##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364506_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2827304 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.59933456041515 30.0 25.0 31.0 7.0 34.0 2 25.66271295905923 30.0 25.0 31.0 7.0 34.0 3 25.799615464060462 30.0 25.0 31.0 7.0 34.0 4 28.489352754426125 35.0 26.0 35.0 7.0 37.0 5 29.07042574834542 35.0 28.0 37.0 2.0 37.0 6 29.183969958660263 35.0 28.0 37.0 2.0 37.0 7 28.873392461511038 35.0 28.0 37.0 2.0 37.0 8 28.674203764434246 35.0 27.0 37.0 2.0 37.0 9 29.104523956390963 35.0 26.0 39.0 2.0 39.0 10-11 29.280996313095443 35.0 27.0 39.0 2.0 39.0 12-13 29.1405515643171 35.0 27.0 39.0 2.0 39.0 14-15 29.727157567774814 36.0 26.5 40.0 2.0 41.0 16-17 29.936899958405604 36.0 27.0 40.0 2.0 41.0 18-19 29.775922751851233 36.0 27.0 40.0 2.0 41.0 20-21 29.520083266603095 35.5 26.0 40.0 2.0 41.0 22-23 29.559386610000196 36.0 26.0 40.0 2.0 41.0 24-25 29.615128935551326 36.0 26.0 40.0 2.0 41.0 26-27 29.71004922003435 36.0 26.5 40.0 2.0 41.0 28-29 29.658303988534662 36.0 26.5 40.0 2.0 41.0 30-31 29.273130515855385 35.0 25.0 40.0 2.0 41.0 32-33 29.33818471589896 35.0 25.0 40.0 2.0 41.0 34-35 29.015983424492024 35.0 25.0 40.0 2.0 41.0 36-37 28.761862360750737 35.0 24.0 39.0 2.0 41.0 38-39 28.611336807078402 35.0 24.0 39.0 2.0 41.0 40-41 28.70759546904047 35.0 24.0 39.0 2.0 41.0 42-43 28.54042225385031 35.0 24.0 39.0 2.0 40.5 44-45 28.15833918107144 34.0 23.5 39.0 2.0 40.0 46-47 27.883844821780748 34.0 22.5 39.0 2.0 40.0 48-49 27.65383807330234 33.5 21.5 39.0 2.0 40.0 50-51 27.623631381697898 33.5 21.5 39.0 2.0 40.0 52-53 27.496660776485303 33.0 20.5 39.0 2.0 40.0 54-55 27.369272812545095 33.0 21.0 38.5 2.0 40.0 56-57 26.99554876306191 33.0 18.5 38.0 2.0 40.0 58-59 26.673063986044653 33.0 16.5 38.0 2.0 40.0 60-61 26.31999848619038 32.5 14.0 38.0 2.0 40.0 62-63 26.220763844284164 32.0 14.0 38.0 2.0 40.0 64-65 26.00342481742324 32.0 13.5 37.0 2.0 40.0 66-67 25.795957916092505 32.0 13.0 37.0 2.0 40.0 68-69 25.600725815122818 32.0 12.0 36.0 2.0 39.0 70-71 25.55175089060108 32.0 12.0 36.0 2.0 39.0 72-73 25.555234951742012 32.0 12.0 36.0 2.0 39.0 74-75 25.262969776154243 32.0 9.0 35.5 2.0 39.0 76-77 24.982352799698937 32.0 8.0 35.0 2.0 38.0 78-79 24.756893492882266 32.0 7.0 35.0 2.0 37.0 80-81 24.700312736090638 32.0 7.0 35.0 2.0 37.0 82-83 24.493614234620686 32.0 4.5 35.0 2.0 36.0 84-85 24.30173745023528 31.0 2.0 35.0 2.0 36.0 86-87 24.082749325859545 31.0 2.0 35.0 2.0 36.0 88-89 23.881598512222244 31.0 2.0 35.0 2.0 35.0 90-91 23.739568684513586 31.0 2.0 34.5 2.0 35.0 92-93 23.620622861920758 31.0 2.0 34.0 2.0 35.0 94-95 23.566690210886414 31.0 2.0 34.0 2.0 35.0 96-97 23.477302582247965 31.0 2.0 34.0 2.0 35.0 98-99 23.36361565646991 31.0 2.0 34.0 2.0 35.0 100-101 23.26591816797911 31.0 2.0 34.0 2.0 35.0 102-103 22.12865949328406 29.0 2.0 33.5 2.0 34.5 104-105 22.958528867076197 30.5 2.0 34.0 2.0 35.0 106-107 23.039586298466666 31.0 2.0 34.0 2.0 35.0 108-109 22.97658529114662 31.0 2.0 34.0 2.0 35.0 110-111 22.849024017226306 31.0 2.0 34.0 2.0 35.0 112-113 22.78915673730168 31.0 2.0 34.0 2.0 35.0 114-115 22.727226714919937 31.0 2.0 34.0 2.0 35.0 116-117 22.566451467546468 31.0 2.0 34.0 2.0 35.0 118-119 22.38939197907264 31.0 2.0 34.0 2.0 35.0 120-121 22.19957210119605 31.0 2.0 34.0 2.0 35.0 122-123 21.918082208351137 31.0 2.0 34.0 2.0 35.0 124-125 20.830885889879546 28.