FastQCFastQC Report
Thu 26 May 2016
SRR1364509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364509_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408303
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55370.22991293039123398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52150.2165425197742975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.13420238234142465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41000.067.3470461
GTATCAA72550.051.129841
TATCAAC98450.037.7621352
ATCAACG97550.036.7668883
TCAACGC97100.036.691854
CAACGCA97550.036.407755
AACGCAG98500.035.3318526
GTGGTAT20650.028.880281
GTACATG82300.028.8406281
ACGCAGA119500.028.6748627
CGCAGAG122800.028.3320528
TGGTATC20700.027.342672
GAGTACT74800.026.79580712-13
GCAGAGT127900.025.9468239
TACATGG89050.025.4905192
GTACTTT82100.024.66678414-15
AGTACTT78550.024.41867412-13
AGAGTAC116750.024.17229510-11
ACATGGG88650.023.8581773
CAGAGTA125300.023.40110-11
Produced by FastQC (version 0.11.5)