##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364504_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4633240 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.081014581588693 31.0 28.0 31.0 25.0 34.0 2 29.27980290250451 31.0 28.0 33.0 25.0 34.0 3 29.400682675622242 31.0 28.0 34.0 26.0 34.0 4 32.84562228591655 35.0 32.0 37.0 27.0 37.0 5 31.98078558417004 35.0 32.0 37.0 22.0 37.0 6 32.03957446624824 35.0 32.0 37.0 23.0 37.0 7 31.49813996253162 35.0 32.0 37.0 18.0 37.0 8 31.63979396707272 35.0 32.0 37.0 18.0 37.0 9 32.250076620248464 37.0 31.0 39.0 16.0 39.0 10-11 32.4465680603638 37.0 31.5 39.0 17.0 39.0 12-13 32.18981889563243 37.0 31.0 39.0 14.5 39.0 14-15 33.17652539043952 38.0 32.0 40.0 16.5 41.0 16-17 33.22295801642048 38.0 32.0 40.0 17.0 41.0 18-19 33.06173951705502 38.0 31.5 40.0 15.5 41.0 20-21 32.92368331880067 38.0 31.0 40.0 13.0 41.0 22-23 32.89007972822474 38.0 31.0 40.0 13.5 41.0 24-25 32.686817648125285 38.0 31.0 40.0 10.5 41.0 26-27 32.77951034265438 38.0 31.0 40.0 10.5 41.0 28-29 32.674677007882174 37.5 31.0 40.0 10.0 41.0 30-31 32.16727182274175 37.0 30.0 40.0 9.0 41.0 32-33 32.129101557441444 37.0 30.0 40.0 9.0 41.0 34-35 31.943470767756473 37.0 30.0 40.0 8.0 41.0 36-37 31.948183992195524 37.0 30.0 40.0 8.0 41.0 38-39 31.72313143718003 37.0 30.0 40.0 6.5 41.0 40-41 31.442856079115263 37.0 30.0 40.0 2.0 41.0 42-43 31.339115716000038 37.0 29.5 40.0 2.0 41.0 44-45 31.286012595937184 37.0 30.0 40.0 2.0 41.0 46-47 31.175995955314207 36.5 29.5 40.0 2.0 41.0 48-49 30.925441159965814 36.0 29.0 40.0 2.0 41.0 50-51 30.580916701919175 36.0 28.0 40.0 2.0 41.0 52-53 30.48632393314398 36.0 28.0 40.0 2.0 41.0 54-55 30.009911314760295 35.0 27.0 40.0 2.0 41.0 56-57 30.017572368364256 35.0 28.0 39.0 2.0 41.0 58-59 29.76691041258385 35.0 27.5 39.0 2.0 41.0 60-61 29.47359007951239 35.0 26.5 39.0 2.0 41.0 62-63 29.19723336153534 34.5 26.0 39.0 2.0 40.5 64-65 28.701788920927903 34.0 26.0 38.0 2.0 40.0 66-67 28.29428456112785 34.0 25.0 38.0 2.0 40.0 68-69 28.097400307344323 34.0 24.5 37.5 2.0 40.0 70-71 28.181423690549163 34.0 26.0 37.0 2.0 40.0 72-73 27.955319279812834 34.0 26.0 36.5 2.0 39.0 74-75 27.587863676390604 34.0 25.0 36.0 2.0 39.0 76-77 27.511563398399392 34.0 25.5 36.0 2.0 39.0 78-79 27.110961443827648 33.0 25.0 35.0 2.0 37.5 80-81 26.895462678384888 33.0 24.0 35.0 2.0 37.0 82-83 26.76924625100362 33.0 24.5 35.0 2.0 36.5 84-85 26.340133686146196 33.0 22.5 35.0 2.0 36.0 86-87 26.221190031166095 33.0 23.0 35.0 2.0 36.0 88-89 26.187414962315785 33.0 23.0 35.0 2.0 35.5 90-91 26.029889882673896 33.0 23.0 35.0 2.0 35.0 92-93 26.056676753200783 33.0 23.5 35.0 2.0 35.0 94-95 26.07794621042726 33.0 24.0 35.0 2.0 35.0 96-97 25.961059755160534 33.0 23.0 35.0 2.0 35.0 98-99 25.82705946594608 33.0 23.0 35.0 2.0 35.0 100-101 25.733345779627214 33.0 21.5 35.0 2.0 35.0 102-103 24.589662633491898 30.5 19.5 34.0 2.0 35.0 104-105 25.56322821610795 32.5 21.5 35.0 2.0 35.0 106-107 25.665605278379708 33.0 22.0 35.0 2.0 35.0 108-109 25.743635878996123 33.0 23.0 35.0 2.0 35.0 110-111 25.67093761600953 33.0 22.5 35.0 2.0 35.0 112-113 25.62186418143675 33.0 21.0 35.0 2.0 35.0 114-115 25.576182325974912 33.0 20.0 35.0 2.0 35.0 116-117 25.495185118836925 33.0 19.5 35.0 2.0 35.0 118-119 25.374079801607515 33.0 19.0 35.0 2.0 35.0 120-121 25.21221510217472 33.0 18.0 35.0 2.0 35.0 122-123 24.922069653201646 33.0 4.5 35.0 2.0 35.0 124-125 24.137021285320856 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7828629046903437 1101 2 0.7582302557377645 1101 3 0.6792768930253672 1101 4 0.6883799705391596 1101 5 0.9098953816390605 1101 6 0.9582881598785349 1101 7 1.3995845848290145 1101 8 1.0878527145299195 1101 9 1.8214300416365319 1101 10-11 1.5492254535034888 1101 12-13 1.68694725267882 1101 14-15 1.5942654948435617 1101 16-17 1.555677985653496 1101 18-19 1.6569773260018934 1101 20-21 1.8953103646428815 1101 22-23 1.9093126068432653 1101 24-25 