##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364505_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2572411 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.44312786720318 30.0 25.0 31.0 2.0 34.0 2 25.74298275042363 30.0 25.0 31.0 2.0 34.0 3 25.67875701044662 30.0 25.0 31.0 2.0 34.0 4 28.345636447674963 35.0 26.0 37.0 2.0 37.0 5 29.046849434246706 35.0 28.0 37.0 2.0 37.0 6 29.103458584184253 35.0 28.0 37.0 2.0 37.0 7 28.90943904376089 35.0 28.0 37.0 2.0 37.0 8 28.517336848582904 35.0 27.0 37.0 2.0 37.0 9 29.020572529039878 35.0 26.0 39.0 2.0 39.0 10-11 29.106805250016425 35.0 26.5 39.0 2.0 39.0 12-13 28.96084840252977 35.0 26.0 39.0 2.0 39.0 14-15 29.564540814045657 36.0 25.5 40.0 2.0 41.0 16-17 29.81990397335418 36.0 27.0 40.0 2.0 41.0 18-19 29.775480084636552 36.0 27.0 40.0 2.0 41.0 20-21 29.557131616992777 36.0 25.5 40.0 2.0 41.0 22-23 29.59221621272806 36.0 26.0 40.0 2.0 41.0 24-25 29.616380314032245 36.0 26.0 40.0 2.0 41.0 26-27 29.717761080947017 36.0 26.5 40.0 2.0 41.0 28-29 29.64443220776151 36.0 26.5 40.0 2.0 41.0 30-31 29.28644275739763 35.0 25.0 40.0 2.0 41.0 32-33 29.269954529039097 35.0 25.0 40.0 2.0 41.0 34-35 28.966143435088718 35.0 24.5 40.0 2.0 41.0 36-37 28.721175387603303 35.0 24.0 40.0 2.0 41.0 38-39 28.559731706947296 35.0 24.0 39.0 2.0 41.0 40-41 28.59964406931863 35.0 24.0 40.0 2.0 41.0 42-43 28.430091264576305 35.0 24.0 39.0 2.0 41.0 44-45 28.06925467975374 34.0 22.5 39.0 2.0 41.0 46-47 27.786233809449577 34.0 21.0 39.0 2.0 40.5 48-49 27.522333134168683 33.5 19.5 39.0 2.0 40.0 50-51 27.48984318602276 33.5 19.5 39.0 2.0 40.0 52-53 27.284264062002535 33.0 18.5 39.0 2.0 40.0 54-55 27.14832233262881 33.0 18.0 39.0 2.0 40.0 56-57 26.733077645834975 33.0 14.5 38.0 2.0 40.0 58-59 26.50264401761616 33.0 13.5 38.0 2.0 40.0 60-61 26.096202745206732 32.5 12.0 38.0 2.0 40.0 62-63 26.035476834767074 32.0 12.5 38.0 2.0 40.0 64-65 25.852453787516847 32.0 10.0 37.0 2.0 40.0 66-67 25.618000778258217 32.0 8.0 37.0 2.0 40.0 68-69 25.443877164263412 32.0 8.0 36.5 2.0 39.5 70-71 25.320322257990654 32.0 7.5 36.0 2.0 39.0 72-73 25.28423043596066 32.0 7.0 36.0 2.0 39.0 74-75 25.013428452918294 32.0 6.5 35.5 2.0 39.0 76-77 24.755661517541327 32.0 2.0 35.0 2.0 38.5 78-79 24.45734118692542 32.0 2.0 35.0 2.0 37.0 80-81 24.449975723163988 32.0 2.0 35.0 2.0 37.0 82-83 24.25354638119647 32.0 2.0 35.0 2.0 36.0 84-85 24.061514081536735 32.0 2.0 35.0 2.0 36.0 86-87 23.859546549909794 31.0 2.0 35.0 2.0 36.0 88-89 23.64987943217472 31.0 2.0 35.0 2.0 35.0 90-91 23.488693680753194 31.0 2.0 35.0 2.0 35.0 92-93 23.382336065271062 31.0 2.0 35.0 2.0 35.0 94-95 23.29882685931603 31.0 2.0 35.0 2.0 35.0 96-97 23.176672778961063 31.0 2.0 35.0 2.0 35.0 98-99 23.06793121316928 31.0 2.0 35.0 2.0 35.0 100-101 23.006916468635843 31.0 2.0 35.0 2.0 35.0 102-103 21.920830497148394 29.5 2.0 33.5 2.0 34.5 104-105 22.652035191888075 30.5 2.0 34.0 2.0 35.0 106-107 22.746078678718135 31.0 2.0 34.5 2.0 35.0 108-109 22.671562592447316 31.0 2.0 35.0 2.0 35.0 110-111 22.541486372123273 31.0 2.0 34.5 2.0 35.0 112-113 22.516472484373608 31.0 2.0 35.0 2.0 35.0 114-115 22.459741853070913 31.0 2.0 35.0 2.0 35.0 116-117 22.280149245202264 31.0 2.0 35.0 2.0 35.0 118-119 22.112683004387712 31.0 2.0 34.5 2.0 35.0 120-121 21.952531302346323 31.0 2.0 34.0 2.0 35.0 122-123 21.67085003135191 30.5 2.0 34.0 2.0 35.0 124-125 20.70051267079794 28.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.815251430924576 1101 2 1.3295569287026616 1101 3 1.6850440507174298 1101 4 2.164998164713058 1101 5 1.551712180313789 1101 6 1.5533659486172056 1101 7 1.2122703980545317 1101 8 1.613934884035416 1101 9 2.2602624851190036 1101 10-11 2.2006693051411013 1101 12-13 1.9838355812622908 1101 14-15 2.4340340745540345 1101 16-17 1.973738709903742 1101 18-19 1.984437687780872 1101 20-21 2.35945163570808 1101 22-23 2.1656964076794267 1101 24-25 2.0496948835288613 1101 26-27 1.8030810513088156 1101 28-29 1.7504854666790521 1101 30-31 1.908568993572242 1101 32-33 2.1218276959480704 1101 34-35 2.4140851184372885 1101 36-37 2.382808073622247 1101 38-39 2.3825367977807126 1101 40-41 2.2420377746079403 1101 42-43 2.129082833946619 1101 44-45 2.347192008424031 1101 46-47 2.3397850583216204 1101 48-49 2.3594025182824474 1101 50-51 2.2242122984874584 1101 52-53 2.105301361560887 1101 54-55 2.113258918809475 1101 56-57 2.3325925526447513 1101 58-59 2.4025977501488676 1101 60-61 2.5584216826598727 1101 62-63 2.165474701680509 1101 64-65 2.0907362504172298 1101 66-67 2.1064711873945434 1101 68-69 2.014623400655818 1101 70-71 2.0191414285741622 1101 72-73 