##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364508_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2695560 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.926165620501862 31.0 28.0 31.0 25.0 34.0 2 29.076353707578388 31.0 28.0 33.0 25.0 34.0 3 29.14545548976836 31.0 28.0 34.0 25.0 34.0 4 32.570638754099335 35.0 32.0 37.0 26.0 37.0 5 31.73202711124961 35.0 32.0 37.0 20.0 37.0 6 31.794417486533412 35.0 32.0 37.0 21.0 37.0 7 31.243179153867843 35.0 31.0 37.0 17.0 37.0 8 31.349670198400332 35.0 32.0 37.0 17.0 37.0 9 31.895156850524565 37.0 30.0 39.0 11.0 39.0 10-11 32.096476613393875 37.0 31.0 39.0 15.5 39.0 12-13 31.84347167193459 37.0 30.5 39.0 11.5 39.0 14-15 32.77487182626245 38.0 31.0 40.0 12.0 41.0 16-17 32.863589940494734 38.0 31.0 40.0 13.0 41.0 18-19 32.774199980709014 38.0 31.0 40.0 11.0 41.0 20-21 32.687871722387925 38.0 31.0 40.0 10.5 41.0 22-23 32.64196382198875 38.0 31.0 40.0 10.5 41.0 24-25 32.490465432043806 38.0 30.5 40.0 10.0 41.0 26-27 32.52685898292006 37.0 31.0 40.0 10.0 41.0 28-29 32.38441789461188 37.0 30.5 40.0 9.5 41.0 30-31 31.8426976212735 37.0 30.0 40.0 8.0 41.0 32-33 31.74573465253973 37.0 30.0 40.0 8.0 41.0 34-35 31.5709301592248 36.0 30.0 40.0 3.5 41.0 36-37 31.487286686254436 36.0 30.0 40.0 2.0 41.0 38-39 31.26219282820638 36.0 30.0 40.0 2.0 41.0 40-41 30.92519198237101 36.0 29.0 40.0 2.0 41.0 42-43 30.78331478431198 36.0 28.5 40.0 2.0 41.0 44-45 30.65063103770645 36.0 28.5 40.0 2.0 41.0 46-47 30.41308689103563 36.0 28.0 40.0 2.0 41.0 48-49 30.076033551469823 36.0 27.0 40.0 2.0 41.0 50-51 29.73895294484263 35.0 26.5 40.0 2.0 41.0 52-53 29.617807245989702 35.0 26.5 40.0 2.0 41.0 54-55 29.139500697443204 35.0 25.5 39.5 2.0 41.0 56-57 29.146238443959696 35.0 26.0 39.0 2.0 41.0 58-59 28.937555461573847 35.0 26.0 39.0 2.0 41.0 60-61 28.669321402602797 34.5 25.5 39.0 2.0 41.0 62-63 28.40629275549422 34.0 24.5 39.0 2.0 40.5 64-65 27.930629627980828 34.0 23.0 38.0 2.0 40.0 66-67 27.576967123714553 34.0 22.0 38.0 2.0 40.0 68-69 27.395539331344878 34.0 22.0 37.0 2.0 40.0 70-71 27.453829259968245 34.0 23.5 37.0 2.0 40.0 72-73 27.253999169003844 34.0 22.5 36.5 2.0 39.0 74-75 26.882234860288772 33.0 22.0 36.0 2.0 39.0 76-77 26.780724042499518 33.0 22.5 36.0 2.0 39.0 78-79 26.398952351273948 33.0 21.0 35.0 2.0 37.5 80-81 26.175423103177074 33.0 20.5 35.0 2.0 37.0 82-83 26.02781314457849 33.0 20.5 35.0 2.0 36.5 84-85 25.63420050008162 33.0 19.0 35.0 2.0 36.0 86-87 25.513111932214457 33.0 19.5 35.0 2.0 36.0 88-89 25.445916618439213 33.0 19.5 35.0 2.0 35.5 90-91 25.286319725771268 33.0 18.5 35.0 2.0 35.0 92-93 25.31645799017644 33.0 19.0 35.0 2.0 35.0 94-95 25.299717869385212 33.0 19.0 35.0 2.0 35.0 96-97 25.18194679398715 33.0 18.5 35.0 2.0 35.0 98-99 25.069566806155308 33.0 18.0 35.0 2.0 35.0 100-101 24.993581111160573 33.0 17.5 35.0 2.0 35.0 102-103 23.850557769072104 30.5 13.0 34.0 2.0 35.0 104-105 24.774404947394977 32.0 17.0 35.0 2.0 35.0 106-107 24.901642886821293 32.5 17.0 35.0 2.0 35.0 108-109 24.958435723931203 33.0 17.0 35.0 2.0 35.0 110-111 24.900628440843462 33.0 15.0 35.0 2.0 35.0 112-113 24.8318666622149 33.0 10.0 35.0 2.0 35.0 114-115 24.772773189986495 33.0 7.5 35.0 2.0 35.0 116-117 24.689774110017957 33.0 3.5 35.0 2.0 35.0 118-119 24.56845015506982 33.0 2.0 35.0 2.0 35.0 120-121 24.403148881864993 33.0 2.0 35.0 2.0 35.0 122-123 24.111680875217022 32.5 2.0 35.0 2.0 35.0 124-125 23.3469661220674 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8078727515558874 1101 2 0.8860807187816775 1101 3 0.8665921843676863 1101 4 0.9023539973015744 1101 5 1.0295977948274135 1101 6 1.0359602910935202 1101 7 1.472097225410483 1101 8 