##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364504_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4633240 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.879430808678162 30.0 24.0 31.0 2.0 34.0 2 25.181582218922397 30.0 25.0 31.0 2.0 34.0 3 25.13912855798534 30.0 25.0 31.0 2.0 34.0 4 27.890665279588365 35.0 25.0 35.0 2.0 37.0 5 28.36903009557027 35.0 27.0 35.0 2.0 37.0 6 28.481882656629054 35.0 27.0 37.0 2.0 37.0 7 28.291543714549647 35.0 27.0 37.0 2.0 37.0 8 27.8733380960192 35.0 26.0 37.0 2.0 37.0 9 28.483812839395327 35.0 25.0 39.0 2.0 39.0 10-11 28.52108675570443 35.0 25.5 39.0 2.0 39.0 12-13 28.34973150538284 35.0 24.0 39.0 2.0 39.0 14-15 28.913594050815412 35.0 24.5 39.5 2.0 41.0 16-17 29.126239953035025 36.0 25.0 40.0 2.0 41.0 18-19 29.06030423634433 36.0 25.0 40.0 2.0 41.0 20-21 28.829798585870797 35.0 23.0 40.0 2.0 41.0 22-23 28.854135011352746 35.0 24.0 40.0 2.0 41.0 24-25 28.85289354749592 35.0 24.0 40.0 2.0 41.0 26-27 28.92011324688555 35.0 24.5 40.0 2.0 41.0 28-29 28.831866685084304 35.0 24.0 40.0 2.0 41.0 30-31 28.49236516994587 35.0 23.0 40.0 2.0 41.0 32-33 28.484781815748807 35.0 23.5 40.0 2.0 41.0 34-35 28.197800027626457 35.0 21.5 39.5 2.0 41.0 36-37 27.972820639552452 34.0 20.5 39.0 2.0 41.0 38-39 27.815887262477233 34.0 19.0 39.0 2.0 41.0 40-41 27.845509405944867 34.0 20.0 39.0 2.0 41.0 42-43 27.71150922464625 34.0 19.0 39.0 2.0 41.0 44-45 27.369422045911715 33.5 17.0 39.0 2.0 41.0 46-47 27.145317099912802 33.0 15.5 39.0 2.0 40.0 48-49 26.910055921989795 33.0 14.5 39.0 2.0 40.0 50-51 26.893975705985444 33.0 15.0 39.0 2.0 40.0 52-53 26.69692849927912 33.0 13.0 39.0 2.0 40.0 54-55 26.57225030432268 33.0 11.0 38.5 2.0 40.0 56-57 26.152874338475883 33.0 8.5 38.0 2.0 40.0 58-59 25.92318636634407 32.5 8.0 38.0 2.0 40.0 60-61 25.517945865096564 32.0 7.5 38.0 2.0 40.0 62-63 25.460388302785958 32.0 7.0 37.5 2.0 40.0 64-65 25.278925654617503 32.0 6.5 37.0 2.0 40.0 66-67 25.03157876561542 32.0 4.0 37.0 2.0 40.0 68-69 24.853128480285932 31.5 2.0 36.0 2.0 39.5 70-71 24.73261885850938 32.0 2.0 36.0 2.0 39.0 72-73 24.712628851516435 32.0 2.0 36.0 2.0 39.0 74-75 24.452987110531723 31.5 2.0 35.0 2.0 39.0 76-77 24.17866061330732 31.0 2.0 35.0 2.0 37.5 78-79 23.885799677979122 31.0 2.0 35.0 2.0 37.0 80-81 23.899816974730427 31.0 2.0 35.0 2.0 37.0 82-83 23.715025446555757 31.0 2.0 35.0 2.0 36.0 84-85 23.547728047759236 31.0 2.0 35.0 2.0 36.0 86-87 23.350434685015237 31.0 2.0 35.0 2.0 36.0 88-89 23.131339516191694 31.0 2.0 35.0 2.0 35.0 90-91 22.96774611287134 31.0 2.0 35.0 2.0 35.0 92-93 22.867124517616183 31.0 2.0 34.5 2.0 35.0 94-95 22.808034981999636 31.0 2.0 34.0 2.0 35.0 96-97 22.67852787682054 31.0 2.0 34.0 2.0 35.0 98-99 22.592866439036182 30.5 2.0 34.0 2.0 35.0 100-101 22.529661316918613 31.0 2.0 34.0 2.0 35.0 102-103 21.477700809800485 29.0 2.0 33.5 2.0 34.5 104-105 22.19672216418748 30.0 2.0 34.0 2.0 35.0 106-107 22.283141279104903 30.0 2.0 34.0 2.0 35.0 108-109 22.226071820151773 30.0 2.0 34.0 2.0 35.0 110-111 22.115759813866752 30.0 2.0 34.0 2.0 35.0 112-113 22.089577595807686 30.0 2.0 34.0 2.0 35.0 114-115 22.038446098194783 30.0 2.0 34.0 2.0 35.0 116-117 21.845090044979322 30.0 2.0 34.0 2.0 35.0 118-119 21.69644093550086 30.0 2.0 34.0 2.0 35.0 120-121 21.542859532422234 30.0 2.0 34.0 2.0 35.0 122-123 21.269087398882853 30.0 2.0 34.0 2.0 35.0 124-125 20.334828867056316 28.