1.9482664916893526 1101 74-75 1.9585112794807316 1101 76-77 2.186114505257315 1101 78-79 2.0903930128276826 1101 80-81 1.944175686282513 1101 82-83 1.8284034063960917 1101 84-85 1.6977973339388939 1101 86-87 1.750546273351837 1101 88-89 1.9189011597345917 1101 90-91 1.8222120231898913 1101 92-93 1.8636126734552576 1101 94-95 1.862460400236781 1101 96-97 1.7625804107174972 1101 98-99 1.6586924357356096 1101 100-101 1.6735362189321101 1101 102-103 1.726988834923631 1101 104-105 1.722563851028088 1101 106-107 1.820166034640085 1101 108-109 1.9193555841975787 1101 110-111 1.9114225114430106 1101 112-113 1.830474236961578 1101 114-115 1.7969545691977302 1101 116-117 1.892363939948801 1101 118-119 1.811377769473129 1101 120-121 1.8055979948690961 1101 122-123 1.7987238560227041 1101 124-125 1.8079589024948497 1102 1 0.8204663389585285 1102 2 0.5468409409728174 1102 3 0.6071241322471863 1102 4 1.1004032194469353 1102 5 1.1289428505205628 1102 6 1.0216920639407512 1102 7 0.9155625688490119 1102 8 0.9853230816414964 1102 9 1.3612652382279258 1102 10-11 0.9788131160098281 1102 12-13 1.3463659260343874 1102 14-15 1.513809497808694 1102 16-17 1.0257332573238926 1102 18-19 1.2572696723035328 1102 20-21 1.3588575486499117 1102 22-23 1.202972503721032 1102 24-25 0.9656793765989242 1102 26-27 1.115304041109269 1102 28-29 0.8560997003539086 1102 30-31 0.830092812079755 1102 32-33 0.7730594740569217 1102 34-35 1.2395152628067336 1102 36-37 1.417354365525778 1102 38-39 1.4827804807505558 1102 40-41 1.0237462927842849 1102 42-43 1.0458683827604354 1102 44-45 1.403639156963365 1102 46-47 1.3081673085835668 1102 48-49 1.5484460325700375 1102 50-51 1.3742410133117957 1102 52-53 1.3982112506504585 1102 54-55 1.2431151227374393 1102 56-57 1.4131071629878207 1102 58-59 1.4096909128763784 1102 60-61 1.7622845432482421 1102 62-63 1.6170058353010823 1102 64-65 1.7004335379067257 1102 66-67 1.7770656351220033 1102 68-69 1.579809381163031 1102 70-71 1.7153229580524005 1102 72-73 1.6401209144658964 1102 74-75 1.6961974287626802 1102 76-77 1.4966268128656814 1102 78-79 1.3638643992681807 1102 80-81 0.9498000926809738 1102 82-83 0.9877305454655421 1102 84-85 1.06109386975659 1102 86-87 1.1408171729812722 1102 88-89 0.8891757215445217 1102 90-91 0.8789877507014658 1102 92-93 0.9127463735615144 1102 94-95 1.073717813609104 1102 96-97 1.0980353117970054 1102 98-99 1.2580657034826217 1102 100-101 1.1498176815478125 1102 102-103 0.934868996540466 1102 104-105 0.9236553842702335 1102 106-107 1.2148483820977845 1102 108-109 1.2778051871376803 1102 110-111 1.013335260917497 1102 112-113 0.9470649318788205 1102 114-115 1.0431936670436102 1102 116-117 1.2706807795980097 1102 118-119 1.3826513852197664 1102 120-121 1.2259888494056206 1102 122-123 1.394888091870829 1102 124-125 1.2956360350445166 1103 1 1.5768761232809574 1103 2 1.3028937277568495 1103 3 1.513773357156861 1103 4 2.0166898436077574 1103 5 1.4620946471995104 1103 6 1.561485107245037 1103 7 1.6570207414659421 1103 8 1.8789319849158481 1103 9 2.5348815428952847 1103 10-11 2.133104972697744 1103 12-13 2.190520942871636 1103 14-15 2.3956847076222303 1103 16-17 2.2526699398182473 1103 18-19 1.9705293660724301 1103 20-21 2.329064118705695 1103 22-23 2.1609623872564896 1103 24-25 2.1769858636191763 1103 26-27 1.9636838663602951 1103 28-29 1.8500774151646127 1103 30-31 2.0202287374963 1103 32-33 2.020392640459999 1103 34-35 1.8296193364573448 1103 36-37 2.2526473189209355 1103 38-39 2.2958105840447907 1103 40-41 2.199242066441588 1103 42-43 2.1605437617135976 1103 44-45 2.086880647969693 1103 46-47 2.232320367672582 1103 48-49 2.0756726336429345 1103 50-51 2.0999558829312868 1103 52-53 2.2016143878345993 1103 54-55 2.034653139569169 1103 56-57 2.1634888625219517 1103 58-59 2.4213033057295554 1103 60-61 2.376455418459898 1103 62-63 2.0523731401691236 1103 64-65 2.0126795292791293 1103 66-67 2.1006371079685273 1103 68-69 1.9743842705563885 1103 70-71 1.9889465966042046 1103 72-73 1.978137746190992 1103 74-75 1.9685016431993247 1103 76-77 2.079932467689506 1103 78-79 2.201514659513677 1103 80-81 1.891456249492311 1103 82-83 1.9241252999277059 1103 84-85 1.8290682385975856 1103 86-87 1.9059320940300104 1103 88-89 1.9237941416846596 1103 90-91 1.913118298754366 1103 92-93 1.940391497019327 1103 94-95 1.7154643091477055 1103 96-97 1.8010673973880458 1103 98-99 1.7504743045641078 1103 100-101 1.5734212516459394 1103 102-103 1.6623994781894886 1103 104-105 1.826024259632863 1103 106-107 1.5670088708106569 1103 108-109 1.9316026970537692 1103 110-111 2.101916095973831 1103 112-113 