1.3431180269187522 1101 9 2.0359115948096225 1101 10-11 1.6600974341809263 1101 12-13 1.8632630576003848 1101 14-15 1.800664007269333 1101 16-17 1.7864783604858587 1101 18-19 1.8303564439680002 1101 20-21 1.9978141200803137 1101 22-23 1.9839666309315263 1101 24-25 2.094451728816722 1101 26-27 1.8441491053164043 1101 28-29 1.9740474916339537 1101 30-31 2.372998866051166 1101 32-33 2.094130322557586 1101 34-35 2.2234795438580797 1101 36-37 2.096603765147215 1101 38-39 2.2030364689533712 1101 40-41 2.3010614917044236 1101 42-43 2.2143014739826405 1101 44-45 2.057894672621803 1101 46-47 1.9147413327638922 1101 48-49 1.896557694051289 1101 50-51 2.2028983479356796 1101 52-53 1.9606503028335567 1101 54-55 2.38292810894605 1101 56-57 2.147534219725042 1101 58-59 2.275293996265411 1101 60-61 2.3360528748450413 1101 62-63 2.3413818047432216 1101 64-65 2.468180449758819 1101 66-67 2.520434831339074 1101 68-69 2.4720383201710447 1101 70-71 2.0937822957486176 1101 72-73 2.3016804339177455 1101 74-75 2.3039178888440546 1101 76-77 2.080129725631757 1101 78-79 2.2086337763373542 1101 80-81 2.298437936072137 1101 82-83 2.1694587022797123 1101 84-85 2.49297792623031 1101 86-87 2.347074887512086 1101 88-89 2.1386582269464363 1101 90-91 2.096415085039286 1101 92-93 2.0476492349640303 1101 94-95 1.9690441279235138 1101 96-97 1.9301696502977705 1101 98-99 2.0270684659907516 1101 100-101 2.1299525373036836 1101 102-103 1.99410505992455 1101 104-105 1.9922468895157337 1101 106-107 2.0963875671136663 1101 108-109 2.1440084726653907 1101 110-111 2.127859951131242 1101 112-113 2.0826594954625968 1101 114-115 2.0405756719831665 1101 116-117 2.0646955732457712 1101 118-119 2.014929802930361 1101 120-121 1.9708766637833328 1101 122-123 2.0274402261404028 1101 124-125 1.8194473506270796 1102 1 0.4673410861082239 1102 2 -1.5993134859251157 1102 3 -0.06546004641147007 1102 4 0.4673032293284223 1102 5 0.7999000363625051 1102 6 0.509153510557443 1102 7 1.2348809667519625 1102 8 0.9786948672577793 1102 9 1.7380265326403652 1102 10-11 1.4570533994177737 1102 12-13 1.611097029118696 1102 14-15 1.5579521692966125 1102 16-17 1.4272726318794042 1102 18-19 1.6658175505377173 1102 20-21 1.7987992016102297 1102 22-23 1.6687642581741429 1102 24-25 1.8650040994229258 1102 26-27 1.7197717659023866 1102 28-29 1.557731772922935 1102 30-31 1.8841410378878543 1102 32-33 1.5739747380805404 1102 34-35 1.7948935717374503 1102 36-37 1.1937494111481541 1102 38-39 1.1407223851431922 1102 40-41 1.34381855834814 1102 42-43 1.2253887905940992 1102 44-45 1.2270049961600726 1102 46-47 0.8491299659007474 1102 48-49 0.8171097419796958 1102 50-51 1.0340069612816336 1102 52-53 0.8004021636088439 1102 54-55 1.1499630764631519 1102 56-57 0.6331253654463325 1102 58-59 0.6143778064448178 1102 60-61 0.7556544753269101 1102 62-63 1.0014634960051083 1102 64-65 1.1684874776812038 1102 66-67 1.1559889766731537 1102 68-69 1.0658906628118423 1102 70-71 0.4790303274140655 1102 72-73 0.8803832154003466 1102 74-75 1.1033824588221108 1102 76-77 1.0580598013534797 1102 78-79 0.8693715791166845 1102 80-81 0.8462359290162489 1102 82-83 0.5705973246606426 1102 84-85 0.6387311637641915 1102 86-87 0.6515175691796848 1102 88-89 0.8280116344239552 1102 90-91 0.6190829850521915 1102 92-93 0.6752146309972424 1102 94-95 0.5821337678150975 1102 96-97 0.7214036239672623 1102 98-99 0.5164972187335124 1102 100-101 0.37898418123949185 1102 102-103 0.22346293111156967 1102 104-105 0.492403657144699 1102 106-107 0.6897789107440069 1102 108-109 0.6238536411031994 1102 110-111 0.5472536395535599 1102 112-113 0.5092251819058227 1102 114-115 0.7610960437079832 1102 116-117 