##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364505_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2572411 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.153457204155945 31.0 28.0 33.0 25.0 34.0 2 29.31043950597319 31.0 28.0 33.0 25.0 34.0 3 29.379203401011736 31.0 28.0 34.0 25.0 34.0 4 32.83777864423686 35.0 32.0 37.0 27.0 37.0 5 32.033622931949836 35.0 32.0 37.0 22.0 37.0 6 32.105279832810545 35.0 32.0 37.0 23.0 37.0 7 31.535711050839076 35.0 32.0 37.0 18.0 37.0 8 31.661856134186955 35.0 32.0 37.0 18.0 37.0 9 32.22495938635 37.0 31.0 39.0 15.0 39.0 10-11 32.435400486158706 37.0 31.5 39.0 17.0 39.0 12-13 32.202285715618544 37.0 31.0 39.0 15.0 39.0 14-15 33.14433793822216 38.0 31.5 40.0 16.5 41.0 16-17 33.23752114261679 38.0 32.0 40.0 17.0 41.0 18-19 33.14594907267929 38.0 31.5 40.0 16.5 41.0 20-21 33.05092829256289 38.0 31.5 40.0 14.0 41.0 22-23 33.00044471898153 38.0 31.0 40.0 14.5 41.0 24-25 32.84970344940991 38.0 31.0 40.0 12.5 41.0 26-27 32.88856485219508 38.0 31.0 40.0 14.0 41.0 28-29 32.745580119195566 37.5 31.0 40.0 10.5 41.0 30-31 32.18994515262141 37.0 30.0 40.0 9.0 41.0 32-33 32.083812617812626 37.0 30.0 40.0 9.0 41.0 34-35 31.92081747434605 37.0 30.0 40.0 8.0 41.0 36-37 31.83509711317515 37.0 30.0 40.0 7.5 41.0 38-39 31.608049413565716 36.5 30.0 40.0 2.0 41.0 40-41 31.265582560485086 36.0 29.5 40.0 2.0 41.0 42-43 31.133159903296946 36.0 29.5 40.0 2.0 41.0 44-45 31.009493234168257 36.0 29.0 40.0 2.0 41.0 46-47 30.770623162472873 36.0 28.5 40.0 2.0 41.0 48-49 30.42957249055458 36.0 28.0 40.0 2.0 41.0 50-51 30.103177719268032 35.0 27.5 40.0 2.0 41.0 52-53 29.977856765501315 35.0 27.0 40.0 2.0 41.0 54-55 29.501208010695024 35.0 26.0 39.5 2.0 41.0 56-57 29.512561367526416 35.0 26.0 39.0 2.0 41.0 58-59 29.29391318105855 35.0 26.0 39.0 2.0 41.0 60-61 29.022811284821906 35.0 26.0 39.0 2.0 41.0 62-63 28.750134212612217 34.0 26.0 39.0 2.0 40.5 64-65 28.256445218124163 34.0 24.5 38.0 2.0 40.0 66-67 27.901327198491998 34.0 23.0 38.0 2.0 40.0 68-69 27.700033159553428 34.0 23.0 37.0 2.0 40.0 70-71 27.764701480439946 34.0 24.5 37.0 2.0 39.5 72-73 27.566193738092398 34.0 24.5 36.5 2.0 39.0 74-75 27.191683210808847 34.0 23.5 36.0 2.0 39.0 76-77 27.09295404194742 33.5 24.0 36.0 2.0 39.0 78-79 26.705590397490916 33.0 23.0 35.0 2.0 37.5 80-81 26.49409231262034 33.0 22.5 35.0 2.0 37.0 82-83 26.342156832636775 33.0 23.0 35.0 2.0 36.5 84-85 25.923331458308954 33.0 20.0 35.0 2.0 36.0 86-87 25.81141757674026 33.0 20.0 35.0 2.0 36.0 88-89 25.744944334322938 33.0 20.0 35.0 2.0 36.0 90-91 25.579434623782902 33.0 20.0 35.0 2.0 35.0 92-93 25.608445151260824 33.0 20.0 35.0 2.0 35.0 94-95 25.592273551932408 33.0 20.0 35.0 2.0 35.0 96-97 25.473965863153282 33.0 20.0 35.0 2.0 35.0 98-99 25.36109004354281 33.0 19.5 35.0 2.0 35.0 100-101 25.281089608153597 33.0 19.0 35.0 2.0 35.0 102-103 24.108381592210577 30.5 17.5 34.0 2.0 35.0 104-105 25.056666489141897 32.0 19.0 35.0 2.0 35.0 106-107 25.188961833859363 33.0 19.0 35.0 2.0 35.0 108-109 25.242910055974725 33.0 19.0 35.0 2.0 35.0 110-111 25.172971581912844 33.0 18.0 35.0 2.0 35.0 112-113 25.109216606522054 33.0 18.0 35.0 2.0 35.0 114-115 25.04805472375915 33.0 17.0 35.0 2.0 35.0 116-117 24.964413734819203 33.0 14.0 35.0 2.0 35.0 118-119 24.844784717527645 33.0 6.5 35.0 2.0 35.0 120-121 24.678970623279096 33.0 2.0 35.0 2.0 35.0 122-123 24.386460600580545 33.0 2.0 35.0 2.0 35.0 124-125 23.602210921971643 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8470569831325108 1101 2 0.8440422956802287 1101 3 0.8230636802055251 1101 4 0.8246760305774501 1101 5 1.0019612088459553 1101 6 1.1156417959420253 1101 7 1.5398257542295042 1101 8 1.2903967673404964 