2.048711445665731 1101 26-27 1.6955700066800645 1101 28-29 1.777328821255793 1101 30-31 2.181929263973913 1101 32-33 2.0434011078363667 1101 34-35 2.107865621370408 1101 36-37 1.9983556727036955 1101 38-39 2.1592429617255746 1101 40-41 2.3148105372634937 1101 42-43 2.251183437844581 1101 44-45 2.0966665222094 1101 46-47 1.9429364207511313 1101 48-49 1.995407677269803 1101 50-51 2.2210878763267417 1101 52-53 2.097778103658012 1101 54-55 2.4014437630785714 1101 56-57 2.1142260565802466 1101 58-59 2.288555040990488 1101 60-61 2.346297651169884 1101 62-63 2.2796793367315615 1101 64-65 2.4746496997504224 1101 66-67 2.6480762806227283 1101 68-69 2.604112485295193 1101 70-71 2.1893006346941277 1101 72-73 2.4241892187615015 1101 74-75 2.5647643670568776 1101 76-77 2.3553419756095444 1101 78-79 2.3856032473476816 1101 80-81 2.4185442356550553 1101 82-83 2.273727373002007 1101 84-85 2.525059224303895 1101 86-87 2.3286248744013385 1101 88-89 2.2532690395823387 1101 90-91 2.176372553939011 1101 92-93 2.1233612004703097 1101 94-95 1.9955768229714046 1101 96-97 1.927392242167393 1101 98-99 2.0219810243824483 1101 100-101 2.0756757829030903 1101 102-103 1.8316118562937227 1101 104-105 1.919600048113555 1101 106-107 2.059843272488642 1101 108-109 2.0580474186559066 1101 110-111 1.9943583826789677 1101 112-113 1.8705847309504087 1101 114-115 1.941397457881795 1101 116-117 1.9946606857497855 1101 118-119 1.897239961181711 1101 120-121 1.8273864044129873 1101 122-123 1.882195317554359 1101 124-125 1.7182127192433896 1102 1 0.4816811747403946 1102 2 -1.671220028938258 1102 3 0.0055716187601930756 1102 4 0.49233298912069046 1102 5 1.0105279323249334 1102 6 0.5649859462332714 1102 7 1.374038509069642 1102 8 1.1007068667370739 1102 9 1.78876494929316 1102 10-11 1.544777919661179 1102 12-13 1.621199152870112 1102 14-15 1.7223023447298615 1102 16-17 1.629163686960922 1102 18-19 1.722119184380503 1102 20-21 1.883669400637892 1102 22-23 1.8355391260088183 1102 24-25 2.063998322355026 1102 26-27 1.739290276515689 1102 28-29 1.7320056617751547 1102 30-31 2.0454455912300205 1102 32-33 1.7654938998056267 1102 34-35 1.9794429494221433 1102 36-37 1.3930509367555146 1102 38-39 1.4523150222834218 1102 40-41 1.6030333717225567 1102 42-43 1.4238536227570364 1102 44-45 1.4763623364279646 1102 46-47 1.2683561697622423 1102 48-49 1.284325626248183 1102 50-51 1.4667789421831046 1102 52-53 1.2199405128804983 1102 54-55 1.496072090176682 1102 56-57 1.151769999385472 1102 58-59 1.1819591765515725 1102 60-61 1.2034256961312053 1102 62-63 1.5961250197565207 1102 64-65 1.6920995781494632 1102 66-67 1.7814254136163683 1102 68-69 1.7416450015603786 1102 70-71 1.1803346801407244 1102 72-73 1.4740720614589549 1102 74-75 1.672895408310918 1102 76-77 1.5941827601755314 1102 78-79 1.3743526901275196 1102 80-81 1.4730890361561286 1102 82-83 1.141583669448778 1102 84-85 1.266431481228942 1102 86-87 1.3805010135286295 1102 88-89 1.5346458248028867 1102 90-91 1.2722369928535748 1102 92-93 1.2869793791080362 1102 94-95 1.3053817112734514 1102 96-97 1.312340738096161 1102 98-99 1.131206756386355 1102 100-101 1.0229539387441662 1102 102-103 0.7999233099188992 1102 104-105 1.1231656223465691 1102 106-107 1.400214155407518 1102 108-109 1.310558788768592 1102 110-111 1.1110762571732344 1102 112-113 1.1135069768721202 1102 114-115 1.3786149684196083 1102 116-117 1.0017222169625803 1102 118-119 0.5623287778435824 1102 120-121 0.9373615622685207 1102 122-123 1.2914368492185737 1102 124-125 1.2824401017475218 1103 1 0.679596740665815 1103 2 0.5645499404452856 1103 3 0.53980396709742 1103 4 0.5621449930826543 1103 5 0.9391696070578348 1103 6 0.9419214896436898 1103 7 1.1228789442093898 1103 8 0.9840807777957714 1103 9 1.7753392523214089 1103 10-11 1.495778995393593 1103 12-13 1.5032034776384506 1103 14-15 1.2582644418937932 1103 16-17 1.31102806788995 1103 18-19 1.4767902018810588 1103 20-21 1.581536886185603 