1.9589490773708178 1103 114-115 1.8671927145592022 1103 116-117 1.9540007646479296 1103 118-119 2.052487087796692 1103 120-121 1.9733793060273825 1103 122-123 1.9777346884881588 1103 124-125 1.8558374069411947 1104 1 1.1941957434238404 1104 2 0.9456188770377985 1104 3 0.9695912318959508 1104 4 1.1916162972533044 1104 5 0.8848595239245043 1104 6 0.7936886726916477 1104 7 0.8011745099612142 1104 8 1.0728705252102522 1104 9 1.63021167427231 1104 10-11 1.509547038939754 1104 12-13 0.9232513171168399 1104 14-15 1.324918347674057 1104 16-17 0.9815863990151001 1104 18-19 0.39994668990005167 1104 20-21 -4.079090088952313 1104 22-23 -0.9399425641984251 1104 24-25 0.5771889006252771 1104 26-27 0.7364001939248794 1104 28-29 0.39403789028554215 1104 30-31 1.1165351259970429 1104 32-33 1.0000774887513018 1104 34-35 1.2636827758917555 1104 36-37 1.3106018520581983 1104 38-39 1.2711793298074454 1104 40-41 1.2747530272697425 1104 42-43 1.2811717877318927 1104 44-45 1.376115986164038 1104 46-47 1.3700627157456466 1104 48-49 1.4872753701582333 1104 50-51 1.3066331727983886 1104 52-53 1.3684580269039088 1104 54-55 1.363410763582234 1104 56-57 1.618870954496007 1104 58-59 1.6163977367894553 1104 60-61 1.7170296435468835 1104 62-63 1.2459676230357744 1104 64-65 1.356140101311901 1104 66-67 1.3146758514922467 1104 68-69 1.3056417596621124 1104 70-71 1.6419686725735332 1104 72-73 1.4278548953208912 1104 74-75 1.4321559445057517 1104 76-77 1.4322382118984258 1104 78-79 1.3338952420406684 1104 80-81 1.200598142062244 1104 82-83 1.1362159289898592 1104 84-85 1.1029253053471706 1104 86-87 1.069931548340012 1104 88-89 1.256547587124924 1104 90-91 1.2696693118531392 1104 92-93 0.8740666189283601 1104 94-95 1.0541636135619576 1104 96-97 1.1649999982628962 1104 98-99 1.0420690934926853 1104 100-101 0.6414641441385989 1104 102-103 1.086645421907427 1104 104-105 1.198960133598753 1104 106-107 1.3803394178071926 1104 108-109 1.4031123061278379 1104 110-111 1.3820853402934183 1104 112-113 1.358382559776274 1104 114-115 1.3573140006300193 1104 116-117 1.4157800870063149 1104 118-119 1.2816288684780304 1104 120-121 1.3360035970645328 1104 122-123 1.1943943349225314 1104 124-125 1.2479692844329726 1105 1 1.1290121821715324 1105 2 0.8936026138823792 1105 3 0.9121764378342512 1105 4 1.459634166820976 1105 5 1.0181126231127955 1105 6 1.1432136290913029 1105 7 1.029230752312511 1105 8 1.1404730197713349 1105 9 1.7084116671740972 1105 10-11 1.3954199614014762 1105 12-13 1.3940521744674577 1105 14-15 1.5604725758197375 1105 16-17 1.4102399817070648 1105 18-19 1.075215092452531 1105 20-21 1.303586074302281 1105 22-23 1.2215248599093833 1105 24-25 1.189982646559578 1105 26-27 1.1600592954164313 1105 28-29 0.7190517359225765 1105 30-31 1.0908719415017032 1105 32-33 1.0636567306600782 1105 34-35 0.8196495704166082 1105 36-37 1.165592695720587 1105 38-39 1.1517738131774102 1105 40-41 1.3840669993893364 1105 42-43 1.610023766582053 1105 44-45 1.7883180809378842 1105 46-47 2.0197645024783846 1105 48-49 1.6671914497388372 1105 50-51 1.7112846937078672 1105 52-53 1.687980664004602 1105 54-55 1.7249130950849256 1105 56-57 2.0624108520388837 1105 58-59 1.6810727501811336 1105 60-61 2.054244216196299 1105 62-63 2.0426416575347304 1105 64-65 1.6730775513399685 1105 66-67 1.843283378354318 1105 68-69 1.4427735237878778 1105 70-71 1.518443629391772 1105 72-73 1.443732542455173 1105 74-75 1.8090432637180456 1105 76-77 1.658723562524667 1105 78-79 1.5642606725300254 1105 80-81 1.4415099567032108 1105 82-83 1.5540137599324204 1105 84-85 1.4775911618116915 1105 86-87 1.5084940027455076 1105 88-89 1.557074110436826 1105 90-91 1.6329391213133562 1105 92-93 1.6189596216611726 1105 94-95 1.4418828371309402 1105 96-97 1.6280167600616977 1105 98-99 1.3012750419344457 1105 100-101 1.4053208352807296 1105 102-103 1.4176191112470597 1105 104-105 1.5512558091968636 1105 106-107 1.619555326146422 1105 108-109 1.6956176159311127 1105 110-111 1.5528753852538593 1105 112-113 1.6006664396108867 1105 114-115 1.4411207838071647 1105 116-117 1.628324057294762 1105 118-119 1.6065711719368991 1105 120-121 1.6275171324201665 1105 122-123 1.7542128223646536 1105 124-125 1.483836653311517 1106 1 -0.4647123330998717 1106 2 -0.2734737846404798 1106 3 -0.14294567148115433 1106 4 -0.5669713957830211 1106 5 -1.1222027518445508 1106 6 -0.48605940646841717 1106 7 -0.3209602143516541 1106 8 -0.1403876279211893 1106 9 0.04795948657061899 1106 10-11 -2.3707245935783483 1106 12-13 -0.46489895150466864 1106 14-15 -0.17769625214953777 1106 16-17 -0.36205944400960277 1106 18-19 -3.018246917763097 