##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364511_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2658902 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.856410277625876 30.0 26.0 31.0 7.0 34.0 2 26.035552645415287 30.0 26.0 31.0 7.0 34.0 3 26.06191954423292 30.0 26.0 31.0 7.0 34.0 4 28.832908471241137 35.0 27.0 37.0 7.0 37.0 5 29.43447896913839 35.0 28.0 37.0 7.0 37.0 6 29.638578631329775 35.0 29.0 37.0 7.0 37.0 7 29.373699745233182 35.0 29.0 37.0 2.0 37.0 8 29.024731261249944 35.0 28.0 37.0 2.0 37.0 9 29.62203496029564 35.0 27.0 39.0 2.0 39.0 10-11 29.68477420378788 35.0 27.0 39.0 2.0 39.0 12-13 29.53345459892843 35.0 27.0 39.0 2.0 39.0 14-15 30.169690157817023 36.0 27.0 40.0 2.0 41.0 16-17 30.42573325380176 36.0 27.0 40.0 2.0 41.0 18-19 30.382311570716034 36.0 27.0 40.0 2.0 41.0 20-21 30.187080042814664 36.0 27.0 40.0 2.0 41.0 22-23 30.239478175577737 36.0 27.0 40.0 2.0 41.0 24-25 30.273661270704977 36.0 27.0 40.0 2.0 41.0 26-27 30.390905343634326 36.0 27.0 40.0 2.0 41.0 28-29 30.330480213260962 36.0 27.0 40.0 2.0 41.0 30-31 29.97953948660011 36.0 27.0 40.0 2.0 41.0 32-33 29.985842088200318 36.0 27.0 40.0 2.0 41.0 34-35 29.692015162649845 35.5 26.5 40.0 2.0 41.0 36-37 29.44519805543792 35.0 25.0 40.0 2.0 41.0 38-39 29.289468359495764 35.0 25.0 40.0 2.0 41.0 40-41 29.32899802249199 35.0 25.5 40.0 2.0 41.0 42-43 29.16211259384513 35.0 25.0 40.0 2.0 41.0 44-45 28.816356338067365 35.0 24.5 39.5 2.0 41.0 46-47 28.546196700743387 34.5 24.0 39.0 2.0 41.0 48-49 28.280535160754326 34.0 23.5 39.0 2.0 41.0 50-51 28.261037826892455 34.0 23.0 39.0 2.0 41.0 52-53 28.04583282873908 34.0 22.5 39.0 2.0 40.0 54-55 27.9249090414013 34.0 22.5 39.0 2.0 40.0 56-57 27.51044735759347 33.5 21.0 39.0 2.0 40.0 58-59 27.289398217760564 33.0 20.0 38.5 2.0 40.0 60-61 26.876862704981228 33.0 18.0 38.0 2.0 40.0 62-63 26.82992378056807 33.0 18.5 38.0 2.0 40.0 64-65 26.650843092374224 33.0 18.0 38.0 2.0 40.0 66-67 26.42914819726338 33.0 18.0 37.0 2.0 40.0 68-69 26.260698401069313 32.5 17.5 37.0 2.0 40.0 70-71 26.137867999647973 32.0 16.0 36.5 2.0 39.0 72-73 26.106576135562726 33.0 16.5 36.0 2.0 39.0 74-75 25.82432936603154 32.0 14.0 36.0 2.0 39.0 76-77 25.563880127962594 32.0 13.0 35.5 2.0 39.0 78-79 25.248731431244927 32.0 11.0 35.0 2.0 37.5 80-81 25.234162259458977 32.0 12.0 35.0 2.0 37.0 82-83 25.030006559098453 32.0 9.0 35.0 2.0 36.5 84-85 24.843192603563423 32.0 7.0 35.0 2.0 36.0 86-87 24.63057476356782 32.0 7.0 35.0 2.0 36.0 88-89 24.412205677381117 32.0 4.5 35.0 2.0 35.5 90-91 24.244122009761924 32.0 2.0 35.0 2.0 35.0 92-93 24.134756941023024 32.0 2.0 35.0 2.0 35.0 94-95 24.045592692020993 32.0 2.0 35.0 2.0 35.0 96-97 23.91825084188887 32.0 2.0 35.0 2.0 35.0 98-99 23.806347507354538 32.0 2.0 35.0 2.0 35.0 100-101 23.750403926131916 32.0 2.0 35.0 2.0 35.0 102-103 22.62682866837514 29.5 2.0 34.0 2.0 35.0 104-105 23.378031420488607 31.0 2.0 34.5 2.0 35.0 106-107 23.475905843840806 31.5 2.0 35.0 2.0 35.0 108-109 23.404738121224476 32.0 2.0 35.0 2.0 35.0 110-111 23.279801775319285 31.0 2.0 35.0 2.0 35.0 112-113 23.25720485373286 31.5 2.0 35.0 2.0 35.0 114-115 23.199284704739025 32.0 2.0 35.0 2.0 35.0 116-117 23.015852784344816 31.5 2.0 35.0 2.0 35.0 118-119 22.85111561087998 31.0 2.0 35.0 2.0 35.0 120-121 22.696991464897916 31.0 2.0 35.0 2.0 35.0 122-123 22.413027445163454 31.0 2.0 35.0 2.0 35.0 124-125 21.427332786240335 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.9300107476836708 1101 2 1.5578463927650112 1101 3 1.782152677740374 1101 4 2.237364579508636 1101 5 1.5990548496923616 1101 6 1.718622193771612 1101 7 1.506089574334176 1101 8 1.8620270050677625 1101 9 2.3971653162686337 1101 10-11 2.388546505802843 1101 12-13 2.219398494203297 1101 14-15 2.4743135581100653 1101 16-17 2.2213700036623756 1101 18-19 2.1880515957711566 1101 20-21 2.5449752130887475 1101 22-23 2.3700365383914033 1101 24-25 2.3126117388924214 1101 26-27 1.9840316632046004 1101 28-29 1.9053713511395145 1101 30-31 