##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364509_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2408303 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.76769534398288 31.0 26.0 31.0 10.0 34.0 2 26.952512619882132 31.0 27.0 31.0 10.0 34.0 3 26.904426062667365 31.0 26.0 31.0 10.0 34.0 4 29.69233688618085 35.0 28.0 37.0 10.0 37.0 5 30.303839674658878 35.0 30.0 37.0 10.0 37.0 6 30.448728835200555 35.0 30.0 37.0 10.0 37.0 7 30.20502777266814 35.0 30.0 37.0 8.0 37.0 8 29.924704241949623 35.0 30.0 37.0 8.0 37.0 9 30.538466297637797 35.0 29.0 39.0 8.0 39.0 10-11 30.698099865340865 36.0 29.0 39.0 7.0 39.0 12-13 30.558019900319852 35.0 29.0 39.0 2.0 39.0 14-15 31.277420241555983 37.0 28.5 40.0 2.0 41.0 16-17 31.54065269195778 37.0 30.0 40.0 2.0 41.0 18-19 31.408712898667652 37.0 29.5 40.0 2.0 41.0 20-21 31.14708095285352 37.0 28.0 40.0 2.0 41.0 22-23 31.203225466230784 37.0 28.5 40.0 2.0 41.0 24-25 31.22644389015834 37.0 29.0 40.0 2.0 41.0 26-27 31.313240692720143 37.0 29.5 40.0 2.0 41.0 28-29 31.257564974174763 37.0 29.5 40.0 2.0 41.0 30-31 30.88291319655376 37.0 29.0 40.0 2.0 41.0 32-33 30.918860708141793 37.0 29.0 40.0 2.0 41.0 34-35 30.605468871649457 36.0 28.0 40.0 2.0 41.0 36-37 30.363155508256227 36.0 27.5 40.0 2.0 41.0 38-39 30.234011044291353 36.0 27.0 40.0 2.0 41.0 40-41 30.281273369671506 36.0 27.5 40.0 2.0 41.0 42-43 30.169394175068504 36.0 27.5 40.0 2.0 41.0 44-45 29.809371993474244 35.5 26.5 40.0 2.0 41.0 46-47 29.56856238604528 35.0 26.5 40.0 2.0 41.0 48-49 29.348458852561325 35.0 26.0 40.0 2.0 41.0 50-51 29.330867212306757 35.0 26.0 40.0 2.0 41.0 52-53 29.17559958194629 35.0 26.0 39.5 2.0 41.0 54-55 29.040548676806864 35.0 26.0 39.0 2.0 41.0 56-57 28.64392395807338 34.5 24.5 39.0 2.0 40.5 58-59 28.332671594894826 34.0 23.5 39.0 2.0 40.0 60-61 27.96070884768237 34.0 23.0 38.5 2.0 40.0 62-63 27.851966716812626 34.0 23.0 38.0 2.0 40.0 64-65 27.625010640272425 33.5 22.5 38.0 2.0 40.0 66-67 27.37854186121929 33.0 22.0 37.0 2.0 40.0 68-69 27.17636755009648 33.0 22.5 37.0 2.0 40.0 70-71 27.043122273235554 33.0 22.5 36.5 2.0 39.0 72-73 27.041875752345113 33.0 23.0 36.0 2.0 39.0 74-75 26.762085377130703 33.0 22.5 36.0 2.0 39.0 76-77 26.474550959742192 33.0 22.0 35.0 2.0 38.5 78-79 26.225251349186543 33.0 21.0 35.0 2.0 37.0 80-81 26.21842538085947 33.0 22.5 35.0 2.0 37.0 82-83 26.059522410593683 33.0 22.0 35.0 2.0 36.0 84-85 25.879801877089385 33.0 21.5 35.0 2.0 36.0 86-87 25.69585430072545 33.0 20.0 35.0 2.0 36.0 88-89 25.50368205329645 33.0 20.0 35.0 2.0 35.0 90-91 25.353947779826708 33.0 19.5 35.0 2.0 35.0 92-93 25.250673814715174 33.0 19.0 35.0 2.0 35.0 94-95 25.21521149954968 33.0 19.0 35.0 2.0 35.0 96-97 25.115454118522464 33.0 18.0 35.0 2.0 35.0 98-99 25.02622489778072 33.0 17.5 35.0 2.0 35.0 100-101 24.95952315800794 33.0 17.5 35.0 2.0 35.0 102-103 23.835561804307844 30.5 10.5 34.0 2.0 35.0 104-105 24.649550534131293 32.0 14.0 35.0 2.0 35.0 106-107 24.70874532814185 32.0 10.0 35.0 2.0 35.0 108-109 24.664899101151306 32.5 8.5 35.0 2.0 35.0 110-111 24.573235386078913 32.0 7.0 35.0 2.0 35.0 112-113 24.54788309444451 32.0 4.5 35.0 2.0 35.0 114-115 24.49839326696018 32.5 2.0 35.0 2.0 35.0 116-117 24.315395114319088 32.0 2.0 35.0 2.0 35.0 118-119 24.18757461166639 32.0 2.0 35.0 2.0 35.0 120-121 24.04591448833473 32.0 2.0 35.0 2.0 35.0 122-123 23.77799035254285 32.0 2.0 35.0 2.0 35.0 124-125 22.749407778008 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6690616608916429 1101 2 1.3548832062787604 1101 3 1.6003792068861955 1101 4 1.927770612212747 1101 5 1.4312137406094685 1101 6 1.3361185816438912 1101 7 1.209136725811316 1101 8 1.5667251544408636 1101 9 2.082257663064471 1101 10-11 2.0270434561536064 1101 12-13 1.8740775408584405 1101 14-15 2.209384446818202 1101 16-17 1.843462266658669 