##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364510_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2537732 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.060972947498 31.0 28.0 31.0 25.0 34.0 2 29.233179074858967 31.0 28.0 33.0 25.0 34.0 3 29.31127636803256 31.0 28.0 34.0 25.0 34.0 4 32.748025402209535 35.0 32.0 37.0 26.0 37.0 5 31.928318277895382 35.0 32.0 37.0 22.0 37.0 6 31.99135212071251 35.0 32.0 37.0 23.0 37.0 7 31.451720670267783 35.0 32.0 37.0 17.0 37.0 8 31.57041523691233 35.0 32.0 37.0 18.0 37.0 9 32.14856139261356 37.0 31.0 39.0 13.0 39.0 10-11 32.353283561857594 37.0 31.5 39.0 17.0 39.0 12-13 32.10836073312706 37.0 30.5 39.0 14.0 39.0 14-15 33.08217692017912 38.0 31.5 40.0 16.5 41.0 16-17 33.16014969271775 38.0 32.0 40.0 17.0 41.0 18-19 33.041055162641285 38.0 31.0 40.0 15.5 41.0 20-21 32.93812467195117 38.0 31.0 40.0 13.5 41.0 22-23 32.895325432315154 38.0 31.0 40.0 13.0 41.0 24-25 32.746755961622426 38.0 31.0 40.0 10.5 41.0 26-27 32.797622641003855 38.0 31.0 40.0 10.5 41.0 28-29 32.66911911896134 37.5 31.0 40.0 10.0 41.0 30-31 32.153476214194406 37.0 30.0 40.0 9.0 41.0 32-33 32.08993956020572 37.0 30.0 40.0 8.5 41.0 34-35 31.94196530602916 37.0 30.0 40.0 8.0 41.0 36-37 31.897319732737735 37.0 30.0 40.0 8.0 41.0 38-39 31.685048894051853 37.0 30.0 40.0 2.0 41.0 40-41 31.369929330599135 36.5 30.0 40.0 2.0 41.0 42-43 31.256886266950175 36.5 29.5 40.0 2.0 41.0 44-45 31.17210978148993 36.0 29.5 40.0 2.0 41.0 46-47 31.002642911071774 36.0 29.0 40.0 2.0 41.0 48-49 30.721085599267376 36.0 28.5 40.0 2.0 41.0 50-51 30.408885571841314 36.0 28.0 40.0 2.0 41.0 52-53 30.30208962175675 36.0 28.0 40.0 2.0 41.0 54-55 29.82895376659159 35.0 27.0 40.0 2.0 41.0 56-57 29.84822530511496 35.0 27.0 39.5 2.0 41.0 58-59 29.63782759566416 35.0 27.0 39.0 2.0 41.0 60-61 29.37083742491327 35.0 26.0 39.0 2.0 41.0 62-63 29.108304777651856 34.5 26.0 39.0 2.0 41.0 64-65 28.61708486160083 34.0 26.0 38.5 2.0 40.0 66-67 28.264270616440193 34.0 24.5 38.0 2.0 40.0 68-69 28.0807740927726 34.0 24.5 37.5 2.0 40.0 70-71 28.149437962716313 34.0 26.0 37.0 2.0 40.0 72-73 27.947607351761338 34.0 25.5 37.0 2.0 39.0 74-75 27.572126213485113 34.0 24.5 36.0 2.0 39.0 76-77 27.46941678632732 34.0 25.5 36.0 2.0 39.0 78-79 27.071966228112345 33.0 24.0 35.5 2.0 38.0 80-81 26.85126423909223 33.0 24.0 35.0 2.0 37.0 82-83 26.69189220926402 33.0 24.0 35.0 2.0 37.0 84-85 26.278395039350098 33.0 22.5 35.0 2.0 36.0 86-87 26.159068609293655 33.0 22.5 35.0 2.0 36.0 88-89 26.085394951082307 33.0 23.0 35.0 2.0 36.0 90-91 25.916872427821378 33.0 22.0 35.0 2.0 35.0 92-93 25.94693923550635 33.0 23.0 35.0 2.0 35.0 94-95 25.934151045106418 33.0 23.0 35.0 2.0 35.0 96-97 25.807784864595632 33.0 23.0 35.0 2.0 35.0 98-99 25.6871891909784 33.0 21.0 35.0 2.0 35.0 100-101 25.607970818037522 33.0 20.0 35.0 2.0 35.0 102-103 24.443444973700927 30.5 18.5 34.0 2.0 35.0 104-105 25.384231077198066 32.5 20.0 35.0 2.0 35.0 106-107 25.514528917947207 33.0 20.0 35.0 2.0 35.0 108-109 25.57734918423222 33.0 20.0 35.0 2.0 35.0 110-111 25.512952313325442 33.0 20.0 35.0 2.0 35.0 112-113 25.447375451781355 33.0 19.5 35.0 2.0 35.0 114-115 25.385215814751124 33.0 19.0 35.0 2.0 35.0 116-117 25.300480507791995 33.0 19.0 35.0 2.0 35.0 118-119 25.175264763970347 33.0 17.5 35.0 2.0 35.0 120-121 25.013201354595363 33.0 11.0 35.0 2.0 35.0 122-123 24.720031508449274 33.0 2.0 35.0 2.0 35.0 124-125 23.937101317239172 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6489565034078311 1101 2 0.6368094100319084 1101 3 0.639860533605205 1101 4 0.6435622396946457 1101 5 0.812222935937001 1101 6 0.9126959458082382 1101 7 1.30856306950648 1101 8 1.24666673349126 1101 9 1.8820717551151844 1101 10-11 1.5518997102368175 1101 12-13 1.658890402860962 1101 14-15 1.565390300567067 1101 16-17 1.5174109130866427 1101 18-19 1.4692271420042147 1101 20-21 1.8109147216554078 1101 22-23 1.894979899729897 1101 24-25 2.0160979666927332 1101 26-27 1.6580652147669994 1101 28-29 1.7241722353069733 