1103 22-23 1.4117374545728651 1103 24-25 1.8067358957788713 1103 26-27 1.3075833456147734 1103 28-29 1.4450491109381716 1103 30-31 2.02889220404667 1103 32-33 1.7421616196615162 1103 34-35 1.8225821260888182 1103 36-37 1.7554906865825686 1103 38-39 1.805757489268224 1103 40-41 2.0342540391697774 1103 42-43 1.9471922431707327 1103 44-45 1.8404925606339866 1103 46-47 1.6884919325459862 1103 48-49 1.7144910791054642 1103 50-51 1.791720928716373 1103 52-53 1.7291400679344697 1103 54-55 2.0042451379724504 1103 56-57 1.8662012631139184 1103 58-59 1.9810366497146248 1103 60-61 1.8586730089136125 1103 62-63 2.0041070140953607 1103 64-65 2.0852533550480565 1103 66-67 2.3329357010560123 1103 68-69 2.400177102345303 1103 70-71 2.1264565661877626 1103 72-73 2.149031360450568 1103 74-75 2.2177445380736955 1103 76-77 1.9986856196371185 1103 78-79 2.033842788035372 1103 80-81 2.0559926401062576 1103 82-83 1.9383056444004865 1103 84-85 2.096262343970558 1103 86-87 2.045077416595067 1103 88-89 1.9755799613779281 1103 90-91 2.053329498795815 1103 92-93 1.7866084733371217 1103 94-95 1.699536105996092 1103 96-97 1.6166813394918655 1103 98-99 1.8018758943492088 1103 100-101 1.8046315987338275 1103 102-103 1.6111978767023665 1103 104-105 1.6190871558746203 1103 106-107 1.6894134885520522 1103 108-109 1.6502051813665375 1103 110-111 1.7039420097139235 1103 112-113 1.5649302088879438 1103 114-115 1.478202815672649 1103 116-117 1.6008619304387537 1103 118-119 1.6385312451947165 1103 120-121 1.5621989167417212 1103 122-123 1.7116653384450444 1103 124-125 1.5650379814204776 1104 1 0.7151091224743915 1104 2 0.6236177161072121 1104 3 0.6015827766539488 1104 4 0.560685074367683 1104 5 0.9643408732435859 1104 6 1.0058713738281924 1104 7 1.341004444776928 1104 8 1.2173785069738763 1104 9 1.7475227457529101 1104 10-11 1.249765186544515 1104 12-13 1.4084066447764059 1104 14-15 1.3811836061826668 1104 16-17 1.185326503773453 1104 18-19 1.2390199974038012 1104 20-21 1.5723932291678437 1104 22-23 1.573620489730665 1104 24-25 1.6645764221714714 1104 26-27 1.5434864737550527 1104 28-29 1.5013297302798634 1104 30-31 2.0058645212596673 1104 32-33 1.9821317158798948 1104 34-35 1.8267334222376945 1104 36-37 1.7313396556104763 1104 38-39 1.9080429237010392 1104 40-41 2.06642251395267 1104 42-43 2.035160199060144 1104 44-45 1.7734441135778347 1104 46-47 1.8312258847611318 1104 48-49 1.5608239531081054 1104 50-51 1.9309245527743215 1104 52-53 1.7923865846149205 1104 54-55 2.1218305401946402 1104 56-57 1.9159230314758808 1104 58-59 2.0042576148505518 1104 60-61 2.128879145846078 1104 62-63 2.186516567951191 1104 64-65 2.2637464981884463 1104 66-67 2.2601781277091355 1104 68-69 2.2726696658055374 1104 70-71 2.066777446782364 1104 72-73 2.2537691516095784 1104 74-75 2.375424196733693 1104 76-77 2.187053380519611 1104 78-79 2.2664182720896235 1104 80-81 2.1278012607252563 1104 82-83 2.0887898052800864 1104 84-85 2.4049413296749336 1104 86-87 2.235877423419197 1104 88-89 2.011873576236283 1104 90-91 2.163889904490329 1104 92-93 1.9103999208852507 1104 94-95 1.8921989333480198 1104 96-97 1.9406932546449376 1104 98-99 1.948265169413375 1104 100-101 1.9942830748505784 1104 102-103 1.9103065118411884 1104 104-105 1.7894722685905116 1104 106-107 1.933943178986901 1104 108-109 1.848274128813138 1104 110-111 1.8959557102670281 1104 112-113 1.8556759935506726 1104 114-115 1.7493485777860833 1104 116-117 1.8555776080794715 1104 118-119 1.8048271186916693 1104 120-121 1.7276372029653828 1104 122-123 1.843983239416911 1104 124-125 1.6316254659294245 1105 1 0.39668705946277427 1105 2 0.47478166409951683 1105 3 0.3540884799059185 1105 4 0.5442181457738826 1105 5 0.7056739804349021 1105 6 0.8281010098638006 1105 7 1.0848615205369825 1105 8 1.003868882270222 1105 9 1.5329363992635763 1105 10-11 1.2279555596042897 1105 12-13 1.3517395245831665 1105 14-15 1.335581913099638 1105 16-17 1.2506703017665046 