##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364511_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2658902 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.03093344545982 31.0 28.0 31.0 25.0 34.0 2 29.190491037277795 31.0 28.0 33.0 25.0 34.0 3 29.261563607835114 31.0 28.0 34.0 25.0 34.0 4 32.702385044653774 35.0 32.0 37.0 26.0 37.0 5 31.879759013306998 35.0 32.0 37.0 22.0 37.0 6 31.94651250779457 35.0 32.0 37.0 22.0 37.0 7 31.381555995670393 35.0 32.0 37.0 17.0 37.0 8 31.510033841036638 35.0 32.0 37.0 17.0 37.0 9 32.07228660552364 37.0 31.0 39.0 12.0 39.0 10-11 32.273399320471384 37.0 31.5 39.0 17.0 39.0 12-13 32.021303530555095 37.0 30.5 39.0 13.5 39.0 14-15 32.96269475144251 38.0 31.0 40.0 15.0 41.0 16-17 33.05059268826004 38.0 32.0 40.0 16.0 41.0 18-19 32.95260393199899 38.0 31.0 40.0 13.0 41.0 20-21 32.855442584946715 38.0 31.0 40.0 12.5 41.0 22-23 32.81207129860371 38.0 31.0 40.0 12.0 41.0 24-25 32.658618106270936 38.0 31.0 40.0 10.0 41.0 26-27 32.688859536756155 37.5 31.0 40.0 10.0 41.0 28-29 32.54998604687198 37.0 31.0 40.0 10.0 41.0 30-31 32.00206664254644 37.0 30.0 40.0 8.5 41.0 32-33 31.898428185769916 37.0 30.0 40.0 8.0 41.0 34-35 31.72450921470592 37.0 30.0 40.0 7.5 41.0 36-37 31.645042201630595 37.0 30.0 40.0 2.0 41.0 38-39 31.413622991746216 36.0 30.0 40.0 2.0 41.0 40-41 31.070188182941678 36.0 29.5 40.0 2.0 41.0 42-43 30.93598729851646 36.0 29.0 40.0 2.0 41.0 44-45 30.817049857422347 36.0 29.0 40.0 2.0 41.0 46-47 30.581312887801055 36.0 28.5 40.0 2.0 41.0 48-49 30.24220016382702 36.0 28.0 40.0 2.0 41.0 50-51 29.904343221374837 35.0 27.0 40.0 2.0 41.0 52-53 29.77724320038873 35.0 27.0 40.0 2.0 41.0 54-55 29.301709126549227 35.0 26.0 39.5 2.0 41.0 56-57 29.31587738096402 35.0 26.0 39.0 2.0 41.0 58-59 29.097336983461595 35.0 26.0 39.0 2.0 41.0 60-61 28.835218071218872 34.5 26.0 39.0 2.0 41.0 62-63 28.5745864270289 34.0 25.5 39.0 2.0 40.5 64-65 28.085828473557882 34.0 23.0 38.0 2.0 40.0 66-67 27.74021099687014 34.0 22.5 38.0 2.0 40.0 68-69 27.55250870472097 34.0 22.5 37.0 2.0 40.0 70-71 27.62354084505559 34.0 24.0 37.0 2.0 40.0 72-73 27.432115023419442 34.0 23.5 36.5 2.0 39.0 74-75 27.064916457996574 34.0 22.5 36.0 2.0 39.0 76-77 26.965777790982894 33.0 23.5 36.0 2.0 39.0 78-79 26.571342794883 33.0 22.0 35.0 2.0 37.5 80-81 26.35345943551135 33.0 21.5 35.0 2.0 37.0 82-83 26.195238297613074 33.0 21.5 35.0 2.0 37.0 84-85 25.79645432588339 33.0 20.0 35.0 2.0 36.0 86-87 25.66790088540307 33.0 20.0 35.0 2.0 36.0 88-89 25.597250293542224 33.0 20.0 35.0 2.0 36.0 90-91 25.430341546999475 33.0 19.5 35.0 2.0 35.0 92-93 25.45744295953743 33.0 20.0 35.0 2.0 35.0 94-95 25.43766975992346 33.0 20.0 35.0 2.0 35.0 96-97 25.315623704822514 33.0 19.0 35.0 2.0 35.0 98-99 25.19945375948418 33.0 18.5 35.0 2.0 35.0 100-101 25.11362566202139 33.0 18.0 35.0 2.0 35.0 102-103 23.957506519608472 30.5 16.0 34.0 2.0 35.0 104-105 24.890246989170716 32.0 18.0 35.0 2.0 35.0 106-107 25.02241808836881 33.0 18.0 35.0 2.0 35.0 108-109 25.076935893086695 33.0 18.0 35.0 2.0 35.0 110-111 25.01133475396987 33.0 17.0 35.0 2.0 35.0 112-113 24.939290917829993 33.0 16.0 35.0 2.0 35.0 114-115 24.882878157976485 33.0 11.5 35.0 2.0 35.0 116-117 24.797937644937647 33.0 7.0 35.0 2.0 35.0 118-119 24.66694748433752 33.0 2.0 35.0 2.0 35.0 120-121 24.502800968219212 33.0 2.0 35.0 2.0 35.0 122-123 24.21004384516616 33.0 2.0 35.0 2.0 35.0 124-125 23.432715835333532 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.970792583074644 1101 2 0.9496040234024541 1101 3 0.8750706215721173 1101 4 0.9713075230218848 1101 5 1.1179597348754093 1101 6 1.1875636968712797 1101 7 1.4838842005881574 1101 8 1.2190207819126222 1101 9 2.0115233810025686 1101 10-11 1.7975466988644726 1101 12-13 1.8926724559800903 1101 14-15 1.787069378966244 1101 16-17 1.752924682803794 1101 18-19 1.7816570492391506 1101 20-21 2.04263287249249 1101 22-23 2.036663869059133 1101 24-25 2.2489249963325335 1101 26-27 1.8955332719722122 1101 28-29 1.9691013003618068 1101 30-31 2.516702595433319 1101 32-33 2.4251359207473016 1101 34-35 2.364197690689597 1101 36-37 2.127213900578191 1101 38-39 2.2767559122037575 1101 40-41 2.4209389190223654 1101 42-43 2.3615038900640783 1101 44-45 2.193499424792936 1101 46-47 