##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364507_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1623986 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.847098435577646 31.0 26.0 31.0 10.0 34.0 2 27.08916025138148 31.0 27.0 31.0 10.0 34.0 3 27.103627124864378 31.0 27.0 31.0 10.0 34.0 4 29.88560738824103 35.0 28.0 37.0 10.0 37.0 5 30.624449348701283 35.0 30.0 37.0 16.0 37.0 6 30.82910628539901 35.0 31.0 37.0 13.0 37.0 7 30.719855959349402 35.0 31.0 37.0 11.0 37.0 8 30.303181800828334 35.0 30.0 37.0 10.0 37.0 9 30.968707858318975 37.0 29.0 39.0 10.0 39.0 10-11 31.095475576759895 36.0 29.5 39.0 9.0 39.0 12-13 30.94721690950538 37.0 29.5 39.0 8.0 39.0 14-15 31.677199495562157 37.0 29.5 40.0 8.0 41.0 16-17 31.977446849911267 37.0 30.0 40.0 8.0 41.0 18-19 31.96074658279074 37.0 30.0 40.0 8.0 41.0 20-21 31.760583835082322 37.0 29.5 40.0 8.0 41.0 22-23 31.826806080840598 37.0 30.0 40.0 7.5 41.0 24-25 31.87016975515799 37.0 30.0 40.0 6.5 41.0 26-27 32.00771712317717 37.0 30.0 40.0 2.0 41.0 28-29 31.97113121664842 37.0 30.0 40.0 2.0 41.0 30-31 31.629291754978183 37.0 30.0 40.0 2.0 41.0 32-33 31.64345043614908 37.0 30.0 40.0 2.0 41.0 34-35 31.355402694358204 37.0 30.0 40.0 2.0 41.0 36-37 31.11789787596691 36.5 29.0 40.0 2.0 41.0 38-39 30.978230723663874 36.0 29.0 40.0 2.0 41.0 40-41 31.046237467564374 36.5 29.5 40.0 2.0 41.0 42-43 30.90674550150063 36.0 29.0 40.0 2.0 41.0 44-45 30.576552076187852 36.0 28.5 40.0 2.0 41.0 46-47 30.29796870170063 36.0 27.5 40.0 2.0 41.0 48-49 30.03593349942672 35.5 27.0 40.0 2.0 41.0 50-51 30.025571341132252 36.0 27.5 40.0 2.0 41.0 52-53 29.839838520775423 35.0 27.0 40.0 2.0 41.0 54-55 29.740354596652928 35.0 27.0 40.0 2.0 41.0 56-57 29.33761990559032 35.0 26.0 39.5 2.0 41.0 58-59 29.1124981988761 35.0 26.0 39.0 2.0 41.0 60-61 28.69760145715542 34.0 24.5 39.0 2.0 40.5 62-63 28.657550003509883 34.0 25.5 39.0 2.0 40.0 64-65 28.48270336074326 34.0 25.5 38.5 2.0 40.0 66-67 28.249524626443822 34.0 25.0 38.0 2.0 40.0 68-69 28.080291948329602 34.0 25.5 38.0 2.0 40.0 70-71 27.932832856933494 34.0 25.5 37.5 2.0 40.0 72-73 27.87053121147596 34.0 25.5 37.0 2.0 40.0 74-75 27.563432812844447 34.0 25.0 36.5 2.0 39.0 76-77 27.268831443128203 34.0 25.0 36.0 2.0 39.0 78-79 26.937561653856623 33.0 24.0 36.0 2.0 39.0 80-81 26.905810456494084 33.0 25.0 35.5 2.0 37.5 82-83 26.673558146437223 33.0 25.0 35.0 2.0 37.0 84-85 26.449835774446335 33.0 24.0 35.0 2.0 36.5 86-87 26.208166819172085 33.0 24.0 35.0 2.0 36.0 88-89 25.969764209789986 33.0 23.0 35.0 2.0 36.0 90-91 25.787680435668783 33.0 22.0 35.0 2.0 36.0 92-93 25.67079703889073 33.0 20.5 35.0 2.0 35.0 94-95 25.586897608723227 33.0 20.0 35.0 2.0 35.0 96-97 25.45177760153105 33.0 20.0 35.0 2.0 35.0 98-99 25.329782707486395 33.0 19.5 35.0 2.0 35.0 100-101 25.27159993990096 33.0 19.0 35.0 2.0 35.0 102-103 24.108016017379462 30.5 17.0 34.0 2.0 35.0 104-105 24.890453181246635 32.0 18.0 35.0 2.0 35.0 106-107 24.978937626309587 33.0 17.5 35.0 2.0 35.0 108-109 24.911573437209434 33.0 13.5 35.0 2.0 35.0 110-111 24.786629010348612 33.0 10.0 35.0 2.0 35.0 112-113 24.767504153360928 33.0 9.0 35.0 2.0 35.0 114-115 24.71189776266544 33.0 4.5 35.0 2.0 35.0 116-117 24.531838944424397 33.0 2.0 35.0 2.0 35.0 118-119 24.38444604818022 33.0 2.0 35.0 2.0 35.0 120-121 24.243831227609107 33.0 2.0 35.0 2.0 35.0 122-123 23.970887680066205 32.0 2.0 35.0 2.0 35.0 124-125 22.97701150133068 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.1292923611466428 1101 2 1.71542987701201 1101 3 1.974939136021323 1101 4 2.365459435314925 1101 5 1.879394015504058 1101 6 1.8503865088412716 1101 7 1.6355775346231773 1101 8 2.0438703778058134 1101 9 2.602170522725636 1101 10-11 2.3765075848918045 1101 12-13 2.200362390043537 1101 14-15 2.48239335202296 1101 16-17 