1105 18-19 1.507382045803162 1105 20-21 1.6688551627566426 1105 22-23 1.5522415887808663 1105 24-25 1.7712768113345518 1105 26-27 1.4264255496045735 1105 28-29 1.5732482035975863 1105 30-31 1.8601743904949046 1105 32-33 1.6628568675938524 1105 34-35 1.8513751051816172 1105 36-37 1.591628061234701 1105 38-39 1.729048969893654 1105 40-41 1.8679285638729048 1105 42-43 1.8508323999935499 1105 44-45 1.7484659201294868 1105 46-47 1.5918233097242265 1105 48-49 1.744828754277087 1105 50-51 1.9416614837537907 1105 52-53 1.8631640347767053 1105 54-55 2.099146228237931 1105 56-57 1.9081794690681377 1105 58-59 1.8651826040404664 1105 60-61 1.6895147788697749 1105 62-63 1.5276212654924564 1105 64-65 1.9143851319427512 1105 66-67 2.163121173469346 1105 68-69 2.09541995655956 1105 70-71 1.9339166508618675 1105 72-73 2.1514975426215486 1105 74-75 2.141825332910944 1105 76-77 2.0307895883416407 1105 78-79 2.084561975401236 1105 80-81 2.053520131406813 1105 82-83 1.99540567075789 1105 84-85 2.2387678576245946 1105 86-87 2.163256574119245 1105 88-89 2.0828604252082066 1105 90-91 2.104128490531153 1105 92-93 1.9133574111561735 1105 94-95 1.7792861931031894 1105 96-97 1.814994606278379 1105 98-99 1.8906357652354657 1105 100-101 1.9293074720951253 1105 102-103 1.7797121994371707 1105 104-105 1.356342711580357 1105 106-107 1.614371239732126 1105 108-109 1.5716171104583552 1105 110-111 1.7168224368240566 1105 112-113 1.720428684889189 1105 114-115 1.5781490683403057 1105 116-117 1.5755278071341507 1105 118-119 1.6794699199202903 1105 120-121 1.6785013574675958 1105 122-123 1.7374914103504508 1105 124-125 1.5902110035261536 1106 1 0.3676474518125161 1106 2 0.3222885483604436 1106 3 0.30837106705216044 1106 4 0.20208861312943327 1106 5 0.4096610642862615 1106 6 0.616683117768325 1106 7 0.7915848134755734 1106 8 0.5208260996625889 1106 9 1.279627077351897 1106 10-11 0.9219545542896697 1106 12-13 0.8588137004073388 1106 14-15 0.8496853595564815 1106 16-17 0.8267964325082602 1106 18-19 0.9060389507851809 1106 20-21 0.7703968159508037 1106 22-23 0.661530682578352 1106 24-25 0.5039219842304448 1106 26-27 0.6077661284897005 1106 28-29 0.5763427149365086 1106 30-31 0.8974636311616848 1106 32-33 0.7702922394122496 1106 34-35 0.8309463739805096 1106 36-37 0.8065009680626432 1106 38-39 0.9503938410054147 1106 40-41 1.1937364303744111 1106 42-43 1.1977682397443417 1106 44-45 1.0487903039236208 1106 46-47 1.0479793532248678 1106 48-49 0.9679975940020782 1106 50-51 1.3303844172990438 1106 52-53 1.074223453842233 1106 54-55 1.4961140130087642 1106 56-57 1.262052207394877 1106 58-59 1.1633111116359238 1106 60-61 1.4967023419628482 1106 62-63 1.5647482573360847 1106 64-65 1.6191171789743173 1106 66-67 1.616986501917662 1106 68-69 1.5705668543157643 1106 70-71 1.4505517804787615 1106 72-73 1.362743859504004 1106 74-75 1.6928812358421084 1106 76-77 1.2956447247441254 1106 78-79 1.2406071363806106 1106 80-81 1.3878848704804234 1106 82-83 1.1944097015040498 1106 84-85 1.4446225059807958 1106 86-87 1.5444459004028488 1106 88-89 1.2346464241243353 1106 90-91 1.3480831397981738 1106 92-93 1.3627337289949857 1106 94-95 1.2332472332717508 1106 96-97 1.1473511923984354 1106 98-99 1.056134564854137 1106 100-101 0.7621102258753965 1106 102-103 0.6664043708863865 1106 104-105 0.8816166778406505 1106 106-107 0.8774285176179397 1106 108-109 0.8267621562193384 1106 110-111 0.869339357153347 1106 112-113 0.699493197700594 1106 114-115 0.7741316998129868 1106 116-117 1.042470527350801 1106 118-119 0.7768664529700544 1106 120-121 0.7842942410241562 1106 122-123 0.9885479774192447 1106 124-125 0.9781671237631535 1107 1 0.36668000899112485 1107 2 0.4428737213785219 1107 3 0.22883715490348777 1107 4 0.22714688738625455 1107 5 0.33659212202782385 1107 6 0.6222719108654502 1107 7 0.7694191642207358 1107 8 0.6466460995219983 1107 9 1.381175604748357 1107 10-11 0.8165581074988708 1107 12-13 