##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364508_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2695560 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.969232738280727 31.0 26.0 31.0 10.0 34.0 2 27.12838074463191 31.0 27.0 31.0 10.0 34.0 3 27.071325809850272 31.0 27.0 31.0 10.0 34.0 4 29.885910534360207 35.0 28.0 37.0 10.0 37.0 5 30.54375380254938 35.0 30.0 37.0 12.0 37.0 6 30.69365586371663 35.0 31.0 37.0 11.0 37.0 7 30.476957663713662 35.0 30.0 37.0 10.0 37.0 8 30.111908100728606 35.0 30.0 37.0 10.0 37.0 9 30.72607324637552 35.0 29.0 39.0 8.0 39.0 10-11 30.84956001721349 36.0 29.5 39.0 8.0 39.0 12-13 30.729019572927335 35.0 29.5 39.0 8.0 39.0 14-15 31.461738748163647 37.0 29.0 40.0 8.0 41.0 16-17 31.75309842852691 37.0 30.0 40.0 8.0 41.0 18-19 31.723482133582632 37.0 30.0 40.0 7.0 41.0 20-21 31.5170576800368 37.0 29.5 40.0 2.0 41.0 22-23 31.587432110581844 37.0 29.5 40.0 2.0 41.0 24-25 31.624006328926086 37.0 30.0 40.0 2.0 41.0 26-27 31.758503427859146 37.0 30.0 40.0 2.0 41.0 28-29 31.71546561753402 37.0 30.0 40.0 2.0 41.0 30-31 31.357592299930257 37.0 30.0 40.0 2.0 41.0 32-33 31.373682092032823 37.0 30.0 40.0 2.0 41.0 34-35 31.067566850672957 36.0 29.5 40.0 2.0 41.0 36-37 30.82571933104809 36.0 29.0 40.0 2.0 41.0 38-39 30.683860125539777 36.0 28.5 40.0 2.0 41.0 40-41 30.739814546884503 36.0 29.0 40.0 2.0 41.0 42-43 30.58901953582929 36.0 28.0 40.0 2.0 41.0 44-45 30.23920317114069 36.0 27.5 40.0 2.0 41.0 46-47 29.954355310213835 35.0 27.0 40.0 2.0 41.0 48-49 29.688988373473414 35.0 26.5 40.0 2.0 41.0 50-51 29.673887429699207 35.0 27.0 40.0 2.0 41.0 52-53 29.483241144697203 35.0 26.5 40.0 2.0 41.0 54-55 29.367699847156064 35.0 26.5 39.5 2.0 41.0 56-57 28.954809761236998 35.0 26.0 39.0 2.0 41.0 58-59 28.727384847675438 34.0 25.0 39.0 2.0 41.0 60-61 28.31771301696123 34.0 23.5 39.0 2.0 40.0 62-63 28.26848706762231 34.0 24.5 38.5 2.0 40.0 64-65 28.090403849292912 34.0 24.0 38.0 2.0 40.0 66-67 27.857907262312843 34.0 24.0 38.0 2.0 40.0 68-69 27.678810896437106 33.5 23.5 37.0 2.0 40.0 70-71 27.536581452462567 34.0 23.5 37.0 2.0 40.0 72-73 27.49193228865245 34.0 24.5 37.0 2.0 39.0 74-75 27.20145146092092 33.5 24.0 36.0 2.0 39.0 76-77 26.92301562569559 33.0 23.5 36.0 2.0 39.0 78-79 26.606524803751356 33.0 22.5 35.0 2.0 37.5 80-81 26.580963510365194 33.0 24.0 35.0 2.0 37.0 82-83 26.37106668002196 33.0 24.0 35.0 2.0 37.0 84-85 26.18064131386428 33.0 23.0 35.0 2.0 36.0 86-87 25.96695269257594 33.0 23.0 35.0 2.0 36.0 88-89 25.75343138346021 33.0 20.5 35.0 2.0 36.0 90-91 25.590212608882755 33.0 20.0 35.0 2.0 35.0 92-93 25.48586787161109 33.0 20.0 35.0 2.0 35.0 94-95 25.402636557895207 33.0 20.0 35.0 2.0 35.0 96-97 25.27726019825194 33.0 19.0 35.0 2.0 35.0 98-99 25.17557631809346 33.0 18.5 35.0 2.0 35.0 100-101 25.125190312959088 33.0 18.0 35.0 2.0 35.0 102-103 23.97627413227678 30.5 15.0 34.0 2.0 35.0 104-105 24.75017157844752 32.0 15.5 35.0 2.0 35.0 106-107 24.848365274748105 32.0 15.0 35.0 2.0 35.0 108-109 24.777775119084716 32.5 12.5 35.0 2.0 35.0 110-111 24.663710880113964 32.0 7.5 35.0 2.0 35.0 112-113 24.639212260161152 32.5 7.0 35.0 2.0 35.0 114-115 24.587718878451973 33.0 2.0 35.0 2.0 35.0 116-117 24.40515403107332 32.5 2.0 35.0 2.0 35.0 118-119 24.250339818071197 32.0 2.0 35.0 2.0 35.0 120-121 24.108179747436523 32.0 2.0 35.0 2.0 35.0 122-123 23.832288652450696 32.0 2.0 35.0 2.0 35.0 124-125 22.82663862054638 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6468727618142012 1101 2 1.3187232633144887 1101 3 1.6571079138926805 1101 4 2.027395241027346 1101 5 1.453164786461251 1101 6 1.3678853927525658 1101 7 1.3744167100679618 1101 8 1.7318154088864262 1101 9 2.1814607707054137 1101 10-11 2.123759522300169 1101 12-13 