FastQCFastQC Report
Thu 26 May 2016
SRR1364505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572411
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36370.1413848720130648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32540.1264961159006084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85500.050.20121
GGTATCA48000.046.7568321
TCAACGC103950.039.783474
ATCAACG104200.039.632443
CAACGCA104300.039.5920645
GTACATG141100.038.8577421
AACGCAG104550.038.391316
TATCAAC115150.037.517312
TACATGG149650.035.4688572
ACATGGG148050.033.0788543
ACGCAGA120150.032.614277
CGCAGAG118750.031.9970198
CATGGGA91450.028.6705934
GCAGAGT123700.028.6469359
CATGGGG55850.027.313954
GAGTACT49650.025.03876712-13
ATGGGAT41900.024.74545
GAGTACA106100.024.4633561
CATGGGT21250.023.5552674
ATGGGAG35250.023.328095
Produced by FastQC (version 0.11.5)