##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364503_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2541582 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.84929740610376 31.0 28.0 31.0 25.0 34.0 2 29.012753474017362 31.0 28.0 33.0 25.0 34.0 3 29.131924132292408 31.0 28.0 34.0 25.0 34.0 4 32.55617485487385 35.0 32.0 37.0 26.0 37.0 5 31.666797293968873 35.0 32.0 37.0 20.0 37.0 6 31.70594889324838 35.0 32.0 37.0 19.0 37.0 7 31.142576946169747 35.0 31.0 37.0 17.0 37.0 8 31.268603963987783 35.0 32.0 37.0 17.0 37.0 9 31.81799603554007 37.0 30.0 39.0 11.0 39.0 10-11 32.01855261801508 37.0 30.5 39.0 14.0 39.0 12-13 31.75721991263709 37.0 30.5 39.0 11.0 39.0 14-15 32.68210586949388 37.5 31.0 40.0 10.5 41.0 16-17 32.7593390651964 38.0 31.0 40.0 11.0 41.0 18-19 32.651213889616784 38.0 31.0 40.0 10.0 41.0 20-21 32.54608291213897 38.0 30.5 40.0 10.0 41.0 22-23 32.514832887547996 38.0 30.5 40.0 10.0 41.0 24-25 32.31729135632846 37.0 30.0 40.0 10.0 41.0 26-27 32.39554065145252 37.0 30.5 40.0 10.0 41.0 28-29 32.264166766997874 37.0 30.0 40.0 8.5 41.0 30-31 31.717413996479358 37.0 30.0 40.0 8.0 41.0 32-33 31.615978748669136 37.0 30.0 40.0 4.5 41.0 34-35 31.390209523045094 36.0 30.0 40.0 2.0 41.0 36-37 31.346094086281695 36.0 30.0 40.0 2.0 41.0 38-39 31.099635187847568 36.0 30.0 40.0 2.0 41.0 40-41 30.803480863493682 36.0 28.5 40.0 2.0 41.0 42-43 30.6748920554206 36.0 28.5 40.0 2.0 41.0 44-45 30.56562389094666 36.0 28.0 40.0 2.0 41.0 46-47 30.35380286766274 36.0 28.0 40.0 2.0 41.0 48-49 30.022149393566686 36.0 27.0 40.0 2.0 41.0 50-51 29.669081697934594 35.0 26.5 40.0 2.0 41.0 52-53 29.585490060914815 35.0 26.0 40.0 2.0 41.0 54-55 29.13290403378683 35.0 25.0 39.5 2.0 41.0 56-57 29.159490034159827 35.0 26.0 39.0 2.0 41.0 58-59 28.92579346249698 35.0 25.5 39.0 2.0 41.0 60-61 28.65445694846753 34.5 24.5 39.0 2.0 41.0 62-63 28.39553101178715 34.0 24.0 39.0 2.0 41.0 64-65 27.933223873949373 34.0 23.0 38.5 2.0 40.0 66-67 27.558561163873524 34.0 22.0 38.0 2.0 40.0 68-69 27.38780865618343 34.0 22.0 38.0 2.0 40.0 70-71 27.47315077774394 34.0 22.5 37.0 2.0 40.0 72-73 27.239200033679808 34.0 22.0 37.0 2.0 39.5 74-75 26.86707373596445 33.0 21.5 36.0 2.0 39.0 76-77 26.774919912086254 33.0 22.0 36.0 2.0 39.0 78-79 26.366504405523806 33.0 20.0 36.0 2.0 38.5 80-81 26.131686878487493 33.0 20.0 35.0 2.0 37.0 82-83 25.98329839446455 33.0 20.0 35.0 2.0 37.0 84-85 25.53228500988754 33.0 18.0 35.0 2.0 36.5 86-87 25.385922232688145 33.0 18.0 35.0 2.0 36.0 88-89 25.317636220275404 32.5 18.0 35.0 2.0 36.0 90-91 25.132239093603907 32.0 17.5 35.0 2.0 35.5 92-93 25.14178452633045 32.0 18.0 35.0 2.0 35.0 94-95 25.147420976383998 33.0 18.0 35.0 2.0 35.0 96-97 25.014929284201727 33.0 17.5 35.0 2.0 35.0 98-99 24.86763382019545 32.0 15.0 35.0 2.0 35.0 100-101 24.762435561787896 32.0 11.0 35.0 2.0 35.0 102-103 23.641704654817353 30.0 10.0 34.0 2.0 35.0 104-105 24.56302432894158 32.0 10.5 35.0 2.0 35.0 106-107 24.658482197308608 32.0 9.0 35.0 2.0 35.0 108-109 24.723557414240425 33.0 9.0 35.0 2.0 35.0 110-111 24.65169252851177 33.0 7.0 35.0 2.0 35.0 112-113 24.590850501774092 33.0 4.5 35.0 2.0 35.0 114-115 24.52742740545062 33.0 2.0 35.0 2.0 35.0 116-117 24.443152139100768 