1101 18-19 1.89999291958625 1101 20-21 2.3259234150977264 1101 22-23 2.2043005954790615 1101 24-25 2.0257247985321776 1101 26-27 1.7560359830475285 1101 28-29 1.5677656942976732 1101 30-31 1.8787858675461457 1101 32-33 1.9710731598284816 1101 34-35 2.2373405421275976 1101 36-37 2.2055723147442308 1101 38-39 2.245920440153892 1101 40-41 2.122107189734958 1101 42-43 2.017388946424745 1101 44-45 2.169688393592338 1101 46-47 2.2619102241162494 1101 48-49 2.2094562823986195 1101 50-51 2.045359835624705 1101 52-53 2.0752850516499777 1101 54-55 2.1127045710730954 1101 56-57 2.303578300881476 1101 58-59 2.2312899699642266 1101 60-61 2.48446359901547 1101 62-63 2.2468447526552247 1101 64-65 2.0705930294812553 1101 66-67 2.1744067641784426 1101 68-69 2.0129297151314596 1101 70-71 1.9649657195677186 1101 72-73 1.8144873596456819 1101 74-75 1.8935231240374613 1101 76-77 2.0521114691197617 1101 78-79 2.0460719060383425 1101 80-81 1.8837807541870752 1101 82-83 1.958489924406468 1101 84-85 1.7496665302814343 1101 86-87 1.7505459441391409 1101 88-89 1.8741590709846037 1101 90-91 1.855375505316843 1101 92-93 1.761469723348025 1101 94-95 1.7609044740038797 1101 96-97 1.793284793725725 1101 98-99 1.6624411155224195 1101 100-101 1.6788217017577587 1101 102-103 1.6740669848362764 1101 104-105 1.606612364785203 1101 106-107 1.7227682517868814 1101 108-109 1.8117039916997264 1101 110-111 1.9385773903326644 1101 112-113 1.7968887577394526 1101 114-115 1.789982949430513 1101 116-117 1.9621348491480433 1101 118-119 1.999477464074186 1101 120-121 1.918125131833584 1101 122-123 1.9219088801430964 1101 124-125 1.8964356592836324 1102 1 0.6601459030929213 1102 2 0.39538563488440204 1102 3 0.6302258664379359 1102 4 1.0707034335385792 1102 5 0.8937494025023938 1102 6 0.8231387567366149 1102 7 0.8798422849233205 1102 8 0.8896447900862761 1102 9 1.3505830156311696 1102 10-11 1.070046485764017 1102 12-13 1.4462638766962712 1102 14-15 1.611137354634085 1102 16-17 1.1305337889582248 1102 18-19 1.3346420454885468 1102 20-21 1.2724512921576476 1102 22-23 1.228319842201536 1102 24-25 0.9906069401307214 1102 26-27 1.0509441579417462 1102 28-29 0.8174178115646278 1102 30-31 0.780482658818098 1102 32-33 0.7533595231213006 1102 34-35 1.1678872697932121 1102 36-37 1.3096405877479214 1102 38-39 1.3798478936420402 1102 40-41 0.9503527463974173 1102 42-43 0.9841181532532559 1102 44-45 1.3184763445394196 1102 46-47 1.1592616541433607 1102 48-49 1.2594751605503625 1102 50-51 1.2116849748802423 1102 52-53 1.3068587917481977 1102 54-55 1.0241832841215448 1102 56-57 1.1640179927845757 1102 58-59 1.20761513426163 1102 60-61 1.5827074755964077 1102 62-63 1.3786430499904974 1102 64-65 1.4800389406454677 1102 66-67 1.5049772588509 1102 68-69 1.375757063534838 1102 70-71 1.5580461626903563 1102 72-73 1.3414087593766872 1102 74-75 1.4487591049182242 1102 76-77 1.323543293551701 1102 78-79 1.299308680973894 1102 80-81 0.8805704724068981 1102 82-83 0.774136007949771 1102 84-85 0.8615240755320848 1102 86-87 0.9972546462329106 1102 88-89 0.7260025165267407 1102 90-91 0.6768875604708668 1102 92-93 0.7504155325235438 1102 94-95 0.991040924852161 1102 96-97 1.1019023370549803 1102 98-99 1.1866978900299827 1102 100-101 1.0803465578580962 1102 102-103 0.7732889739162019 1102 104-105 0.7850811298387796 1102 106-107 1.0150792722110253 1102 108-109 1.1736365202633898 1102 110-111 0.9202213899374598 1102 112-113 0.7958698383824583 1102 114-115 0.9165980074684548 1102 116-117 1.1627487049487861 1102 118-119 1.2602347949664647 1102 120-121 1.2861706074059285 1102 122-123 1.35406090521046 1102 124-125 1.2452093136020785 1103 1 1.279568615605914 1103 2 0.9517680386889857 1103 3 1.1406510993950434 1103 4 1.6467210398525687 1103 5 1.1399353910408152 1103 6 1.1122443900211927 1103 7 1.0218094041660848 