1106 20-21 -5.37170175903303 1106 22-23 -6.212896304914807 1106 24-25 -6.103420934048909 1106 26-27 -5.211253941293958 1106 28-29 -1.7462033979121827 1106 30-31 -3.4553220316193602 1106 32-33 -4.174920182333164 1106 34-35 -3.4820856637563473 1106 36-37 -5.8156561432626965 1106 38-39 -6.165340198993338 1106 40-41 -2.5557900292247027 1106 42-43 -1.1760998275172803 1106 44-45 -0.40080874227006547 1106 46-47 -0.2247367247409855 1106 48-49 -0.05566941401807313 1106 50-51 -0.0986629633768743 1106 52-53 -0.2985225578579538 1106 54-55 -0.3033758594137588 1106 56-57 -0.03600351202697638 1106 58-59 -0.027584844416200127 1106 60-61 0.16932353422331659 1106 62-63 0.010649306741022713 1106 64-65 -0.18443193770728072 1106 66-67 -0.5549486484482067 1106 68-69 -0.41786033534604883 1106 70-71 -0.14477819363832012 1106 72-73 -0.06588956883642894 1106 74-75 -0.07203437058770845 1106 76-77 -0.0730809176494489 1106 78-79 -0.1638246017597602 1106 80-81 -0.4709105177689139 1106 82-83 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34-35 0.5320341406961298 2213 36-37 0.48385098612932964 2213 38-39 0.13107471690339167 2213 40-41 0.4159980133659431 2213 42-43 0.25381506485010163 2213 44-45 0.5089033475896514 2213 46-47 0.4182584864652874 2213 48-49 0.7158908994000441 2213 50-51 0.46594094986037504 2213 52-53 0.4129803018302347 2213 54-55 -1.579896592378784 2213 56-57 -0.8097380935349783 2213 58-59 0.21211008406481469 2213 60-61 0.7112684158562601 2213 62-63 0.09440415237225963 2213 64-65 0.29225363909208824 2213 66-67 0.5145496396865141 2213 68-69 0.17016279995925032 2213 70-71 0.24790204928082105 2213 72-73 0.4905699408890172 2213 74-75 0.3833831742170446 2213 76-77 0.3930225912561376 2213 78-79 0.21678559784492535 2213 80-81 -0.07249188243009641 2213 82-83 -0.03486673891348957 2213 84-85 0.17701434272859373 2213 86-87 0.3531409112369879 2213 88-89 0.3693309934716069 2213 90-91 0.4442681254953449 2213 92-93 0.4532802264852407 2213 94-95 0.29678777051579175 2213 96-97 0.11176973671937773 2213 98-99 0.22250455147435844 2213 100-101 0.43411969408711215 2213 102-103 0.45515616880950915 2213 104-105 0.49098607597806065 2213 106-107 0.5074602222451254 2213 108-109 0.5186645639411651 2213 110-111 0.5733619267356502 2213 112-113 0.5585242459171837 2213 114-115 0.36301752540157395 2213 116-117 0.4886718776819272 2213 118-119 0.5294459030669607 2213 120-121 0.518040014834142 2213 122-123 0.7769190177312453 2213 124-125 0.6111401119698492 2214 1 -0.1491981136616367 2214 2 -0.11748847207634583 2214 3 -0.21387027169950557 2214 4 -5.311395464820386 2214 5 -1.5035013730270386 2214 6 -0.9964697823505944 2214 7 -0.6129762874131615 2214 8 -0.20166146065770718 2214 9 0.1530403220798604 2214 10-11 0.0683922757893356 2214 12-13 -0.11175812658476403 2214 14-15 -0.10580455478506678 2214 16-17 -0.33494212207278196 2214 18-19 0.2538473581679135 2214 20-21 0.5367466635137319 2214 22-23 0.6664308976740472 2214 24-25 0.37300604240231294 2214 26-27 0.24379805150411826 2214 28-29 0.3244992646539302 2214 30-31 0.3644571560288341 2214 32-33 0.20105040459121426 2214 34-35 0.475343143542748 2214 36-37 0.6719973886133062 2214 38-39 0.6518758383622263 2214 40-41 0.43911547615151747 2214 42-43 0.4529827844727663 2214 44-45 0.6415965022911436 2214 46-47 0.7725489624661463 2214 48-49 0.8469727522011645 2214 50-51 0.6809124787719085 2214 52-53 0.6767774651133642 2214 54-55 0.5686608902777941 2214 56-57 0.6475624364555692 2214 58-59 0.4451261304343781 2214 60-61 0.843597305245865 2214 62-63 0.5349056839740527 2214 64-65 0.30213596701026546 2214 66-67 0.4392049321622409 2214 68-69 0.3111737809710782 2214 70-71 0.13451283158261518 2214 72-73 0.060938156606923144 2214 74-75 0.22307511538877378 2214 76-77 0.20196188417473593 2214 78-79 -2.696681996685598 2214 80-81 -0.8661952824501071 2214 82-83 -0.3023966352559988 2214 84-85 -2.391575522573973 2214 86-87 -0.7662500342478928 2214 88-89 -0.3514227692476304 2214 90-91 -1.9530165834468995 2214 92-93 -1.3007872713125366 2214 94-95 -0.32522362714610864 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26-27 -0.23640317088045038 2215 28-29 -0.6098145312852914 2215 30-31 -0.25990087916029836 2215 32-33 -0.48237913863138004 2215 34-35 -0.006375580259014413 2215 36-37 -0.12645766420233073 2215 38-39 -0.20974902424259412 2215 40-41 -0.6135569431479482 2215 42-43 -0.5490215216849315 2215 44-45 -0.28794249531625127 2215 46-47 -0.37755642927976396 2215 48-49 -0.3433864313854009 2215 50-51 -1.2510878885023544 2215 52-53 -0.8692109284875507 2215 54-55 -0.7355304942131191 2215 56-57 -0.5373813812273518 2215 58-59 -0.4134006872405642 2215 60-61 -0.3197339521894449 2215 62-63 -0.3427996702460092 2215 64-65 -0.26554070388804263 2215 66-67 -0.382624466279772 2215 68-69 -0.5244605722136946 2215 70-71 -0.3527993395671949 2215 72-73 -0.329056946402865 2215 74-75 -0.5828344073942091 2215 76-77 -0.5054335358087094 2215 78-79 -2.624392074904371 2215 80-81 -2.5084112969388066 2215 82-83 -1.5522485279161025 2215 84-85 -4.395672659963825 2215 86-87 -2.217501927174137 2215 88-89 -3.4957786553500263 2215 90-91 -5.437473496586538 2215 92-93 -4.278884517820103 2215 94-95 -3.0019026760122145 2215 96-97 -1.6857757946979355 2215 98-99 -1.492578251648247 2215 100-101 -1.476920689561144 2215 102-103 -1.428934108600405 2215 104-105 -1.6331496134986203 2215 106-107 -1.5932114434111462 2215 108-109 -3.701369750159401 2215 110-111 -3.0034706600845205 2215 112-113 -1.9268091083433703 2215 114-115 -1.689448780139756 2215 116-117 -1.5937101173230062 2215 118-119 -1.6820241752596985 2215 120-121 -1.6092371119109075 2215 122-123 -1.4715231469542047 2215 124-125 -1.7322763809027997 2216 1 -3.106678317761272 2216 2 -3.1643896022690257 2216 3 -4.516678168346925 2216 4 -4.47773372649041 2216 5 -4.215691203328507 2216 6 -3.327843937872526 2216 7 -3.2457161395404412 2216 8 -3.1234063609940286 2216 9 -3.6746149634990424 2216 10-11 -9.476949920181596 2216 12-13 -4.893356590409322 2216 14-15 -4.316582492392641 2216 16-17 -4.16641734920826 2216 18-19 -3.74805595460618 2216 20-21 -3.3204676162230946 2216 22-23 -3.6851287433422044 2216 24-25 -3.990352599828487 2216 26-27 -3.860273395807461 2216 28-29 -4.1837169669665855 2216 30-31 -3.8658107530129335 2216 32-33 -4.234031481792421 2216 34-35 -3.9978212263245076 2216 36-37 -3.6315949368423794 2216 38-39 -4.02133164596955 2216 40-41 -4.044162642395449 2216 42-43 -4.1693905975332655 2216 44-45 -3.953398403134095 2216 46-47 -4.091914201666238 2216 48-49 -3.921864621734965 2216 50-51 -3.902488028310735 2216 52-53 -4.415434959994695 2216 54-55 -4.812369819660528 2216 56-57 -4.014644243826783 2216 58-59 -4.808930474192696 2216 60-61 -4.4414535952668714 2216 62-63 -4.687128521487153 2216 64-65 -4.510123129152241 2216 66-67 -5.275528962535557 2216 68-69 -5.038310739663931 2216 70-71 -6.883008915070576 2216 72-73 -7.308416477816344 2216 74-75 -5.331260192445676 2216 76-77 -4.862754057942752 2216 78-79 -5.173632495060538 2216 80-81 -5.089042810478745 2216 82-83 -5.33425847898317 2216 84-85 -5.076700297247889 2216 86-87 -5.2456769709213695 2216 88-89 -5.493081632903017 2216 90-91 -5.471534655899557 2216 92-93 -5.241525068646844 2216 94-95 -8.333180917533479 2216 96-97 -9.991325639126847 2216 98-99 -7.074035822546172 2216 100-101 -6.174304993557563 2216 102-103 -6.993918505802412 2216 104-105 -9.546967798639638 2216 106-107 -6.476215130514511 2216 108-109 -8.643639854467416 2216 110-111 -6.249223637865953 2216 112-113 -5.699362347465957 2216 114-115 -6.058599558452094 2216 116-117 -5.512064764580984 2216 118-119 -5.849430090159345 2216 120-121 -6.52702201717474 2216 122-123 -9.318887576229894 2216 124-125 -7.9374680234685115 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 286530.0 3 41487.0 4 24394.0 5 20934.0 6 20824.0 7 22555.0 8 24270.0 9 26107.0 10 27365.0 11 28183.0 12 28202.0 13 28962.0 14 29525.0 15 24774.0 16 20224.0 17 18008.0 18 16738.0 19 15577.0 20 15560.0 21 16963.0 22 21330.0 23 29077.0 24 39461.0 25 48523.0 26 57679.0 27 64393.0 28 72336.0 29 81924.0 30 90342.0 31 101665.0 32 118606.0 33 141547.0 34 171396.0 35 212345.0 36 263862.0 37 255908.0 38 64749.0 39 86.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57152836069255 19.47783030010106 13.89819282476833 23.052448514438062 2 14.926157281471092 20.92573962299517 40.64618066101259 23.501922434521145 3 17.991246131192913 27.844521209113545 28.78870848932369 25.375524170369857 4 11.046960052741817 17.21386300741147 38.478810897641594 33.26036604220512 5 12.713627923150348 39.95943899162253 33.72736530034516 13.59956778488196 6 30.150892666928947 38.502316222264035 17.597457831381508 13.749333279425507 7 27.945027399220006 33.90933377288193 21.081071835094637 17.064566992803428 8 26.089552726199766 35.64129148934599 19.998818186094482 18.270337598359767 9 26.817439268366833 17.035375695217123 20.47143749091251 35.675747545503526 10-11 24.0470115675359 28.182978446969713 27.146249176789933 20.623760808704457 12-13 25.048508096604884 25.648391233981442 27.669383742099818 21.63371692731386 14-15 22.461592472666997 26.950205851633253 25.37152725602368 25.216674419676067 16-17 21.900067519056684 30.366234017201126 25.780284474330216 21.95341398941197 