FastQCFastQC Report
Thu 26 May 2016
SRR1364512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4151470
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53770.12952038675457128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51750.12465464040448324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56750.064.982711
GTATCAA99900.051.7879681
ATCAACG124800.039.0173873
TATCAAC133150.038.2267532
TCAACGC126250.038.19164
CAACGCA126950.037.6307335
AACGCAG129500.036.38446
GTACATG137700.034.4515271
TACATGG143550.032.094232
ACGCAGA156650.029.964467
CGCAGAG160500.029.3829788
ACATGGG145950.028.9937883
GCAGAGT179100.025.5677689
CATGGGG76550.024.0582494
GAGTACT106450.023.57739412-13
GTGGTAT30250.022.4882011
AGAGTAC174200.022.14067610-11
GTACTTT118300.022.0451914-15
CAGAGTA184700.021.88021710-11
GAGTACA109400.021.7636031
Produced by FastQC (version 0.11.5)