1.9820009325423449 1101 48-49 2.027688080435624 1101 50-51 2.3669305724178926 1101 52-53 2.2656037762569667 1101 54-55 2.551925730722566 1101 56-57 2.3626008259399 1101 58-59 2.3934168215925986 1101 60-61 2.539763633977522 1101 62-63 2.4873143438833374 1101 64-65 2.5822801065915186 1101 66-67 2.7355173022103045 1101 68-69 2.6872144720707816 1101 70-71 2.3529343776157177 1101 72-73 2.4528392065544864 1101 74-75 2.531642622426645 1101 76-77 2.320391459209425 1101 78-79 2.380562787176679 1101 80-81 2.439689373053099 1101 82-83 2.3339277451617413 1101 84-85 2.5091861498884462 1101 86-87 2.372737830764546 1101 88-89 2.247736244314172 1101 90-91 2.201404963896902 1101 92-93 2.1349956306954034 1101 94-95 2.1373007741991508 1101 96-97 2.018717751606644 1101 98-99 2.062975299800062 1101 100-101 2.1970989603424584 1101 102-103 2.04942375260568 1101 104-105 2.133845667306396 1101 106-107 2.29254610958165 1101 108-109 2.2317364059686717 1101 110-111 2.2244052608447333 1101 112-113 2.1550433906964237 1101 114-115 2.135858880072302 1101 116-117 2.138216052299363 1101 118-119 2.0854133909065418 1101 120-121 2.120891953137523 1101 122-123 2.158342720139249 1101 124-125 2.040259835327909 1102 1 0.3762988412476318 1102 2 -1.6198033182751104 1102 3 -0.09439042830645761 1102 4 0.40408587161145704 1102 5 0.7628107529960921 1102 6 0.3904172369422696 1102 7 1.0094493042683368 1102 8 0.7544168215165818 1102 9 1.5580323479369937 1102 10-11 1.300382725391664 1102 12-13 1.48834894579889 1102 14-15 1.4613279722784043 1102 16-17 1.4604870310197455 1102 18-19 1.4919888725158117 1102 20-21 1.6339625007383276 1102 22-23 1.5672261705723045 1102 24-25 1.76897333371344 1102 26-27 1.3764855417274902 1102 28-29 1.3711060173429388 1102 30-31 1.832709974473115 1102 32-33 1.393052276062079 1102 34-35 1.6579220041586815 1102 36-37 1.1140086885475569 1102 38-39 1.0495608813799144 1102 40-41 1.359613494027034 1102 42-43 1.1329871704675938 1102 44-45 1.1409541230929534 1102 46-47 0.7460771514849931 1102 48-49 0.9653025022551631 1102 50-51 1.0961583418929521 1102 52-53 0.9302217474880514 1102 54-55 1.1435227324038664 1102 56-57 0.8132593351870483 1102 58-59 0.8309576865281478 1102 60-61 0.9001725168469434 1102 62-63 1.1598846315724813 1102 64-65 1.3007533925993648 1102 66-67 1.4321395233946852 1102 68-69 1.3789364036979066 1102 70-71 0.7560272694520727 1102 72-73 1.1513295390783043 1102 74-75 1.3257475635811389 1102 76-77 1.2501341268724175 1102 78-79 1.2122541284806232 1102 80-81 1.1389491339348474 1102 82-83 0.6827193106390403 1102 84-85 0.7962016085097794 1102 86-87 0.8428083519676086 1102 88-89 0.9190175351791083 1102 90-91 0.6640847696133214 1102 92-93 0.7748751375140088 1102 94-95 0.794257153799375 1102 96-97 0.9027944375771249 1102 98-99 0.7077796151372553 1102 100-101 0.5014061245494439 1102 102-103 0.30892893661464527 1102 104-105 0.6587580995873559 1102 106-107 0.8944305668953092 1102 108-109 0.7510736934710067 1102 110-111 0.7168756045772184 1102 112-113 0.7400833215763036 1102 114-115 0.9622938230949067 1102 116-117 0.49931613636437433 1102 118-119 0.09396934084115927 1102 120-121 0.39631811603683076 1102 122-123 0.7551716011405567 1102 124-125 0.7921664764637235 1103 1 0.7730054946111196 1103 2 0.637427275020368 1103 3 0.5180984889275635 1103 4 0.6558694735161907 1103 5 0.887207653757013 1103 6 0.9606259517581037 1103 7 1.269814207955335 1103 8 1.1098554791302924 1103 9 1.907924876937379 1103 10-11 1.4814870434456324 1103 12-13 1.4922028257508302 1103 14-15 1.3263852843575208 1103 16-17 1.3720111688789345 1103 18-19 1.4678852454708604 1103 20-21 1.643585698269682 1103 22-23 1.5410361477238652 1103 24-25 1.8827006270647004 1103 26-27 1.3352173434206307 1103 28-29 1.5944138265550123 1103 30-31 2.159287604763616 1103 32-33 1.8337714868070947 1103 34-35 1.9836454223036277 1103 36-37 1.9028088445743805 1103 38-39 1.9265052846699042 1103 40-41 2.202757862824452 1103 42-43 2.1110318059458777 1103 44-45 1.9214313533621556 1103 46-47 1.8713502273663458 1103 48-49 1.7860156420751103 1103 50-51 1.8879108541794878 1103 52-53 1.8666411922733097 1103 54-55 1.9951149757408935 1103 56-57 1.9170117051048905 1103 58-59 2.017258172433646 1103 60-61 1.9378014878338234 1103 62-63 2.032024062607004 