1.9743093839412644 1101 14-15 2.338702212945421 1101 16-17 1.9917101137652367 1101 18-19 2.014797841585512 1101 20-21 2.346310564059749 1101 22-23 2.1344353257047395 1101 24-25 2.059754395234652 1101 26-27 1.749259503925618 1101 28-29 1.684088008441467 1101 30-31 1.8394056365247948 1101 32-33 1.9953698853567445 1101 34-35 2.322688380873913 1101 36-37 2.2857770223324394 1101 38-39 2.3309335598432988 1101 40-41 2.1560637463304424 1101 42-43 2.0903556440122983 1101 44-45 2.267358761206978 1101 46-47 2.296107599607655 1101 48-49 2.274784462767844 1101 50-51 2.2014937257493052 1101 52-53 2.1261056099835436 1101 54-55 2.1152020005861942 1101 56-57 2.3088037558633268 1101 58-59 2.319811561037767 1101 60-61 2.5804070399780628 1101 62-63 2.268024034118387 1101 64-65 2.1951624809469763 1101 66-67 2.3587622546477114 1101 68-69 2.152286378158827 1101 70-71 2.0990514874402315 1101 72-73 1.9142115657504988 1101 74-75 1.9823882463192817 1101 76-77 2.1857381472886885 1101 78-79 2.060322060616919 1101 80-81 1.9311691435484875 1101 82-83 1.993507325391974 1101 84-85 1.8186323688177524 1101 86-87 1.8133965062565238 1101 88-89 1.9617557463280697 1101 90-91 1.985297359858329 1101 92-93 1.979171173162804 1101 94-95 1.8205816316893433 1101 96-97 1.8485512990994906 1101 98-99 1.8236442208364068 1101 100-101 1.7521903049121121 1101 102-103 1.7924665281755736 1101 104-105 1.756124085201975 1101 106-107 1.885091608472159 1101 108-109 1.952102996934233 1101 110-111 1.9962750285786584 1101 112-113 1.8718564229170198 1101 114-115 1.9052884185120824 1101 116-117 2.0801309108642236 1101 118-119 1.9631235429747917 1101 120-121 1.9757723028173757 1101 122-123 1.9323656307238188 1101 124-125 1.955550032891889 1102 1 0.9121846836831509 1102 2 0.7267665451627856 1102 3 0.8064974075036595 1102 4 1.190384528124536 1102 5 1.0775563236982677 1102 6 1.0134557795356827 1102 7 0.8539079547437751 1102 8 1.107455386514097 1102 9 1.5345164218691991 1102 10-11 1.2282374296271072 1102 12-13 1.555995267863306 1102 14-15 1.8292283390909319 1102 16-17 1.3864057748244747 1102 18-19 1.5147786422566867 1102 20-21 1.6032321641113754 1102 22-23 1.5633301542552722 1102 24-25 1.3395568626441978 1102 26-27 1.338846960364112 1102 28-29 1.1290652058772501 1102 30-31 0.9617304762614864 1102 32-33 0.8944228260270606 1102 34-35 1.392662566650273 1102 36-37 1.5804492354718747 1102 38-39 1.5477365758051 1102 40-41 1.1535856884205344 1102 42-43 1.1264448429108853 1102 44-45 1.5626650362145078 1102 46-47 1.5206372967207606 1102 48-49 1.6262116128775546 1102 50-51 1.4477816941699047 1102 52-53 1.5257488359010232 1102 54-55 1.2536718087004637 1102 56-57 1.3390091402801403 1102 58-59 1.4444210133459912 1102 60-61 1.9240717992604246 1102 62-63 1.590564799079882 1102 64-65 1.716623135767783 1102 66-67 1.6333314260111322 1102 68-69 1.4285263751471682 1102 70-71 1.7204101222980377 1102 72-73 1.6197349491347452 1102 74-75 1.5769839848211973 1102 76-77 1.4632153985041079 1102 78-79 1.4598763888017494 1102 80-81 1.074607544357331 1102 82-83 1.016685056321716 1102 84-85 1.0522136083307245 1102 86-87 1.1321966826537206 1102 88-89 0.8866173713334469 1102 90-91 0.7976476373612336 1102 92-93 0.7975404671437865 1102 94-95 1.0239852370759088 1102 96-97 1.1727864451649737 1102 98-99 1.3128137557501454 1102 100-101 1.1856751713235028 1102 102-103 0.976749161656624 1102 104-105 0.9815189352643579 1102 106-107 1.2392068259569946 1102 108-109 1.344159906541428 1102 110-111 1.20223101532736 1102 112-113 1.0744143973609361 1102 114-115 1.08105703702088 1102 116-117 1.3309281939171882 1102 118-119 1.3913455345421468 1102 120-121 1.4084170238086386 1102 122-123 1.5376357658182584 1102 124-125 1.467827008755588 1103 1 1.3005018510605773 