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.3841265232078968 1101 2 1.9763308196538247 1101 3 2.3272964627788824 1101 4 2.80441134446826 1101 5 2.339260620456976 1101 6 2.1359262981826 1101 7 2.086777907901187 1101 8 2.3236829732678466 1101 9 2.9916370855519574 1101 10-11 3.039804924548008 1101 12-13 2.7765623390155696 1101 14-15 3.2148734267206187 1101 16-17 2.924366855021674 1101 18-19 2.878844470725401 1101 20-21 3.176810932215833 1101 22-23 2.958624216317272 1101 24-25 2.894382949409671 1101 26-27 2.6125055331633504 1101 28-29 2.523909568386223 1101 30-31 2.623659335622378 1101 32-33 3.0045900331639146 1101 34-35 3.165059478026091 1101 36-37 3.263290587722164 1101 38-39 3.297133732388314 1101 40-41 3.084877227805695 1101 42-43 2.9754057706865176 1101 44-45 3.2235656281759653 1101 46-47 3.235563018237851 1101 48-49 3.279172098990042 1101 50-51 3.144108649022094 1101 52-53 3.069838787790463 1101 54-55 3.135440517903298 1101 56-57 3.3225895925213713 1101 58-59 3.2075092777076435 1101 60-61 3.487947159957976 1101 62-63 3.1816807599138492 1101 64-65 3.0982246135305616 1101 66-67 3.256407321017388 1101 68-69 3.0451695067191054 1101 70-71 3.0701380525639017 1101 72-73 2.8766676632838823 1101 74-75 2.956866824866381 1101 76-77 3.2214101585594506 1101 78-79 3.100612250441099 1101 80-81 2.9433736450096966 1101 82-83 2.9132244070207634 1101 84-85 2.7919296371307354 1101 86-87 2.791184160543395 1101 88-89 2.930498309699054 1101 90-91 2.932288998206392 1101 92-93 2.968838240107729 1101 94-95 2.9159726684520137 1101 96-97 2.935208758369427 1101 98-99 2.8392611992201076 1101 100-101 2.824409316958654 1101 102-103 2.728945448730535 1101 104-105 2.757990547951767 1101 106-107 2.940621251384826 1101 108-109 3.0432004480692356 1101 110-111 3.1251157907840366 1101 112-113 3.069465782336575 1101 114-115 2.966203849507785 1101 116-117 2.9962122749982285 1101 118-119 3.0236910059336104 1101 120-121 3.0356256115986753 1101 122-123 3.051870930586542 1101 124-125 3.056812427677187 1102 1 1.3909472042218702 1102 2 1.1699715213550448 1102 3 1.291543176717557 1102 4 1.9692735922264433 1102 5 1.766709990915544 1102 6 1.6360477698517357 1102 7 1.4962435194026682 1102 8 1.7071454870733156 1102 9 2.1493683661126575 1102 10-11 1.8501646835781607 1102 12-13 2.1390403880181026 1102 14-15 2.4261090283037277 1102 16-17 1.9927455784558745 1102 18-19 2.121890844678166 1102 20-21 2.143747279713601 1102 22-23 2.014833237877763 1102 24-25 1.8300519464856677 1102 26-27 1.8315824772490998 1102 28-29 1.652191905080386 1102 30-31 1.524124888428151 1102 32-33 1.5502452442348869 1102 34-35 1.9615089154157346 1102 36-37 2.1458240585012796 1102 38-39 2.215178893496063 1102 40-41 1.7364493145220194 1102 42-43 1.7984163829306858 1102 44-45 2.167271763614515 1102 46-47 2.1615991060669977 1102 48-49 2.293135314149346 1102 50-51 2.188436880370638 1102 52-53 2.27460741738939 1102 54-55 2.1566453910846306 1102 56-57 2.4138899637717373 1102 58-59 2.4180691861123904 1102 60-61 2.717746683414699 1102 62-63 2.5546095088627148 1102 64-65 2.6855647463326022 1102 66-67 2.536253752378144 1102 68-69 2.3215019629608555 1102 70-71 2.571547217509629 1102 72-73 2.566927596933777 1102 74-75 2.59655508925821 1102 76-77 2.4228607960667254 1102 78-79 2.3482721146443843 1102 80-81 2.004922909276946 1102 82-83 1.9070352663858472 1102 84-85 1.9419840017856203 1102 86-87 2.0843990076530474 1102 88-89 1.7326518245838045 1102 90-91 1.6223387428201583 1102 92-93 1.6084439262246377 1102 94-95 1.8522787460633126 1102 96-97 1.9890295233841506 1102 98-99 2.106240205921001 1102 100-101 1.9559956456198897 1102 102-103 1.6103463131745919 1102 104-105 1.6772303614012323 1102 106-107 1.99903574176831 1102 108-109 2.115098499611257 1102 110-111 1.870559049701594 1102 112-113 1.757666761836198 