##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364503_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2541582 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.616780808173807 30.0 25.0 31.0 7.0 34.0 2 25.801785266027224 30.0 25.0 31.0 7.0 34.0 3 25.82825342640922 30.0 25.0 31.0 7.0 34.0 4 28.552626277649118 35.0 26.0 37.0 7.0 37.0 5 29.180044948382545 35.0 28.0 37.0 2.0 37.0 6 29.356252916490597 35.0 29.0 37.0 2.0 37.0 7 29.099043823886067 35.0 28.0 37.0 2.0 37.0 8 28.72933196725504 35.0 27.0 37.0 2.0 37.0 9 29.309853862672934 35.0 27.0 39.0 2.0 39.0 10-11 29.399107130912952 35.0 27.0 39.0 2.0 39.0 12-13 29.244369845238126 35.0 27.0 39.0 2.0 39.0 14-15 29.852075006826457 36.0 27.0 40.0 2.0 41.0 16-17 30.11328633111188 36.0 27.0 40.0 2.0 41.0 18-19 30.086160116022228 36.0 27.0 40.0 2.0 41.0 20-21 29.893111259050464 36.0 26.5 40.0 2.0 41.0 22-23 29.942600907623678 36.0 27.0 40.0 2.0 41.0 24-25 29.966312123708775 36.0 27.0 40.0 2.0 41.0 26-27 30.083614654179954 36.0 27.0 40.0 2.0 41.0 28-29 30.019595275698364 36.0 27.0 40.0 2.0 41.0 30-31 29.679128786716305 36.0 26.0 40.0 2.0 41.0 32-33 29.689466049098556 36.0 26.5 40.0 2.0 41.0 34-35 29.392923187211743 35.0 25.0 40.0 2.0 41.0 36-37 29.135203979253866 35.0 25.0 40.0 2.0 41.0 38-39 28.985174784838733 35.0 25.0 40.0 2.0 41.0 40-41 29.016404153003915 35.0 25.0 40.0 2.0 41.0 42-43 28.877449950463923 35.0 24.5 40.0 2.0 41.0 44-45 28.527806893501765 35.0 24.0 39.5 2.0 41.0 46-47 28.277001883079123 34.5 23.5 39.0 2.0 41.0 48-49 28.020490584210936 34.0 22.5 39.0 2.0 41.0 50-51 27.99917079204999 34.0 22.5 39.0 2.0 41.0 52-53 27.809560934882292 34.0 22.0 39.0 2.0 40.5 54-55 27.70335641344643 34.0 21.0 39.0 2.0 40.0 56-57 27.293325771114212 33.0 19.0 39.0 2.0 40.0 58-59 27.079476483544504 33.0 18.0 39.0 2.0 40.0 60-61 26.670998417521055 33.0 14.5 38.0 2.0 40.0 62-63 26.633354343869293 33.0 16.0 38.0 2.0 40.0 64-65 26.4664169796607 33.0 15.0 38.0 2.0 40.0 66-67 26.237275641706624 32.5 13.0 37.5 2.0 40.0 68-69 26.085070243651394 32.0 13.0 37.0 2.0 40.0 70-71 25.94371851862344 32.0 12.0 37.0 2.0 40.0 72-73 25.914679125048885 32.0 12.0 36.5 2.0 39.0 74-75 25.636719177268333 32.0 9.5 36.0 2.0 39.0 76-77 25.340027589115756 32.0 8.0 36.0 2.0 39.0 78-79 25.017366742446242 32.0 7.0 35.0 2.0 38.0 80-81 25.004051216919226 32.0 7.0 35.0 2.0 37.0 82-83 24.785147793775685 32.0 4.0 35.0 2.0 37.0 84-85 24.575556680838943 32.0 2.0 35.0 2.0 36.0 86-87 24.34127012230965 32.0 2.0 35.0 2.0 36.0 88-89 24.091164872901995 32.0 2.0 35.0 2.0 36.0 90-91 23.9002398899583 32.0 2.0 35.0 2.0 35.5 92-93 23.781614561324403 32.0 2.0 35.0 2.0 35.0 94-95 23.70461016012861 32.0 2.0 35.0 2.0 35.0 96-97 23.554654148479173 32.0 2.0 35.0 2.0 35.0 98-99 23.447032006049774 31.0 2.0 35.0 2.0 35.0 100-101 23.379338537965722 31.0 2.0 35.0 2.0 35.0 102-103 22.269922827593206 29.5 2.0 33.5 2.0 35.0 104-105 23.005754880228142 31.0 2.0 34.0 2.0 35.0 106-107 23.081340676791072 31.0 2.0 35.0 2.0 35.0 108-109 23.02189679498832 31.0 2.0 35.0 2.0 35.0 110-111 22.907593577543437 31.0 2.0 35.0 2.0 35.0 112-113 22.8771306611394 31.0 2.0 35.0 2.0 35.0 114-115 22.816447000332865 31.0 2.0 35.0 2.0 35.0 116-117 22.610101306981242 31.0 2.0 35.0 2.0 35.0 118-119 22.45943412410066 31.0 2.0 35.0 2.0 35.0 120-121 22.305794973366982 31.0 2.0 35.0 2.0 35.0 122-123 22.03072417100845 31.0 2.0 35.0 2.0 35.0 124-125 21.076055582704 29.