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6461100749563755 1101 2 1.3684791748310907 1101 3 1.6078133999141073 1101 4 2.0116238242562687 1101 5 1.5051382593122788 1101 6 1.527185269113449 1101 7 1.2907201965778334 1101 8 1.8082974043728086 1101 9 2.1824303492938597 1101 10-11 2.2056503433814427 1101 12-13 2.044855934492663 1101 14-15 2.3343384526873088 1101 16-17 2.048013659668811 1101 18-19 1.918520403407058 1101 20-21 2.3047118707750407 1101 22-23 2.1391163763346803 1101 24-25 1.9968198631125098 1101 26-27 1.6592483698608902 1101 28-29 1.5636728660241275 1101 30-31 1.6643656047960036 1101 32-33 1.9005270226895874 1101 34-35 2.230442199187756 1101 36-37 2.2454353422542077 1101 38-39 2.257454037569328 1101 40-41 2.0673649354923995 1101 42-43 2.0669329680013355 1101 44-45 2.204104599580482 1101 46-47 2.1171050354737986 1101 48-49 2.211932375160746 1101 50-51 2.0786579331488753 1101 52-53 1.9888518581883723 1101 54-55 2.1008683555157432 1101 56-57 2.2919240145507693 1101 58-59 2.2707055824277127 1101 60-61 2.4041481661805797 1101 62-63 2.115740125049154 1101 64-65 2.09658029026388 1101 66-67 2.2229794713301345 1101 68-69 2.0283437221342453 1101 70-71 2.070934510079173 1101 72-73 1.921876896305342 1101 74-75 1.8817713771313436 1101 76-77 2.153528806771458 1101 78-79 2.092931699625506 1101 80-81 1.9270970467412063 1101 82-83 1.9693633298206095 1101 84-85 1.7820820119341896 1101 86-87 1.7421174271115447 1101 88-89 1.807404163040509 1101 90-91 1.8498885855707385 1101 92-93 1.8523213999145156 1101 94-95 1.778688162639888 1101 96-97 1.8337533850740684 1101 98-99 1.7509232717072187 1101 100-101 1.7230398048494386 1101 102-103 1.7059763076730228 1101 104-105 1.6684565695976623 1101 106-107 1.7867581983547254 1101 108-109 1.8401155623651562 1101 110-111 1.9404818083308903 1101 112-113 1.8353215862315935 1101 114-115 1.7716993938455907 1101 116-117 1.8264266555724546 1101 118-119 1.857771310609369 1101 120-121 1.8396694260131774 1101 122-123 1.8762045475420592 1101 124-125 1.883040947774365 1102 1 0.7647859787815889 1102 2 0.5991281176645344 1102 3 0.6968233254864238 1102 4 1.182784930006779 1102 5 1.1206577577859207 1102 6 1.0678080296526709 1102 7 0.987670015728149 1102 8 1.1679428148536282 1102 9 1.4742042279411827 1102 10-11 1.2185655076696946 1102 12-13 1.5285170402431767 1102 14-15 1.7198776725226566 1102 16-17 1.2305988367660774 1102 18-19 1.4104967857362674 1102 20-21 1.5467390377266632 1102 22-23 1.5151659251979517 1102 24-25 1.2809764010683757 1102 26-27 1.2368282286796415 1102 28-29 1.0670422846530556 1102 30-31 0.9038912764226339 1102 32-33 0.8116362999115587 1102 34-35 1.2137044340497063 1102 36-37 1.4473930894483544 1102 38-39 1.4783410474271825 1102 40-41 1.1385332357310851 1102 42-43 1.093639683985515 1102 44-45 1.4405902472819108 1102 46-47 1.3080485150104195 1102 48-49 1.4708912762943704 1102 50-51 1.2885401974659096 1102 52-53 1.478011809999412 1102 54-55 1.2431100874970724 1102 56-57 1.471395459486132 1102 58-59 1.490085629149565 1102 60-61 1.8078397144908642 1102 62-63 1.6538233017965673 1102 64-65 1.731370436918585 1102 66-67 1.6669643854759109 1102 68-69 1.534232622308604 1102 70-71 1.7275239559060829 1102 72-73 1.5982458945504519 1102 74-75 1.7273755448560237 1102 76-77 1.5855542950091746 1102 78-79 1.4486448285789493 1102 80-81 1.1913647042722815 1102 82-83 1.157000518835332 1102 84-85 1.2283603455214482 1102 86-87 1.3679081154688895 1102 88-89 1.1142716546751537 1102 90-91 1.020621901411591 1102 92-93 1.0164068676126767 1102 94-95 1.2578893910634754 1102 96-97 1.3409464625657606 1102 98-99 1.4083062272593878 1102 100-101 1.2425485059480827 1102 102-103 1.1171599984736709 1102 104-105 1.1843133626051845 1102 106-107 1.3984110121049405 1102 108-109 1.5353933683566154 1102 110-111 1.3453403449437822 1102 112-113 1.2731682618137867 1102 114-115 1.2887503308218733 1102 116-117 1.497648773404066 1102 118-119 1.5619642865318148 1102 120-121 1.5302898589238687 1102 122-123 1.670996231842274 1102 124-125 1.5596765652871625 1103 1 1.3081800166738553 1103 2 1.0804290775813499 1103 3 1.2310893124761897 1103 4 1.8788000001231175 1103 5 1.282556189485014 1103 6 1.35115414919634 1103 7 1.2225019774498271 1103 8 1.5721473294073647 1103 9 2.2592615157992846 1103 10-11 1.704026653088711 1103 12-13 1.8495064851032623 1103 14-15 2.0791541561809623 1103 16-17 1.8435025710439703 1103 