##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364509_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2408303 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.318267261220868 31.0 28.0 31.0 22.0 34.0 2 28.55749671033919 31.0 28.0 33.0 22.0 34.0 3 28.622380987774378 31.0 28.0 33.0 23.0 34.0 4 31.982344829533492 35.0 32.0 37.0 24.0 37.0 5 31.08584758645403 35.0 31.0 37.0 16.0 37.0 6 31.091244332627582 35.0 31.0 37.0 17.0 37.0 7 30.635848147014723 35.0 30.0 37.0 11.0 37.0 8 30.663190221496215 35.0 30.0 37.0 11.0 37.0 9 31.252878063931323 37.0 30.0 39.0 10.0 39.0 10-11 31.365195949180816 37.0 30.0 39.0 10.0 39.0 12-13 31.13404604819244 37.0 30.0 39.0 8.0 39.0 14-15 31.979809226662923 37.0 30.0 40.0 8.0 41.0 16-17 32.00436220027131 37.0 30.0 40.0 8.0 41.0 18-19 31.852048724765943 37.0 30.0 40.0 7.5 41.0 20-21 31.768516876821565 37.0 30.0 40.0 4.5 41.0 22-23 31.70538466297638 37.0 30.0 40.0 2.0 41.0 24-25 31.517876695748 37.0 29.5 40.0 2.0 41.0 26-27 31.550767490635522 37.0 30.0 40.0 2.0 41.0 28-29 31.406166499813352 37.0 30.0 40.0 2.0 41.0 30-31 30.934026366283646 36.5 28.5 40.0 2.0 41.0 32-33 30.837847438632096 36.0 29.0 40.0 2.0 41.0 34-35 30.64107859351585 36.0 28.0 40.0 2.0 41.0 36-37 30.577711774639653 36.0 28.0 40.0 2.0 41.0 38-39 30.347849709940984 36.0 28.0 40.0 2.0 41.0 40-41 30.09009767458663 35.5 27.0 40.0 2.0 41.0 42-43 29.94538996961761 35.0 26.5 40.0 2.0 41.0 44-45 29.826909653810173 35.0 27.0 40.0 2.0 41.0 46-47 29.656158299018024 35.0 26.5 40.0 2.0 41.0 48-49 29.390273358460295 35.0 26.0 40.0 2.0 41.0 50-51 29.05315444111476 35.0 24.0 40.0 2.0 41.0 52-53 28.917673149931716 35.0 24.0 39.0 2.0 41.0 54-55 28.44458089368323 34.5 23.0 39.0 2.0 41.0 56-57 28.40486745230978 34.0 23.0 39.0 2.0 41.0 58-59 28.147822553889604 34.0 23.0 39.0 2.0 41.0 60-61 27.846538205533108 34.0 22.5 38.5 2.0 40.0 62-63 27.569748283334782 34.0 22.0 38.0 2.0 40.0 64-65 27.140089722929382 33.5 21.0 37.5 2.0 40.0 66-67 26.75914056495383 33.0 19.5 37.0 2.0 40.0 68-69 26.573437395543667 33.0 19.5 37.0 2.0 39.5 70-71 26.59448001351989 33.0 20.5 36.0 2.0 39.0 72-73 26.385978010242066 33.0 19.5 36.0 2.0 39.0 74-75 26.07226084093239 33.0 19.0 35.5 2.0 39.0 76-77 25.972285256464822 33.0 19.5 35.0 2.0 37.5 78-79 25.630895697094594 33.0 18.0 35.0 2.0 37.0 80-81 25.41813197924015 33.0 17.5 35.0 2.0 36.5 82-83 25.30521450166362 32.5 18.0 35.0 2.0 36.0 84-85 24.987310359203143 32.0 12.0 35.0 2.0 36.0 86-87 24.857827067441264 32.0 11.5 35.0 2.0 35.5 88-89 24.788044735234728 32.0 12.0 35.0 2.0 35.0 90-91 24.63903005560347 32.0 10.5 35.0 2.0 35.0 92-93 24.653388506346587 32.0 10.5 35.0 2.0 35.0 94-95 24.65250178237539 32.0 10.0 35.0 2.0 35.0 96-97 24.55043094660431 32.0 7.5 35.0 2.0 35.0 98-99 24.439106084242724 32.0 7.0 35.0 2.0 35.0 100-101 24.34095066110867 32.0 7.0 35.0 2.0 35.0 102-103 23.231715859673802 30.0 7.0 34.0 2.0 35.0 104-105 24.138792959191598 32.0 6.0 34.5 2.0 35.0 106-107 24.25917046152415 32.0 2.0 35.0 2.0 35.0 108-109 24.316703296885816 32.0 2.0 35.0 2.0 35.0 110-111 24.256594581329672 32.0 2.0 35.0 2.0 35.0 112-113 24.19829045597668 32.0 2.0 35.0 2.0 35.0 114-115 24.13940334750237 32.0 2.0 35.0 2.0 35.0 116-117 24.051458433594114 32.0 2.0 35.0 2.0 35.0 118-119 23.932138314821678 32.0 2.0 35.0 2.0 35.0 120-121 23.763774740969055 32.0 2.0 35.0 2.0 35.0 122-123 23.4634134492213 32.0 2.0 35.0 2.0 35.0 124-125 22.703190586898742 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3756411598542293 1101 2 1.375537249956274 1101 3 1.3976363587231866 1101 4 1.4774000450239875 1101 5 1.7167980270973935 1101 6 1.6748450047034886 1101 7 