1102 114-115 1.9291038937092502 1102 116-117 2.0504666508232603 1102 118-119 2.141363250888773 1102 120-121 2.1408162775733715 1102 122-123 2.3034939717669722 1102 124-125 2.2001158499065667 1103 1 2.0326063827930447 1103 2 1.7852362252967247 1103 3 1.862634348766651 1103 4 2.6965865529248987 1103 5 2.060503971897745 1103 6 2.007940930682107 1103 7 1.963374214074495 1103 8 2.2644719620859064 1103 9 3.0871231021763315 1103 10-11 2.481387442508044 1103 12-13 2.664766482523536 1103 14-15 3.0406845055928535 1103 16-17 2.767505874255015 1103 18-19 2.4774174221868144 1103 20-21 2.877280795860134 1103 22-23 2.7569133815893174 1103 24-25 2.7965789900658073 1103 26-27 2.533739310735527 1103 28-29 2.3513458840894685 1103 30-31 2.59804615449584 1103 32-33 2.680663521782421 1103 34-35 2.4717830727154038 1103 36-37 2.9327401072854506 1103 38-39 2.929059391688419 1103 40-41 2.918985541353507 1103 42-43 2.9445178556724265 1103 44-45 2.836973770910653 1103 46-47 2.9267524831840284 1103 48-49 2.993736667257462 1103 50-51 2.8659517136861723 1103 52-53 2.892898779025874 1103 54-55 2.8436087356642332 1103 56-57 2.8984880657196435 1103 58-59 3.1200121087664883 1103 60-61 3.057821997393546 1103 62-63 2.8270043786405914 1103 64-65 2.794605227034836 1103 66-67 2.8867404477082275 1103 68-69 2.841773359311855 1103 70-71 2.915971502824572 1103 72-73 2.8785685828854994 1103 74-75 2.926804558579324 1103 76-77 2.9274727630145385 1103 78-79 3.1035755257614674 1103 80-81 2.600235352143141 1103 82-83 2.7722708057721803 1103 84-85 2.7376104936737917 1103 86-87 2.7353965514679075 1103 88-89 2.7827863969719147 1103 90-91 2.761362987557355 1103 92-93 2.6847137764144478 1103 94-95 2.4622843798657073 1103 96-97 2.6586545070615415 1103 98-99 2.5515485898895065 1103 100-101 2.3830356028758963 1103 102-103 2.452587794794635 1103 104-105 2.7151528540082914 1103 106-107 2.4108131957574592 1103 108-109 2.7611739796886567 1103 110-111 2.9758843010192173 1103 112-113 2.810709200390498 1103 114-115 2.7378740282464555 1103 116-117 2.9731670307802496 1103 118-119 3.009988185050986 1103 120-121 2.8988592668577695 1103 122-123 2.9011366410852304 1103 124-125 2.849899672903149 1104 1 1.631697378490415 1104 2 1.5197606576385994 1104 3 1.4865928392523955 1104 4 1.9240982676961096 1104 5 1.5803241098814418 1104 6 1.3884493020518924 1104 7 1.3628541404967827 1104 8 1.738231576695 1104 9 2.2781674025214045 1104 10-11 1.987264599690537 1104 12-13 1.4401891051218385 1104 14-15 1.910563234807352 1104 16-17 1.56306674428712 1104 18-19 0.8182951761697908 1104 20-21 -3.647557170758457 1104 22-23 -0.358289320475226 1104 24-25 1.053463713623504 1104 26-27 1.2521633882154468 1104 28-29 0.9080709951479946 1104 30-31 1.600949199489893 1104 32-33 1.4506548322675457 1104 34-35 1.76517380938105 1104 36-37 1.9254105989638717 1104 38-39 1.9124429799352178 1104 40-41 1.9317544165455054 1104 42-43 1.9135485971523885 1104 44-45 2.0347450766078303 1104 46-47 1.9414940076280978 1104 48-49 2.007930272186517 1104 50-51 1.996083979571715 1104 52-53 2.0510071770262535 1104 54-55 2.0341472079469405 1104 56-57 2.3017211526094847 1104 58-59 2.28420804993155 1104 60-61 2.3457211894693195 1104 62-63 1.9235410183591064 1104 64-65 2.140740010994307 1104 66-67 2.013317571848397 1104 68-69 1.9553107046770002 1104 70-71 2.1056792837164977 1104 72-73 2.0038051067331217 1104 74-75 2.03277014701694 1104 76-77 2.0216129342522606 1104 78-79 1.945421882122531 1104 80-81 1.724931351883395 1104 82-83 1.7797233629847469 1104 84-85 1.6401640406128735 1104 86-87 1.56355427558983 1104 88-89 1.7386965982613702 1104 