FastQCFastQC Report
Thu 26 May 2016
SRR1364507_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364507_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623986
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37400.23029755182618572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29910.18417646457543352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21320.13128191991802887No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18910.11644189050890832No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16550.10190974552736291No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG115600.034.395721
TACATGG114350.033.2998432
GTATCAA104650.032.8756521
ACATGGG115500.030.1350773
CGTAGAC1004.4405455E-429.7487283
ATCAACG117250.027.7570233
TATCAAC125000.027.5116232
TCAACGC118100.027.441624
CAACGCA118050.027.251385
CATGGGA88200.027.1530254
AACGCAG117000.027.021846
GAGTACT38700.025.8235812-13
ATGGGAG32900.024.9887165
ACGCAGA130050.024.127927
CGCAGAG126900.024.1176248
GGGAACG3750.023.8165937
GGTATCA74950.023.7457181
ATGGGAT34650.023.038935
ATATAGG9300.023.0312752
GTATAGG7100.022.635561
Produced by FastQC (version 0.11.5)