##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364506_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2827304 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.46965271509537 31.0 28.0 31.0 23.0 34.0 2 28.65703086756854 31.0 28.0 31.0 23.0 34.0 3 28.740090913463852 31.0 28.0 33.0 23.0 34.0 4 32.131526004985666 35.0 32.0 37.0 25.0 37.0 5 31.333386151612984 35.0 32.0 37.0 18.0 37.0 6 31.409393542399403 35.0 32.0 37.0 18.0 37.0 7 30.99707106133617 35.0 31.0 37.0 17.0 37.0 8 31.0177614434104 35.0 31.0 37.0 17.0 37.0 9 31.549108974485943 37.0 30.0 39.0 10.0 39.0 10-11 31.697928839629554 37.0 30.0 39.0 11.0 39.0 12-13 31.46099499735437 37.0 30.0 39.0 10.0 39.0 14-15 32.326579490567696 37.0 30.5 40.0 10.0 41.0 16-17 32.385776520671286 37.0 31.0 40.0 10.0 41.0 18-19 32.290758439842335 37.0 30.0 40.0 10.0 41.0 20-21 32.22306303107129 37.0 30.0 40.0 10.0 41.0 22-23 32.142028589780224 37.0 30.0 40.0 10.0 41.0 24-25 31.998620417188956 37.0 30.0 40.0 8.0 41.0 26-27 31.999416228322104 37.0 30.0 40.0 8.0 41.0 28-29 31.848438300232306 37.0 30.0 40.0 8.0 41.0 30-31 31.38573867543073 37.0 29.5 40.0 4.0 41.0 32-33 31.25858344203524 36.0 30.0 40.0 2.0 41.0 34-35 31.1143371918973 36.0 29.0 40.0 2.0 41.0 36-37 31.018095330392487 36.0 29.0 40.0 2.0 41.0 38-39 30.811337585204846 36.0 29.0 40.0 2.0 41.0 40-41 30.534842910419254 36.0 28.0 40.0 2.0 41.0 42-43 30.390108739633234 35.5 27.5 40.0 2.0 41.0 44-45 30.259639748679305 35.0 27.5 40.0 2.0 41.0 46-47 30.07201843169323 35.0 27.0 40.0 2.0 41.0 48-49 29.798513000370672 35.0 27.0 40.0 2.0 41.0 50-51 29.489428444907233 35.0 26.0 40.0 2.0 41.0 52-53 29.330945664137992 35.0 26.0 39.5 2.0 41.0 54-55 28.88817810182421 35.0 24.0 39.0 2.0 41.0 56-57 28.85254114874099 35.0 25.0 39.0 2.0 41.0 58-59 28.63881032955777 34.0 24.5 39.0 2.0 41.0 60-61 28.369663290541094 34.0 24.0 39.0 2.0 40.0 62-63 28.1203830574993 34.0 23.0 38.5 2.0 40.0 64-65 27.683035676389945 34.0 22.0 38.0 2.0 40.0 66-67 27.32738962630124 33.5 22.0 37.5 2.0 40.0 68-69 27.171159698426486 33.0 22.0 37.0 2.0 40.0 70-71 27.183950505499233 33.0 22.5 37.0 2.0 39.5 72-73 27.00339386921251 33.5 22.0 36.5 2.0 39.0 74-75 26.64106300560534 33.0 21.0 36.0 2.0 39.0 76-77 26.5105374236375 33.0 21.5 35.5 2.0 39.0 78-79 26.17002752445439 33.0 20.0 35.0 2.0 37.5 80-81 25.926764330966883 33.0 20.0 35.0 2.0 37.0 82-83 25.766215978189823 33.0 20.0 35.0 2.0 36.5 84-85 25.430007703451768 32.5 18.0 35.0 2.0 36.0 86-87 25.27688303061857 32.0 18.0 35.0 2.0 36.0 88-89 25.180859575058076 32.0 18.0 35.0 2.0 35.5 90-91 25.03272640649891 32.0 18.0 35.0 2.0 35.0 92-93 25.02776797259863 32.0 18.0 35.0 2.0 35.0 94-95 24.995211869682215 32.0 18.0 35.0 2.0 35.0 96-97 24.883985768774778 32.0 17.0 35.0 2.0 35.0 98-99 24.76577898945426 32.0 15.5 35.0 2.0 35.0 100-101 24.693143715709382 32.0 13.0 35.0 2.0 35.0 102-103 23.549441623539597 30.0 10.0 34.0 2.0 35.0 104-105 24.45477953555755 32.0 10.5 34.5 2.0 35.0 106-107 24.61504687858115 32.0 10.0 35.0 2.0 35.0 108-109 24.663781644987594 32.0 10.0 35.0 2.0 35.0 110-111 24.601656560454764 32.0 7.0 35.0 2.0 35.0 112-113 24.520658726475823 32.0 6.0 35.0 2.0 35.0 114-115 24.454874856046608 32.0 2.0 35.0 2.0 35.0 116-117 24.359514045889654 32.0 2.0 35.0 2.0 35.0 118-119 24.23308989765515 32.0 2.0 35.0 2.0 35.0 120-121 24.058225433133472 32.0 2.0 35.0 2.0 35.0 122-123 23.73540429327727 32.0 2.0 35.0 2.0 35.0 124-125 22.95613895781989 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4435733300293023 1101 2 1.4769106901348223 1101 3 1.4827484459641411 1101 4 1.5719354043580438 1101 5 1.9026049934174232 1101 6 1.9337957398359151 1101 7 2.3966935732980907 1101 8 2.2353422076393628 1101 9 3.017146920200542 1101 10-11 2.6875192315930008 1101 12-13 2.9067300938297223 1101 14-15 2.885331936259483 1101 16-17 2.8356555541900406 1101 18-19 2.890985775242058 1101 20-21 3.122496758089447 1101 22-23 3.1208262112840544 1101 24-25 3.25557145493633 1101 26-27 3.0326818052609354 1101 28-29 3.134275257486564 