FastQCFastQC Report
Thu 26 May 2016
SRR1364509_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364509_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408303
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.056.6090771
GTATCAA53300.052.8288461
TATCAAC66900.041.8041572
ATCAACG65250.041.402173
CAACGCA65250.041.2394835
TCAACGC65500.041.172974
AACGCAG65750.039.457496
GTACATG85150.033.627741
ACGCAGA80650.031.429977
TACATGG86900.030.4026152
CGCAGAG80600.030.1950688
ACATGGG89650.027.080563
GCAGAGT87000.026.9478869
GAGTACT38150.025.25918812-13
AGAGTAC79500.023.0114810-11
CAGAGTA86250.022.79706410-11
GAGTACA69050.021.553291
GTATATG12300.020.8113651
GTACTTT44950.020.68200714-15
AGTACTT43250.020.56146212-13
Produced by FastQC (version 0.11.5)