2.0649041453294252 1101 32-33 2.2759852375221854 1101 34-35 2.463994280213395 1101 36-37 2.4351881996094242 1101 38-39 2.4498263410073875 1101 40-41 2.201634162088858 1101 42-43 2.099011318582349 1101 44-45 2.2354999126730597 1101 46-47 2.256020462563125 1101 48-49 2.3172167550772365 1101 50-51 2.258061523852138 1101 52-53 2.135551254926483 1101 54-55 2.154252692176211 1101 56-57 2.3688979158846024 1101 58-59 2.4729478395204936 1101 60-61 2.5433827901241735 1101 62-63 2.2099371239050356 1101 64-65 2.1502736942663176 1101 66-67 2.3040784463613235 1101 68-69 2.1258044241100222 1101 70-71 2.1320124554788364 1101 72-73 2.0801829002746324 1101 74-75 2.024614765083271 1101 76-77 2.2214179418065996 1101 78-79 2.0966214068138136 1101 80-81 1.961746256237081 1101 82-83 2.0232337639239653 1101 84-85 1.791615018972017 1101 86-87 1.7354716964289487 1101 88-89 1.8777648331535879 1101 90-91 1.8750912505779809 1101 92-93 1.8608903015988076 1101 94-95 1.860616108730781 1101 96-97 1.8290131200019601 1101 98-99 1.7571633144787526 1101 100-101 1.7441795342212316 1101 102-103 1.7587484543071916 1101 104-105 1.7896850431118452 1101 106-107 1.9099738000220938 1101 108-109 1.9648047221845886 1101 110-111 2.070166088407909 1101 112-113 2.0170518875161 1101 114-115 1.908286815614204 1101 116-117 2.012999113830233 1101 118-119 2.037891234138165 1101 120-121 2.0414614428148568 1101 122-123 2.0402942507007857 1101 124-125 2.108045977449194 1102 1 0.8522085806745174 1102 2 0.6769011284272572 1102 3 0.7799833334474542 1102 4 1.2390201931832117 1102 5 1.0405199160103145 1102 6 1.0871982259031299 1102 7 1.0515523989185205 1102 8 1.2580759495848568 1102 9 1.5705634107950672 1102 10-11 1.2287132347398853 1102 12-13 1.5302533886550052 1102 14-15 1.7180051796307048 1102 16-17 1.206182258472758 1102 18-19 1.4481428997128774 1102 20-21 1.329434861883815 1102 22-23 1.4106726303024786 1102 24-25 1.1447210701085666 1102 26-27 1.1608250500904624 1102 28-29 0.9619120759667084 1102 30-31 0.8755780664951267 1102 32-33 0.7224183124334473 1102 34-35 1.106686462167442 1102 36-37 1.447978924436626 1102 38-39 1.3655091357019558 1102 40-41 0.9720899812556816 1102 42-43 0.8589648493127804 1102 44-45 1.3676781304948413 1102 46-47 1.2420960276668005 1102 48-49 1.2545976629793465 1102 50-51 1.1885572738988373 1102 52-53 1.2202245689559987 1102 54-55 0.9660089503491953 1102 56-57 1.246757293803526 1102 58-59 1.2123568157954807 1102 60-61 1.587972272657332 1102 62-63 1.4634550110711082 1102 64-65 1.5492474005992136 1102 66-67 1.427399481295005 1102 68-69 1.2125688552701206 1102 70-71 1.4738604846213725 1102 72-73 1.3303989006482553 1102 74-75 1.450463588173129 1102 76-77 1.3254203703513419 1102 78-79 1.1345160453342693 1102 80-81 0.806961556068952 1102 82-83 0.797478346401082 1102 84-85 0.8659063042326167 1102 86-87 0.964406405937634 1102 88-89 0.8129168507138296 1102 90-91 0.7212779683063566 1102 92-93 0.713968015030531 1102 94-95 0.928277933875556 1102 96-97 0.9722563948011356 1102 98-99 1.042983181842846 1102 100-101 0.9293957681087726 1102 102-103 0.6892500422018308 1102 104-105 0.6778844172945426 1102 106-107 0.9777021766333363 1102 108-109 1.1206449052594998 1102 110-111 0.9522228789926288 1102 112-113 0.8099180373386297 1102 114-115 0.7907300717322663 1102 116-117 1.0374877183510662 1102 118-119 1.20017523376454 1102 120-121 1.1018341310270756 1102 122-123 1.2511059450777715 1102 124-125 1.1177111184915987 1103 1 1.3772037193045819 1103 2 1.0662304214220235 1103 3 1.1834868764601616 1103 4 1.9155625206682387 1103 5 1.2120964698546146 1103 6 1.238154874058008 1103 7 1.2083776465590184 1103 8 1.5004261210060186 1103 9 2.0808424881533547 1103 10-11 1.608899998155529 1103 12-13 1.7581282234263007 1103 14-15 2.095595971501563 1103 16-17 1.8583641165346485 1103 18-19 1.5681831738478387 1103 20-21 1.9943006417748776 1103 22-23 1.823134173393889 1103 24-25 1.7440839123432212 1103 26-27 1.550603354259433 1103 28-29 1.5412367853841076 1103 30-31 