1103 8 1.412788262228009 1103 9 2.2315862029811164 1103 10-11 1.6003194112269412 1103 12-13 1.8040001568335136 1103 14-15 2.023448206341829 1103 16-17 1.8227052343501313 1103 18-19 1.4656639177001942 1103 20-21 1.9739598791451698 1103 22-23 1.8032037085996038 1103 24-25 1.7846929801818696 1103 26-27 1.5591430177627394 1103 28-29 1.4616117638785617 1103 30-31 1.6602961091589101 1103 32-33 1.7133168871415059 1103 34-35 1.5188524435106814 1103 36-37 1.9163088986824164 1103 38-39 1.9193996064401908 1103 40-41 2.080772103684165 1103 42-43 2.0319252978844347 1103 44-45 1.809335825180753 1103 46-47 2.0807810381063447 1103 48-49 2.0986941103346552 1103 50-51 1.9811276370763409 1103 52-53 2.041707151159848 1103 54-55 1.9836889919156064 1103 56-57 2.056647188542133 1103 58-59 2.2226294267435307 1103 60-61 2.2290674920620823 1103 62-63 2.067738909655109 1103 64-65 1.8823283248844085 1103 66-67 1.903919638186938 1103 68-69 1.7463073643323632 1103 70-71 1.897855036677349 1103 72-73 1.8974185582930794 1103 74-75 1.9074242813953077 1103 76-77 1.9987210009594136 1103 78-79 2.140268657425217 1103 80-81 1.6231760390008922 1103 82-83 1.738018400255978 1103 84-85 1.6604358116949385 1103 86-87 1.699728866872956 1103 88-89 1.8232737218325497 1103 90-91 1.8364592689456316 1103 92-93 1.671572717629008 1103 94-95 1.4643732003348617 1103 96-97 1.6029040756094979 1103 98-99 1.5670737834538322 1103 100-101 1.3654919203329108 1103 102-103 1.44642801918301 1103 104-105 1.6695591265456429 1103 106-107 1.5064822811061696 1103 108-109 1.7391638128096822 1103 110-111 1.8637207253313015 1103 112-113 1.7234035056286388 1103 114-115 1.6252964717452976 1103 116-117 1.6815712022121723 1103 118-119 1.8092862364029365 1103 120-121 1.7894939831452739 1103 122-123 1.8407977579668504 1103 124-125 1.6652861687501321 1104 1 1.1135000393774028 1104 2 0.8979256959942603 1104 3 0.9695424738545846 1104 4 1.1620693831476032 1104 5 0.8506518623294212 1104 6 0.7423838760295958 1104 7 0.7899386183318384 1104 8 1.1632668732822502 1104 9 1.566830651642153 1104 10-11 1.4503117547816267 1104 12-13 0.6998765507365441 1104 14-15 1.1440862933845146 1104 16-17 0.8272845653876502 1104 18-19 0.23479509979432223 1104 20-21 -4.268754486066161 1104 22-23 -1.0307363702839751 1104 24-25 0.4384907989826594 1104 26-27 0.590558467026856 1104 28-29 0.24555519939215387 1104 30-31 0.8988731920865369 1104 32-33 0.7129930694381379 1104 34-35 1.0290727161134257 1104 36-37 1.1375731497960686 1104 38-39 1.158023737669442 1104 40-41 1.269530444632334 1104 42-43 1.196650639553308 1104 44-45 1.3385182000436444 1104 46-47 1.2453887312804 1104 48-49 1.3893802665383461 1104 50-51 1.2092916214904825 1104 52-53 1.274460841649713 1104 54-55 1.1565946351041703 1104 56-57 1.3722256690484365 1104 58-59 1.4005476004261084 1104 60-61 1.4507674670399346 1104 62-63 1.1340755446917825 1104 64-65 1.252113273089666 1104 66-67 1.1356647584513873 1104 68-69 1.0922142453129204 1104 70-71 1.31290763590183 1104 72-73 1.2457655705872241 1104 74-75 1.2718578459199996 1104 76-77 1.2947469452127223 1104 78-79 1.2899218981266571 1104 80-81 0.9786416868055205 1104 82-83 0.9501632701879537 1104 84-85 0.9012121807977422 1104 86-87 0.8664813079197131 1104 88-89 1.0363142571623314 1104 90-91 1.047798464452967 1104 92-93 0.7597686704764008 1104 94-95 0.9197113516805899 1104 96-97 1.0172756395716078 1104 98-99 0.8863576228615564 1104 100-101 0.39246507783431284 1104 102-103 0.828643899871178 1104 104-105 0.9830999107098961 1104 106-107 1.106406354690158 1104 108-109 1.1394704867333267 1104 110-111 1.1269531711272762 1104 112-113 1.0846003911198423 1104 114-115 1.0050597377851354 1104 116-117 1.092733023403106 1104 118-119 1.047860545256171 1104 120-121 0.9989511356244911 1104 122-123 0.7609263585073514 1104 124-125 