FastQCFastQC Report
Thu 26 May 2016
SRR1364503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541582
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.136135682421421No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31190.12271884204404973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG138400.034.4079741
TACATGG138950.032.2418022
GTATCAA128600.031.7069761
ACATGGG140950.029.6749673
CATGGGA100500.026.8543034
ATCAACG147050.026.623243
CAACGCA148200.026.6128585
TCAACGC147550.026.5282064
TATCAAC153650.026.3692282
AACGCAG147350.026.1464886
GAGTACT50500.023.67473212-13
ACGCAGA163150.023.140357
CGCATAG3353.45608E-1123.1104879
CGCAGAG159750.023.0014298
GGTATCA95550.022.6764931
ATGGGAG42050.022.385815
CATGGGG47750.021.460354
AACGCAT5000.021.4368866
ACGCATA4251.8189894E-1221.019048
GCAGAGT166450.020.6444159
Produced by FastQC (version 0.11.5)