1101 30-31 2.134429619668502 1101 32-33 1.9536489745862866 1101 34-35 2.085845607709267 1101 36-37 1.9073339383146752 1101 38-39 2.0469148314903407 1101 40-41 2.2312612552445046 1101 42-43 2.1247512875676584 1101 44-45 1.9243106695225372 1101 46-47 1.7323452494829574 1101 48-49 1.8049590011530228 1101 50-51 2.0117471597716516 1101 52-53 1.8787030597076644 1101 54-55 2.2178896255794527 1101 56-57 1.936247573757317 1101 58-59 2.096414959668291 1101 60-61 2.211076440835395 1101 62-63 2.157573431001211 1101 64-65 2.2440265763471317 1101 66-67 2.4756183390111133 1101 68-69 2.470950460385044 1101 70-71 2.0903332506249903 1101 72-73 2.310613012178628 1101 74-75 2.361121248822421 1101 76-77 2.103209509727595 1101 78-79 2.272964571231711 1101 80-81 2.3088324097231165 1101 82-83 2.15012281302311 1101 84-85 2.4737751968826878 1101 86-87 2.240821894902542 1101 88-89 2.0670152978905136 1101 90-91 2.0543222229578326 1101 92-93 2.039595830552855 1101 94-95 1.9926146189731284 1101 96-97 1.9620317297868262 1101 98-99 1.9689193642547735 1101 100-101 2.047581142018835 1101 102-103 1.8169929612889781 1101 104-105 1.8993015673902853 1101 106-107 1.9368630542501393 1101 108-109 1.960646473811753 1101 110-111 1.94176062781969 1101 112-113 1.7990623401272856 1101 114-115 1.7635365500385234 1101 116-117 1.8734311884496577 1101 118-119 1.7770038820170981 1101 120-121 1.7480833474351094 1101 122-123 1.8296004540423425 1101 124-125 1.680341026767195 1102 1 0.41692178754275133 1102 2 -1.8008602555050182 1102 3 -0.06225915025049389 1102 4 0.3432765637522479 1102 5 0.8468783337503822 1102 6 0.3833475711785681 1102 7 1.2122466869083972 1102 8 0.8571637106382042 1102 9 1.698729581314403 1102 10-11 1.4715721039445526 1102 12-13 1.5277609483114247 1102 14-15 1.4436578610777033 1102 16-17 1.5151545846896823 1102 18-19 1.558180072191817 1102 20-21 1.746203616140292 1102 22-23 1.6665336737547918 1102 24-25 1.8179088784064348 1102 26-27 1.5610716689460773 1102 28-29 1.51810039687755 1102 30-31 1.9046674333456437 1102 32-33 1.5590604824000849 1102 34-35 1.8290885820252214 1102 36-37 1.226493183070854 1102 38-39 1.0216862358821501 1102 40-41 1.251408463697878 1102 42-43 1.0424359155638534 1102 44-45 1.037412905637776 1102 46-47 0.8965615886058416 1102 48-49 0.9522465786251182 1102 50-51 1.078353320901357 1102 52-53 1.006179506814366 1102 54-55 1.2067546549864439 1102 56-57 0.8381295472660213 1102 58-59 0.7530191524226311 1102 60-61 0.8185668936408206 1102 62-63 1.0241031342064346 1102 64-65 1.18520968985516 1102 66-67 1.4188953229752954 1102 68-69 1.3346330036637895 1102 70-71 0.6246656460778546 1102 72-73 1.0003339085433467 1102 74-75 1.2527471319761894 1102 76-77 1.1669185361382652 1102 78-79 1.0051024896677454 1102 80-81 1.0784596188194335 1102 82-83 0.7349719739823826 1102 84-85 0.8354546343891265 1102 86-87 0.8075544759924647 1102 88-89 0.931657089293239 1102 90-91 0.6190591610077156 1102 92-93 0.6434717695814385 1102 94-95 0.6435553952401598 1102 96-97 0.7908385651903345 1102 98-99 0.5607633058939108 1102 100-101 0.3961261069580253 1102 102-103 0.2683658935380855 1102 104-105 0.6110865100713987 1102 106-107 0.8262769389122724 1102 108-109 0.7081799770147335 1102 110-111 0.6433792561771519 1102 112-113 0.6287661066511774 1102 114-115 0.989417318284076 1102 116-117 0.598142731523275 1102 118-119 0.01772920473880646 1102 120-121 0.3821381292477817 1102 122-123 0.7251459300192344 1102 124-125 0.808359988995818 1103 1 0.7474115846723421 1103 2 0.6532608143975942 1103 3 0.5899986599787042 1103 4 0.6390185323555997 1103 5 1.005586269451527 1103 6 0.9839911887475807 1103 7 1.2571257980993131 1103 8 1.0336749919535606 1103 9 1.8359294898038314 1103 10-11 1.5399029542167213 1103 12-13 1.5486023661773913 1103 14-15 1.1974128570018294 1103 16-17 1.3000431474918557 1103 18-19 1.4367904976500228 1103 20-21 1.6485747366425585 1103 22-23 1.5061124195121565 1103 24-25 1.838029726634339 1103 26-27 1.39960218371332 1103 28-29 1.6148876289719922 1103 