0.41813085483592616 1102 118-119 0.04013782254917331 1102 120-121 0.34899738830254634 1102 122-123 0.6243022921387684 1102 124-125 0.814498118600234 1103 1 0.7074540584520079 1103 2 0.7301289168113563 1103 3 0.6759487126131063 1103 4 0.6643519604723807 1103 5 0.9271687720897539 1103 6 0.9549888482702222 1103 7 1.2194833292472076 1103 8 1.2197251475073543 1103 9 1.7638698813790192 1103 10-11 1.4821077638147102 1103 12-13 1.4571170294173257 1103 14-15 1.2305275085371576 1103 16-17 1.3099910075324601 1103 18-19 1.5370271635090873 1103 20-21 1.6034934961454965 1103 22-23 1.53142158167352 1103 24-25 1.8665628087518797 1103 26-27 1.3566050432970371 1103 28-29 1.4198346325908489 1103 30-31 1.9124893469922633 1103 32-33 1.8979546252553412 1103 34-35 1.9308723505255792 1103 36-37 1.741142516861892 1103 38-39 1.8755950277927909 1103 40-41 2.1417501685968077 1103 42-43 2.0429811721993794 1103 44-45 1.8667234438734077 1103 46-47 1.8802505400632192 1103 48-49 1.8045255932074582 1103 50-51 2.0138802866645307 1103 52-53 1.8601867163514534 1103 54-55 2.0913261323903676 1103 56-57 1.9243000259352918 1103 58-59 1.9708160896270108 1103 60-61 1.9869736047781181 1103 62-63 2.199743040558161 1103 64-65 2.2359565128589587 1103 66-67 2.3650773470309723 1103 68-69 2.46858153004759 1103 70-71 2.1492518056625727 1103 72-73 2.2092265223743297 1103 74-75 2.210464631995734 1103 76-77 2.165981920419135 1103 78-79 2.1487175877020306 1103 80-81 2.1367035656186317 1103 82-83 1.9924139751732568 1103 84-85 2.2173100333093316 1103 86-87 2.1102088505997543 1103 88-89 2.028814262611732 1103 90-91 2.1259649042187334 1103 92-93 1.994442164256963 1103 94-95 1.7497714630027055 1103 96-97 1.7418449942724976 1103 98-99 1.8975721655376603 1103 100-101 1.8751101837267647 1103 102-103 1.760359580438326 1103 104-105 1.7152892992093811 1103 106-107 1.6692376731119225 1103 108-109 1.7534291714225105 1103 110-111 1.7800935006178733 1103 112-113 1.6368831621708289 1103 114-115 1.5337562431266996 1103 116-117 1.5960473107857815 1103 118-119 1.6289128432096973 1103 120-121 1.6089067589739265 1103 122-123 1.659185040539537 1103 124-125 1.4745595840726509 1104 1 0.6490353468505852 1104 2 0.5526617910007126 1104 3 0.5839526237479653 1104 4 0.5353112069089221 1104 5 0.885123247733393 1104 6 0.8667857685916722 1104 7 1.2968838882517026 1104 8 1.1743757119749034 1104 9 1.6986255467728988 1104 10-11 1.2201304286886767 1104 12-13 1.2541590326803131 1104 14-15 1.4289498298777445 1104 16-17 1.2595709295489002 1104 18-19 1.1386106041450645 1104 20-21 1.516856767018588 1104 22-23 1.5430076383911526 1104 24-25 1.6838019829359183 1104 26-27 1.5705973797651325 1104 28-29 1.627941970689605 1104 30-31 2.029018416834024 1104 32-33 1.924153992530531 1104 34-35 1.9829570625071575 1104 36-37 1.8885277958054658 1104 38-39 2.028652400587106 1104 40-41 2.055949802221683 1104 42-43 2.002067479392924 1104 44-45 1.8330263432505411 1104 46-47 1.8729682366084681 1104 48-49 1.609117022684778 1104 50-51 1.9910611071782398 1104 52-53 1.8285007413940946 1104 54-55 2.0906413436374187 1104 56-57 2.0765620088880894 1104 58-59 2.150886257926203 1104 60-61 2.255587262909639 1104 62-63 2.2259972445333673 1104 64-65 2.346795192770543 1104 66-67 2.295095049389733 1104 68-69 2.400311539287369 1104 70-71 2.253919192215541 1104 72-73 2.370905677790198 1104 74-75 2.3993306275931303 1104 76-77 2.260189464134914 1104 78-79 2.3131340427526332 1104 80-81 2.142080345286004 1104 82-83 2.091540020420542 1104 84-85 2.385975507999312 1104 86-87 2.2627029103008383 1104 88-89 2.113431671491071 1104 90-91 2.2449547194355475 1104 92-93 1.9685429557608138 1104 94-95 1.8757129122500977 1104 