##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364510_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2537732 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.729827657136372 30.0 26.0 31.0 7.0 34.0 2 25.89848337019039 30.0 26.0 31.0 7.0 34.0 3 25.987374159288688 30.0 26.0 31.0 7.0 34.0 4 28.737516806345194 35.0 27.0 37.0 7.0 37.0 5 29.364141288362994 35.0 28.0 37.0 2.0 37.0 6 29.411726297339513 35.0 29.0 37.0 2.0 37.0 7 29.244698021698113 35.0 29.0 37.0 2.0 37.0 8 28.919246003912153 35.0 28.0 37.0 2.0 37.0 9 29.543032518800253 35.0 27.0 39.0 2.0 39.0 10-11 29.589083677866693 35.0 27.0 39.0 2.0 39.0 12-13 29.43264615806555 35.0 27.0 39.0 2.0 39.0 14-15 30.0487433267185 36.0 27.0 40.0 2.0 41.0 16-17 30.286340913855362 36.0 27.0 40.0 2.0 41.0 18-19 30.233358959890168 36.0 27.0 40.0 2.0 41.0 20-21 30.0267230739889 36.0 27.0 40.0 2.0 41.0 22-23 30.067442897831608 36.0 27.0 40.0 2.0 41.0 24-25 30.092864809995696 36.0 27.0 40.0 2.0 41.0 26-27 30.198208282040817 36.0 27.0 40.0 2.0 41.0 28-29 30.12409801350182 36.0 27.0 40.0 2.0 41.0 30-31 29.769062887649287 36.0 27.0 40.0 2.0 41.0 32-33 29.778323124742883 36.0 27.0 40.0 2.0 41.0 34-35 29.48101907530031 35.5 25.5 40.0 2.0 41.0 36-37 29.241821240383146 35.0 25.0 40.0 2.0 41.0 38-39 29.094560024462787 35.0 25.0 40.0 2.0 41.0 40-41 29.146874650278278 35.0 25.0 40.0 2.0 41.0 42-43 28.990711982195123 35.0 25.0 40.0 2.0 41.0 44-45 28.648974950861636 35.0 24.0 40.0 2.0 41.0 46-47 28.386863151822176 35.0 23.5 39.0 2.0 41.0 48-49 28.139539754394868 34.0 22.5 39.0 2.0 41.0 50-51 28.128617206229816 34.0 23.0 39.0 2.0 41.0 52-53 27.93315763839523 34.0 22.0 39.0 2.0 40.5 54-55 27.818378378804383 34.0 21.5 39.0 2.0 40.0 56-57 27.413989735716775 34.0 20.0 39.0 2.0 40.0 58-59 27.197713548948432 33.0 18.5 39.0 2.0 40.0 60-61 26.79061126233976 33.0 16.0 38.0 2.0 40.0 62-63 26.74482490664893 33.0 18.0 38.0 2.0 40.0 64-65 26.573018151640916 33.0 16.0 38.0 2.0 40.0 66-67 26.350521646887852 33.0 16.5 37.0 2.0 40.0 68-69 26.182207971527333 32.5 15.0 37.0 2.0 40.0 70-71 26.05640765061086 32.5 13.5 36.5 2.0 39.0 72-73 26.023805705251775 33.0 14.5 36.0 2.0 39.0 74-75 25.746493325536346 32.0 13.0 36.0 2.0 39.0 76-77 25.48239491010083 32.0 11.0 35.5 2.0 39.0 78-79 25.181537885009135 32.0 9.0 35.0 2.0 37.5 80-81 25.166572356734278 32.0 9.0 35.0 2.0 37.0 82-83 24.964124659341493 32.0 7.0 35.0 2.0 37.0 84-85 24.775160458235938 32.0 7.0 35.0 2.0 36.0 86-87 24.56210643992352 32.0 6.0 35.0 2.0 36.0 88-89 24.349304418275846 32.0 2.0 35.0 2.0 36.0 90-91 24.18241681942774 32.0 2.0 35.0 2.0 35.0 92-93 24.076854057087196 32.0 2.0 35.0 2.0 35.0 94-95 23.997417773035135 32.0 2.0 35.0 2.0 35.0 96-97 23.879565493913464 32.0 2.0 35.0 2.0 35.0 98-99 23.771890609410292 32.0 2.0 35.0 2.0 35.0 100-101 23.723117334690976 32.0 2.0 35.0 2.0 35.0 102-103 22.622339356559323 29.5 2.0 34.0 2.0 35.0 104-105 23.365592190191872 31.0 2.0 34.5 2.0 35.0 106-107 23.459785351644697 32.0 2.0 35.0 2.0 35.0 108-109 23.384824520477338 32.0 2.0 35.0 2.0 35.0 110-111 23.275442599927807 32.0 2.0 35.0 2.0 35.0 112-113 23.251942679526444 32.0 2.0 35.0 2.0 35.0 114-115 23.201543149552435 32.0 2.0 35.0 2.0 35.0 116-117 23.025698340092653 32.0 2.0 35.0 2.0 35.0 118-119 22.865563424348984 32.0 2.0 35.0 2.0 35.0 120-121 22.712850293096356 32.0 2.0 35.0 2.0 35.0 122-123 22.436251739742417 31.0 2.0 35.0 2.0 35.0 124-125 21.46832486645556 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6421862720071019 1101 2 1.1870551550832076 1101 3 1.5044396232846857 1101 4 1.9773666023802754 1101 5 1.4425648178866872 1101 6 1.4273534042739513 1101 7 1.3138830997677715 1101 8 1.7320116915977444 1101 9 2.1512464723702216 1101 10-11 2.0992294839041925 1101 12-13 1.8953731023900637 1101 14-15 2.2763990793543414 1101 16-17 1.8341774663210337 1101 18-19 1.884233415239489 1101 20-21 2.0801219471338754 1101 22-23 1.973217006978441 1101 24-25 1.9823702420458211 1101 