2.2172955725048595 1101 18-19 2.241952620039111 1101 20-21 2.5246258444556844 1101 22-23 2.43935808133417 1101 24-25 2.4830049601300814 1101 26-27 2.169784704054276 1101 28-29 2.019762841092117 1101 30-31 2.1891801006506917 1101 32-33 2.2778536991416836 1101 34-35 2.574143907611738 1101 36-37 2.737316979854871 1101 38-39 2.768945073485554 1101 40-41 2.4992416933891164 1101 42-43 2.3683484571637834 1101 44-45 2.484795373646719 1101 46-47 2.3971715825427538 1101 48-49 2.422998374329733 1101 50-51 2.5468969901242957 1101 52-53 2.4096422128777633 1101 54-55 2.400061801499291 1101 56-57 2.6748847626274923 1101 58-59 2.545779832051668 1101 60-61 2.692824106022293 1101 62-63 2.350415690400858 1101 64-65 2.3299962425403926 1101 66-67 2.431968844833168 1101 68-69 2.082772884116469 1101 70-71 2.2725632493825003 1101 72-73 2.1409096276115704 1101 74-75 2.142801901886532 1101 76-77 2.4425094347714165 1101 78-79 2.448271293874207 1101 80-81 2.288745534001915 1101 82-83 2.1336704933233612 1101 84-85 2.0098753234600757 1101 86-87 2.0135647761953557 1101 88-89 2.1200083502751816 1101 90-91 2.1542481726140323 1101 92-93 2.1079819587141984 1101 94-95 2.0814855654305404 1101 96-97 2.1434269514127635 1101 98-99 2.0454110760857382 1101 100-101 2.0301209614992146 1101 102-103 2.083550512024914 1101 104-105 2.0698412199499607 1101 106-107 2.1606678190667417 1101 108-109 2.267735209714459 1101 110-111 2.3187628981451986 1101 112-113 2.169579191018393 1101 114-115 2.1814014659884187 1101 116-117 2.3371450157929274 1101 118-119 2.3337157377381246 1101 120-121 2.3444615102553676 1101 122-123 2.357751756040411 1101 124-125 2.361958615265742 1102 1 1.0934164259273444 1102 2 0.8053119364845323 1102 3 0.9450899939858068 1102 4 1.5316064436078172 1102 5 1.4175069824853068 1102 6 1.3796042424357928 1102 7 1.2762383568744156 1102 8 1.3895765613832118 1102 9 1.8096893180948612 1102 10-11 1.457594184151695 1102 12-13 1.8409501943690643 1102 14-15 2.1639159153330425 1102 16-17 1.5726591670778802 1102 18-19 1.7320636643434817 1102 20-21 1.7535043492582858 1102 22-23 1.7124971689940836 1102 24-25 1.471944633444643 1102 26-27 1.7076368266398951 1102 28-29 1.4313543249967893 1102 30-31 1.3740447568750547 1102 32-33 1.185552795585327 1102 34-35 1.5417618068883279 1102 36-37 1.7342594795106017 1102 38-39 1.8588785616343628 1102 40-41 1.448264309515757 1102 42-43 1.569438205369913 1102 44-45 1.8668232333394172 1102 46-47 1.7781974697491023 1102 48-49 1.7697800109459685 1102 50-51 1.8323562142776098 1102 52-53 1.8530152308497314 1102 54-55 1.6577139952985789 1102 56-57 1.8642181621678588 1102 58-59 1.8737712654374974 1102 60-61 2.364484777302991 1102 62-63 2.049099162550494 1102 64-65 2.1067410432708478 1102 66-67 2.044192847483096 1102 68-69 1.8773239580351309 1102 70-71 2.1285121439281127 1102 72-73 1.8914782344680994 1102 74-75 1.980799952377307 1102 76-77 1.9366089386967253 1102 78-79 1.8181598270373733 1102 80-81 1.4525789458018892 1102 82-83 1.3528986807408678 1102 84-85 1.4094505395568966 1102 86-87 1.4534540603559876 1102 88-89 1.1570609766921898 1102 90-91 1.152901697390547 1102 92-93 1.216059052073362 1102 94-95 1.3566210418258535 1102 96-97 1.4062744438754144 1102 98-99 1.5911035659106467 1102 100-101 1.476251543457483 1102 102-103 1.251595755072831 1102 104-105 1.273842201335551 1102 106-107 1.61747174041464 1102 108-109 1.6301362056573758 1102 110-111 1.3474755204078193 1102 112-113 1.197460550073977 1102 114-115 1.3144227781243991 1102 116-117 1.442714749145651 1102 118-119 1.6853126251235082 1102 120-121 1.6225803125023113 1102 122-123 1.7228861822729513 1102 124-125 1.6280521284827536 1103 1 1.589720777760423 1103 2 1.3583437755282048 1103 3 1.4696407938577885 1103 4 2.048472708229969 1103 5 1.5425306510549568 1103 6 1.5818486093162214 