33.0 2.0 35.0 2.0 35.0 118-119 24.322757046595388 33.0 2.0 35.0 2.0 35.0 120-121 24.15761482415283 33.0 2.0 35.0 2.0 35.0 122-123 23.8647789054219 32.0 2.0 35.0 2.0 35.0 124-125 23.0979161797652 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0785491223239312 1101 2 0.9878860493768045 1101 3 1.0090376394814093 1101 4 1.1091241697193084 1101 5 1.3853849704802776 1101 6 1.320597264813827 1101 7 1.7099914567861134 1101 8 1.5617117679887613 1101 9 2.375462719956108 1101 10-11 2.0274069401769665 1101 12-13 2.1263676334680603 1101 14-15 1.987101881250382 1101 16-17 1.931940106795615 1101 18-19 2.126350836074188 1101 20-21 2.304327482080872 1101 22-23 2.1779390467497564 1101 24-25 2.384277520696834 1101 26-27 2.0827075530644095 1101 28-29 2.13471118748231 1101 30-31 2.6094958795771817 1101 32-33 2.39430573107035 1101 34-35 2.478054719337319 1101 36-37 2.417362033339465 1101 38-39 2.44747912524398 1101 40-41 2.5238905542951926 1101 42-43 2.4391223794522645 1101 44-45 2.3942329767806534 1101 46-47 2.265536686633272 1101 48-49 2.337388277022562 1101 50-51 2.4876327578117206 1101 52-53 2.535102301892575 1101 54-55 2.8446782869268255 1101 56-57 2.5216386783125984 1101 58-59 2.6311748638172574 1101 60-61 2.6298793632442035 1101 62-63 2.6225634737241172 1101 64-65 2.814539570040157 1101 66-67 2.951821409482406 1101 68-69 2.9670404168058404 1101 70-71 2.602134671720325 1101 72-73 2.672940874202993 1101 74-75 2.6973935254800203 1101 76-77 2.456254212305911 1101 78-79 2.6696896879016023 1101 80-81 2.7835731626340916 1101 82-83 2.8064021906252385 1101 84-85 2.9774121267102167 1101 86-87 2.676498485526743 1101 88-89 2.6484960293414552 1101 90-91 2.721453847172903 1101 92-93 2.5467778634171268 1101 94-95 2.5388113854535206 1101 96-97 2.4076212251288815 1101 98-99 2.5005194062535274 1101 100-101 2.5293530315260675 1101 102-103 2.2869715832616997 1101 104-105 2.429676754702676 1101 106-107 2.4865496379448544 1101 108-109 2.447972599758568 1101 110-111 2.3815121943034256 1101 112-113 2.297298816365874 1101 114-115 2.3794295555003693 1101 116-117 2.371739368632884 1101 118-119 2.2895277101314164 1101 120-121 2.266838555849038 1101 122-123 2.3204394483311894 1101 124-125 2.2606619748345054 1102 1 0.415783838679733 1102 2 -1.8704708418567364 1102 3 -0.12490512312001911 1102 4 0.394119967140405 1102 5 0.962514575889319 1102 6 0.4738154576650615 1102 7 1.217262781388527 1102 8 0.9177955486898242 1102 9 1.6848358651449225 1102 10-11 1.2643267618760845 1102 12-13 1.5177423215942092 1102 14-15 1.5435705331985048 1102 16-17 1.4805365251095637 1102 18-19 1.5300189637954063 1102 20-21 1.7134733693295914 1102 22-23 1.6573401692964254 1102 24-25 1.9222082850350617 1102 26-27 1.6796463759970806 1102 28-29 1.6909331951863678 1102 30-31 2.0177361874210717 1102 32-33 1.5768969628840743 1102 34-35 1.9058870782654473 1102 36-37 1.3529149214340137 1102 38-39 1.2371222579113592 1102 40-41 1.4560862922295428 1102 42-43 1.2522013627070123 1102 44-45 1.2430142952883294 1102 46-47 1.071544581904746 1102 48-49 1.1490636785912614 1102 50-51 1.3520774132334417 1102 52-53 1.1867525278226871 1102 54-55 1.387170276798141 1102 56-57 1.128879130889846 1102 58-59 1.134052667694256 1102 60-61 