96-97 1.9385569156473892 1104 98-99 2.038756104321763 1104 100-101 2.0347133783562334 1104 102-103 1.9302264633182702 1104 104-105 1.8675230392001367 1104 106-107 2.019045981737815 1104 108-109 1.8574039354082394 1104 110-111 1.9093565902000158 1104 112-113 1.882312028457104 1104 114-115 1.757938980451069 1104 116-117 1.783891921307088 1104 118-119 1.8936907669438021 1104 120-121 1.8631325863999422 1104 122-123 2.0626845084586236 1104 124-125 1.8335054701450737 1105 1 0.5089723311670262 1105 2 0.601189757142162 1105 3 0.43063317228150666 1105 4 0.5813529463294245 1105 5 0.8711821353423908 1105 6 0.9414411108517946 1105 7 1.2368016394935104 1105 8 1.1477331083690956 1105 9 1.659457312098116 1105 10-11 1.3650461992886562 1105 12-13 1.5146529682677539 1105 14-15 1.4533249373796835 1105 16-17 1.4258720809272702 1105 18-19 1.4841254928382028 1105 20-21 1.8009676933856227 1105 22-23 1.777191144856907 1105 24-25 1.9353680451593362 1105 26-27 1.5623453743652789 1105 28-29 1.6452344333049922 1105 30-31 2.045598708405347 1105 32-33 2.020062403482438 1105 34-35 2.131262649480785 1105 36-37 1.9073190830662838 1105 38-39 2.0556317133043933 1105 40-41 2.1036797260712135 1105 42-43 2.15185927745085 1105 44-45 2.069395592222431 1105 46-47 1.7803508966104538 1105 48-49 1.802745949437515 1105 50-51 1.8566122207680493 1105 52-53 1.8484708493390762 1105 54-55 2.160242218247152 1105 56-57 1.9785615450009217 1105 58-59 1.8730843273216387 1105 60-61 1.7816913404257058 1105 62-63 1.6442266549796898 1105 64-65 2.1140980798166247 1105 66-67 2.294262997392675 1105 68-69 2.338454505142142 1105 70-71 2.1959367886545564 1105 72-73 2.3504264927721863 1105 74-75 2.382843269821457 1105 76-77 2.177477991527713 1105 78-79 2.2895763669837024 1105 80-81 2.2255970269812266 1105 82-83 2.0896452498362024 1105 84-85 2.3367942688472354 1105 86-87 2.3631317713796633 1105 88-89 2.266049845443547 1105 90-91 2.254843881676173 1105 92-93 2.1345449259782185 1105 94-95 1.986701773745903 1105 96-97 1.993394164223151 1105 98-99 2.0706588495955884 1105 100-101 2.040405769043044 1105 102-103 1.8702348549849788 1105 104-105 1.5540749241978133 1105 106-107 1.7474989701614838 1105 108-109 1.8743060291551465 1105 110-111 2.0592091366278247 1105 112-113 1.9958971465398427 1105 114-115 1.8934159242164803 1105 116-117 1.8491103131301614 1105 118-119 1.8858914424469155 1105 120-121 1.9523953185389864 1105 122-123 1.9436908829979949 1105 124-125 1.8648414651830478 1106 1 0.39914865491941853 1106 2 0.3012304115860083 1106 3 0.35521136587346547 1106 4 0.2358659206588598 1106 5 0.46973473120146636 1106 6 0.6606327892711725 1106 7 0.8385642148929371 1106 8 0.6072451226180142 1106 9 1.3033548417116343 1106 10-11 0.970869849051283 1106 12-13 0.8331115424488686 1106 14-15 1.0006534636892042 1106 16-17 0.9567669421790868 1106 18-19 1.0118281194860685 1106 20-21 0.8707805107915476 1106 22-23 0.785362158370873 1106 24-25 0.7343714153730048 1106 26-27 0.7384735351820666 1106 28-29 0.707169823197006 1106 30-31 1.2442086442454787 1106 32-33 1.0696873099573487 1106 34-35 0.9984796740257416 1106 36-37 1.0246893894312876 1106 38-39 1.0875239988919319 1106 40-41 1.2544981781565738 1106 42-43 1.4308278199162068 1106 44-45 1.2589132345295404 1106 46-47 1.1131579733970263 1106 48-49 1.0145928132848567 1106 50-51 1.437128015747824 1106 52-53 1.2140869892857076 1106 54-55 1.6827400166832156 1106 56-57 1.2801016465708202 1106 58-59 1.2322944128019095 1106 60-61 1.476743284092155 1106 62-63 1.4881878809693987 1106 64-65 1.6000805070325868 1106 66-67 1.589677255581499 1106 68-69 1.553263978819384 1106 70-71 1.4177872941866063 1106 72-73 1.4059383481112278 