FastQCFastQC Report
Thu 26 May 2016
SRR1364506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827304
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71700.045.6564871
GGTATCA44550.041.416461
ATCAACG80600.038.3122443
TCAACGC81500.037.9688874
CAACGCA82750.037.6823275
AACGCAG84150.036.618156
GTACATG116400.036.1002921
TATCAAC88050.036.0835722
TACATGG121150.033.5424582
ACATGGG122050.031.3457363
ACGCAGA99550.030.2972227
CGCAGAG99800.029.3873358
GCAGAGT105850.026.3026079
CATGGGA79950.025.9025734
AGTACAT85750.022.8969382
CATGGGG58050.022.4503964
GAGTACA96050.022.3701461
GTATAGG10950.022.2858241
CAGAGTA106100.021.19435710-11
AGAGTAC102500.021.15521810-11
Produced by FastQC (version 0.11.5)