1103 7 1.6745757760033122 1103 8 1.8285820290322583 1103 9 2.5890948976773913 1103 10-11 2.077758464970678 1103 12-13 2.186069777199002 1103 14-15 2.468021427721972 1103 16-17 2.2340587042630844 1103 18-19 2.041603312758401 1103 20-21 2.4504519834905025 1103 22-23 2.312751365242967 1103 24-25 2.4242212566058257 1103 26-27 2.0999310106230133 1103 28-29 1.9599233358432784 1103 30-31 2.331875325105049 1103 32-33 2.1754695660027217 1103 34-35 2.049919713531107 1103 36-37 2.4475958178446326 1103 38-39 2.4564458515873326 1103 40-41 2.569258037554256 1103 42-43 2.511589707674773 1103 44-45 2.4417616950583394 1103 46-47 2.598223341799752 1103 48-49 2.5689046508904703 1103 50-51 2.444427101015336 1103 52-53 2.4288233554109198 1103 54-55 2.329634106963958 1103 56-57 2.451429091433603 1103 58-59 2.735647357752459 1103 60-61 2.753591035356589 1103 62-63 2.570561737724386 1103 64-65 2.403480394090156 1103 66-67 2.425779621905285 1103 68-69 2.176086657374757 1103 70-71 2.436154955469327 1103 72-73 2.4689452534683056 1103 74-75 2.530807241458934 1103 76-77 2.501632573753355 1103 78-79 2.6659830726727236 1103 80-81 2.185021894076222 1103 82-83 2.2752406375325336 1103 84-85 2.1764646803752505 1103 86-87 2.159723340814711 1103 88-89 2.2389408224652776 1103 90-91 2.150229871953158 1103 92-93 2.1362444460943024 1103 94-95 2.074758957952824 1103 96-97 2.2471144141828816 1103 98-99 2.1961869881931158 1103 100-101 2.027322870891407 1103 102-103 2.10825387033416 1103 104-105 2.2370968516858483 1103 106-107 1.9977448044932054 1103 108-109 2.3087497050365435 1103 110-111 2.4243686289581596 1103 112-113 2.2029080913075596 1103 114-115 2.154102360150027 1103 116-117 2.2854088272276343 1103 118-119 2.3571162790479683 1103 120-121 2.3669765168177825 1103 122-123 2.363618240659335 1103 124-125 2.2755077363 1104 1 1.2340722421955697 1104 2 1.0966473129949215 1104 3 1.0713905085782116 1104 4 1.3425008558146025 1104 5 1.00772654302909 1104 6 1.1269227820054546 1104 7 1.0990717820892755 1104 8 1.4067486323034615 1104 9 1.9489275336606582 1104 10-11 1.704612782201572 1104 12-13 1.2048982290818486 1104 14-15 1.5577894003351318 1104 16-17 1.3126056802996047 1104 18-19 0.6209110343255411 1104 20-21 -3.333115913048111 1104 22-23 -0.22813870702785977 1104 24-25 0.8626008686255986 1104 26-27 0.9502543274735267 1104 28-29 0.5892500760486783 1104 30-31 1.2462914529102953 1104 32-33 1.1973410000080413 1104 34-35 1.4607223000659069 1104 36-37 1.4609088646304862 1104 38-39 1.3765675708722256 1104 40-41 1.474735405374723 1104 42-43 1.3835942721479526 1104 44-45 1.6947095839894928 1104 46-47 1.550296373370685 1104 48-49 1.6274820567482102 1104 50-51 1.469721889908449 1104 52-53 1.5537758088152565 1104 54-55 1.5664161533694383 1104 56-57 1.870790065187883 1104 58-59 1.730702511803429 1104 60-61 1.810837136535742 1104 62-63 1.5043768765604426 1104 64-65 1.5021871266881028 1104 66-67 1.2993056774397118 1104 68-69 1.3809173914941937 1104 70-71 1.57708306501668 1104 72-73 1.5320871325097656 1104 74-75 1.5180647538210472 1104 76-77 1.4942153048298117 1104 78-79 1.3784212620983602 1104 80-81 1.2215376795459818 1104 82-83 1.1597975032609504 1104 84-85 1.1256849162063673 1104 86-87 1.1235879281102648 1104 88-89 1.3271873040171975 1104 90-91 1.2698571066431583 1104 92-93 1.0253657850064357 1104 94-95 1.2170183721787637 1104 96-97 1.3703551901433215 1104 98-99 1.2334635191934957 1104 100-101 0.7253068952352599 1104 102-103 1.0569035470173063 1104 104-105 1.2838827018189782 1104 106-107 1.4247132335536854 1104 108-109 1.4884758366455237 1104 110-111 1.4993020637242331 1104 112-113 1.4811507691318297 1104 114-115 1.418841804805787 1104 116-117 1.4698896184029877 1104 118-119 1.4845411554268182 1104 120-121 1.492879902135396 1104 122-123 1.2687152357144882 1104 