1.198736460955157 1102 62-63 1.4639139953617537 1102 64-65 1.474708283997895 1102 66-67 1.6108988471441492 1102 68-69 1.5495837803826902 1102 70-71 1.018175842387521 1102 72-73 1.424198062737453 1102 74-75 1.5923644393849123 1102 76-77 1.4922348605254925 1102 78-79 1.2139253828674015 1102 80-81 1.3466858727464377 1102 82-83 0.9622251193115225 1102 84-85 0.9979119481172134 1102 86-87 1.1235603318199097 1102 88-89 1.3216825800266818 1102 90-91 1.0430341894606734 1102 92-93 1.0996046122675587 1102 94-95 1.169067895467343 1102 96-97 1.2735895643732675 1102 98-99 1.013351944438572 1102 100-101 0.8769456110862635 1102 102-103 0.6960648220890135 1102 104-105 1.0520804173862963 1102 106-107 1.2991560468444945 1102 108-109 1.080570802674746 1102 110-111 0.9629955519581586 1102 112-113 1.0705904647195652 1102 114-115 1.2483790701124633 1102 116-117 0.9653347180444563 1102 118-119 0.5685304175748307 1102 120-121 0.8398047533051844 1102 122-123 1.204960546870712 1102 124-125 1.3049701692658537 1103 1 0.7753298866463822 1103 2 0.7153308662193787 1103 3 0.5193043467733283 1103 4 0.6867055290376669 1103 5 1.0463136359471115 1103 6 1.1282064246722996 1103 7 1.3099910152073022 1103 8 1.2077577136329296 1103 9 1.9028152468277604 1103 10-11 1.6917305896870403 1103 12-13 1.7514015877242706 1103 14-15 1.566954595793021 1103 16-17 1.539064192333214 1103 18-19 1.733638179605471 1103 20-21 1.869923257054836 1103 22-23 1.8656617365198258 1103 24-25 2.1138811532502686 1103 26-27 1.5488783582707484 1103 28-29 1.6920742933205446 1103 30-31 2.241945897587293 1103 32-33 2.0723100282629794 1103 34-35 2.096948141768646 1103 36-37 2.0124500053004724 1103 38-39 2.074241671286458 1103 40-41 2.256759346722248 1103 42-43 2.2534652376404907 1103 44-45 2.0120828854053805 1103 46-47 1.9009955280188002 1103 48-49 1.9363783862880837 1103 50-51 1.9714059667207557 1103 52-53 1.9518076697876516 1103 54-55 2.2881433621434653 1103 56-57 2.1271137193321117 1103 58-59 2.1332351128298583 1103 60-61 2.1916670256584645 1103 62-63 2.2593818416362232 1103 64-65 2.342950592856532 1103 66-67 2.4660737440149383 1103 68-69 2.5791735830962175 1103 70-71 2.1865550143728676 1103 72-73 2.2528396391070658 1103 74-75 2.359894590000362 1103 76-77 2.3205035188694723 1103 78-79 2.342971488988013 1103 80-81 2.2769723479842696 1103 82-83 2.182540659646275 1103 84-85 2.365484925392547 1103 86-87 2.25584969270858 1103 88-89 2.0968771383477076 1103 90-91 2.2165699540122645 1103 92-93 2.083887168681187 1103 94-95 1.9953035086338247 1103 96-97 1.8197463799180262 1103 98-99 2.038707267756749 1103 100-101 1.9595567065972403 1103 102-103 1.8634459045077598 1103 104-105 1.9372209393936437 1103 106-107 1.9498694526583833 1103 108-109 1.9193261838960503 1103 110-111 1.9509278467581908 1103 112-113 1.843265656320682 1103 114-115 1.6711057949647703 1103 116-117 1.7977884810279399 1103 118-119 1.7965217191686413 1103 120-121 1.7639326518772869 1103 122-123 1.8622326430970908 1103 124-125 1.690238418749896 1104 1 0.6728639450711356 1104 2 0.6369017833958317 1104 3 0.6246169283721876 1104 4 0.7043804859931981 1104 5 0.9839670480101894 1104 6 1.0269965235078757 1104 7 1.4613019173673933 1104 8 1.1688482114935255 1104 9 1.778310179022558 