FastQCFastQC Report
Thu 26 May 2016
SRR1364505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572411
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101980.3964374277671803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90590.3521598997982826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58010.2255082877502856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71550.064.041941
GTATCAA127550.049.1626321
ATCAACG167400.035.957373
TATCAAC175400.035.5405462
TCAACGC168200.035.502894
CAACGCA168450.035.2856455
AACGCAG171400.034.1229256
GTACATG141150.032.928311
ACGCATA2151.2187229E-1030.4415117
TACATGG151750.030.0830652
ACATGGG149200.028.4801793
CGCAGAG205850.028.4579228
ACGCAGA203650.028.3983977
ATATAGG12400.027.3952432
GAGTACT128950.026.68241512-13
GCAGAGT212500.026.6157919
CATGGGA93000.026.1456364
TAAGGTG12100.025.5694565
GTACTTT138800.025.1745414-15
CTTACTA15550.024.477529
Produced by FastQC (version 0.11.5)