1106 74-75 1.7601143899374598 1106 76-77 1.2748791528940728 1106 78-79 1.266030293050882 1106 80-81 1.4143255493179936 1106 82-83 1.3331799352277507 1106 84-85 1.5527188925949886 1106 86-87 1.652261938433675 1106 88-89 1.3793268981950035 1106 90-91 1.525486208646491 1106 92-93 1.3987949706716236 1106 94-95 1.2796754410548274 1106 96-97 1.2820233678860227 1106 98-99 1.2830241569206677 1106 100-101 1.0989249938276906 1106 102-103 0.9019375081178183 1106 104-105 1.012901242322151 1106 106-107 1.0040148710811785 1106 108-109 0.8702285341045268 1106 110-111 0.9278461533597095 1106 112-113 0.6622665193093091 1106 114-115 0.8621371631838279 1106 116-117 1.0172829157429106 1106 118-119 0.8303687282661905 1106 120-121 0.8026594399991005 1106 122-123 0.9973522328049853 1106 124-125 0.907332907223978 1107 1 0.5893435127724906 1107 2 0.6867028831238038 1107 3 0.4311897309985042 1107 4 0.5276783125427116 1107 5 0.5667461732512145 1107 6 0.8746655507137469 1107 7 0.9327009054642552 1107 8 0.8727117926724048 1107 9 1.582215157236103 1107 10-11 1.1361573400790945 1107 12-13 1.4738154857637227 1107 14-15 1.267241683290166 1107 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-4.75660418142018 2216 104-105 -4.506760522573437 2216 106-107 -4.633350319683515 2216 108-109 -4.647204858648205 2216 110-111 -4.307507623720937 2216 112-113 -4.344005978514129 2216 114-115 -4.323280390364051 2216 116-117 -4.139059597979234 2216 118-119 -4.370189187479369 2216 120-121 -4.255548400465219 2216 122-123 -4.281906528001802 2216 124-125 -4.294119958873345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 134998.0 3 44191.0 4 21004.0 5 17938.0 6 18967.0 7 22683.0 8 24931.0 9 26191.0 10 28067.0 11 30325.0 12 33540.0 13 36950.0 14 39049.0 15 31492.0 16 20947.0 17 17952.0 18 15745.0 19 14563.0 20 14317.0 21 15163.0 22 18388.0 23 25278.0 24 34801.0 25 46419.0 26 56764.0 27 62948.0 28 71536.0 29 81344.0 30 90776.0 31 102769.0 32 121164.0 33 143627.0 34 173607.0 35 221933.0 36 308113.0 37 391408.0 38 135107.0 39 565.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28863573569737 20.239179260782116 14.13636849782679 23.335816505693717 2 15.947291003151484 21.553780204818672 38.10057669014359 24.398352101886253 3 19.329452878066153 25.83229458813753 29.776781077030375 25.061471456765943 4 12.773706350262362 17.86895396730651 36.35614216488278 33.001197517548356 5 12.93096796213032 39.334943388386826 33.400629182804316 14.333459466678539 6 31.04264690770317 37.65201426200858 17.028824883057393 14.276513947230866 7 27.27060170949941 33.77922139134443 20.522009313138415 18.42816758601775 8 26.400896288711806 35.43015180519076 19.249988870587188 18.91896303551025 9 26.15679116769799 16.076177120895103 20.042477258899822 37.724554452507086 10-11 25.042709391020658 27.377860416250083 26.479377253488444 21.10005293924081 12-13 25.912505972820327 24.857653965210734 27.4665522061595 21.76328785580944 14-15 22.904541717738606 25.696297296575388 26.127638799440856 25.27152218624515 16-17 21.89002823836422 29.236770234541847 26.57949248853576 22.293709038558177 18-19 21.69283295189754 26.826894432496623 30.021791737911503 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22.176473719776173 26.39656439196011 29.07475307772911 22.352208810534606 46-47 22.249338910916947 26.65784225271855 28.520651799705586 22.57216703665891 48-49 22.56708163316032 26.850349037064298 28.050063085245025 22.532506244530357 50-51 22.336109379175202 26.953530922478546 27.778383955345742 22.931975743000503 52-53 22.730896534224083 27.00175622264481 27.612149321216613 22.655197921914496 54-55 22.350361291995483 26.833587989302593 27.695851986743776 