1101 9 2.0141725904255665 1101 10-11 1.5832129579123873 1101 12-13 1.890381676436121 1101 14-15 1.773509602597258 1101 16-17 1.638444459695208 1101 18-19 1.7534146093680434 1101 20-21 2.0096354947949635 1101 22-23 1.9370171334934057 1101 24-25 2.088030933639459 1101 26-27 1.8943625415062257 1101 28-29 1.963000562488773 1101 30-31 2.450063562520519 1101 32-33 2.2868567205794434 1101 34-35 2.241907704007229 1101 36-37 2.1379000110472184 1101 38-39 2.2167863729743615 1101 40-41 2.3356421198074777 1101 42-43 2.2557928855518306 1101 44-45 2.149560984818063 1101 46-47 1.9548191382296167 1101 48-49 2.017088530101592 1101 50-51 2.1852421321306394 1101 52-53 2.022272083518601 1101 54-55 2.3862759983673847 1101 56-57 2.1318232078029027 1101 58-59 2.253752634638314 1101 60-61 2.387573133644292 1101 62-63 2.262970646140829 1101 64-65 2.4238235031339777 1101 66-67 2.5850213007383616 1101 68-69 2.5327065650878815 1101 70-71 2.129307954977719 1101 72-73 2.2937885897421815 1101 74-75 2.3806843987393087 1101 76-77 2.153016908400879 1101 78-79 2.20334497487136 1101 80-81 2.350887916959522 1101 82-83 2.1888710465510712 1101 84-85 2.4802628297863833 1101 86-87 2.2920577580526 1101 88-89 2.1910898741757023 1101 90-91 2.109553699004156 1101 92-93 2.01766770187335 1101 94-95 1.9915194936130192 1101 96-97 1.8992780756172323 1101 98-99 1.9707029136455425 1101 100-101 1.9976638322387785 1101 102-103 1.8788531303218683 1101 104-105 1.9621965088876188 1101 106-107 1.9679819230090096 1101 108-109 1.9467951727214832 1101 110-111 1.8868058900470537 1101 112-113 1.8065088925229418 1101 114-115 1.7272646695385347 1101 116-117 1.8061003314398079 1101 118-119 1.753355657778858 1101 120-121 1.7070575971429562 1101 122-123 1.8081312539997612 1101 124-125 1.715300536154949 1102 1 0.3952147491284279 1102 2 -1.7346489926767177 1102 3 -0.16425426272179422 1102 4 0.34768219987191173 1102 5 0.9553042894980877 1102 6 0.5894855965455505 1102 7 1.239188849252919 1102 8 0.8882769567011195 1102 9 1.8524420066869425 1102 10-11 1.394551568114892 1102 12-13 1.4930219606065265 1102 14-15 1.4799730737340084 1102 16-17 1.4408823066659977 1102 18-19 1.592914370309039 1102 20-21 1.6775584003467685 1102 22-23 1.6487919685796868 1102 24-25 1.7949573561792533 1102 26-27 1.586301767805864 1102 28-29 1.6415948400543101 1102 30-31 2.0546263989717843 1102 32-33 1.6640429082402974 1102 34-35 1.9579253971479424 1102 36-37 1.3942982380339721 1102 38-39 1.3061383982249097 1102 40-41 1.4471111258306486 1102 42-43 1.3680618375670939 1102 44-45 1.2830382159506648 1102 46-47 0.8652021597689696 1102 48-49 0.8721319352635994 1102 50-51 1.212826212003165 1102 52-53 0.9737082408924849 1102 54-55 1.339415069671702 1102 56-57 0.9876955974589094 1102 58-59 0.8975010798299898 1102 60-61 0.8338067752111478 1102 62-63 1.1995304899617771 1102 64-65 1.1609269403123186 1102 66-67 1.382349573604099 1102 68-69 1.4280513418104697 1102 70-71 0.7802874495413228 1102 72-73 1.1037461139971931 1102 74-75 1.333553245513123 1102 76-77 1.0883062715236456 1102 78-79 0.9209314869496197 1102 80-81 0.9486270753793455 1102 82-83 0.648044669973487 1102 84-85 0.7271608426835456 1102 86-87 0.8366165993978782 1102 88-89 0.8847569838182423 1102 90-91 0.6521586375840265 1102 92-93 0.6779743817460613 1102 94-95 0.6744514205210734 1102 96-97 0.7299251625653511 1102 98-99 0.6476948022856632 1102 100-101 0.46264571091887774 1102 102-103 0.23272861433543568 1102 104-105 0.5247499734860774 1102 106-107 0.7741676787915104 1102 108-109 0.7671036281968604 1102 110-111 0.7598363351592035 1102 112-113 0.7852377274778988 1102 114-115 1.0382320678089876 1102 116-117 0.5405988115424236 1102 118-119 0.15057230812292488 