1.0259191403996475 1105 1 1.0852183829904014 1105 2 0.9104298119989167 1105 3 0.7774265312134041 1105 4 1.4536995052352388 1105 5 0.9439481925328117 1105 6 0.8844902076384109 1105 7 0.9250435010396103 1105 8 1.0730899437818415 1105 9 1.5335845122607275 1105 10-11 1.27915553017489 1105 12-13 1.4152617239525114 1105 14-15 1.653329595629632 1105 16-17 1.4355686854793746 1105 18-19 0.9056225776628288 1105 20-21 1.2213749668442162 1105 22-23 1.2022005365184398 1105 24-25 1.116729528713126 1105 26-27 1.0801649303590501 1105 28-29 0.6870173839961033 1105 30-31 1.026828163248382 1105 32-33 0.9358284217248816 1105 34-35 0.7517658127633347 1105 36-37 1.0729785303339696 1105 38-39 1.0435180609814303 1105 40-41 1.100699348387085 1105 42-43 1.2869205618303354 1105 44-45 1.5832128371549956 1105 46-47 1.6871372527486166 1105 48-49 1.3216676506737244 1105 50-51 1.3775416462904282 1105 52-53 1.324913340961885 1105 54-55 1.3331844352317717 1105 56-57 1.6711806504335946 1105 58-59 1.4473650960775508 1105 60-61 1.8133684629248812 1105 62-63 1.5344243055202007 1105 64-65 1.331034369266387 1105 66-67 1.568972142184574 1105 68-69 1.1113316896568435 1105 70-71 1.243880033084288 1105 72-73 1.2220341004722535 1105 74-75 1.52951330563236 1105 76-77 1.3410281666036141 1105 78-79 1.2815465659112988 1105 80-81 1.25314071946719 1105 82-83 1.324891552091497 1105 84-85 1.2692716364077476 1105 86-87 1.2793187936197228 1105 88-89 1.4186402539432237 1105 90-91 1.3973284282366158 1105 92-93 1.3400145275372601 1105 94-95 1.199176973787381 1105 96-97 1.4142033840437485 1105 98-99 1.246667671058269 1105 100-101 1.2109497219211036 1105 102-103 1.29743068201104 1105 104-105 1.4293001769877307 1105 106-107 1.5323020371852358 1105 108-109 1.5965639397834614 1105 110-111 1.504419782760344 1105 112-113 1.4983952727030498 1105 114-115 1.4681684556936396 1105 116-117 1.6773791436186407 1105 118-119 1.760452199734445 1105 120-121 1.6982723754218298 1105 122-123 1.7640498597676846 1105 124-125 1.4465546503949511 1106 1 -0.3379361159592875 1106 2 -0.20456196023539874 1106 3 -0.16485433704178476 1106 4 -0.4778857310344016 1106 5 -0.9342500588526548 1106 6 -0.4915355984903158 1106 7 -0.5634641569689407 1106 8 -0.05684573089262557 1106 9 0.11922394473720743 1106 10-11 -2.5277924367954157 1106 12-13 -0.5382052331458667 1106 14-15 -0.09670614452404536 1106 16-17 -0.3232463308996927 1106 18-19 -3.0361258955317076 1106 20-21 -5.674642850172685 1106 22-23 -6.337183432985764 1106 24-25 -6.328316306730631 1106 26-27 -5.221498243774388 1106 28-29 -1.8523776493158088 1106 30-31 -3.6065827055582638 1106 32-33 -4.135317538513995 1106 34-35 -3.502505338340704 1106 36-37 -6.0482992224456495 1106 38-39 -6.404630680154202 1106 40-41 -2.5659063032144225 1106 42-43 -1.0519962485306706 1106 44-45 -0.41574004006060505 1106 46-47 -0.17188151146924469 1106 48-49 0.046359509147812616 1106 50-51 -0.1337527636019864 1106 52-53 -0.2883198651048353 1106 54-55 -0.20800988601115478 1106 56-57 -0.03222745415889605 1106 58-59 0.058323965590425075 1106 60-61 0.3491645148948912 1106 62-63 -0.009656395167535692 1106 64-65 -0.17701620171877153 1106 66-67 -0.504093070099195 1106 68-69 -0.39367876708957894 1106 70-71 -0.13971822534494294 1106 72-73 -0.21889275466763536 1106 74-75 -0.37087911777944527 1106 76-77 -0.31276154088089925 1106 78-79 -0.27183671172841173 1106 80-81 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24-25 0.6734277984347337 2211 26-27 0.5259102595897822 2211 28-29 0.5920759555164032 2211 30-31 0.8545592005802654 2211 32-33 0.5133661054232057 2211 34-35 0.651586267010444 2211 36-37 0.8232005885223117 2211 38-39 0.7328410244644097 2211 40-41 0.5650053891740292 2211 42-43 0.4999633856854544 2211 44-45 0.6010887842351771 2211 46-47 0.7260089422752003 2211 48-49 0.5615081251105245 2211 