18-19 21.803678400642628 28.69621798079499 28.11486535613486 21.38523826242752 20-21 22.784094669028203 27.74454543685163 28.18622394363456 21.285135950485607 22-23 22.58040999904355 27.74490028405844 27.875887536809792 21.798802180088227 24-25 22.18892361741562 27.881682557592047 28.077560261165086 21.851833563827245 26-27 21.79755628479713 28.247754575697126 27.890418862069545 22.0642702774362 28-29 21.99115069190253 27.932798440612196 28.095027332633176 21.981023534852095 30-31 22.025534942168807 27.746940306703095 28.07906754795941 22.14845720316869 32-33 21.71936765039763 28.131599285916444 27.816699633938875 22.332333429747052 34-35 22.143259156005684 27.908658168983553 27.945771134122833 22.00231154088793 36-37 22.492240988379606 27.333354973882646 27.769574022632774 22.404830015104974 38-39 22.089235416581623 27.793160289792528 27.935723907883762 22.181880385742083 40-41 22.036231506462578 27.71351320242 28.216298655998234 22.033956635119193 42-43 22.104698588815694 27.714327759525005 27.538631979547905 22.642341672111396 44-45 21.914785391180892 27.816912234300283 27.770871951582592 22.497430422936233 46-47 22.304891171663733 27.839920413885118 27.496124103334434 22.359064311116708 48-49 22.2435359624657 27.989012321968282 27.224322493909774 22.543129221656248 50-51 22.41294514939851 28.025138077083678 27.004285191091842 22.557631582425973 52-53 22.47928833276765 27.92841338656608 27.12984443456925 22.462453846097024 54-55 22.331564640237872 27.925730441219464 27.098210675607586 22.644494242935075 56-57 22.26511831241023 28.134640807717332 26.897507285974676 22.70273359389776 58-59 22.250472828841307 27.677006336849313 27.167037419428585 22.9054834148808 60-61 22.275212147655235 28.083655900430788 27.281554606195936 22.359577345718044 62-63 22.135855144864856 28.163729844784186 27.41177385337597 22.288641156974993 64-65 22.071682603563914 27.792695670834778 27.243677465605693 22.891944259995615 66-67 22.100053981416163 28.202208903220864 26.881559485596174 22.816177629766795 68-69 22.182411664493415 27.95531187139946 27.284422330923707 22.577854133183422 70-71 22.186635617748422 27.488069945593118 27.630647459360063 22.694646977298394 72-73 22.08328474020168 28.03800371640271 26.944502756202425 22.93420878719318 74-75 22.007053785845972 28.269334496977987 27.04375621385275 22.67985550332329 76-77 22.10811286795715 27.848529162778124 27.464556974082218 22.57880099518251 78-79 22.009616911891506 27.802257814348458 27.359789658971543 22.828335614788493 80-81 22.16653322689252 28.460225520021503 26.7258388968875 22.647402356198477 82-83 22.466559861414936 27.911839404917732 26.93708273934089 22.68451799432644 84-85 21.996100035337598 28.010333817848483 26.946998656082748 23.04656749073117 86-87 22.146494784843195 28.04626969533221 27.1285148368587 22.678720682965896 88-89 22.145028806441974 27.94941169312757 27.023647932193334 22.881911568237122 90-91 22.008634770511467 28.000802402698465 27.21085949419473 22.779703332595336 92-93 22.268836019171808 27.963969476569584 26.945277782421233 22.821916721837376 94-95 21.9390836402088 27.450071385599838 27.370998105778977 23.239846868412382 96-97 22.235269054800714 27.439892674306094 27.285773319955204 23.03906495093799 98-99 21.899746046123777 27.639356339176636 27.13537500716766 23.325522607531923 100-101 21.82846504351896 27.642344849624482 27.33791232607674 23.191277780779814 102-103 22.11546871871823 27.193596603929816 27.50624420528031 23.184690472071637 104-105 21.679753558496813 27.631814248293544 27.364134892823294 23.32429730038635 106-107 21.752902942399867 27.491126928653898 27.47363346926399 23.28233665968224 108-109 21.950037630579665 27.354768509735745 27.126108313870134 23.569085545814453 110-111 21.586811082432096 27.713291567235533 27.284052292434737 23.415845057897638 112-113 21.525451304273286 27.774657995031284 27.675602463092773 23.024288237602654 114-115 21.445326607418387 27.96243030176038 27.273407587413633 23.3188355034076 116-117 21.301551782318175 27.8890263378466 27.582584707347756 23.226837172487468 118-119 21.38701908592066 27.62908119728727 27.333996808322464 23.64990290846961 120-121 21.230317361736788 27.940098494589794 27.3848976906158 23.444686453057617 122-123 21.314641561674314 27.923712348974583 27.449776765104872 23.31186932424623 124-125 21.46788020205018 27.915235853891108 27.08159313373232 23.535290810326394 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2609.0 1 2637.0 2 2762.0 3 2406.0 4 1689.0 5 1287.0 6 1134.5 7 1273.5 8 1623.0 9 1803.0 10 1712.0 11 1604.0 12 1635.0 13 1771.0 14 1914.0 