1102 120-121 0.4293137592702756 1102 122-123 0.8142126931257216 1102 124-125 0.867685068806157 1103 1 0.7178715423613689 1103 2 0.625248774815212 1103 3 0.6230266336445887 1103 4 0.5556833757482806 1103 5 0.8843627353405736 1103 6 0.7569180557657731 1103 7 1.056664483265692 1103 8 0.9956526350854347 1103 9 1.5746037780624533 1103 10-11 1.3539383571807164 1103 12-13 1.2902027417787565 1103 14-15 1.1436881127386158 1103 16-17 1.1897610508594951 1103 18-19 1.3626040702825932 1103 20-21 1.5181066189493038 1103 22-23 1.3461533965207408 1103 24-25 1.6674275189301184 1103 26-27 1.2677018401013953 1103 28-29 1.3546652950558666 1103 30-31 1.9262295983865485 1103 32-33 1.7627860829714805 1103 34-35 1.8169675857018142 1103 36-37 1.7768696758987907 1103 38-39 1.7474644688646421 1103 40-41 1.9146025322978524 1103 42-43 1.8849268566566728 1103 44-45 1.8434869146617991 1103 46-47 1.7798301599874549 1103 48-49 1.8242526552976166 1103 50-51 2.0096155969572322 1103 52-53 1.8232917744500696 1103 54-55 2.055314532242196 1103 56-57 1.8746103212599081 1103 58-59 1.9427187734552795 1103 60-61 1.9128513511131722 1103 62-63 1.9088513968103982 1103 64-65 2.0582288379779357 1103 66-67 2.3497469897446592 1103 68-69 2.334549132746279 1103 70-71 2.1303674471846605 1103 72-73 2.2502901973935856 1103 74-75 2.3084939146947896 1103 76-77 2.127905626632785 1103 78-79 2.229667432127762 1103 80-81 2.1840080353878903 1103 82-83 2.099117227559823 1103 84-85 2.304944358688907 1103 86-87 2.1792524588401463 1103 88-89 2.159200022857217 1103 90-91 2.236409811158964 1103 92-93 1.9919368703079634 1103 94-95 1.9255320177631674 1103 96-97 1.7944610135997365 1103 98-99 1.9944110018237815 1103 100-101 1.9467186925817117 1103 102-103 1.8175259935303991 1103 104-105 1.8485012339015725 1103 106-107 1.8299429747091445 1103 108-109 1.7983988167390628 1103 110-111 1.8216846704288052 1103 112-113 1.9203218953090868 1103 114-115 1.7433138973891502 1103 116-117 1.818813339016259 1103 118-119 1.9107799092185296 1103 120-121 1.8625390789079646 1103 122-123 2.031425166498199 1103 124-125 1.968222099399192 1104 1 0.5813893687202949 1104 2 0.5502650598504708 1104 3 0.5593579441642262 1104 4 0.6641637386589423 1104 5 0.8398142914836484 1104 6 0.8299806668749383 1104 7 1.2456141325823609 1104 8 1.0354023402997825 1104 9 1.528038425741002 1104 10-11 1.2085409353037235 1104 12-13 1.2111419113299604 1104 14-15 1.181756546133954 1104 16-17 1.0716932048792742 1104 18-19 1.0422572925633844 1104 20-21 1.3650149913979988 1104 22-23 1.37918363802644 1104 24-25 1.6032731338820696 1104 26-27 1.4325339903099703 1104 28-29 1.465685470783896 1104 30-31 1.9896609325310024 1104 32-33 1.846887611148233 1104 34-35 1.7478820896454756 1104 36-37 1.644208206379627 1104 38-39 1.721648127931708 1104 40-41 1.866666224180907 1104 42-43 1.7512725215505647 1104 44-45 1.4480036823914553 1104 46-47 1.466219658403702 1104 48-49 1.2311337222672556 1104 50-51 1.5498291444981689 1104 52-53 1.5118762415392624 1104 54-55 1.788004842212061 1104 56-57 1.6698180336940993 1104 58-59 1.827976291465557 1104 60-61 1.8484325127102572 1104 62-63 2.0078853903136356 1104 64-65 2.07728355301575 1104 66-67 2.0308289477904005 1104 68-69 2.068438398935424 1104 70-71 1.848780335445369 1104 72-73 2.0304495057728893 1104 74-75 2.135444421357814 1104 76-77 1.9909129612797969 1104 78-79 1.9718951958139215 1104 80-81 1.8612608102541692 1104 82-83 1.770117557048426 1104 84-85 2.0595549846321646 1104 86-87 1.9438750743455984 1104 88-89 1.8739793290150608 1104 90-91 1.9714516172833036 1104 92-93 1.7056610437105775 1104 94-95 1.648964857741614 1104 96-97 1.6850047723548727 1104 98-99 1.7151983467109346 1104 100-101 