26-27 1.6643246245633208 1101 28-29 1.588583317702689 1101 30-31 1.7368466380040033 1101 32-33 1.8825270826179796 1101 34-35 2.1147051628707842 1101 36-37 2.249716825690154 1101 38-39 2.285553170585512 1101 40-41 2.0024215575685 1101 42-43 1.9273844480652542 1101 44-45 2.040918806873229 1101 46-47 2.1110818027847778 1101 48-49 2.138227419044579 1101 50-51 2.076783556061116 1101 52-53 1.940071809673917 1101 54-55 1.9136327896255771 1101 56-57 2.2584644245226784 1101 58-59 2.247377144186146 1101 60-61 2.3590047921842263 1101 62-63 2.1452734560827125 1101 64-65 2.0971473288183624 1101 66-67 2.044292005229334 1101 68-69 1.9092156986613276 1101 70-71 1.9483687084366288 1101 72-73 1.7443027546582641 1101 74-75 1.7831351662816424 1101 76-77 1.9416885953552914 1101 78-79 1.9611036518888518 1101 80-81 1.8621830029966127 1101 82-83 1.8291154345283722 1101 84-85 1.7712661548720163 1101 86-87 1.8100662410448507 1101 88-89 1.9110863790237573 1101 90-91 1.8809616592595582 1101 92-93 1.7721148864250331 1101 94-95 1.7609570584945011 1101 96-97 1.714037223476435 1101 98-99 1.7118069526036521 1101 100-101 1.6505171375238952 1101 102-103 1.6577263804882953 1101 104-105 1.5958770954747692 1101 106-107 1.732463415868338 1101 108-109 1.7301757972478384 1101 110-111 1.847186359468843 1101 112-113 1.8114197806575767 1101 114-115 1.7032782652977794 1101 116-117 1.8820161855180864 1101 118-119 1.8980928277135583 1101 120-121 1.801512492217416 1101 122-123 1.8602510691644802 1101 124-125 1.9019452876177887 1102 1 0.8857403210687593 1102 2 0.5245510984184882 1102 3 0.6053096120280799 1102 4 1.190912813659537 1102 5 1.000338931058959 1102 6 0.961936726126158 1102 7 0.7752145379498145 1102 8 1.1331319561433126 1102 9 1.4707474719936435 1102 10-11 1.0251637797131217 1102 12-13 1.4110349855091329 1102 14-15 1.6277668387180917 1102 16-17 1.1206084203567244 1102 18-19 1.2810829877333667 1102 20-21 1.3759205755729838 1102 22-23 1.2447451717127045 1102 24-25 0.9580301899572241 1102 26-27 0.9399289719301649 1102 28-29 0.6698943781607696 1102 30-31 0.6007213306925152 1102 32-33 0.4930771918672754 1102 34-35 1.0167890447468295 1102 36-37 1.2345091486945527 1102 38-39 1.171607868968664 1102 40-41 0.720566693550964 1102 42-43 0.728400578792936 1102 44-45 1.2366072101189687 1102 46-47 0.9778088537987379 1102 48-49 1.134199871739785 1102 50-51 0.9879675383077213 1102 52-53 1.0998678512876445 1102 54-55 0.850768008721186 1102 56-57 0.922850237830378 1102 58-59 1.0079835683612153 1102 60-61 1.4649251052566896 1102 62-63 1.0834040569589547 1102 64-65 1.3126868550203312 1102 66-67 1.2470894263642016 1102 68-69 1.0886026562546363 1102 70-71 1.2699039404421342 1102 72-73 1.1045877136680886 1102 74-75 1.2130255074905207 1102 76-77 1.083809538307868 1102 78-79 1.0057303124424557 1102 80-81 0.6775993367106103 1102 82-83 0.5849125423560544 1102 84-85 0.6736297935318802 1102 86-87 0.8290866088185034 1102 88-89 0.6673330367402457 1102 90-91 0.6654214314331846 1102 92-93 0.5976299552760729 1102 94-95 0.7402918917732357 1102 96-97 0.8227656606450715 1102 98-99 0.9205947790886242 1102 100-101 0.8037086929767128 1102 102-103 0.5746996889101332 1102 104-105 0.6446209199043622 1102 106-107 0.979818674581491 1102 108-109 1.1370881625742228 1102 110-111 0.8264715049831892 1102 112-113 0.6829945779008817 1102 114-115 0.7305893140195998 1102 116-117 0.913299767813271 1102 118-119 1.000071159766108 1102 120-121 1.018416231390713 1102 122-123 1.1216161725609517 1102 124-125 1.054557301315537 1103 1 1.4145791488406978 1103 2 1.1803628691936439 1103 3 1.2888052320502261 1103 4 1.9795297409842192 1103 5 1.4583251050497665 1103 6 1.4296441860129256 1103 7 1.4362846155664002 1103 8 1.6808821226529815 1103 9 2.4165141443924014 1103 10-11 1.9800350625401286 1103 12-13 2.1308462944196975 1103 14-15 2.2748479483293877 1103 16-17 2.175016183638565 1103 18-19 1.8743794137485388 1103 20-21 2.3362262495325936 1103 