23.120198731958155 56-57 22.41489389723125 27.29961673827242 27.58590371453364 22.699585649962696 58-59 22.497273293861017 26.767462048702313 27.743122022882073 22.992142634554604 60-61 22.559820593865467 26.89845523750167 27.937404472540027 22.604319696092833 62-63 22.38873356060953 26.843063862212674 27.985838116997098 22.782364460180695 64-65 22.25465887964169 26.57029954136435 27.828554788224135 23.346486790769823 66-67 22.272350530280807 27.101682659792566 27.530776028884297 23.095190781042334 68-69 22.307808883168274 26.803686941282244 27.701178272711303 23.18732590283818 70-71 22.112046234342568 26.950562338398775 27.90961034663329 23.027781080625363 72-73 22.377644704058028 26.840733328965054 27.680166940055383 23.10145502692153 74-75 22.181954573962326 27.02757048811946 27.453048884661168 23.33742605325705 76-77 22.32018543408991 26.940326386851037 27.66152491195554 23.077963267103517 78-79 22.393492158741317 27.04621654283454 27.421728851695455 23.138562446728688 80-81 21.989753132517585 27.267793224810248 27.57099603402712 23.171457608645046 82-83 22.29807548017468 27.19819718050695 27.335163382835187 23.168563956483183 84-85 22.058518834401543 26.909700935928143 27.257049974226128 23.774730255444183 86-87 22.16389976071674 26.749698577284786 27.650720632152993 23.435681029845487 88-89 22.177926542998623 26.806287092762165 27.428485571906748 23.58730079233247 90-91 21.961710604197545 27.077901155997637 27.589967244167752 23.37042099563707 92-93 22.169910805128172 26.830043748800087 27.599362271042228 23.400683175029517 94-95 22.014137146571336 26.595669297765273 27.650501659832933 23.739691895830457 96-97 22.21394300061992 26.66487479331316 27.469641102235837 23.651541103831082 98-99 21.759727106797847 26.55080947929187 27.44965795604624 24.239805457864044 100-101 21.703634411917083 26.668287871882494 27.71582016196842 23.912257554232006 102-103 22.13102757857353 26.401062653484036 27.561600902832538 23.906308865109896 104-105 21.889233509926626 26.330417852956728 27.755709541658142 24.024639095458504 106-107 21.632177524620595 26.584472197505637 27.75118774087077 24.032162537003 108-109 21.77705189274214 26.641365801540314 27.328551395628363 24.253030910089183 110-111 21.450968113395696 26.57071724084714 27.721019664988837 24.257294980768318 112-113 21.66933525451873 26.41028313989163 27.70524692846528 24.215134677124365 114-115 21.252620515908216 26.66678909034385 27.538130338182427 24.54246005556551 116-117 21.174282894834466 26.57811363872442 27.62952781611242 24.618075650328688 118-119 21.26699828994574 26.622163334574278 27.40121278628662 24.70962558919336 120-121 20.98861637447229 26.538470028412224 27.655797479831623 24.81711611728386 122-123 21.196455537383486 26.546593738482084 27.451573967776632 24.8053767563578 124-125 20.967469360322827 26.418973179627432 27.43770175012582 25.175855709923923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 756.0 1 603.0 2 698.0 3 963.5 4 739.0 5 563.5 6 1019.0 7 2233.0 8 3538.5 9 4191.5 10 3441.5 11 2277.5 12 1993.0 13 2071.0 14 2342.5 15 2651.5 16 2955.5 17 3383.5 18 3925.0 19 4460.0 20 5097.0 21 5880.0 22 6851.0 23 8019.0 24 9389.5 25 11518.0 26 15231.5 27 19628.5 28 23204.0 29 27031.5 30 32229.0 31 37638.0 32 42854.0 33 48687.0 34 54831.0 35 59632.5 36 65018.5 37 71461.5 38 74875.5 39 75918.5 40 77119.0 41 80807.0 42 85900.5 43 89074.0 44 90761.5 45 92835.5 46 93949.5 47 94304.5 48 99465.0 49 103272.0 50 101080.5 51 97271.5 52 93282.0 53 92228.0 54 90086.0 55 82457.0 56 74335.5 57 67648.0 58 61696.0 59 55262.5 60 48003.0 61 39767.5 62 31456.5 63 24464.0 64 19130.0 65 15348.5 66 11992.0 67 9709.0 68 7560.5 69 5360.0 70 4091.5 71 3449.0 72 2988.5 73 2665.0 74 2152.5 75 1714.5 76 1267.0 77 787.5 78 490.0 79 305.5 80 219.5 81 155.5 82 93.5 83 50.0 84 27.5 85 17.0 86 10.5 87 7.5 88 7.5 89 4.0 90 0.5 91 1.