2.105020406010034 1101 8 2.043865836591614 1101 9 2.673806141351001 1101 10-11 2.4830572033086185 1101 12-13 2.6281973537115313 1101 14-15 2.5795474496668405 1101 16-17 2.5211510512604036 1101 18-19 2.642581955228721 1101 20-21 2.793055305150716 1101 22-23 2.830377527799591 1101 24-25 3.02624968849506 1101 26-27 2.706747761395551 1101 28-29 2.8550713327859434 1101 30-31 3.236263748967559 1101 32-33 3.0793870351511075 1101 34-35 3.1636815228944286 1101 36-37 3.0421435107800328 1101 38-39 3.0765371235412857 1101 40-41 3.3021062977999804 1101 42-43 3.330732003283316 1101 44-45 3.0998478954788915 1101 46-47 2.943813831776666 1101 48-49 2.9820788176357134 1101 50-51 3.2343078829570047 1101 52-53 3.0586044417525677 1101 54-55 3.3406305331730373 1101 56-57 3.1059150839407508 1101 58-59 3.1875442821102737 1101 60-61 3.303307661702352 1101 62-63 3.3470264954641245 1101 64-65 3.3922715741233596 1101 66-67 3.61188364974549 1101 68-69 3.4868341059254746 1101 70-71 3.071753431669066 1101 72-73 3.3423757483692498 1101 74-75 3.3573504373826175 1101 76-77 3.1731430537814482 1101 78-79 3.259047378741549 1101 80-81 3.191688809920688 1101 82-83 3.145053961151074 1101 84-85 3.4566240916250948 1101 86-87 3.2970551584208003 1101 88-89 3.114887859889503 1101 90-91 3.1919978653351073 1101 92-93 3.1018923915068406 1101 94-95 3.103265189345084 1101 96-97 3.099554663454903 1101 98-99 3.1619190831025 1101 100-101 3.260276566917998 1101 102-103 2.9562786520105035 1101 104-105 3.0365865669840275 1101 106-107 3.12758772587409 1101 108-109 3.0676923503560403 1101 110-111 3.0789521055981233 1101 112-113 2.9595150899985967 1101 114-115 2.9501258215997552 1101 116-117 2.9402722792160354 1101 118-119 2.8622888140482736 1101 120-121 2.824941605603801 1101 122-123 2.9775649829097546 1101 124-125 2.7281206259858024 1102 1 0.9322856318396688 1102 2 -1.2230436958249022 1102 3 0.4825583897368553 1102 4 1.0124195571902384 1102 5 1.5870646023081356 1102 6 1.0113779106252991 1102 7 1.9278560944928742 1102 8 1.664649469410481 1102 9 2.4469289462038404 1102 10-11 2.1619437406651656 1102 12-13 2.4188461918600375 1102 14-15 2.4634893306842898 1102 16-17 2.416687572089316 1102 18-19 2.501126490137537 1102 20-21 2.7551771502171505 1102 22-23 2.6356407701657005 1102 24-25 2.8522744016291064 1102 26-27 2.516898151456747 1102 28-29 2.5818718372201808 1102 30-31 2.76714739785875 1102 32-33 2.6444730915217107 1102 34-35 2.8238896458381433 1102 36-37 2.21514572197675 1102 38-39 2.149570329737827 1102 40-41 2.3685760595198033 1102 42-43 2.1870867408855403 1102 44-45 2.1471740901352305 1102 46-47 1.8195092557627675 1102 48-49 1.8347905614055264 1102 50-51 2.0424811608279185 1102 52-53 1.9687018967250154 1102 54-55 2.2430768445517835 1102 56-57 1.806242153468805 1102 58-59 1.9695645471260583 1102 60-61 1.8814976994108505 1102 62-63 2.173771816733044 1102 64-65 2.1891853178731395 1102 66-67 2.398767215770871 1102 68-69 2.3267626318884247 1102 70-71 1.67759959174942 1102 72-73 1.8377944244559075 1102 74-75 2.1149923325600994 1102 76-77 1.9890206322968034 1102 78-79 1.9228772383887218 1102 80-81 2.0316895589001156 1102 82-83 1.7536603608502048 1102 84-85 1.7527687370823664 1102 86-87 1.777409729608447 1102 88-89 1.8981856299796789 1102 90-91 1.6253355942746701 1102 92-93 1.6334324502960378 1102 94-95 1.6372843359000946 1102 96-97 1.7706046620434535 1102 98-99 1.5782729066766414 1102 100-101 1.432725267518368 1102 102-103 1.1782342668975723 1102 104-105 1.5832320497502188 1102 106-107 1.731575978735009 1102 108-109 1.7032100761266697 1102 110-111 1.5782472624558181 1102 112-113 1.5869425858302648 