1103 64-65 2.1644675197403913 1103 66-67 2.427461901669453 1103 68-69 2.5561587153024625 1103 70-71 2.2484121360074667 1103 72-73 2.3459040476748285 1103 74-75 2.3238301293195924 1103 76-77 2.162889782844843 1103 78-79 2.338513194356814 1103 80-81 2.2970971950584733 1103 82-83 2.133712216511345 1103 84-85 2.3234893171146354 1103 86-87 2.165790623014445 1103 88-89 2.230350661711306 1103 90-91 2.248338705586768 1103 92-93 2.050879791696989 1103 94-95 1.9689884763003427 1103 96-97 1.9738904392161025 1103 98-99 2.158751136769194 1103 100-101 2.02370951107234 1103 102-103 1.8525862088768257 1103 104-105 1.9562393891004533 1103 106-107 1.974662442666471 1103 108-109 1.9030410146478296 1103 110-111 1.9846449534082922 1103 112-113 1.9415300999927503 1103 114-115 1.7505590258719046 1103 116-117 1.803598048364318 1103 118-119 1.8713428468380044 1103 120-121 1.8268292890853992 1103 122-123 1.8801869086685095 1103 124-125 1.7919496825140442 1104 1 0.4920154365031948 1104 2 0.4458819713487081 1104 3 0.47870530151192625 1104 4 0.49426746763798235 1104 5 0.8067143316503227 1104 6 0.8530179873429304 1104 7 1.2122119094575936 1104 8 0.9357267753057279 1104 9 1.3850625415179394 1104 10-11 0.8786154991173518 1104 12-13 0.9327067481435449 1104 14-15 0.9538626888222623 1104 16-17 0.8031831818287856 1104 18-19 0.7876262341519364 1104 20-21 1.2092396957537304 1104 22-23 1.1091107111971965 1104 24-25 1.3585297516015729 1104 26-27 1.2201027556197133 1104 28-29 1.0723153141810684 1104 30-31 1.6853780787068509 1104 32-33 1.4322085542429903 1104 34-35 1.4417983188784014 1104 36-37 1.3146529070515172 1104 38-39 1.356188739186937 1104 40-41 1.5196393562168389 1104 42-43 1.434626335681898 1104 44-45 1.2338184832665817 1104 46-47 1.203216996545482 1104 48-49 1.0254322103651958 1104 50-51 1.3762193411500263 1104 52-53 1.0691586526226438 1104 54-55 1.4857672877938057 1104 56-57 1.4774501897373469 1104 58-59 1.6240212696679635 1104 60-61 1.6063151957679693 1104 62-63 1.648335306512557 1104 64-65 1.8372733394265168 1104 66-67 1.6926146447624149 1104 68-69 1.9160230058220158 1104 70-71 1.638140831799337 1104 72-73 1.7184076372845425 1104 74-75 1.8125255482460503 1104 76-77 1.6410218517940365 1104 78-79 1.6967827801423923 1104 80-81 1.6577851952191267 1104 82-83 1.6398034363475062 1104 84-85 1.9488405681348588 1104 86-87 1.9073616743361832 1104 88-89 1.7373362358552171 1104 90-91 1.7364420720023617 1104 92-93 1.4445534721478523 1104 94-95 1.3880006415270998 1104 96-97 1.4062759625819155 1104 98-99 1.453912084684351 1104 100-101 1.466289382577937 1104 102-103 1.448032532557061 1104 104-105 1.378860169964284 1104 106-107 1.4104923417844546 1104 108-109 1.3174835098891755 1104 110-111 1.4347027601101416 1104 112-113 1.3912422140110898 1104 114-115 1.2718635926013526 1104 116-117 1.2156673131290532 1104 118-119 1.2404052881168681 1104 120-121 1.2695687386451056 1104 122-123 1.4320748784196802 1104 124-125 1.1312961799012662 1105 1 0.42844291934577683 1105 2 0.5305292618838813 1105 3 0.40984970420833733 1105 4 0.637399082414845 1105 5 0.795271234855246 1105 6 0.8785709644499065 1105 7 1.2183810036600775 1105 8 0.9980043962560039 1105 9 1.6823509641966226 1105 10-11 1.3393440971878263 1105 12-13 1.4190669674348229 1105 14-15 1.4031619184965791 1105 16-17 1.3671932706890715 1105 18-19 1.4286084579485916 1105 20-21 1.622995232080811 1105 22-23 1.5637787814852544 1105 24-25 1.8150189920669746 1105 26-27 1.4706625380901457 1105 28-29 1.5375777580221168 1105 30-31 1.897556815960872 1105 32-33 1.8235853154144621 1105 34-35 1.909706169157932 1105 36-37 1.62728855904232 1105 38-39 1.8382559480487721 1105 40-41 2.0111765267254604 1105 42-43 1.8410093295464875 1105 44-45 1.7635888245232003 1105 46-47 1.6518414333869451 1105 48-49 1.7209615376433867 1105 50-51 1.8836259244384337 1105 52-53 1.7644165778592402 1105 54-55 1.9424499585983455 1105 56-57 1.8414320429232731 1105 58-59 1.7757782413691956 1105 60-61 1.619177080566196 1105 62-63 1.4846188260795579 1105 64-65 1.952581496034263 1105 66-67 2.1132990160953184 1105 68-69 2.061891082744534 1105 70-71 1.8899141487004307 1105 72-73 2.1244007446196633 1105 74-75 2.1249189640386845 1105 76-77 2.0472516479334786 1105 78-79 2.100411941234114 1105 