FastQCFastQC Report
Thu 26 May 2016
SRR1364508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2695560
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94310.3498716407722328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93180.3456795619463117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54840.20344566620665092No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66700.070.206311
GTATCAA120800.052.4432871
ATCAACG155300.039.1049043
TCAACGC157100.038.1257484
TATCAAC165850.037.7313462
CAACGCA159000.037.5663385
AACGCAG161850.036.2064366
GTACATG135750.033.045351
TACATGG139250.031.7568342
TGGTATC27950.031.3446922
CGCAGAG192250.030.5328968
ACGCAGA190600.030.4647227
ACATGGG141650.028.5260493
GTGGTAT32350.028.2130131
GCAGAGT206000.027.5999229
GAGTACT126850.027.59341412-13
GTACTTT141750.025.61604314-15
CAGAGTA202300.025.29906310-11
AGTACTT133000.024.97589112-13
AGAGTAC195650.024.56297110-11
Produced by FastQC (version 0.11.5)