1103 2 1.0785582385815609 1103 3 1.1787540044276597 1103 4 1.74548727245271 1103 5 1.1738912901815794 1103 6 1.1915648831295869 1103 7 1.235338063520178 1103 8 1.5036697548148439 1103 9 2.133374039192752 1103 10-11 1.7015173474654048 1103 12-13 1.8218953256630037 1103 14-15 2.067370635857671 1103 16-17 1.812613125362688 1103 18-19 1.5460495043032267 1103 20-21 1.965807851751478 1103 22-23 1.7584718847104348 1103 24-25 1.794558246504554 1103 26-27 1.6220862799159796 1103 28-29 1.5413497202093502 1103 30-31 1.7196246476234336 1103 32-33 1.6855458216746584 1103 34-35 1.611942651980037 1103 36-37 1.9532982739431972 1103 38-39 1.9138864425801323 1103 40-41 1.8721587498637184 1103 42-43 1.8458864146819494 1103 44-45 1.8337976875070154 1103 46-47 1.986182473864627 1103 48-49 1.9316786619614277 1103 50-51 1.8763660602049725 1103 52-53 1.8177792392806325 1103 54-55 1.8049563860041573 1103 56-57 1.9411898077401197 1103 58-59 2.1433017094353275 1103 60-61 2.0534169747163915 1103 62-63 1.8972456743894135 1103 64-65 1.734836588275403 1103 66-67 1.7959453510437697 1103 68-69 1.6620227854415326 1103 70-71 1.7498240796171451 1103 72-73 1.682450332013957 1103 74-75 1.7338924342895154 1103 76-77 1.7560387354750766 1103 78-79 1.9130482421856954 1103 80-81 1.4768392707463924 1103 82-83 1.5569828210896937 1103 84-85 1.5413012905453165 1103 86-87 1.5028788814487761 1103 88-89 1.6132216917909297 1103 90-91 1.6212782593886104 1103 92-93 1.5271153266316482 1103 94-95 1.396382438107434 1103 96-97 1.5472274640410895 1103 98-99 1.504079541117406 1103 100-101 1.2836005493316165 1103 102-103 1.3194168440919398 1103 104-105 1.4692282752506074 1103 106-107 1.3997048187643841 1103 108-109 1.5158701437106359 1103 110-111 1.644109962277792 1103 112-113 1.5246890703792957 1103 114-115 1.4153212571868998 1103 116-117 1.5407387900677847 1103 118-119 1.7192324198089786 1103 120-121 1.7271404596947875 1103 122-123 1.7907262430196198 1103 124-125 1.8449801883559935 1104 1 1.3669510469195387 1104 2 1.1621489539448682 1104 3 1.1926173818818597 1104 4 1.555941023468531 1104 5 1.1554045192262308 1104 6 1.0288250189514336 1104 7 1.0084067912221535 1104 8 1.2196832669075732 1104 9 1.836554835792711 1104 10-11 1.7003612929371101 1104 12-13 1.2119518136486178 1104 14-15 1.5952219071826548 1104 16-17 1.2541288571048526 1104 18-19 0.756231463193842 1104 20-21 -3.910300736046814 1104 22-23 -0.49937625721189605 1104 24-25 0.8090767782168662 1104 26-27 0.9980734435928049 1104 28-29 0.5625833769142865 1104 30-31 1.2530769415498 1104 32-33 1.1254778860125398 1104 34-35 1.3164792653726423 1104 36-37 1.440823440071597 1104 38-39 1.3761571437436224 1104 40-41 1.4857163817923684 1104 42-43 1.3969997336917714 1104 44-45 1.5626287628464581 1104 46-47 1.579003226298365 1104 48-49 1.5097484967050505 1104 50-51 1.389404722552321 1104 52-53 1.5192031999499385 1104 54-55 1.444773994192225 1104 56-57 1.734047935714134 1104 58-59 1.6560721151815514 1104 60-61 1.7512120239931441 1104 62-63 1.4113898837781953 1104 64-65 1.5552907701510073 1104 66-67 1.546140011021187 1104 68-69 1.5004072282285073 1104 70-71 1.804084994285052 1104 72-73 1.5735291327641932 1104 74-75 1.5738960543363305 1104 76-77 1.6156749248934084 1104 78-79 1.6261152608792386 1104 80-81 1.4289807469586044 1104 82-83 1.37928361853076 1104 84-85 1.3653451116655262 1104 86-87 1.2912034986329495 1104 88-89 1.425960906587104 1104 90-91 1.4147114511772898 1104 92-93 1.0931557210765668 1104 94-95 1.187007568241519 1104 96-97 1.3094798588175003 1104 98-99 1.1993719444203066 1104 100-101 0.6846399934111673 1104 102-103 1.1401922638916169 1104 104-105 1.2806676953339533 1104 106-107 1.44258402588342 1104 108-109 1.479006739044241 1104 110-111 1.438750231464141 