1.743890690875876 1104 102-103 1.6386520869646013 1104 104-105 1.5507625082217587 1104 106-107 1.6832551917898009 1104 108-109 1.5780679132141877 1104 110-111 1.604004535910736 1104 112-113 1.6150630323046684 1104 114-115 1.554799069294905 1104 116-117 1.5153663136619535 1104 118-119 1.4985272281754973 1104 120-121 1.5007083810366524 1104 122-123 1.6429093885164043 1104 124-125 1.4041488335820773 1105 1 0.4419758011633128 1105 2 0.45439641871794834 1105 3 0.38344890454618863 1105 4 0.5162538963064165 1105 5 0.695854980500556 1105 6 0.8638473839444032 1105 7 1.1446871912070975 1105 8 1.0006675663441769 1105 9 1.4841093470026365 1105 10-11 1.260316631621059 1105 12-13 1.270654391622699 1105 14-15 1.2736720029114537 1105 16-17 1.2764032919196069 1105 18-19 1.4393656679681541 1105 20-21 1.6759232958442425 1105 22-23 1.5219590689273161 1105 24-25 1.6879271922248833 1105 26-27 1.390072867455629 1105 28-29 1.388663256097466 1105 30-31 1.7658941257198748 1105 32-33 1.6435498840777214 1105 34-35 1.8510270448872568 1105 36-37 1.676575898155999 1105 38-39 1.7697981067490858 1105 40-41 1.8622155165942793 1105 42-43 1.9266061285934093 1105 44-45 1.7308370910499242 1105 46-47 1.560387028308071 1105 48-49 1.5714971529950326 1105 50-51 1.702087967178624 1105 52-53 1.6910798176223487 1105 54-55 1.84717187430293 1105 56-57 1.6255875168370864 1105 58-59 1.5652584680834138 1105 60-61 1.4912687264588307 1105 62-63 1.237748492719703 1105 64-65 1.6517556589946274 1105 66-67 1.863155512394549 1105 68-69 1.8955267908735998 1105 70-71 1.8025050152034723 1105 72-73 1.8936103659580716 1105 74-75 1.8691736017585967 1105 76-77 1.7099774985148066 1105 78-79 1.898608031111504 1105 80-81 1.8274770726785405 1105 82-83 1.720906796680353 1105 84-85 2.0527315351637014 1105 86-87 1.8924386654080294 1105 88-89 1.7420140774666315 1105 90-91 1.923075191406717 1105 92-93 1.6942938552940063 1105 94-95 1.5279107169086394 1105 96-97 1.6448478853988249 1105 98-99 1.71452991225188 1105 100-101 1.7081962435983051 1105 102-103 1.4698397838087658 1105 104-105 1.028622989713881 1105 106-107 1.2025599362359536 1105 108-109 1.3118278387702311 1105 110-111 1.4915189489919136 1105 112-113 1.4319086595535886 1105 114-115 1.2892275472204737 1105 116-117 1.3153954906079584 1105 118-119 1.4478674365539597 1105 120-121 1.444720981657028 1105 122-123 1.4308939813910015 1105 124-125 1.3764943181003773 1106 1 0.4034847210653467 1106 2 0.29380261054359735 1106 3 0.34701372979094103 1106 4 0.16157498247790159 1106 5 0.30818606491980916 1106 6 0.36637362456898614 1106 7 0.6967745181085618 1106 8 0.49078196189746137 1106 9 1.1177471466766917 1106 10-11 0.715968428051518 1106 12-13 0.6489864272522397 1106 14-15 0.8009422269992115 1106 16-17 0.7407098503959801 1106 18-19 0.7848196864363928 1106 20-21 0.7081732111970709 1106 22-23 0.6057090508121519 1106 24-25 0.5457897620999645 1106 26-27 0.5791124085559503 1106 28-29 0.5957902848300733 1106 30-31 0.8650159689071941 1106 32-33 0.6962755231474276 1106 34-35 0.7686768703889122 1106 36-37 0.6613384183626287 1106 38-39 0.8911123315588085 1106 40-41 0.9708557206433746 1106 42-43 1.096079326728404 1106 44-45 0.9733631077053282 1106 46-47 0.9609335131117618 1106 48-49 1.0725787923967438 1106 50-51 1.3295405944029994 1106 52-53 0.9821093249033233 1106 54-55 1.4794707993826037 1106 56-57 1.1261915748951736 1106 58-59 1.1129793840383222 1106 60-61 1.3779198997279742 1106 62-63 1.4307190223277715 1106 64-65 1.5414155947286439 1106 66-67 1.6648864648037325 1106 68-69 1.5387804438768313 1106 70-71 1.4024578499287088 1106 72-73 1.313710348435773 1106 74-75 1.6137904365997642 1106 76-77 1.157848342074967 1106 78-79 1.1643148580874083 