1.1215349393453948 1107 14-15 0.9042842008561323 1107 16-17 0.9912092981683358 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104-105 -4.319215124791462 2216 106-107 -4.390205036131324 2216 108-109 -4.386810551431019 2216 110-111 -4.105673839727466 2216 112-113 -4.237239737544105 2216 114-115 -4.085588664706716 2216 116-117 -3.9448967591683157 2216 118-119 -4.134644834683307 2216 120-121 -4.028288166275033 2216 122-123 -4.101852467139135 2216 124-125 -4.020512115971098 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 211174.0 3 72345.0 4 35658.0 5 28932.0 6 28917.0 7 33890.0 8 37531.0 9 39904.0 10 42877.0 11 45411.0 12 48866.0 13 51333.0 14 50878.0 15 42404.0 16 32972.0 17 28698.0 18 25261.0 19 23920.0 20 23363.0 21 25366.0 22 31224.0 23 42997.0 24 59746.0 25 78753.0 26 96532.0 27 111740.0 28 126395.0 29 143544.0 30 161036.0 31 184284.0 32 214798.0 33 254777.0 34 309811.0 35 393712.0 36 559108.0 37 698298.0 38 235765.0 39 1020.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.89216640123484 19.79825452373838 14.249033177790496 25.060545897236292 2 15.81981519983614 20.45661107035473 38.273611381801125 25.449962348008004 3 19.16328530358885 24.238632145107957 29.414642884892643 27.183439666410546 4 13.32543416591711 17.099499238912337 36.123577284992734 33.45148931017782 5 13.72726213189906 37.89311583254914 34.10775181082785 14.271870224723951 6 32.46122148753053 36.97651058569 16.757078637910954 13.805189288868513 7 27.945535089482604 31.96411757072663 20.930510234463913 19.15983710532685 8 25.773799759995164 36.41866167088258 19.16997608584921 18.637562483273044 9 25.374791722423183 16.12489316331552 19.711994198444284 38.78832091581701 10-11 25.087455716370666 26.56706959895122 27.21861684424724 21.126857840430873 12-13 25.72728718808619 23.60494697953536 27.67761925800487 22.990146574373583 14-15 22.61494242329995 25.118486941345864 25.38855882022209 26.878011815132098 16-17 22.67003823720145 28.316681428349575 25.93641536839581 23.076864966053165 18-19 22.653576849883954 27.1707118820833 27.813079989096018 22.36263127893673 20-21 23.299437374422606 26.303139647998027 28.005876843987142 22.391546133592225 22-23 22.849361381405732 26.047823253415675 27.94868388549554 23.154131479683056 24-25 22.753473913729476 26.211868644954027 27.907000838112246 23.12765660320425 26-27 22.722588486456893 26.506011874062374 27.859550949220267 22.911848690260463 28-29 22.669705100604055 26.594872944904896 27.859720357165635 22.875701597325413 30-31 22.67504546260487 26.426564255163843 27.779764044467285 23.118626237764 32-33 22.64007374540224 26.35764663934812 27.800007251947772 23.202272363301862 34-35 22.939591865982443 26.474126648110673 27.713997544669066 22.872283941237818 36-37 23.043530999544373 26.232700600300312 27.716109083586417 23.007659316568898 38-39 22.85850599605371 26.43799746784845 27.667543523782847 23.035953012314994 40-41 22.945735346568686 26.381987720077333 27.590803062048735 23.081473871305246 42-43 22.992406873559514 26.34155827603946 27.395936742256193 23.27009810814483 44-45 22.927901488292477 26.540893860403692 27.473500740471668 23.057703910832167 46-47 23.111496059661174 26.638203448090543 27.089821336341746 23.16047915590654 48-49 23.166274122576276 26.7026026617457 26.91098826684365 23.220134948834374 50-51 23.123353535968583 26.783935444845014 26.811972768493003 23.280738250693403 52-53 23.255487445004682 26.687991577376113 26.69176863554637 23.364752342072833 54-55 23.158659482058074 26.6949258773856 26.654467067566213 23.491947572990114 56-57 23.03499018772551 26.850408273714166 26.78645717267283 23.328144365887493 58-59 23.120585836387892 26.741184730595595 26.766026962995003 23.37220247002151 60-61 23.090278077544006 26.72616786525196 26.83363046162081 23.34992359558322 62-63 23.098249864429917 26.761215895018847 26.906654939742136 23.233879300809104 64-65 22.976486503647013 26.625186108559113 26.800150830539813 