30-31 2.0546946417609604 1103 32-33 1.885253366286669 1103 34-35 1.8174237427799724 1103 36-37 1.8182341767112824 1103 38-39 1.8413795359794989 1103 40-41 2.1739679303495265 1103 42-43 2.149226980043803 1103 44-45 2.0243877407386712 1103 46-47 1.9547277401451133 1103 48-49 1.932356313949569 1103 50-51 1.9653064133650773 1103 52-53 1.8422012484370107 1103 54-55 2.0681190920214405 1103 56-57 2.012381122450016 1103 58-59 2.110044383012525 1103 60-61 2.016256131308076 1103 62-63 2.136201456580313 1103 64-65 2.066329617651089 1103 66-67 2.359614996022845 1103 68-69 2.49922365746216 1103 70-71 2.2137913409672088 1103 72-73 2.290251748133027 1103 74-75 2.3116146404995064 1103 76-77 2.1361734706833566 1103 78-79 2.2790904710430127 1103 80-81 2.273996753899482 1103 82-83 2.1857551464366836 1103 84-85 2.279399211652404 1103 86-87 2.194352028389112 1103 88-89 2.1452188008452318 1103 90-91 2.224854179363092 1103 92-93 1.9732514911133592 1103 94-95 1.8781391109243302 1103 96-97 1.7700239372462327 1103 98-99 1.9758895044972071 1103 100-101 2.017389657902882 1103 102-103 1.793965994128694 1103 104-105 1.9065267512352264 1103 106-107 1.8504979133476454 1103 108-109 1.9023574839300217 1103 110-111 1.85536102812409 1103 112-113 1.7751328923221656 1103 114-115 1.7182761386296548 1103 116-117 1.8154787784696147 1103 118-119 1.844716623811049 1103 120-121 1.8041468331507247 1103 122-123 1.9234928998983563 1103 124-125 1.6860558477367782 1104 1 0.6916367235782843 1104 2 0.5882206007433695 1104 3 0.6173812614337635 1104 4 0.625739009086935 1104 5 1.0130898764014304 1104 6 1.1076735968087306 1104 7 1.311271775966457 1104 8 1.17376518488701 1104 9 1.6704549072890984 1104 10-11 1.229462800806239 1104 12-13 1.3446360642987116 1104 14-15 1.4873121022283797 1104 16-17 1.2111992229001274 1104 18-19 1.118479087409355 1104 20-21 1.5104760405165791 1104 22-23 1.4856935816407173 1104 24-25 1.577331426889124 1104 26-27 1.5112334459922963 1104 28-29 1.4170399516174967 1104 30-31 1.9162016753132676 1104 32-33 1.83426083152181 1104 34-35 1.8020253762678138 1104 36-37 1.7643582404391758 1104 38-39 1.854073169712919 1104 40-41 1.9796506991056688 1104 42-43 2.0261990511237364 1104 44-45 1.7294932056514902 1104 46-47 1.8048752797448167 1104 48-49 1.6362636191742723 1104 50-51 1.8968268866429483 1104 52-53 1.615941645892196 1104 54-55 2.009947431903562 1104 56-57 1.9410160358064772 1104 58-59 1.921976000614439 1104 60-61 2.095447387908024 1104 62-63 2.17623716154716 1104 64-65 2.2557052795647685 1104 66-67 2.1810062234602228 1104 68-69 2.4086352545735323 1104 70-71 2.2189813677507324 1104 72-73 2.337878116087964 1104 74-75 2.3681569377428744 1104 76-77 2.1343273070966653 1104 78-79 2.2352178251533985 1104 80-81 2.140082023730031 1104 82-83 2.1361310462555707 1104 84-85 2.4477747028115395 1104 86-87 2.3339736056238856 1104 88-89 2.1410849580622013 1104 90-91 2.272538105029227 1104 92-93 1.9543154837766679 1104 94-95 1.92918968304798 1104 96-97 1.9668629157119746 1104 98-99 2.064809213408676 1104 100-101 2.047976784928913 1104 102-103 2.053435436739818 1104 104-105 1.8440871868319846 1104 106-107 2.004402676440108 1104 108-109 1.9316656306484639 1104 110-111 2.0260774393624246 1104 112-113 1.975238750546012 1104 114-115 1.823570536729484 1104 116-117 1.892360771731525 1104 118-119 1.8344874194121061 1104 120-121 1.7659253952056133 1104 122-123 1.9254647311544986 1104 124-125 1.6642378778356246 1105 1 0.3860106600915252 1105 2 0.4915278017627642 1105 3 0.39815839831373623 1105 4 0.5436642969567771 1105 5 0.6732398134663242 1105 6 0.7837575034860649 1105 7 1.107872770302297 1105 8 0.9716362932053215 1105 9 1.7403012213119737 1105 10-11 1.2984304635245678 1105 12-13 1.4192129519634307 1105 14-15 1.4260577535873082 1105 16-17 1.3321502641459304 1105 18-19 1.4918333537880244 1105 20-21 1.7007317580933403 1105 22-23 1.6142445469370585 1105 24-25 1.6905907637201452 1105 26-27 1.4115978480310858 1105 28-29 1.4300832646652708 1105 30-31 1.8583408327986604 