##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364512_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4151470 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.89028585055414 31.0 28.0 31.0 25.0 34.0 2 29.12579736816116 31.0 28.0 33.0 25.0 34.0 3 29.204702189826737 31.0 28.0 34.0 25.0 34.0 4 32.61626219146471 35.0 32.0 37.0 26.0 37.0 5 31.72629984077929 35.0 32.0 37.0 20.0 37.0 6 31.758630316490304 35.0 32.0 37.0 20.0 37.0 7 31.197070676170128 35.0 31.0 37.0 17.0 37.0 8 31.321451919440584 35.0 32.0 37.0 17.0 37.0 9 31.938138057121936 37.0 30.0 39.0 11.0 39.0 10-11 32.11601902458647 37.0 31.0 39.0 15.5 39.0 12-13 31.857909487482747 37.0 30.5 39.0 12.5 39.0 14-15 32.81203236443959 38.0 31.0 40.0 12.0 41.0 16-17 32.85452984123696 38.0 31.0 40.0 12.0 41.0 18-19 32.694093537951616 38.0 31.0 40.0 10.0 41.0 20-21 32.56651150074552 38.0 30.5 40.0 10.0 41.0 22-23 32.53530195328402 38.0 31.0 40.0 10.0 41.0 24-25 32.33641613693463 37.0 30.0 40.0 10.0 41.0 26-27 32.41684355180213 37.0 30.0 40.0 10.0 41.0 28-29 32.302746015266884 37.0 30.0 40.0 9.0 41.0 30-31 31.80816638443732 37.0 30.0 40.0 8.0 41.0 32-33 31.76692737753133 37.0 30.0 40.0 8.0 41.0 34-35 31.579237113600726 37.0 30.0 40.0 4.0 41.0 36-37 31.56736252459972 37.0 30.0 40.0 2.0 41.0 38-39 31.333477057524203 37.0 30.0 40.0 2.0 41.0 40-41 31.05278335143937 36.0 29.5 40.0 2.0 41.0 42-43 30.947386347486553 36.0 29.0 40.0 2.0 41.0 44-45 30.88122966081894 36.0 29.0 40.0 2.0 41.0 46-47 30.76252833333735 36.0 28.5 40.0 2.0 41.0 48-49 30.51322471317389 36.0 28.0 40.0 2.0 41.0 50-51 30.176262143289 36.0 27.5 40.0 2.0 41.0 52-53 30.08067383360593 35.5 27.5 40.0 2.0 41.0 54-55 29.603394580714784 35.0 26.0 39.5 2.0 41.0 56-57 29.619479967336872 35.0 27.0 39.0 2.0 41.0 58-59 29.37742763406697 35.0 26.0 39.0 2.0 41.0 60-61 29.09155383514755 35.0 26.0 39.0 2.0 41.0 62-63 28.817845124738948 34.0 26.0 39.0 2.0 40.5 64-65 28.334471042787253 34.0 25.0 38.0 2.0 40.0 66-67 27.948457654758435 34.0 23.0 38.0 2.0 40.0 68-69 27.762335148754538 34.0 23.0 37.0 2.0 40.0 70-71 27.83884828747408 34.0 25.0 37.0 2.0 40.0 72-73 27.614981319869827 34.0 24.5 36.5 2.0 39.0 74-75 27.24681389965482 34.0 23.5 36.0 2.0 39.0 76-77 27.16177342001749 33.5 24.0 36.0 2.0 39.0 78-79 26.780006840950314 33.0 23.0 35.0 2.0 37.5 80-81 26.557804705321246 33.0 23.0 35.0 2.0 37.0 82-83 26.42419215362269 33.0 23.0 35.0 2.0 36.5 84-85 26.002798526786897 33.0 20.5 35.0 2.0 36.0 86-87 25.877356574899977 33.0 20.0 35.0 2.0 36.0 88-89 25.844426432083093 33.0 20.5 35.0 2.0 35.5 90-91 25.691023661498214 33.0 20.0 35.0 2.0 35.0 92-93 25.712384890171435 33.0 20.5 35.0 2.0 35.0 94-95 25.73261952994963 33.0 21.5 35.0 2.0 35.0 96-97 25.619478762944212 33.0 20.0 35.0 2.0 35.0 98-99 25.485485141407743 33.0 20.0 35.0 2.0 35.0 100-101 25.393954671477815 33.0 19.5 35.0 2.0 35.0 102-103 24.263670458897693 30.5 18.5 34.0 2.0 35.0 104-105 25.215533172587058 32.5 19.5 35.0 2.0 35.0 106-107 25.31747188345333 33.0 19.0 35.0 2.0 35.0 108-109 25.394969251855365 33.0 19.0 35.0 2.0 35.0 110-111 25.328654187552843 33.0 19.0 35.0 2.0 35.0 112-113 25.278658764244955 33.0 19.0 35.0 2.0 35.0 114-115 25.227219996772227 33.0 18.0 35.0 2.0 35.0 116-117 25.152047949280615 33.0 18.0 35.0 2.0 35.0 118-119 25.0291516017218 33.0 15.0 35.0 2.0 35.0 120-121 24.865635545963237 33.0 4.5 35.0 2.0 35.0 122-123 24.57595478228194 33.0 2.0 35.0 2.0 35.0 124-125 23.7940275372338 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9783719246096254 1101 2 0.9792114576493383 1101 3 0.9564315999132305 1101 4 1.0183533644855345 1101 5 1.2802028762664825 1101 6 1.2573829553165758 1101 7 1.7766645940570704 1101 8 1.571415015264634 1101 9 2.149359805902698 1101 10-11 1.8537766799119098 1101 12-13 2.1017283260927435 1101 14-15 2.0340654388987787 1101 16-17 2.0344104983700504 1101 18-19 2.0350377118447085 1101 20-21 2.265033557943994 1101 22-23 2.187512035056862 1101 24-25 