FastQCFastQC Report
Thu 26 May 2016
SRR1364511_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364511_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658902
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31860.11982389723276751No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77750.053.064041
GGTATCA44950.049.9320221
ATCAACG93850.042.4160733
CAACGCA96650.040.9888155
TCAACGC96250.040.7282074
TATCAAC103300.040.378312
AACGCAG98200.039.3504566
GTACATG135850.035.628571
TACATGG140350.033.1107522
ACGCAGA116750.032.5894367
CGCAGAG113900.032.204958
ACATGGG138900.031.0152243
GCAGAGT121700.028.5261789
CGCATAG3500.027.2218469
CATGGGA86950.026.2421424
GAGTACT51750.024.71406212-13
ACGCATA3800.023.5057058
ATGGGAG37050.022.992885
CAGAGTA125250.022.9514510-11
AGAGTAC120000.022.09567310-11
Produced by FastQC (version 0.11.5)