23.598176557254057 66-67 22.89083518153395 26.84123916196478 26.67266339880396 23.595262257697307 68-69 22.954588302812564 26.708940222502736 26.8936415163183 23.442829958366396 70-71 23.01743235858089 26.53569698785351 26.815530740919485 23.631339912646112 72-73 23.010571308546357 26.688056224804185 26.631360576762127 23.670011889887334 74-75 22.850446507344195 26.86465798887528 26.58152699500509 23.70336850877544 76-77 23.062011341933868 26.673563326804672 26.655907991963584 23.608517339297872 78-79 23.063320490070673 26.82137504819866 26.602496757888254 23.512807703842416 80-81 22.98424976174836 26.721263550072987 26.611383437240292 23.683103250938355 82-83 22.98549575722598 26.716542593769077 26.68956357772401 23.608398071280934 84-85 22.812073496308578 26.720958413654206 26.55413129409779 23.91283679593943 86-87 23.014306862748697 26.61152659990404 26.68038783302624 23.693778704321023 88-89 23.01989875848729 26.615767124016482 26.605277564850997 23.75905655264523 90-91 22.934786690742044 26.737396362707106 26.628282324238018 23.699534622312836 92-93 22.97984809639174 26.71598963596036 26.61481625132699 23.689346016320915 94-95 22.928517877032935 26.620230049145775 26.744187175908934 23.707064897912357 96-97 22.98695147350219 26.55121695847633 26.67819379497029 23.78363777305119 98-99 22.67261139073305 26.506785748202123 26.658331966399324 24.162270894665504 100-101 22.718502991246197 26.5224404977482 26.837439722903415 23.92161678810218 102-103 22.67335191882644 26.34527641739206 26.900229615339 24.0811420484425 104-105 22.637939857546556 26.468191865161973 26.809897106404968 24.083971170886507 106-107 22.44747625742515 26.41799320475227 26.75978440995261 24.37474612786997 108-109 22.600329359152557 26.483821256831074 26.678339563674662 24.237509820341703 110-111 22.521509278726107 26.484659847670084 26.68732610684591 24.3065047667579 112-113 22.689867588211303 26.421525519261863 26.719891531522627 24.168715361004207 114-115 22.641073290123735 26.6105934736674 26.544516460439045 24.20381677576982 116-117 22.435185744748814 26.503116609543216 26.756146886412104 24.305550759295873 118-119 22.406385914520794 26.550781242412096 26.68477079649322 24.358062046573888 120-121 22.407069064318083 26.60264248151094 26.715859313705913 24.274429140465063 122-123 22.348896974950215 26.591773354689284 26.640368141935195 24.418961528425307 124-125 22.333373049329424 26.579131747888034 26.60325274007996 24.48424246270258 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 120.0 1 107.0 2 170.5 3 274.0 4 249.0 5 228.0 6 404.0 7 939.5 8 1599.5 9 1957.0 10 1750.5 11 1405.5 12 1425.0 13 1574.0 14 1864.0 15 2242.5 16 2551.5 17 2954.5 18 3500.0 19 4127.5 20 5000.0 21 6192.5 22 7662.5 23 10178.0 24 13703.5 25 17689.0 26 23359.0 27 29971.5 28 36189.5 29 42826.0 30 49859.5 31 58272.5 32 67482.0 33 74191.5 34 80055.0 35 86871.0 36 95253.0 37 103903.5 38 111504.0 39 118658.5 40 126375.5 41 134106.0 42 139610.0 43 145019.5 44 151788.0 45 157470.5 46 162527.0 47 168767.5 48 173048.5 49 175037.5 50 175222.5 51 175642.0 52 177648.5 53 177888.5 54 175119.0 55 165161.0 56 151303.0 57 138418.0 58 128167.0 59 116870.0 60 100499.5 61 83228.5 62 66631.0 63 51373.0 64 38337.0 65 28153.5 66 20641.0 67 15730.5 68 12030.5 69 9305.5 70 6933.0 71 4779.5 72 3472.0 73 2731.0 74 2038.5 75 1388.0 76 955.0 77 577.0 78 377.0 79 234.0 80 149.0 81 102.0 82 54.0 83 35.0 84 27.0 85 22.5 86 15.5 87 8.0 88 3.5 89 4.5 90 3.5 91 2.5 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10646977061408432 2 4.1008020305445005E-4 3 0.0 4 0.17870863585741295 5 0.0 6 8.633267432725263E-5 7 0.038439623244209235 8 0.0 9 0.0 10-11 8.201604061089001E-4 12-13 6.259118888725816E-4 14-15 6.259118888725816E-4 16-17 0.004025260940508154 18-19 0.0015324049693087344 20-21 0.0030432267700356555 22-23 0.0016295292279268935 24-25 0.00451088223359895 26-27 0.004327425300653539 28-29 0.0032698500401446935 