1101 30-31 3.638243636012163 1101 32-33 3.3457420708555006 1101 34-35 3.4160147625427584 1101 36-37 3.2853482533856955 1101 38-39 3.496022538166919 1101 40-41 3.64892153468681 1101 42-43 3.5667301761893384 1101 44-45 3.5296794278832557 1101 46-47 3.4387111009754427 1101 48-49 3.4420569629188122 1101 50-51 3.530084096970352 1101 52-53 3.449263868100367 1101 54-55 3.843549426904346 1101 56-57 3.5301835376814488 1101 58-59 3.7549898122281284 1101 60-61 3.8060662371706897 1101 62-63 3.7891373056121793 1101 64-65 3.8065852193856955 1101 66-67 4.062678563457016 1101 68-69 3.955136148663488 1101 70-71 3.6155261458692856 1101 72-73 3.6956586422120417 1101 74-75 3.8395629096658688 1101 76-77 3.6158824460405796 1101 78-79 3.6495397171050143 1101 80-81 3.721528025445423 1101 82-83 3.5540419330649797 1101 84-85 3.816481498256792 1101 86-87 3.7260900973816007 1101 88-89 3.5361501422091735 1101 90-91 3.488141633381396 1101 92-93 3.431039176227145 1101 94-95 3.400787784830385 1101 96-97 3.2594416810556055 1101 98-99 3.3970120146052913 1101 100-101 3.404239006990025 1101 102-103 3.1560631506762853 1101 104-105 3.275759225237678 1101 106-107 3.303151804967694 1101 108-109 3.329153261061908 1101 110-111 3.3283918418540495 1101 112-113 3.225876831518125 1101 114-115 3.2396533554407974 1101 116-117 3.3078171658763402 1101 118-119 3.1944368366508478 1101 120-121 3.0729795950991488 1101 122-123 3.252314342628992 1101 124-125 3.090785781442648 1102 1 0.8142264785150708 1102 2 -1.44606747476179 1102 3 0.2830528401166106 1102 4 0.8688302066732874 1102 5 1.3733376955522694 1102 6 0.8717018931826495 1102 7 1.7122466860273953 1102 8 1.4642016077572322 1102 9 2.254612953053371 1102 10-11 2.031937962142564 1102 12-13 2.0688346913808005 1102 14-15 2.2066871273559414 1102 16-17 2.105289343872535 1102 18-19 2.132214996661446 1102 20-21 2.3860519856610907 1102 22-23 2.376688557567178 1102 24-25 2.5007243610028382 1102 26-27 2.195650854354497 1102 28-29 2.284983173411547 1102 30-31 2.6908579384196543 1102 32-33 2.3916294257034068 1102 34-35 2.640316934942838 1102 36-37 2.07491303593007 1102 38-39 2.0075195602580607 1102 40-41 2.2441572323991146 1102 42-43 2.113025855526292 1102 44-45 2.098267928585212 1102 46-47 1.9177374544505916 1102 48-49 2.0102454119326048 1102 50-51 2.197969791418462 1102 52-53 2.0830643501538546 1102 54-55 2.329502323298037 1102 56-57 1.9242169129303086 1102 58-59 1.9295776171164043 1102 60-61 2.0083576869726834 1102 62-63 2.3564995788251366 1102 64-65 2.387599261136316 1102 66-67 2.5885938421897663 1102 68-69 2.5431328648481717 1102 70-71 1.900660562787305 1102 72-73 2.198253475462323 1102 74-75 2.418392145878965 1102 76-77 2.367973761441373 1102 78-79 2.2127957986992364 1102 80-81 2.3863402433466554 1102 82-83 1.970099132632786 1102 84-85 1.902963000423739 1102 86-87 2.0553506983957206 1102 88-89 2.156002738116765 1102 90-91 1.9636700707039623 1102 92-93 1.9108995852523876 1102 94-95 1.9747454830421987 1102 96-97 2.1039229629639102 1102 98-99 1.850238028873143 1102 100-101 1.6812202230153872 1102 102-103 1.3254424413026982 1102 104-105 1.8026367317840446 1102 106-107 1.9927983347635525 1102 108-109 1.9110816344780197 1102 110-111 1.850366946488137 1102 112-113 1.8372018355010518 1102 114-115 2.1145269612679627 1102 116-117 1.6928144901306084 1102 118-119 1.2438285836447847 1102 120-121 1.6267807262493221 1102 122-123 1.9605394869425723 1102 124-125 1.9047857600306912 1103 1 1.154922042212874 1103 2 1.0015034499374416 1103 3 0.9768344030051779 1103 4 0.9744615875776503 1103 5 1.448473674238958 1103 6 1.426743745863412 1103 7 1.6378309418009032 1103 8 1.5888561008746436 1103 9 2.44251252009305 1103 10-11 2.126934731679171 1103 12-13 2.1140828674311933 1103 14-15 1.7647429314542649 1103 16-17 1.860836812821404 1103 18-19 2.038905411154431 1103 20-21 2.296094274921309 1103 22-23 2.0563402536194033 1103 24-25 2.4854673407098034 1103 26-27 2.0918013650925786 1103 28-29 2.1793800307580824 1103 30-31 