18-19 1.6224064714138784 1103 20-21 1.9529214522140386 1103 22-23 1.794400379913629 1103 24-25 1.8721176883403494 1103 26-27 1.553118741241125 1103 28-29 1.3999596258539597 1103 30-31 1.621285383353392 1103 32-33 1.6812747213816834 1103 34-35 1.484069203848609 1103 36-37 1.8553602017655244 1103 38-39 1.8571659911749059 1103 40-41 1.8617059700357466 1103 42-43 1.9289456336944966 1103 44-45 1.8288384167051888 1103 46-47 2.047872998736352 1103 48-49 2.1176007996610835 1103 50-51 1.9987173164192313 1103 52-53 2.0326091923134406 1103 54-55 1.9356787807956906 1103 56-57 2.0617677248926327 1103 58-59 2.300883971239511 1103 60-61 2.224726265229222 1103 62-63 2.0408043859544875 1103 64-65 1.8900696742546046 1103 66-67 1.877229123432567 1103 68-69 1.8597043735896897 1103 70-71 2.009055701635912 1103 72-73 1.9314692418182915 1103 74-75 1.9907462928246744 1103 76-77 2.0019629479273213 1103 78-79 2.2146695077260823 1103 80-81 1.7122562448028091 1103 82-83 1.819749955608053 1103 84-85 1.7827603869445419 1103 86-87 1.778266037776632 1103 88-89 1.8603567143230002 1103 90-91 1.8403924294433303 1103 92-93 1.7964850184800945 1103 94-95 1.6236506496210765 1103 96-97 1.7394914059189759 1103 98-99 1.6616727342921571 1103 100-101 1.4128271009161644 1103 102-103 1.5014290323152117 1103 104-105 1.714683606170162 1103 106-107 1.5163518263928104 1103 108-109 1.7949265800976661 1103 110-111 1.9035281690900625 1103 112-113 1.7701635023847473 1103 114-115 1.7382419820957722 1103 116-117 1.8682534695713002 1103 118-119 1.9363646026999284 1103 120-121 1.921130053837846 1103 122-123 1.9755837515147192 1103 124-125 1.8805823538124038 1104 1 1.2727145878449875 1104 2 1.0163597896700196 1104 3 1.1309192470034866 1104 4 1.37807853384016 1104 5 1.1137430187662432 1104 6 0.9323228889705746 1104 7 0.8745062966789092 1104 8 1.2887975384241521 1104 9 1.846590849970056 1104 10-11 1.555859998197377 1104 12-13 1.0478778082556595 1104 14-15 1.3693395555473913 1104 16-17 1.0883532737346258 1104 18-19 0.4868790691023186 1104 20-21 -3.8970163146896333 1104 22-23 -0.8146193486825659 1104 24-25 0.6045141131639404 1104 26-27 0.815857286763169 1104 28-29 0.4349277091394974 1104 30-31 1.1476233346569238 1104 32-33 1.0104971192139836 1104 34-35 1.282468509641756 1104 36-37 1.4644215056304866 1104 38-39 1.3731517098290134 1104 40-41 1.389172378349766 1104 42-43 1.402979912707572 1104 44-45 1.5041422156500062 1104 46-47 1.4595384683987405 1104 48-49 1.5453803391027954 1104 50-51 1.5184758052750347 1104 52-53 1.5706502850717214 1104 54-55 1.5374067042259867 1104 56-57 1.717842581887247 1104 58-59 1.6713620998109384 1104 60-61 1.7610762069737795 1104 62-63 1.4302449074515131 1104 64-65 1.5592085580902975 1104 66-67 1.5286401327936794 1104 68-69 1.4670529785094821 1104 70-71 1.6504977700593138 1104 72-73 1.5187426570671434 1104 74-75 1.488132094814759 1104 76-77 1.5482678671049577 1104 78-79 1.5443163460307403 1104 80-81 1.304891571653588 1104 82-83 1.2807050203825767 1104 84-85 1.2075267207679907 1104 86-87 1.115665773601222 1104 88-89 1.3011043997283913 1104 90-91 1.327825126927248 1104 92-93 1.0045315946491584 1104 94-95 1.139024542392118 1104 96-97 1.2353088819589573 1104 98-99 1.2077020550557371 1104 100-101 0.6937207336878579 1104 102-103 1.084391961899275 1104 104-105 1.2321619826726078 1104 106-107 1.3691955228532926 1104 108-109 1.4275622296111052 1104 110-111 1.5032714019362352 1104 112-113 1.4616486573991168 1104 114-115 1.4165570951881072 1104 116-117 1.5029440907877785 1104 118-119 1.4420945629846038 1104 120-121 1.4564482539217032 1104 122-123 1.2624592346974133 1104 124-125 1.3855835230009355 1105 1 1.1011124959070742 1105 2 0.6868178763594486 1105 3 0.8489111431670224 1105 4 1.3355922887419744 1105 5 1.0026309964601907 1105 6 0.9660673161851534 1105 7 0.8616035278059897 1105 8 1.1106857248645774 1105 9 1.450854310390234 1105 10-11 