1105 32-33 1.6185977960877906 1105 34-35 1.796741510284626 1105 36-37 1.5088295661166988 1105 38-39 1.6144541237819467 1105 40-41 1.745006063935044 1105 42-43 1.6715844291034436 1105 44-45 1.5245162090157969 1105 46-47 1.5120145780572223 1105 48-49 1.647618039977047 1105 50-51 1.6458199127920956 1105 52-53 1.583042937907205 1105 54-55 1.7695586327986383 1105 56-57 1.6086596089471712 1105 58-59 1.5124499515017469 1105 60-61 1.4248280549813082 1105 62-63 1.2799699757953569 1105 64-65 1.7231531759976697 1105 66-67 2.0181190220134937 1105 68-69 2.021315743816288 1105 70-71 1.885690505093006 1105 72-73 2.040853851593468 1105 74-75 2.073591864979015 1105 76-77 1.8266269588168917 1105 78-79 2.0072741884948613 1105 80-81 1.9531130386946032 1105 82-83 1.8744570331130106 1105 84-85 2.134039309972156 1105 86-87 2.079488993646013 1105 88-89 1.893101394517167 1105 90-91 1.9968097987023086 1105 92-93 1.8341736515492322 1105 94-95 1.667214227254064 1105 96-97 1.6891911965929118 1105 98-99 1.7093683253049647 1105 100-101 1.7938250588753348 1105 102-103 1.5503336610568539 1105 104-105 1.2389134451456343 1105 106-107 1.438910648705928 1105 108-109 1.452101512109028 1105 110-111 1.6073322590455739 1105 112-113 1.5611672060524455 1105 114-115 1.4177799848624097 1105 116-117 1.450284925701098 1105 118-119 1.5724944816440498 1105 120-121 1.593821854814486 1105 122-123 1.693126259739472 1105 124-125 1.5645503802636753 1106 1 0.46934261039995917 1106 2 0.4080831713205342 1106 3 0.5276518930013623 1106 4 0.36861059295613074 1106 5 0.49419014498675295 1106 6 0.7539255940444605 1106 7 0.9547833942420247 1106 8 0.6469286469909932 1106 9 1.3084430660092963 1106 10-11 0.9366979097597365 1106 12-13 0.8776780626127021 1106 14-15 1.038115744549195 1106 16-17 1.0941220916168035 1106 18-19 1.159557202606976 1106 20-21 0.852022525871817 1106 22-23 0.8435475273453079 1106 24-25 0.6546949348192541 1106 26-27 0.745524145490414 1106 28-29 0.8566736549091374 1106 30-31 1.2525694387033255 1106 32-33 0.9502344594331191 1106 34-35 0.9900736664869996 1106 36-37 0.9461387944699133 1106 38-39 1.2255608223850487 1106 40-41 1.299635370627783 1106 42-43 1.3871425857163437 1106 44-45 1.244204317143808 1106 46-47 1.1761855399000858 1106 48-49 1.1154985553607517 1106 50-51 1.4424793149385096 1106 52-53 1.0241027738035022 1106 54-55 1.5157151546137264 1106 56-57 1.39594544026264 1106 58-59 1.3678553847306567 1106 60-61 1.4875812811208533 1106 62-63 1.4353513568694645 1106 64-65 1.5258716934116165 1106 66-67 1.6960128768283198 1106 68-69 1.6048213001448133 1106 70-71 1.4325337455640152 1106 72-73 1.4287319711205058 1106 74-75 1.6279157623390148 1106 76-77 1.2969731788226717 1106 78-79 1.313151586769223 1106 80-81 1.3490572089950845 1106 82-83 1.23708981130223 1106 84-85 1.4618862147312406 1106 86-87 1.510433915771312 1106 88-89 1.2047399545823545 1106 90-91 1.395632490958885 1106 92-93 1.311639028612774 1106 94-95 1.2736067659075303 1106 96-97 1.2479310508574422 1106 98-99 1.1354177760361068 1106 100-101 0.8854805833526882 1106 102-103 0.7873217572679678 1106 104-105 1.0193034519276551 1106 106-107 0.990572462307032 1106 108-109 0.9290407053916425 1106 110-111 0.8902456519467279 1106 112-113 0.7411500107895037 1106 114-115 0.9009521178050051 1106 116-117 1.1484351558967525 1106 118-119 0.863884309360742 1106 120-121 0.7985350164905114 1106 122-123 1.0661929625103639 1106 124-125 0.9898552748615828 1107 1 0.3769906229406885 1107 2 0.44588550943354477 1107 3 0.21104996322060288 1107 4 0.31752481473869665 1107 5 0.38225559192409975 1107 6 0.758597395415471 1107 7 0.9104448956531677 1107 8 0.7168135112015577 1107 9 1.4524487674906084 1107 10-11 0.8234715160730772 1107 12-13 1.1338404979416623 1107 14-15 0.9166027660940941 1107 16-17 1.0200922757771806 1107 18-19 1.0066998249723298 1107 20-21 1.171502218295231 1107 22-23 0.9782663564557339 1107 24-25 1.4206143618899105 1107 26-27 1.2735878225666042 1107 28-29 0.9438252529989484 1107 30-31 1.423148183302878 1107 