50-51 0.2561246583162742 2211 52-53 0.2736991259219579 2211 54-55 0.339168932792564 2211 56-57 0.7421627767754551 2211 58-59 0.8347950189179763 2211 60-61 1.0437811614239934 2211 62-63 0.8393777837265795 2211 64-65 0.5377450549452014 2211 66-67 0.369758851134371 2211 68-69 0.22038811897602173 2211 70-71 0.6247943622371892 2211 72-73 0.7977579841836686 2211 74-75 0.8881823162568807 2211 76-77 0.969561811744299 2211 78-79 0.9401898042997807 2211 80-81 0.6046772668610707 2211 82-83 0.4985117012537259 2211 84-85 0.3736294579844426 2211 86-87 0.5349483816314162 2211 88-89 0.6687457926726594 2211 90-91 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68-69 -1.7238058888549155 2216 70-71 -4.070165450195258 2216 72-73 -4.326678969324902 2216 74-75 -2.046849026600544 2216 76-77 -1.433730049602005 2216 78-79 -1.7262587551534523 2216 80-81 -1.9658374322214165 2216 82-83 -2.0915772717040007 2216 84-85 -1.8519182521897086 2216 86-87 -2.019582861982812 2216 88-89 -2.0663580681597793 2216 90-91 -2.201809515046591 2216 92-93 -2.1103872118955387 2216 94-95 -5.411060987915231 2216 96-97 -8.220184992147217 2216 98-99 -4.189693467658991 2216 100-101 -2.9926430572575775 2216 102-103 -4.0833350383596105 2216 104-105 -7.010472168944769 2216 106-107 -3.3161625514380795 2216 108-109 -6.404002721811192 2216 110-111 -3.4585502964224446 2216 112-113 -2.657642947697955 2216 114-115 -2.875419446087772 2216 116-117 -2.352996217051711 2216 118-119 -2.6510015979057506 2216 120-121 -3.4554565730418076 2216 122-123 -6.958581754395059 2216 124-125 -5.146717176409908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 212214.0 3 31924.0 4 19002.0 5 16075.0 6 15926.0 7 17057.0 8 18672.0 9 19355.0 10 20732.0 11 21382.0 12 21445.0 13 21542.0 14 21236.0 15 18285.0 16 14919.0 17 13481.0 18 12485.0 19 12045.0 20 12267.0 21 13688.0 22 16784.0 23 23231.0 24 31017.0 25 39768.0 26 48475.0 27 55655.0 28 64514.0 29 73049.0 30 83090.0 31 95941.0 32 114937.0 33 141610.0 34 176139.0 35 226611.0 36 299076.0 37 290904.0 38 73581.0 39 189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24769640218166 18.186162953259778 13.58129460285691 23.984846041701648 2 15.395715008435907 19.589244905435617 40.17925157607309 24.835788510055384 3 19.267250546660552 24.598852511315375 28.244114675758848 27.88978226626523 4 11.804131869884857 15.978291710130188 36.9433916959643 35.27418472402065 5 14.424024434219207 37.618627436317986 33.02682546250368 14.930522666959135 6 33.088394674577344 35.67465956211679 16.855505846221817 14.381439917084057 7 29.25768888516096 32.07641734720953 19.970816971168677 18.69507679646083 8 26.426771960231722 35.45452695753059 19.140654358702644 18.978046723535048 9 26.15846313111746 16.607513841428904 19.657590476415898 37.57643255103773 10-11 25.25611182675832 26.526957287041075 26.60105325086696 21.615877635333643 12-13 25.9376583803487 23.65777034104577 27.161826227546975 23.242745051058556 14-15 23.02527324673599 24.987272017540946 24.75812680641839 27.229327929304674 16-17 23.375952143337418 28.35689577910981 24.89051467757429 23.376637399978488 18-19 23.32772259793246 26.97872904176447 26.863773337918275 22.829775022384798 20-21 23.918947404746174 26.23262135583256 27.00801063039383 22.840420609027436 22-23 23.696768179857816 26.057368799514443 26.784648246960636 23.461214773667106 24-25 23.424687059534893 26.187080714594806 26.773322380797737 23.614909845072564 26-27 23.385286887892317 26.549175101411066 26.553597593499887 23.511940417196726 28-29 23.418105095835646 26.47290323129841 26.666781551371216 23.44221012149473 30-31 23.388986942644678 26.24005012748834 26.80163827859913 23.569324651267856 32-33 23.371226686576733 26.405241840042322 26.48015374380959 23.74337772957135 34-35 23.586974149597147 26.190308585563155 26.680533674215027 