1.218747949798015 1105 12-13 1.361612860516832 1105 14-15 1.5744199229411926 1105 16-17 1.33144488361728 1105 18-19 0.9369214707305389 1105 20-21 1.1392267333071437 1105 22-23 0.9879951077565146 1105 24-25 1.0835183179144146 1105 26-27 0.9666264802562452 1105 28-29 0.6406709397894446 1105 30-31 1.0188009443606525 1105 32-33 0.9314570361627048 1105 34-35 0.8140336795464798 1105 36-37 1.0522658441278097 1105 38-39 0.9608562627387904 1105 40-41 1.1064840778443 1105 42-43 1.3799411045008512 1105 44-45 1.5727864445554296 1105 46-47 1.7324817564539678 1105 48-49 1.4075028248260217 1105 50-51 1.4649508787090646 1105 52-53 1.4635263455783765 1105 54-55 1.469166269287797 1105 56-57 1.8316981293248844 1105 58-59 1.544014074701721 1105 60-61 1.888469615803757 1105 62-63 1.686406097008227 1105 64-65 1.4305709643407596 1105 66-67 1.6642256293866389 1105 68-69 1.1627706936301827 1105 70-71 1.2754748452229556 1105 72-73 1.2143258984526213 1105 74-75 1.5228680677970026 1105 76-77 1.4203970438983156 1105 78-79 1.1756445327693754 1105 80-81 1.2011694542187854 1105 82-83 1.3139098752292249 1105 84-85 1.283165913664643 1105 86-87 1.277633563274236 1105 88-89 1.3920240211517445 1105 90-91 1.4587108456861024 1105 92-93 1.3885627686989892 1105 94-95 1.2489181003267191 1105 96-97 1.4067084711757083 1105 98-99 1.1304454497207779 1105 100-101 1.2372131554452075 1105 102-103 1.2169168996480941 1105 104-105 1.2819286339317983 1105 106-107 1.3969501376414897 1105 108-109 1.4789149813369917 1105 110-111 1.3883742654905866 1105 112-113 1.443446244140734 1105 114-115 1.3524933604154192 1105 116-117 1.4488120288198978 1105 118-119 1.5391986715625912 1105 120-121 1.525340555649823 1105 122-123 1.5771139829342502 1105 124-125 1.206411016403056 1106 1 -0.5959773478725765 1106 2 -0.35085411631150976 1106 3 -0.31513758783674106 1106 4 -0.7189534107581395 1106 5 -1.2259682879410008 1106 6 -0.586207317248828 1106 7 -0.5113927879379432 1106 8 -0.15933476093502108 1106 9 -0.17664290402678873 1106 10-11 -2.5504047073597214 1106 12-13 -0.7489897077603125 1106 14-15 -0.4961298236238889 1106 16-17 -0.6188889688819295 1106 18-19 -3.374208346923833 1106 20-21 -5.6917581144876905 1106 22-23 -6.53274899394231 1106 24-25 -6.339540882175452 1106 26-27 -5.259770770441669 1106 28-29 -2.0938781354273672 1106 30-31 -3.7330873791889836 1106 32-33 -4.416704222905619 1106 34-35 -3.746563936161479 1106 36-37 -6.117269659910907 1106 38-39 -6.462241333993802 1106 40-41 -2.8743603048416695 1106 42-43 -1.4294050861575016 1106 44-45 -0.6947800906499353 1106 46-47 -0.5442603145017344 1106 48-49 -0.33613341788553797 1106 50-51 -0.46256385945372713 1106 52-53 -0.6944361427897263 1106 54-55 -0.6537746099393473 1106 56-57 -0.5440002778074202 1106 58-59 -0.3781294883245643 1106 60-61 -0.08824990293555146 1106 62-63 -0.37827329908952834 1106 64-65 -0.51872670062723 1106 66-67 -0.8225760014317771 1106 68-69 -0.743724951904003 1106 70-71 -0.4906924848223291 1106 72-73 -0.4516233762331865 1106 74-75 -0.5361499534827274 1106 76-77 -0.5822463946495979 1106 78-79 -0.5657632150981726 1106 80-81 -0.9395846588804382 1106 82-83 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88-89 -4.440255535646791 2216 90-91 -4.4635451627197895 2216 92-93 -4.244058277421601 2216 94-95 -7.188020461698262 2216 96-97 -9.036473514787716 2216 98-99 -6.111036221165083 2216 100-101 -5.144315834414403 2216 102-103 -6.191706449348329 2216 104-105 -8.715988815172713 2216 106-107 -5.486855531317779 2216 108-109 -7.674739445373788 2216 110-111 -5.267529622234001 2216 112-113 -4.733977025584579 2216 114-115 -5.138267004317687 2216 116-117 -4.691890101035639 2216 118-119 -4.918687101352205 2216 120-121 -5.7152415207658045 2216 122-123 -8.363230280159815 2216 124-125 -7.10362097156723 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 600151.0 3 84570.0 4 47275.0 5 38253.0 6 37644.0 7 40963.0 8 44774.0 9 