32-33 1.2136672315729768 1107 34-35 1.378709577602045 1107 36-37 1.0878493652817518 1107 38-39 1.2669221616642687 1107 40-41 1.4622045453352044 1107 42-43 1.4774184628247689 1107 44-45 1.321560049755714 1107 46-47 1.299093390182474 1107 48-49 1.0817912335083406 1107 50-51 1.5211229375914677 1107 52-53 1.1141098532481486 1107 54-55 1.726942079258727 1107 56-57 1.4545910540094056 1107 58-59 1.185804937366644 1107 60-61 1.59070540211615 1107 62-63 1.4953156752042744 1107 64-65 1.6833569419348855 1107 66-67 1.6093244450833346 1107 68-69 1.6237735107330096 1107 70-71 1.489648271383448 1107 72-73 1.341113256102922 1107 74-75 1.5371087688590563 1107 76-77 1.3600747340881618 1107 78-79 1.457644632163003 1107 80-81 1.3120792785316695 1107 82-83 0.8127970446615862 1107 84-85 1.329504866539498 1107 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-4.2788703690685885 2216 94-95 -3.977819583702775 2216 96-97 -4.245499927322609 2216 98-99 -4.087195254158477 2216 100-101 -4.499295508081762 2216 102-103 -4.52284378988691 2216 104-105 -4.240743401094768 2216 106-107 -4.370310392816215 2216 108-109 -4.495391489671885 2216 110-111 -4.047493437576875 2216 112-113 -4.066216790325548 2216 114-115 -3.9875570294510787 2216 116-117 -3.967957757354384 2216 118-119 -4.171410898777335 2216 120-121 -4.091847191509746 2216 122-123 -4.1689271485019574 2216 124-125 -4.080233889717967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 119535.0 3 38907.0 4 18358.0 5 15578.0 6 16061.0 7 18500.0 8 20565.0 9 22354.0 10 24005.0 11 25482.0 12 28001.0 13 30143.0 14 30998.0 15 25299.0 16 18399.0 17 16401.0 18 14545.0 19 13351.0 20 13346.0 21 14350.0 22 17227.0 23 23475.0 24 32105.0 25 42643.0 26 52126.0 27 59696.0 28 67809.0 29 77177.0 30 86252.0 31 98867.0 32 116149.0 33 138066.0 34 168041.0 35 213288.0 36 298922.0 37 382380.0 38 138686.0 39 645.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.61851694789889 20.325604789001865 14.080770027317824 23.975108235781416 2 15.705787107911922 21.173396507977415 38.70002565287756 24.420790731233108 3 19.235797948719565 25.50805207169236 29.42765429919314 25.828495680394937 4 12.934009478987296 17.546785031632155 36.75870900709069 32.76049648228987 5 13.107964119142604 38.93255867837897 33.92690796348866 14.032569238989776 6 31.37314164480984 37.77560085241602 17.06360965398971 13.787647848784424 7 27.478727744697455 32.91760536591038 21.0041963059105 18.599470583481654 8 25.882953755558113 36.20401996743549 19.283478318435517 18.629547958570882 9 25.65653110730369 16.311139237713043 20.074538997813796 37.95779065716947 10-11 24.85021964632764 27.286386656347677 27.042131586040362 20.82126211128432 12-13 25.63411400969534 24.450726125954304 27.876328853448417 22.038831010901937 14-15 22.499338973306344 25.712795624732287 25.89333176762281 25.89453363433855 16-17 22.066152943733293 29.141957649679156 26.464034501626426 22.327854904961125 18-19 21.936542238446965 27.271043564063035 29.137191017965957 21.65522317952404 20-21 22.681464364428937 26.55430404622865 29.182407955085953 21.58182363425646 22-23 22.283014748881264 26.356157819492104 29.128961683172534 22.2318657484541 24-25 22.053425604118214 26.632273324024027 29.146574711139863 22.1677263607179 26-27 21.938173848638616 26.95680255736143 29.00226610374976 22.102757490250198 28-29 21.846985568892077 26.842960179978036 29.242155023381933 22.067899227747954 30-31 21.850003290369386 26.573161067718953 29.212746615155638 22.364089026756023 32-33 21.81475176634236 26.67407486217998 29.04721936533831 22.463954006139346 34-35 22.26522047523348 26.692418331560074 28.935670094967886 22.106691098238564 36-37 22.337514745146144 26.510723590401465 28.84728865651745 22.304473007934945 38-39 22.037734332315818 26.712824979789897 29.001261173681602 22.248179514212683 40-41 22.16470277624259 26.548468736854446 28.944089955737347 22.342738531165615 42-43 22.23480794480689 26.617857531284134 28.610265641279454 22.537068882629523 44-45 22.289908064552037 