1102 114-115 1.839182987397848 1102 116-117 1.519468857632134 1102 118-119 1.0838849927961967 1102 120-121 1.479587617208658 1102 122-123 1.8117717271849578 1102 124-125 1.933672453085837 1103 1 1.308330834257088 1103 2 1.2121528344250265 1103 3 1.1424369898785152 1103 4 1.1341097495350816 1103 5 1.674192054911824 1103 6 1.7141008411475092 1103 7 1.988902929679746 1103 8 1.981029366569004 1103 9 2.71853175088782 1103 10-11 2.2959303542553258 1103 12-13 2.3014589794344325 1103 14-15 2.2008043218075386 1103 16-17 2.211843363591555 1103 18-19 2.495323259553693 1103 20-21 2.6974303490654705 1103 22-23 2.655626673156199 1103 24-25 2.890607918936471 1103 26-27 2.4050797752114725 1103 28-29 2.5271158212696285 1103 30-31 2.99057666214663 1103 32-33 2.8910968807426727 1103 34-35 2.891694766755073 1103 36-37 3.0206100379153433 1103 38-39 3.079014164768317 1103 40-41 3.355859315124107 1103 42-43 3.3115444774531397 1103 44-45 3.1199241453274347 1103 46-47 3.0500242220495295 1103 48-49 2.9733913584729805 1103 50-51 3.234876824560196 1103 52-53 3.0994672401585213 1103 54-55 3.365913482518504 1103 56-57 3.129925819335188 1103 58-59 3.2674889177049344 1103 60-61 3.262932420828186 1103 62-63 3.424878133021185 1103 64-65 3.3846369626009007 1103 66-67 3.620211630319538 1103 68-69 3.688206735980927 1103 70-71 3.4103674349299595 1103 72-73 3.4053912522838132 1103 74-75 3.3486198518543766 1103 76-77 3.225422997282866 1103 78-79 3.3882755191514775 1103 80-81 3.4418372210502213 1103 82-83 3.3551920595743496 1103 84-85 3.4664637633446596 1103 86-87 3.410679189460719 1103 88-89 3.2684583024783223 1103 90-91 3.3388277316848693 1103 92-93 3.2005610067438646 1103 94-95 3.20446796957102 1103 96-97 3.0877987592369287 1103 98-99 3.177959518427059 1103 100-101 3.301656079487717 1103 102-103 3.003448826601744 1103 104-105 3.0924169528419014 1103 106-107 3.0739833133193635 1103 108-109 3.0413472586564936 1103 110-111 3.0786567771323448 1103 112-113 3.040044905282759 1103 114-115 2.8514863076690276 1103 116-117 2.8607709682741103 1103 118-119 3.021731496369892 1103 120-121 3.0145828683791898 1103 122-123 3.1501184676252088 1103 124-125 2.877654202862768 1104 1 1.0890086294005705 1104 2 0.9844597491383311 1104 3 0.9997555251894319 1104 4 1.0347240960491497 1104 5 1.362673931606441 1104 6 1.5222693251103045 1104 7 1.8684882943290475 1104 8 1.722702122328947 1104 9 2.231232184697575 1104 10-11 1.7267107901511878 1104 12-13 1.7869152009342848 1104 14-15 1.8657642392067046 1104 16-17 1.8590318946803208 1104 18-19 1.8952557385830815 1104 20-21 2.1813203004248507 1104 22-23 2.104975403712011 1104 24-25 2.370629617746335 1104 26-27 2.207745632958055 1104 28-29 2.2761455332707747 1104 30-31 2.6200142795940096 1104 32-33 2.594395477116514 1104 34-35 2.4356940554824007 1104 36-37 2.4115237791818593 1104 38-39 2.657508042108738 1104 40-41 2.8436485886661558 1104 42-43 2.8307895353009087 1104 44-45 2.457276113782882 1104 46-47 2.485313749419376 1104 48-49 2.2173605484931933 1104 50-51 2.701494750739858 1104 52-53 2.4864475381274005 1104 54-55 2.7416383897673278 1104 56-57 2.690523920869637 1104 58-59 2.8310114762004552 1104 60-61 2.7567117254565083 1104 62-63 2.886935508646946 1104 64-65 2.9974956296137734 1104 66-67 2.9600398762778504 1104 68-69 3.0190771905906892 1104 70-71 2.8062548141675663 1104 72-73 2.9701035280623174 1104 74-75 3.0413930274427443 1104 76-77 2.9063737613461775 1104 78-79 2.9131716994174894 1104 80-81 2.6969721714469976 1104 82-83 2.718123989891815 1104 84-85 3.0027589204210727 1104 86-87 2.953092096008252 1104 88-89 2.8101129990140024 1104 90-91 2.8976520900553346 