90-91 1.728166054053336 1104 92-93 1.4156573167361515 1104 94-95 1.5682775718850976 1104 96-97 1.6932687570747191 1104 98-99 1.5700186115621477 1104 100-101 1.0391555450541468 1104 102-103 1.4501979910908496 1104 104-105 1.6439963622998626 1104 106-107 1.7867633051613794 1104 108-109 1.8636142753153528 1104 110-111 1.8593278829512307 1104 112-113 1.8095690423754043 1104 114-115 1.668174414022996 1104 116-117 1.704245007138681 1104 118-119 1.7055496894843287 1104 120-121 1.778633995969333 1104 122-123 1.5892482613449097 1104 124-125 1.652561613351903 1105 1 1.663306773621315 1105 2 1.4809470788080894 1105 3 1.470818049124265 1105 4 2.086195835373058 1105 5 1.684206433119389 1105 6 1.6069236700229013 1105 7 1.457070253755191 1105 8 1.7157766420606642 1105 9 2.2529610579119854 1105 10-11 1.9703699050582841 1105 12-13 2.046941502729247 1105 14-15 2.3219786121682944 1105 16-17 2.0913878064745433 1105 18-19 1.7050728400851973 1105 20-21 1.9280003959531378 1105 22-23 1.778763228247346 1105 24-25 1.8676332299435714 1105 26-27 1.6833500878526948 1105 28-29 1.2385450957899593 1105 30-31 1.6854358534584293 1105 32-33 1.6666625142647895 1105 34-35 1.6222239447163815 1105 36-37 1.8048625372851212 1105 38-39 1.655416495794885 1105 40-41 1.872347107323769 1105 42-43 2.225070163552097 1105 44-45 2.299949546201006 1105 46-47 2.607837336097525 1105 48-49 2.2148409508681404 1105 50-51 2.244840621027766 1105 52-53 2.2478663391724574 1105 54-55 2.27367167783715 1105 56-57 2.560295341989839 1105 58-59 2.2364232423899004 1105 60-61 2.6291349257269374 1105 62-63 2.5399886237220244 1105 64-65 2.2810712205468633 1105 66-67 2.4824859000393573 1105 68-69 2.060163822216385 1105 70-71 2.1414945816213056 1105 72-73 2.092920173324046 1105 74-75 2.4204578064397317 1105 76-77 2.1910472362821025 1105 78-79 2.0619450079416914 1105 80-81 1.9992314881469504 1105 82-83 2.1401723458348485 1105 84-85 2.055104764109238 1105 86-87 2.1054406718828673 1105 88-89 2.192498147284688 1105 90-91 2.2544532434225495 1105 92-93 2.205954794560835 1105 94-95 2.0358797231413597 1105 96-97 2.125706853691895 1105 98-99 1.9765795904320171 1105 100-101 2.1327291403699498 1105 102-103 2.1392594990494587 1105 104-105 2.2175971360689424 1105 106-107 2.3171864090744094 1105 108-109 2.3627504126333747 1105 110-111 2.2342891016746584 1105 112-113 2.2882949224263456 1105 114-115 2.2111379978659222 1105 116-117 2.375849352720884 1105 118-119 2.399917382026217 1105 120-121 2.396232081596313 1105 122-123 2.5177476728497155 1105 124-125 2.103561999493863 1106 1 -0.330767370002615 1106 2 -0.04987150560891607 1106 3 -0.04961025627610738 1106 4 -0.5323932762492447 1106 5 -1.0200326479879145 1106 6 -0.35033123193788285 1106 7 -0.28250629194689125 1106 8 0.11207697224860524 1106 9 0.11251459999920854 1106 10-11 -2.3904375687692365 1106 12-13 -0.5219381273350692 1106 14-15 -0.16397306459179717 1106 16-17 -0.3157135033231171 1106 18-19 -3.2519271442660873 1106 20-21 -5.570478705184524 1106 22-23 -6.410709488213538 1106 24-25 -6.284042669466473 1106 26-27 -5.262597833215175 1106 28-29 -1.9124917882373964 1106 30-31 -3.794367520405082 1106 32-33 -4.338193986994931 1106 34-35 -3.6674899184825307 1106 36-37 -6.134572387522791 1106 38-39 -6.569847185096467 1106 40-41 -2.760043156044148 1106 42-43 -1.256687656894517 1106 44-45 -0.42642214213913476 1106 46-47 -0.25623430373159195 1106 48-49 -0.03077709523298111 1106 50-51 -0.0497582240968768 1106 52-53 -0.22771109850652138 1106 54-55 -0.1021537137720081 1106 56-57 -0.012720948325171122 1106 58-59 0.12814726160652512 1106 60-61 0.3368092133190679 