80-81 2.0832909802543647 1105 82-83 2.0537370776377806 1105 84-85 2.270217652063792 1105 86-87 2.1966917161411175 1105 88-89 2.076080837092295 1105 90-91 2.053652726943369 1105 92-93 1.8267882963554243 1105 94-95 1.761263620831734 1105 96-97 1.856604149764209 1105 98-99 1.9656602601240998 1105 100-101 2.000654086628959 1105 102-103 1.7745859793433816 1105 104-105 1.4855759458222302 1105 106-107 1.7338103971842038 1105 108-109 1.6801442148071573 1105 110-111 1.8022945098018681 1105 112-113 1.7988700094353085 1105 114-115 1.6773871606761155 1105 116-117 1.7140192888810013 1105 118-119 1.7655741782424954 1105 120-121 1.7126367040818131 1105 122-123 1.9348482671567204 1105 124-125 1.7630749410931337 1106 1 0.2981264700808488 1106 2 0.1675268261153029 1106 3 0.3304177210239345 1106 4 0.09992597190659325 1106 5 0.24538786130876034 1106 6 0.32664628467593104 1106 7 0.5342687286228553 1106 8 0.21219991809388006 1106 9 0.8817354280632728 1106 10-11 0.5826787175587285 1106 12-13 0.6024902504769116 1106 14-15 0.6455907551179294 1106 16-17 0.5179101154924624 1106 18-19 0.6297680023877419 1106 20-21 0.33372788676478393 1106 22-23 0.43748864495521644 1106 24-25 0.3761984042261517 1106 26-27 0.37772307786014636 1106 28-29 0.39779648229455944 1106 30-31 0.7888928355017235 1106 32-33 0.546969938007642 1106 34-35 0.5749145170752961 1106 36-37 0.5616131840690883 1106 38-39 0.6881801465988602 1106 40-41 0.9180332497387376 1106 42-43 0.8519663367934811 1106 44-45 0.6861376723069732 1106 46-47 0.6128766745456353 1106 48-49 0.6155236796714689 1106 50-51 0.9392809861139497 1106 52-53 0.5877534928150503 1106 54-55 1.1322728580064663 1106 56-57 0.8101692483284708 1106 58-59 0.8108589920941576 1106 60-61 1.061316213232157 1106 62-63 1.0062806548188945 1106 64-65 1.1387450537550947 1106 66-67 1.2159321727799899 1106 68-69 1.143854984002953 1106 70-71 1.0647723358987697 1106 72-73 0.9030913930427928 1106 74-75 1.2469654356043591 1106 76-77 0.8742025060964664 1106 78-79 0.8045562956220493 1106 80-81 0.9548971005747973 1106 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1.1576891051443035 2204 60-61 1.3949308232351463 2204 62-63 1.2067161594030331 2204 64-65 1.5163433594526765 2204 66-67 1.8548533099797169 2204 68-69 1.8861626866086034 2204 70-71 1.5764898234916878 2204 72-73 1.774401349336813 2204 74-75 1.8995569892376807 2204 76-77 1.626107795926508 2204 78-79 1.6830141543383839 2204 80-81 1.5990110181102324 2204 82-83 1.4632681249339399 2204 84-85 1.7693570621796937 2204 86-87 1.5089166092451833 2204 88-89 1.4424263387676497 2204 90-91 1.5017627508437421 2204 92-93 1.2993565782359262 2204 94-95 1.2396065502524145 2204 96-97 1.3296946665210925 2204 98-99 1.3723090134065536 2204 100-101 1.4327873401510907 2204 102-103 1.3179476380865331 2204 104-105 1.3369028528751414 2204 106-107 1.409676789200379 2204 108-109 1.3941107678156612 2204 110-111 1.2782741702122031 2204 112-113 1.3053003881779688 2204 114-115 1.2348370667386064 2204 116-117 1.2179443903918035 2204 118-119 1.089094184607216 2204 120-121 1.123178345506556 2204 122-123 0.43301783706755614 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1.7141118745483617 2205 60-61 1.7373709719365564 2205 62-63 1.3518444961282832 2205 64-65 1.8739782427137186 2205 66-67 2.051701434042876 2205 68-69 2.1169496703512642 2205 70-71 1.8164647635901154 2205 72-73 1.915301941601534 2205 74-75 2.059875106466592 2205 76-77 1.8090709909255978 2205 78-79 1.831225965851388 2205 80-81 1.6858251034118048 2205 82-83 1.4490048701519989 2205 84-85 1.986526108229608 2205 86-87 1.6543153670728117 2205 88-89 1.6464787741375915 2205 90-91 1.7786983195643842 2205 92-93 1.4729181768798867 2205 94-95 1.6080103493156734 2205 96-97 1.5613481703489924 2205 98-99 1.4365096213659605 2205 100-101 1.4577824872282186 2205 102-103 1.206321148813764 2205 104-105 1.34615922796835 2205 106-107 1.4451820392978334 2205 108-109 1.545403621059343 2205 110-111 1.5927096854349045 2205 112-113 1.5757751643821543 2205 114-115 1.3894683674257067 2205 116-117 1.3064002322527095 2205 118-119 1.2702259032231673 2205 120-121 1.1132823695425813 2205 122-123 -2.1784368431991403 2205 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-0.07832250575981803 2207 122-123 -2.201842454406286 2207 124-125 -0.8033913436301034 2208 1 -0.13586048590272526 2208 2 0.11347796287314083 2208 3 6.526690042321093E-4 2208 4 0.23337471852107683 2208 5 0.39193561179069647 2208 6 0.04342170805093204 2208 7 0.5879580803616697 2208 8 0.17913583464512328 2208 9 0.6682838596653511 2208 10-11 0.3681041212129301 2208 12-13 0.3647772561754721 2208 14-15 0.13188759655339055 2208 16-17 0.3402563322547607 2208 18-19 0.5956819275835485 2208 20-21 0.20159988528215678 2208 22-23 0.38278332671936255 2208 24-25 0.7239174211231401 2208 26-27 0.3751272407487818 2208 28-29 0.3578389991262583 2208 30-31 0.8293314870558213 2208 32-33 0.7781104906563101 2208 34-35 0.7630782556419256 2208 36-37 0.4807520886785568 2208 38-39 0.47405574577527076 2208 40-41 0.6600193112147004 2208 42-43 0.5262592673321222 2208 44-45 0.615875688835338 2208 46-47 0.5721172063533793 2208 48-49 0.8119515439570364 2208 50-51 0.9427784305557019 2208 52-53 0.46978144770544006 2208 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2216 98-99 -3.9332336411642608 2216 100-101 -4.310347448878918 2216 102-103 -4.281997886777905 2216 104-105 -4.160609708521818 2216 106-107 -4.287974991722805 2216 108-109 -4.227117704691167 2216 110-111 -3.9132030477422006 2216 112-113 -3.977948438855325 2216 114-115 -3.729981418787016 2216 116-117 -3.665135413459616 2216 118-119 -3.923302374274236 2216 120-121 -3.8174787031453086 2216 122-123 -3.865085331939863 2216 124-125 -3.844491711494488 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 126018.0 3 42252.0 4 20212.0 5 17015.0 6 18362.0 7 21438.0 8 24020.0 9 25289.0 10 27118.0 11 29493.0 12 32525.0 13 35891.0 14 37887.0 15 30443.0 16 20857.0 17 18170.0 18 15941.0 19 14984.0 20 14459.0 21 15181.0 22 18676.0 23 25093.0 24 34005.0 25 44879.0 26 55515.0 27 62713.0 28 70771.0 29 81526.0 30 90577.0 31 103263.0 32 120968.0 33 142922.0 34 174335.0 35 221580.0 36 304676.0 37 380254.0 38 138965.0 39 629.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97824531046045 20.28499991152345 14.433913852453491 23.302840925562606 2 15.858889014254016 21.35292790251608 38.53830531422769 24.249877769002218 3 19.060499409154605 25.846496034829414 29.822686206561954 25.270318349454023 4 12.664125833416543 17.563136189541385 36.90650097871691 32.866236998325164 5 12.777642801427055 39.28681839345715 33.714254981943675 14.22128382317212 6 30.945877181402665 37.84652056849137 17.12021939924713 14.087382850858834 7 27.15554363272636 33.776313259711955 20.764096164055452 18.304046943506233 8 26.2973964440961 35.63320498461395 19.229441325780343 18.839957245509613 9 26.151659594825233 15.922136280314206 20.180021678121268 37.7461824467393 10-11 24.975967342665076 27.317064089342814 26.70881972197337 20.99814884601874 12-13 25.879222260166294 24.730826918361238 27.711532357184442 21.678418464288026 14-15 22.6609103734006 25.776067204059444 26.260348785036747 25.302673637503208 16-17 21.80911101741738 29.22749184682755 26.791018139515817 22.172378996239253 18-19 21.585299829418958 26.87547471118661 30.11491607190844 21.424309387485998 20-21 22.484118391862236 26.031658094237116 30.32488727588931 21.15933623801134 22-23 22.0057419528695 25.898118040815742 30.254416239492876 21.84172376682188 24-25 21.668569171893072 26.210472868144162 30.34801741403466 21.77294054592811 26-27 21.62244195053499 26.465343144067305 30.260285840712793 21.651929064684914 28-29 21.403431306914516 26.432312626842364 30.445063434345865 21.71919263189725 30-31 21.577045793589885 26.111366962700224 30.39791430703908 21.913672936670807 32-33 21.43365677535823 26.29117304148332 30.261198239873632 22.013971943284815 34-35 21.8308252783658 26.38981686368985 30.081972900858318 21.697384957086037 36-37 22.075123309031643 26.15206430855832 29.914078112550506 21.85873426985953 38-39 21.821479512539575 26.234686940848267 29.93643006037311 22.00740348623905 40-41 21.883580403831495 26.214539972352775 29.911843681672927 21.990035942142796 42-43 21.914734255139063 26.409472726346156 29.52970155992637 22.146091458588415 44-45 21.987233310359937 26.50012364263424 29.38012258955629 22.13252045744954 46-47 22.186183839254937 26.837272722657 28.635069437719114 22.34147400036895 48-49 22.333118081493065 26.981862055037027 