1.695295146339653 1103 32-33 1.7743815245952028 1103 34-35 1.560087771727904 1103 36-37 2.070052640166857 1103 38-39 1.9654304278077817 1103 40-41 1.9432693785384068 1103 42-43 1.9868236242012607 1103 44-45 1.8598949325467977 1103 46-47 1.9934537388412394 1103 48-49 1.9346276761513437 1103 50-51 1.824060466198432 1103 52-53 1.9938094183808523 1103 54-55 1.8744235765908712 1103 56-57 2.0051401185835473 1103 58-59 2.140170362581536 1103 60-61 2.195992404516204 1103 62-63 2.0451103133210005 1103 64-65 1.9623696848874985 1103 66-67 1.9590211301327898 1103 68-69 1.8313631718656787 1103 70-71 2.0143061854341475 1103 72-73 1.9959710838894935 1103 74-75 2.1144033060284784 1103 76-77 2.1233903722264422 1103 78-79 2.204829858338204 1103 80-81 1.6782645131708342 1103 82-83 1.8340718745287354 1103 84-85 1.8119989781481252 1103 86-87 1.7888523833855068 1103 88-89 1.8678250113334975 1103 90-91 1.8276678339705015 1103 92-93 1.812823249221239 1103 94-95 1.6299689382577363 1103 96-97 1.7583566964375663 1103 98-99 1.7773873572966323 1103 100-101 1.5758552150237684 1103 102-103 1.4937754856860899 1103 104-105 1.743667925444285 1103 106-107 1.6062579504361096 1103 108-109 1.886927716612881 1103 110-111 1.9593689770487757 1103 112-113 1.8304624181737204 1103 114-115 1.7231598365114067 1103 116-117 1.9040511870079584 1103 118-119 2.0524280446780843 1103 120-121 2.025931854979973 1103 122-123 2.021702209462216 1103 124-125 1.9761670374409874 1104 1 1.03184812202365 1104 2 0.7959704633928446 1104 3 0.7985996712492387 1104 4 1.056884845386989 1104 5 0.6245515907681636 1104 6 0.5356822390047675 1104 7 0.6188188565694759 1104 8 0.9889171365822733 1104 9 1.4405809092414685 1104 10-11 1.1423543717417388 1104 12-13 0.6758587437870247 1104 14-15 0.9782741861207818 1104 16-17 0.7576610407304081 1104 18-19 0.09121763840430219 1104 20-21 -4.312393316922737 1104 22-23 -0.9424406446871245 1104 24-25 0.4799470897610263 1104 26-27 0.6039508356515562 1104 28-29 0.0842185067041683 1104 30-31 0.7798808386789027 1104 32-33 0.5764821455510649 1104 34-35 0.8203710532551476 1104 36-37 0.9642742650372256 1104 38-39 0.9421513964358113 1104 40-41 1.0414174124650586 1104 42-43 1.0107567462938896 1104 44-45 1.1948026498132158 1104 46-47 1.1460788516216844 1104 48-49 1.1673064645343487 1104 50-51 1.0375594068870342 1104 52-53 1.0492819774103879 1104 54-55 0.9591559372989558 1104 56-57 1.2244301289256398 1104 58-59 1.2170493314132536 1104 60-61 1.3989340914678117 1104 62-63 0.9572358786576309 1104 64-65 1.0989767470591083 1104 66-67 1.0009690008236447 1104 68-69 1.0010333724391565 1104 70-71 1.160199255945411 1104 72-73 1.0349572576165613 1104 74-75 1.1336695768937552 1104 76-77 1.117582806863986 1104 78-79 1.104656079630292 1104 80-81 0.876758454942415 1104 82-83 0.8398995216238383 1104 84-85 0.8003942468919121 1104 86-87 0.6360519140467282 1104 88-89 0.8627719453549609 1104 90-91 0.8569520206101231 1104 92-93 0.5283913255777222 1104 94-95 0.7148465329251756 1104 96-97 0.8408965622629054 1104 98-99 0.732747817225686 1104 100-101 0.2568949036995356 1104 102-103 0.6665203429695197 1104 104-105 0.8018920404494985 1104 106-107 0.9827961752970111 1104 108-109 1.0183974412492915 1104 110-111 1.0710191073648758 1104 112-113 1.0516110645932173 1104 114-115 1.071370984454969 1104 116-117 1.1386348711739416 1104 118-119 1.1205165936438028 1104 120-121 1.0961745283749735 1104 122-123 0.9630908038987158 1104 124-125 1.1570019641641807 1105 1 1.2382530869299408 1105 2 1.0161608701221567 1105 3 0.9580453868624872 1105 4 1.5066212545242053 1105 5 1.2207124692243134 1105 6 1.082231037187107 1105 7 0.9604536500030214 1105 8 1.2451961980618513 1105 9 1.5785786631633343 1105 10-11 1.3864085741503374 1105 12-13 1.5197876647494404 1105 14-15 1.7353286499853375 1105 16-17 1.4930086925410038 1105 18-19 1.144168073056619 1105 20-21 1.2704196353789783 1105 22-23 1.07455200327896 1105 24-25 1.187564160223797 1105 26-27 1.064512087340848 1105 28-29 0.5858716020546773 