47352.0 10 48390.0 11 48568.0 12 48308.0 13 47517.0 14 46183.0 15 40179.0 16 34915.0 17 30917.0 18 28421.0 19 27473.0 20 27424.0 21 30447.0 22 38141.0 23 51185.0 24 67919.0 25 85946.0 26 102412.0 27 116496.0 28 131204.0 29 146713.0 30 163196.0 31 181714.0 32 210905.0 33 251734.0 34 303308.0 35 371716.0 36 461627.0 37 435968.0 38 112964.0 39 368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22072805372352 18.405293019230275 14.22101388609526 24.152965040950942 2 15.041081250303614 19.725520465058537 40.426757058731454 24.806641225906397 3 19.030121703350268 24.49253203126029 28.638560504787225 27.838785760602214 4 11.472861688493278 16.202893011273396 38.09923307236415 34.22501222786918 5 13.994449202558757 37.49714405165887 34.01498179751337 14.493424948269004 6 32.227339008995244 36.34072619809217 17.471977822269075 13.959956970643514 7 28.32049869991736 32.328340420278394 20.86564114783689 18.485519731967358 8 25.488752946417453 35.84179640328251 19.766103934477677 18.903346715822366 9 25.658538859620496 16.6312251456081 20.085910074371075 37.62432592040033 10-11 24.53607721969531 26.707106357416126 27.45979472685121 21.297021696037348 12-13 25.405069215883557 23.781015105856255 27.833385441348053 22.980530236912138 14-15 22.48513365945854 25.28157493306936 25.39417468686735 26.83911672060475 16-17 22.673164594589547 28.59316392106509 25.66491920890667 23.06875227543869 18-19 22.65095435569994 27.52616155038669 27.464131317151075 22.358752776762287 20-21 23.305110981993245 26.657430548929472 27.709920059423514 22.327538409653773 22-23 22.99344904757819 26.54223149001113 27.334476336647395 23.129843125763283 24-25 22.813530093058542 26.558051418619026 27.51404361439846 23.114374873923975 26-27 22.822893280147827 26.946396007827815 27.09376379043535 23.136946921589008 28-29 22.765586912539497 27.076358304704755 27.05686788528194 23.10118689747381 30-31 22.84413844695627 26.823447571939933 27.284990665644898 23.047423315458897 32-33 22.816803367148715 26.788268940211523 27.12558817181092 23.269339520828836 34-35 22.98529996833202 26.710486635657805 27.3200359465068 22.98417744950338 36-37 23.062702791603883 26.47862369022497 27.354508591683874 23.104164926487268 38-39 22.890518880152545 26.68979626284379 27.26822683336783 23.151458023635833 40-41 23.139974942673213 26.710676394534367 27.11208803356119 23.03726062923123 42-43 22.98779638845627 26.690389615435674 26.95807686537604 23.363737130732012 44-45 22.92241418054281 26.798431663356443 27.157121904337167 23.12203225176358 46-47 23.126502912636866 26.898048752376113 26.804439459047497 23.17100887593952 48-49 23.164978550036423 26.808428891347162 26.795305290991017 23.231287267625394 50-51 23.150592470052718 27.01248357367713 26.664703134686352 23.1722208215838 52-53 23.223040935117098 26.84924880405703 26.617701466905373 23.310008793920495 54-55 23.226329018840516 26.87925427950704 26.555316561025812 23.339100140626638 56-57 22.937230811004543 26.975725410685726 26.806882820436417 23.280160957873314 58-59 23.1304268238915 26.83215899317047 26.68926951735434 23.34814466558369 60-61 23.080139971622238 26.710859027470125 26.920820778808046 23.28818022209959 62-63 23.121710926055155 26.907136765687024 26.75227873021807 23.21887357803975 64-65 23.023205065278006 26.825142231068988 26.72631759348355 23.425335110169453 66-67 23.03831394509922 26.991661740869127 26.53586942925754 23.43415488477411 68-69 23.046320336088712 26.869397392823807 26.745210685172395 23.33907158591509 70-71 22.943158820343253 26.527070951622278 26.996778718150193 23.532991509884273 72-73 22.995808731022578 26.808743325082023 26.66435077418365 23.53109716971175 74-75 22.90825814405434 26.987753162180063 26.663840683623143 23.440148010142458 76-77 23.11657978328854 26.733650130387122 26.74026580777259 23.409504278551747 78-79 23.0254579758826 26.826798119309032 26.78252244006269 