124-125 1.4337428066689633 1105 1 1.478589367047423 1105 2 1.1835458944541735 1105 3 1.1406969230729125 1105 4 1.757502228974971 1105 5 1.2030221102911831 1105 6 1.2312251063914594 1105 7 1.2708250655580429 1105 8 1.5431699854612155 1105 9 1.8309667428965213 1105 10-11 1.591196050793208 1105 12-13 1.7291513253014728 1105 14-15 1.8459911623862695 1105 16-17 1.5327276198776119 1105 18-19 1.4078373727163118 1105 20-21 1.6337959888010332 1105 22-23 1.471295139686326 1105 24-25 1.530571278855426 1105 26-27 1.418246546720976 1105 28-29 1.1472269628958642 1105 30-31 1.3738625304813716 1105 32-33 1.3466420099785132 1105 34-35 1.2219334568837574 1105 36-37 1.5069446167331826 1105 38-39 1.226491202595021 1105 40-41 1.4866450382048626 1105 42-43 1.6529096452290233 1105 44-45 1.7846137711740155 1105 46-47 2.010136646181671 1105 48-49 1.7065636107251585 1105 50-51 1.7906017846377154 1105 52-53 1.7119770274767276 1105 54-55 1.6505212713657684 1105 56-57 1.9404344093092156 1105 58-59 1.7661812307549525 1105 60-61 2.138368341054395 1105 62-63 1.9430152595502932 1105 64-65 1.6699295335518478 1105 66-67 1.8216273905564044 1105 68-69 1.4745893251242883 1105 70-71 1.5760251440842836 1105 72-73 1.5485551437476524 1105 74-75 1.7627589209001542 1105 76-77 1.6398062117177865 1105 78-79 1.4587376323728876 1105 80-81 1.5136167471480633 1105 82-83 1.618307132272669 1105 84-85 1.4762273512676316 1105 86-87 1.6535834047584572 1105 88-89 1.7627000215382829 1105 90-91 1.7801283462030995 1105 92-93 1.702520875759852 1105 94-95 1.578900982387733 1105 96-97 1.6392563387515828 1105 98-99 1.437963813651951 1105 100-101 1.641746121507122 1105 102-103 1.5622291328868272 1105 104-105 1.644237873052809 1105 106-107 1.7028646366004843 1105 108-109 1.8452232676607387 1105 110-111 1.7850519634908686 1105 112-113 1.7198102268289404 1105 114-115 1.6740098811035722 1105 116-117 1.7907598936665678 1105 118-119 1.8087623091458838 1105 120-121 1.8475763308904014 1105 122-123 1.8661559438246513 1105 124-125 1.433549536182806 1106 1 0.10249955434115776 1106 2 0.21376701111793395 1106 3 0.15313818406573887 1106 4 -0.11751100640906387 1106 5 -0.21923346758432416 1106 6 0.04041993273576594 1106 7 0.03056340015153225 1106 8 0.4175661164042772 1106 9 0.4148757967939858 1106 10-11 -1.9228443428532103 1106 12-13 -0.041374257390970115 1106 14-15 0.22257139267449588 1106 16-17 0.11150470021900105 1106 18-19 -2.584117452494599 1106 20-21 -5.076184982072178 1106 22-23 -5.890513581797947 1106 24-25 -5.823286816949366 1106 26-27 -4.605548884200758 1106 28-29 -1.1530531052868724 1106 30-31 -3.129055293815366 1106 32-33 -3.667089533185244 1106 34-35 -2.929459278344435 1106 36-37 -5.399736232299929 1106 38-39 -5.9051510587691425 1106 40-41 -2.1232547307910856 1106 42-43 -0.7480170553500542 1106 44-45 -0.0775708352168536 1106 46-47 0.27617263747838194 1106 48-49 0.35496171914644137 1106 50-51 0.049686999632548634 1106 52-53 0.02542753310567747 1106 54-55 0.14505546786983814 1106 56-57 0.24662722685776828 1106 58-59 0.505980515223996 1106 60-61 0.509861933637211 1106 62-63 0.2371708122226437 1106 64-65 0.20126026007377007 1106 66-67 -0.07041900114236199 1106 68-69 -0.019034948048076927 1106 70-71 0.39832777281796794 1106 72-73 0.3329813096917782 1106 74-75 0.26113272095414075 1106 76-77 0.2996516969978025 1106 78-79 0.24680699622440727 1106 80-81 -0.002661433035100913 1106 82-83 -0.129190448075434 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-5.339580825046525 2216 80-81 -5.33535825164271 2216 82-83 -5.531791171104924 2216 84-85 -5.315535508549761 2216 86-87 -5.533668880688374 2216 88-89 -5.815582614187239 2216 90-91 -5.800903369795456 2216 92-93 -5.568385450083831 2216 94-95 -8.90044904876775 2216 96-97 -10.888559533758468 2216 98-99 -7.695733047753091 2216 