1105 30-31 1.05220710971291 1105 32-33 1.0055828379218532 1105 34-35 0.9944405115571833 1105 36-37 1.0956293790451355 1105 38-39 0.8546867022356182 1105 40-41 1.1125865531759942 1105 42-43 1.3695927192714699 1105 44-45 1.5988013850074196 1105 46-47 1.7316148738758095 1105 48-49 1.474332104364592 1105 50-51 1.4765783631723366 1105 52-53 1.5099674571013786 1105 54-55 1.448626787649431 1105 56-57 1.8086533240026057 1105 58-59 1.5554177465026555 1105 60-61 1.8748914623796757 1105 62-63 1.6923061047413874 1105 64-65 1.4900389037580766 1105 66-67 1.7211103775567977 1105 68-69 1.3471162511757022 1105 70-71 1.2897201437884291 1105 72-73 1.29351442082055 1105 74-75 1.7010045158707428 1105 76-77 1.6131246914246269 1105 78-79 1.489993213806283 1105 80-81 1.470690597876004 1105 82-83 1.63459151182591 1105 84-85 1.6079276133452396 1105 86-87 1.585520367250652 1105 88-89 1.6423509295408572 1105 90-91 1.6546747650033602 1105 92-93 1.6319910910854674 1105 94-95 1.5174823631049925 1105 96-97 1.6255577436964153 1105 98-99 1.4560218464906 1105 100-101 1.5703187338914582 1105 102-103 1.643987193368858 1105 104-105 1.5803243671194949 1105 106-107 1.68235750532223 1105 108-109 1.7049083031024885 1105 110-111 1.697669975352703 1105 112-113 1.6245550911651243 1105 114-115 1.58180504392908 1105 116-117 1.744343907116459 1105 118-119 1.7783711598045429 1105 120-121 1.7719111746492722 1105 122-123 1.8030040561071097 1105 124-125 1.4980113870009966 1106 1 -0.6429464888801135 1106 2 -0.47218553619861225 1106 3 -0.37584743136138243 1106 4 -0.7736832538195024 1106 5 -1.2958984703409477 1106 6 -0.7668508514143255 1106 7 -0.7401648398339127 1106 8 -0.33395114786172897 1106 9 -0.4034027921740595 1106 10-11 -2.9925706516733044 1106 12-13 -1.002287178063927 1106 14-15 -0.7660858489552815 1106 16-17 -0.9148225564883212 1106 18-19 -3.613653258366469 1106 20-21 -6.068381347044134 1106 22-23 -6.8110272928170446 1106 24-25 -6.538756959367738 1106 26-27 -5.540023994278219 1106 28-29 -2.3084163835962634 1106 30-31 -4.162826126178096 1106 32-33 -4.734102361170379 1106 34-35 -4.168649773072794 1106 36-37 -6.508368534418302 1106 38-39 -6.899073735141187 1106 40-41 -3.175636991406183 1106 42-43 -1.704690431721577 1106 44-45 -0.9950789166873371 1106 46-47 -0.6695515980351097 1106 48-49 -0.5261228052204103 1106 50-51 -0.724115006133033 1106 52-53 -0.8772912744975905 1106 54-55 -0.6648953065858194 1106 56-57 -0.6064104914078499 1106 58-59 -0.4349461458554167 1106 60-61 -0.1449014622615934 1106 62-63 -0.3728860566561494 1106 64-65 -0.595795386072659 1106 66-67 -0.968842664160988 1106 68-69 -0.7765839581112886 1106 70-71 -0.5451922906703182 1106 72-73 -0.5157574845205914 1106 74-75 -0.6001170171496177 1106 76-77 -0.5562148060881817 1106 78-79 -0.5704009167419244 1106 80-81 -0.8926359606215897 1106 82-83 -0.9939849706292314 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-5.0509105723899665 2216 84-85 -4.807513704361845 2216 86-87 -5.163934906931047 2216 88-89 -5.3447434489636585 2216 90-91 -5.367845375198534 2216 92-93 -5.161460351458025 2216 94-95 -8.205428296238903 2216 96-97 -10.113070737210617 2216 98-99 -7.003767581023883 2216 100-101 -6.10181465071917 2216 102-103 -7.029524129713387 2216 104-105 -9.64884060560231 2216 106-107 -6.438999775784609 2216 108-109 -8.576904371807622 2216 110-111 -6.173593269040417 2216 112-113 -5.655755781248843 2216 114-115 -6.002994182872065 2216 116-117 -5.574799807529978 2216 118-119 -5.892132888433039 2216 120-121 -6.612576942368733 2216 122-123 -9.189473084876958 2216 124-125 -7.817436473974794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 265923.0 3 39716.0 4 23141.0 5 19261.0 6 19550.0 7 21318.0 8 23129.0 9 24966.0 10 26416.0 11 26960.0 12 27501.0 13 28157.0 14 28709.0 15 24784.0 16 20204.0 17 18117.0 18 16668.0 19 15562.0 20 15613.0 21 17136.0 22 21107.0 23 28492.0 24 38539.0 25 48647.0 26 58515.0 27 65971.0 28 74902.0 29 84409.0 30 94431.0 31 105330.0 32 124468.0 33 150279.0 34 183426.0 