1104 112-113 1.3655128701667927 1104 114-115 1.3248121499654815 1104 116-117 1.4363409485722336 1104 118-119 1.3778689114476173 1104 120-121 1.4239460260733772 1104 122-123 1.1694911123206708 1104 124-125 1.3057856054798087 1105 1 1.35766887321725 1105 2 1.1349870573239471 1105 3 1.1562986653377685 1105 4 1.6762444265239296 1105 5 1.2886679393776994 1105 6 1.151291856231282 1105 7 1.0536363632471506 1105 8 1.3896083673730253 1105 9 1.9283985006055673 1105 10-11 1.6574274151010115 1105 12-13 1.719183267283352 1105 14-15 1.957400978053144 1105 16-17 1.5898269030453136 1105 18-19 1.3812816828887193 1105 20-21 1.6196283517633816 1105 22-23 1.532156029856079 1105 24-25 1.5252582050087007 1105 26-27 1.5142023573150034 1105 28-29 0.9636229532654248 1105 30-31 1.3113873758836974 1105 32-33 1.2625572211423517 1105 34-35 1.2933452384671895 1105 36-37 1.4744639640034904 1105 38-39 1.331891258214295 1105 40-41 1.4901007930987014 1105 42-43 1.6750410092251187 1105 44-45 1.8066854821738758 1105 46-47 2.058455865503607 1105 48-49 1.7094855925629027 1105 50-51 1.7539247557020126 1105 52-53 1.7769818580129702 1105 54-55 1.7305192628037425 1105 56-57 2.105497791596381 1105 58-59 1.8810871784839094 1105 60-61 2.2324249065045976 1105 62-63 2.116776004691168 1105 64-65 1.7996764063279542 1105 66-67 1.9133057386203873 1105 68-69 1.4399433671236217 1105 70-71 1.6700203523903099 1105 72-73 1.5489934890295345 1105 74-75 1.7563628916161775 1105 76-77 1.6820485781872705 1105 78-79 1.6496579775901168 1105 80-81 1.553851093101823 1105 82-83 1.5459140516137317 1105 84-85 1.5556758527904293 1105 86-87 1.584122990754686 1105 88-89 1.726797128356445 1105 90-91 1.717907711934 1105 92-93 1.6026801064260745 1105 94-95 1.491995189230071 1105 96-97 1.6173571319949183 1105 98-99 1.4319928808469164 1105 100-101 1.528371991722409 1105 102-103 1.627567027387343 1105 104-105 1.6931879317600398 1105 106-107 1.7699681192446235 1105 108-109 1.8242489506914552 1105 110-111 1.828384855168256 1105 112-113 1.8974922610673062 1105 114-115 1.7065758593948992 1105 116-117 1.928605030675051 1105 118-119 2.0246310311513547 1105 120-121 2.0481718036056016 1105 122-123 2.08891253035598 1105 124-125 1.6981295126606746 1106 1 -0.339803797594314 1106 2 -0.2468760285734639 1106 3 -0.2341177447556575 1106 4 -0.5239158076032915 1106 5 -0.9663257373333956 1106 6 -0.5255757652189033 1106 7 -0.5433782498404014 1106 8 -0.13575722466558915 1106 9 -0.13236069133769846 1106 10-11 -2.5798403943925265 1106 12-13 -0.6531429746966602 1106 14-15 -0.35411786932053246 1106 16-17 -0.46001213449051903 1106 18-19 -3.219836777804261 1106 20-21 -5.661132032003977 1106 22-23 -6.5758893124291795 1106 24-25 -6.393760261643404 1106 26-27 -5.255938741414379 1106 28-29 -1.9186241648377198 1106 30-31 -3.7090900158755 1106 32-33 -4.236236123182248 1106 34-35 -3.6871148056303085 1106 36-37 -6.093451734306111 1106 38-39 -6.420742739229905 1106 40-41 -2.7012410021856468 1106 42-43 -1.3912885036498963 1106 44-45 -0.6419712658678947 1106 46-47 -0.4539711518241951 1106 48-49 -0.3540116165823619 1106 50-51 -0.36514469453602416 1106 52-53 -0.6045506953377071 1106 54-55 -0.5820224344861806 1106 56-57 -0.41170923486873434 1106 58-59 -0.22362304273188727 1106 60-61 0.008934583454056622 1106 62-63 -0.17754349686151372 1106 64-65 -0.2775116833766731 1106 66-67 -0.6088925146952029 1106 68-69 -0.5985679121671126 1106 70-71 -0.30210036614719016 1106 72-73 -0.3105379916794675 1106 74-75 -0.33674757061292837 1106 76-77 -0.35519658664862064 1106 78-79 -0.3633641572316719 1106 80-81 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-4.87675654275742 2216 76-77 -4.293451041194626 2216 78-79 -4.5877925055225255 2216 80-81 -4.6455170675628565 