22-23 2.06001312546233 1103 24-25 2.1636773659887645 1103 26-27 1.9308311144644676 1103 28-29 1.7830619513096195 1103 30-31 1.9626810746658307 1103 32-33 1.9677553260154816 1103 34-35 1.8194280074004716 1103 36-37 2.2089086359512997 1103 38-39 2.2264891005264147 1103 40-41 2.321441306824969 1103 42-43 2.322972363609182 1103 44-45 2.249313939544436 1103 46-47 2.4922724948338235 1103 48-49 2.557795014577941 1103 50-51 2.3225295937852444 1103 52-53 2.338303830919944 1103 54-55 2.170104733373293 1103 56-57 2.509123994942861 1103 58-59 2.5477575983821588 1103 60-61 2.521494260412247 1103 62-63 2.3387598220486865 1103 64-65 2.3178976374914946 1103 66-67 2.359774146838472 1103 68-69 2.321315928127767 1103 70-71 2.423590652184348 1103 72-73 2.341822109822619 1103 74-75 2.412975656043013 1103 76-77 2.4682463754713027 1103 78-79 2.5548580199048807 1103 80-81 2.0591412137019383 1103 82-83 2.164556982708561 1103 84-85 2.1113096506049516 1103 86-87 2.1404942774954634 1103 88-89 2.3148267111482816 1103 90-91 2.25876883756003 1103 92-93 2.117350254542014 1103 94-95 1.8636042218078224 1103 96-97 2.049724108556841 1103 98-99 2.009258894720645 1103 100-101 1.8119548845594657 1103 102-103 1.9743752677119453 1103 104-105 2.1283387345873415 1103 106-107 1.8120506092336122 1103 108-109 2.217852168028333 1103 110-111 2.3273456173332114 1103 112-113 2.175990899723871 1103 114-115 2.040706532767903 1103 116-117 2.189952094104246 1103 118-119 2.1542223675382175 1103 120-121 2.1761776109886277 1103 122-123 2.1891825747904825 1103 124-125 2.105953718859393 1104 1 1.390322890398899 1104 2 1.2264833835293523 1104 3 1.1960591344630451 1104 4 1.5400171396826465 1104 5 1.167992498222656 1104 6 1.0178456226533967 1104 7 1.1680915823517637 1104 8 1.5151173533442872 1104 9 1.952110710883069 1104 10-11 1.7307677242389268 1104 12-13 1.2431987068552317 1104 14-15 1.6422114789961384 1104 16-17 1.361592142550034 1104 18-19 0.6352707716500667 1104 20-21 -3.894557389112421 1104 22-23 -0.523153210567262 1104 24-25 0.8971810861354932 1104 26-27 1.1562506142495863 1104 28-29 0.799236147803974 1104 30-31 1.5334031937105266 1104 32-33 1.4124584337481672 1104 34-35 1.6270984357833314 1104 36-37 1.652465385935951 1104 38-39 1.6551777344133285 1104 40-41 1.7797889496448356 1104 42-43 1.6289635457678244 1104 44-45 1.721502293317151 1104 46-47 1.7074970850229185 1104 48-49 1.7989838882886495 1104 50-51 1.6855129826297919 1104 52-53 1.682308523791999 1104 54-55 1.590807474655218 1104 56-57 1.998911578209686 1104 58-59 1.9528943363180638 1104 60-61 2.0889399067469725 1104 62-63 1.7815656837259937 1104 64-65 1.8582045831723626 1104 66-67 1.8190201696006305 1104 68-69 1.8344218189676482 1104 70-71 1.9926088231537733 1104 72-73 1.9298978787936392 1104 74-75 1.9783062963149938 1104 76-77 1.9819196894140845 1104 78-79 1.913626985170719 1104 80-81 1.6503977656880657 1104 82-83 1.6308332002460126 1104 84-85 1.562724706934766 1104 86-87 1.481088196331413 1104 88-89 1.6206960628182223 1104 90-91 1.5957716348647928 1104 92-93 1.2783641144388689 1104 94-95 1.4313570824260715 1104 96-97 1.6077955223277165 1104 98-99 1.4945092515784566 1104 100-101 1.1212810564344657 1104 102-103 1.4762782695384438 1104 104-105 1.5993848076165662 1104 106-107 1.7762031870516388 1104 108-109 1.744857526651245 1104 110-111 1.7846660948319837 1104 112-113 1.727695950988604 1104 114-115 1.6123397424161077 1104 116-117 1.8244795740741644 1104 118-119 1.8409441393180543 1104 120-121 1.783756810399236 1104 122-123 1.6551285549599335 1104 124-125 1.7896270903598825 1105 1 1.4289965378768343 1105 2 1.0717779887863763 1105 3 1.0641077430921477 1105 4 1.7293658584481868 1105 5 1.2141691904777545 1105 6 1.0619409887647855 1105 7 1.069882464919516 1105 8 1.385015894764912 1105 9 1.6574993837292915 1105 10-11 1.4441278737875045 1105 12-13 1.468781669991575 1105 14-15 1.6873702104652466 1105 16-17 1.4358135027058943 1105 18-19 