1104 92-93 2.592512667942831 1104 94-95 2.5818462251553704 1104 96-97 2.592018875381875 1104 98-99 2.6939386030157983 1104 100-101 2.6359222230749175 1104 102-103 2.6197753640076407 1104 104-105 2.558191116555566 1104 106-107 2.6762417705012496 1104 108-109 2.6209428873810374 1104 110-111 2.6340462211135183 1104 112-113 2.5790344375904617 1104 114-115 2.5051192354881664 1104 116-117 2.5054443015963486 1104 118-119 2.4814840600307377 1104 120-121 2.56689098149263 1104 122-123 2.776281928294104 1104 124-125 2.509126964503757 1105 1 0.8988098377505622 1105 2 1.0297334972915344 1105 3 0.942690371602211 1105 4 1.1937314084101764 1105 5 1.4293665759592606 1105 6 1.5124492766653113 1105 7 1.8912216157614026 1105 8 1.7362532285862997 1105 9 2.5093788878101435 1105 10-11 2.1763764486799175 1105 12-13 2.3147736854199685 1105 14-15 2.2429953777382465 1105 16-17 2.1981591095851414 1105 18-19 2.373804711988889 1105 20-21 2.5216481079669393 1105 22-23 2.534997903975743 1105 24-25 2.6189131132052204 1105 26-27 2.5861644164765956 1105 28-29 2.541947648911208 1105 30-31 2.874034932247703 1105 32-33 2.612453938481785 1105 34-35 2.7925771892608893 1105 36-37 2.5589634754305486 1105 38-39 2.67950949831215 1105 40-41 2.828855771854002 1105 42-43 2.813434825069322 1105 44-45 2.7212208735458354 1105 46-47 2.6142394573188135 1105 48-49 2.7308594990313715 1105 50-51 2.8896933972413663 1105 52-53 2.808808809812465 1105 54-55 2.9602546617450507 1105 56-57 2.871028657912131 1105 58-59 2.7312714009816297 1105 60-61 2.679807541775805 1105 62-63 2.559697410402311 1105 64-65 3.0544139776306594 1105 66-67 3.165426008911897 1105 68-69 3.057239392686604 1105 70-71 3.0009258253279363 1105 72-73 3.2000342488650304 1105 74-75 3.321757654619507 1105 76-77 3.019064632460715 1105 78-79 3.11147386645532 1105 80-81 3.056819221781282 1105 82-83 2.952714769434735 1105 84-85 3.243317246692648 1105 86-87 3.199978649539233 1105 88-89 3.084364088386046 1105 90-91 3.0594045570461255 1105 92-93 2.900482921264029 1105 94-95 2.8202591735246365 1105 96-97 2.7764245905926614 1105 98-99 2.921350261563177 1105 100-101 2.994339583194513 1105 102-103 2.6758943280293472 1105 104-105 2.3142894879152927 1105 106-107 2.5662229180483997 1105 108-109 2.621018326401874 1105 110-111 2.7732301192021964 1105 112-113 2.798908625750318 1105 114-115 2.7228651501253474 1105 116-117 2.630570843111009 1105 118-119 2.544146951862867 1105 120-121 2.6390751147173788 1105 122-123 2.747353675802227 1105 124-125 2.6449361038933823 1106 1 1.0296805489900471 1106 2 0.9438505451942305 1106 3 1.001254407530972 1106 4 0.881176407962986 1106 5 1.0954058301172793 1106 6 1.3933908889551994 1106 7 1.5378872737486375 1106 8 1.2684850546312667 1106 9 2.1241353658722915 1106 10-11 1.7091143715201014 1106 12-13 1.6133103201780763 1106 14-15 1.9188067545300669 1106 16-17 1.8596301696763362 1106 18-19 1.7973327158184702 1106 20-21 1.731563643035841 1106 22-23 1.703071914014032 1106 24-25 1.4363679902475077 1106 26-27 1.4998467899657193 1106 28-29 1.503290531025005 1106 30-31 1.9564699152893752 1106 32-33 1.7829322703329993 1106 34-35 1.7152385088452888 1106 36-37 1.8457905468418083 1106 38-39 1.9409976117278767 1106 40-41 2.117483231784483 1106 42-43 2.1858277053990065 1106 44-45 2.187763976982122 1106 46-47 2.036539146790748 1106 48-49 1.9666605210259895 1106 50-51 2.2997491009064888 1106 52-53 2.090934631836003 1106 54-55 2.4548694257534542 1106 56-57 2.2803377572973282 1106 58-59 2.251995187171861 1106 60-61 2.43861572292289 1106 62-63 2.476380219029256 1106 64-65 2.545097440586254 1106 66-67 2.549244674609735 1106 