23.365221464745677 80-81 23.063721289827967 26.871214479073902 26.564617231520742 23.50044699957739 82-83 23.031042904623096 26.910016510702278 26.676337030713317 23.38260355396131 84-85 23.0831897933726 26.870864109304556 26.530868385016532 23.51507771230631 86-87 23.105391263357397 26.76321742623255 26.678630560442485 23.45276074996757 88-89 23.110000005396046 26.731016307388966 26.641485121214064 23.51749856600092 90-91 23.051822612805235 26.81458357885067 26.706329318288464 23.42726449005563 92-93 23.07583227312711 26.7709508854633 26.618235863608252 23.534980977801336 94-95 23.076967074607104 26.709774490617644 26.67947087820689 23.53378755656836 96-97 23.121948640026936 26.588692863217055 26.70184408980441 23.587514406951595 98-99 22.90748889170196 26.645626919394005 26.66991932353371 23.77696486537033 100-101 22.94862504229041 26.640039670698666 26.770415544093453 23.64091974291747 102-103 22.73413179197139 26.37124711472166 27.096153570137044 23.798467523169908 104-105 22.73551050159005 26.53930095305746 26.923228426972244 23.801960118380247 106-107 22.684706454692357 26.525095058429343 26.910033944120297 23.880164542758 108-109 22.673097244740973 26.518591134893217 26.896776638746296 23.91153498161952 110-111 22.658290331464762 26.59688953390493 26.893147011567986 23.851673123062326 112-113 22.769760973666138 26.550535004400995 26.892469878347853 23.78723414358502 114-115 22.761564859270305 26.743795406097338 26.74021250692973 23.75442722770263 116-117 22.481230872303883 26.633156392813568 27.08342551608491 23.80218721879764 118-119 22.630396543803197 26.654154443398703 26.86747954029181 23.847969472506296 120-121 22.60444676119663 26.727028133969434 26.907065680464115 23.761459424369825 122-123 22.46697171498649 26.673273564756073 27.00843172722684 23.851322993030596 124-125 22.635642291528796 26.744227604511345 26.68817753931036 23.9319525646495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 416.0 1 501.5 2 637.5 3 707.0 4 688.5 5 633.0 6 645.0 7 791.0 8 1048.5 9 1232.0 10 1249.0 11 1331.0 12 1497.5 13 1632.5 14 1836.5 15 2094.5 16 2372.0 17 2736.0 18 3162.0 19 3735.0 20 4561.5 21 5670.5 22 7268.5 23 9884.0 24 13524.0 25 17928.5 26 23732.0 27 30258.0 28 36512.0 29 43141.5 30 50571.5 31 59270.0 32 68019.0 33 74763.5 34 80996.0 35 88395.5 36 97108.0 37 105873.0 38 114147.5 39 120981.5 40 128269.5 41 136096.5 42 141307.5 43 146382.5 44 152701.5 45 158317.5 46 162900.0 47 168444.0 48 173045.5 49 174818.0 50 174560.5 51 174356.5 52 175073.0 53 174251.0 54 170608.0 55 161270.0 56 148427.0 57 136245.5 58 125625.0 59 113286.0 60 97472.0 61 81346.5 62 66065.0 63 51830.0 64 39257.0 65 29664.5 66 22242.0 67 16833.0 68 12989.5 69 10105.0 70 7706.5 71 5550.5 72 4082.5 73 3096.5 74 2286.5 75 1628.0 76 1143.5 77 771.0 78 531.0 79 359.0 80 236.5 81 173.5 82 121.0 83 84.5 84 64.0 85 54.0 86 39.0 87 26.0 88 21.5 89 19.5 90 16.5 91 11.5 92 11.5 93 13.0 94 12.5 95 10.0 96 6.0 97 7.5 98 5.5 99 4.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10042648341117663 2 0.0337776588305376 3 0.04448291044711692 4 0.0523391838108969 5 0.055123412557950814 6 0.0026763129041448316 7 0.0015755713064723605 8 0.0015324049693087344 9 0.0055252911569441685 10-11 0.06411280227227599 12-13 0.005082836201016999 14-15 0.00990667437905224 16-17 0.01924139479068643 18-19 0.01773057298995951 20-21 0.13199186746207836 22-23 0.01527009177163281 24-25 0.013349189767851438 26-27 0.005902996607125899 28-29 0.004197926289162659 30-31 0.0018669440823268382 32-33 0.005179960459635158 34-35 0.01728811803403234 36-37 0.0020935673524358767 38-39 0.007748357520870924 40-41 0.031533009298029026 42-43 0.012841985306178829 44-45 0.11119648453350141 46-47 0.0033022247930174138 48-49 0.007877856532361803 50-51 0.009323928827343285 52-53 0.0106620852794157 54-55 0.00874118327563433 56-57 0.00488858768378068 58-59 0.013780853139487704 