1.2241893124491092 1105 20-21 1.4060348977434671 1105 22-23 1.3294112857770628 1105 24-25 1.3351243877228534 1105 26-27 1.3662082738794368 1105 28-29 0.8487685292761888 1105 30-31 1.2722504226233795 1105 32-33 1.2483247311243169 1105 34-35 1.085258608544084 1105 36-37 1.3122835904012362 1105 38-39 1.20394194094537 1105 40-41 1.371182343109684 1105 42-43 1.6671730711128028 1105 44-45 1.843181264541819 1105 46-47 1.941572220605341 1105 48-49 1.6445731376515305 1105 50-51 1.6913241607983025 1105 52-53 1.7400819564358976 1105 54-55 1.7918215262775448 1105 56-57 2.055975716173542 1105 58-59 1.7778773156737948 1105 60-61 2.0186467097600875 1105 62-63 1.9044336112962341 1105 64-65 1.5935185715682145 1105 66-67 1.7854787562607193 1105 68-69 1.1917878487724707 1105 70-71 1.2223027210706974 1105 72-73 1.342848573799234 1105 74-75 1.6480710156516132 1105 76-77 1.529593397107707 1105 78-79 1.3465569664650054 1105 80-81 1.4101446339866435 1105 82-83 1.5180878820158092 1105 84-85 1.456828012776075 1105 86-87 1.4244674813575173 1105 88-89 1.5109869628679498 1105 90-91 1.5551266519971279 1105 92-93 1.5276390981223607 1105 94-95 1.3840046667086092 1105 96-97 1.4355911570787718 1105 98-99 1.3544098034261083 1105 100-101 1.471670200322766 1105 102-103 1.4121888060213799 1105 104-105 1.580474531906411 1105 106-107 1.6304199120563005 1105 108-109 1.682497685950878 1105 110-111 1.614182247204873 1105 112-113 1.662966756480273 1105 114-115 1.5690560133750537 1105 116-117 1.739096094831094 1105 118-119 1.7248897504684457 1105 120-121 1.7344382189024685 1105 122-123 1.84236365449064 1105 124-125 1.4983858993324084 1106 1 -0.31235763275433115 1106 2 -0.05051477117187275 1106 3 -0.12290265829495439 1106 4 -0.4430842855839785 1106 5 -1.1201921982955376 1106 6 -0.3350960727669303 1106 7 -0.37193240898292856 1106 8 0.02894367715623858 1106 9 0.15590239552077279 1106 10-11 -2.339633334173481 1106 12-13 -0.5248835976362187 1106 14-15 -0.11413917923394479 1106 16-17 -0.36042104043106704 1106 18-19 -3.210476892931961 1106 20-21 -5.312318175410649 1106 22-23 -6.139208014470572 1106 24-25 -6.153669117509036 1106 26-27 -5.088937701798876 1106 28-29 -1.778033900521777 1106 30-31 -3.4725026200833895 1106 32-33 -4.2553505067611646 1106 34-35 -3.3619027380521445 1106 36-37 -5.794540077667797 1106 38-39 -6.119804835722729 1106 40-41 -2.3618355565600737 1106 42-43 -1.0234158604037589 1106 44-45 -0.2415242478626638 1106 46-47 -0.06579644246296468 1106 48-49 0.09300896768796107 1106 50-51 -4.3143370053400076E-5 1106 52-53 -0.2544425128101011 1106 54-55 -0.06340387454490681 1106 56-57 0.05851457553363559 1106 58-59 0.2657857577617584 1106 60-61 0.380074446917142 1106 62-63 0.08156874697097294 1106 64-65 0.08735120600603707 1106 66-67 -0.1842057643215007 1106 68-69 -0.08389957179458563 1106 70-71 0.15496598404030237 1106 72-73 0.19403377414338507 1106 74-75 0.062168417207793425 1106 76-77 -0.08480164596468143 1106 78-79 -0.0029058687443530573 1106 80-81 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0.7883279145812843 2208 32-33 0.8084879080027818 2208 34-35 0.8308847108893715 2208 36-37 1.1066159227529404 2208 38-39 0.8692644535531713 2208 40-41 0.5918891145493994 2208 42-43 0.4929474919379828 2208 44-45 0.539095378630897 2208 46-47 0.6024289010783086 2208 48-49 0.9733158883510242 2208 50-51 0.9358662799414184 2208 52-53 0.7979390790876586 2208 54-55 0.527549225528471 2208 56-57 0.9348675471312191 2208 58-59 0.7421171602015484 2208 60-61 1.1013296779857455 2208 62-63 1.1978863626805847 2208 64-65 0.9705275446712385 2208 66-67 0.7919037481466624 2208 68-69 0.8480995416472865 2208 70-71 1.128340533561083 2208 72-73 1.0768880780825612 2208 74-75 0.9949987415027444 2208 76-77 1.051451036875413 2208 78-79 0.9721366747758857 2208 80-81 0.9324949482760161 2208 82-83 0.8366761530123519 2208 84-85 0.6415850526495852 2208 86-87 0.8094412558373989 2208 88-89 0.7577968612214185 2208 