23.542183590624674 36-37 23.653120481014682 26.038096246536636 26.63243008127296 23.67635319117572 38-39 23.469020161691354 26.101607217122663 26.714080128931172 23.715292492254807 40-41 23.600905827651836 26.150519081445566 26.65014068407267 23.598434406829927 42-43 23.577019015161756 26.23129697493708 26.422970602776285 23.768713407124878 44-45 23.35953346855984 26.209930834968244 26.59985701459781 23.830678681874108 46-47 23.520259278906494 26.499952765894275 26.25028677849903 23.729501176700193 48-49 23.685857386618693 26.398254883224926 26.01245135669707 23.903436373459307 50-51 23.589308724835 26.625470244267476 25.972225740503653 23.812995290393875 52-53 23.933427185594923 26.385732795136292 25.822536170495507 23.858303848773275 54-55 23.739741818994233 26.35089062729696 25.851799651007983 24.05756790270083 56-57 23.432413128695767 26.580750448475182 25.947133911368013 24.039702511461034 58-59 23.73350382187242 26.296147562564837 25.952831042756962 24.017517572805776 60-61 23.809526776159863 26.14168244397886 26.169987118569587 23.878803661291688 62-63 23.63324353364374 26.372414365874043 26.020980944817314 23.973361155664907 64-65 23.569353327377172 26.282036774694937 26.041437194228518 24.107172703699376 66-67 23.606229454825115 26.489135869321473 25.82967624375651 24.0749584320969 68-69 23.4811523488191 26.274736568907347 26.097482481183494 24.146628601090057 70-71 23.353281104955535 26.168323259788252 26.342789267776357 24.135606367479852 72-73 23.65008218502333 26.33309049113986 25.8780157086857 24.13881161515111 74-75 23.422916163194056 26.491545397243005 25.96243201562613 24.12310642393681 76-77 23.690819253938635 26.3181608567254 25.987983469952137 24.003036419383832 78-79 23.646330713860777 26.340099525421756 25.99658835243171 24.016981408285755 80-81 23.460834627156714 26.583741550354056 25.944954753207426 24.010469069281804 82-83 23.624624563522005 26.600668466906985 25.856584822587187 23.918122146983823 84-85 23.622906824602 26.380103668940386 25.763492305386126 24.233497201071486 86-87 23.565259529536924 26.216820452258784 26.043809065854784 24.174110952349512 88-89 23.528856059998912 26.329605340471236 25.99242082288802 24.14911777664183 90-91 23.439819549796344 26.50312536463972 26.00956150218483 24.047493583379115 92-93 23.570426507773607 26.35089584965314 25.88771613513495 24.190961507438303 94-95 23.488955946282296 26.22359096078887 25.97564839410369 24.31180469882515 96-97 23.49663253910434 26.262769288391226 25.975446674654425 24.265151497850013 98-99 23.477920733513866 26.169861976166448 25.982443220546426 24.36977406977326 100-101 23.318797818904642 26.269397209040047 25.970153716023674 24.441651256031633 102-103 23.401926926604798 25.95367420299291 26.201318694460095 24.443080175942193 104-105 23.40959287321547 26.081618026572635 26.128477448721426 24.38031165149047 106-107 23.20068496890629 26.177326640602473 26.245632523898237 24.376355866593006 108-109 23.382379504001825 26.03037443813518 26.02132231576543 24.56592374209756 110-111 23.202558538888933 26.20062829868749 26.19940334255234 24.39740981987124 112-113 23.277186559987243 26.22057360398503 26.10623566363759 24.396004172390136 114-115 23.20949006237113 26.260341813158167 26.11727085133139 24.412897273139304 116-117 22.941907798764962 26.346146419570886 26.30744513823458 24.404500643429564 118-119 23.172231143046137 26.25778426665637 25.99588991551032 24.57409467478718 120-121 23.13898619130443 26.253526296982777 26.240175835748214 24.367311675964583 122-123 23.120037006908483 26.243761416726773 26.239837322413972 24.396364253950775 124-125 23.244044373403792 26.322077959845412 25.80217255608213 24.631705110668666 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1042.0 1 1010.5 2 1187.5 3 1282.5 4 1027.0 5 770.5 6 685.0 7 