68-69 2.4719034329250356 1106 70-71 2.3883609080018005 1106 72-73 2.3055690721708046 1106 74-75 2.5222619441330565 1106 76-77 2.1589195610336986 1106 78-79 2.1495674252570858 1106 80-81 2.257370257330514 1106 82-83 2.0734188840590235 1106 84-85 2.3428646384839737 1106 86-87 2.377104128642653 1106 88-89 2.200721169246144 1106 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82-83 -10.183675587165524 2216 84-85 -10.186440159627354 2216 86-87 -10.618684796357437 2216 88-89 -10.323794947315314 2216 90-91 -9.693673266632345 2216 92-93 -9.235584901941685 2216 94-95 -9.06853817087722 2216 96-97 -9.167767832029677 2216 98-99 -9.139703424165553 2216 100-101 -9.48062733033011 2216 102-103 -9.070526287550887 2216 104-105 -9.195923531377034 2216 106-107 -9.365935405160284 2216 108-109 -9.288338293747822 2216 110-111 -9.032327646567516 2216 112-113 -9.102452214588029 2216 114-115 -9.022297882303404 2216 116-117 -8.789124670897689 2216 118-119 -9.02995428823089 2216 120-121 -8.972323037193396 2216 122-123 -9.052223271985596 2216 124-125 -8.82510025958328 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 159356.0 3 48944.0 4 23396.0 5 18724.0 6 19203.0 7 21848.0 8 23503.0 9 24770.0 10 25628.0 11 27000.0 12 28475.0 13 29275.0 14 28854.0 15 23055.0 16 17424.0 17 15282.0 18 13676.0 19 12990.0 20 12753.0 21 13802.0 22 17317.0 23 23519.0 24 32376.0 25 42106.0 26 50527.0 27 57331.0 28 64525.0 29 72888.0 30 80558.0 31 92622.0 32 107395.0 33 127413.0 34 155674.0 35 198241.0 36 280726.0 37 323819.0 38 93028.0 39 280.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.49390527743789 19.703027743788905 14.325091769963292 24.477975208809916 2 16.780018045976963 20.820930011423027 36.97818330244701 25.420868640153003 3 20.221957120844014 24.03804670757791 28.783172217117198 26.95682395446088 4 14.120740301368246 17.187207531128045 34.54641275619753 34.14563941130618 5 14.110641393545578 37.96690034435036 33.07507402515381 14.847384236950251 6 33.10897055269646 36.35918584961521 16.062229736968202 14.469613860720127 7 28.58204999975078 32.196581395387476 19.901625398956277 19.319743205905468 8 26.478852536412568 35.83664513975193 18.421892926263848 19.26260939757165 9 25.991206256023432 16.142985330334263 18.931214220137583 38.93459419350472 10-11 25.7651125414947 26.459775703876133 26.004514822657327 21.77059693197184 12-13 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26.376788967168995 23.928405188217596 62-63 23.587353768160444 26.000642364365927 26.39044168467218 24.02156218280145 64-65 23.504196450901816 25.929941164104868 26.286583405136778 24.279278979856535 66-67 23.461378128137017 26.13865194775684 26.117029706384088 24.282940217722054 68-69 23.43011041344469 25.980990891165906 26.342890207541398 24.246008487848002 70-71 23.308464559817725 25.952697425216165 26.485015411663532 24.253822603302577 72-73 23.51257996479804 26.145850473099085 26.007652022450806 24.33391753965207 74-75 23.309963055194636 26.252031092269828 26.099592764183182 24.338413088352358 76-77 23.499617254860926 26.093792594281222 26.2141699371808 24.192420213677053 78-79 23.505871542147396 26.185618422530542 26.086999850722627 24.22151018459943 80-81 23.32222383470162 26.291206720758776 26.142491828777942 24.24407761576166 82-83 23.427355395922437 26.287983224681312 26.14746916912345 24.137192210272808 84-85 23.364714357934684 26.114161323172652 25.980374143198375 24.54075017569429 86-87 23.396845657453664 25.931663800881864 26.226581500054603 24.444909041609865 88-89 23.280476558058922 26.05066128410074 26.20045767312539 24.468404484714956 90-91 23.273146958203245 