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.9856630092062915 1101 2 1.609966527306387 1101 3 1.8829677233437572 1101 4 2.3042110113011347 1101 5 1.9442050009115235 1101 6 1.8247309821767175 1101 7 1.6989631035455588 1101 8 2.056341836454756 1101 9 2.59316769652213 1101 10-11 2.5796286283032863 1101 12-13 2.3587078758356874 1101 14-15 2.654582174154509 1101 16-17 2.187356971269068 1101 18-19 2.2327234998381194 1101 20-21 2.603698300699346 1101 22-23 2.454288833786247 1101 24-25 2.369367475635677 1101 26-27 2.052339602987768 1101 28-29 1.9667407202792333 1101 30-31 2.1161115362229914 1101 32-33 2.3741396531950585 1101 34-35 2.583341657635124 1101 36-37 2.502801568295059 1101 38-39 2.632305001298171 1101 40-41 2.496517197799154 1101 42-43 2.375704465459428 1101 44-45 2.6688196784555096 1101 46-47 2.543782833805434 1101 48-49 2.5513661229810083 1101 50-51 2.5421470918383235 1101 52-53 2.6466180902591745 1101 54-55 2.5178332980639233 1101 56-57 2.718810047578426 1101 58-59 2.7557276651147653 1101 60-61 2.8397579271449267 1101 62-63 2.418888070919646 1101 64-65 2.5618671608733976 1101 66-67 2.697071465897114 1101 68-69 2.5648998447559563 1101 70-71 2.5326359735089277 1101 72-73 2.318106041515822 1101 74-75 2.3567173143326556 1101 76-77 2.566372120106081 1101 78-79 2.5452280218793213 1101 80-81 2.355318686277002 1101 82-83 2.246286557154889 1101 84-85 2.204784583562393 1101 86-87 2.3094706173406543 1101 88-89 2.3863210912171375 1101 90-91 2.380864181602984 1101 92-93 2.4169716910329946 1101 94-95 2.3540303783962635 1101 96-97 2.3287302578262867 1101 98-99 2.2249690080566857 1101 100-101 2.2295266701381173 1101 102-103 2.2864070619147867 1101 104-105 2.317001930383636 1101 106-107 2.3798781392643846 1101 108-109 2.4392929855892085 1101 110-111 2.579260320618353 1101 112-113 2.4738744414131304 1101 114-115 2.4496087251779493 1101 116-117 2.4903752103582093 1101 118-119 2.5306962564503337 1101 120-121 2.5116419542832666 1101 122-123 2.566928598068184 1101 124-125 2.5034683129801856 1102 1 0.908956184563376 1102 2 0.5593747802949984 1102 3 0.6403725611560809 1102 4 1.108653661701009 1102 5 1.1506558599505219 1102 6 1.0940889370013167 1102 7 1.0487264180192923 1102 8 1.113534088837877 1102 9 1.4974984109339893 1102 10-11 1.2510492327576195 1102 12-13 1.5324535753293844 1102 14-15 1.6920382396111684 1102 16-17 1.252387597645864 1102 18-19 1.5021297876397561 1102 20-21 1.632570748113995 1102 22-23 1.5157213754236487 1102 24-25 1.2173013072589924 1102 26-27 1.1623094081554122 1102 28-29 0.9611161028599753 1102 30-31 0.9058838666559055 1102 32-33 0.9187614144540568 1102 34-35 1.3114365283643252 1102 36-37 1.4532843669337687 1102 38-39 1.5254189462915235 1102 40-41 1.0707652150184437 1102 42-43 1.0565935532276 1102 44-45 1.4688145663137107 1102 46-47 1.2937612565835437 1102 48-49 1.413299256166244 1102 50-51 1.3456220204343197 1102 52-53 1.4305147108014857 1102 54-55 1.2078468950332848 1102 56-57 1.3581225508150077 1102 58-59 1.4252796157620153 1102 60-61 1.9100393870664014 1102 62-63 1.760776628884031 1102 64-65 1.8682868823214243 1102 66-67 1.8348059771033292 1102 68-69 1.62882850581299 1102 70-71 1.8289290067883037 1102 72-73 1.7443047059853747 1102 74-75 1.7784442489463714 1102 76-77 1.7280932653851835 1102 78-79 1.5811330502351097 1102 80-81 1.1789711721721368 1102 82-83 1.1835071985228716 1102 84-85 1.2514683305366034 1102 86-87 1.387982227213861 1102 88-89 1.136208225748792 1102 90-91 1.0726415338853243 1102 92-93 1.0625543357763192 1102 94-95 1.2709894772783628 1102 96-97 1.3677479830161303 1102 98-99 1.4091438751741983 1102 100-101 1.3624560336167093 1102 102-103 1.0975970091866962 1102 104-105 1.10922291034802 1102 106-107 1.4413001246609234 1102 108-109 1.512882864345137 1102 110-111 1.2804433499009669 1102 112-113 1.2286763359596016 1102 114-115 1.2845782315840601 1102 116-117 1.5172711842477753 1102 118-119 1.5547860628395824 1102 120-121 1.5694722949112503 1102 122-123 1.6210487187943983 1102 124-125 1.4368819357094331 1103 1 1.7890740793346822 1103 2 1.5045800228379242 1103 3 1.6619777793548778 1103 4 2.1569201919138052 1103 5 