26.765586773400923 28.494669099574043 22.449836062472993 46-47 22.4376883574599 27.08454176334107 27.91944211515182 22.558327764047213 48-49 22.547854254872902 27.120264693093027 27.656826579171202 22.675054472862872 50-51 22.443995610830378 27.2464780501112 27.510541753385354 22.79898458567307 52-53 22.772114144485762 27.14608049474117 27.493300117309516 22.58850524346355 54-55 22.393874878688642 27.044398527473927 27.567544212182966 22.99418238165447 56-57 22.400483899409608 27.309304522666526 27.493919240419785 22.796292337504077 58-59 22.495795458306866 26.9862223434153 27.537501989965847 22.980480208311988 60-61 22.620335795899646 27.046945855590742 27.679715588564907 22.653002759944705 62-63 22.486453854442647 27.118141146295223 27.63862916809594 22.75677583116619 64-65 22.254815890244487 26.850831953431033 27.63884997867797 23.25550217764651 66-67 22.17327199148735 27.249899041380914 27.466913263883264 23.109915703248475 68-69 22.370809529579706 26.980713664364366 27.689747810744276 22.95872899531166 70-71 22.249163153081955 27.00397473962628 27.73742877516033 23.009433332131437 72-73 22.413216563963093 27.03708567122474 27.436661528530458 23.113036236281708 74-75 22.330826889891327 27.20067796530019 27.3459928365187 23.122502308289786 76-77 22.42907312336761 27.055496271403374 27.466205406189264 23.049225199039753 78-79 22.45755840884646 27.21749100117883 27.312518237525534 23.01243235244918 80-81 22.215328477939416 27.31593522157127 27.349232728888346 23.11950357160097 82-83 22.4689126516095 27.33544979299401 27.202929706625433 22.992707848771058 84-85 22.24891054252795 27.19338653308802 27.195553823474594 23.362149100909434 86-87 22.400879685891766 27.02204810294579 27.346768206471626 23.23030400469082 88-89 22.349597468563932 27.058998538040502 27.289286713507167 23.3021172798884 90-91 22.228632169198956 27.33933674781226 27.340676531811496 23.091354551177286 92-93 22.333974013538022 27.20070694840833 27.33754520637554 23.127773831678105 94-95 22.194168880678237 27.05308031995259 27.362779227514224 23.389971571854954 96-97 22.38103832419239 26.939262298505795 27.300578563136362 23.379120814165457 98-99 21.985241152336023 26.965317850742316 27.2345149133163 23.81492608360536 100-101 21.98933166357758 26.99917031055526 27.444633351545388 23.56686467432177 102-103 22.281462391523007 26.773377783163248 27.344329543473055 23.600830281840686 104-105 22.140399235142468 26.766053486830245 27.449992168189848 23.643555109837443 106-107 21.922993793076138 26.909358578462133 27.411283428244253 23.75636420021748 108-109 22.150526533140617 26.8358124498568 27.074962998456893 23.938698018545693 110-111 21.90666649591022 26.91307478836873 27.347952055529994 23.83230666019106 112-113 22.08510920004035 26.920320065822658 27.298965846615552 23.695604887521437 114-115 21.908231639619164 27.055319003052535 27.124731526427297 23.911717830901004 116-117 21.77085681230327 26.974164332561518 27.281880040918427 23.97309881421679 118-119 21.784039464229384 27.077592795496535 27.032138079880113 24.106229660393968 120-121 21.72196894854667 27.032040159848115 27.270448866455265 23.975542025149952 122-123 21.858495742695887 27.035117463763903 27.02865491257331 24.077731880966898 124-125 21.674931398717394 27.02596937366034 27.00323035883111 24.295868868791157 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 218.0 1 193.0 2 330.5 3 532.0 4 414.5 5 290.0 6 538.0 7 1205.0 8 1967.0 9 2310.5 10 1956.5 11 1403.5 12 1280.0 13 1420.5 14 1601.5 15 1797.0 16 2048.0 17 2321.5 18 2675.0 19 3096.5 20 3600.5 21 4241.5 22 5063.0 23 6232.0 24 7709.5 25 9924.5 26 13349.0 27 17447.5 28 21624.5 29 26435.5 30 31650.5 31 37295.5 32 43286.5 33 48281.0 34 53392.0 35 57843.0 36 63154.0 37 69208.0 38 72728.5 39 74598.5 40 76221.0 41 78596.0 42 81093.5 43 84016.0 44 87168.0 45 89684.5 46 90070.0 47 90291.5 48 93139.5 49 93901.0 50 91545.5 51 89011.0 52 87040.0 53 86074.0 54 83550.5 55 77541.5 56 70604.0 57 64721.0 58 60605.5 59 55258.0 60 47094.0 61 38247.0 62 30307.5 63 23192.5 64 17181.5 65 13211.0 66 10304.0 67 8239.0 68 6244.0 69 4423.0 70 3201.5 71 2557.0 72 2147.0 73 1835.0 74 1514.0 75 1112.5 76 807.5 77 517.5 78 296.0 79 191.5 80 144.0 81 104.0 82 67.5 83 35.5 84 26.5 85 18.0 86 7.0 87 4.5 88 2.0 89 2.0 90 3.5 91 4.0 92 2.