2.7629729669440337 1103 32-33 2.563616710294106 1103 34-35 2.650477989533158 1103 36-37 2.616116891886616 1103 38-39 2.748973784144617 1103 40-41 2.928162971070403 1103 42-43 2.986517350011674 1103 44-45 2.9043172543160374 1103 46-47 2.7226722207226466 1103 48-49 2.668572550254037 1103 50-51 2.8001295703659146 1103 52-53 2.7122913627486405 1103 54-55 3.0166219045620153 1103 56-57 2.933505482292187 1103 58-59 3.0281643497344497 1103 60-61 2.9206193642753817 1103 62-63 3.055165386030975 1103 64-65 3.154758863992356 1103 66-67 3.4220808110697156 1103 68-69 3.36559909375271 1103 70-71 3.1415048666530403 1103 72-73 3.19276750751515 1103 74-75 3.2035504805025745 1103 76-77 2.966470764163436 1103 78-79 3.104650745634718 1103 80-81 3.073053392157899 1103 82-83 3.0043432796143605 1103 84-85 3.1864408316377464 1103 86-87 3.0927041219439957 1103 88-89 3.07418157341111 1103 90-91 3.034679971233082 1103 92-93 2.8944869112666822 1103 94-95 2.7843383443524914 1103 96-97 2.764251274454608 1103 98-99 2.8962715988201886 1103 100-101 2.894381235276402 1103 102-103 2.6764735790923773 1103 104-105 2.741867978432275 1103 106-107 2.760200797805222 1103 108-109 2.80265414276489 1103 110-111 2.8149871502474184 1103 112-113 2.7572028520032283 1103 114-115 2.532891862121385 1103 116-117 2.6880155781023234 1103 118-119 2.735606012932486 1103 120-121 2.733815130918316 1103 122-123 2.834592583911455 1103 124-125 2.6824636341578554 1104 1 1.1486922577378635 1104 2 1.0264275775101126 1104 3 1.0312355918588345 1104 4 1.0850446725233027 1104 5 1.5478976771232702 1104 6 1.4655102945136278 1104 7 1.8206572156919414 1104 8 1.7600915434602058 1104 9 2.2769480186484863 1104 10-11 1.7453584456257971 1104 12-13 1.7785112204190199 1104 14-15 1.98528854645334 1104 16-17 1.7665533310577857 1104 18-19 1.6605733924216253 1104 20-21 2.1217333014033954 1104 22-23 2.1407146197750535 1104 24-25 2.226686381172236 1104 26-27 2.121080051246196 1104 28-29 2.1537996899541696 1104 30-31 2.6201040805359526 1104 32-33 2.51207292875025 1104 34-35 2.4192723131960463 1104 36-37 2.4082251579457967 1104 38-39 2.485356174549217 1104 40-41 2.688137157355481 1104 42-43 2.664305088126426 1104 44-45 2.3707182601199897 1104 46-47 2.4864769965022653 1104 48-49 2.1893024124788383 1104 50-51 2.6063386690838435 1104 52-53 2.356872758262419 1104 54-55 2.65692638436494 1104 56-57 2.620633406414097 1104 58-59 2.7397150818922817 1104 60-61 2.7286673177379903 1104 62-63 2.8356014356601094 1104 64-65 2.922142161697643 1104 66-67 2.8787875601329844 1104 68-69 3.000775526616504 1104 70-71 2.7662034325106433 1104 72-73 2.9231115030304338 1104 74-75 2.945822163686305 1104 76-77 2.8584600336586163 1104 78-79 2.8347978629244217 1104 80-81 2.667696843160897 1104 82-83 2.6811991607258605 1104 84-85 2.9064432316702806 1104 86-87 2.7741056839182434 1104 88-89 2.618257543600386 1104 90-91 2.725122534879837 1104 92-93 2.487423894231906 1104 94-95 2.4403772934518813 1104 96-97 2.4629723643359682 1104 98-99 2.5874965253142896 1104 100-101 2.5453305757361306 1104 102-103 2.463929314013594 1104 104-105 2.346103861213887 1104 106-107 2.537796707955902 1104 108-109 2.4191900497143415 1104 110-111 2.4506779470778213 1104 112-113 2.3601336859097515 1104 114-115 2.2108213603994677 1104 116-117 2.2799211066346388 1104 118-119 2.287754988304993 1104 120-121 2.2636451402564894 1104 122-123 2.3169852053546514 1104 124-125 2.0730585752784734 1105 1 0.8928105731193092 1105 2 0.962949403813063 1105 3 0.8992813422551862 1105 4 1.1231546932784298 1105 5 1.3152591394131683 1105 6 1.507924312951907 1105 7 1.7632579189158584 1105 8 1.552107947067661 1105 9 2.232030129508363 1105 10-11 1.9688358953066825 1105 12-13 2.0193691795845083 1105 14-15 1.9984984257468383 1105 16-17 1.919074819486525 1105 18-19 2.203893730592881 1105 20-21 2.435319372074794 1105 22-23 2.380462550759603 1105 24-25 2.4959419811588717 1105 26-27 2.262158092763883 1105 28-29 2.3318180019940087 1105 30-31 2.665767112270146 1105 32-33 