60-61 0.0311660954321382 62-63 0.0061943693829803765 64-65 0.0061727862143985636 66-67 0.006874239193307491 68-69 0.006399409484507602 70-71 0.003161934197235628 72-73 7.985772375270868E-4 74-75 9.49659417599779E-4 76-77 0.006593658001743921 78-79 0.019424851723631842 80-81 0.002719479241308458 82-83 0.003982094603344527 84-85 0.0020180262623995305 86-87 0.0028705614213811502 88-89 0.004694339166544362 90-91 0.003582805984580984 92-93 0.0021691084424722224 94-95 0.0027842287470538976 96-97 0.0012949901149087895 98-99 0.002255441116799475 100-101 0.002104358936726783 102-103 4.640381245089829E-4 104-105 0.0043921748063989775 106-107 7.985772375270868E-4 108-109 0.0013813227892360421 110-111 0.002330982206835821 112-113 0.004187134704871753 114-115 0.002600771814108486 116-117 0.005028878279562466 118-119 0.0036799302431991436 120-121 0.006852656024725678 122-123 0.0043921748063989775 124-125 0.05522053681656896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4633240.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.066476247755475 #Duplication Level Percentage of deduplicated Percentage of total 1 80.40004370659469 49.90147403033861 2 10.034759501168885 12.456443244624744 3 3.846973817790087 7.16304327262817 4 1.8695871215385036 4.641547386883162 5 1.0714396593377669 3.3250242083595367 6 0.6682016367530286 2.4883752609745904 7 0.44682919804925586 1.9413179665269436 8 0.3095959114553589 1.5372421827796992 9 0.2268127390001246 1.2669720730055636 >10 1.0610448185783168 11.28558388184186 >50 0.04782448586302909 2.0026856059885123 >100 0.01638737650857494 1.6956142310104863 >500 3.928787019717357E-4 0.15431014984119282 >1k 1.0714866049174195E-4 0.14036650519692762 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 2.1583168581813158E-5 0.0 0.0 9 0.0 0.0 2.1583168581813158E-5 0.0 0.0 10-11 0.0 0.0 2.1583168581813158E-5 2.1583168581813158E-5 0.0 12-13 0.0 0.0 2.1583168581813158E-5 6.474950574543948E-5 1.0791584290906579E-5 14-15 0.0 0.0 2.1583168581813158E-5 7.554109003634606E-5 4.3166337163626316E-5 16-17 0.0 0.0 2.1583168581813158E-5 1.8345693294541187E-4 1.079158429090658E-4 18-19 0.0 0.0 2.1583168581813158E-5 2.4820643869085133E-4 1.7266534865450527E-4 20-21 2.1583168581813158E-5 0.0 2.1583168581813158E-5 3.561222815999171E-4 2.0504010152722502E-4 22-23 2.1583168581813158E-5 0.0 2.1583168581813158E-5 6.259118888725816E-4 2.158316858181316E-4 24-25 2.1583168581813158E-5 0.0 2.1583168581813158E-5 9.172846647270593E-4 2.589980229817579E-4 26-27 2.1583168581813158E-5 0.0 2.1583168581813158E-5 0.0011007415976724711 2.589980229817579E-4 28-29 2.1583168581813158E-5 0.0 2.1583168581813158E-5 0.0015539881378905476 2.589980229817579E-4 30-31 2.1583168581813158E-5 0.0 2.1583168581813158E-5 0.0030971846914901882 2.589980229817579E-4 32-33 4.3166337163626316E-5 0.0 2.1583168581813158E-5 0.006798698103271145 3.021643601453842E-4 34-35 5.39579214545329E-5 0.0 2.1583168581813158E-5 0.012496654608869819 3.237475287271974E-4 36-37 6.474950574543948E-5 0.0 2.1583168581813158E-5 0.022165914133522112 3.237475287271974E-4 38-39 6.474950574543948E-5 0.0 2.1583168581813158E-5 0.03888207820013641 3.669138658908237E-4 40-41 6.474950574543948E-5 0.0 2.1583168581813158E-5 0.058166639327986464 3.8849703447263684E-4 42-43 6.474950574543948E-5 0.0 2.1583168581813158E-5 0.07786128065889097 3.8849703447263684E-4 44-45 6.474950574543948E-5 0.0 2.1583168581813158E-5 0.10154880817743092 3.992886187635434E-4 46-47 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.13179761894484204 4.424549559271698E-4 48-49 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.1651436144037434 5.179960459635158E-4 50-51 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.20214795693726204 5.611623831271421E-4 52-53 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.2405875801814713 6.474950574543948E-4 54-55 