2216 82-83 -4.864682223510055 2216 84-85 -4.628446646455643 2216 86-87 -4.916873285431905 2216 88-89 -5.039211403334495 2216 90-91 -5.091062254441415 2216 92-93 -4.863171182826417 2216 94-95 -7.980886787273903 2216 96-97 -10.107648268398053 2216 98-99 -6.860478081246711 2216 100-101 -5.786399125939731 2216 102-103 -6.751562665740138 2216 104-105 -9.659140587496047 2216 106-107 -6.171292503853117 2216 108-109 -8.681977961466135 2216 110-111 -6.061931130924901 2216 112-113 -5.420516594780061 2216 114-115 -5.86001046784633 2216 116-117 -5.40057428229931 2216 118-119 -5.696311148109807 2216 120-121 -6.486502226548918 2216 122-123 -9.373978652721991 2216 124-125 -7.80294485334116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 211188.0 3 32650.0 4 19106.0 5 16253.0 6 16868.0 7 18634.0 8 20315.0 9 21999.0 10 23276.0 11 24406.0 12 25530.0 13 26999.0 14 29064.0 15 24268.0 16 19117.0 17 17004.0 18 15375.0 19 14264.0 20 14447.0 21 15733.0 22 19692.0 23 26657.0 24 36096.0 25 45496.0 26 55206.0 27 62463.0 28 71407.0 29 82797.0 30 92655.0 31 105018.0 32 125513.0 33 154003.0 34 192734.0 35 246330.0 36 322742.0 37 348506.0 38 101452.0 39 297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.65703989887512 18.92359486473359 13.704330040239027 22.715035196152265 2 15.144266362563888 20.6211593633881 40.54231579509875 23.692258478949256 3 18.729631478447082 26.49703182323436 29.081748728388167 25.691587969930392 4 11.10928003414711 16.918186120312892 38.392933032690294 33.579600812849705 5 13.36547262802577 39.24129191650218 33.2288707274324 14.164364728039654 6 30.988374508802963 37.05814526373274 17.980594007099356 13.972886220364941 7 28.19713949913039 33.790594003573396 20.448292543940354 17.56397395335585 8 26.09496511559748 35.22081830505507 20.101065712375636 18.58315086697182 9 26.557749392571505 16.845052217146456 20.461760665197733 36.1354377250843 10-11 24.48593908722716 27.63601861041101 27.06279092687709 20.815251375484735 12-13 25.27505326693347 25.205675474430944 27.72013814713806 21.799133111497532 14-15 22.675490034939024 26.223264708097567 25.66246683538217 25.438778421581237 16-17 22.22764992074282 29.820866159262405 25.84014094123095 22.111342978763826 18-19 22.038498343461058 28.04400393754984 28.477089893906555 21.44040782508255 20-21 23.168854596249492 27.161401203635236 28.257659889555015 21.412084310560253 22-23 22.747865326626453 27.151861700844815 27.978441218146866 22.121831754381862 24-25 22.397053397158583 27.360206947869553 28.13579766630806 22.1069419886638 26-27 22.253448597614206 27.661943383675197 27.94949192523033 22.135116093480267 28-29 21.985682464729265 27.557248623228304 28.239364614852665 22.21770429718977 30-31 22.10035848676515 27.273430465698905 28.376844789662165 22.249366257873778 32-33 22.055261606018746 27.382785986166326 28.00711365505303 22.554838752761903 34-35 22.588989006520098 27.332496738467384 27.984024055936587 22.094490199075935 36-37 22.559740928490445 27.098556393833807 27.902330278333444 22.439372399342304 38-39 22.282649930518545 27.39750905596304 27.999570366970794 22.32027064654762 40-41 22.415416053800026 27.211538586430013 28.10217522119329 22.27087013857667 42-43 22.3793352166857 27.21867145741642 27.853356752797758 22.54863657310012 44-45 22.39170984840818 27.43872308936462 27.65646862642602 22.513098435801187 46-47 22.32649283874806 27.633244770294755 27.341919799097997 22.698342591859188 48-49 22.60320413133744 27.584075473560194 27.226675754085395 22.58604464101697 50-51 22.43755668506746 27.745069175956573 27.0187397192372 22.798634419738768 52-53 22.832960577966226 27.62696067591654 26.964961242841095 22.57511750327614 54-55 22.454176494006017 27.528812330560715 27.1163516167295 