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10864031347675798 2 1.970263211402938E-4 3 0.0 4 0.17929395223766734 5 0.0 6 1.5762105691223503E-4 7 0.038498943150813404 8 0.0 9 0.0 10-11 6.304842276489401E-4 12-13 7.092947561050575E-4 14-15 5.122684349647638E-4 16-17 0.004433092225656609 18-19 0.0017338316260345852 20-21 0.0033494474593849938 22-23 0.0018914526829468203 24-25 0.00506357645330555 26-27 0.004590713282568845 28-29 0.0035070685162972293 30-31 9.063210772453513E-4 32-33 7.881052845611752E-5 34-35 0.0012609684552978802 36-37 0.0012806710874119095 38-39 6.501868597629694E-4 40-41 0.001556507937008321 42-43 9.457263414734101E-4 44-45 0.0010836447662716157 46-47 1.5762105691223503E-4 48-49 1.970263211402938E-5 50-51 0.04072534057969872 52-53 3.940526422805876E-5 54-55 0.13584964842623257 56-57 5.713763313068519E-4 58-59 0.0 60-61 0.0 62-63 6.895921239910282E-4 64-65 0.06393504121002533 66-67 0.031583319278789095 68-69 0.002147586900429202 70-71 0.0032115290345867886 72-73 0.07065363876090935 74-75 0.005477331727700167 76-77 0.0018914526829468203 78-79 0.001517102672780262 80-81 1.7732368902626439E-4 82-83 1.3791842479820562E-4 84-85 3.940526422805876E-5 86-87 3.1524211382447006E-4 88-89 0.0016550210975784677 90-91 3.7435001016655815E-4 92-93 0.00254163954270979 94-95 0.0041769580081742275 96-97 0.002600747439051878 98-99 0.0 100-101 0.0013988868800960859 102-103 0.001556507937008321 104-105 1.7732368902626439E-4 106-107 5.910789634208813E-5 108-109 0.0 110-111 1.7732368902626439E-4 112-113 1.5762105691223503E-4 114-115 2.1672895325432315E-4 116-117 0.0 118-119 0.0016353184654644382 120-121 0.002876584288648289 122-123 0.001359481615868027 124-125 0.009575479207418278 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2537732.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.16539152564142 #Duplication Level Percentage of deduplicated Percentage of total 1 79.53679992504141 47.058259082616814 2 11.33608566830493 13.414078938669475 3 3.8830826280374318 6.89232312042754 4 1.7586811951203183 4.162122459123065 5 0.9408821465926753 2.7833830291320787 6 0.5627607968808929 1.9977577729643998 7 0.38423625944565765 1.5913442109915192 8 0.2666516448279623 1.2621239173762133 9 0.19298253119504688 1.0276098314187834 >10 1.011869467157962 11.003883573369558 >50 0.08385802655791338 3.408326445479158 >100 0.040280049232284836 4.146785635786838 >500 0.0014230701375953995 0.5341666639383282 >1k 2.7106097858862983E-4 0.27343816318011244 >5k 1.3553048929431492E-4 0.44439715552615333 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5875 0.23150592733984518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5209 0.20526202136395807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3168 0.12483587707449012 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.881052845611752E-5 0.0 0.0 0.0 0.0 6 7.881052845611752E-5 0.0 0.0 0.0 0.0 7 7.881052845611752E-5 0.0 0.0 0.0 0.0 8 7.881052845611752E-5 0.0 0.0 0.0 0.0 9 7.881052845611752E-5 0.0 0.0 0.0 0.0 10-11 7.881052845611752E-5 0.0 0.0 0.0 0.0 12-13 7.881052845611752E-5 0.0 0.0 0.0 0.0 14-15 7.881052845611752E-5 0.0 0.0 0.0 0.0 16-17 7.881052845611752E-5 0.0 0.0 3.940526422805876E-5 0.0 18-19 7.881052845611752E-5 0.0 0.0 3.940526422805876E-5 0.0 20-21 7.881052845611752E-5 0.0 0.0 7.881052845611752E-5 0.0 22-23 7.881052845611752E-5 0.0 0.0 2.3643158536835253E-4 0.0 24-25 7.881052845611752E-5 0.0 0.0 4.137552743946169E-4 0.0 26-27 7.881052845611752E-5 0.0 0.0 0.0011033473983856452 0.0 28-29 7.881052845611752E-5 0.0 0.0 0.0016550210975784677 0.0 30-31 7.881052845611752E-5 0.0 0.0 0.0037829053658936405 0.0 32-33 7.881052845611752E-5 0.0 0.0 0.008373618648462484 0.0 