2.3486448691407134 1101 26-27 2.0068566726081087 1101 28-29 2.0888418064775607 1101 30-31 2.559151610946067 1101 32-33 2.4113183306894435 1101 34-35 2.542910345264623 1101 36-37 2.3236502868180935 1101 38-39 2.500767916879962 1101 40-41 2.6725956704303258 1101 42-43 2.5976702105468803 1101 44-45 2.450810636091287 1101 46-47 2.169188009239683 1101 48-49 2.2741011815743093 1101 50-51 2.580378654872 1101 52-53 2.432464093640636 1101 54-55 2.7587826507707334 1101 56-57 2.524494741678499 1101 58-59 2.572441346479007 1101 60-61 2.5602238761437164 1101 62-63 2.5839435033152967 1101 64-65 2.7626862473035416 1101 66-67 2.8740422620205166 1101 68-69 2.786451539719703 1101 70-71 2.4477854103612984 1101 72-73 2.651008595954586 1101 74-75 2.715575278023202 1101 76-77 2.4475609499276665 1101 78-79 2.4589613020808123 1101 80-81 2.5029860992278543 1101 82-83 2.406175025927091 1101 84-85 2.662219943282235 1101 86-87 2.56710367291177 1101 88-89 2.4192574175099537 1101 90-91 2.412941677496768 1101 92-93 2.3330995415491635 1101 94-95 2.224150243979107 1101 96-97 2.158330690426773 1101 98-99 2.2836049496898383 1101 100-101 2.379704625062921 1101 102-103 2.178352968112822 1101 104-105 2.2914973350938332 1101 106-107 2.3559079855872227 1101 108-109 2.2984325306309437 1101 110-111 2.2694970020797136 1101 112-113 2.2271898983010843 1101 114-115 2.282518106549958 1101 116-117 2.2900767318419923 1101 118-119 2.148996398826373 1101 120-121 2.148603368887862 1101 122-123 2.1742147892696835 1101 124-125 1.916223566604085 1102 1 0.5352901655930751 1102 2 -1.7089675003171756 1102 3 -0.06398624810218934 1102 4 0.4823615099199472 1102 5 1.062232263257446 1102 6 0.5961316066759927 1102 7 1.3931895372209944 1102 8 1.1143809738218948 1102 9 1.9297995849662684 1102 10-11 1.620675863048433 1102 12-13 1.712711116221353 1102 14-15 1.6320907577742574 1102 16-17 1.644132991422893 1102 18-19 1.7019539021850392 1102 20-21 1.90656484285838 1102 22-23 1.8046804046488987 1102 24-25 2.00240187933948 1102 26-27 1.7741645942645263 1102 28-29 1.7198795555674167 1102 30-31 2.0120213800565026 1102 32-33 1.735883463007216 1102 34-35 2.0021762466646393 1102 36-37 1.4813879797477512 1102 38-39 1.4100929648097882 1102 40-41 1.5410071525362312 1102 42-43 1.4176643452665587 1102 44-45 1.3167881419071996 1102 46-47 1.2374775169070205 1102 48-49 1.3139086799680229 1102 50-51 1.4392632747034675 1102 52-53 1.2495326825239808 1102 54-55 1.5020403346426683 1102 56-57 1.2088736106016569 1102 58-59 1.2476107761931416 1102 60-61 1.1765290390667857 1102 62-63 1.3825591801991308 1102 64-65 1.5219440525459405 1102 66-67 1.6609813526470525 1102 68-69 1.593112994934291 1102 70-71 0.998297165176858 1102 72-73 1.3410499338137143 1102 74-75 1.5212001710091592 1102 76-77 1.405674191445108 1102 78-79 1.2426974252474814 1102 80-81 1.346890934048023 1102 82-83 0.9773036772750032 1102 84-85 1.0637118508635162 1102 86-87 1.187901460326323 1102 88-89 1.3010969929856024 1102 90-91 1.1065841038169424 1102 92-93 1.1252588562911505 1102 94-95 1.193874712897049 1102 96-97 1.2913081619214886 1102 98-99 1.0192810061736957 1102 100-101 0.9481788967298961 1102 102-103 0.6578347449993984 1102 104-105 1.0076330107812197 1102 106-107 1.2331504630632217 1102 108-109 1.0983521490636825 1102 110-111 1.0292958214308037 1102 112-113 1.0544004225582064 1102 114-115 1.2835488686893513 1102 116-117 0.874719974584643 1102 118-119 0.4389112468055565 1102 120-121 0.809034196640166 1102 122-123 1.1810983207935024 1102 124-125 1.1417872154411803 1103 1 0.7912493539985626 1103 2 0.754454985890284 1103 3 0.6296210266095166 1103 4 0.7398478424449095 1103 5 1.0168192403076581 1103 6 1.080737692465025 1103 7 1.4221366325871223 1103 8 1.1957047922865982 1103 9 1.9121735994401021 1103 10-11 1.6014073339106005 1103 12-13 1.6376098827984826 1103 14-15 1.4269279483141233 1103 16-17 1.4988926432999392 1103 18-19 1.6074953342048843 1103 20-21 