22.90065955870377 56-57 22.48076755054344 27.96448514949379 26.96549780837912 22.58924949158365 58-59 22.586977271399142 27.47781449981402 27.050494912663293 22.884713316123552 60-61 22.64426289218063 27.51910260213985 27.30410845338735 22.532526052292173 62-63 22.468761953452447 27.55214441458645 27.245449664997253 22.733643966963857 64-65 22.335570873174788 27.325718797152597 27.125356769068155 23.213353560604457 66-67 22.395994685601224 27.840990250133657 26.886205306310686 22.87680975795444 68-69 22.413092342672826 27.51423611027376 27.074886290130046 22.99778525692337 70-71 22.05932656135222 27.58666552893427 27.445871144385663 22.908136765327853 72-73 22.455402547069138 27.531436773368647 27.134371929522565 22.87878875003965 74-75 22.23256450670178 27.699368710500348 26.96733777540429 23.100729007393582 76-77 22.422457852477503 27.547128369886448 27.20950668463111 22.82090709300494 78-79 22.49064562619017 27.62627476998111 27.035272037509518 22.847807566319208 80-81 22.26484924909283 27.929550769011595 27.011233278908747 22.794366702986824 82-83 22.434151007900972 27.906379264001995 26.807017882314444 22.85245184578259 84-85 22.38123371690863 27.56446226032727 26.840897096891176 23.213406925872924 86-87 22.358346805122338 27.453103798163387 27.16116711603546 23.02738228067881 88-89 22.43962007738395 27.387807557419315 27.037747028167715 23.134825337029024 90-91 22.200599055097893 27.668817915186604 27.236453506264258 22.89412952345124 92-93 22.414912804162412 27.442040872127176 27.1052703337154 23.037775989995012 94-95 22.279407331833887 27.272363020845408 27.164606858812345 23.28362278850836 96-97 22.462297734237634 27.289575252333105 27.05227185586912 23.195855157560143 98-99 22.02535665447088 27.22610858382187 27.10290168750042 23.645633074206835 100-101 21.973978978469283 27.329140008558788 27.293432009837225 23.4034490031347 102-103 22.447827649926527 27.004526370812197 27.230084395057457 23.317561584203816 104-105 22.19542191470812 27.06857853048656 27.351815095809606 23.384184458995716 106-107 22.00518227268756 27.33022807527484 27.382759381206444 23.281830270831158 108-109 22.0569091364391 27.387948544346646 26.966861743074173 23.588280576140082 110-111 21.775886637435153 27.3150217789473 27.430288262666608 23.478803320950938 112-113 22.01708995832781 27.26574579127546 27.375672083526176 23.34149216687056 114-115 21.642558495857898 27.598117596429596 27.162185725044424 23.597138182668086 116-117 21.51376374835183 27.505385072571414 27.397052920256403 23.58379825882035 118-119 21.79783142645305 27.531364330038844 27.00852192678908 23.66228231671903 120-121 21.52368924741196 27.53555251413623 27.28000403653809 23.66075420191372 122-123 21.679919953462004 27.438644343467022 27.29621819433768 23.585217508733294 124-125 21.69172773944032 27.533964084068007 26.98804429348265 23.78626388300902 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1720.0 1 1720.0 2 2062.5 3 2173.0 4 1678.0 5 1234.0 6 1062.5 7 1250.5 8 1648.0 9 1820.5 10 1649.5 11 1433.5 12 1448.0 13 1647.0 14 1874.5 15 2083.0 16 2333.0 17 2639.0 18 2986.0 19 3344.0 20 3926.5 21 4837.0 22 5801.5 23 6890.5 24 8327.5 25 10794.5 26 14674.0 27 19304.5 28 23129.5 29 27098.5 30 32709.0 31 38292.0 32 43808.5 33 49728.5 34 56116.5 35 61377.5 36 66499.0 37 72710.0 38 76150.0 39 77168.5 40 78131.0 41 81817.0 42 86670.5 43 90091.0 44 92704.0 45 93930.0 46 93943.5 47 94876.5 48 100032.5 49 103011.5 50 100468.5 51 96548.5 52 93220.5 53 92420.5 54 89574.5 55 82193.5 56 74156.5 57 67037.5 58 60815.5 59 54314.5 60 47102.0 61 39001.0 62 31370.0 63 24660.5 64 19121.0 65 15445.5 66 12198.0 67 9774.0 68 7531.5 69 5471.0 70 4302.0 71 3580.0 72 3093.0 73 2695.0 74 2171.5 75 1701.5 76 1240.5 77 827.5 78 591.5 79 405.0 80 295.5 81 206.5 82 134.5 83 99.5 84 72.0 85 48.5 86 37.0 87 34.0 88 26.5 89 21.0 90 14.5 91 9.0 92 5.5 93 2.5 94 4.0 95 5.0 96 2.0 97 0.5 98 1.5 99 2.