35 227715.0 36 286491.0 37 284118.0 38 79048.0 39 183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97892394662088 19.051125806870612 13.925593775825037 23.044356470683468 2 15.07274915546419 20.86961887064898 40.35502295173495 23.702609022151876 3 18.787116893828422 26.374757257442756 29.000421801056646 25.837704047672172 4 11.04255255097049 16.733297709808763 38.24743548253569 33.97671425668506 5 13.206944356637734 39.20643557947912 33.417815931047144 14.168804132835996 6 30.77127333479264 37.29899751692306 18.033073591901303 13.896655556382997 7 28.069425869064506 33.61497286782637 20.69266400785908 17.622937255250044 8 26.032211924873693 35.49269245972569 19.926253249080705 18.54884236631991 9 26.52289022763286 16.851098144928127 20.3893164770447 36.23669515039431 10-11 24.371790314571047 27.673554704207927 27.116160151624264 20.838494829596765 12-13 25.23543847118311 25.115474255461155 27.800573925056497 21.84851334829924 14-15 22.371262917717047 26.413886776214774 25.667260221518585 25.547590084549594 16-17 22.207377825895104 29.935613397499246 25.82592670475342 22.03108207185223 18-19 22.062409612856666 28.25740264708665 28.17216201062574 21.508025729430948 20-21 23.114508491505106 27.314426161963222 28.194271355307716 21.37679399122396 22-23 22.63368990243329 27.356746209004353 27.86851298930293 22.141050899259433 24-25 22.193621919292227 27.553658481538324 28.18183698955794 22.07088260961151 26-27 22.18038049848864 27.796215175368832 27.87093224066756 22.15247208547497 28-29 22.064414650672486 27.760832734526286 27.94377526202549 22.230977352775742 30-31 22.19643189592434 27.46471668982844 28.22328200132765 22.115569412919566 32-33 21.985102973481396 27.64536749565627 27.88679959913018 22.48272993173215 34-35 22.386624924413482 27.537755642304372 27.986506807253676 22.08911262602847 36-37 22.549512602968687 27.295136410239433 27.814314481096485 22.34103650569539 38-39 22.235754567965966 27.40474913870778 27.88790779382247 22.47158849950379 40-41 22.486640210445845 27.38790718953068 27.9090179896282 22.21643461039528 42-43 22.24591153958349 27.509109029435336 27.720055964131497 22.52492346684968 44-45 22.270550645317485 27.57144277160829 27.74533849517038 22.412668087903846 46-47 22.37009895691018 27.834534101780008 27.283191730353373 22.51217521095644 48-49 22.43802946826787 27.82356276605648 27.15297113448788 22.585436631187765 50-51 22.477755340815207 27.88344590369333 27.029355558903234 22.609443196588227 52-53 22.775811152850956 27.715287126612402 27.019470527144502 22.48943119339214 54-55 22.496519306166967 27.714748418665735 27.059307863349552 22.729424411817753 56-57 22.354276407963997 28.021130508535137 26.96605314973587 22.658539933764995 58-59 22.501625917482006 27.60621689156174 27.019551258059526 22.87260593289672 60-61 22.469145742103947 27.618917912785097 27.33874701116865 22.57318933394231 62-63 22.41923299199246 27.715448428102423 27.24630611075452 22.619012469150594 64-65 22.209130969861825 27.62623895695922 27.236968008280776 22.927662064898175 66-67 22.1799762961425 27.925679266722558 26.95629229842788 22.93805213870706 68-69 22.294904940019673 27.70958039631567 27.238723516533774 22.75679114713088 70-71 22.056410596840987 27.493221968700983 27.580179357135915 22.87018807732212 72-73 22.363654484799934 27.628240568361743 27.123345631790947 22.884759315047372 74-75 22.23487593738517 27.841942728064932 27.08443734699734 22.838743987552558 76-77 22.278624798536107 27.5613512796836 27.23082825402975 22.929195667750545 78-79 22.363945930105487 27.65123315488347 27.201823145414885 22.78299776959616 80-81 22.250925604143816 27.94713337274955 27.117737900198733 22.684203122907896 82-83 22.35505782115976 27.93775741572617 27.004991239332192 22.702193523781872 84-85 22.333901067562252 27.757145148711952 26.89779599140895 23.011157792316837 86-87 22.218039025713054 27.57666987427154 27.116403335282218 23.088887764733183 88-89 22.284658140778703 27.61181483161484 