100-101 -6.6743359411851095 2216 102-103 -7.730560577552112 2216 104-105 -10.5021620324849 2216 106-107 -7.104381218581995 2216 108-109 -9.14460852117621 2216 110-111 -6.802701228167994 2216 112-113 -6.277336841283493 2216 114-115 -6.5972925035002135 2216 116-117 -6.2165141294880755 2216 118-119 -6.438226705344974 2216 120-121 -7.033624819728583 2216 122-123 -9.802908367354618 2216 124-125 -8.51224902582799 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 120167.0 3 18538.0 4 11746.0 5 10435.0 6 10214.0 7 10356.0 8 11446.0 9 12223.0 10 13290.0 11 14393.0 12 14941.0 13 16048.0 14 17543.0 15 15049.0 16 11958.0 17 10703.0 18 9968.0 19 9228.0 20 9119.0 21 9741.0 22 11995.0 23 16648.0 24 21993.0 25 27049.0 26 33122.0 27 36738.0 28 42274.0 29 48505.0 30 54441.0 31 61023.0 32 73584.0 33 91087.0 34 113899.0 35 145277.0 36 193153.0 37 222878.0 38 72946.0 39 268.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.213247467606195 19.98251948682736 13.856943326401122 21.947289719165326 2 14.294505017977002 21.7312005262824 41.35850339675127 22.615791058989334 3 18.5134464989204 28.61826481486615 28.885685508090077 23.982603178123373 4 10.408583204470265 17.840583549474175 39.33438888101677 32.416444365038785 5 12.391890176807847 40.62902389105413 33.45154203206421 13.527543900073807 6 28.88709214582438 38.67885605624887 18.633607689020312 13.80044410890644 7 27.17442575060993 34.72018158239252 21.057104115530937 17.048288551466612 8 24.899319684325647 36.66580046700887 20.267963585130595 18.166916263534883 9 26.170082659997867 17.948544259092877 20.758971870475733 35.12240121043352 10-11 23.175167021222517 29.173728258743104 27.38710566893983 20.263999051094554 12-13 24.580844582809 26.740134263979005 27.903636359717606 20.77538479349439 14-15 22.14799054082349 27.72517889913907 26.114532398911212 24.012298161126232 16-17 20.86076247179586 31.372457239183117 26.421739613778435 21.345040675242593 18-19 20.779761466686416 30.005459703660957 28.830653418442715 20.384125411209915 20-21 22.226089476410092 28.912434952030974 28.569715392014206 20.291760179544728 22-23 21.638305485365112 29.06238433460179 28.164281970762868 21.135028209270228 24-25 21.162683710320294 29.043536205036236 28.70857125965049 21.08520882499298 26-27 21.248766459395924 29.152016848509664 28.454521801916087 21.14469489017832 28-29 21.079147987585596 28.785438937878716 28.93652396669787 21.19888910783782 30-31 21.15411259836362 28.998847870222527 28.83902142247078 21.008018108943077 32-33 20.929073266797506 29.210837946236406 28.525476218480723 21.33461256848537 34-35 21.21520158307738 28.87690440131726 28.639787691240787 21.268106324364574 36-37 21.466167346545426 28.563282605931313 28.607526810540897 21.363023236982368 38-39 21.411060210351035 28.70643782057164 28.739538025823702 21.142963943253623 40-41 21.258495387459273 28.681801676609968 28.9929899482862 21.066712987644557 42-43 21.058362221813024 28.874633338527 28.628262694354444 21.43874174530553 44-45 21.093783714137405 29.109317955679508 28.354552818763896 21.442345511419187 46-47 21.170625280569876 28.987817802154897 27.98589468615276 21.85566223112246 48-49 21.429033297020208 29.02984840245862 27.810053829228142 21.73106447129303 50-51 21.602863631115046 29.10893345136831 27.679273315114894 21.608929602401755 52-53 21.44731040292842 29.27999054077689 27.77858520567652 21.494113850618174 54-55 21.36551309198925 29.146930740872 27.918235610543302 21.569320556595446 56-57 21.390686022752885 29.476187309176403 27.44953143147101 21.683595236599704 58-59 21.275808234616676 28.9927667659222 27.774725866894943 21.956699132566182 60-61 21.48246955679723 29.05897563989398 27.801239718547055 21.657315084761734 62-63 21.488333883972818 29.096180450461073 27.899532567118236 21.515953098447874 