1106 62-63 0.0978188507849147 1106 64-65 -0.18081765790954663 1106 66-67 -0.49268897437968207 1106 68-69 -0.2672567996636488 1106 70-71 -0.10063935575252358 1106 72-73 -0.03771297378954941 1106 74-75 -0.10244158795489611 1106 76-77 -0.1833554521675751 1106 78-79 -0.2617509844217345 1106 80-81 -0.6391034100581408 1106 82-83 -0.7724409900022806 1106 84-85 -0.8038838592730748 1106 86-87 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114-115 -7.777851867743241 2216 116-117 -7.412833176470569 2216 118-119 -7.650182860317912 2216 120-121 -8.260143772115958 2216 122-123 -11.412892335962802 2216 124-125 -9.680391514496003 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 306347.0 3 45441.0 4 26845.0 5 21633.0 6 21487.0 7 23712.0 8 25617.0 9 27535.0 10 28585.0 11 29055.0 12 29482.0 13 29303.0 14 28434.0 15 25012.0 16 21447.0 17 18996.0 18 17431.0 19 16548.0 20 16524.0 21 18546.0 22 23348.0 23 32488.0 24 44216.0 25 56170.0 26 65400.0 27 73349.0 28 82886.0 29 93272.0 30 104847.0 31 118687.0 32 139216.0 33 165295.0 34 201369.0 35 246077.0 36 296307.0 37 253792.0 38 52550.0 39 55.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19313482158153 18.747424920960118 13.67566959134292 23.383770666115428 2 15.17419905370209 20.257065570789436 40.20393448793848 24.364800887569988 3 18.929033020461723 25.47927592671806 28.86828788150576 26.72340317131445 4 11.576747291520785 16.671189791866652 37.66101470623098 34.09104821038158 5 13.589778792437981 38.86312136339382 33.51342687015856 14.033672974009633 6 31.671214755260955 36.86774347011791 17.64731474128951 13.813727033331624 7 28.766147453369484 32.76381159482828 20.519724988380837 17.95031596342139 8 25.661302861961317 36.23465012651996 19.678893274033662 18.425153737485065 9 26.371161603513432 16.942427059324565 19.999207666393595 36.68720367076841 10-11 24.38286830202182 27.5945620069557 27.21327707123928 20.8092926197832 12-13 25.32152216813065 24.82074380840572 27.46928919008496 22.388444833378667 14-15 22.450691580692197 26.192199368801294 25.445631096122323 25.91147795438419 16-17 22.37180879553014 29.428135592980496 25.647774797523944 22.552280813965417 18-19 22.217558531238033 28.037791816018277 27.774738419114886 21.969911233628807 20-21 23.18635688344527 27.120970926392545 27.858143319303252 21.834528870858925 22-23 22.891761253290753 27.112165362157757 27.343641111592998 22.652432272958496 24-25 22.52557143286692 27.163945473551276 27.6573330715611 22.653150022020704 26-27 22.486591742547308 27.49413927609117 27.326510634196204 22.69275834716532 28-29 22.422525845351462 27.513213383430855 27.39570999442018 22.668550776797503 30-31 22.533959106176233 27.131384000701747 27.702527284714616 22.6321296084074 32-33 22.521658144424418 27.20571381678547 27.37447260667423 22.898155432115882 34-35 22.65943300210876 27.15112903961853 27.574189458476294 22.61524849979641 36-37 22.74206005114943 26.9146063437531 27.562861078594718 22.780472526502752 38-39 22.589196224530998 27.148524100919 27.5481647851047 22.7141148894453 40-41 22.809930309089115 27.17883606944253 27.375521744222294 22.635711877246063 42-43 22.6926485775751 27.10820326960604 27.201626649257822 22.997521503561035 44-45 22.69027434741231 27.1747258225324 27.358885700851985 22.776114129203297 46-47 22.775651297944613 27.36532833141417 27.007639941050833 22.85138042959039 48-49 22.826636387593364 27.237034560944593 27.035233026828458 22.90109602463358 50-51 22.837108858216187 27.385142448832323 26.939472511301815 22.838276181649675 52-53 23.038399838696268 27.207765218175677 26.920602129845967 22.833232813282088 