1106 80-81 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-5.402636034429314 2216 88-89 -4.921667941754617 2216 90-91 -4.495844361295756 2216 92-93 -4.042336054617095 2216 94-95 -3.8958195683682497 2216 96-97 -4.168468170157745 2216 98-99 -4.040079532010974 2216 100-101 -4.33071752403551 2216 102-103 -4.347586878278516 2216 104-105 -4.154159794231504 2216 106-107 -4.376614950371721 2216 108-109 -4.424352179735354 2216 110-111 -4.027033976366869 2216 112-113 -4.15841166511818 2216 114-115 -4.064145241829706 2216 116-117 -3.895202495236873 2216 118-119 -4.103439347406912 2216 120-121 -3.8887784650005983 2216 122-123 -4.011585954763078 2216 124-125 -3.989894294716482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 112168.0 3 38696.0 4 19158.0 5 16326.0 6 17146.0 7 21104.0 8 23252.0 9 24531.0 10 26179.0 11 28237.0 12 31211.0 13 34162.0 14 36252.0 15 28963.0 16 20232.0 17 17515.0 18 15490.0 19 14422.0 20 14226.0 21 15264.0 22 18109.0 23 24209.0 24 33033.0 25 43994.0 26 53519.0 27 61327.0 28 69144.0 29 78352.0 30 87789.0 31 100203.0 32 117295.0 33 139663.0 34 169546.0 35 216292.0 36 300779.0 37 374978.0 38 129336.0 39 309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80956213316189 20.533477273063372 14.364487152445093 23.29247344132964 2 15.625826074246389 21.536163781173126 38.68247183184005 24.155538312740436 3 18.50124260858782 26.855117630891794 29.691211863112077 24.95242789740831 4 12.530631479736098 17.68552200091914 37.071490329565904 32.712356189778866 5 12.36046650399178 39.950614423589386 33.77283023591487 13.916088836503965 6 30.50372996528547 38.48379535144087 17.012101492374853 14.000373190898808 7 27.15908516405524 33.66793835240862 21.259007774005497 17.913968709530643 8 26.34921091536306 35.4434419694209 19.464191375328436 18.743155739887598 9 26.473802203458156 15.736870974350522 20.34091752834209 37.44840929384923 10-11 24.65003019553137 27.468564867717916 26.898863532330985 20.982541404419724 12-13 25.674517327237844 24.91553591108675 27.800707123128447 21.609239638546956 14-15 22.760126729902037 25.9993585756492 26.088652620121287 25.151862074327475 16-17 21.60939626015628 29.31600124402286 26.834156202620225 22.240446293200637 18-19 21.460633295242655 27.030592988062956 30.10413935317638 21.404634363518007 20-21 22.350068060982174 26.200300078664227 30.314340078656453 21.135291781697145 22-23 21.960091962101785 26.036342446092387 30.356648592500907 21.646916999304917 24-25 21.762948691184146 26.330304511121817 30.269833809368862 21.636912988325175 26-27 21.343314044462243 26.667878295452113 30.325681929949717 21.663125730135928 28-29 21.423810583626278 26.36862213038995 30.608759204959718 21.598808081024053 30-31 21.441374969823794 26.14003858666898 30.45279663939113 21.965789804116092 32-33 21.1480980294362 26.560394120535168 30.31902755819346 21.97248029183517 34-35 21.710158798405057 26.489738495191812 30.13700147839686 21.66310122800627 36-37 22.067957596524778 25.98490854882252 29.87006019903331 22.07707365561939 38-39 21.701210310379654 26.450281021832545 30.024825085387253 21.823683582400548 40-41 21.470006258819687 26.30267030015122 30.339239689469245 21.888083751559844 42-43 21.777772335375595 26.358136886910966 29.472396038941945 22.3916947387715 44-45 21.647292939156827 26.48955210419595 29.50918292136788 22.353972035279337 46-47 22.151313777996855 26.608360760602075 28.946071766567492 22.29425369483358 48-49 22.13534737165783 26.94733208405113 28.345495402075212 22.571825142215832 50-51 22.334303642646162 26.9383727193356 28.006529656658287 22.72079398135995 52-53 22.337116687120904 26.997456469791548 28.12332720030135 22.542099642786198 54-55 