1104 10-11 1.2717618503922807 1104 12-13 1.3030363523799728 1104 14-15 1.3672351065182227 1104 16-17 1.242369654631453 1104 18-19 1.2093032968328075 1104 20-21 1.450591105263669 1104 22-23 1.4766975216276421 1104 24-25 1.820182841839916 1104 26-27 1.6721147830453376 1104 28-29 1.5597748466314272 1104 30-31 1.9789249391201906 1104 32-33 1.9139793116805102 1104 34-35 1.8112276611705305 1104 36-37 1.7072288330482408 1104 38-39 1.9600399901342271 1104 40-41 2.084027063987996 1104 42-43 2.000232789830548 1104 44-45 1.73657071307505 1104 46-47 1.7487368529775402 1104 48-49 1.4123987359803607 1104 50-51 1.9659012913971452 1104 52-53 1.7903769260257327 1104 54-55 2.027401087704593 1104 56-57 2.0736515437673155 1104 58-59 2.1181227713388395 1104 60-61 2.155762192944895 1104 62-63 2.2118987710519953 1104 64-65 2.333363196419956 1104 66-67 2.274585658953839 1104 68-69 2.4362300754878987 1104 70-71 2.1437730003351625 1104 72-73 2.3754563316059993 1104 74-75 2.4086303175334614 1104 76-77 2.2216789583873933 1104 78-79 2.233931791232358 1104 80-81 2.112587935053412 1104 82-83 2.1167580574449296 1104 84-85 2.4175436161032735 1104 86-87 2.285343516866149 1104 88-89 2.152275740586802 1104 90-91 2.052519262178105 1104 92-93 1.8102272089673548 1104 94-95 1.8108789746731162 1104 96-97 1.7970061010388108 1104 98-99 1.9425069079550603 1104 100-101 1.9604486057946566 1104 102-103 1.868023573335993 1104 104-105 1.7825839078805146 1104 106-107 1.8644710590143774 1104 108-109 1.73589322157639 1104 110-111 1.8578363608025406 1104 112-113 1.88291259796285 1104 114-115 1.7808430446211787 1104 116-117 1.7766746230009822 1104 118-119 1.7344020715105763 1104 120-121 1.831677537852137 1104 122-123 1.9721050139453844 1104 124-125 1.7348716210532338 1105 1 0.5770663838811352 1105 2 0.726972206306467 1105 3 0.5638214865766855 1105 4 0.7463289998941107 1105 5 0.8193908271081618 1105 6 1.0403937126663507 1105 7 1.3680380213239758 1105 8 1.1917734395779433 1105 9 1.7254506577466024 1105 10-11 1.4050740269738569 1105 12-13 1.4819517121073638 1105 14-15 1.473040809307335 1105 16-17 1.4348174319203437 1105 18-19 1.6100158014339172 1105 20-21 1.7648969065528632 1105 22-23 1.6995501485176447 1105 24-25 1.8984268388827985 1105 26-27 1.7641353092477914 1105 28-29 1.7736423938355301 1105 30-31 2.0414496803699507 1105 32-33 1.8210902924499912 1105 34-35 2.0882290198393996 1105 36-37 1.787121885141211 1105 38-39 1.9402134234280979 1105 40-41 1.9957531673036186 1105 42-43 1.9490157218054804 1105 44-45 1.8954470397422334 1105 46-47 1.7699641788967746 1105 48-49 1.8951827004394985 1105 50-51 1.9597125850733157 1105 52-53 1.8611156692142536 1105 54-55 2.141490808697064 1105 56-57 1.8636393233101174 1105 58-59 1.8902458343306705 1105 60-61 1.8566135026984618 1105 62-63 1.873225666980236 1105 64-65 2.164533712152508 1105 66-67 2.292693340311459 1105 68-69 2.2804903472985387 1105 70-71 2.2312552390158054 1105 72-73 2.3285617063631747 1105 74-75 2.3030029253110875 1105 76-77 2.1468994491818805 1105 78-79 2.2465894858989586 1105 80-81 2.200543116709511 1105 82-83 2.0854633287057247 1105 84-85 2.341967024880603 1105 86-87 2.232590027303875 1105 88-89 2.14558972971869 1105 90-91 2.193489897747259 1105 