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09838400925967145 2 0.03179302260012762 3 0.042477258899820444 4 0.04952588701420113 5 0.05394055409636587 6 0.0038581964415557433 7 0.003264627758239475 8 0.003190431672824942 9 0.006714745730015285 10-11 0.06275133923934173 12-13 0.006417961388357151 14-15 0.010869726513229162 16-17 0.019884550891094983 18-19 0.017918354627609847 20-21 0.13472154209143927 22-23 0.01546988380893024 24-25 0.014412589591773137 26-27 0.007011530071673419 28-29 0.004600157295701079 30-31 0.002337176690557806 32-33 0.006696196708661652 34-35 0.018845805695291516 36-37 0.003264627758239475 38-39 0.008773687100268589 40-41 0.033926160055795455 42-43 0.013577883630859634 44-45 0.11008844173381412 46-47 0.004321921975396578 48-49 0.009589844039828458 50-51 0.01096247161999733 52-53 0.011722981495496299 54-55 0.010127765659083828 56-57 0.006696196708661652 58-59 0.014375491549065872 60-61 0.030995414681921383 62-63 0.007753490925818753 64-65 0.007011530071673419 66-67 0.00894062829245129 68-69 0.007920432118001454 70-71 0.005193725979017347 72-73 0.0011314903025716363 74-75 0.0016879609431806376 76-77 0.008736589057561323 78-79 0.020923296086898455 80-81 0.0032275297155322084 82-83 0.0048783926160055795 84-85 0.0032460787368858417 86-87 0.004173529804567511 88-89 0.006269569217528083 90-91 0.005509059342029114 92-93 0.0038767454629093766 94-95 0.004544510231640178 96-97 0.0024299217973259733 98-99 0.003487216014483076 100-101 0.0031718826514713083 102-103 8.532549822671356E-4 104-105 0.004080784697799344 106-107 9.089020463280358E-4 108-109 0.0012984314947543367 110-111 0.002485568861386873 112-113 0.004359020018103845 114-115 0.0025968629895086735 116-117 0.005416314235260947 118-119 0.0037654513347875765 120-121 0.006714745730015285 122-123 0.0047485494665301465 124-125 0.05841086824259152 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2695560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.84956001402455 #Duplication Level Percentage of deduplicated Percentage of total 1 78.29917285405791 42.9467518050713 2 11.043221728106708 12.114317058479376 3 4.203999057993494 6.917624958909505 4 2.072365346911788 4.546733098656917 5 1.1579739146006356 3.175717986178124 6 0.7235479913453475 2.381177338459413 7 0.47746809510991484 1.8332240456259272 8 0.33659866665785254 1.476983101399242 9 0.24900805256707995 1.2292183910928087 >10 1.2823395347112985 12.624849739206113 >50 0.0941809560467472 3.5988264598059954 >100 0.05690095111976863 5.586765979528523 >500 0.00264989952327981 0.941055279509447 >1k 5.729512482731722E-4 0.6267547580772963 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4300 0.15952158364124708 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3008 0.11159091246345844 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.709804270726676E-5 0.0 5 0.0 0.0 0.0 3.709804270726676E-5 0.0 6 0.0 0.0 0.0 3.709804270726676E-5 0.0 7 0.0 0.0 0.0 3.709804270726676E-5 0.0 8 0.0 0.0 0.0 3.709804270726676E-5 0.0 9 0.0 0.0 0.0 3.709804270726676E-5 0.0 10-11 0.0 0.0 0.0 5.5647064060900144E-5 0.0 12-13 0.0 0.0 0.0 7.419608541453352E-5 0.0 14-15 0.0 0.0 0.0 9.27451067681669E-5 0.0 16-17 0.0 0.0 0.0 1.854902135363338E-4 0.0 18-19 0.0 0.0 0.0 2.4113727759723396E-4 0.0 20-21 0.0 0.0 0.0 3.8952944842630103E-4 0.0 22-23 0.0 0.0 0.0 7.048628114380685E-4 0.0 24-25 0.0 0.0 0.0 0.0012798824734007034 0.0 26-27 0.0 0.0 0.0 0.0017250589858879045 0.0 28-29 0.0 0.0 0.0 0.002856549288459541 0.0 30-31 0.0 0.0 0.0 0.006028431939930849 0.0 32-33 0.0 0.0 