28.186062587512662 22.498957275957245 50-51 22.354036071719026 27.059696435589213 27.868827786710643 22.717439705981118 52-53 22.622920732024934 27.115532992327463 27.82657931470494 22.434966960942663 54-55 22.3850721243834 26.985614290653242 27.784514893754842 22.844798691208513 56-57 22.331910748554144 27.342888063357208 27.58669257971129 22.738508608377362 58-59 22.376876762241242 26.925071674349667 27.779165151316278 22.918886412092814 60-61 22.411826385477916 26.931737235896623 28.02183006368794 22.634606314937518 62-63 22.35455021218509 26.904903434019158 28.069077166470187 22.671469187325574 64-65 22.13730159252837 26.78501572582959 28.033278734331795 23.044403947310236 66-67 22.13167716257424 27.082169045966864 27.802796697414827 22.98335709404407 68-69 22.16202086988572 26.85627836042206 28.086883263660482 22.894817506031732 70-71 22.11451577324766 26.839165397150577 28.132501561312033 22.91381726828973 72-73 22.3383617749367 26.899976571783963 27.681124102741666 23.08053755053767 74-75 22.156344004651764 27.158416385271728 27.58804984846422 23.097189761612288 76-77 22.21982979904523 26.98076654742997 27.658990310498083 23.140413343026722 78-79 22.372330526865294 27.0126497917527 27.6015868466838 23.013432834698204 80-81 22.102097223021424 27.238080301719098 27.657107439663893 23.002715035595585 82-83 22.371064113379262 27.20765249074899 27.481092738009227 22.940190657862523 84-85 22.234535157745565 26.943903912216392 27.320619564015523 23.500941366022516 86-87 22.187209759544803 26.831734015772348 27.547256952090425 23.433799272592427 88-89 22.194171427152995 26.89419967226642 27.50336891615865 23.408259984421935 90-91 22.229234336652826 27.11318875297798 27.478058987385907 23.17951792298329 92-93 22.203076453236577 26.971300653179338 27.54102257336937 23.28460032021471 94-95 22.137164989593042 26.808881306515197 27.517058460251587 23.53689524364017 96-97 22.17786286917173 26.75606152381085 27.505485943966928 23.56058966305049 98-99 21.907501667981748 26.584187758706413 27.483713201915677 24.024597371396165 100-101 21.868537539789056 26.76333195492589 27.54178426851603 23.826346236769027 102-103 22.13760153088252 26.451915361422955 27.574408341023037 23.836074766671487 104-105 21.90158998321108 26.522003869275068 27.627124189889336 23.949281957624518 106-107 21.76372626414166 26.556367880088715 27.69962303946107 23.980282816308556 108-109 22.00346233144358 26.52303469627688 27.263979642724706 24.20952332955483 110-111 21.783013448243118 26.658116757039334 27.447504087690483 24.111365707027065 112-113 21.86675763163625 26.685500276618257 27.495440782068066 23.952301309677427 114-115 21.620953797579638 26.712406175868004 27.31820237523259 24.348437651319767 116-117 21.653957159759933 26.64065843720453 27.42506117186718 24.280323231168353 118-119 21.541588085307716 26.632964360591732 27.313554619192338 24.511892934908214 120-121 21.605351057359997 26.684485220805023 27.309889767676793 24.400273954158187 122-123 21.64530266118439 26.61408172072937 27.201454751967237 24.539160866119005 124-125 21.514554496000432 26.561395709046238 27.14123602728138 24.782813767671946 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 413.0 1 340.0 2 404.0 3 609.0 4 513.5 5 412.0 6 840.0 7 1879.5 8 3067.0 9 3681.0 10 3039.5 11 2101.0 12 1940.0 13 2018.0 14 2189.5 15 2462.0 16 2770.5 17 3147.0 18 3568.5 19 3959.0 20 4478.5 21 5227.0 22 6261.5 23 7512.0 24 9148.5 25 11622.5 26 15209.0 27 19552.0 28 24041.0 29 29210.5 30 35024.5 31 41158.0 32 47231.5 33 52313.5 34 57394.0 35 62445.0 36 68067.0 37 73784.0 38 76730.0 39 78586.5 40 80643.5 41 82511.0 42 84566.5 43 87404.0 44 90031.0 45 91574.5 46 92257.0 47 92597.5 48 93848.5 49 93224.0 50 90596.5 51 88256.0 52 86039.0 53 85099.0 54 84231.0 55 79543.0 56 72318.0 57 65953.0 58 61797.0 59 56380.0 60 48225.0 61 40310.5 62 33551.0 63 26538.5 64 20568.5 65 16577.5 66 12661.5 67 9913.0 68 7228.0 69 4693.0 70 3437.5 71 2709.0 72 2223.5 73 2036.5 74 1661.5 75 1226.0 76 863.0 77 517.5 78 337.0 79 193.0 80 130.5 81 101.0 82 64.5 83 40.0 84 22.0 85 20.5 86 15.5 87 7.5 88 4.0 89 2.5 90 2.5 91 1.0 92 2.0 93 1.