FastQCFastQC Report
Thu 26 May 2016
SRR1364507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623986
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97800.602221940336924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88750.5464948589458283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59370.36558196930269105No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22310.13737803158401612No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.13072772794839363No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19500.12007492675429468No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19160.11798131264678391No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100050.035.6679041
GTATCAA140900.033.8822671
GTACATG107450.030.5456811
TACATGG109200.029.0912042
TATCAAC174850.027.2015952
GAGTACT100600.027.19526312-13
ACATGGG109150.026.5380883
ATCAACG173550.026.443763
TCAACGC172950.026.2598084
CAACGCA173250.026.1666975
TATAACG2109.655923E-825.5434952
AACGCAG175900.025.366626
GTACTTT108900.025.28637314-15
ATAACGC2651.3515091E-924.7402423
AGTACTT107050.024.22329512-13
CATGGGA85850.023.2574254
CGCAGAG195350.022.9847728
ACGCAGA196500.022.616497
CATGGGG28450.021.7875394
GCAGAGT200100.021.7555799
Produced by FastQC (version 0.11.5)