15 2094.5 16 2283.5 17 2470.5 18 2685.0 19 3015.5 20 3454.0 21 4169.5 22 5114.5 23 6155.5 24 7314.0 25 9190.0 26 12113.0 27 15554.0 28 19060.0 29 23801.0 30 30671.5 31 38131.5 32 47070.5 33 54968.5 34 60967.0 35 67485.0 36 76783.0 37 84907.5 38 86775.5 39 87039.5 40 85745.5 41 84064.5 42 81829.0 43 82828.0 44 86728.5 45 88581.5 46 86954.0 47 83881.0 48 84335.5 49 83756.0 50 81123.5 51 78413.0 52 76888.0 53 77267.5 54 76831.0 55 73376.5 56 68593.5 57 63991.0 58 60157.0 59 54446.0 60 46285.5 61 39092.5 62 32798.0 63 25804.0 64 19824.5 65 15829.5 66 12370.5 67 9872.5 68 7603.0 69 5494.0 70 4067.5 71 3008.5 72 2397.0 73 2051.0 74 1681.5 75 1318.5 76 962.5 77 659.0 78 435.0 79 310.5 80 228.5 81 160.5 82 115.5 83 86.5 84 66.5 85 52.0 86 44.0 87 34.5 88 26.5 89 27.0 90 23.0 91 17.5 92 9.0 93 4.0 94 5.0 95 7.5 96 6.5 97 3.0 98 1.5 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10534863985576176 2 0.036230602341538735 3 0.046843214400809206 4 0.05426815543861382 5 0.05710596012845537 6 0.0046260103848102035 7 0.0029155527635358423 8 0.003654159463631589 9 0.007502689111498902 10-11 0.06538612997689716 12-13 0.006219845895543131 14-15 0.011331781740942641 16-17 0.021166913063270214 18-19 0.018581789612935103 20-21 0.13454304152796734 22-23 0.01574398492309355 24-25 0.015471866665163538 26-27 0.006997326632486023 28-29 0.004645447403233776 30-31 0.002079760971322234 32-33 0.006336468006084565 34-35 0.020739298657951627 36-37 0.003090485929347993 38-39 0.008785532327454671 40-41 0.03253756884106 42-43 0.013664223951771314 44-45 0.11358993566735642 46-47 0.00400402579525589 48-49 0.009990627469716153 50-51 0.011545588943601935 52-53 0.012050951422614815 54-55 0.0103793678381876 56-57 0.006783519429826727 58-59 0.015219185425657098 60-61 0.03315955343061431 62-63 0.00723057085356889 64-65 0.007113948743027456 66-67 0.008921591456419678 68-69 0.008902154437996105 70-71 0.004898128642740215 72-73 0.0012050951422614815 74-75 0.001302280234379343 76-77 0.008532851087948231 78-79 0.02283849664769743 80-81 0.0034792262978194386 82-83 0.004975876716434504 84-85 0.0031099229477715654 86-87 0.003926277721561602 88-89 0.006083786766578125 90-91 0.005306306029635234 92-93 0.0035764113899372997 94-95 0.004412203182150908 96-97 0.0020991979897458067 98-99 0.0036347224452080167 100-101 0.0032654190951601435 102-103 0.0010107249580257587 104-105 0.004548262311115914 106-107 8.163547737900359E-4 108-109 0.00136059128965006 110-111 0.0027017455608765472 112-113 0.004528825292692342 114-115 0.002798930652994409 116-117 0.005558987269141673 118-119 0.0037319075373258783 120-121 0.007327755945686751 122-123 0.0046843214400809205 124-125 0.056969900999490355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2572411.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.508813415460665 #Duplication Level Percentage of deduplicated Percentage of total 1 80.4686817488052 44.667210409825124 2 9.541835375348425 10.593119189825154 3 3.813680125267413 6.350785754991585 4 1.951465077023522 4.332940433891451 5 1.0932847316036916 3.034346908828065 6 0.7183251370586791 2.392402560277525 7 0.45885350289514937 1.7829289434066167 8 0.32429942852130594 1.4401181174823756 9 0.23852292949962145 1.1916112310013207 >10 1.2303816699114594 12.21992799031756 >50 0.09649726712667935 3.723139665310575 >100 0.059299632780351406 6.050871806194196 >500 0.004273574262009609 1.5730667949275017 >1k 5.997998964219602E-4 0.6475301937209086 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3637 0.1413848720130648 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3254 0.1264961159006084 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.8874036847144565E-5 0.0 0.0 0.0 5 0.0 3.8874036847144565E-5 0.0 0.0 0.0 6 0.0 3.8874036847144565E-5 0.0 0.0 0.0 7 0.0 3.8874036847144565E-5 0.0 0.0 0.0 8 0.0 3.8874036847144565E-5 0.0 0.0 0.0 9 0.0 3.8874036847144565E-5 0.0 0.0 0.0 10-11 0.0 3.8874036847144565E-5 0.0 0.0 0.0 12-13 0.0 3.8874036847144565E-5 0.0 3.8874036847144565E-5 0.0 14-15 0.0 3.8874036847144565E-5 0.0 3.8874036847144565E-5 0.0 16-17 0.0 3.8874036847144565E-5 0.0 5.831105527071685E-5 0.0 18-19 0.0 3.8874036847144565E-5 0.0 9.718509211786142E-5 0.0 20-21 0.0 3.8874036847144565E-5 0.0 1.9437018423572284E-4 0.0 22-23 0.0 3.8874036847144565E-5 0.0 4.0817738689501794E-4 0.0 24-25 0.0 3.8874036847144565E-5 0.0 9.912879396021864E-4 0.0 26-27 0.0 3.8874036847144565E-5 0.0 0.0016132725291564994 3.8874036847144565E-5 28-29 0.0 3.8874036847144565E-5 0.0 0.0028766787266886976 7.774807369428913E-5 30-31 0.0 3.8874036847144565E-5 0.0 0.006414216079778854 