26.24663818457607 26.147107271237722 24.333107585982958 92-93 23.403294967276008 26.05904249499169 26.13019379021725 24.40746874751505 94-95 23.17424309430349 25.89370951580708 26.294404144518317 24.637643245371112 96-97 23.241662976225253 25.984569283443964 26.215608563651134 24.558159176679652 98-99 23.16099759872408 25.833045094408803 26.188772758245122 24.817184548621995 100-101 23.041050044014817 25.946401581211486 26.19975667281213 24.812791701961565 102-103 23.04718269534609 25.610716707421943 26.466681365972434 24.875419231259528 104-105 23.018872233525723 25.67425154673421 26.458227795540424 24.84864842419964 106-107 22.738531131062466 25.865713685409887 26.46300173317134 24.93275345035631 108-109 22.97698836068385 25.668510150093237 26.230275011076266 25.124226478146642 110-111 22.726099701387056 25.78030616183231 26.447249233536578 25.04634490324405 112-113 22.771869397635886 25.780212411023946 26.388586981909455 25.059331209430706 114-115 22.713786221356607 25.763303110763424 26.365823685965967 25.157086981914002 116-117 22.492954582542147 25.79812423935028 26.515039843408406 25.193881334699164 118-119 22.552129993055217 25.717082609702143 26.354549352079747 25.376238045162893 120-121 22.475451074057034 25.72957481193828 26.506881339660467 25.28809277434421 122-123 22.496966712314777 25.681773449742927 26.44605398546181 25.375205852480487 124-125 22.38977497274338 25.708199784023332 26.220422854990627 25.681602388242663 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 483.0 1 378.0 2 430.5 3 605.0 4 456.0 5 367.0 6 702.0 7 1477.0 8 2309.0 9 2683.0 10 2232.5 11 1507.0 12 1327.5 13 1401.0 14 1527.5 15 1685.0 16 1896.0 17 2152.5 18 2396.0 19 2686.0 20 3067.0 21 3569.5 22 4207.5 23 5230.0 24 6558.0 25 8140.0 26 10131.5 27 12638.0 28 15805.5 29 18920.5 30 22084.5 31 25907.5 32 29211.0 33 31946.5 34 34487.0 35 37089.0 36 40837.0 37 45263.5 38 48841.5 39 51671.0 40 55127.0 41 59660.5 42 63975.5 43 67923.5 44 72210.0 45 76344.0 46 80120.0 47 83292.0 48 87794.5 49 91863.0 50 92914.0 51 93462.0 52 95329.5 53 97268.0 54 96787.5 55 93014.5 56 87919.5 57 82715.5 58 78574.5 59 72667.0 60 62980.5 61 51580.5 62 40778.5 63 31936.0 64 24622.5 65 19002.0 66 14900.0 67 11887.5 68 9119.0 69 6856.5 70 5110.5 71 3927.0 72 3134.0 73 2617.0 74 2096.5 75 1630.5 76 1178.5 77 727.5 78 432.5 79 260.5 80 168.0 81 115.0 82 78.0 83 54.0 84 31.5 85 16.0 86 11.5 87 8.5 88 5.0 89 4.0 90 3.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09496313379171974 2 4.1523014338312085E-4 3 0.0 4 0.17443818323524904 5 0.0 6 4.152301433831208E-5 7 0.03201424405483862 8 0.0 9 0.0 10-11 5.605606935672131E-4 12-13 6.020837079055251E-4 14-15 3.737071290448087E-4 16-17 0.0037370712904480873 18-19 0.0014740670090100788 20-21 0.0028443264821743776 22-23 0.0014325439946717667 24-25 0.0038824018406321795 26-27 0.0037370712904480873 28-29 0.0026367114104828174 30-31 7.058912437513054E-4 32-33 2.076150716915604E-5 34-35 0.001079598372796114 36-37 0.001079598372796114 38-39 4.98276172059745E-4 40-41 0.0014740670090100788 42-43 6.228452150746812E-4 44-45 9.550293297811778E-4 46-47 1.4533055018409226E-4 48-49 4.152301433831208E-5 50-51 0.03323917297781882 52-53 8.304602867662416E-5 54-55 0.1344099974131162 56-57 1.6609205735324833E-4 58-59 2.076150716915604E-5 60-61 0.0 62-63 5.190376792289011E-4 64-65 0.05269270519531803 66-67 0.04399363369144165 68-69 0.001515590023348391 70-71 0.002574426888975349 72-73 0.06722576021372725 74-75 0.004816669663244201 76-77 0.0017647281093782635 78-79 0.0014325439946717667 80-81 1.4533055018409226E-4 82-83 1.2456904301493625E-4 84-85 