27.09470626157039 23.008820766036063 90-91 22.312325047179083 27.764844602872962 27.13248036438142 22.79034998556654 92-93 22.2677124722832 27.594152500092616 27.126951271272898 23.011183756351286 94-95 22.193209426022417 27.4595929687525 27.135516796465758 23.211680808759326 96-97 22.239982669146944 27.40847331281814 27.124609134352472 23.226934883682446 98-99 21.936239364812028 27.3163849829444 27.18008307120101 23.56729258104256 100-101 21.917976694526796 27.47866297705653 27.205778508679142 23.397581819737535 102-103 22.1409199618936 27.07117043286417 27.375378497114 23.412531108128224 104-105 21.901205124246292 27.267311237539595 27.393760614629727 23.43772302358439 106-107 21.802667404823055 27.338859100826983 27.47577783323144 23.38269566111852 108-109 21.91860472769485 27.251219458532017 27.1328794679082 23.697296345864935 110-111 21.701959772516396 27.525576108881015 27.366615152760858 23.40584896584173 112-113 21.74645400740792 27.58933057918381 27.416450276367772 23.247765137040503 114-115 21.501517212120845 27.657189795187648 27.269819288527557 23.571473704163946 116-117 21.441260024003824 27.57000206488555 27.486315901913045 23.50242200919758 118-119 21.53346448289668 27.579771524447246 27.2183486024598 23.66841539019628 120-121 21.56287165692131 27.678337023103754 27.250176966053356 23.508614353921576 122-123 21.54604025936903 27.5784952383818 27.358355022642133 23.517109479607036 124-125 21.52277733018088 27.61835718433161 27.07942835876106 23.779437126726453 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1425.0 1 1445.5 2 1652.0 3 1754.0 4 1429.0 5 1059.5 6 951.5 7 1054.5 8 1284.5 9 1415.0 10 1345.0 11 1293.0 12 1353.5 13 1476.0 14 1618.5 15 1789.0 16 1984.5 17 2222.0 18 2522.5 19 2902.0 20 3401.5 21 4101.5 22 5146.0 23 6551.5 24 8380.5 25 10985.0 26 14720.0 27 19238.0 28 23829.5 29 28846.0 30 34669.5 31 40794.5 32 47256.5 33 52928.0 34 57988.0 35 63446.0 36 69634.5 37 75274.5 38 77898.5 39 79494.0 40 81225.0 41 83614.5 42 85849.0 43 88146.5 44 90917.0 45 92602.5 46 93182.5 47 93468.5 48 94076.0 49 93044.5 50 90665.5 51 88278.5 52 86021.5 53 84718.0 54 82739.5 55 78002.0 56 72132.5 57 66180.0 58 61344.0 59 55931.0 60 48193.5 61 40617.0 62 33753.0 63 26487.0 64 20660.0 65 16726.5 66 13057.0 67 10331.0 68 7723.5 69 5299.5 70 3988.5 71 3134.0 72 2477.5 73 2114.5 74 1719.5 75 1370.0 76 1040.5 77 680.0 78 460.5 79 306.5 80 199.0 81 138.0 82 103.0 83 72.0 84 54.5 85 46.0 86 31.5 87 24.5 88 23.5 89 21.0 90 13.0 91 9.0 92 7.0 93 7.5 94 6.5 95 3.0 96 2.5 97 5.0 98 4.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10556989313633973 2 0.03508967235347523 3 0.04704949637105843 4 0.05573729306307642 5 0.05829473970834578 6 0.003911388986882556 7 0.002482227626290852 8 0.0027454941927156397 9 0.0071834163124477705 10-11 0.06797918840182902 12-13 0.007239830576681652 14-15 0.012937671264303835 16-17 0.022001563051214375 18-19 0.02051598742638879 20-21 0.1398697657905406 22-23 0.017469617157759104 24-25 0.015871213004465753 26-27 0.007728754200041972 28-29 0.005396964611708141 30-31 0.0025198371357801077 32-33 0.006901344991278356 34-35 0.019726187727114423 36-37 0.003159198797097448 38-39 0.009458791636547717 40-41 0.03482640578705044 42-43 0.015551532173807081 44-45 0.11282852846776603 46-47 0.004456726874476758 48-49 0.010511857902246867 50-51 0.012881257000069955 52-53 0.01395312802051373 54-55 0.011602533677435273 56-57 0.007032978274490749 58-59 0.0151566323241699 60-61 0.03315278261477858 62-63 0.008292896842380803 64-65 0.008048435030700643 66-67 0.009891300995674155 68-69 0.008969868013187398 70-71 0.004832821969369311 72-73 0.0012035043036561707 74-75 0.0016172089080379794 76-77 0.008913453748953516 78-79 0.02403247656363416 80-81 0.0036857319299470237 82-83 0.005396964611708141 84-85 0.0032908320803098426 86-87 0.0043815078554982475 88-89 0.006619273670108939 90-91 0.005622621668643673 92-93 0.003403660608777608 94-95 0.004268679327030481 96-97 0.00225657056935532 98-99 0.0037045366846916506 100-101 0.0031780035518420764 102-103 0.0011658947941669154 104-105 0.004908040988347822 106-107 9.590424919760112E-4 108-109 0.0014103566058470752 110-111 0.0027454941927156397 112-113 0.004738798195646173 114-115 0.0029335417401619166 116-117 0.005979912008791599 118-119 0.003967803251116438 120-121 0.007521901897851069 122-123 0.005340550347474258 124-125 0.056451873743372266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2658902.