1.810253760541194 1103 22-23 1.6038982702354971 1103 24-25 1.988365106905512 1103 26-27 1.5579113550660466 1103 28-29 1.6240388566547423 1103 30-31 2.1825988773005562 1103 32-33 1.9285291426509055 1103 34-35 2.0662472644655736 1103 36-37 1.8836567715689227 1103 38-39 1.9271432962501613 1103 40-41 2.063190972193567 1103 42-43 2.017489435443892 1103 44-45 1.8963472766157246 1103 46-47 1.9110637821823886 1103 48-49 1.8497317875613888 1103 50-51 2.0206019416245127 1103 52-53 1.9403323269409007 1103 54-55 2.1821287972042533 1103 56-57 1.9917312234872533 1103 58-59 2.1648663682240397 1103 60-61 2.1163411244215595 1103 62-63 2.2072399490871533 1103 64-65 2.2537703741584174 1103 66-67 2.507649986744095 1103 68-69 2.569088049908057 1103 70-71 2.368074669439757 1103 72-73 2.4388461827488825 1103 74-75 2.4976162691230037 1103 76-77 2.309654776083878 1103 78-79 2.491096395494548 1103 80-81 2.334094655629933 1103 82-83 2.259029252883913 1103 84-85 2.5603157984748357 1103 86-87 2.389060310420021 1103 88-89 2.3356117245088654 1103 90-91 2.3651702326149007 1103 92-93 2.1658067688478404 1103 94-95 2.019931775574225 1103 96-97 1.9512702730357852 1103 98-99 2.1984064946272817 1103 100-101 2.1495359006029062 1103 102-103 2.017593060158731 1103 104-105 2.042734714028743 1103 106-107 2.0117232085159316 1103 108-109 1.9408506257000333 1103 110-111 1.9896581347028466 1103 112-113 1.8605266483702003 1103 114-115 1.8163009850164187 1103 116-117 1.9340933045791147 1103 118-119 1.9219609552051757 1103 120-121 1.8686341290576962 1103 122-123 1.974763826657231 1103 124-125 1.8395215763907231 1104 1 0.7648743658698329 1104 2 0.6713399356260119 1104 3 0.6694677042791639 1104 4 0.7312714968784633 1104 5 0.9859199591573038 1104 6 1.0471270632159992 1104 7 1.3964370811259137 1104 8 1.1866144638623553 1104 9 1.7439098119248513 1104 10-11 1.2469992998891328 1104 12-13 1.3889475683557357 1104 14-15 1.4598220794670098 1104 16-17 1.2628300702223996 1104 18-19 1.2446224038234561 1104 20-21 1.584364377714671 1104 22-23 1.573947033689521 1104 24-25 1.6985170219342862 1104 26-27 1.621144309511429 1104 28-29 1.57227468381123 1104 30-31 2.0700795314433904 1104 32-33 1.9727761686594683 1104 34-35 1.9720986065682382 1104 36-37 1.8511631539086153 1104 38-39 1.9916557488717679 1104 40-41 2.070312871567687 1104 42-43 2.016581090363374 1104 44-45 1.7381651480021567 1104 46-47 1.8519981428216639 1104 48-49 1.6393342416592915 1104 50-51 2.019665257575703 1104 52-53 1.810663728760371 1104 54-55 2.17976357720125 1104 56-57 2.164372275055687 1104 58-59 2.2095234978045575 1104 60-61 2.2967712044125825 1104 62-63 2.2963853547559125 1104 64-65 2.489417305251301 1104 66-67 2.41265916098855 1104 68-69 2.5385370078057683 1104 70-71 2.3393777244826808 1104 72-73 2.46140136849181 1104 74-75 2.5763291097785093 1104 76-77 2.3729928014817645 1104 78-79 2.4173793619882424 1104 80-81 2.2461473856769842 1104 82-83 2.227197997605394 1104 84-85 2.542015929562705 1104 86-87 2.40928897695364 1104 88-89 2.2558072717884805 1104 90-91 2.366478841505451 1104 92-93 2.1140992171948803 1104 94-95 2.0712436030997026 1104 96-97 2.119814419995837 1104 98-99 2.193348153351014 1104 100-101 2.169879001184295 1104 102-103 2.147070878180287 1104 104-105 1.9734615757661764 1104 106-107 2.1302863812855364 1104 108-109 2.067939617369351 1104 110-111 2.0612954147938147 1104 112-113 2.0566724397303844 1104 114-115 1.9770125906966882 1104 116-117 1.991625189493444 1104 118-119 1.9851688846505162 1104 120-121 2.093557705771886 1104 122-123 2.1881049594678217 1104 124-125 1.9495145435145247 1105 1 0.47751120554672966 1105 2 0.5943827840462852 1105 3 0.48809463836127875 1105 4 0.5450811493998486 1105 5 0.7432494408265207 1105 6 0.8772180241305243 1105 7 1.2569843845773114 1105 8 1.024003870880442 1105 9 1.5878783674633183 1105 10-11 1.258605392714017 1105 12-13 1.3148213530646906 1105 14-15 1.389982525053 1105 16-17 