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10268738221371441 2 1.854902135363338E-4 3 0.0 4 0.18526762528009022 5 0.0 6 2.5968629895086734E-4 7 0.04032557242279897 8 0.0 9 0.0 10-11 6.30666726023535E-4 12-13 5.935686833162683E-4 14-15 5.935686833162683E-4 16-17 0.004210627847274778 18-19 0.001595215836412471 20-21 0.0031347846087640416 22-23 0.0017250589858879045 24-25 0.004581608274347446 26-27 0.003988039591031178 28-29 0.0031718826514713083 30-31 8.71804003620769E-4 32-33 1.2984314947543367E-4 34-35 0.00126133345204707 36-37 0.001168588345278903 38-39 5.564706406090015E-4 40-41 0.0015581177937052041 42-43 9.27451067681669E-4 44-45 9.460000890353026E-4 46-47 2.9678434165813415E-4 48-49 3.709804270726677E-5 50-51 0.04006588612384811 52-53 7.419608541453354E-5 54-55 0.13700307171793616 56-57 4.822745551944679E-4 58-59 0.0 60-61 0.0 62-63 5.379216192553681E-4 64-65 0.059041534968615056 66-67 0.029993767528825183 68-69 0.0017250589858879045 70-71 0.002763804181691374 72-73 0.07043063407974595 74-75 0.004581608274347446 76-77 0.001743608007241538 78-79 0.0015766668150588375 80-81 1.6694119218270043E-4 82-83 1.6694119218270043E-4 84-85 1.8549021353633385E-5 86-87 3.709804270726676E-4 88-89 0.0013911766015225035 90-91 3.5243140571903427E-4 92-93 0.002133137455667839 94-95 0.0040065886123848106 96-97 0.0021145884343142054 98-99 0.0 100-101 0.0012798824734007034 102-103 0.0016137648577661042 104-105 2.4113727759723396E-4 106-107 5.5647064060900144E-5 108-109 0.0 110-111 2.9678434165813415E-4 112-113 7.419608541453354E-5 114-115 1.1129412812180029E-4 116-117 0.0 118-119 0.001168588345278903 120-121 0.0024299217973259733 122-123 0.001168588345278903 124-125 0.00888498122839039 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2695560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.06553824499963 #Duplication Level Percentage of deduplicated Percentage of total 1 77.27049609943768 39.45859473775929 2 11.240867018881994 11.480418493193469 3 4.37260071374036 6.698676269328632 4 2.222674178926069 4.540082131604892 5 1.2392259747921923 3.1640870704973825 6 0.8018617859089247 2.4568502217321537 7 0.558008592710286 1.9946506392459868 8 0.3863420238790777 1.5783010716838082 9 0.2692251837031968 1.2373316023421383 >10 1.4403355066898027 13.368423217494735 >50 0.11743658973943799 4.170334341810706 >100 0.07623557680047671 7.1074727538200575 >500 0.003722821262385429 1.2950528425968952 >1k 7.44564252475086E-4 0.5283477966204598 >5k 2.2336927574252583E-4 0.9213768102694015 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9431 0.3498716407722328 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9318 0.3456795619463117 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5484 0.20344566620665092 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.5647064060900144E-5 0.0 12-13 0.0 0.0 0.0 1.1129412812180029E-4 0.0 14-15 0.0 0.0 0.0 1.4839217082906705E-4 0.0 16-17 0.0 0.0 0.0 2.5968629895086734E-4 0.0 18-19 0.0 0.0 0.0 4.080784697799344E-4 0.0 20-21 0.0 3.709804270726676E-5 0.0 6.492157473771684E-4 0.0 22-23 0.0 3.709804270726676E-5 0.0 9.830981317425693E-4 0.0 24-25 0.0 3.709804270726676E-5 0.0 0.001558117793705204 0.0 26-27 0.0 3.709804270726676E-5 0.0 0.0020218433275460386 0.0 28-29 0.0 3.709804270726676E-5 0.0 0.0031347846087640416 0.0 30-31 0.0 3.709804270726676E-5 0.0 0.006084079003991749 0.0 32-33 0.0 3.709804270726676E-5 0.0 0.011778628559557199 0.0 34-35 0.0 