1.5848156791702657 1103 6 1.507665159808731 1103 7 1.5188239305217337 1103 8 1.908106984658069 1103 9 2.4546102966390855 1103 10-11 2.0630335289448993 1103 12-13 2.2063960215264125 1103 14-15 2.4032311007079663 1103 16-17 2.2156078271434296 1103 18-19 1.9685852188034936 1103 20-21 2.4104731289409607 1103 22-23 2.33776855049452 1103 24-25 2.2740267628896333 1103 26-27 2.0650597536796944 1103 28-29 1.910201884369286 1103 30-31 2.0750345192013917 1103 32-33 2.0505957750859523 1103 34-35 1.9271264989916261 1103 36-37 2.2848056863328594 1103 38-39 2.237243744855064 1103 40-41 2.394925306195397 1103 42-43 2.3046984669267303 1103 44-45 2.2209412122984276 1103 46-47 2.4388212858572302 1103 48-49 2.5653081222353684 1103 50-51 2.5003654096627805 1103 52-53 2.4393229560160634 1103 54-55 2.3517550667005978 1103 56-57 2.493490044878488 1103 58-59 2.6912761785397095 1103 60-61 2.5326396452145197 1103 62-63 2.3202162768921895 1103 64-65 2.281869640022993 1103 66-67 2.3591363451789285 1103 68-69 2.2497474149054852 1103 70-71 2.3955713623285284 1103 72-73 2.371000527521243 1103 74-75 2.370474008194268 1103 76-77 2.4748303729301355 1103 78-79 2.5583573268784363 1103 80-81 2.035300467997029 1103 82-83 2.231042909739166 1103 84-85 2.1227757027817304 1103 86-87 2.140520010453667 1103 88-89 2.170653685852205 1103 90-91 2.0894403044275016 1103 92-93 2.092472236382825 1103 94-95 1.9304806651386173 1103 96-97 2.1209593224172814 1103 98-99 2.023159922761959 1103 100-101 1.7869358263377855 1103 102-103 1.8512214001048584 1103 104-105 2.0515113457900647 1103 106-107 1.7902805689571544 1103 108-109 2.094226619448463 1103 110-111 2.2533275326979307 1103 112-113 2.140873333050319 1103 114-115 2.0186220993383976 1103 116-117 2.0937165741175825 1103 118-119 2.2376598280911324 1103 120-121 2.2032159997948995 1103 122-123 2.166275566093457 1103 124-125 2.098387917249873 1104 1 1.321582754344906 1104 2 1.101961397109534 1104 3 1.2080370022314 1104 4 1.392703784941812 1104 5 0.8679869850288924 1104 6 0.9657194816940446 1104 7 0.9181227902888445 1104 8 1.2331356882110498 1104 9 1.801800613953187 1104 10-11 1.6437206846155625 1104 12-13 1.1410406667601798 1104 14-15 1.5349978849452803 1104 16-17 1.3094125717131533 1104 18-19 0.6460734384580924 1104 20-21 -3.714160541459684 1104 22-23 -0.6761997462806626 1104 24-25 0.8360203843498937 1104 26-27 0.9581312430647451 1104 28-29 0.5761033919778527 1104 30-31 1.3732647140602445 1104 32-33 1.1200679028713019 1104 34-35 1.3861525884011918 1104 36-37 1.49543178075864 1104 38-39 1.5859738788116005 1104 40-41 1.6274207018934455 1104 42-43 1.6186694270141722 1104 44-45 1.7015928695553448 1104 46-47 1.5977519926804362 1104 48-49 1.7025312821007823 1104 50-51 1.6378780262509807 1104 52-53 1.5813340249653045 1104 54-55 1.5300218240564618 1104 56-57 1.8629438523037543 1104 58-59 1.839152183909043 1104 60-61 1.9861366300842995 1104 62-63 1.579826891948457 1104 64-65 1.669210972729548 1104 66-67 1.6364670817517712 1104 68-69 1.6879858554784164 1104 70-71 1.8925642406688006 1104 72-73 1.7156701346336405 1104 74-75 1.8003976686009722 1104 76-77 1.6185247297253262 1104 78-79 1.6176151244545345 1104 80-81 1.4329598697190065 1104 82-83 1.3852246638524939 1104 84-85 1.3647775401567799 1104 86-87 1.2988902731878689 1104 88-89 1.4403214708616936 1104 90-91 1.4803613398885425 1104 92-93 1.1017113535239886 1104 94-95 1.291082001314308 1104 96-97 1.3766945387682554 1104 98-99 1.3062432275686682 1104 100-101 0.7735764285518023 1104 102-103 1.2212536243207346 1104 104-105 1.4423964048069564 1104 106-107 1.5469435367859568 1104 108-109 1.6046871353165244 1104 110-111 1.5818710013684978 1104 112-113 1.5838516002981216 1104 114-115 1.4714233637226108 1104 116-117 1.5792296453934078 1104 118-119 1.541511443231709 