54-55 22.81551045587901 27.205341875655726 26.881096079195704 23.098051589269556 56-57 22.77565418918601 27.440405941551898 26.905247467684717 22.87869240157737 58-59 22.91032508628676 27.090559271232806 26.932736828290665 23.066378814189765 60-61 22.818584652375833 26.97834262443301 27.314170193321978 22.888902529869178 62-63 22.73925184603116 27.18684677485963 27.08700872649981 22.9868926526094 64-65 22.66599648650623 27.05620122100364 27.098134643603935 23.1796676488862 66-67 22.744036865185798 27.321782454018003 26.7320526731416 23.2021280076546 68-69 22.658365978971197 27.171617307641288 27.04555061326636 23.12446610012115 70-71 22.497488207707757 26.983095606923474 27.310632260697954 23.208783924670815 72-73 22.609627261034348 27.122667828209757 27.021421875174084 23.246283035581808 74-75 22.581164300829883 27.301619695967013 26.96682546593715 23.150390537265952 76-77 22.706354680674202 27.026387930882013 27.150775543410976 23.11648184503281 78-79 22.736156443971534 27.078129659429013 27.09919492141472 23.086518975184735 80-81 22.600078558527077 27.31201618284435 26.918374648480658 23.169530610147916 82-83 22.72443659832121 27.34489099827141 26.8649565623699 23.06571584103748 84-85 22.819825407411297 27.16529243224019 26.821260744468795 23.19362141587972 86-87 22.611841827181813 27.14394783341029 26.98621078614383 23.257999553264064 88-89 22.69378214846356 27.094005273214634 26.936159014232867 23.27605356408894 90-91 22.720226602722423 27.293913076081378 26.95006862917273 23.03579169202347 92-93 22.58173879733603 27.167855059781616 26.974660857871392 23.275745285010967 94-95 22.48895276100506 27.03498931973484 27.009592961459433 23.46646495780066 96-97 22.658031633296403 26.910072270065267 27.01047044956349 23.421425647074845 98-99 22.49175024595842 27.022213277386452 26.8001211797303 23.685915296924826 100-101 22.414095277103822 27.04837072081321 26.99131813923695 23.54621586284602 102-103 22.403400854422586 26.667873947821757 27.32012409765608 23.608601100099584 104-105 22.31612879950256 26.773932383019993 27.27747324458366 23.63246557289379 106-107 22.26652464582181 26.897699895429696 27.19208130628524 23.643694152463258 108-109 22.28877427136176 26.84552542154069 27.02686595143208 23.83883435566548 110-111 22.148279239905442 26.99651799882819 27.186065911670106 23.66913684959626 112-113 22.20305731803702 27.009533935586465 27.16588913512637 23.621519611250147 114-115 22.1392656929409 27.12520521262619 27.02454204181766 23.710987052615256 116-117 21.893298259743702 27.051903147232142 27.33428515896567 23.720513434058486 118-119 21.974710577970413 27.059617905090477 27.08835628660496 23.877315230334148 120-121 21.96510057998276 27.16646393032142 27.21593065532711 23.65250483436871 122-123 21.966805018879956 27.11123302187726 27.105856686348652 23.816105272894124 124-125 22.020538874298698 27.093472030862987 26.95599416754146 23.929994927296857 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1494.0 1 1505.5 2 1321.0 3 996.5 4 773.0 5 639.5 6 621.5 7 705.0 8 885.5 9 1002.0 10 1017.5 11 1065.0 12 1143.5 13 1264.5 14 1400.0 15 1516.5 16 1730.0 17 1961.0 18 2181.5 19 2568.5 20 3112.0 21 3933.0 22 5039.5 23 6450.0 24 8171.0 25 10581.0 26 14183.5 27 18229.0 28 22246.0 29 26663.0 30 32261.5 31 38694.5 32 44940.5 33 50849.0 34 56794.5 35 61940.0 36 67420.0 37 73761.0 38 78180.5 39 80912.5 40 83584.0 41 86419.5 42 88695.5 43 91554.5 44 95841.5 45 99794.0 46 101698.0 47 102583.5 48 103985.5 49 103816.5 50 101333.0 51 98457.5 52 97071.5 53 96631.5 54 95092.0 55 89806.0 56 82726.5 57 76458.5 58 70868.0 59 64754.5 60 56365.5 61 46769.5 62 37738.0 63 29852.0 64 22929.5 65 17419.5 66 13159.0 67 10232.0 68 7863.0 69 5862.0 70 4496.5 71 3418.5 72 2683.0 73 2231.0 74 1705.5 75 1223.5 76 841.5 77 548.0 78 372.0 79 251.5 80 178.0 81 130.0 82 90.0 83 63.0 84 52.5 85 46.5 86 39.5 87 33.0 88 19.5 89 18.0 90 17.5 91 13.0 92 12.5 93 10.0 94 11.0 95 5.5 96 2.5 97 2.5 98 2.0 99 4.