2.508407290557834 1105 34-35 2.6423750566604056 1105 36-37 2.2912814715524696 1105 38-39 2.518051241214401 1105 40-41 2.6814181387501215 1105 42-43 2.667455282496917 1105 44-45 2.59917675542874 1105 46-47 2.5428658080389965 1105 48-49 2.6138822788885747 1105 50-51 2.7650175809061004 1105 52-53 2.6920880324046443 1105 54-55 2.8665447907125206 1105 56-57 2.624577215333229 1105 58-59 2.5503271911556027 1105 60-61 2.358987407144703 1105 62-63 2.184956021652816 1105 64-65 2.6231590145664185 1105 66-67 2.799761306192668 1105 68-69 2.8030967410329666 1105 70-71 2.7387049640833183 1105 72-73 2.930844311359216 1105 74-75 2.93935029449932 1105 76-77 2.7776950641601132 1105 78-79 2.867571550320225 1105 80-81 2.8406052903875434 1105 82-83 2.736762816599118 1105 84-85 2.942478574798251 1105 86-87 2.9002261267933633 1105 88-89 2.8118500004657285 1105 90-91 2.8399580903858848 1105 92-93 2.6501299510913086 1105 94-95 2.524224251284437 1105 96-97 2.5815369001673467 1105 98-99 2.6551437326539578 1105 100-101 2.6692116180223877 1105 102-103 2.3629438351789993 1105 104-105 2.0498002422468886 1105 106-107 2.321313495848866 1105 108-109 2.377965199994108 1105 110-111 2.484951610339756 1105 112-113 2.500729551220463 1105 114-115 2.322604055299056 1105 116-117 2.37217740093422 1105 118-119 2.391760617066627 1105 120-121 2.347867676837872 1105 122-123 2.4011200802855015 1105 124-125 2.288060394601164 1106 1 0.986727290644307 1106 2 0.8411565302390542 1106 3 0.9145906391583054 1106 4 0.8739139690557778 1106 5 1.0975191272071712 1106 6 1.2580521629746002 1106 7 1.5725013034204984 1106 8 1.312996950713817 1106 9 2.009519292771433 1106 10-11 1.6291861136062877 1106 12-13 1.6893346741622253 1106 14-15 1.8421984085332568 1106 16-17 1.7701311861672764 1106 18-19 1.8601382731026206 1106 20-21 1.707276870157834 1106 22-23 1.5693832587075889 1106 24-25 1.429044652394964 1106 26-27 1.5570899425987932 1106 28-29 1.4775517703616856 1106 30-31 1.9416992281332455 1106 32-33 1.7530868830652295 1106 34-35 1.708624997977715 1106 36-37 1.9111198202997919 1106 38-39 1.9919341575094123 1106 40-41 2.1780664964172125 1106 42-43 2.2197077589064804 1106 44-45 2.140666748073837 1106 46-47 2.0871291446585616 1106 48-49 1.9727443823891946 1106 50-51 2.287171079176918 1106 52-53 2.141398002202621 1106 54-55 2.5828307240802815 1106 56-57 2.2499865075417347 1106 58-59 2.2003419009501357 1106 60-61 2.4985583975520385 1106 62-63 2.4263764557482403 1106 64-65 2.6221334758593144 1106 66-67 2.642139363560865 1106 68-69 2.4344082600539814 1106 70-71 2.346076214259366 1106 72-73 2.281189287338435 1106 74-75 2.572146876819396 1106 76-77 2.2046254117181334 1106 78-79 2.1647096346042183 1106 80-81 2.25469203122913 1106 82-83 2.0722350166523213 1106 84-85 2.3052721056184 1106 86-87 2.3994552709649994 1106 88-89 2.112507246604409 1106 90-91 2.1809874630287958 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0.7418804308372664 2212 48-49 0.8944007235545754 2212 50-51 0.8524187108496015 2212 52-53 0.3814615784332247 2212 54-55 1.072990453723488 2212 56-57 0.5211441811025885 2212 58-59 0.8011380917870419 2212 60-61 0.6263785669984756 2212 62-63 0.4510178362091466 2212 64-65 0.6344019524195268 2212 66-67 0.7502099602305528 2212 68-69 0.7827295093945459 2212 70-71 0.679339726462846 2212 72-73 0.28387355695576133 2212 74-75 -0.26452973208758834 2212 76-77 0.09331154804180386 2212 78-79 0.646072713497361 2212 80-81 0.7158013688189158 2212 82-83 0.7034012346782106 2212 84-85 0.5262389603083903 2212 86-87 0.9224477951217125 2212 88-89 0.7919463779997926 2212 90-91 0.7772910913828923 2212 92-93 0.7835447068241095 2212 94-95 0.7492043111049753 2212 96-97 0.656540223530591 2212 98-99 0.9283806884532275 2212 100-101 0.8906888529841694 2212 102-103 0.8641269524937236 2212 104-105 0.7273841571687925 2212 106-107 0.435976174479638 2212 108-109 0.5815976360193638 2212 110-111 0.7771749403497274 2212 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32-33 -10.155586981308215 