22.187434980718773 26.91033825304857 28.006895425542623 22.895331340690035 56-57 22.223587140012384 27.164619775909436 27.765110174457874 22.846682909620306 58-59 22.073766594840404 26.80543661180115 28.10340182809046 23.017394965267993 60-61 22.193265384108525 27.23365356469087 28.11032140664925 22.462759644551355 62-63 22.076912356595244 27.084559897574074 28.484752830581456 22.35377491524923 64-65 21.99514779881064 26.695955954429607 28.226534857740337 23.082361389019415 66-67 22.01618886595501 27.156927630729605 27.90121805350974 22.92566544980565 68-69 22.075436380984996 26.933875814202374 28.25094431051095 22.739743494301674 70-71 22.205123659732514 26.67655649076075 28.3616557835839 22.75666406592283 72-73 22.05906580448396 27.11822010944884 27.69318147558505 23.129532610482155 74-75 21.94787571951812 27.340765219534546 27.704700997494648 23.006658063452686 76-77 22.024160205538042 27.028610380969614 28.119394154146924 22.827835259345417 78-79 21.922654236111356 27.04567109487706 27.95300522358556 23.07866944542603 80-81 21.966623121241 27.545964202044814 27.49393127338396 22.993481403330225 82-83 22.309064711508803 27.064683908219124 27.609484415292357 23.01676696497972 84-85 21.780695231049783 27.045289419148027 27.603559462309875 23.57045588749232 86-87 21.899383768049237 27.177453965311877 27.804259666429274 23.11890260020961 88-89 21.965417689307333 27.15912026370863 27.55379298856761 23.321669058416422 90-91 21.8271440802907 27.201439121599364 27.78221915116316 23.189197646946774 92-93 22.07122120145299 27.160435274799095 27.580906501132034 23.187437022615878 94-95 21.73227991374327 26.68407247773509 27.934596809651673 23.64905079886997 96-97 21.990322475306645 26.662972252631057 27.898806228696536 23.447899043365762 98-99 21.61713272101542 26.831540527544007 27.67287187000833 23.878454881432244 100-101 21.528088638416474 26.81153490527365 27.935686883390442 23.724689572919434 102-103 21.92850299538539 26.41826702071064 27.915775061436456 23.737454922467517 104-105 21.600022857969208 26.806352960476314 27.804173289840996 23.789450891713482 106-107 21.53278024638758 26.57692075195619 27.94501499663448 23.94528400502175 108-109 21.773075919827743 26.47321909290545 27.535860327140572 24.21784466012624 110-111 21.410293664592412 26.824929565008652 27.62293687916864 24.141839891230298 112-113 21.401440053739616 26.787335892806887 27.985806273042645 23.825417780410856 114-115 21.30146691520192 26.96554391707371 27.539714780526392 24.19327438719797 116-117 21.153968009000117 26.841433969921603 27.82376144403052 24.180836577047756 118-119 21.08867245652504 26.654003902624485 27.679866613356584 24.57745702749389 120-121 20.978731998140994 26.880443611911886 27.709300311759126 24.431524078187998 122-123 21.060332848501858 26.932560090951092 27.583476958907372 24.423630101639674 124-125 21.104174243774654 26.808917696424395 27.395427162277404 24.691480897523547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 651.0 1 508.5 2 621.0 3 953.5 4 732.0 5 537.0 6 1107.0 7 2425.5 8 3894.0 9 4583.5 10 3783.5 11 2577.5 12 2233.5 13 2306.5 14 2460.5 15 2623.0 16 2903.0 17 3257.5 18 3567.0 19 3943.5 20 4459.5 21 5055.0 22 5803.0 23 6723.0 24 7826.0 25 9603.5 26 12426.5 27 15621.0 28 18581.0 29 23133.0 30 30318.5 31 37531.5 32 46191.0 33 54156.0 34 59710.5 35 66022.0 36 75424.5 37 83884.0 38 86221.5 39 86878.5 40 85696.0 41 83346.5 42 80525.5 43 81970.5 44 86056.5 45 88018.0 46 86626.5 47 83751.0 48 85352.5 49 85085.5 50 81901.5 51 78606.0 52 76686.0 53 77402.5 54 77776.5 55 74265.5 56 68839.5 57 63844.0 58 60376.5 59 55258.5 60 46877.0 61 39408.0 62 32929.5 63 25698.0 64 19611.0 65 15539.0 66 11922.0 67 