90-91 0.7803999368545611 2208 92-93 0.8964502078769918 2208 94-95 0.9423529130304864 2208 96-97 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-4.989230707650872 2216 70-71 -6.997253932906759 2216 72-73 -7.341559623454305 2216 74-75 -5.226592444702149 2216 76-77 -4.518634101138126 2216 78-79 -4.909094668201558 2216 80-81 -4.964722121352711 2216 82-83 -5.165514686819741 2216 84-85 -4.864571508400498 2216 86-87 -5.183287633267241 2216 88-89 -5.328978520419959 2216 90-91 -5.384546973389803 2216 92-93 -5.162694462362342 2216 94-95 -8.133733211135887 2216 96-97 -9.893897182288777 2216 98-99 -6.988450468730832 2216 100-101 -6.114126983446926 2216 102-103 -7.088134518457174 2216 104-105 -9.782997115071975 2216 106-107 -6.610078964356358 2216 108-109 -8.859320299827047 2216 110-111 -6.3844683862317595 2216 112-113 -5.786351585014195 2216 114-115 -6.207939891427593 2216 116-117 -5.773205026121726 2216 118-119 -6.0250004401467905 2216 120-121 -6.8530734447960135 2216 122-123 -9.478092834591049 2216 124-125 -8.131023694101467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 269888.0 3 38786.0 4 22492.0 5 18189.0 6 18108.0 7 20510.0 8 21865.0 9 23385.0 10 24916.0 11 25102.0 12 25731.0 13 25724.0 14 26379.0 15 22117.0 16 18324.0 17 16318.0 18 15052.0 19 14197.0 20 14546.0 21 15821.0 22 19815.0 23 26829.0 24 35720.0 25 44868.0 26 53813.0 27 62073.0 28 69931.0 29 79485.0 30 88240.0 31 99741.0 32 117201.0 33 141307.0 34 173997.0 35 215874.0 36 275624.0 37 278009.0 38 77576.0 39 179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.790689821666945 18.945993581146116 13.84152152714443 23.42179507004251 2 14.956858966980397 20.21380513056627 40.70047414139726 24.128861761056072 3 18.671064360426907 25.790061112225953 29.119240396439004 26.419634130908136 4 11.10880238208265 16.634125415768363 38.41159530459714 33.84547689755183 5 13.342378479019718 38.41789598240179 34.104536491484986 14.135189047093508 6 31.037226307862426 37.16900994398675 17.864419096048493 13.92934465210233 7 28.104139114864026 32.78785361540074 21.223465545206498 17.884541724528734 8 25.76156719520466 35.65276878535042 20.10530302104839 18.480360998396534 9 26.03374799114409 16.84026176801389 20.618408410751627 36.507581830090395 10-11 24.265517720003597 27.339467918820315 27.54786664374346 20.847147717432623 12-13 25.256452191321415 24.619843862684284 28.123337444975586 22.000366501018714 14-15 22.41748518876504 25.92802128994944 25.847326785156543 25.807166736128966 16-17 22.231202328494916 29.492692315347963 26.008641003795884 22.267464352361237 18-19 22.077846063798653 27.93658958130717 28.347074293811197 21.63849006108298 20-21 22.92199139047159 27.175614570298983 28.356179086714423 21.546214952515005 22-23 22.59063840923665 27.121465144409363 27.999303956295073 22.28859249005891 24-25 22.253159193808226 27.29586804546283 28.185063479473243 22.2659092812557 26-27 22.181753407511856 27.65994005080627 27.81322192630332 22.345084615378553 28-29 22.076636976892104 27.60910617779028 28.035497684008426 22.278759161309186 30-31 22.19210481776234 27.24969425591238 28.254654925410662 22.303546000914622 32-33 22.038816701057254 27.488831597515052 27.84603131566461 22.626320385763083 34-35 22.49101821895977 27.329011147833288 27.979684059656645 22.200286573550297 36-37 22.538957140495544 27.080677884883464 27.915532159258145 22.464832815362847 38-39 22.209160037597023 27.294768141844028 28.057861580690734 22.438210239868216 40-41 22.45868359070886 27.2383983916904 27.96215742118593 22.34076059641481 42-43 22.36332027736767 27.22892055356874 27.7386392210247 22.669119948038883 44-45 22.399321131077368 27.316722058841226 27.808752114808065 22.475204695273344 46-47 22.516324022612753 27.565160627515546 27.325957713101918 22.592557636769783 48-49 22.552564612373054 27.499575352890616 27.28585272883553 22.662007305900804 50-51 22.51583788516324 27.660615930127086 27.183247715775856 