1.3853597842439385 1105 18-19 1.5362927734229714 1105 20-21 1.6639688077884145 1105 22-23 1.6090762088710164 1105 24-25 1.790321629564609 1105 26-27 1.5913013453294127 1105 28-29 1.6104401526462766 1105 30-31 1.9523920678779447 1105 32-33 1.8225474655501195 1105 34-35 1.9852577784988341 1105 36-37 1.674868409049509 1105 38-39 1.8243662450632279 1105 40-41 1.984017191957868 1105 42-43 1.980829546182818 1105 44-45 1.9612046119801825 1105 46-47 1.7818331639249365 1105 48-49 1.8850279302961326 1105 50-51 2.034291424057109 1105 52-53 1.8928308838304488 1105 54-55 2.1518504012682698 1105 56-57 1.9838074576912454 1105 58-59 1.907652983341709 1105 60-61 1.740591247401909 1105 62-63 1.6769042519158361 1105 64-65 2.0606046044295496 1105 66-67 2.2580930533918355 1105 68-69 2.3053061363510317 1105 70-71 2.153973304355059 1105 72-73 2.348565307190583 1105 74-75 2.379457358958188 1105 76-77 2.175878660130884 1105 78-79 2.327138769586522 1105 80-81 2.224532817932424 1105 82-83 2.099733179688389 1105 84-85 2.392936774135304 1105 86-87 2.346276303023245 1105 88-89 2.1735960729223365 1105 90-91 2.226061601939435 1105 92-93 2.0378390995544287 1105 94-95 1.8876589077777872 1105 96-97 1.9886470567940755 1105 98-99 1.965291127110767 1105 100-101 2.0314252561492943 1105 102-103 1.9096927181012333 1105 104-105 1.52172746353531 1105 106-107 1.7433369862948815 1105 108-109 1.7605663566733654 1105 110-111 1.90542891008921 1105 112-113 1.8773099857657094 1105 114-115 1.8032142864173686 1105 116-117 1.7553664739790662 1105 118-119 1.8509262721742203 1105 120-121 1.9194634815182816 1105 122-123 2.0279270206898516 1105 124-125 1.8592033498056288 1106 1 0.40869357577314247 1106 2 0.29018643337673566 1106 3 0.32908759707359536 1106 4 0.17043036867190864 1106 5 0.46162874825328615 1106 6 0.5716249714565542 1106 7 0.8843489353559981 1106 8 0.47848131862521726 1106 9 1.195291299737974 1106 10-11 0.9564520951561519 1106 12-13 0.7791973980962474 1106 14-15 0.9466128618267291 1106 16-17 0.9047685845404061 1106 18-19 0.9632814192371626 1106 20-21 0.8272157956171711 1106 22-23 0.8684243718040179 1106 24-25 0.6118772493175655 1106 26-27 0.7374116539730053 1106 28-29 0.7150066305142246 1106 30-31 1.0795235820903812 1106 32-33 0.9246208665241689 1106 34-35 0.9630594260375887 1106 36-37 0.8524849929890763 1106 38-39 1.084839554993696 1106 40-41 1.2579281268179372 1106 42-43 1.3731312856405822 1106 44-45 1.294287517087664 1106 46-47 1.1638436514458306 1106 48-49 1.0663167223053058 1106 50-51 1.4453835093929612 1106 52-53 1.0639020758302848 1106 54-55 1.4949743051705973 1106 56-57 1.1881987933765359 1106 58-59 1.1315865331953674 1106 60-61 1.4234611630406846 1106 62-63 1.5207597727464304 1106 64-65 1.5206361778786714 1106 66-67 1.6675036920684327 1106 68-69 1.56554588446377 1106 70-71 1.4190535365162091 1106 72-73 1.2672883625199844 1106 74-75 1.5507318232746456 1106 76-77 1.2364157005723264 1106 78-79 1.1190393920095083 1106 80-81 1.2614434055429058 1106 82-83 1.075624429290805 1106 84-85 1.3381946580138795 1106 86-87 1.3880502523014613 1106 88-89 1.1366946480318383 1106 90-91 1.2371140599703807 1106 92-93 1.2110673242336603 1106 94-95 1.0749480557907312 1106 96-97 1.0593761350069357 1106 98-99 1.050168262039115 1106 100-101 0.7809875757764821 1106 102-103 0.6254172879056981 1106 104-105 0.9275063968663062 1106 106-107 0.9728154991328992 1106 108-109 0.8357770554620778 1106 110-111 0.8919858575611457 1106 112-113 0.6230691929861649 1106 114-115 0.6748197758787811 1106 116-117 0.9115861224691137 1106 118-119 0.7200616790836101 1106 120-121 0.7068294329675382 1106 122-123 1.0248709325876675 1106 124-125 0.9352503656477928 1107 1 0.5063458696180234 1107 2 0.5657131325745262 1107 3 0.34953509440290986 1107 4 0.45999744085873573 1107 5 0.5011923930114968 1107 6 0.8320109635204709 1107 7 0.9999741937381792 1107 8 0.9608101091433738 1107 9 1.6145567935409275 1107 10-11 