3.709804270726676E-5 0.0 0.01858611939634065 0.0 36-37 0.0 3.709804270726676E-5 0.0 0.03160753238659128 0.0 38-39 0.0 3.709804270726676E-5 0.0 0.05494220124946208 3.709804270726676E-5 40-41 0.0 3.709804270726676E-5 0.0 0.08274718425855852 3.709804270726676E-5 42-43 0.0 3.709804270726676E-5 0.0 0.10968036326403419 3.709804270726676E-5 44-45 0.0 3.709804270726676E-5 0.0 0.14212260161153897 3.709804270726676E-5 46-47 0.0 3.709804270726676E-5 0.0 0.1812610366677054 3.709804270726676E-5 48-49 0.0 3.709804270726676E-5 0.0 0.22718841353930166 3.709804270726676E-5 50-51 0.0 3.709804270726676E-5 0.0 0.27938535962842603 3.709804270726676E-5 52-53 0.0 3.709804270726676E-5 0.0 0.32905963881345623 3.709804270726676E-5 54-55 0.0 3.709804270726676E-5 0.0 0.3843357224472837 3.709804270726676E-5 56-57 0.0 3.709804270726676E-5 0.0 0.45064847378652306 3.709804270726676E-5 58-59 0.0 3.709804270726676E-5 0.0 0.527960794788467 3.709804270726676E-5 60-61 0.0 7.419608541453352E-5 0.0 0.6180904895457715 3.709804270726676E-5 62-63 0.0 7.419608541453352E-5 0.0 0.704380536882874 3.709804270726676E-5 64-65 0.0 7.419608541453352E-5 0.0 0.7904108979210257 3.709804270726676E-5 66-67 0.0 7.419608541453352E-5 0.0 0.8927087506863138 3.709804270726676E-5 68-69 0.0 7.419608541453352E-5 0.0 1.0171541349478401 3.709804270726676E-5 70-71 0.0 7.419608541453352E-5 0.0 1.163487364406654 3.709804270726676E-5 72-73 0.0 7.419608541453352E-5 0.0 1.309301221267566 3.709804270726676E-5 74-75 0.0 7.419608541453352E-5 0.0 1.4559312350680378 3.709804270726676E-5 76-77 0.0 7.419608541453352E-5 0.0 1.617066583567051 3.709804270726676E-5 78-79 0.0 9.27451067681669E-5 0.0 1.8046342874949919 3.709804270726676E-5 80-81 0.0 1.1129412812180029E-4 0.0 2.0206190921366987 3.709804270726676E-5 82-83 0.0 1.1129412812180029E-4 0.0 2.2484196233806704 3.709804270726676E-5 84-85 0.0 1.1129412812180029E-4 0.0 2.4781863508881274 3.709804270726676E-5 86-87 0.0 1.1129412812180029E-4 0.0 2.718730059802045 3.709804270726676E-5 88-89 0.0 1.1129412812180029E-4 0.0 2.994813693629524 3.709804270726676E-5 90-91 0.0 1.1129412812180029E-4 0.0 3.3062517621570287 3.709804270726676E-5 92-93 0.0 1.1129412812180029E-4 0.0 3.649723990562258 3.709804270726676E-5 94-95 0.0 1.1129412812180029E-4 0.0 3.9868339046431913 3.709804270726676E-5 96-97 0.0 1.1129412812180029E-4 0.0 4.331363427265577 3.709804270726676E-5 98-99 0.0 1.1129412812180029E-4 0.0 4.709967502114589 3.709804270726676E-5 100-101 0.0 1.1129412812180029E-4 0.0 5.142419385953197 3.709804270726676E-5 102-103 0.0 1.1129412812180029E-4 0.0 5.618535666058259 3.709804270726676E-5 104-105 0.0 1.1129412812180029E-4 0.0 6.090626808529581 3.709804270726676E-5 106-107 0.0 1.1129412812180029E-4 0.0 6.5652777159477065 3.709804270726676E-5 108-109 0.0 1.1129412812180029E-4 0.0 7.058496193740818 3.709804270726676E-5 110-111 0.0 1.1129412812180029E-4 0.0 7.6138724420899555 3.709804270726676E-5 112-113 0.0 1.1129412812180029E-4 0.0 8.213302616153971 3.709804270726676E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6670 0.0 70.20631 1 GTATCAA 12080 0.0 52.443287 1 ATCAACG 15530 0.0 39.104904 3 TCAACGC 15710 0.0 38.125748 4 TATCAAC 16585 0.0 37.731346 2 CAACGCA 15900 0.0 37.566338 5 AACGCAG 16185 0.0 36.206436 6 GTACATG 13575 0.0 33.04535 1 TACATGG 13925 0.0 31.756834 2 TGGTATC 2795 0.0 31.344692 2 CGCAGAG 19225 0.0 30.532896 8 ACGCAGA 19060 0.0 30.464722 7 ACATGGG 14165 0.0 28.526049 3 GTGGTAT 3235 0.0 28.213013 1 GCAGAGT 20600 0.0 27.599922 9 GAGTACT 12685 0.0 27.593414 12-13 GTACTTT 14175 0.0 25.616043 14-15 CAGAGTA 20230 0.0 25.299063 10-11 AGTACTT 13300 0.0 24.975891 12-13 AGAGTAC 19565 0.0 24.562971 10-11 >>END_MODULE