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10647252136408186 2 7.521901897851067E-5 3 0.0 4 0.1849259581586685 5 0.0 6 1.880475474462767E-4 7 0.04020456564401396 8 0.0 9 0.0 10-11 7.521901897851068E-4 12-13 6.769711708065961E-4 14-15 5.6414264233883E-4 16-17 0.004550750648199897 18-19 0.0018240612102288839 20-21 0.0032908320803098426 22-23 0.0018992802292073945 24-25 0.004776407705135428 26-27 0.004268679327030481 28-29 0.00338485585403298 30-31 9.590424919760112E-4 32-33 7.521901897851067E-5 34-35 0.0013351375868685645 36-37 0.0012599185678900538 38-39 7.333854350404791E-4 40-41 0.001711232681761118 42-43 9.966520014652665E-4 44-45 0.0011094805299330325 46-47 3.196808306586704E-4 48-49 3.7609509489255335E-5 50-51 0.04187818881628582 52-53 1.316332832123937E-4 54-55 0.1364473004270184 56-57 3.5729034014792574E-4 58-59 3.7609509489255335E-5 60-61 0.0 62-63 7.709949445297345E-4 64-65 0.062036885902526685 66-67 0.03018163136512741 68-69 0.0017676469459950008 70-71 0.003027565513885055 72-73 0.06955878780037776 74-75 0.0046635791766676616 76-77 0.00169242792701649 78-79 0.0016548184175272347 80-81 1.880475474462767E-4 82-83 1.12828528467766E-4 84-85 0.0 86-87 2.82071321169415E-4 88-89 0.001316332832123937 90-91 4.3250935912643635E-4 92-93 0.0022941800788445757 94-95 0.003911388986882556 96-97 0.0023129848335892034 98-99 0.0 100-101 0.0013727470963578198 102-103 0.0016360136627826074 104-105 2.25657056935532E-4 106-107 1.12828528467766E-4 108-109 0.0 110-111 1.6924279270164904E-4 112-113 1.12828528467766E-4 114-115 1.6924279270164904E-4 116-117 0.0 118-119 0.0012599185678900538 120-121 0.0023693990978230863 122-123 0.0011094805299330325 124-125 0.008988672767932025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2658902.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.24397340653104 #Duplication Level Percentage of deduplicated Percentage of total 1 78.07906261497247 43.13397658708411 2 11.366013907922582 12.55807540135074 3 4.245927444208053 7.036857084416699 4 2.033653677014285 4.493884386042849 5 1.144103352818713 3.1602407598719995 6 0.6828345150560832 2.263349507449187 7 0.46599091382848606 1.802023275585819 8 0.32608369981213714 1.4411327392579962 9 0.24613305904635985 1.2237631351582714 >10 1.2644852006840566 12.558591273869396 >50 0.09488162209455128 3.6034290773604454 >100 0.04784285918025222 4.630520433319848 >500 0.002500855838009161 0.9388550191167047 >1k 2.7787287088765645E-4 0.2783867850159657 >5k 2.084046531657423E-4 0.8769145350999196 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9238 0.34743664866174084 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8539 0.3211476015287513 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5073 0.19079304163899233 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.760950948925534E-5 2 0.0 0.0 0.0 0.0 3.760950948925534E-5 3 0.0 0.0 0.0 0.0 3.760950948925534E-5 4 0.0 0.0 0.0 3.760950948925534E-5 7.521901897851068E-5 5 0.0 0.0 0.0 3.760950948925534E-5 7.521901897851068E-5 6 0.0 0.0 0.0 3.760950948925534E-5 7.521901897851068E-5 7 0.0 0.0 0.0 3.760950948925534E-5 7.521901897851068E-5 8 0.0 0.0 0.0 3.760950948925534E-5 7.521901897851068E-5 9 0.0 0.0 0.0 7.521901897851068E-5 1.1282852846776602E-4 10-11 0.0 0.0 0.0 7.521901897851068E-5 1.1282852846776602E-4 12-13 3.760950948925534E-5 0.0 0.0 7.521901897851068E-5 2.0685230219090437E-4 14-15 3.760950948925534E-5 0.0 0.0 9.402377372313835E-5 2.4446181168015973E-4 16-17 3.760950948925534E-5 0.0 0.0 1.5043803795702137E-4 3.3848558540329807E-4 18-19 5.6414264233883016E-5 0.0 0.0 2.2565705693553204E-4 3.3848558540329807E-4 20-21 7.521901897851068E-5 0.0 0.0 2.8207132116941507E-4 3.3848558540329807E-4 22-23 1.1282852846776602E-4 0.0 0.0 4.701188686156918E-4 4.889236233603195E-4 24-25 1.5043803795702137E-4 0.0 0.0 9.966520014652665E-4 6.393616613173408E-4 26-27 1.5043803795702137E-4 0.0 0.0 0.0015231851343148412 6.393616613173408E-4 28-29 3.0087607591404274E-4 0.0 0.0 0.0025386418905247355 6.581664160619685E-4 30-31 3.0087607591404274E-4 0.0 0.0 0.005679035932877556 6.769711708065961E-4 32-33 3.0087607591404274E-4 1.880475474462767E-5 0.0 0.011583728922690644 7.145806802958515E-4 34-35 3.3848558540329807E-4 3.760950948925534E-5 0.0 0.019970649538794585 7.521901897851068E-4 36-37 3.760950948925534E-4 3.760950948925534E-5 0.0 