34-35 7.881052845611752E-5 0.0 0.0 0.01359481615868027 0.0 36-37 7.881052845611752E-5 0.0 0.0 0.024569182246194633 0.0 38-39 7.881052845611752E-5 0.0 0.0 0.045040217012671156 0.0 40-41 7.881052845611752E-5 0.0 0.0 0.07039750454342697 0.0 42-43 7.881052845611752E-5 0.0 0.0 0.09569568417784069 0.0 44-45 7.881052845611752E-5 0.0 0.0 0.12444182443220955 0.0 46-47 7.881052845611752E-5 0.0 0.0 0.16240879651594414 0.0 48-49 7.881052845611752E-5 0.0 0.0 0.2022081133862835 0.0 50-51 7.881052845611752E-5 0.0 0.0 0.2500658067912609 0.0 52-53 7.881052845611752E-5 0.0 0.0 0.2963669922592299 0.0 54-55 7.881052845611752E-5 1.970263211402938E-5 0.0 0.34520981726990874 0.0 56-57 7.881052845611752E-5 3.940526422805876E-5 0.0 0.4065441110408822 0.0 58-59 7.881052845611752E-5 3.940526422805876E-5 0.0 0.47439997604159934 0.0 60-61 7.881052845611752E-5 3.940526422805876E-5 0.0 0.5542744466318745 0.0 62-63 7.881052845611752E-5 3.940526422805876E-5 0.0 0.633774567211983 1.970263211402938E-5 64-65 9.851316057014688E-5 3.940526422805876E-5 0.0 0.7107330482493818 3.940526422805876E-5 66-67 1.5762105691223503E-4 3.940526422805876E-5 0.0 0.801483371766601 3.940526422805876E-5 68-69 1.5762105691223503E-4 3.940526422805876E-5 0.0 0.9084292588815526 3.940526422805876E-5 70-71 1.5762105691223503E-4 3.940526422805876E-5 0.0 1.0347428333645947 3.940526422805876E-5 72-73 1.7732368902626439E-4 3.940526422805876E-5 0.0 1.1671248185387584 3.940526422805876E-5 74-75 1.9702632114029377E-4 5.910789634208813E-5 0.0 1.297654756294203 3.940526422805876E-5 76-77 1.9702632114029377E-4 7.881052845611752E-5 0.0 1.44193713126524 3.940526422805876E-5 78-79 1.9702632114029377E-4 7.881052845611752E-5 0.0 1.6079712120901655 3.940526422805876E-5 80-81 1.9702632114029377E-4 7.881052845611752E-5 0.0 1.8024164884235216 3.940526422805876E-5 82-83 2.3643158536835253E-4 7.881052845611752E-5 0.0 2.0124465467590746 3.940526422805876E-5 84-85 2.3643158536835253E-4 7.881052845611752E-5 0.0 2.217216002320182 3.940526422805876E-5 86-87 2.3643158536835253E-4 7.881052845611752E-5 0.0 2.4304969949545496 3.940526422805876E-5 88-89 2.3643158536835253E-4 7.881052845611752E-5 0.0 2.6708691067457084 3.940526422805876E-5 90-91 2.3643158536835253E-4 7.881052845611752E-5 0.0 2.9544293881308192 3.940526422805876E-5 92-93 2.3643158536835253E-4 7.881052845611752E-5 0.0 3.261494909627967 3.940526422805876E-5 94-95 2.3643158536835253E-4 7.881052845611752E-5 0.0 3.5677329205763257 3.940526422805876E-5 96-97 2.3643158536835253E-4 7.881052845611752E-5 0.0 3.8755668447259204 3.940526422805876E-5 98-99 2.3643158536835253E-4 7.881052845611752E-5 0.0 4.210334266975394 3.940526422805876E-5 100-101 2.3643158536835253E-4 7.881052845611752E-5 0.0 4.601530027599447 3.940526422805876E-5 102-103 2.3643158536835253E-4 7.881052845611752E-5 0.0 5.03546079727883 3.940526422805876E-5 104-105 2.3643158536835253E-4 7.881052845611752E-5 0.0 5.462318322029276 3.940526422805876E-5 106-107 2.3643158536835253E-4 7.881052845611752E-5 0.0 5.881590333415822 3.940526422805876E-5 108-109 2.3643158536835253E-4 7.881052845611752E-5 0.0 6.32180624273958 3.940526422805876E-5 110-111 2.3643158536835253E-4 7.881052845611752E-5 0.0 6.817051603557823 3.940526422805876E-5 112-113 2.3643158536835253E-4 7.881052845611752E-5 0.0 7.35867696037249 3.940526422805876E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4970 0.0 68.91973 1 GTATCAA 8860 0.0 50.042984 1 ATCAACG 10760 0.0 38.93951 3 TATCAAC 11315 0.0 38.66241 2 TCAACGC 10965 0.0 38.102795 4 CAACGCA 10915 0.0 38.102673 5 AACGCAG 11235 0.0 36.858543 6 GTACATG 10515 0.0 33.25649 1 CGCAGAG 13455 0.0 31.24811 8 TACATGG 10695 0.0 31.04001 2 ACGCAGA 13265 0.0 30.948801 7 GCAGAGT 14435 0.0 27.931929 9 ACATGGG 10745 0.0 27.900309 3 CTACGCG 110 7.678006E-4 27.090984 4 GAGTACT 8650 0.0 26.848415 12-13 TGGTATC 2280 0.0 26.140423 2 CAGAGTA 14545 0.0 25.330355 10-11 GTACTTT 9590 0.0 24.650867 14-15 CATACCG 175 1.7523014E-5 23.79918 5 AGAGTAC 14150 0.0 23.17943 10-11 >>END_MODULE