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.01465672610049 #Duplication Level Percentage of deduplicated Percentage of total 1 81.24215742793314 50.382045045810884 2 10.040710818141438 12.453424693461699 3 3.6384896299572818 6.769190562098316 4 1.704714254899515 4.228690773347344 5 0.9363844931456311 2.9034781453034957 6 0.5799975555521636 2.15810095857269 7 0.36820398602938703 1.5983830659156035 8 0.25891535594403964 1.2845237535988592 9 0.1831299355851627 1.0221066082428083 >10 0.9527354309129799 10.595954866182069 >50 0.06408722762301046 2.7080454382505623 >100 0.02927106520138857 3.156923804285569 >500 8.229814723252316E-4 0.3158007170592124 >1k 3.7983760259875307E-4 0.4233315678710029 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3186 0.11982389723276751 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.760950948925534E-5 2 0.0 0.0 0.0 0.0 3.760950948925534E-5 3 0.0 0.0 0.0 0.0 3.760950948925534E-5 4 0.0 0.0 0.0 0.0 3.760950948925534E-5 5 0.0 0.0 0.0 0.0 3.760950948925534E-5 6 0.0 0.0 3.760950948925534E-5 0.0 7.521901897851068E-5 7 0.0 0.0 3.760950948925534E-5 0.0 7.521901897851068E-5 8 0.0 0.0 3.760950948925534E-5 0.0 7.521901897851068E-5 9 0.0 0.0 3.760950948925534E-5 3.760950948925534E-5 1.880475474462767E-4 10-11 0.0 0.0 3.760950948925534E-5 3.760950948925534E-5 1.880475474462767E-4 12-13 1.880475474462767E-5 0.0 3.760950948925534E-5 5.6414264233883016E-5 2.2565705693553204E-4 14-15 3.760950948925534E-5 0.0 3.760950948925534E-5 7.521901897851068E-5 3.0087607591404274E-4 16-17 3.760950948925534E-5 0.0 3.760950948925534E-5 1.1282852846776603E-4 3.5729034014792574E-4 18-19 9.402377372313835E-5 0.0 3.760950948925534E-5 1.880475474462767E-4 4.1370460438180874E-4 20-21 1.5043803795702137E-4 0.0 3.760950948925534E-5 1.880475474462767E-4 4.1370460438180874E-4 22-23 1.5043803795702137E-4 0.0 3.760950948925534E-5 3.3848558540329807E-4 4.325093591264364E-4 24-25 1.5043803795702137E-4 0.0 3.760950948925534E-5 7.333854350404791E-4 5.077283781049471E-4 26-27 1.5043803795702137E-4 0.0 3.760950948925534E-5 0.0012975280773793092 5.265331328495748E-4 28-29 1.5043803795702137E-4 0.0 3.760950948925534E-5 0.002331789588333831 5.265331328495748E-4 30-31 1.5043803795702137E-4 3.760950948925534E-5 3.760950948925534E-5 0.00573545019711144 5.265331328495748E-4 32-33 1.5043803795702137E-4 3.760950948925534E-5 3.760950948925534E-5 0.011583728922690644 5.641426423388301E-4 34-35 1.5043803795702137E-4 3.760950948925534E-5 3.760950948925534E-5 0.020045868557773096 5.641426423388301E-4 36-37 1.5043803795702137E-4 3.760950948925534E-5 3.760950948925534E-5 0.034337482163690126 5.641426423388301E-4 38-39 1.880475474462767E-4 3.760950948925534E-5 3.760950948925534E-5 0.05795625412294248 6.017521518280855E-4 40-41 1.880475474462767E-4 3.760950948925534E-5 3.760950948925534E-5 0.08845756631872856 6.017521518280855E-4 42-43 2.0685230219090437E-4 3.760950948925534E-5 3.760950948925534E-5 0.11711601254954113 6.393616613173408E-4 44-45 2.2565705693553204E-4 3.760950948925534E-5 3.760950948925534E-5 0.15072010927819077 7.333854350404791E-4 46-47 2.2565705693553204E-4 3.760950948925534E-5 3.760950948925534E-5 0.19248546956600882 8.274092087636175E-4 48-49 2.632665664247874E-4 3.760950948925534E-5 3.760950948925534E-5 0.23808699982173093 9.778472467206387E-4 50-51 2.632665664247874E-4 3.760950948925534E-5 3.760950948925534E-5 0.2921882792220247 9.778472467206387E-4 52-53 2.632665664247874E-4 3.760950948925534E-5 3.760950948925534E-5 0.34435266888362187 