2216 34-35 -9.22130967565949 2216 36-37 -9.751362282578388 2216 38-39 -11.247041290180178 2216 40-41 -11.60612096677973 2216 42-43 -12.096524669034252 2216 44-45 -12.080971955741127 2216 46-47 -12.33690837129566 2216 48-49 -13.041301682336364 2216 50-51 -12.913821370404762 2216 52-53 -13.083784406458541 2216 54-55 -12.481552807877476 2216 56-57 -13.322197404627111 2216 58-59 -13.490789794964687 2216 60-61 -13.203881066087284 2216 62-63 -13.20442049299799 2216 64-65 -13.42624173449725 2216 66-67 -13.093854027581294 2216 68-69 -12.098682285184262 2216 70-71 -11.982376771981894 2216 72-73 -11.245838878770765 2216 74-75 -11.330802822927426 2216 76-77 -11.936473254852885 2216 78-79 -11.569910273870123 2216 80-81 -11.317457058147376 2216 82-83 -11.530858906247326 2216 84-85 -11.588456653225542 2216 86-87 -12.0412378520566 2216 88-89 -11.599859627205564 2216 90-91 -10.969774229508365 2216 92-93 -10.694740064330592 2216 94-95 -10.278235541621491 2216 96-97 -10.337375855946336 2216 98-99 -10.532322669637296 2216 100-101 -10.64575753817942 2216 102-103 -10.291720187733535 2216 104-105 -10.566712205428809 2216 106-107 -10.74760917434281 2216 108-109 -10.715718430761566 2216 110-111 -10.31395563795282 2216 112-113 -10.314631761844087 2216 114-115 -10.180040800396034 2216 116-117 -10.05157672993161 2216 118-119 -10.235901863655998 2216 120-121 -10.377481519020249 2216 122-123 -10.28466313187577 2216 124-125 -10.018415701455236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 159693.0 3 49777.0 4 24446.0 5 19833.0 6 20867.0 7 24200.0 8 26750.0 9 28443.0 10 30089.0 11 32005.0 12 33936.0 13 35501.0 14 35898.0 15 29465.0 16 21890.0 17 18903.0 18 16753.0 19 15947.0 20 15806.0 21 16813.0 22 20660.0 23 27803.0 24 38559.0 25 50485.0 26 60294.0 27 68475.0 28 77073.0 29 87324.0 30 97166.0 31 111851.0 32 129834.0 33 152659.0 34 185307.0 35 234163.0 36 322937.0 37 383052.0 38 122218.0 39 429.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.89686654264762 20.971136367256847 14.197855803967311 23.934141286128217 2 16.68714559777257 21.86972994691748 36.962772910936806 24.48035154437314 3 20.220712028137054 25.179782577324545 29.34035392020101 25.259151474337386 4 14.034980187861027 17.8460534445912 35.7026294308049 32.41633693674287 5 13.335672428575066 39.10127810804923 33.672113080164 13.890936383211711 6 31.723412736448836 37.58875507860318 16.715187583361246 13.972644601586742 7 27.656423625421745 33.13106920624184 20.698334823309573 18.514172345026847 8 25.857459968931533 36.2476408621075 19.2111637093146 18.683735459646364 9 25.8377945915968 16.181988212091802 19.646490083839588 38.33372711247181 10-11 24.96119526197553 27.12554201513708 26.87157148180249 21.041691241084905 12-13 25.747454193882003 24.375543142443117 27.48368848408922 22.393314179585662 14-15 22.6911211147748 25.738005993355845 25.620915379439204 25.949957512430156 16-17 22.239488910736053 28.80778674006748 26.282546808848917 22.670177540347545 18-19 22.088601002274103 27.140027811275928 28.75720593413329 22.01416525231668 20-21 23.02443919276086 26.280846542469043 28.821892293247675 21.87282197152242 22-23 22.524823783719928 26.18903655936265 28.767558625724348 22.518581031193072 24-25 22.271061001352567 26.410203535916605 28.803264412765067 22.51547104996576 26-27 22.211385127493458 26.63855545909264 28.774909376637538 22.375150036776365 28-29 22.08548324799699 26.59358371615609 28.976363554047683 22.344569481799237 30-31 22.195695024947945 26.44400798362529 28.76013294723882 22.600164044187945 32-33 22.16788894435988 26.365528143688856 28.82292261867182 22.643660293279446 34-35 22.48805875469378 26.47429216874661 28.580238147576797 22.457410928982817 36-37 22.545925283377237 26.248774220474964 28.65415818900304 22.551142307144758 38-39 22.369687607490203 26.503660303983406 28.62924511701566 22.49740697151073 40-41 22.502164188276865 26.416625917735203 28.532870460841675 22.548339433146257 42-43 22.494638419844577 26.450438520400176 