64-65 21.3845232970805 28.885448554166487 27.99824863151244 21.731779517240575 66-67 21.25012470332924 29.303742824170154 27.73528285178347 21.710849620717134 68-69 21.22622715395856 29.2268910046993 27.901036974269317 21.64584486707282 70-71 21.191600309621737 28.9208746506133 27.982310672281898 21.905214367483065 72-73 21.275131644697403 28.98926614258176 27.600142490009844 22.135459722710994 74-75 21.230982572964667 29.15415361481209 27.703173270951265 21.91169054127198 76-77 21.371973411468247 28.9008952753258 27.95616981287773 21.77096150032823 78-79 21.190219344451812 28.7875619713439 28.06322978677146 21.95898889743283 80-81 20.99247723048284 29.461866273712054 27.6584090572507 21.887247438554404 82-83 21.284888060161045 29.278228815918144 27.607136611187332 21.82974651273348 84-85 21.25947038214668 28.966106908210225 27.576619767485894 22.1978029421572 86-87 21.237203485251968 28.970610467148028 27.85966254056136 21.93252350703864 88-89 21.49863891936263 28.721926653441248 27.885695706029985 21.893738721166137 90-91 21.255771950345757 29.00989864275072 27.989583163347465 21.744746243556058 92-93 21.213019700863146 29.11322408046115 27.772071487906874 21.901684730768828 94-95 20.963359374206203 28.987207817619264 27.727079172238312 22.32235363593622 96-97 21.221928905753046 28.824870362946804 27.56322981458253 22.389970916717623 98-99 21.10252497635375 28.652175947820602 27.74499733979664 22.500301736029005 100-101 21.097201529243222 28.88769631645987 27.816382435193898 22.198719719103007 102-103 21.254395093505423 28.62676034655813 27.946914043978644 22.171930515957808 104-105 21.00940933050889 28.724629292083353 28.07247278191044 22.193488595497314 106-107 20.894792650621255 28.851077665036513 28.010639332895725 22.243490351446514 108-109 20.970166302435576 28.70695191841716 27.67722167110333 22.645660108043934 110-111 20.6947820989342 28.820134634852838 27.917057689039304 22.568025577173653 112-113 20.920361927698824 28.851100934746913 27.989880015308678 22.238657122245584 114-115 20.788501552704194 28.988676312747813 27.808922861113793 22.413899273434197 116-117 20.42351745797155 28.952121436049016 28.172024139417452 22.45233696656198 118-119 20.61999746293858 28.92397806303982 27.838984042376314 22.617040431645286 120-121 20.42112056596082 29.143034674214125 27.780446319813677 22.65539844001138 122-123 20.452770142763057 28.951585813169352 27.79059488661503 22.80504915745256 124-125 20.657030838695327 29.00372102451243 27.57461225593773 22.764635880854513 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3241.0 1 3310.5 2 3801.0 3 3706.0 4 2657.0 5 1775.0 6 1344.0 7 1349.0 8 1620.0 9 1800.5 10 1560.0 11 1299.0 12 1273.5 13 1326.5 14 1450.5 15 1603.5 16 1726.0 17 1854.0 18 2096.0 19 2366.5 20 2769.0 21 3364.0 22 4111.5 23 5189.0 24 6389.0 25 7943.5 26 10246.5 27 12831.5 28 15258.5 29 18306.0 30 22589.5 31 26887.5 32 31010.0 33 35718.0 34 41076.0 35 45879.0 36 51200.5 37 55976.0 38 57333.0 39 57532.5 40 56790.5 41 56118.0 42 55572.0 43 56098.5 44 58228.5 45 58931.0 46 57626.0 47 57253.5 48 60672.5 49 60148.0 50 55517.0 51 51287.0 52 47194.5 53 44457.0 54 41519.0 55 37669.5 56 33812.5 57 30548.0 58 28104.0 59 25650.0 60 22063.5 61 18079.5 62 14299.5 63 10810.5 64 8049.0 65 6265.0 66 4782.5 67 3533.0 68 2622.5 69 1926.5 70 1453.5 71 1172.5 72 998.5 73 897.5 74 747.0 75 567.0 76 416.0 77 277.5 78 180.0 79 128.5 80 94.0 81 66.5 82 43.0 83 35.5 84 34.5 85 22.0 86 13.5 87 12.0 88 11.5 89 7.5 90 6.0 91 6.5 92 3.5 93 1.5 94 2.5 95 2.0 96 0.5 97 1.5 98 2.