1.1029146237033345 1107 12-13 1.3607315434121467 1107 14-15 1.2065125123703524 1107 16-17 1.1704789161765206 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-4.957785610473227 2216 106-107 -4.9819257312881575 2216 108-109 -5.009779549408446 2216 110-111 -4.7597227391644275 2216 112-113 -4.796302068829263 2216 114-115 -4.6012561264611875 2216 116-117 -4.538557690401159 2216 118-119 -4.753831761563447 2216 120-121 -4.649511123623153 2216 122-123 -4.6843091553846286 2216 124-125 -4.607910965110463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 214424.0 3 71127.0 4 34165.0 5 27629.0 6 27460.0 7 31617.0 8 35551.0 9 37020.0 10 39596.0 11 42259.0 12 45466.0 13 46779.0 14 46805.0 15 38649.0 16 28999.0 17 25444.0 18 22592.0 19 21188.0 20 20951.0 21 22781.0 22 28038.0 23 38617.0 24 53563.0 25 70468.0 26 87046.0 27 99491.0 28 113075.0 29 127985.0 30 142147.0 31 162282.0 32 188867.0 33 224987.0 34 272555.0 35 346410.0 36 488573.0 37 616915.0 38 209154.0 39 795.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.464676167761525 19.777864103140967 13.63528402129584 25.12217570780167 2 15.930508336581184 20.37944704781111 38.57973407922318 25.110310536384535 3 19.073797955904777 24.57462055609218 29.154420000626285 27.197161487376757 4 13.103778939016927 17.069169872729134 36.084755872740715 33.742295315513225 5 13.491919729637935 38.28704049409005 34.14987944029465 14.071160335977376 6 32.516481523278394 36.99008085575532 16.751235165785975 13.742202455180303 7 28.30734041894372 32.010165032375376 20.817303880828344 18.865190667852556 8 25.900753227170135 36.13233384801046 19.25925033783214 18.707662586987258 9 25.599606886235478 15.821070608724138 19.82374917800201 38.75557332703837 10-11 25.154289799614162 26.640207580933943 27.17494961388106 21.030553005570837 12-13 25.73878528502017 23.63342095748394 27.84223461577095 22.78555914172494 14-15 22.59287214709379 25.24293640782797 25.405903701276173 26.758287743802068 16-17 22.68451343054055 28.4779949237631 25.970830607783796 22.86666103791255 18-19 22.608062236294035 27.014065602865923 28.160689732919273 22.217182427920772 20-21 23.145336583529243 26.378208768519173 28.14540945157581 22.331045196375772 22-23 22.82477126341508 26.093374485804876 28.083671232124363 22.998183018655673 24-25 22.745967982825505 26.376999628065807 27.985723412004802 22.891308977103886 26-27 22.503763279879383 26.654126816690386 28.033538417733823 22.808571485696405 28-29 22.449356898950832 26.691454703498067 28.17157053624133 22.687617861309768 30-31 22.509920668376278 26.441212843562006 28.004058356702764 23.04480813135895 32-33 22.41068161655549 26.537642458609227 27.874133228502973 23.17754269633231 34-35 22.87754449202658 26.536294345691037 27.838235031981846 22.747926130300538 36-37 22.951473803827245 26.24008546478402 27.77639203958648 23.032048691802256 38-39 22.64307151736148 26.532703721461903 27.997398015366453 22.826826745810166 40-41 22.750073860474103 26.384093495015005 27.824387795130257 23.04144484938064 42-43 22.764063432095654 26.406373319660943 27.636394819473715 23.193168428769688 44-45 22.85153514210246 26.56753761691622 27.536012647228112 23.0449145937532 46-47 23.033861324034763 26.793271864225183 27.095346169614466 23.07752064212559 48-49 23.027692125365746 26.910462296599107 26.895997537257294 23.165848040777856 50-51 22.95488538991622 26.873036225620524 26.814251010818936 23.35782737364432 52-53 23.22701185557102 26.796148637613797 26.791740556227605 23.185098950587577 54-55 22.933895411004222 26.67251433917964 26.876575838394874 23.51701441142127 56-57 22.86646969974528 27.03918393981296 26.8337380706082 23.260608289833563 58-59 22.933752237310724 26.796240896444235 26.85213678003338 23.41787008621166 60-61 23.172008951046255 26.769264862807635 26.894654182735273 23.16407200341084 62-63 22.949616061118043 26.854572459400856 26.892667656202352 23.303143823278756 64-65 22.87046703682409 