2.076150716915604E-5 86-87 1.6609205735324833E-4 88-89 0.0014117824875026109 90-91 2.491380860298725E-4 92-93 0.0022630042814380086 94-95 0.004567531577214329 96-97 0.0022630042814380086 98-99 0.0 100-101 9.550293297811778E-4 102-103 0.001079598372796114 104-105 1.2456904301493625E-4 106-107 4.152301433831208E-5 108-109 0.0 110-111 6.228452150746813E-5 112-113 2.076150716915604E-5 114-115 1.4533055018409226E-4 116-117 0.0 118-119 8.512217939353977E-4 120-121 0.002117673731253916 122-123 0.0010380753584578021 124-125 0.007162719973358833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2408303.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.722642640410825 #Duplication Level Percentage of deduplicated Percentage of total 1 78.93903788674098 45.565698743142 2 10.844049304229642 12.518943655260866 3 4.018389035962824 6.95856102939081 4 1.9867434862728313 4.587203371051624 5 1.105523366373723 3.190686510390719 6 0.6950779843492055 2.4073042858683755 7 0.4835563539947009 1.9538505432695277 8 0.32210112953023906 1.4874022715157336 9 0.2442527160888849 1.2689021020273583 >10 1.2489722071458549 12.810297634570327 >50 0.07690632461544274 3.032092464810983 >100 0.034141591369640116 3.1981184512523395 >500 8.813741124871785E-4 0.32721260409602954 >1k 2.2034352812167675E-4 0.23788415672220684 >5k 1.4689568541445116E-4 0.45584217663111176 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5537 0.22991293039123398 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5215 0.2165425197742975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3232 0.13420238234142465 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 2.076150716915604E-5 0.0 0.0 0.0 12-13 0.0 4.152301433831208E-5 0.0 2.076150716915604E-5 0.0 14-15 0.0 4.152301433831208E-5 0.0 8.304602867662416E-5 0.0 16-17 0.0 4.152301433831208E-5 0.0 1.0380753584578021E-4 0.0 18-19 0.0 4.152301433831208E-5 0.0 2.076150716915604E-4 0.0 20-21 0.0 4.152301433831208E-5 0.0 2.491380860298725E-4 0.0 22-23 0.0 4.152301433831208E-5 0.0 3.5294562187565266E-4 0.0 24-25 4.152301433831208E-5 4.152301433831208E-5 0.0 6.020837079055252E-4 0.0 26-27 4.152301433831208E-5 4.152301433831208E-5 0.0 7.474142580896175E-4 0.0 28-29 4.152301433831208E-5 4.152301433831208E-5 0.0 0.0012664519373185184 0.0 30-31 4.152301433831208E-5 4.152301433831208E-5 0.0 0.003238795118388342 0.0 32-33 4.152301433831208E-5 4.152301433831208E-5 0.0 0.006602159279791621 0.0 34-35 4.152301433831208E-5 4.152301433831208E-5 4.152301433831208E-5 0.011709490043404007 0.0 36-37 4.152301433831208E-5 4.152301433831208E-5 4.152301433831208E-5 0.02061617661897195 0.0 38-39 4.152301433831208E-5 4.152301433831208E-5 4.152301433831208E-5 0.03446410190079903 0.0 40-41 4.152301433831208E-5 4.152301433831208E-5 4.152301433831208E-5 0.05258889765947225 0.0 42-43 4.152301433831208E-5 4.152301433831208E-5 4.152301433831208E-5 0.07152339219774256 0.0 44-45 4.152301433831208E-5 8.304602867662416E-5 4.152301433831208E-5 0.09288698307480411 0.0 46-47 4.152301433831208E-5 8.304602867662416E-5 4.152301433831208E-5 0.12072816418864238 0.0 48-49 4.152301433831208E-5 8.304602867662416E-5 4.152301433831208E-5 0.15380124510910795 0.0 50-51 8.304602867662416E-5 8.304602867662416E-5 4.152301433831208E-5 0.1909020584203898 0.0 52-53 8.304602867662416E-5 8.304602867662416E-5 4.152301433831208E-5 0.22841810187505474 0.0 54-55 8.304602867662416E-5 8.304602867662416E-5 4.152301433831208E-5 0.2692559864767847 0.0 56-57 1.0380753584578021E-4 8.304602867662416E-5 4.152301433831208E-5 0.31933274176878906 4.152301433831208E-5 58-59 1.2456904301493625E-4 8.304602867662416E-5 