FastQCFastQC Report
Thu 26 May 2016
SRR1364504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4633240
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79750.049.115961
GGTATCA43500.048.991551
ATCAACG90250.041.008913
CAACGCA92050.040.9046555
TCAACGC91850.040.80094
TATCAAC101000.038.3525922
AACGCAG96700.037.8078466
GTACATG135950.031.176561
ACGCAGA113450.031.12437
CGCAGAG113050.030.6573548
TACATGG132500.030.1778622
ACATGGG136900.027.1216583
GCAGAGT138700.023.3134049
AGAGTAC112050.023.05277310-11
CAGAGTA125450.021.23086510-11
GAGTACA95700.020.7137991
CATGGGG88450.019.8711224
GAGTACT69700.019.58572412-13
AGTACAT100300.018.2719482
CATGGGA65000.018.1488914
Produced by FastQC (version 0.11.5)