FastQCFastQC Report
Thu 26 May 2016
SRR1364511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658902
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92380.34743664866174084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85390.3211476015287513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50730.19079304163899233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66750.066.050851
GTATCAA116850.054.1205751
ATCAACG153900.039.111953
TATCAAC162050.038.5791972
TCAACGC158550.037.739334
CAACGCA158250.037.631945
AACGCAG162000.036.4303136
GTACATG136450.032.180261
GTGGTAT29100.031.572791
CGCAGAG193200.031.0595448
ACGCAGA192450.030.5116357
TACATGG140200.030.4787982
TCACCGC6200.028.8374124
GCAGAGT204100.028.2061219
TGGTATC31950.027.7934272
ACATGGG142850.027.4518953
GAGTACT126950.026.14238412-13
AGTACTT133600.025.53116612-13
ACCGCAG7250.025.4384086
CACCGCA7050.025.3161935
Produced by FastQC (version 0.11.5)