4.152301433831208E-5 0.3745168278244058 4.152301433831208E-5 60-61 1.2456904301493625E-4 8.304602867662416E-5 4.152301433831208E-5 0.43626155014547585 4.152301433831208E-5 62-63 1.2456904301493625E-4 8.304602867662416E-5 4.152301433831208E-5 0.4988367327533122 4.152301433831208E-5 64-65 1.2456904301493625E-4 8.304602867662416E-5 4.152301433831208E-5 0.5666022921534375 4.152301433831208E-5 66-67 1.2456904301493625E-4 1.2456904301493625E-4 4.152301433831208E-5 0.6448524126739866 4.152301433831208E-5 68-69 1.2456904301493625E-4 1.2456904301493625E-4 4.152301433831208E-5 0.7393380318008158 4.152301433831208E-5 70-71 1.2456904301493625E-4 1.2456904301493625E-4 4.152301433831208E-5 0.8472355845589197 4.152301433831208E-5 72-73 1.2456904301493625E-4 1.2456904301493625E-4 4.152301433831208E-5 0.9614446354964471 6.228452150746813E-5 74-75 1.2456904301493625E-4 1.2456904301493625E-4 4.152301433831208E-5 1.0783526823659648 8.304602867662416E-5 76-77 1.2456904301493625E-4 1.2456904301493625E-4 4.152301433831208E-5 1.205724528848737 8.304602867662416E-5 78-79 1.2456904301493625E-4 1.2456904301493625E-4 4.152301433831208E-5 1.3509305099898143 1.0380753584578021E-4 80-81 1.2456904301493625E-4 1.2456904301493625E-4 4.152301433831208E-5 1.5192025255958241 1.2456904301493625E-4 82-83 1.2456904301493625E-4 1.453305501840923E-4 4.152301433831208E-5 1.699848399474651 1.2456904301493625E-4 84-85 1.2456904301493625E-4 1.6609205735324833E-4 4.152301433831208E-5 1.8817814867979652 1.2456904301493625E-4 86-87 1.2456904301493625E-4 1.8685356452240436E-4 4.152301433831208E-5 2.0747181729209325 1.2456904301493625E-4 88-89 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 2.2939389271200508 1.2456904301493625E-4 90-91 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 2.5474992141769537 1.2456904301493625E-4 92-93 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 2.825059803521401 1.2456904301493625E-4 94-95 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 3.1055062423623605 1.2456904301493625E-4 96-97 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 3.3914752421103156 1.2456904301493625E-4 98-99 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 3.697562142305183 1.2456904301493625E-4 100-101 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 4.049988726501607 1.453305501840923E-4 102-103 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 4.435862098747541 1.6609205735324833E-4 104-105 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 4.826988132307272 1.6609205735324833E-4 106-107 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 5.214543186633907 1.6609205735324833E-4 108-109 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 5.624665999253416 1.6609205735324833E-4 110-111 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 6.079010822143227 1.6609205735324833E-4 112-113 1.2456904301493625E-4 2.076150716915604E-4 4.152301433831208E-5 6.565930449781444 1.6609205735324833E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4100 0.0 67.347046 1 GTATCAA 7255 0.0 51.12984 1 TATCAAC 9845 0.0 37.762135 2 ATCAACG 9755 0.0 36.766888 3 TCAACGC 9710 0.0 36.69185 4 CAACGCA 9755 0.0 36.40775 5 AACGCAG 9850 0.0 35.331852 6 GTGGTAT 2065 0.0 28.88028 1 GTACATG 8230 0.0 28.840628 1 ACGCAGA 11950 0.0 28.674862 7 CGCAGAG 12280 0.0 28.332052 8 TGGTATC 2070 0.0 27.34267 2 GAGTACT 7480 0.0 26.795807 12-13 GCAGAGT 12790 0.0 25.946823 9 TACATGG 8905 0.0 25.490519 2 GTACTTT 8210 0.0 24.666784 14-15 AGTACTT 7855 0.0 24.418674 12-13 AGAGTAC 11675 0.0 24.172295 10-11 ACATGGG 8865 0.0 23.858177 3 CAGAGTA 12530 0.0 23.401 10-11 >>END_MODULE