22.64029846893382 52-53 22.811436498818633 27.45975568662149 27.144225293272612 22.584582521287267 54-55 22.519428062128025 27.451634534687148 27.261634915001675 22.767302488183155 56-57 22.414909118495217 27.679267943050416 27.219190485151273 22.686632453303094 58-59 22.5816934087585 27.292639582605023 27.21456326329849 22.911103745337986 60-61 22.636231743806135 27.299327964327336 27.471411826779292 22.593028465087237 62-63 22.504189208787327 27.493800916818 27.31726694915254 22.68474292524213 64-65 22.37151907152252 27.255365789979262 27.325494214257212 23.047620924241006 66-67 22.297368528393303 27.633417480468847 27.09452374961107 22.97469024152678 68-69 22.48140116632495 27.338546556937406 27.345384109881643 22.834668166855995 70-71 22.219741730208202 27.206739179351658 27.638449876831423 22.93506921360872 72-73 22.428862102983633 27.394471635573154 27.284943085150715 22.891723176292498 74-75 22.394895794255522 27.467143113846078 27.22174102878544 22.916220063112963 76-77 22.514472673303107 27.286065622063372 27.33583991358269 22.863621791050832 78-79 22.44399008735152 27.45835160341523 27.2573790602691 22.840279248964148 80-81 22.316022058516385 27.671111524447767 27.193142849936986 22.81972356709886 82-83 22.50869977543437 27.637454087392026 27.045134401456828 22.808711735716773 84-85 22.458160615789353 27.558075063218663 27.047675756151136 22.936088564840848 86-87 22.475662453945013 27.306778888564214 27.240081786213423 22.97747687127735 88-89 22.451451480248576 27.33668753618208 27.191269561968745 23.020591421600596 90-91 22.349388177493847 27.5983860756563 27.271159900242026 22.781065846607827 92-93 22.4376391811038 27.409618731949063 27.215696180383837 22.9370459065633 94-95 22.312688885447294 27.22525726252107 27.253788348991936 23.208265503039705 96-97 22.463477564402535 27.20508422646521 27.231131922120937 23.10030628701132 98-99 22.15991084538468 27.164750036845636 27.213536022421064 23.461803095348625 100-101 22.136757374167306 27.198319846644036 27.364597337156333 23.300325442032324 102-103 22.314473280042307 26.978676167656506 27.45483558836041 23.25201496394078 104-105 22.190245621930316 26.996836996247865 27.464637625932493 23.34827975588933 106-107 22.091795951813932 27.11911493241234 27.457117249072933 23.331971866700794 108-109 22.09478368944404 27.032219014585685 27.2623097977928 23.61068749817748 110-111 21.87657625280573 27.102346882802454 27.5784540427732 23.442622821618624 112-113 22.069272484632727 27.173786898059653 27.49438301650387 23.262557600803746 114-115 21.885783798545198 27.327783340643215 27.356136221504762 23.430296639306828 116-117 21.695583510748868 27.227933563012975 27.563428722302735 23.513054203935425 118-119 21.81124836510903 27.243046762906033 27.273665871830854 23.67203900015408 120-121 21.685174698242044 27.279061235121947 27.49694882498728 23.538815241648724 122-123 21.79891456836517 27.204911081617016 27.496603100242435 23.49957124977538 124-125 21.781136524032757 27.28349623274574 27.19961519202943 23.735752051192076 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 778.0 1 822.5 2 1049.0 3 1126.5 4 901.0 5 712.5 6 628.5 7 699.5 8 899.0 9 1032.5 10 1017.5 11 1062.0 12 1189.0 13 1287.0 14 1439.5 15 1608.0 16 1809.5 17 2031.0 18 2270.0 19 2594.0 20 3065.5 21 3760.5 22 4669.5 23 5945.5 24 7694.5 25 10094.5 26 13441.0 27 17578.0 28 21853.5 29 26518.5 30 31718.5 31 37459.0 32 43669.5 33 49194.0 34 54380.5 35 59098.5 36 64512.0 37 70093.0 38 73207.0 39 75261.5 40 77006.0 41 79450.5 42 82289.0 43 85100.0 44 88269.0 45 90440.5 46 90631.5 47 90693.0 48 92480.5 49 92491.0 50 90485.0 51 88184.5 52 85993.5 53 84616.5 54 81967.5 55 76565.5 56 70127.0 57 64405.0 58 59882.5 59 54107.0 60 46002.0 61 37654.5 62 30200.5 63 23302.0 64 17554.0 65 13754.5 66 10701.5 67 8287.0 68 6418.5 69 4814.5 70 3560.5 71 2789.0 72 2315.0 73 1921.0 74 1500.5 75 1149.0 76 834.5 77 553.0 78 399.5 79 273.0 80 