26.60647963083721 26.80575870240112 23.717294629937584 66-67 22.627291119655762 27.05290707552456 26.7172115788979 23.60259022592178 68-69 22.79573917540648 26.705573995104785 26.972194701405765 23.526492128082964 70-71 22.657279690279402 26.710583256621423 27.069405901134196 23.56273115196498 72-73 22.91583028781958 26.735069048183497 26.689595750458256 23.659504913538665 74-75 22.876612871366312 26.831959785050053 26.67642989218778 23.614997451395855 76-77 22.865539667098368 26.68748531371235 26.87285622354737 23.574118795641912 78-79 22.87694819538248 26.850818734664834 26.68758488088132 23.584648189071363 80-81 22.64740551195849 26.87951444604669 26.88222433312389 23.59085570887093 82-83 22.943641086614264 26.935220924473875 26.637073090360413 23.484064898551452 84-85 22.694797913702356 26.747507930325387 26.637318019236083 23.920376136736177 86-87 22.76035144740026 26.684247628033557 26.852658239190763 23.702742685375426 88-89 22.725578346800802 26.731975188751523 26.728434234666736 23.814012229780943 90-91 22.651925184383952 26.943090737125104 26.839283871130142 23.56570020736081 92-93 22.747598985395182 26.83580360218434 26.928038213890837 23.488559198529643 94-95 22.63558655510362 26.53396574338493 27.02176298353654 23.808684717974906 96-97 22.804696607477105 26.559617110408723 26.800729733333224 23.83495654878095 98-99 22.328283716370343 26.557580808725582 26.82636511886151 24.28777035604256 100-101 22.379238221551823 26.54439435980534 27.022014410983047 24.054353007659795 102-103 22.613086933397057 26.414833232484014 26.94070300967768 24.03137682444125 104-105 22.532148031312843 26.312047606389992 27.07191290195653 24.083891460340634 106-107 22.19839129175406 26.489175213343234 27.088469318379587 24.223964176523122 108-109 22.40740296899688 26.351452178559903 26.789032172824584 24.452112679618626 110-111 22.213767368431576 26.382287298099715 27.062734412929036 24.341210920539673 112-113 22.48789824505283 26.444743348370597 26.963463973191143 24.103894433385427 114-115 22.2636815021596 26.53836731773835 26.91757098642093 24.28038019368112 116-117 22.07291634047699 26.49542210349587 26.991571660158932 24.440089895868212 118-119 22.046086723494714 26.484491724202304 26.785642236226824 24.683779316076155 120-121 21.94443330349969 26.588183829683498 26.960399782865995 24.50698308395081 122-123 22.092800440139243 26.456735760000782 26.871967824934806 24.578495974925165 124-125 21.905976147444278 26.398020778692054 26.87440942250027 24.821593651363393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 109.0 1 101.0 2 167.5 3 253.0 4 217.5 5 206.5 6 379.5 7 805.5 8 1279.5 9 1515.0 10 1377.0 11 1137.5 12 1159.0 13 1357.5 14 1597.0 15 1911.5 16 2310.0 17 2672.0 18 3132.5 19 3692.5 20 4313.5 21 5147.5 22 6328.5 23 8035.5 24 10252.0 25 13592.0 26 18519.0 27 24519.5 28 30024.0 29 35963.0 30 43526.5 31 51952.5 32 60375.5 33 67845.0 34 75637.0 35 82237.5 36 89993.5 37 100269.0 38 107771.5 39 112611.5 40 116904.0 41 122751.5 42 129083.0 43 135579.0 44 142512.0 45 148363.0 46 151622.5 47 153898.5 48 160591.0 49 164673.0 50 162528.0 51 159236.5 52 155757.0 53 153853.5 54 149446.0 55 138365.0 56 126877.0 57 117428.5 58 108337.0 59 97461.5 60 83171.0 61 67976.5 62 54547.0 63 41878.5 64 30694.5 65 23184.0 66 17904.0 67 14066.5 68 10873.0 69 8345.0 70 6423.0 71 5194.0 72 4315.0 73 3606.5 74 2944.5 75 2214.5 76 1575.5 77 1007.0 78 664.5 79 460.5 80 316.0 81 227.5 82 149.5 83 83.5 84 48.0 85 35.5 86 23.5 87 10.0 88 5.0 89 6.0 90 6.0 91 3.5 92 2.