FastQCFastQC Report
Thu 26 May 2016
SRR1364506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827304
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61910.21897185445923043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60930.21550565485706522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36830.13026544015075847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56250.062.4427571
GTATCAA95500.047.2695851
ATCAACG123200.035.3009033
TATCAAC126450.035.288782
TCAACGC123550.034.766924
CAACGCA124200.034.1025055
AACGCAG129750.032.6437846
GTACATG116000.029.7138651
TACATGG119750.028.0593262
CGCAGAG155050.027.1574828
ACGCAGA154750.027.0626227
ACATGGG119350.026.0069223
GCAGAGT165700.024.2993249
GAGTACT97150.023.75375612-13
GTACTTT102800.022.53500614-15
AGAGTAC158650.022.21220410-11
CAGAGTA165550.022.11272210-11
AGTACTT102100.022.07785412-13
GTGGTAT32200.021.6680221
TGGTATC31300.020.9373632
Produced by FastQC (version 0.11.5)