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07597343617806929 2 0.025112262423849716 3 0.0320092922444845 4 0.03844652007707696 5 0.04173587276076432 6 0.003855262822816365 7 0.0027588119282539124 8 0.0029002894630361644 9 0.007498309343459351 10-11 0.01469597892550642 12-13 0.007250723657590411 14-15 0.010681553876060021 16-17 0.01945316103255964 18-19 0.017737745923324836 20-21 0.1276481057572868 22-23 0.015633267593438836 24-25 0.01481977176844089 26-27 0.0078343184885672 28-29 0.0053584616298777915 30-31 0.0028295506956450387 32-33 0.006790921669548092 34-35 0.018427448905388315 36-37 0.0034485149103173907 38-39 0.009355201987476408 40-41 0.0342021940336094 42-43 0.01374100556572622 44-45 0.10101495983452788 46-47 0.004332749502706465 48-49 0.009514364214106442 50-51 0.011459680317362405 52-53 0.011990221072795852 54-55 0.00997416620214876 56-57 0.006985453279873689 58-59 0.013900167792356252 60-61 0.030187768984163005 62-63 0.007975796023349453 64-65 0.007197669582047066 66-67 0.008718553080956275 68-69 0.008099588866283923 70-71 0.004934029025531036 72-73 0.0010787662027146709 74-75 0.0015916222663003342 76-77 0.008983823458672997 78-79 0.01326351888583612 80-81 0.003979055665750835 82-83 0.005199299403247758 84-85 0.003307037375535139 86-87 0.0046157045722709684 88-89 0.006578705367374714 90-91 0.005429200397268918 92-93 0.0034308302184696093 94-95 0.004491911729336499 96-97 0.0021752170972771235 98-99 0.0034838842940129538 100-101 0.0030948210733617613 102-103 5.128560635856632E-4 104-105 9.196039760846376E-4 106-107 6.897029820634781E-4 108-109 9.372886679324189E-4 110-111 0.0017861538766259304 112-113 0.0030948210733617613 114-115 0.0018922620277126193 116-117 0.0037314699798818947 118-119 0.002635019085319442 120-121 0.005340776938030011 122-123 0.003519253677708517 124-125 0.049322605563462574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2827304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.63280905174037 #Duplication Level Percentage of deduplicated Percentage of total 1 80.17643069298344 49.414986433507444 2 10.269195374007355 12.658387152024217 3 3.911056157390315 7.231481320172121 4 1.8471323916096898 4.553758319414581 5 1.0689819029986467 3.294217875364082 6 0.6405399290873024 2.3686965083671816 7 0.45204554162340277 1.9502585584696017 8 0.2958090217382007 1.4585232762058113 9 0.21377642463149496 1.1858077403169027 >10 1.0514951740431278 11.332870463116144 >50 0.055032183348315195 2.3224585752206597 >100 0.01790902995751716 1.8855114448036046 >500 4.1732360685400193E-4 0.1534798441770666 >1k 1.788529743075855E-4 0.1895624888405867 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.536938369556298E-5 0.0 0.0 0.0 0.0 2 3.536938369556298E-5 0.0 0.0 0.0 0.0 3 3.536938369556298E-5 0.0 0.0 0.0 0.0 4 3.536938369556298E-5 0.0 0.0 0.0 0.0 5 3.536938369556298E-5 0.0 0.0 0.0 0.0 6 3.536938369556298E-5 0.0 0.0 0.0 0.0 7 7.073876739112596E-5 0.0 0.0 3.536938369556298E-5 0.0 8 7.073876739112596E-5 0.0 0.0 3.536938369556298E-5 0.0 9 7.073876739112596E-5 0.0 0.0 7.073876739112596E-5 0.0 10-11 7.073876739112596E-5 0.0 0.0 1.4147753478225194E-4 0.0 12-13 1.0610815108668894E-4 0.0 0.0 1.7684691847781492E-4 0.0 14-15 2.122163021733779E-4 