184.5 81 142.0 82 110.5 83 78.0 84 61.0 85 48.0 86 37.0 87 26.5 88 19.0 89 17.0 90 11.5 91 8.5 92 9.5 93 5.5 94 2.5 95 5.0 96 5.0 97 3.0 98 1.0 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10639421341575864 2 0.036883327317462994 3 0.0492959855493015 4 0.057689306829878016 5 0.06166923851691195 6 0.004649821178910933 7 0.0034676632520691704 8 0.004058742215490052 9 0.008590347601716808 10-11 0.06809229658608554 12-13 0.008747968658629044 14-15 0.015151324095688591 16-17 0.025317882266527748 18-19 0.024017508547001812 20-21 0.14040095644457334 22-23 0.021081816362011434 24-25 0.019485903160775055 26-27 0.009851316057014689 28-29 0.006226031748033284 30-31 0.0027780711280781423 32-33 0.008511537073260692 34-35 0.02019519791688011 36-37 0.004078444847604081 38-39 0.01170336347573345 40-41 0.03574057465484929 42-43 0.017732368902626437 44-45 0.115358911027642 46-47 0.0054182238313580785 48-49 0.013043142459487447 50-51 0.015072513567232474 52-53 0.0164319951831005 54-55 0.014126787225759063 56-57 0.00884648181919919 58-59 0.018559879451415674 60-61 0.034972172002402144 62-63 0.010245368699295276 64-65 0.009634587103760365 66-67 0.0122747398070403 68-69 0.010816745030602128 70-71 0.006029005426892989 72-73 0.0014776974085522034 74-75 0.0023643158536835254 76-77 0.010934960823286304 78-79 0.02244129797787946 80-81 0.004649821178910933 82-83 0.006560976493971782 84-85 0.004373984329314522 86-87 0.005536439624042255 88-89 0.008629752865944867 90-91 0.007506702835445193 92-93 0.00476803697159511 94-95 0.005871384369980755 96-97 0.0031327185061306713 98-99 0.004649821178910933 100-101 0.004196660640288258 102-103 0.0012412658231838508 104-105 0.004433092225656609 106-107 0.0010836447662716157 108-109 0.0014974000406662326 110-111 0.0026795579675079955 112-113 0.004551308018340786 114-115 0.0027977737601921717 116-117 0.005497034359814196 118-119 0.003979931687033934 120-121 0.007289973882190871 122-123 0.0048862527642792855 124-125 0.05524618044773838 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2537732.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.91472154167595 #Duplication Level Percentage of deduplicated Percentage of total 1 83.06583528881058 54.75261402688669 2 9.715691443221273 12.80814192129548 3 3.1566785566820506 6.242147641808308 4 1.3949395580941915 3.6778821015698857 5 0.728044817139025 2.399443569578961 6 0.44622880463068887 1.7647828440664064 7 0.2788328129575448 1.2865431055945145 8 0.19720427456800202 1.039893187598245 9 0.1499736358955306 0.8896923403781934 >10 0.7858900160854054 9.385320733715602 >50 0.05544533160577235 2.485798489474412 >100 0.02448966326307454 2.795936490661777 >500 4.971980312922052E-4 0.19733243793493593 >1k 2.4859901564442023E-4 0.2744711094365898 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2678 0.10552729760274135 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.970263211402938E-5 0.0 12-13 0.0 0.0 0.0 3.940526422805876E-5 0.0 14-15 0.0 0.0 0.0 3.940526422805876E-5 0.0 16-17 0.0 0.0 0.0 7.881052845611752E-5 0.0 18-19 0.0 0.0 0.0 7.881052845611752E-5 3.940526422805876E-5 20-21 0.0 0.0 0.0 1.3791842479820565E-4 3.940526422805876E-5 22-23 0.0 0.0 0.0 2.561342174823819E-4 3.940526422805876E-5 24-25 0.0 0.0 0.0 4.137552743946169E-4 3.940526422805876E-5 26-27 0.0 0.0 0.0 8.86618445131322E-4 3.940526422805876E-5 28-29 0.0 0.0 0.0 0.001517102672780262 3.940526422805876E-5 30-31 0.0 0.0 0.0 0.0036646895732094643 3.940526422805876E-5 32-33 0.0 0.0 0.0 0.008472131809032632 3.940526422805876E-5 34-35 0.0 0.0 0.0 0.013693329319250417 3.940526422805876E-5 36-37 3.940526422805876E-5 0.0 0.0 0.024273642764484193 3.940526422805876E-5 38-39 3.940526422805876E-5 1.970263211402938E-5 0.0 0.04421270646388192 3.940526422805876E-5 40-41 3.940526422805876E-5 3.940526422805876E-5 0.0 0.06895921239910283 3.940526422805876E-5 42-43 3.940526422805876E-5 3.940526422805876E-5 0.0 0.0938239341270079 