FastQCFastQC Report
Thu 26 May 2016
SRR1364504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4633240
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65690.14177983441393063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59590.12861410157902461No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63750.062.3991851
GTATCAA114150.048.328031
TCAACGC137900.037.720254
ATCAACG139650.037.418233
TATCAAC149250.036.8079072
CAACGCA141350.036.6121675
AACGCAG147900.034.347236
ACGCAGA175850.028.786837
CGCAGAG178850.028.4299478
GTACATG141050.025.4117551
TACATGG135400.025.1710832
GCAGAGT204250.023.87549
AGAGTAC173800.023.59504710-11
GAGTACT121000.023.51976212-13
ACATGGG138650.023.20593
CAGAGTA188650.022.76233310-11
GTACTTT133300.021.57284414-15
GTGGTAT39300.021.5490971
TGGTATC35400.021.03932
AGTACTT132750.020.67633412-13
Produced by FastQC (version 0.11.5)