0.034412701182668634 7.521901897851068E-4 38-39 3.760950948925534E-4 3.760950948925534E-5 0.0 0.058934101369663115 7.521901897851068E-4 40-41 3.760950948925534E-4 3.760950948925534E-5 0.0 0.08870202813040871 7.521901897851068E-4 42-43 4.701188686156918E-4 3.760950948925534E-5 0.0 0.11875202621232372 7.521901897851068E-4 44-45 5.265331328495748E-4 3.760950948925534E-5 0.0 0.15308950837601387 7.521901897851068E-4 46-47 6.205569065727131E-4 3.760950948925534E-5 0.0 0.1951745494944906 7.897996992743621E-4 48-49 6.769711708065961E-4 3.760950948925534E-5 0.0 0.24231806963927216 7.897996992743621E-4 50-51 6.769711708065961E-4 3.760950948925534E-5 0.0 0.29907081945855846 7.897996992743621E-4 52-53 6.769711708065961E-4 3.760950948925534E-5 0.0 0.35332253689680926 8.650187182528728E-4 54-55 6.769711708065961E-4 3.760950948925534E-5 0.0 0.41278317139932197 9.590424919760112E-4 56-57 6.769711708065961E-4 3.760950948925534E-5 0.0 0.48149574523619143 0.0010154567562098943 58-59 6.769711708065961E-4 3.760950948925534E-5 0.0 0.5619612907884532 0.0010718710204437772 60-61 6.769711708065961E-4 3.760950948925534E-5 0.0 0.6538037129612149 0.001090675775188405 62-63 6.769711708065961E-4 3.760950948925534E-5 0.0 0.7459470112098905 0.001090675775188405 64-65 6.769711708065961E-4 3.760950948925534E-5 0.0 0.8377142143636734 0.0011658947941669156 66-67 7.521901897851068E-4 3.760950948925534E-5 0.0 0.9425507220649727 0.0011658947941669156 68-69 7.897996992743621E-4 3.760950948925534E-5 0.0 1.0679972409663838 0.0011846995489115434 70-71 7.897996992743621E-4 3.760950948925534E-5 0.0 1.2124741716693583 0.001259918567890054 72-73 8.086044540189898E-4 3.760950948925534E-5 0.0 1.3646798565723746 0.0014479661153363305 74-75 8.274092087636175E-4 3.760950948925534E-5 0.0 1.5136135141498257 0.0015043803795702136 76-77 8.274092087636175E-4 3.760950948925534E-5 0.0 1.6813707312266493 0.0015043803795702136 78-79 8.274092087636175E-4 3.760950948925534E-5 0.0 1.8808515695576595 0.0015043803795702136 80-81 8.650187182528728E-4 3.760950948925534E-5 0.0 2.106734283550127 0.0015043803795702136 82-83 8.650187182528728E-4 3.760950948925534E-5 0.0 2.3416056703105266 0.0015043803795702136 84-85 8.650187182528728E-4 3.760950948925534E-5 0.0 2.5769095664300528 0.0015043803795702136 86-87 9.026282277421282E-4 3.760950948925534E-5 0.0 2.828949694272297 0.001541989889059469 88-89 9.026282277421282E-4 3.760950948925534E-5 0.0 3.1100431681949914 0.0015984041532933519 90-91 9.966520014652665E-4 3.760950948925534E-5 0.0 3.4348388921442012 0.0016172089080379796 92-93 0.0010530662656991496 3.760950948925534E-5 0.0 3.786487805868738 0.001654818417527235 94-95 0.0010530662656991496 3.760950948925534E-5 0.0 4.136500705930493 0.0017488421912503732 96-97 0.0010530662656991496 3.760950948925534E-5 0.0 4.487848743579116 0.0018240612102288839 98-99 0.0011658947941669156 3.760950948925534E-5 0.0 4.8792509088337965 0.0019180849839520223 100-101 0.0012223090584007985 3.760950948925534E-5 0.0 5.336394496675695 0.0019556944934412774 102-103 0.001316332832123937 3.760950948925534E-5 0.0 5.824885610676889 0.0019556944934412774 104-105 0.0015795993985487243 3.760950948925534E-5 0.0 6.305027413571467 0.0019556944934412774 106-107 0.0015984041532933519 3.760950948925534E-5 0.0 6.7890994102076725 0.0019556944934412774 108-109 0.0016172089080379796 3.760950948925534E-5 0.0 7.301228100922862 0.001974499248185905 110-111 0.0016172089080379796 3.760950948925534E-5 0.0 7.87734937203402 0.0020309135124197885 112-113 0.0016360136627826074 3.760950948925534E-5 0.0 8.4819222370738 0.0020685230219090437 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6675 0.0 66.05085 1 GTATCAA 11685 0.0 54.120575 1 ATCAACG 15390 0.0 39.11195 3 TATCAAC 16205 0.0 38.579197 2 TCAACGC 15855 0.0 37.73933 4 CAACGCA 15825 0.0 37.63194 5 AACGCAG 16200 0.0 36.430313 6 GTACATG 13645 0.0 32.18026 1 GTGGTAT 2910 0.0 31.57279 1 CGCAGAG 19320 0.0 31.059544 8 ACGCAGA 19245 0.0 30.511635 7 TACATGG 14020 0.0 30.478798 2 TCACCGC 620 0.0 28.837412 4 GCAGAGT 20410 0.0 28.206121 9 TGGTATC 3195 0.0 27.793427 2 ACATGGG 14285 0.0 27.451895 3 GAGTACT 12695 0.0 26.142384 12-13 AGTACTT 13360 0.0 25.531166 12-13 ACCGCAG 725 0.0 25.438408 6 CACCGCA 705 0.0 25.316193 5 >>END_MODULE