1104 120-121 1.5597076402847492 1104 122-123 1.3611701144484698 1104 124-125 1.4767863564619788 1105 1 1.32134031644242 1105 2 1.0007224656612514 1105 3 1.067090139338184 1105 4 1.4844100283097958 1105 5 1.2348189012379898 1105 6 1.2405602543562217 1105 7 1.0855426199611848 1105 8 1.3657762479543578 1105 9 1.8445943441160182 1105 10-11 1.596364739283171 1105 12-13 1.608207687816389 1105 14-15 1.8709656151029073 1105 16-17 1.511702887722997 1105 18-19 1.1916923667399892 1105 20-21 1.5020653357830547 1105 22-23 1.2783568155171956 1105 24-25 1.2792017981961692 1105 26-27 1.2316556851569267 1105 28-29 0.8972991470395755 1105 30-31 1.3626177786526164 1105 32-33 1.2722945130687222 1105 34-35 1.1637177655159974 1105 36-37 1.4037195244843588 1105 38-39 1.2415507006323239 1105 40-41 1.3523068379043934 1105 42-43 1.6165224785269245 1105 44-45 1.7875017104244613 1105 46-47 2.1613205620662157 1105 48-49 1.7344927925577203 1105 50-51 1.7913214131264574 1105 52-53 1.7487615102451528 1105 54-55 1.7283874479364023 1105 56-57 2.1791112059914575 1105 58-59 1.877814830482933 1105 60-61 2.2461652279625746 1105 62-63 2.0464808633242946 1105 64-65 1.7597495344894725 1105 66-67 1.8708771654328302 1105 68-69 1.4110384889888046 1105 70-71 1.5584884063904454 1105 72-73 1.5636241905744974 1105 74-75 1.8440672374557536 1105 76-77 1.7835812082723308 1105 78-79 1.590775649340344 1105 80-81 1.4916337474100452 1105 82-83 1.621014275608431 1105 84-85 1.6138827019207014 1105 86-87 1.5544234487474995 1105 88-89 1.7391741029587386 1105 90-91 1.7548923548931619 1105 92-93 1.7335936361918272 1105 94-95 1.5384375003278805 1105 96-97 1.653841415968465 1105 98-99 1.4833283082091846 1105 100-101 1.6281622862209773 1105 102-103 1.6658748192823403 1105 104-105 1.7618412573438356 1105 106-107 1.9262796325848193 1105 108-109 1.9647868590575754 1105 110-111 1.7836098966773548 1105 112-113 1.947073944059568 1105 114-115 1.916481331238785 1105 116-117 2.03464301534472 1105 118-119 1.997315745075639 1105 120-121 2.0186730571471436 1105 122-123 2.0751108703364984 1105 124-125 1.8093263580960013 1106 1 -0.41361235633824833 1106 2 -0.22673177229864194 1106 3 -0.34489836649819594 1106 4 -0.6685539890136489 1106 5 -0.9931365349828418 1106 6 -0.3697698085937482 1106 7 -0.32094116458000244 1106 8 -0.034543031562566995 1106 9 0.02915045537306682 1106 10-11 -2.4845692786191833 1106 12-13 -0.73272016595973 1106 14-15 -0.36168728429967345 1106 16-17 -0.6743901831337098 1106 18-19 -3.3911545568060966 1106 20-21 -5.746762902581764 1106 22-23 -6.618185295269274 1106 24-25 -6.35991411559672 1106 26-27 -5.241005556396839 1106 28-29 -2.065976795652233 1106 30-31 -3.963274600779023 1106 32-33 -4.5017623879275455 1106 34-35 -3.820037987863145 1106 36-37 -5.99766364130204 1106 38-39 -6.521956478863888 1106 40-41 -2.7730962308633025 1106 42-43 -1.4107982559737913 1106 44-45 -0.6380427910758186 1106 46-47 -0.45374739957200916 1106 48-49 -0.20310994945572602 1106 50-51 -0.32282982097088464 1106 52-53 -0.5103628517592611 1106 54-55 -0.45111005835130413 1106 56-57 -0.31790306903652876 1106 58-59 -0.08903932309585372 1106 60-61 0.15052057708766142 1106 62-63 -0.11004656062964813 1106 64-65 -0.3139956437894469 1106 66-67 -0.5795344149932049 1106 68-69 -0.3929815326790731 1106 70-71 -0.13128079177882412 1106 72-73 -0.03380738771896574 1106 74-75 -0.1731925054022021 1106 76-77 -0.28315274989532213 1106 78-79 -0.22934392233495515 1106 80-81 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2216 80-81 -3.8360332788964264 2216 82-83 -4.0964528680291075 2216 84-85 -3.7818444746936493 2216 86-87 -4.048486536905617 2216 88-89 -4.324713921917212 2216 90-91 -4.355927383859104 2216 92-93 -4.081142019620195 2216 94-95 -7.253920657084777 2216 96-97 -9.213641321588987 