9516.0 68 7322.0 69 5178.0 70 3696.0 71 2779.0 72 2192.0 73 1919.0 74 1651.0 75 1262.0 76 871.5 77 594.0 78 408.0 79 229.5 80 160.0 81 120.0 82 71.5 83 38.0 84 26.5 85 22.0 86 22.0 87 18.0 88 12.0 89 7.0 90 5.0 91 4.5 92 1.0 93 1.0 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10950816179840625 2 1.166221105414337E-4 3 0.0 4 0.18624551053466962 5 0.0 6 3.8874036847144565E-5 7 0.0396903916209346 8 0.0 9 0.0 10-11 4.0817738689501794E-4 12-13 6.21984589554313E-4 14-15 4.2761440531859026E-4 16-17 0.0043150180900330464 18-19 0.0015743984923093548 20-21 0.00332373015043086 22-23 0.0018270797318157945 24-25 0.004820380569045926 26-27 0.00450938827426877 28-29 0.0034403522609722944 30-31 7.774807369428914E-4 32-33 0.0 34-35 0.0011662211054143368 36-37 0.0010690360132964757 38-39 5.053624790128794E-4 40-41 0.0015938355107329273 42-43 6.8029564482503E-4 44-45 0.0010495989948729034 46-47 5.831105527071685E-5 48-49 1.9437018423572283E-5 50-51 0.04143972327905611 52-53 7.774807369428913E-5 54-55 0.13456247854639092 56-57 5.247994974364517E-4 58-59 0.0 60-61 0.0 62-63 6.025475711307407E-4 64-65 0.06293706565552705 66-67 0.02981638626175988 68-69 0.0012245321606850538 70-71 0.00272118257930012 72-73 0.07010932545382521 74-75 0.004528825292692342 76-77 0.0015743984923093548 78-79 0.0015549614738857827 80-81 5.831105527071685E-5 82-83 5.831105527071685E-5 84-85 0.0 86-87 1.166221105414337E-4 88-89 0.001030161976449331 90-91 3.3042931320072884E-4 92-93 0.0022935681739815294 94-95 0.0034986633162430113 96-97 0.0022935681739815294 98-99 0.0 100-101 0.0010690360132964757 102-103 0.0014577763817679211 104-105 1.5549614738857826E-4 106-107 1.9437018423572283E-5 108-109 0.0 110-111 1.36059128965006E-4 112-113 2.72118257930012E-4 114-115 2.332442210828674E-4 116-117 0.0 118-119 0.0015355244554622102 120-121 0.002818367671417981 122-123 0.0015355244554622102 124-125 0.009504702009126847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2572411.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.74618208958144 #Duplication Level Percentage of deduplicated Percentage of total 1 75.29458359985215 34.44439731718049 2 11.352593279299752 10.386755986876098 3 4.809810012433024 6.600913339351592 4 2.50325437911984 4.580573225750333 5 1.4832820941835068 3.3927246385367185 6 0.9604246502733658 2.636145656083677 7 0.6748038827513642 2.1608790906570325 8 0.46833483170011253 1.713962439187746 9 0.36407551667927535 1.4989558392331717 >10 1.82806317062928 15.001217280538617 >50 0.15104525664257668 4.826822389886379 >100 0.10121445213542453 8.722680427438563 >500 0.006495883486880906 1.9823909211274768 >1k 0.001755644185643229 1.0453271091006657 >5k 1.755644185643229E-4 0.596733158205059 >10k+ 8.778220928216145E-5 0.40952118084624445 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10198 0.3964374277671803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9059 0.3521598997982826 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5801 0.2255082877502856 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.8874036847144565E-5 0.0 12-13 0.0 0.0 0.0 7.774807369428913E-5 0.0 14-15 0.0 0.0 0.0 7.774807369428913E-5 0.0 16-17 0.0 0.0 0.0 7.774807369428913E-5 0.0 18-19 0.0 0.0 0.0 9.718509211786142E-5 0.0 20-21 0.0 0.0 0.0 1.3605912896500597E-4 0.0 22-23 0.0 0.0 0.0 3.109922947771565E-4 0.0 24-25 0.0 0.0 0.0 8.746658290607527E-4 0.0 26-27 0.0 0.0 0.0 0.0014577763817679211 0.0 28-29 0.0 0.0 0.0 0.0028766787266886976 0.0 30-31 0.0 0.0 0.0 0.006628023282438149 0.0 32-33 0.0 0.0 0.0 0.013683660970194887 0.0 34-35 0.0 0.0 0.0 0.023071740868780298 0.0 36-37 0.0 0.0 0.0 0.03827148927601383 0.0 38-39 0.0 0.0 0.0 0.06559993717955645 0.0 