319166463927235 1.2043926609128814E-4 50-51 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.21456255254162981 1.2043926609128814E-4 52-53 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.2537053140212985 1.4452711930954578E-4 54-55 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.29853280886047595 1.4452711930954578E-4 56-57 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.3511527242157597 1.565710459186746E-4 58-59 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.41093877590347516 1.6861497252780342E-4 60-61 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.48011909034631106 1.6861497252780342E-4 62-63 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.551828629377064 1.6861497252780342E-4 64-65 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.6266575453995813 1.6861497252780342E-4 66-67 3.854056514921221E-4 4.817570643651526E-5 0.0 0.7122778196638782 1.6861497252780342E-4 68-69 3.854056514921221E-4 4.817570643651526E-5 0.0 0.8141694387771079 1.6861497252780342E-4 70-71 4.094935047103797E-4 4.817570643651526E-5 0.0 0.9340185524645487 1.6861497252780342E-4 72-73 4.094935047103797E-4 4.817570643651526E-5 0.0 1.0598534976767264 1.6861497252780342E-4 74-75 4.094935047103797E-4 4.817570643651526E-5 0.0 1.1872180215682637 1.6861497252780342E-4 76-77 4.094935047103797E-4 4.817570643651526E-5 0.0 1.3294929266019024 1.6861497252780342E-4 78-79 4.094935047103797E-4 4.817570643651526E-5 0.0 1.5007093872772779 1.6861497252780342E-4 80-81 4.8175706436515256E-4 4.817570643651526E-5 0.0 1.6883176320676774 1.6861497252780342E-4 82-83 5.058449175834102E-4 4.817570643651526E-5 0.0 1.8883913408985251 1.6861497252780342E-4 84-85 5.17888844192539E-4 4.817570643651526E-5 0.0 2.0904041219134424 1.8065889913693222E-4 86-87 5.299327708016678E-4 4.817570643651526E-5 0.0 2.3069539223455786 1.9270282574606105E-4 88-89 5.299327708016678E-4 6.021963304564408E-5 0.0 2.5480612891337286 1.9270282574606105E-4 90-91 5.540206240199255E-4 7.226355965477289E-5 0.0 2.820482865105613 1.9270282574606105E-4 92-93 5.540206240199255E-4 7.226355965477289E-5 0.0 3.1131382377808343 1.9270282574606105E-4 94-95 5.540206240199255E-4 7.226355965477289E-5 0.0 3.411598783081655 1.9270282574606105E-4 96-97 5.540206240199255E-4 7.226355965477289E-5 0.0 3.7154188757235387 1.9270282574606105E-4 98-99 5.781084772381831E-4 7.226355965477289E-5 0.0 4.041773155051103 1.9270282574606105E-4 100-101 5.90152403847312E-4 7.226355965477289E-5 0.0 4.414978308888177 1.9270282574606105E-4 102-103 6.021963304564407E-4 7.226355965477289E-5 0.0 4.826471105415672 1.9270282574606105E-4 104-105 7.346795231568577E-4 7.226355965477289E-5 0.0 5.234158021134682 2.1679067896431868E-4 106-107 7.467234497659865E-4 7.226355965477289E-5 0.0 5.647108132781883 2.1679067896431868E-4 108-109 7.467234497659865E-4 7.226355965477289E-5 0.0 6.087012552180312 2.1679067896431868E-4 110-111 7.587673763751154E-4 7.226355965477289E-5 0.0 6.5711543140140725 2.1679067896431868E-4 112-113 7.708113029842442E-4 7.226355965477289E-5 0.0 7.098642167714087 3.1314209183734917E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7715 0.0 50.074287 1 GGTATCA 4505 0.0 47.039333 1 ATCAACG 8870 0.0 41.723843 3 TCAACGC 9090 0.0 40.428154 4 CAACGCA 9185 0.0 40.26842 5 AACGCAG 9235 0.0 39.06509 6 TATCAAC 10375 0.0 37.27716 2 GTACATG 13495 0.0 34.140827 1 TACATGG 14010 0.0 32.361927 2 ACGCAGA 10955 0.0 32.225964 7 CGCAGAG 11265 0.0 30.283266 8 ACATGGG 14625 0.0 27.990463 3 GCAGAGT 12565 0.0 25.539097 9 CATGGGG 8065 0.0 21.269178 4 GAGTACA 10335 0.0 21.253078 1 CATGGGA 8190 0.0 20.94456 4 GAGTACT 6115 0.0 20.768314 12-13 CCAATAC 2915 0.0 20.615807 3 AGAGTAC 12610 0.0 20.552216 10-11 AGTACAT 10190 0.0 20.027994 2 >>END_MODULE