821.0 8 1064.5 9 1215.5 10 1113.0 11 1022.0 12 1057.5 13 1114.5 14 1235.5 15 1368.5 16 1492.0 17 1623.5 18 1803.5 19 2076.0 20 2453.0 21 2962.0 22 3716.0 23 4851.0 24 6299.5 25 8019.0 26 10262.0 27 12953.0 28 16109.5 29 19316.5 30 22735.0 31 26606.0 32 30093.5 33 32995.0 34 35580.0 35 38458.5 36 42155.5 37 46489.5 38 50020.0 39 52415.0 40 55769.5 41 60167.0 42 64497.0 43 69183.5 44 73176.0 45 77183.0 46 80932.5 47 83860.0 48 88180.5 49 91604.0 50 92105.0 51 92803.0 52 95088.0 53 96818.0 54 95860.0 55 91797.5 56 86843.5 57 81755.5 58 77519.5 59 71545.5 60 61731.0 61 50641.5 62 40200.0 63 31393.5 64 24232.5 65 18846.0 66 14887.0 67 11859.5 68 9263.5 69 7153.5 70 5425.0 71 4087.0 72 3203.0 73 2682.0 74 2202.0 75 1737.5 76 1275.5 77 852.5 78 554.5 79 369.0 80 255.5 81 184.0 82 145.5 83 103.0 84 71.0 85 57.0 86 47.0 87 40.0 88 26.5 89 20.5 90 21.0 91 13.5 92 9.5 93 10.0 94 8.5 95 5.5 96 4.0 97 4.0 98 3.0 99 2.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08582807063729107 2 0.030602461567336002 3 0.039820570750441285 4 0.046173591944203035 5 0.04928781801957644 6 0.0033633641614032785 7 0.0022422427742688524 8 0.002159196745592228 9 0.005564083921333819 10-11 0.025515892310892775 12-13 0.005667891457179599 14-15 0.009259632197443595 16-17 0.01851926439488719 18-19 0.01739814300775276 20-21 0.12971789679288695 22-23 0.014802954611608257 24-25 0.013993255832011171 26-27 0.006581397772622465 28-29 0.0037993558119555557 30-31 0.0018062511237165754 32-33 0.005854745021702004 34-35 0.01783413465830504 36-37 0.002512142367467881 38-39 0.008159272317478324 40-41 0.03242947419822174 42-43 0.012602234851677717 44-45 0.10227118431526266 46-47 0.0035502177259256825 48-49 0.008802879039722161 50-51 0.010214661527224772 52-53 0.010920552770976076 54-55 0.009342678226120218 56-57 0.005937791050378628 58-59 0.013328887602598177 60-61 0.023813448723021977 62-63 0.006872058872990649 64-65 0.006332259686592592 66-67 0.008096987795970855 68-69 0.007391096552219551 70-71 0.004048493897985428 72-73 7.266527509204614E-4 74-75 0.0012041674158110502 76-77 0.008449933417846509 78-79 0.015591891884036188 80-81 0.0027405189463285975 82-83 0.0044014395198610804 84-85 0.0024913808602987247 86-87 0.003321841147064967 88-89 0.005231899806627322 90-91 0.004318393491184456 92-93 0.0024290963387912567 94-95 0.003487933204418215 96-97 0.0014325439946717667 98-99 0.002470619353129569 100-101 0.0026367114104828174 102-103 4.98276172059745E-4 104-105 0.001494828516179235 106-107 7.681757652587736E-4 108-109 0.001162644401472738 110-111 0.002221481267099696 112-113 0.0037785943047863994 114-115 0.0023875733244529446 116-117 0.0047336236345675775 118-119 0.003321841147064967 120-121 0.00608312160056272 122-123 0.004318393491184456 124-125 0.05109406914329302 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2408303.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.833644663003994 #Duplication Level Percentage of deduplicated Percentage of total 1 80.10266596015853 48.72937117579589 2 10.514653533958413 12.792893936788504 3 3.7526891593130336 6.848692765450688 4 1.8524346397064764 4.507614025333744 5 0.9846483612133669 2.994987426203158 6 0.644054190888575 2.3508098275340465 7 0.44339558173603744 1.888135848513323 8 0.2962998305686751 1.4419998885218455 9 0.216083603156331 1.1830637818722427 >10 1.105044978848519 11.767157146663322 >50 0.061535874132343675 2.5507709143241266 >100 0.02557259138799112 2.4406921907984245 >500 6.380964912508116E-4 0.23014603897509103 >1k 2.835984405549051E-4 0.2736650332257278 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.076150716915604E-5 0.0 18-19 0.0 0.0 0.0 1.2456904301493625E-4 0.0 20-21 0.0 0.0 0.0 1.6609205735324833E-4 2.076150716915604E-5 22-23 0.0 0.0 0.0 2.906611003681846E-4 4.152301433831208E-5 24-25 