28.29421727473096 22.76070578502429 44-45 22.542123554882114 26.554339544961618 28.150769043581846 22.752767856574415 46-47 22.647429474258313 26.796333322840404 27.72649604693664 22.829741155964648 48-49 22.775694225964777 26.835587336131404 27.51574070488011 22.87297773302371 50-51 22.77621275614109 26.99756083063376 27.289136037294455 22.937090375930698 52-53 22.995757085730496 26.91099146819123 27.169276575335775 22.923974870742494 54-55 22.67857683562327 26.848009530779454 27.158524818646217 23.314888814951058 56-57 22.730623182008532 27.080981262662274 27.155398656525527 23.032996898803663 58-59 22.78058885779527 26.88150973506917 27.163545200657584 23.174356206477974 60-61 22.831732986619055 26.786383777619953 27.43930260064004 22.942580635120947 62-63 22.748897135869147 26.83847384295427 27.39508311382159 23.017545907354993 64-65 22.632828002615586 26.767660862746872 27.322446619695583 23.277064514941955 66-67 22.616756106008353 27.010315624355474 27.066087707747915 23.30684056188826 68-69 22.615862651957542 26.815638407368237 27.295801249969053 23.27269769070517 70-71 22.498348658898806 26.73830289000167 27.46724508554699 23.29610336555254 72-73 22.60311037950509 26.857469784384254 27.12827687552005 23.411142960590606 74-75 22.602025882841893 27.0562091850044 26.95605576262419 23.38570916952952 76-77 22.6755152778608 26.825415127927698 27.2015211895895 23.297548404622002 78-79 22.69794317922976 26.931222844886737 27.065769312935846 23.305064662947657 80-81 22.475520377122983 27.03798199024122 27.086774115450407 23.399723517185386 82-83 22.67176198909277 26.993324724410115 27.000557776167287 23.334355510329825 84-85 22.67584643813443 26.900065734011225 26.85555335675848 23.56853447109587 86-87 22.547210219216137 26.83168505935519 27.10365822386792 23.517446497560748 88-89 22.614103224110984 26.822571765645055 26.976235602084053 23.587089408159905 90-91 22.64384982478851 27.045438267462124 26.95811105835166 23.352600849397703 92-93 22.503492370371116 26.844536595744007 27.136783312456043 23.515187721428838 94-95 22.3721731588747 26.703037368632533 27.2015369356929 23.72325253679987 96-97 22.509039300730837 26.609083955628066 27.16780956675907 23.71406717688203 98-99 22.328232125020868 26.75276517841732 26.896506353756088 24.022496342805727 100-101 22.275333779581242 26.71126198259633 27.069946264560162 23.943457973262262 102-103 22.300222227802774 26.375819294806067 27.306608226496216 24.017350250894943 104-105 22.256941251370566 26.391380468998694 27.30442471616029 24.04725356347045 106-107 22.087484448515855 26.548202748025723 27.220840359317762 24.14347244414066 108-109 22.19190083556632 26.407896002693732 27.04028289847855 24.359920263261397 110-111 22.05300499646394 26.543243826839003 27.128767221159272 24.274983955537785 112-113 22.050987124827838 26.459394857712404 27.21331148918651 24.276306528273246 114-115 21.97466557304339 26.564187233115423 27.050552488435077 24.410594705406112 116-117 21.746989358059835 26.502102356166862 27.27175783007416 24.479150455699138 118-119 21.758006335779324 26.489697770982072 27.073315963260047 24.67897992997855 120-121 21.73886669604752 26.528063785220766 27.19277612991902 24.540293388812685 122-123 21.780325504873165 26.51961548059002 26.932275115619504 24.767783898917312 124-125 21.67672166496057 26.420987733798164 26.976341277583742 24.92594932365752 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 267.0 1 209.5 2 251.0 3 394.5 4 342.0 5 292.5 6 578.0 7 1241.0 8 1882.0 9 2246.5 10 2045.5 11 1591.5 12 1514.5 13 1668.5 14 1898.5 15 2143.0 16 2400.0 17 2625.5 18 2981.0 19 3359.0 20 3888.0 21 4717.5 22 5645.5 23 7083.5 24 8765.0 25 10878.0 26 14280.0 27 18206.0 28 22142.5 29 26554.5 30 32039.5 31 38257.5 32 44453.5 33 50092.0 34 55580.0 35 60339.0 36 66222.0 37 72718.5 38 77124.5 39 80174.0 40 82228.5 41 84758.0 42 86647.5 43 89702.0 44 94403.0 45 98640.0 46 101226.5 47 102254.5 48 104440.0 49 104647.0 50 101655.5 51 98882.0 52 97945.5 53 97956.0 54 96929.5 55 92083.5 56 84291.0 57 77176.0 58 72004.0 59 66047.5 60 57470.5 61 47472.0 62 37955.5 63 29960.5 64 22807.0 65 16892.0 66 12648.0 67 9862.5 68 7474.0 69 5454.5 70 4116.5 71 3212.5 72 2441.5 73 1996.0 74 1569.5 75 1094.5 76 753.0 77 465.0 78 270.0 79 163.0 80 107.0 81 66.0 82 51.0 83 37.0 84 26.0 85 23.0 86 14.5 87 7.0 88 4.0 89 3.5 90 2.0 91 1.5 92 2.5 93 1.0 94 1.