FastQCFastQC Report
Thu 26 May 2016
SRR1364510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537732
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58750.23150592733984518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52090.20526202136395807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31680.12483587707449012No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49700.068.919731
GTATCAA88600.050.0429841
ATCAACG107600.038.939513
TATCAAC113150.038.662412
TCAACGC109650.038.1027954
CAACGCA109150.038.1026735
AACGCAG112350.036.8585436
GTACATG105150.033.256491
CGCAGAG134550.031.248118
TACATGG106950.031.040012
ACGCAGA132650.030.9488017
GCAGAGT144350.027.9319299
ACATGGG107450.027.9003093
CTACGCG1107.678006E-427.0909844
GAGTACT86500.026.84841512-13
TGGTATC22800.026.1404232
CAGAGTA145450.025.33035510-11
GTACTTT95900.024.65086714-15
CATACCG1751.7523014E-523.799185
AGAGTAC141500.023.1794310-11
Produced by FastQC (version 0.11.5)