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09901563190815685 2 0.03220471112435699 3 0.043165396746031065 4 0.050246738580258694 5 0.053448736627039896 6 0.0036946131309013746 7 0.0024630754206009164 8 0.002093614107510779 9 0.006465572979077406 10-11 0.06548701774522687 12-13 0.005880592566684688 14-15 0.010221762995493804 16-17 0.02047431443374512 18-19 0.01671812441732872 20-21 0.12968092089463826 22-23 0.014101106782940246 24-25 0.014408991210515362 26-27 0.006373207650804871 28-29 0.004064074443991513 30-31 0.0022783447640558475 32-33 0.006096111665987268 34-35 0.01927356516620217 36-37 0.0027401714054185195 38-39 0.007943418231437956 40-41 0.03272811465123467 42-43 0.01311587661469988 44-45 0.11650346739442334 46-47 0.003633036245386352 48-49 0.009051802170708369 50-51 0.010683589636856476 52-53 0.010714378079613987 54-55 0.009205744384495926 56-57 0.006403996093562382 58-59 0.014224260553970294 60-61 0.03405201768980767 62-63 0.007173707162500168 64-65 0.006958188063197589 66-67 0.008128148887983026 68-69 0.00782026446040791 70-71 0.00424880510053658 72-73 7.697110689377864E-4 74-75 9.236532827253437E-4 76-77 0.007881841345922933 78-79 0.021705852144045575 80-81 0.003048055832993634 82-83 0.004802997070171787 84-85 0.002555440748873451 86-87 0.0035406709171138173 88-89 0.005695861910139619 90-91 0.004710631741899253 92-93 0.002894113619206077 94-95 0.003971709115718978 96-97 0.0017857296799356646 98-99 0.0030788442757511457 100-101 0.002801748290933542 102-103 9.852301682403667E-4 104-105 0.004064074443991513 106-107 7.38922626180275E-4 108-109 0.0010468070537553895 110-111 0.0023399216495708706 112-113 0.004341170428809115 114-115 0.002770959848176031 116-117 0.005295612154291971 118-119 0.0036946131309013746 120-121 0.007881841345922933 122-123 0.004402747314324138 124-125 0.056127331146943384 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1623986.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.75773404927345 #Duplication Level Percentage of deduplicated Percentage of total 1 81.20262657015729 50.96092842392397 2 10.592323376556049 13.295004268596134 3 3.5738365242872683 6.728576463804005 4 1.6043935457965965 4.027524138298945 5 0.838565485656381 2.6313234865861497 6 0.5114702628309218 1.9259228837312994 7 0.33768313042360637 1.4834559664435567 8 0.22493725785938637 1.1293242085209774 9 0.16315080507811333 0.9215077351515379 >10 0.8598318418728719 9.83922129212475 >50 0.06097215654360714 2.6220485770669306 >100 0.02846238247084475 3.0229802388932336 >500 0.0010274473335896352 0.4604535831554329 >1k 7.192131335127447E-4 0.9517287337031493 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3740 0.23029755182618572 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2991 0.18417646457543352 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2132 0.13128191991802887 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1891 0.11644189050890832 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1655 0.10190974552736291 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.157688551502292E-5 0.0 0.0 0.0 6.157688551502292E-5 8 6.157688551502292E-5 0.0 0.0 0.0 6.157688551502292E-5 9 6.157688551502292E-5 0.0 0.0 0.0 6.157688551502292E-5 10-11 6.157688551502292E-5 0.0 0.0 0.0 6.157688551502292E-5 12-13 6.157688551502292E-5 0.0 0.0 0.0 6.157688551502292E-5 14-15 6.157688551502292E-5 0.0 0.0 0.0 6.157688551502292E-5 16-17 1.2315377103004583E-4 0.0 0.0 0.0 6.157688551502292E-5 18-19 1.2315377103004583E-4 0.0 0.0 9.236532827253437E-5 6.157688551502292E-5 20-21 1.2315377103004583E-4 0.0 0.0 2.4630754206009166E-4 6.157688551502292E-5 22-23 1.2315377103004583E-4 0.0 0.0 7.081341834227635E-4 6.157688551502292E-5 24-25 1.2315377103004583E-4 0.0 0.0 0.0015394221378755729 6.157688551502292E-5 26-27 1.2315377103004583E-4 0.0 0.0 0.0021859794357833133 6.157688551502292E-5 28-29 1.2315377103004583E-4 0.0 0.0 0.003910132230203955 6.157688551502292E-5 30-31 1.2315377103004583E-4 0.0 0.0 0.007204495605257681 6.157688551502292E-5 32-33 