3.940526422805876E-5 44-45 3.940526422805876E-5 3.940526422805876E-5 0.0 0.12158494277567528 3.940526422805876E-5 46-47 3.940526422805876E-5 3.940526422805876E-5 0.0 0.15844856746102426 3.940526422805876E-5 48-49 3.940526422805876E-5 3.940526422805876E-5 0.0 0.19730215798989018 3.940526422805876E-5 50-51 3.940526422805876E-5 3.940526422805876E-5 0.0 0.24474609612047293 3.940526422805876E-5 52-53 3.940526422805876E-5 3.940526422805876E-5 0.0 0.29069263421038943 3.940526422805876E-5 54-55 3.940526422805876E-5 3.940526422805876E-5 0.0 0.3392990276356999 3.940526422805876E-5 56-57 3.940526422805876E-5 3.940526422805876E-5 0.0 0.3994708661119456 3.940526422805876E-5 58-59 5.910789634208813E-5 3.940526422805876E-5 0.0 0.4672282179520927 3.940526422805876E-5 60-61 7.881052845611752E-5 3.940526422805876E-5 0.0 0.5479301990911569 3.940526422805876E-5 62-63 7.881052845611752E-5 3.940526422805876E-5 0.0 0.629774932892835 3.940526422805876E-5 64-65 7.881052845611752E-5 3.940526422805876E-5 0.0 0.7088612981985489 3.940526422805876E-5 66-67 7.881052845611752E-5 3.940526422805876E-5 0.0 0.8011681296527766 3.940526422805876E-5 68-69 7.881052845611752E-5 3.940526422805876E-5 0.0 0.9094537957514821 3.940526422805876E-5 70-71 7.881052845611752E-5 3.940526422805876E-5 0.0 1.0364372597264013 3.940526422805876E-5 72-73 7.881052845611752E-5 3.940526422805876E-5 0.0 1.1723854213132041 3.940526422805876E-5 74-75 7.881052845611752E-5 3.940526422805876E-5 0.0 1.306501238113402 7.881052845611752E-5 76-77 7.881052845611752E-5 3.940526422805876E-5 0.0 1.4556895684808326 7.881052845611752E-5 78-79 7.881052845611752E-5 3.940526422805876E-5 0.0 1.6285998679135543 7.881052845611752E-5 80-81 7.881052845611752E-5 3.940526422805876E-5 0.0 1.8311823313100044 7.881052845611752E-5 82-83 7.881052845611752E-5 3.940526422805876E-5 0.0 2.04826593194238 7.881052845611752E-5 84-85 7.881052845611752E-5 3.940526422805876E-5 0.0 2.260226848225108 7.881052845611752E-5 86-87 1.1821579268417627E-4 3.940526422805876E-5 0.0 2.479162496276203 7.881052845611752E-5 88-89 1.1821579268417627E-4 3.940526422805876E-5 0.0 2.72280524499829 7.881052845611752E-5 90-91 1.1821579268417627E-4 3.940526422805876E-5 0.0 3.010286350174092 7.881052845611752E-5 92-93 1.1821579268417627E-4 3.940526422805876E-5 0.0 3.3194600533074414 7.881052845611752E-5 94-95 1.1821579268417627E-4 3.940526422805876E-5 0.0 3.6271757616643523 7.881052845611752E-5 96-97 1.1821579268417627E-4 3.940526422805876E-5 0.0 3.9359751147875346 7.881052845611752E-5 98-99 1.1821579268417627E-4 3.940526422805876E-5 0.0 4.268476734343895 7.881052845611752E-5 100-101 1.1821579268417627E-4 3.940526422805876E-5 0.0 4.655436429063432 7.881052845611752E-5 102-103 1.1821579268417627E-4 3.940526422805876E-5 0.0 5.0804419063951585 7.881052845611752E-5 104-105 1.1821579268417627E-4 3.940526422805876E-5 0.0 5.501999423106932 7.881052845611752E-5 106-107 1.1821579268417627E-4 3.940526422805876E-5 0.0 5.915104510641786 7.881052845611752E-5 108-109 1.1821579268417627E-4 3.940526422805876E-5 0.0 6.349468738227677 7.881052845611752E-5 110-111 1.1821579268417627E-4 3.940526422805876E-5 0.0 6.836773938303966 7.881052845611752E-5 112-113 1.1821579268417627E-4 3.940526422805876E-5 0.0 7.373000773919389 7.881052845611752E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6860 0.0 55.88593 1 GGTATCA 3640 0.0 50.53565 1 ATCAACG 7965 0.0 45.946262 3 CAACGCA 8305 0.0 44.40703 5 TCAACGC 8210 0.0 44.342068 4 TATCAAC 8870 0.0 42.865948 2 AACGCAG 8405 0.0 42.576675 6 CGCAGAG 9745 0.0 35.629307 8 ACGCAGA 9965 0.0 35.43546 7 GTACATG 11090 0.0 34.730694 1 TACATGG 11020 0.0 33.63889 2 GCAGAGT 10485 0.0 31.24028 9 ACATGGG 11340 0.0 30.487648 3 CGCATAG 325 0.0 29.31955 8 GAGTACT 4930 0.0 24.97888 12-13 AGAGTAC 10210 0.0 24.1063 10-11 CAGAGTA 10650 0.0 24.004591 10-11 CATGGGA 7090 0.0 23.026194 4 AACGCAT 460 0.0 22.005238 6 AGTACTT 5555 0.0 21.900742 12-13 >>END_MODULE