2216 98-99 -6.1389439543075035 2216 100-101 -5.176892454482537 2216 102-103 -6.135696167653485 2216 104-105 -8.823557354752626 2216 106-107 -5.554197774216274 2216 108-109 -7.817565790309814 2216 110-111 -5.285831403871647 2216 112-113 -4.686616301554942 2216 114-115 -5.0146775718473116 2216 116-117 -4.627861709431379 2216 118-119 -4.960125895639376 2216 120-121 -5.742619075840857 2216 122-123 -8.596323837590303 2216 124-125 -7.088290580075196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 270085.0 3 39449.0 4 22871.0 5 19264.0 6 19296.0 7 20894.0 8 21954.0 9 23557.0 10 25382.0 11 25772.0 12 26509.0 13 27362.0 14 29054.0 15 25425.0 16 20704.0 17 18454.0 18 16986.0 19 15619.0 20 15650.0 21 16971.0 22 20568.0 23 27975.0 24 37023.0 25 46431.0 26 56226.0 27 62196.0 28 70499.0 29 79398.0 30 88231.0 31 97691.0 32 115788.0 33 137905.0 34 167440.0 35 207337.0 36 262721.0 37 277241.0 38 85314.0 39 340.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.460338878379595 19.606321014027834 13.95993655827458 22.973403549317986 2 14.475637652889493 21.497258261818285 40.8674062983622 23.159697786930025 3 18.48475496706468 27.40724616345081 29.209339821463576 24.898659048020928 4 10.764078064327173 17.385437104175 38.85533157183663 32.9951532596612 5 12.749078798185941 39.71068751574704 33.913099962207106 13.627133723859913 6 29.717514168753357 38.07597693967518 18.451879750132598 13.754629141438862 7 27.29024348210653 33.952812898626114 21.337282641797774 17.419660977469583 8 25.04919278970038 36.26219786420075 20.474469915967973 18.214139430130892 9 26.10230234578627 17.41267045669172 20.924778705347588 35.560248492174416 10-11 23.52588570877372 28.40724984891227 27.734451743463858 20.33241269885016 12-13 24.749164340197645 25.899964783259655 28.082202561181706 21.26866831536099 14-15 22.068173894429 27.118747112659598 26.17644119112285 24.63663780178856 16-17 21.370165019644823 30.71101324065068 26.434469414704953 21.48435232499954 18-19 21.148801689376054 29.320520814865787 28.73908942992806 20.791588065830098 20-21 22.379769197992474 28.292245984722335 28.526609642182226 20.80137517510296 22-23 21.849471818688215 28.39999441192495 28.087652498863203 21.66288127052363 24-25 21.539741699319244 28.34289004138682 28.537526557889016 21.579841701404916 26-27 21.68500735522831 28.597269328452015 28.21789025255672 21.499833063762953 28-29 21.32760564322406 28.55755856389352 28.57418289916603 21.540652893716395 30-31 21.411208345252067 28.427608392940122 28.722019172014196 21.439164089793618 32-33 21.352021975280564 28.497785276703674 28.27967723314817 21.870515514867588 34-35 21.685138722922304 28.220148003386736 28.382401177762066 21.712312095928894 36-37 21.81595836957332 28.09571803180515 28.34771423956618 21.740609359055348 38-39 21.691160267952707 28.211135525139454 28.532205793469913 21.56549841343793 40-41 21.65630327526725 28.13616978736041 28.690198128154453 21.517328809217894 42-43 21.521771852277432 28.30170599710961 28.347531119862506 21.828991030750448 44-45 21.513991696034118 28.444815417858155 28.13614943120729 21.905043454900436 46-47 21.55123518009153 28.512458235317613 27.71012777440156 22.226178810189293 48-49 21.683477874899584 28.50454519430034 27.735827636961325 22.07614929383875 50-51 21.897706528698198 28.60609926504875 27.528797756385913 21.96739644986713 52-53 21.871736309124206 28.654074542207713 27.61118880788661 21.86300034078147 54-55 21.648541350472144 28.493079693021926 27.731694427512966 22.126684528992964 56-57 21.806345118492644 28.85357592903918 27.414075495715455 21.926003456752717 58-59 21.73400693938057 28.31715102105386 27.766474929254443 22.182367110311123 60-61 21.85479141493059 28.398053284737614 27.84021269812531 21.906942602206485 62-63 