40-41 0.0 0.0 0.0 0.09858455744435862 0.0 42-43 1.9437018423572283E-5 3.8874036847144565E-5 0.0 0.13011140132739285 0.0 44-45 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.16566170802410657 0.0 46-47 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.21149419746689002 0.0 48-49 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.26204988238660154 0.0 50-51 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.3206913669705191 0.0 52-53 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.380226954401921 0.0 54-55 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.44279471670740017 0.0 56-57 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.5164610165327391 0.0 58-59 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.6022754528728107 0.0 60-61 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.6993050488432836 0.0 62-63 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.7957126602242022 0.0 64-65 3.8874036847144565E-5 3.8874036847144565E-5 0.0 0.8944527138159493 0.0 66-67 3.8874036847144565E-5 3.8874036847144565E-5 0.0 1.0100252253625102 0.0 68-69 3.8874036847144565E-5 5.831105527071685E-5 0.0 1.1464730946959873 0.0 70-71 3.8874036847144565E-5 7.774807369428913E-5 0.0 1.3040295660374646 0.0 72-73 3.8874036847144565E-5 7.774807369428913E-5 0.0 1.4709352432406795 0.0 74-75 3.8874036847144565E-5 7.774807369428913E-5 0.0 1.6334092802433204 0.0 76-77 3.8874036847144565E-5 7.774807369428913E-5 0.0 1.807973142705423 0.0 78-79 3.8874036847144565E-5 7.774807369428913E-5 0.0 2.0150162629533153 0.0 80-81 3.8874036847144565E-5 7.774807369428913E-5 0.0 2.253372419881582 0.0 82-83 3.8874036847144565E-5 7.774807369428913E-5 0.0 2.5131092970757782 0.0 84-85 5.831105527071685E-5 7.774807369428913E-5 0.0 2.7707275392618054 0.0 86-87 7.774807369428913E-5 7.774807369428913E-5 0.0 3.032816295685254 0.0 88-89 7.774807369428913E-5 7.774807369428913E-5 0.0 3.3252656748863227 0.0 90-91 7.774807369428913E-5 7.774807369428913E-5 0.0 3.6736353560920083 0.0 92-93 7.774807369428913E-5 7.774807369428913E-5 0.0 4.052579467277974 0.0 94-95 7.774807369428913E-5 7.774807369428913E-5 0.0 4.430843282819114 3.8874036847144565E-5 96-97 7.774807369428913E-5 7.774807369428913E-5 0.0 4.807202270554744 3.8874036847144565E-5 98-99 7.774807369428913E-5 7.774807369428913E-5 0.0 5.2130277782205106 3.8874036847144565E-5 100-101 7.774807369428913E-5 7.774807369428913E-5 0.0 5.682548395260322 3.8874036847144565E-5 102-103 7.774807369428913E-5 7.774807369428913E-5 0.0 6.205229257688604 3.8874036847144565E-5 104-105 7.774807369428913E-5 7.774807369428913E-5 0.0 6.7216125261476485 3.8874036847144565E-5 106-107 7.774807369428913E-5 7.774807369428913E-5 0.0 7.231290023250562 3.8874036847144565E-5 108-109 7.774807369428913E-5 7.774807369428913E-5 0.0 7.7552731659132235 3.8874036847144565E-5 110-111 7.774807369428913E-5 7.774807369428913E-5 0.0 8.347208124984693 3.8874036847144565E-5 112-113 7.774807369428913E-5 7.774807369428913E-5 0.0 8.985344876848995 3.8874036847144565E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7155 0.0 64.04194 1 GTATCAA 12755 0.0 49.162632 1 ATCAACG 16740 0.0 35.95737 3 TATCAAC 17540 0.0 35.540546 2 TCAACGC 16820 0.0 35.50289 4 CAACGCA 16845 0.0 35.285645 5 AACGCAG 17140 0.0 34.122925 6 GTACATG 14115 0.0 32.92831 1 ACGCATA 215 1.2187229E-10 30.441511 7 TACATGG 15175 0.0 30.083065 2 ACATGGG 14920 0.0 28.480179 3 CGCAGAG 20585 0.0 28.457922 8 ACGCAGA 20365 0.0 28.398397 7 ATATAGG 1240 0.0 27.395243 2 GAGTACT 12895 0.0 26.682415 12-13 GCAGAGT 21250 0.0 26.615791 9 CATGGGA 9300 0.0 26.145636 4 TAAGGTG 1210 0.0 25.569456 5 GTACTTT 13880 0.0 25.17454 14-15 CTTACTA 1555 0.0 24.47752 9 >>END_MODULE