FastQCFastQC Report
Thu 26 May 2016
SRR1364510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537732
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26780.10552729760274135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68600.055.885931
GGTATCA36400.050.535651
ATCAACG79650.045.9462623
CAACGCA83050.044.407035
TCAACGC82100.044.3420684
TATCAAC88700.042.8659482
AACGCAG84050.042.5766756
CGCAGAG97450.035.6293078
ACGCAGA99650.035.435467
GTACATG110900.034.7306941
TACATGG110200.033.638892
GCAGAGT104850.031.240289
ACATGGG113400.030.4876483
CGCATAG3250.029.319558
GAGTACT49300.024.9788812-13
AGAGTAC102100.024.106310-11
CAGAGTA106500.024.00459110-11
CATGGGA70900.023.0261944
AACGCAT4600.022.0052386
AGTACTT55550.021.90074212-13
Produced by FastQC (version 0.11.5)