22.01290330704299 28.406281036482774 27.644524751275302 21.93629090519894 64-65 21.76684657622593 28.268931377353823 27.763554256868193 22.20066778955205 66-67 21.64740468382849 28.649832754139236 27.568169829952442 22.134592732079824 68-69 21.721863894341467 28.556914388044337 27.700619728086163 22.020601989528032 70-71 21.397888852120886 28.491348086789447 27.987641754093413 22.123121306996254 72-73 21.7177758174723 28.41694490850873 27.569018797161103 22.29626047685786 74-75 21.637246276678624 28.505599736853288 27.531429664125955 22.32572432234213 76-77 21.76982960251079 28.313984052583034 27.812243246901648 22.103943098004528 78-79 21.624518425044513 28.34113501650819 27.87807139007439 22.15627516837291 80-81 21.46233556058282 28.918024195000108 27.56841448992918 22.051225754487895 82-83 21.703010891463105 28.753974124905568 27.421402826743897 22.121612156887434 84-85 21.728253607512407 28.410935738000596 27.37263240398788 22.488178250499114 86-87 21.7823192448306 28.33388321205507 27.609971317775013 22.273826225339317 88-89 21.995941225482333 28.114715959561774 27.652137644199133 22.237205170756756 90-91 21.70078791588193 28.417481112663374 27.87446465140098 22.007266320053716 92-93 21.725308630438473 28.443498462823584 27.703367741349094 22.127825165388852 94-95 21.552026365833015 28.36929872502972 27.60926644451186 22.469408464625406 96-97 21.793630118135138 28.233253204702585 27.47361489900704 22.49950177815524 98-99 21.65755817007454 28.12419400182924 27.50457752858902 22.7136702995072 100-101 21.583130254163247 28.256403264769396 27.72163959305079 22.438826888016568 102-103 21.73909365489968 28.002531516574763 27.91581310608703 22.342561722438525 104-105 21.61175922963935 28.0006287726853 27.91298226727246 22.47462973040289 106-107 21.446045233251475 28.336165256017026 27.814792453216224 22.40299705751528 108-109 21.464775532029165 28.274536374163254 27.50034132808298 22.760346765724602 110-111 21.26170016954401 28.277257376777627 27.76048062519993 22.700561828478435 112-113 21.593004549907032 28.19154032462575 27.723132245161825 22.492322880305395 114-115 21.302212849003745 28.444513519468668 27.576824105259846 22.676449526267746 116-117 21.138681472871664 28.396309675906377 27.804759238499805 22.66024961272215 118-119 21.331116000191226 28.49106518750899 27.46224018455305 22.715578627746737 120-121 21.076339935838742 28.615248470435102 27.56305655642205 22.745355037304112 122-123 21.2597337725536 28.506671425061093 27.41195216082324 22.82164264156207 124-125 21.38502505168004 28.511890444221972 27.299363147594885 22.803721356503104 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2463.0 1 2507.5 2 3061.5 3 3217.0 4 2430.5 5 1698.0 6 1324.5 7 1373.0 8 1693.5 9 1838.0 10 1647.0 11 1493.0 12 1579.0 13 1699.0 14 1807.0 15 2038.5 16 2275.0 17 2511.0 18 2794.5 19 3191.0 20 3882.5 21 4865.5 22 6123.0 23 7781.5 24 9857.5 25 12634.0 26 16728.0 27 21235.0 28 25408.5 29 30190.0 30 35811.5 31 41908.0 32 48840.0 33 55592.0 34 62292.5 35 68100.0 36 73998.5 37 80683.0 38 83676.0 39 83547.0 40 83558.0 41 84961.0 42 86374.0 43 88260.5 44 90308.0 45 90969.5 46 89562.5 47 88974.5 48 94413.0 49 94949.5 50 89116.0 51 84167.5 52 78882.0 53 74387.5 54 69675.0 55 63864.0 56 57508.0 57 51214.5 58 45840.0 59 41413.5 60 36319.0 61 30334.0 62 24718.5 63 19630.0 64 15285.5 65 12051.5 66 9315.5 67 7219.5 68 5435.0 69 3918.0 70 3019.5 71 2490.5 72 2026.5 73 1695.0 74 1382.5 75 1096.5 76 802.0 77 522.0 78 361.5 79 244.0 80 180.0 81 137.0 82 97.0 83 72.0 84 52.5 85 40.5 86 37.5 87 29.0 88 23.0 89 19.0 90 15.5 91 11.0 92 8.0 93 5.5 94 2.5 95 3.5 96 3.0 97 2.5 98 2.0 99 2.