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.28147689305971635 7.44619316072554E-4 56-57 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.330718892179123 7.769940689452737E-4 58-59 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.38772004040369157 7.877856532361803E-4 60-61 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.45022489661662246 8.201604061089E-4 62-63 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.5186974989424247 8.633267432725264E-4 64-65 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.5901485785325171 8.74118327563433E-4 66-67 6.474950574543948E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.672995571133807 8.849099118543396E-4 68-69 8.633267432725263E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.7688032564684756 8.849099118543396E-4 70-71 9.712425861815922E-5 2.1583168581813158E-5 2.1583168581813158E-5 0.8779601315709957 9.49659417599779E-4 72-73 1.079158429090658E-4 2.1583168581813158E-5 2.1583168581813158E-5 0.9955560255890046 0.0011439079348360974 74-75 1.079158429090658E-4 2.1583168581813158E-5 2.1583168581813158E-5 1.1141231621931953 0.00123024060916335 76-77 1.079158429090658E-4 2.1583168581813158E-5 2.1583168581813158E-5 1.248856092065164 0.0012518237777451632 78-79 1.079158429090658E-4 2.1583168581813158E-5 2.1583168581813158E-5 1.4034887033695642 0.0012626153620360699 80-81 1.079158429090658E-4 3.237475287271974E-5 2.1583168581813158E-5 1.5791972787941053 0.0013165732834906027 82-83 1.079158429090658E-4 4.3166337163626316E-5 3.237475287271974E-5 1.770413360844679 0.0014029059578178553 84-85 1.079158429090658E-4 4.3166337163626316E-5 6.474950574543948E-5 1.9628381003358342 0.0014029059578178553 86-87 1.079158429090658E-4 4.3166337163626316E-5 6.474950574543948E-5 2.1681695746389136 0.0014136975421087617 88-89 1.079158429090658E-4 4.3166337163626316E-5 6.474950574543948E-5 2.3964979150659147 0.0014244891263996684 90-91 1.079158429090658E-4 4.3166337163626316E-5 6.474950574543948E-5 2.657708212827309 0.0014244891263996684 92-93 1.079158429090658E-4 4.3166337163626316E-5 6.474950574543948E-5 2.937091538534589 0.0014244891263996684 94-95 1.079158429090658E-4 5.39579214545329E-5 6.474950574543948E-5 3.2195180910119054 0.0015324049693087342 96-97 1.079158429090658E-4 6.474950574543948E-5 6.474950574543948E-5 3.5058404054182386 0.0015971544750541737 98-99 1.079158429090658E-4 6.474950574543948E-5 6.474950574543948E-5 3.8193359290690747 0.0016403208122178 100-101 1.079158429090658E-4 6.474950574543948E-5 6.474950574543948E-5 4.179407930519464 0.0016403208122178 102-103 1.079158429090658E-4 6.474950574543948E-5 6.474950574543948E-5 4.564041577815956 0.0017266534865450528 104-105 1.079158429090658E-4 6.474950574543948E-5 6.474950574543948E-5 4.949797549878703 0.001802194576581399 106-107 1.079158429090658E-4 6.474950574543948E-5 6.474950574543948E-5 5.344316288385666 0.0018453609137450253 108-109 1.079158429090658E-4 6.474950574543948E-5 6.474950574543948E-5 5.761421812813495 0.001877735666617745 110-111 1.079158429090658E-4 6.474950574543948E-5 6.474950574543948E-5 6.217873453565971 0.0019424851723631844 112-113 1.2949901149087896E-4 6.474950574543948E-5 6.474950574543948E-5 6.7084804585991655 0.0019640683409449973 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7975 0.0 49.11596 1 GGTATCA 4350 0.0 48.99155 1 ATCAACG 9025 0.0 41.00891 3 CAACGCA 9205 0.0 40.904655 5 TCAACGC 9185 0.0 40.8009 4 TATCAAC 10100 0.0 38.352592 2 AACGCAG 9670 0.0 37.807846 6 GTACATG 13595 0.0 31.17656 1 ACGCAGA 11345 0.0 31.1243 7 CGCAGAG 11305 0.0 30.657354 8 TACATGG 13250 0.0 30.177862 2 ACATGGG 13690 0.0 27.121658 3 GCAGAGT 13870 0.0 23.313404 9 AGAGTAC 11205 0.0 23.052773 10-11 CAGAGTA 12545 0.0 21.230865 10-11 GAGTACA 9570 0.0 20.713799 1 CATGGGG 8845 0.0 19.871122 4 GAGTACT 6970 0.0 19.585724 12-13 AGTACAT 10030 0.0 18.271948 2 CATGGGA 6500 0.0 18.148891 4 >>END_MODULE