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1052398307105676 2 1.9270282574606105E-4 3 0.0 4 0.17434788159374873 5 0.0 6 7.226355965477289E-5 7 0.037504787460827124 8 0.0 9 0.0 10-11 6.985477433294712E-4 12-13 6.50372036892956E-4 14-15 5.660645506290543E-4 16-17 0.0040106275608398955 18-19 0.0015897983124050035 20-21 0.0031434648449826207 22-23 0.0019029404042423528 24-25 0.004769394937215011 26-27 0.004119022900322055 28-29 0.003360255523946939 30-31 8.671627158572747E-4 32-33 2.408785321825763E-5 34-35 0.0012525683673493967 36-37 0.0012646122939585255 38-39 5.660645506290543E-4 40-41 0.0014814029729228442 42-43 9.273823489029187E-4 44-45 0.0011200851746489798 46-47 1.9270282574606105E-4 48-49 2.408785321825763E-5 50-51 0.038950058653922585 52-53 9.635141287303052E-5 54-55 0.13636133706855644 56-57 2.7701031200996274E-4 58-59 3.6131779827386446E-5 60-61 0.0 62-63 6.744598901112136E-4 64-65 0.06056890691730881 66-67 0.0487779027669717 68-69 0.0016138861656232611 70-71 0.0027821470467087562 72-73 0.06940914904840935 74-75 0.004287637872849858 76-77 0.0015295786793593593 78-79 0.0015416226059684884 80-81 1.3248319270041696E-4 82-83 1.4452711930954578E-4 84-85 2.408785321825763E-5 86-87 2.2883460557344745E-4 88-89 0.0011200851746489798 90-91 2.529224587917051E-4 92-93 0.002408785321825763 94-95 0.003733617248829932 96-97 0.0021799507162523156 98-99 0.0 100-101 0.0012766562205676543 102-103 0.001493446899531973 104-105 2.529224587917051E-4 106-107 6.021963304564407E-5 108-109 1.2043926609128815E-5 110-111 2.0474675235518987E-4 112-113 1.4452711930954578E-4 114-115 1.686149725278034E-4 116-117 0.0 118-119 0.0013850515600498136 120-121 0.002806234899927014 122-123 0.0013609637068315561 124-125 0.008250089727253239 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4151470.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.93093082469563 #Duplication Level Percentage of deduplicated Percentage of total 1 76.14104634560458 39.54075410693535 2 11.584472697169526 12.031849005545716 3 4.65263475239561 7.248469604376938 4 2.313875087643453 4.806467484535947 5 1.4087680054518141 3.65793169195813 6 0.8762143717328851 2.7301576755638757 7 0.5988188915020842 2.1768055701780957 8 0.418932806926545 1.740445649335838 9 0.30622530142713433 1.4312308450665672 >10 1.535751631519076 14.14893173585002 >50 0.10423422410543645 3.733685960982467 >100 0.05548432227205117 5.098119211880371 >500 0.0031637956490072703 1.097148477817037 >1k 2.83324950602436E-4 0.2992445776585099 >5k 9.4441650200812E-5 0.2587584023152173 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5377 0.12952038675457128 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5175 0.12465464040448324 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.408785321825763E-5 0.0 0.0 0.0 0.0 8 2.408785321825763E-5 0.0 0.0 2.408785321825763E-5 0.0 9 2.408785321825763E-5 0.0 0.0 2.408785321825763E-5 0.0 10-11 2.408785321825763E-5 0.0 0.0 4.817570643651526E-5 0.0 12-13 2.408785321825763E-5 0.0 0.0 4.817570643651526E-5 2.408785321825763E-5 14-15 2.408785321825763E-5 0.0 0.0 6.021963304564408E-5 7.226355965477289E-5 16-17 2.408785321825763E-5 0.0 0.0 7.226355965477289E-5 1.2043926609128814E-4 18-19 6.021963304564408E-5 0.0 0.0 8.430748626390171E-5 1.2043926609128814E-4 20-21 9.635141287303052E-5 0.0 0.0 1.5657104591867459E-4 1.3248319270041696E-4 22-23 9.635141287303052E-5 0.0 0.0 2.0474675235518987E-4 1.4452711930954578E-4 24-25 1.2043926609128814E-4 0.0 0.0 2.8905423861909157E-4 1.4452711930954578E-4 26-27 1.2043926609128814E-4 0.0 0.0 3.854056514921221E-4 1.4452711930954578E-4 28-29 1.6861497252780342E-4 0.0 0.0 9.032944956846611E-4 1.4452711930954578E-4 30-31 1.9270282574606105E-4 0.0 0.0 0.0031193769917643626 1.565710459186746E-4 32-33 2.0474675235518987E-4 0.0 0.0 0.0074311027178324785 1.6861497252780342E-4 34-35 2.1679067896431868E-4 0.0 0.0 0.013067660370904764 1.6861497252780342E-4 36-37 2.1679067896431868E-4 0.0 0.0 0.023907194319120695 1.8065889913693222E-4 38-39 2.1679067896431868E-4 0.0 0.0 0.041394975755575734 1.9270282574606105E-4 40-41 2.1679067896431868E-4 0.0 0.0 0.0622791444958051 1.9270282574606105E-4 42-43 2.2883460557344748E-4 1.2043926609128815E-5 0.0 0.08323557679568924 1.9270282574606105E-4 44-45 2.4087853218257628E-4 2.408785321825763E-5 