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1006853211897157 2 0.033758501594676076 3 0.04516871775138477 4 0.05185746515359331 5 0.055949404740826776 6 0.0029115723986084256 7 0.0015738229181667166 8 0.0020066242206625636 9 0.006846129694025218 10-11 0.06558907011459791 12-13 0.006964166412887721 14-15 0.01243320105351706 16-17 0.02287945067284864 18-19 0.02067609858741524 20-21 0.13505367916518138 22-23 0.017961254053577656 24-25 0.017115324235063043 26-27 0.007574022793677323 28-29 0.0049968877651793255 30-31 0.0025574622420209145 32-33 0.007121548704704393 34-35 0.019338349106973532 36-37 0.0029705907580396774 38-39 0.009364246363091964 40-41 0.03255846161957395 42-43 0.015718556395190084 44-45 0.11471201794787655 46-47 0.004347685811435555 48-49 0.01068232305705659 50-51 0.012787311210104573 52-53 0.014321788555317122 54-55 0.0115282528755712 56-57 0.00706253034527314 58-59 0.017036633089154707 60-61 0.03509625107511778 62-63 0.008577334904008605 64-65 0.00836093425276068 66-67 0.010367558473423246 68-69 0.008872426701164865 70-71 0.0047608143274543175 72-73 0.0011016760427167017 74-75 0.0013377494804417093 76-77 0.00904948177945862 78-79 0.022446649370352796 80-81 0.004190303519618884 82-83 0.005587071359491844 84-85 0.003147645836333433 86-87 0.00442637695734389 88-89 0.006865802480502302 90-91 0.005134597270518913 92-93 0.002813208466223006 94-95 0.004367358597912639 96-97 0.0017115324235063044 98-99 0.0035607743523521965 100-101 0.0033640464875813567 102-103 8.85275391468778E-4 104-105 0.004839505473362654 106-107 8.85275391468778E-4 108-109 0.0015344773452125489 110-111 0.002321388804295907 112-113 0.003993575654848043 114-115 0.002439425523158411 116-117 0.004918196619270989 118-119 0.0035607743523521965 120-121 0.006806784121071049 122-123 0.004268994665527219 124-125 0.05638220604332262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2541582.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.67383967602269 #Duplication Level Percentage of deduplicated Percentage of total 1 80.30771323288424 48.72577317440024 2 10.191350009925706 12.366966731689292 3 3.89291612861994 7.0859450718026755 4 1.8732188796470004 4.5462152792720385 5 1.0077174954032688 3.057104487741053 6 0.6518726211698311 2.3730968943628206 7 0.440943105806808 1.872759791557884 8 0.3084401961455567 1.4971400808461197 9 0.21395774449513077 1.1683474098246893 >10 1.020754647307094 10.638045762835045 >50 0.060277425967527186 2.488409249016605 >100 0.029272240920868692 3.055020520288189 >500 0.001021481548078825 0.4143796105494373 >1k 5.447901589749869E-4 0.7107959358139105 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3460 0.136135682421421 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3119 0.12271884204404973 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.934557295416792E-5 0.0 10-11 0.0 0.0 0.0 3.934557295416792E-5 0.0 12-13 0.0 1.967278647708396E-5 0.0 3.934557295416792E-5 0.0 14-15 0.0 3.934557295416792E-5 0.0 3.934557295416792E-5 0.0 16-17 0.0 7.869114590833584E-5 0.0 9.836393238541979E-5 0.0 18-19 0.0 7.869114590833584E-5 0.0 1.1803671886250375E-4 0.0 20-21 0.0 7.869114590833584E-5 0.0 3.147645836333433E-4 0.0 22-23 0.0 1.1803671886250375E-4 0.0 6.098563807896027E-4 0.0 24-25 0.0 1.1803671886250375E-4 0.0 0.0010229848968083659 0.0 26-27 0.0 1.1803671886250375E-4 0.0 0.001849241928845892 0.0 28-29 0.0 1.1803671886250375E-4 0.0 0.002852554039177174 0.0 30-31 0.0 1.1803671886250375E-4 0.0 0.006393655605052287 0.0 32-33 0.0 1.1803671886250375E-4 0.0 0.012905347928967076 0.0 34-35 0.0 1.1803671886250375E-4 0.0 0.022899123459325727 0.0 36-37 0.0 1.1803671886250375E-4 0.0 0.03775207724952412 0.0 38-39 0.0 1.1803671886250375E-4 0.0 0.0636414642533666 0.0 40-41 0.0 1.1803671886250375E-4 0.0 0.0966327271754364 0.0 42-43 0.0 1.1803671886250375E-4 0.0 0.1282665678305874 0.0 44-45 0.0 1.1803671886250375E-4 