7.774807369428913E-5 32-33 0.0 3.8874036847144565E-5 0.0 0.013469853767535593 7.774807369428913E-5 34-35 0.0 3.8874036847144565E-5 0.0 0.022508067334496704 7.774807369428913E-5 36-37 0.0 3.8874036847144565E-5 0.0 0.037066394133752345 7.774807369428913E-5 38-39 0.0 3.8874036847144565E-5 0.0 0.06295650267395062 7.774807369428913E-5 40-41 0.0 3.8874036847144565E-5 0.0 0.0948137758701856 7.774807369428913E-5 42-43 0.0 3.8874036847144565E-5 0.0 0.12507721355568763 7.774807369428913E-5 44-45 0.0 3.8874036847144565E-5 0.0 0.15940298809171627 7.774807369428913E-5 46-47 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.20278641321312962 7.774807369428913E-5 48-49 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.25170938858526104 7.774807369428913E-5 50-51 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.30922352610061143 7.774807369428913E-5 52-53 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.36860361738462477 7.774807369428913E-5 54-55 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.4302772768426196 7.774807369428913E-5 56-57 7.774807369428913E-5 3.8874036847144565E-5 0.0 0.5013778902360471 7.774807369428913E-5 58-59 7.774807369428913E-5 3.8874036847144565E-5 0.0 0.5844905810152421 7.774807369428913E-5 60-61 1.166221105414337E-4 3.8874036847144565E-5 0.0 0.6792849198670041 9.718509211786142E-5 62-63 1.166221105414337E-4 3.8874036847144565E-5 0.0 0.7760618345979706 1.166221105414337E-4 64-65 1.166221105414337E-4 3.8874036847144565E-5 0.0 0.87688164916104 1.166221105414337E-4 66-67 1.166221105414337E-4 3.8874036847144565E-5 0.0 0.9931150193340023 1.166221105414337E-4 68-69 1.166221105414337E-4 3.8874036847144565E-5 0.0 1.1290575261884668 1.166221105414337E-4 70-71 1.166221105414337E-4 3.8874036847144565E-5 0.0 1.2868861157878737 1.166221105414337E-4 72-73 1.166221105414337E-4 3.8874036847144565E-5 0.0 1.4545692737280318 1.166221105414337E-4 74-75 1.166221105414337E-4 3.8874036847144565E-5 0.0 1.6198228043652434 1.166221105414337E-4 76-77 1.166221105414337E-4 3.8874036847144565E-5 0.0 1.799615224783287 1.166221105414337E-4 78-79 1.166221105414337E-4 7.774807369428913E-5 0.0 2.012333954410862 1.166221105414337E-4 80-81 1.166221105414337E-4 7.774807369428913E-5 0.0 2.2568516461794017 1.166221105414337E-4 82-83 1.166221105414337E-4 7.774807369428913E-5 0.0 2.5220891995874686 1.166221105414337E-4 84-85 1.166221105414337E-4 7.774807369428913E-5 0.0 2.7846833184899302 1.166221105414337E-4 86-87 1.166221105414337E-4 7.774807369428913E-5 0.0 3.050134679100657 1.166221105414337E-4 88-89 1.166221105414337E-4 7.774807369428913E-5 0.0 3.3428756135780793 1.166221105414337E-4 90-91 1.166221105414337E-4 7.774807369428913E-5 0.0 3.692158834649673 1.166221105414337E-4 92-93 1.166221105414337E-4 7.774807369428913E-5 1.9437018423572283E-5 4.070714205467167 1.166221105414337E-4 94-95 1.166221105414337E-4 7.774807369428913E-5 3.8874036847144565E-5 4.4447407509919685 1.166221105414337E-4 96-97 1.166221105414337E-4 7.774807369428913E-5 3.8874036847144565E-5 4.818184185964062 1.166221105414337E-4 98-99 1.166221105414337E-4 7.774807369428913E-5 3.8874036847144565E-5 5.215301909376068 1.166221105414337E-4 100-101 1.3605912896500597E-4 7.774807369428913E-5 3.8874036847144565E-5 5.673646240822325 1.166221105414337E-4 102-103 1.5549614738857826E-4 7.774807369428913E-5 3.8874036847144565E-5 6.180855236585445 1.166221105414337E-4 104-105 1.5549614738857826E-4 7.774807369428913E-5 3.8874036847144565E-5 6.684623880087591 1.166221105414337E-4 106-107 1.5549614738857826E-4 7.774807369428913E-5 3.8874036847144565E-5 7.182250425767888 1.166221105414337E-4 108-109 1.5549614738857826E-4 7.774807369428913E-5 3.8874036847144565E-5 7.691189316170705 1.166221105414337E-4 110-111 1.5549614738857826E-4 7.774807369428913E-5 3.8874036847144565E-5 8.268624259498191 1.166221105414337E-4 112-113 1.5549614738857826E-4 7.774807369428913E-5 3.8874036847144565E-5 8.889326005836548 1.166221105414337E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8550 0.0 50.2012 1 GGTATCA 4800 0.0 46.756832 1 TCAACGC 10395 0.0 39.78347 4 ATCAACG 10420 0.0 39.63244 3 CAACGCA 10430 0.0 39.592064 5 GTACATG 14110 0.0 38.857742 1 AACGCAG 10455 0.0 38.39131 6 TATCAAC 11515 0.0 37.51731 2 TACATGG 14965 0.0 35.468857 2 ACATGGG 14805 0.0 33.078854 3 ACGCAGA 12015 0.0 32.61427 7 CGCAGAG 11875 0.0 31.997019 8 CATGGGA 9145 0.0 28.670593 4 GCAGAGT 12370 0.0 28.646935 9 CATGGGG 5585 0.0 27.31395 4 GAGTACT 4965 0.0 25.038767 12-13 ATGGGAT 4190 0.0 24.7454 5 GAGTACA 10610 0.0 24.463356 1 CATGGGT 2125 0.0 23.555267 4 ATGGGAG 3525 0.0 23.32809 5 >>END_MODULE