30-31 7.877856532361803E-4 32-33 4.3166337163626316E-5 34-35 0.0012086574405815369 36-37 0.0012734069463269763 38-39 5.611623831271421E-4 40-41 0.0014568638792723883 42-43 7.877856532361803E-4 44-45 9.604510018906856E-4 46-47 1.9424851723631845E-4 48-49 5.39579214545329E-5 50-51 0.04129939308129948 52-53 9.712425861815922E-5 54-55 0.13623296008840466 56-57 2.697896072726645E-4 58-59 2.1583168581813158E-5 60-61 0.0 62-63 5.935371359998619E-4 64-65 0.06211635917845827 66-67 0.051465065483333475 68-69 0.0018021945765813987 70-71 0.002848978252799337 72-73 0.07025321373380183 74-75 0.004867004515198867 76-77 0.0017158619022541461 78-79 0.0015431965535996409 80-81 1.8345693294541184E-4 82-83 2.0504010152722502E-4 84-85 1.0791584290906579E-5 86-87 1.9424851723631845E-4 88-89 0.0013273648677815093 90-91 3.021643601453842E-4 92-93 0.002255441116799475 94-95 0.004025260940508154 96-97 0.0024065232968721673 98-99 0.0 100-101 0.0011654911034179106 102-103 0.0012194490248724434 104-105 1.510821800726921E-4 106-107 4.3166337163626316E-5 108-109 0.0 110-111 1.510821800726921E-4 112-113 9.712425861815922E-5 114-115 2.158316858181316E-4 116-117 0.0 118-119 9.280762490179658E-4 120-121 0.00205040101527225 122-123 5.827455517089553E-4 124-125 0.007467776329307353 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4633240.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.12820854671053 #Duplication Level Percentage of deduplicated Percentage of total 1 74.07589237588846 38.61443566053998 2 12.43817259806857 12.967593102641976 3 5.070604327378887 7.929645595063785 4 2.64450670288117 5.51413587643854 5 1.5886089627085744 4.140566965362303 6 0.9746364513329646 3.0483631315386397 7 0.6506751018765012 2.3742969184739264 8 0.43995181022327123 1.8347119771057199 9 0.3524623253590959 1.6535906641059739 >10 1.6383762690540438 14.858673402148636 >50 0.08958973970860609 3.178356176134435 >100 0.035055724888739356 3.0493120718205367 >500 0.0012160205053927647 0.40649807496978163 >1k 1.677267505139275E-4 0.15598038332858877 >5k 8.386337525645515E-5 0.27384000032704303 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6569 0.14177983441393063 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5959 0.12861410157902461 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.1583168581813158E-5 4.3166337163626316E-5 2 0.0 0.0 0.0 4.3166337163626316E-5 4.3166337163626316E-5 3 0.0 0.0 0.0 4.3166337163626316E-5 4.3166337163626316E-5 4 0.0 0.0 0.0 6.474950574543948E-5 6.474950574543948E-5 5 0.0 0.0 0.0 6.474950574543948E-5 6.474950574543948E-5 6 2.1583168581813158E-5 0.0 0.0 6.474950574543948E-5 6.474950574543948E-5 7 2.1583168581813158E-5 0.0 0.0 8.633267432725263E-5 6.474950574543948E-5 8 2.1583168581813158E-5 0.0 0.0 8.633267432725263E-5 6.474950574543948E-5 9 2.1583168581813158E-5 0.0 0.0 1.079158429090658E-4 6.474950574543948E-5 10-11 2.1583168581813158E-5 0.0 0.0 1.2949901149087896E-4 6.474950574543948E-5 12-13 2.1583168581813158E-5 0.0 0.0 1.7266534865450527E-4 8.633267432725263E-5 14-15 2.1583168581813158E-5 0.0 0.0 2.0504010152722502E-4 8.633267432725263E-5 16-17 2.1583168581813158E-5 0.0 0.0 3.129559444362908E-4 1.079158429090658E-4 18-19 3.237475287271974E-5 0.0 0.0 3.992886187635434E-4 1.2949901149087896E-4 20-21 4.3166337163626316E-5 0.0 0.0 4.964128773817027E-4 1.510821800726921E-4 22-23 4.3166337163626316E-5 0.0 0.0 7.877856532361803E-4 1.9424851723631845E-4 24-25 4.3166337163626316E-5 0.0 0.0 0.001079158429090658 2.2662327010903818E-4 26-27 4.3166337163626316E-5 0.0 0.0 0.00123024060916335 2.589980229817579E-4 28-29 4.3166337163626316E-5 0.0 0.0 0.0016187376436359868 2.8058119156357107E-4 30-31 4.3166337163626316E-5 0.0 0.0 0.003161934197235628 3.237475287271974E-4 32-33 4.3166337163626316E-5 0.0 0.0 0.006960571867634743 4.5324654021807636E-4 34-35 4.3166337163626316E-5 0.0 0.0 0.012971484317669708 4.5324654021807636E-4 36-37 4.3166337163626316E-5 