1.2315377103004583E-4 0.0 0.0 0.01351612637054753 1.2315377103004583E-4 34-35 1.8473065654506875E-4 0.0 0.0 0.021829005915075625 1.2315377103004583E-4 36-37 2.4630754206009166E-4 0.0 0.0 0.03608405491180343 1.2315377103004583E-4 38-39 2.4630754206009166E-4 0.0 0.0 0.05994509804887481 1.2315377103004583E-4 40-41 2.4630754206009166E-4 0.0 0.0 0.08931727243954074 1.2315377103004583E-4 42-43 4.310381986051604E-4 0.0 0.0 0.11665740960821092 1.2315377103004583E-4 44-45 7.38922626180275E-4 0.0 0.0 0.14766137146502495 1.2315377103004583E-4 46-47 8.004995116952979E-4 0.0 0.0 0.1862085017974293 1.2315377103004583E-4 48-49 8.004995116952979E-4 0.0 0.0 0.23451556848396476 1.2315377103004583E-4 50-51 8.004995116952979E-4 0.0 0.0 0.2907352649591807 1.2315377103004583E-4 52-53 8.004995116952979E-4 0.0 0.0 0.34443030912828065 1.2315377103004583E-4 54-55 8.004995116952979E-4 0.0 0.0 0.399172160351136 1.2315377103004583E-4 56-57 8.004995116952979E-4 0.0 0.0 0.46672200376111617 1.2315377103004583E-4 58-59 8.620763972103208E-4 0.0 0.0 0.5479419157554314 1.2315377103004583E-4 60-61 8.620763972103208E-4 0.0 0.0 0.6437247611740495 1.2315377103004583E-4 62-63 8.620763972103208E-4 0.0 0.0 0.7421554126698137 1.2315377103004583E-4 64-65 8.620763972103208E-4 0.0 0.0 0.8394161033407923 1.2315377103004583E-4 66-67 9.852301682403667E-4 0.0 0.0 0.9477298449617176 1.2315377103004583E-4 68-69 0.0011083839392704124 0.0 0.0 1.0766718432301756 1.2315377103004583E-4 70-71 0.001139172382027924 0.0 0.0 1.2299059228343103 1.2315377103004583E-4 72-73 0.0011699608247854353 0.0 0.0 1.3920994392808805 1.2315377103004583E-4 74-75 0.0011699608247854353 0.0 0.0 1.5519838225206375 1.2315377103004583E-4 76-77 0.0011699608247854353 0.0 0.0 1.7250456592606094 1.2315377103004583E-4 78-79 0.0011699608247854353 0.0 0.0 1.9325905518889943 1.2315377103004583E-4 80-81 0.001293114595815481 0.0 0.0 2.174741654176822 1.2315377103004583E-4 82-83 0.001293114595815481 0.0 0.0 2.426806635032568 1.2315377103004583E-4 84-85 0.001354691481330504 0.0 0.0 2.678379000804194 1.2315377103004583E-4 86-87 0.001416268366845527 0.0 0.0 2.936478516440413 1.2315377103004583E-4 88-89 0.001416268366845527 0.0 0.0 3.222072111459089 1.2315377103004583E-4 90-91 0.00147784525236055 0.0 0.0 3.5583742716993862 1.2315377103004583E-4 92-93 0.00147784525236055 0.0 0.0 3.9327925240734833 1.2315377103004583E-4 94-95 0.00147784525236055 0.0 0.0 4.2950801300011205 1.2315377103004583E-4 96-97 0.00147784525236055 0.0 0.0 4.645021570382996 1.2315377103004583E-4 98-99 0.0016933643516631302 0.0 0.0 5.028460836485044 1.2315377103004583E-4 100-101 0.0018473065654506873 0.0 0.0 5.4920424190848935 1.2315377103004583E-4 102-103 0.002032037221995756 0.0 0.0 5.978592179981847 1.2315377103004583E-4 104-105 0.0026785945199034964 0.0 0.0 6.458891886999027 1.2315377103004583E-4 106-107 0.002832536733691054 0.0 0.0 6.926291236500807 1.2315377103004583E-4 108-109 0.002832536733691054 0.0 0.0 7.414288054207363 1.2315377103004583E-4 110-111 0.0029556905047211 0.0 0.0 7.973590905340316 1.2315377103004583E-4 112-113 0.0029556905047211 0.0 0.0 8.586434858428582 1.2315377103004583E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11560 0.0 34.39572 1 TACATGG 11435 0.0 33.299843 2 GTATCAA 10465 0.0 32.875652 1 ACATGGG 11550 0.0 30.135077 3 CGTAGAC 100 4.4405455E-4 29.748728 3 ATCAACG 11725 0.0 27.757023 3 TATCAAC 12500 0.0 27.511623 2 TCAACGC 11810 0.0 27.44162 4 CAACGCA 11805 0.0 27.25138 5 CATGGGA 8820 0.0 27.153025 4 AACGCAG 11700 0.0 27.02184 6 GAGTACT 3870 0.0 25.82358 12-13 ATGGGAG 3290 0.0 24.988716 5 ACGCAGA 13005 0.0 24.12792 7 CGCAGAG 12690 0.0 24.117624 8 GGGAACG 375 0.0 23.816593 7 GGTATCA 7495 0.0 23.745718 1 ATGGGAT 3465 0.0 23.03893 5 ATATAGG 930 0.0 23.031275 2 GTATAGG 710 0.0 22.63556 1 >>END_MODULE