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08732700834434501 2 2.8295506956450383E-4 3 0.0 4 0.1694900866691378 5 0.0 6 3.536938369556298E-5 7 0.022530297414073617 8 0.0 9 0.0 10-11 2.6527037771672233E-4 12-13 2.475856858689409E-4 14-15 2.299009940211594E-4 16-17 0.0030948210733617613 18-19 0.0011848743538013598 20-21 0.0022105864809726867 22-23 9.01919284236856E-4 24-25 0.0031125057652095422 26-27 0.00284723538749282 28-29 0.0021221630217337787 30-31 6.012795228245707E-4 32-33 1.768469184778149E-5 34-35 8.3118051684573E-4 36-37 7.604417494546041E-4 38-39 4.0674791249897433E-4 40-41 0.001114135586410234 42-43 4.421172961945372E-4 44-45 9.196039760846376E-4 46-47 1.0610815108668894E-4 48-49 1.768469184778149E-5 50-51 0.03296426560426469 52-53 7.073876739112596E-5 54-55 0.12770115983283015 56-57 2.8295506956450383E-4 58-59 0.0 60-61 0.0 62-63 2.299009940211594E-4 64-65 0.041311440156417564 66-67 0.02196438727494461 68-69 0.0012025590456491413 70-71 0.0019983701787993084 72-73 0.06582242305744271 74-75 0.005004767792922162 76-77 0.0015208834989092082 78-79 9.903427434757636E-4 80-81 1.2379284293447044E-4 82-83 1.7684691847781492E-4 84-85 1.768469184778149E-5 86-87 1.0610815108668894E-4 88-89 0.0010080274353235449 90-91 2.299009940211594E-4 92-93 0.0024581721668416275 94-95 0.004244326043467557 96-97 0.0023520640157549382 98-99 0.0 100-101 9.372886679324189E-4 102-103 8.842345923890744E-4 104-105 1.4147753478225192E-4 106-107 5.305407554334447E-5 108-109 0.0 110-111 1.5916222663003342E-4 112-113 7.073876739112596E-5 114-115 1.7684691847781492E-4 116-117 0.0 118-119 9.72658051627982E-4 120-121 0.002334379323907157 122-123 0.0012202437374969229 124-125 0.006578705367374714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2827304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.25100248634058 #Duplication Level Percentage of deduplicated Percentage of total 1 77.18598344134526 42.64602963028409 2 11.246540918382378 12.427653204885516 3 4.530942774066412 7.510173914262307 4 2.2430373964327446 4.95720259069042 5 1.257282333384269 3.473305466392317 6 0.8263690928899399 2.739463248353825 7 0.5334873122549857 2.0632996171081954 8 0.3858058081407953 1.7052926131865376 9 0.2728327102615881 1.356685267771624 >10 1.3960864785174623 13.68984785240637 >50 0.08756205817149985 3.3003556492668196 >100 0.03270476578612709 3.107161513154019 >500 0.0010399317078526899 0.3447795270436799 >1k 1.949871951921099E-4 0.23762171962703177 >5k 1.299914634614066E-4 0.44112818556724476 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6191 0.21897185445923043 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6093 0.21550565485706522 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3683 0.13026544015075847 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.073876739112596E-5 0.0 0.0 0.0 0.0 7 1.4147753478225192E-4 0.0 0.0 0.0 0.0 8 1.7684691847781492E-4 0.0 0.0 0.0 0.0 9 1.7684691847781492E-4 0.0 0.0 0.0 0.0 10-11 1.7684691847781492E-4 0.0 0.0 5.305407554334447E-5 0.0 12-13 2.299009940211594E-4 0.0 0.0 7.073876739112596E-5 0.0 14-15 2.8295506956450383E-4 0.0 0.0 7.073876739112596E-5 3.536938369556298E-5 16-17 3.3600914510784833E-4 0.0 0.0 8.842345923890744E-5 3.536938369556298E-5 18-19 3.5369383695562983E-4 0.0 0.0 1.0610815108668894E-4 3.536938369556298E-5 20-21 3.5369383695562983E-4 0.0 0.0 2.475856858689409E-4 3.536938369556298E-5 22-23 3.5369383695562983E-4 0.0 0.0 5.659101391290078E-4 3.536938369556298E-5 24-25 4.951713717378818E-4 0.0 0.0 9.903427434757636E-4 3.536938369556298E-5 26-27 4.951713717378818E-4 0.0 0.0 