0.0010342615109545218 54-55 2.632665664247874E-4 3.760950948925534E-5 3.760950948925534E-5 0.4024969705540107 0.001090675775188405 56-57 2.632665664247874E-4 3.760950948925534E-5 3.760950948925534E-5 0.4685956834813769 0.001090675775188405 58-59 2.632665664247874E-4 3.760950948925534E-5 3.760950948925534E-5 0.5491364480526172 0.001090675775188405 60-61 2.632665664247874E-4 3.760950948925534E-5 3.760950948925534E-5 0.6421823745290349 0.0011094805299330327 62-63 2.632665664247874E-4 3.760950948925534E-5 3.760950948925534E-5 0.7355103723266221 0.0011658947941669156 64-65 3.0087607591404274E-4 3.760950948925534E-5 3.760950948925534E-5 0.8303991647680133 0.001203504303656171 66-67 3.0087607591404274E-4 3.760950948925534E-5 3.760950948925534E-5 0.93611949594231 0.001203504303656171 68-69 3.0087607591404274E-4 3.760950948925534E-5 3.760950948925534E-5 1.06314561424227 0.0012411138131454263 70-71 3.5729034014792574E-4 3.760950948925534E-5 3.760950948925534E-5 1.20935258238175 0.0012411138131454263 72-73 3.760950948925534E-4 3.760950948925534E-5 3.760950948925534E-5 1.3660337989139877 0.0013915518511024476 74-75 3.760950948925534E-4 3.760950948925534E-5 3.760950948925534E-5 1.5216243396710372 0.001429161360591703 76-77 3.760950948925534E-4 3.760950948925534E-5 3.760950948925534E-5 1.6943836215099315 0.001429161360591703 78-79 4.1370460438180874E-4 3.760950948925534E-5 3.760950948925534E-5 1.8999007861139674 0.001429161360591703 80-81 4.701188686156917E-4 3.760950948925534E-5 3.760950948925534E-5 2.1337755208729017 0.0014667708700809583 82-83 5.265331328495748E-4 3.760950948925534E-5 3.760950948925534E-5 2.3751345480201977 0.0015795993985487243 84-85 5.265331328495748E-4 3.760950948925534E-5 3.760950948925534E-5 2.6158730182609213 0.0015795993985487243 86-87 5.265331328495748E-4 3.760950948925534E-5 3.760950948925534E-5 2.875322219472549 0.0016360136627826074 88-89 5.265331328495748E-4 3.760950948925534E-5 3.760950948925534E-5 3.163016162310608 0.001767646945995001 90-91 6.393616613173408E-4 3.760950948925534E-5 3.760950948925534E-5 3.4918549085299118 0.001767646945995001 92-93 6.393616613173408E-4 3.760950948925534E-5 3.760950948925534E-5 3.846343340220888 0.0018052564554842563 94-95 6.393616613173408E-4 3.760950948925534E-5 3.760950948925534E-5 4.199327391532294 0.0018428659649735116 96-97 6.393616613173408E-4 3.760950948925534E-5 3.760950948925534E-5 4.5548688894889695 0.0018992802292073945 98-99 6.769711708065961E-4 3.760950948925534E-5 3.760950948925534E-5 4.946195835724671 0.0019556944934412774 100-101 6.957759255512238E-4 3.760950948925534E-5 3.760950948925534E-5 5.39829974929501 0.0019556944934412774 102-103 7.145806802958515E-4 3.760950948925534E-5 3.760950948925534E-5 5.87657988146987 0.0020873277766536714 104-105 9.214329824867558E-4 3.760950948925534E-5 3.760950948925534E-5 6.347883449634473 0.002162546795632182 106-107 9.966520014652665E-4 3.760950948925534E-5 3.760950948925534E-5 6.822647092672089 0.0022189610598660654 108-109 0.0010154567562098943 3.760950948925534E-5 3.760950948925534E-5 7.32381261137116 0.0022941800788445757 110-111 0.0010530662656991496 3.760950948925534E-5 3.760950948925534E-5 7.888519396352329 0.002350594343078459 112-113 0.0010530662656991496 3.760950948925534E-5 3.760950948925534E-5 8.489425334216905 0.0023693990978230863 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7775 0.0 53.06404 1 GGTATCA 4495 0.0 49.932022 1 ATCAACG 9385 0.0 42.416073 3 CAACGCA 9665 0.0 40.988815 5 TCAACGC 9625 0.0 40.728207 4 TATCAAC 10330 0.0 40.37831 2 AACGCAG 9820 0.0 39.350456 6 GTACATG 13585 0.0 35.62857 1 TACATGG 14035 0.0 33.110752 2 ACGCAGA 11675 0.0 32.589436 7 CGCAGAG 11390 0.0 32.20495 8 ACATGGG 13890 0.0 31.015224 3 GCAGAGT 12170 0.0 28.526178 9 CGCATAG 350 0.0 27.221846 9 CATGGGA 8695 0.0 26.242142 4 GAGTACT 5175 0.0 24.714062 12-13 ACGCATA 380 0.0 23.505705 8 ATGGGAG 3705 0.0 22.99288 5 CAGAGTA 12525 0.0 22.95145 10-11 AGAGTAC 12000 0.0 22.095673 10-11 >>END_MODULE