0.0 0.011927020730386265 0.0 34-35 0.0 0.0 0.0 0.019105491994242385 0.0 36-37 0.0 0.0 0.0 0.031830120642834886 0.0 38-39 0.0 0.0 0.0 0.05527608363382748 0.0 40-41 0.0 0.0 0.0 0.08324800783510662 0.0 42-43 0.0 0.0 0.0 0.11031102999005772 0.0 44-45 0.0 0.0 0.0 0.14204840552612447 0.0 46-47 0.0 0.0 0.0 0.18092715428334 0.0 48-49 0.0 0.0 0.0 0.2274851978809598 3.709804270726676E-5 50-51 0.0 0.0 0.0 0.28120316372108206 3.709804270726676E-5 52-53 0.0 0.0 0.0 0.3311556782264168 3.709804270726676E-5 54-55 0.0 0.0 0.0 0.3870067815222069 3.709804270726676E-5 56-57 0.0 0.0 0.0 0.4536905132885189 3.709804270726676E-5 58-59 0.0 0.0 0.0 0.5315407559097183 3.709804270726676E-5 60-61 3.709804270726676E-5 0.0 0.0 0.6235995488878007 3.709804270726676E-5 62-63 3.709804270726676E-5 0.0 0.0 0.7140816750508243 3.709804270726676E-5 64-65 3.709804270726676E-5 0.0 0.0 0.8039145854664709 3.709804270726676E-5 66-67 3.709804270726676E-5 0.0 0.0 0.9094399679472911 3.709804270726676E-5 68-69 3.709804270726676E-5 0.0 0.0 1.037502411372776 3.709804270726676E-5 70-71 3.709804270726676E-5 1.854902135363338E-5 0.0 1.1882874059564617 3.709804270726676E-5 72-73 3.709804270726676E-5 3.709804270726676E-5 0.0 1.3413168321239373 3.709804270726676E-5 74-75 3.709804270726676E-5 3.709804270726676E-5 0.0 1.4941051210138152 3.709804270726676E-5 76-77 3.709804270726676E-5 3.709804270726676E-5 0.0 1.663105254566769 3.709804270726676E-5 78-79 3.709804270726676E-5 3.709804270726676E-5 0.0 1.8634903322500704 3.709804270726676E-5 80-81 3.709804270726676E-5 3.709804270726676E-5 0.0 2.092385255753906 3.709804270726676E-5 82-83 3.709804270726676E-5 3.709804270726676E-5 0.0 2.3315934351303627 3.709804270726676E-5 84-85 3.709804270726676E-5 3.709804270726676E-5 0.0 2.5724339283859385 3.709804270726676E-5 86-87 3.709804270726676E-5 3.709804270726676E-5 0.0 2.824236893261512 3.709804270726676E-5 88-89 3.709804270726676E-5 3.709804270726676E-5 0.0 3.110522488833489 3.709804270726676E-5 90-91 3.709804270726676E-5 3.709804270726676E-5 0.0 3.4309753817388593 3.709804270726676E-5 92-93 3.709804270726676E-5 3.709804270726676E-5 0.0 3.7851318464437815 3.709804270726676E-5 94-95 3.709804270726676E-5 3.709804270726676E-5 0.0 4.129754114172936 3.709804270726676E-5 96-97 3.709804270726676E-5 3.709804270726676E-5 0.0 4.48238955912686 3.709804270726676E-5 98-99 3.709804270726676E-5 3.709804270726676E-5 0.0 4.866706732552791 3.709804270726676E-5 100-101 3.709804270726676E-5 3.709804270726676E-5 0.0 5.3009578714627015 3.709804270726676E-5 102-103 3.709804270726676E-5 3.709804270726676E-5 0.0 5.776851563311519 3.709804270726676E-5 104-105 3.709804270726676E-5 5.5647064060900144E-5 0.0 6.251558117793705 3.709804270726676E-5 106-107 3.709804270726676E-5 7.419608541453352E-5 0.0 6.726542907596195 3.709804270726676E-5 108-109 3.709804270726676E-5 7.419608541453352E-5 0.0 7.219149267684637 3.709804270726676E-5 110-111 3.709804270726676E-5 7.419608541453352E-5 0.0 7.774061790499934 3.709804270726676E-5 112-113 3.709804270726676E-5 7.419608541453352E-5 0.0 8.376218670702933 3.709804270726676E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4635 0.0 59.457108 1 GTATCAA 8690 0.0 55.343178 1 ATCAACG 10475 0.0 43.452084 3 TCAACGC 10735 0.0 42.286777 4 CAACGCA 10880 0.0 41.77797 5 AACGCAG 11020 0.0 40.25395 6 TATCAAC 11860 0.0 40.13289 2 GTACATG 14935 0.0 37.46236 1 TACATGG 15135 0.0 36.205315 2 ACGCAGA 13075 0.0 33.38137 7 CGCAGAG 12915 0.0 32.96687 8 ACATGGG 15630 0.0 32.62309 3 CGCATAG 380 0.0 29.773924 8 GCAGAGT 13810 0.0 29.49365 9 CATGGGA 9350 0.0 27.715921 4 ACGCATA 420 0.0 26.936811 7 GAGTACT 6130 0.0 26.200756 12-13 CATGGGG 6745 0.0 25.878395 4 GAGTACA 11260 0.0 25.320347 1 AACGCAT 525 0.0 24.95153 6 >>END_MODULE