0.0 0.1636972562758156 1.967278647708396E-5 46-47 0.0 1.1803671886250375E-4 0.0 0.20894466517310872 3.934557295416792E-5 48-49 0.0 1.1803671886250375E-4 0.0 0.2605463841025 3.934557295416792E-5 50-51 0.0 1.1803671886250375E-4 0.0 0.31948605238784344 3.934557295416792E-5 52-53 0.0 1.1803671886250375E-4 0.0 0.3740583620752744 3.934557295416792E-5 54-55 0.0 1.5738229181667168E-4 0.0 0.43234882840687416 3.934557295416792E-5 56-57 0.0 1.5738229181667168E-4 0.0 0.5015576912332556 3.934557295416792E-5 58-59 0.0 1.5738229181667168E-4 0.0 0.5866818383195977 3.934557295416792E-5 60-61 1.967278647708396E-5 1.5738229181667168E-4 0.0 0.6837080212245759 3.934557295416792E-5 62-63 3.934557295416792E-5 1.5738229181667168E-4 0.0 0.7829375562149874 3.934557295416792E-5 64-65 3.934557295416792E-5 1.9672786477083957E-4 0.0 0.8792751915932675 3.934557295416792E-5 66-67 3.934557295416792E-5 1.9672786477083957E-4 0.0 0.9928068423525189 3.934557295416792E-5 68-69 3.934557295416792E-5 1.9672786477083957E-4 0.0 1.128450705112013 3.934557295416792E-5 70-71 3.934557295416792E-5 1.9672786477083957E-4 0.0 1.2873281287009428 3.934557295416792E-5 72-73 3.934557295416792E-5 1.9672786477083957E-4 0.0 1.4542517219590003 3.934557295416792E-5 74-75 3.934557295416792E-5 1.9672786477083957E-4 0.0 1.6195031283665056 3.934557295416792E-5 76-77 3.934557295416792E-5 1.9672786477083957E-4 0.0 1.7961057325712884 3.934557295416792E-5 78-79 3.934557295416792E-5 1.9672786477083957E-4 0.0 2.0109915792604762 3.934557295416792E-5 80-81 3.934557295416792E-5 1.9672786477083957E-4 0.0 2.2508421919890838 3.934557295416792E-5 82-83 3.934557295416792E-5 1.9672786477083957E-4 0.0 2.5013751277747485 3.934557295416792E-5 84-85 3.934557295416792E-5 1.9672786477083957E-4 0.0 2.763475662008938 3.934557295416792E-5 86-87 3.934557295416792E-5 1.9672786477083957E-4 0.0 3.0347830603144024 3.934557295416792E-5 88-89 3.934557295416792E-5 1.9672786477083957E-4 0.0 3.3359144029191268 3.934557295416792E-5 90-91 3.934557295416792E-5 1.9672786477083957E-4 0.0 3.6793225636631046 3.934557295416792E-5 92-93 3.934557295416792E-5 1.9672786477083957E-4 0.0 4.050115243183183 3.934557295416792E-5 94-95 3.934557295416792E-5 1.9672786477083957E-4 0.0 4.41551757920854 3.934557295416792E-5 96-97 3.934557295416792E-5 1.9672786477083957E-4 0.0 4.782336355860247 3.934557295416792E-5 98-99 3.934557295416792E-5 1.9672786477083957E-4 0.0 5.1822447593664105 3.934557295416792E-5 100-101 3.934557295416792E-5 1.9672786477083957E-4 0.0 5.637630420737949 3.934557295416792E-5 102-103 3.934557295416792E-5 1.9672786477083957E-4 0.0 6.126872947636551 3.934557295416792E-5 104-105 3.934557295416792E-5 2.360734377250075E-4 0.0 6.60816766879841 3.934557295416792E-5 106-107 3.934557295416792E-5 2.360734377250075E-4 0.0 7.085173722508264 3.934557295416792E-5 108-109 3.934557295416792E-5 2.360734377250075E-4 0.0 7.584351006577792 3.934557295416792E-5 110-111 3.934557295416792E-5 2.360734377250075E-4 0.0 8.159878374964883 3.934557295416792E-5 112-113 3.934557295416792E-5 2.360734377250075E-4 0.0 8.773197166174453 3.934557295416792E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13840 0.0 34.407974 1 TACATGG 13895 0.0 32.241802 2 GTATCAA 12860 0.0 31.706976 1 ACATGGG 14095 0.0 29.674967 3 CATGGGA 10050 0.0 26.854303 4 ATCAACG 14705 0.0 26.62324 3 CAACGCA 14820 0.0 26.612858 5 TCAACGC 14755 0.0 26.528206 4 TATCAAC 15365 0.0 26.369228 2 AACGCAG 14735 0.0 26.146488 6 GAGTACT 5050 0.0 23.674732 12-13 ACGCAGA 16315 0.0 23.14035 7 CGCATAG 335 3.45608E-11 23.110487 9 CGCAGAG 15975 0.0 23.001429 8 GGTATCA 9555 0.0 22.676493 1 ATGGGAG 4205 0.0 22.38581 5 CATGGGG 4775 0.0 21.46035 4 AACGCAT 500 0.0 21.436886 6 ACGCATA 425 1.8189894E-12 21.01904 8 GCAGAGT 16645 0.0 20.644415 9 >>END_MODULE