FastQCFastQC Report
Thu 26 May 2016
SRR1364503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541582
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102270.4023871746022753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97180.38236027796860383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62090.24429666247242857No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26510.10430511390149914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116800.036.7171861
GTATCAA164300.034.0161671
GTACATG128350.031.507731
TACATGG131000.029.8386422
ACATGGG128200.027.7480723
TATCAAC205650.027.1781942
ATCAACG202650.026.4043963
TCAACGC202450.026.106724
CAACGCA203850.025.6531285
AACGCAG205250.025.1882936
GAGTACT123650.025.15500612-13
AGTACTT128800.024.05684912-13
CGTATAG2501.9594154E-823.858511
GTACTTT135650.023.5435214-15
CGCGGAA3050.023.39905282-83
CATGGGG43550.022.9862234
CGCAGAG230750.022.6789888
ACGCAGA232200.022.0598777
CATGGGA92000.021.632424
GCAGAGT235250.021.4615359
Produced by FastQC (version 0.11.5)