0.0 0.0 0.0 5.605606935672131E-4 4.152301433831208E-5 26-27 0.0 0.0 0.0 7.681757652587735E-4 4.152301433831208E-5 28-29 0.0 0.0 0.0 0.001474067009010079 4.152301433831208E-5 30-31 0.0 0.0 0.0 0.003591740740263995 4.152301433831208E-5 32-33 0.0 0.0 0.0 0.007183481480527989 4.152301433831208E-5 34-35 0.0 0.0 0.0 0.012643757866016028 4.152301433831208E-5 36-37 0.0 0.0 0.0 0.021944913077797935 4.152301433831208E-5 38-39 0.0 0.0 0.0 0.03708005180411268 4.152301433831208E-5 40-41 0.0 0.0 0.0 0.055910738806537214 8.304602867662416E-5 42-43 0.0 0.0 0.0 0.07625701583231013 8.304602867662416E-5 44-45 0.0 0.0 0.0 0.09805659835992397 8.304602867662416E-5 46-47 0.0 0.0 0.0 0.12681128578920509 8.304602867662416E-5 48-49 0.0 0.0 0.0 0.1610470111111434 8.304602867662416E-5 50-51 0.0 0.0 0.0 0.1999540755461418 8.304602867662416E-5 52-53 0.0 0.0 0.0 0.239629315746399 8.304602867662416E-5 54-55 0.0 0.0 0.0 0.2825848740793828 8.304602867662416E-5 56-57 0.0 0.0 0.0 0.3349246336528252 8.304602867662416E-5 58-59 0.0 0.0 0.0 0.39330599181249204 8.304602867662416E-5 60-61 0.0 0.0 0.0 0.4587047393953335 8.304602867662416E-5 62-63 0.0 0.0 0.0 0.5270723825033644 8.304602867662416E-5 64-65 0.0 0.0 0.0 0.6004020258248235 8.304602867662416E-5 66-67 0.0 0.0 0.0 0.6832404394297562 8.304602867662416E-5 68-69 0.0 0.0 0.0 0.7821067365692772 8.304602867662416E-5 70-71 0.0 0.0 4.152301433831208E-5 0.8971877708079092 8.304602867662416E-5 72-73 0.0 0.0 4.152301433831208E-5 1.0203450313353428 8.304602867662416E-5 74-75 0.0 0.0 4.152301433831208E-5 1.146512710402304 8.304602867662416E-5 76-77 0.0 0.0 4.152301433831208E-5 1.2848466326703907 8.304602867662416E-5 78-79 0.0 0.0 4.152301433831208E-5 1.4433192168925588 8.304602867662416E-5 80-81 0.0 0.0 4.152301433831208E-5 1.626664086703376 8.304602867662416E-5 82-83 0.0 0.0 4.152301433831208E-5 1.8227772834232239 8.304602867662416E-5 84-85 0.0 0.0 4.152301433831208E-5 2.018018496841967 8.304602867662416E-5 86-87 0.0 0.0 4.152301433831208E-5 2.2255089994905126 8.304602867662416E-5 88-89 0.0 0.0 4.152301433831208E-5 2.459179762679364 8.304602867662416E-5 90-91 0.0 0.0 4.152301433831208E-5 2.7278751884625816 8.304602867662416E-5 92-93 0.0 0.0 4.152301433831208E-5 3.0215674688774627 8.304602867662416E-5 94-95 0.0 0.0 4.152301433831208E-5 3.31880996701827 8.304602867662416E-5 96-97 0.0 0.0 4.152301433831208E-5 3.6211805574298586 8.304602867662416E-5 98-99 0.0 0.0 4.152301433831208E-5 3.941219190442399 8.304602867662416E-5 100-101 2.076150716915604E-5 0.0 4.152301433831208E-5 4.30969441968058 1.2456904301493625E-4 102-103 4.152301433831208E-5 0.0 4.152301433831208E-5 4.710765215174336 1.2456904301493625E-4 104-105 4.152301433831208E-5 0.0 4.152301433831208E-5 5.115199374829496 1.2456904301493625E-4 106-107 4.152301433831208E-5 0.0 4.152301433831208E-5 5.51558504058667 1.2456904301493625E-4 108-109 4.152301433831208E-5 0.0 4.152301433831208E-5 5.938040188464658 1.2456904301493625E-4 110-111 4.152301433831208E-5 0.0 4.152301433831208E-5 6.405963037043096 1.2456904301493625E-4 112-113 4.152301433831208E-5 2.076150716915604E-5 4.152301433831208E-5 6.909657962473991 1.2456904301493625E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2955 0.0 56.609077 1 GTATCAA 5330 0.0 52.828846 1 TATCAAC 6690 0.0 41.804157 2 ATCAACG 6525 0.0 41.40217 3 CAACGCA 6525 0.0 41.239483 5 TCAACGC 6550 0.0 41.17297 4 AACGCAG 6575 0.0 39.45749 6 GTACATG 8515 0.0 33.62774 1 ACGCAGA 8065 0.0 31.42997 7 TACATGG 8690 0.0 30.402615 2 CGCAGAG 8060 0.0 30.195068 8 ACATGGG 8965 0.0 27.08056 3 GCAGAGT 8700 0.0 26.947886 9 GAGTACT 3815 0.0 25.259188 12-13 AGAGTAC 7950 0.0 23.01148 10-11 CAGAGTA 8625 0.0 22.797064 10-11 GAGTACA 6905 0.0 21.55329 1 GTATATG 1230 0.0 20.811365 1 GTACTTT 4495 0.0 20.682007 14-15 AGTACTT 4325 0.0 20.561462 12-13 >>END_MODULE