0.0 0.0 0.022986074539631016 4.5324654021807636E-4 38-39 4.3166337163626316E-5 0.0 0.0 0.040025986134972505 4.748297087998895E-4 40-41 5.39579214545329E-5 0.0 0.0 0.05913788191416805 4.964128773817027E-4 42-43 6.474950574543948E-5 0.0 0.0 0.07942606038107242 4.964128773817027E-4 44-45 6.474950574543948E-5 0.0 0.0 0.10380424929423039 5.179960459635158E-4 46-47 6.474950574543948E-5 0.0 0.0 0.13517538482789582 5.503707988362356E-4 48-49 6.474950574543948E-5 0.0 0.0 0.16897462682701522 6.043287202907684E-4 50-51 6.474950574543948E-5 0.0 0.0 0.2066804223394428 6.259118888725816E-4 52-53 6.474950574543948E-5 0.0 0.0 0.24564883321390646 7.44619316072554E-4 54-55 6.474950574543948E-5 0.0 0.0 0.2877036371955694 8.201604061089E-4 56-57 6.474950574543948E-5 0.0 0.0 0.33828379276704856 8.201604061089E-4 58-59 6.474950574543948E-5 0.0 0.0 0.3967849712080531 8.525351589816197E-4 60-61 6.474950574543948E-5 0.0 0.0 0.45958120019683846 8.849099118543396E-4 62-63 6.474950574543948E-5 0.0 0.0 0.5273955158808954 9.280762490179659E-4 64-65 6.474950574543948E-5 0.0 0.0 0.5977026875361517 9.388678333088724E-4 66-67 6.474950574543948E-5 0.0 0.0 0.6796000207198418 9.820341704724986E-4 68-69 6.474950574543948E-5 0.0 0.0 0.7748573352556742 9.928257547634053E-4 70-71 6.474950574543948E-5 0.0 0.0 0.8828163445019037 0.001025200507636125 72-73 6.474950574543948E-5 0.0 0.0 0.9977683003686406 0.0011654911034179106 74-75 6.474950574543948E-5 0.0 0.0 1.112655506729632 0.0011870742719997237 76-77 6.474950574543948E-5 0.0 0.0 1.2435034662568742 0.0012086574405815369 78-79 8.633267432725263E-5 2.1583168581813158E-5 0.0 1.393355405720403 0.0012194490248724436 80-81 8.633267432725263E-5 2.1583168581813158E-5 0.0 1.5644020167312722 0.0012518237777451632 82-83 8.633267432725263E-5 2.1583168581813158E-5 0.0 1.7500280581191565 0.0012949901149087895 84-85 8.633267432725263E-5 2.1583168581813158E-5 0.0 1.9378339995338036 0.0012949901149087895 86-87 1.079158429090658E-4 2.1583168581813158E-5 0.0 2.1399927480553567 0.0013705312049451354 88-89 1.079158429090658E-4 2.1583168581813158E-5 0.0 2.364986488936468 0.0014136975421087617 90-91 1.079158429090658E-4 2.1583168581813158E-5 0.0 2.6244809247956074 0.0014244891263996684 92-93 1.079158429090658E-4 2.1583168581813158E-5 0.0 2.903238338614015 0.0014460722949814816 94-95 1.079158429090658E-4 2.1583168581813158E-5 0.0 3.1858807227771493 0.0014784470478542014 96-97 1.079158429090658E-4 2.1583168581813158E-5 0.0 3.4740807728501006 0.0015755713064723605 98-99 1.079158429090658E-4 2.1583168581813158E-5 0.0 3.7942994535141716 0.0016403208122178 100-101 1.079158429090658E-4 2.1583168581813158E-5 0.0 4.162864431801504 0.0016403208122178 102-103 1.079158429090658E-4 2.1583168581813158E-5 0.0 4.557922749523012 0.0016834871493814263 104-105 1.079158429090658E-4 2.1583168581813158E-5 0.0 4.953045816750265 0.0017050703179632394 106-107 1.079158429090658E-4 2.1583168581813158E-5 0.0 5.358237432120935 0.0017158619022541461 108-109 1.079158429090658E-4 2.1583168581813158E-5 0.0 5.788487106215089 0.0017698198237086791 110-111 1.079158429090658E-4 2.1583168581813158E-5 0.0 6.2600685481434155 0.0018669440823268382 112-113 1.079158429090658E-4 4.3166337163626316E-5 0.0 6.762276506289336 0.001877735666617745 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6375 0.0 62.399185 1 GTATCAA 11415 0.0 48.32803 1 TCAACGC 13790 0.0 37.72025 4 ATCAACG 13965 0.0 37.41823 3 TATCAAC 14925 0.0 36.807907 2 CAACGCA 14135 0.0 36.612167 5 AACGCAG 14790 0.0 34.34723 6 ACGCAGA 17585 0.0 28.78683 7 CGCAGAG 17885 0.0 28.429947 8 GTACATG 14105 0.0 25.411755 1 TACATGG 13540 0.0 25.171083 2 GCAGAGT 20425 0.0 23.8754 9 AGAGTAC 17380 0.0 23.595047 10-11 GAGTACT 12100 0.0 23.519762 12-13 ACATGGG 13865 0.0 23.2059 3 CAGAGTA 18865 0.0 22.762333 10-11 GTACTTT 13330 0.0 21.572844 14-15 GTGGTAT 3930 0.0 21.549097 1 TGGTATC 3540 0.0 21.0393 2 AGTACTT 13275 0.0 20.676334 12-13 >>END_MODULE