0.0014147753478225191 3.536938369556298E-5 28-29 5.659101391290078E-4 0.0 0.0 0.002192901789124905 3.536938369556298E-5 30-31 6.366489065201337E-4 0.0 0.0 0.003979055665750835 3.536938369556298E-5 32-33 6.366489065201337E-4 0.0 0.0 0.008258751092913956 3.536938369556298E-5 34-35 7.958111331501671E-4 0.0 0.0 0.013670266798335093 3.536938369556298E-5 36-37 9.903427434757636E-4 0.0 0.0 0.02528910934232753 3.536938369556298E-5 38-39 0.0010610815108668896 0.0 0.0 0.046422316100426415 3.536938369556298E-5 40-41 0.001078766202714671 0.0 0.0 0.06987221749058467 3.536938369556298E-5 42-43 0.0011141355864102338 0.0 0.0 0.09406487593834975 3.536938369556298E-5 44-45 0.0012379284293447044 0.0 0.0 0.12129930138393324 3.536938369556298E-5 46-47 0.0013970906559747378 0.0 0.0 0.1556429729523249 3.536938369556298E-5 48-49 0.0014501447315180822 0.0 0.0 0.1967245121147213 3.536938369556298E-5 50-51 0.0014501447315180822 0.0 0.0 0.2404941244379805 3.536938369556298E-5 52-53 0.0014501447315180822 0.0 0.0 0.283344132785155 3.536938369556298E-5 54-55 0.0014855141152136453 0.0 0.0 0.3318355578317719 3.536938369556298E-5 56-57 0.0015208834989092082 0.0 0.0 0.3913445458995566 3.536938369556298E-5 58-59 0.0015208834989092082 0.0 0.0 0.46019105126296994 3.536938369556298E-5 60-61 0.0015208834989092082 0.0 0.0 0.5396837411187478 3.536938369556298E-5 62-63 0.0015208834989092082 0.0 0.0 0.6192471697419166 3.536938369556298E-5 64-65 0.0015208834989092082 0.0 0.0 0.6998009411085614 3.536938369556298E-5 66-67 0.001626991649995897 0.0 0.0 0.7973850707246197 3.536938369556298E-5 68-69 0.0016800457255392418 0.0 0.0 0.912441675886286 3.536938369556298E-5 70-71 0.001697730417387023 0.0 0.0 1.042247314049002 3.536938369556298E-5 72-73 0.0017330998010825862 0.0 0.0 1.175890530342687 3.536938369556298E-5 74-75 0.0017861538766259307 0.0 0.0 1.3123632973320167 3.536938369556298E-5 76-77 0.001803838568473712 0.0 0.0 1.4680062702843415 3.536938369556298E-5 78-79 0.001803838568473712 0.0 0.0 1.6434737827980295 3.536938369556298E-5 80-81 0.0022990099402115938 0.0 0.0 1.844637152566544 3.536938369556298E-5 82-83 0.0022990099402115938 0.0 0.0 2.0545544447997104 3.536938369556298E-5 84-85 0.0023874333994505013 0.0 0.0 2.2691581803725387 3.536938369556298E-5 86-87 0.00249354155053719 0.0 0.0 2.4991829672366324 3.536938369556298E-5 88-89 0.0025112262423849716 0.0 0.0 2.759802270997388 3.536938369556298E-5 90-91 0.0027764966201016938 0.0 0.0 3.056551400203162 3.536938369556298E-5 92-93 0.0029002894630361644 0.0 0.0 3.373125068970298 3.536938369556298E-5 94-95 0.0029002894630361644 0.0 0.0 3.6962774431048095 3.536938369556298E-5 96-97 0.0029002894630361644 0.0 0.0 4.023992467736048 3.536938369556298E-5 98-99 0.0031478751489051053 0.0 0.0 4.387112245446546 3.536938369556298E-5 100-101 0.003253983299991794 0.0 0.0 4.805408261722121 3.536938369556298E-5 102-103 0.0033424067592307018 0.0 0.0 5.263494834655205 3.536938369556298E-5 104-105 0.003926001590207491 0.0 0.0 5.716576639795367 3.536938369556298E-5 106-107 0.004208956659771995 0.0 0.0 6.16407008231163 3.536938369556298E-5 108-109 0.004279695427163121 0.0 0.0 6.643997249676724 3.536938369556298E-5 110-111 0.004279695427163121 0.0 0.0 7.17276953592539 3.536938369556298E-5 112-113 0.0043150648108586835 0.0 0.0 7.739705387181569 3.536938369556298E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5625 0.0 62.442757 1 GTATCAA 9550 0.0 47.269585 1 ATCAACG 12320 0.0 35.300903 3 TATCAAC 12645 0.0 35.28878 2 TCAACGC 12355 0.0 34.76692 4 CAACGCA 12420 0.0 34.102505 5 AACGCAG 12975 0.0 32.643784 6 GTACATG 11600 0.0 29.713865 1 TACATGG 11975 0.0 28.059326 2 CGCAGAG 15505 0.0 27.157482 8 ACGCAGA 15475 0.0 27.062622 7 ACATGGG 11935 0.0 26.006922 3 GCAGAGT 16570 0.0 24.299324 9 GAGTACT 9715 0.0 23.753756 12-13 GTACTTT 10280 0.0 22.535006 14-15 AGAGTAC 15865 0.0 22.212204 10-11 CAGAGTA 16555 0.0 22.112722 10-11 AGTACTT 10210 0.0 22.077854 12-13 GTGGTAT 3220 0.0 21.668022 1 TGGTATC 3130 0.0 20.937363 2 >>END_MODULE