FastQCFastQC Report
Thu 26 May 2016
SRR1364508_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364508_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2695560
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43000.15952158364124708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.11159091246345844No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46350.059.4571081
GTATCAA86900.055.3431781
ATCAACG104750.043.4520843
TCAACGC107350.042.2867774
CAACGCA108800.041.777975
AACGCAG110200.040.253956
TATCAAC118600.040.132892
GTACATG149350.037.462361
TACATGG151350.036.2053152
ACGCAGA130750.033.381377
CGCAGAG129150.032.966878
ACATGGG156300.032.623093
CGCATAG3800.029.7739248
GCAGAGT138100.029.493659
CATGGGA93500.027.7159214
ACGCATA4200.026.9368117
GAGTACT61300.026.20075612-13
CATGGGG67450.025.8783954
GAGTACA112600.025.3203471
AACGCAT5250.024.951536
Produced by FastQC (version 0.11.5)