0.0 0.0 1.7684691847781492E-4 0.0 16-17 2.6527037771672233E-4 0.0 0.0 1.7684691847781492E-4 0.0 18-19 3.0063976141228533E-4 0.0 0.0 1.7684691847781492E-4 0.0 20-21 3.1832445326006683E-4 0.0 0.0 2.475856858689409E-4 0.0 22-23 3.1832445326006683E-4 0.0 0.0 4.774866798901002E-4 0.0 24-25 3.890632206511928E-4 0.0 0.0 8.134958249979486E-4 0.0 26-27 4.244326043467558E-4 0.0 0.0 0.0010610815108668893 0.0 28-29 5.659101391290078E-4 0.0 0.0 0.0016800457255392418 0.0 30-31 6.366489065201337E-4 0.0 0.0 0.003307037375535139 0.0 32-33 6.543335983679151E-4 0.0 0.0 0.007303777733133756 0.0 34-35 7.073876739112597E-4 0.0 0.0 0.012290860834208137 0.0 36-37 7.781264413023856E-4 0.0 0.0 0.02219428826896577 0.0 38-39 8.488652086935116E-4 0.0 0.0 0.0407985840928319 0.0 40-41 8.665499005412931E-4 0.0 0.0 0.06288676421071099 0.0 42-43 9.196039760846376E-4 0.0 0.0 0.0853286381655457 0.0 44-45 9.903427434757636E-4 0.0 0.0 0.1104585852812432 0.0 46-47 0.0011141355864102338 0.0 0.0 0.14342285088550788 0.0 48-49 0.0011671896619535784 0.0 0.0 0.1828773983979084 0.0 50-51 0.0011671896619535784 0.0 0.0 0.22411809978693484 0.0 52-53 0.00118487435380136 0.0 0.0 0.2650051073390056 0.0 54-55 0.0012379284293447044 0.0 0.0 0.3112505765209542 0.0 56-57 0.0013440365804313933 0.0 0.0 0.36713420275994374 0.0 58-59 0.0013440365804313933 0.0 0.0 0.4328858870499953 0.0 60-61 0.0013440365804313933 0.0 0.0 0.5091953323731724 0.0 62-63 0.0013440365804313933 0.0 0.0 0.5875915713343878 0.0 64-65 0.0013794059641269562 0.0 0.0 0.6672434234167957 0.0 66-67 0.0014147753478225193 0.0 0.0 0.7632713001502491 0.0 68-69 0.0014501447315180822 0.0 0.0 0.8754629852325749 0.0 70-71 0.0015208834989092082 0.0 0.0 1.0056930559996378 0.0 72-73 0.0016093069581481156 0.0 0.0 1.1395661732873437 0.0 74-75 0.0016446763418436787 0.0 0.0 1.2778958329206906 0.0 76-77 0.0016800457255392418 0.0 0.0 1.4367043657137684 0.0 78-79 0.001697730417387023 0.0 0.0 1.6160625104339683 0.0 80-81 0.001980685486951527 0.0 0.0 1.820762818572039 0.0 82-83 0.0019983701787993084 0.0 0.0 2.035366554144867 0.0 84-85 0.002051424254342653 0.0 0.0 2.254409147371489 0.0 86-87 0.0021398477135815604 0.0 0.0 2.4895271254877436 0.0 88-89 0.002192901789124905 0.0 0.0 2.754408439983815 0.0 90-91 0.0024228027831460644 0.0 0.0 3.054340813722189 0.0 92-93 0.0025112262423849716 0.0 0.0 3.3721347262268226 0.0 94-95 0.0025112262423849716 0.0 0.0 3.697603794993393 0.0 96-97 0.0025112262423849716 0.0 0.0 4.028961866145275 0.0 98-99 0.0026527037771672235 0.0 0.0 4.390366228746537 0.0 100-101 0.0027764966201016938 0.0 0.0 4.804453288362341 0.0 102-103 0.0029002894630361644 0.0 0.0 5.256155687538376 0.0 104-105 0.003307037375535139 0.0 0.0 5.704073562658985 0.0 106-107 0.0035723077532518613 0.0 0.0 6.146491498614935 0.0 108-109 0.0035723077532518613 0.0 0.0 6.617682428207225 0.0 110-111 0.0036253618287952058 0.0 0.0 7.138567341891781 0.0 112-113 0.00367841590433855 0.0 0.0 7.6991367040827585 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7170 0.0 45.656487 1 GGTATCA 4455 0.0 41.41646 1 ATCAACG 8060 0.0 38.312244 3 TCAACGC 8150 0.0 37.968887 4 CAACGCA 8275 0.0 37.682327 5 AACGCAG 8415 0.0 36.61815 6 GTACATG 11640 0.0 36.100292 1 TATCAAC 8805 0.0 36.083572 2 TACATGG 12115 0.0 33.542458 2 ACATGGG 12205 0.0 31.345736 3 ACGCAGA 9955 0.0 30.297222 7 CGCAGAG 9980 0.0 29.387335 8 GCAGAGT 10585 0.0 26.302607 9 CATGGGA 7995 0.0 25.902573 4 AGTACAT 8575 0.0 22.896938 2 CATGGGG 5805 0.0 22.450396 4 GAGTACA 9605 0.0 22.370146 1 GTATAGG 1095 0.0 22.285824 1 CAGAGTA 10610 0.0 21.194357 10-11 AGAGTAC 10250 0.0 21.155218 10-11 >>END_MODULE