0.0 0.10832307592250456 1.9270282574606105E-4 46-47 2.649663854008339E-4 4.817570643651526E-5 0.0 0.1394325383538843 2.0474675235518987E-4 48-49 2.8905423861909157E-4 4.817570643651526E-5 0.0 0.1751307368233421 2.1679067896431868E-4 50-51 2.8905423861909157E-4 4.817570643651526E-5 0.0 0.2168870303771917 2.1679067896431868E-4 52-53 2.8905423861909157E-4 4.817570643651526E-5 0.0 0.2561381871963425 2.1679067896431868E-4 54-55 2.8905423861909157E-4 4.817570643651526E-5 0.0 0.30123064842092073 2.1679067896431868E-4 56-57 2.8905423861909157E-4 4.817570643651526E-5 0.0 0.35422392550108756 2.1679067896431868E-4 58-59 2.8905423861909157E-4 4.817570643651526E-5 0.0 0.4139979332621939 2.1679067896431868E-4 60-61 2.8905423861909157E-4 4.817570643651526E-5 0.0 0.4828048859801468 2.1679067896431868E-4 62-63 2.8905423861909157E-4 4.817570643651526E-5 0.0 0.5517804536706274 2.1679067896431868E-4 64-65 2.8905423861909157E-4 4.817570643651526E-5 0.0 0.6254651966652776 2.1679067896431868E-4 66-67 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.7088814323601038 2.2883460557344748E-4 68-69 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.8094723073995476 2.529224587917051E-4 70-71 3.6131779827386443E-4 4.817570643651526E-5 0.0 0.9259732094896507 2.649663854008339E-4 72-73 3.6131779827386443E-4 4.817570643651526E-5 0.0 1.0476168682418519 2.8905423861909157E-4 74-75 3.6131779827386443E-4 4.817570643651526E-5 0.0 1.16957366908589 2.8905423861909157E-4 76-77 3.6131779827386443E-4 4.817570643651526E-5 0.0 1.307115310962141 2.8905423861909157E-4 78-79 3.6131779827386443E-4 4.817570643651526E-5 0.0 1.4704309557819277 2.8905423861909157E-4 80-81 3.854056514921221E-4 4.817570643651526E-5 0.0 1.6494037051935821 2.8905423861909157E-4 82-83 3.854056514921221E-4 4.817570643651526E-5 0.0 1.8420463113065972 2.8905423861909157E-4 84-85 4.215374313195085E-4 4.817570643651526E-5 0.0 2.036760472796383 3.010981652282204E-4 86-87 4.5766921114689496E-4 4.817570643651526E-5 0.0 2.2474569248964826 3.1314209183734917E-4 88-89 4.5766921114689496E-4 4.817570643651526E-5 0.0 2.483818984600635 3.1314209183734917E-4 90-91 4.5766921114689496E-4 4.817570643651526E-5 0.0 2.7507726178919754 3.1314209183734917E-4 92-93 4.5766921114689496E-4 4.817570643651526E-5 0.0 3.03923670410722 3.1314209183734917E-4 94-95 4.5766921114689496E-4 4.817570643651526E-5 0.0 3.3358665725634538 3.25186018446478E-4 96-97 4.5766921114689496E-4 4.817570643651526E-5 0.0 3.637386275222993 3.3722994505560683E-4 98-99 4.938009909742813E-4 4.817570643651526E-5 0.0 3.9657157585144542 3.3722994505560683E-4 100-101 5.419766974107967E-4 4.817570643651526E-5 0.0 4.342208904315821 3.3722994505560683E-4 102-103 6.021963304564408E-4 4.817570643651526E-5 0.0 4.7612171110474115 3.3722994505560683E-4 104-105 6.985477433294712E-4 4.817570643651526E-5 0.0 5.174588760125931 3.3722994505560683E-4 106-107 7.346795231568577E-4 4.817570643651526E-5 0.0 5.596583860656587 3.3722994505560683E-4 108-109 7.467234497659865E-4 4.817570643651526E-5 0.0 6.044437271617042 3.3722994505560683E-4 110-111 7.467234497659865E-4 4.817570643651526E-5 0.0 6.539647402004592 3.3722994505560683E-4 112-113 7.467234497659865E-4 4.817570643651526E-5 0.0 7.073843722825891 3.3722994505560683E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5675 0.0 64.98271 1 GTATCAA 9990 0.0 51.787968 1 ATCAACG 12480 0.0 39.017387 3 TATCAAC 13315 0.0 38.226753 2 TCAACGC 12625 0.0 38.1916 4 CAACGCA 12695 0.0 37.630733 5 AACGCAG 12950 0.0 36.3844 6 GTACATG 13770 0.0 34.451527 1 TACATGG 14355 0.0 32.09423 2 ACGCAGA 15665 0.0 29.96446 7 CGCAGAG 16050 0.0 29.382978 8 ACATGGG 14595 0.0 28.993788 3 GCAGAGT 17910 0.0 25.567768 9 CATGGGG 7655 0.0 24.058249 4 GAGTACT 10645 0.0 23.577394 12-13 GTGGTAT 3025 0.0 22.488201 1 AGAGTAC 17420 0.0 22.140676 10-11 GTACTTT 11830 0.0 22.04519 14-15 CAGAGTA 18470 0.0 21.880217 10-11 GAGTACA 10940 0.0 21.763603 1 >>END_MODULE