92-93 1.9464554591384768 1105 94-95 1.8981340246775602 1105 96-97 1.9868405576698258 1105 98-99 2.0235636433833406 1105 100-101 2.0600976194362666 1105 102-103 1.8767711742173745 1105 104-105 1.4387833134582344 1105 106-107 1.640344005932313 1105 108-109 1.69712544212533 1105 110-111 1.7425331485344273 1105 112-113 1.796541259218401 1105 114-115 1.6534951751798452 1105 116-117 1.7466016610321837 1105 118-119 1.8136629315942123 1105 120-121 1.87877963935669 1105 122-123 1.8842219871648922 1105 124-125 1.7905244782437322 1106 1 0.3351794163048538 1106 2 0.21084045940861884 1106 3 0.23230740381464088 1106 4 0.15031335877100105 1106 5 0.4188987576126273 1106 6 0.6244286383379318 1106 7 0.885175763219685 1106 8 0.5088380629668094 1106 9 1.2367793861670329 1106 10-11 0.8337766646378171 1106 12-13 0.7516945138769806 1106 14-15 0.9376505239677542 1106 16-17 0.8170347989356657 1106 18-19 0.9613882633325304 1106 20-21 0.7883366215687104 1106 22-23 0.7724179299669203 1106 24-25 0.5434131612018334 1106 26-27 0.5711790608446137 1106 28-29 0.578588360193315 1106 30-31 0.9158539984401273 1106 32-33 0.6875623699492941 1106 34-35 0.7252730006023249 1106 36-37 0.7470199321094029 1106 38-39 0.9474355963028032 1106 40-41 1.1923580494001769 1106 42-43 1.1659366523663266 1106 44-45 1.0912992406111819 1106 46-47 1.0869258353674418 1106 48-49 1.0681939853233295 1106 50-51 1.2015405565827315 1106 52-53 0.9631547172241746 1106 54-55 1.497973958770114 1106 56-57 1.1944490517457602 1106 58-59 1.1270856552427269 1106 60-61 1.377989049816751 1106 62-63 1.514478759854569 1106 64-65 1.4653797517361262 1106 66-67 1.5123223134471928 1106 68-69 1.311433800152411 1106 70-71 1.1984123590203701 1106 72-73 1.1834968173183924 1106 74-75 1.4347204987337285 1106 76-77 1.0619310030814617 1106 78-79 0.9545515685680748 1106 80-81 1.1422629256064454 1106 82-83 0.8638834134037516 1106 84-85 1.1237336291168702 1106 86-87 1.2629814297492885 1106 88-89 0.9929196826310545 1106 90-91 1.0614537645028896 1106 92-93 1.007722101221873 1106 94-95 0.9176961924222198 1106 96-97 0.8453835195905626 1106 98-99 0.8342092169201223 1106 100-101 0.674526851586517 1106 102-103 0.5828261908748438 1106 104-105 0.7748068232488343 1106 106-107 0.7860367659059477 1106 108-109 0.6820369691739714 1106 110-111 0.6546580808025837 1106 112-113 0.5053555178196554 1106 114-115 0.5454891911036732 1106 116-117 0.8753898856066264 1106 118-119 0.6204076624626147 1106 120-121 0.6610234835795481 1106 122-123 0.881464639155837 1106 124-125 0.9257258183414443 1107 1 0.253101534401047 1107 2 0.3212487281928418 1107 3 0.11724921358717921 1107 4 0.2334704703010786 1107 5 0.2725434853514628 1107 6 0.44042088481371167 1107 7 0.6850956321833799 1107 8 0.6464835738044101 1107 9 1.194142633890248 1107 10-11 0.5916551210662746 1107 12-13 0.8481200258393322 1107 14-15 0.6902900034956403 1107 16-17 0.7455850844634071 1107 18-19 0.7165138826471349 1107 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-3.8894429569470503 2216 112-113 -3.987220887557875 2216 114-115 -3.8808717756909985 2216 116-117 -3.6866282527811123 2216 118-119 -3.9211559838991263 2216 120-121 -3.8470358642929803 2216 122-123 -3.8111542860532133 2216 124-125 -3.8161895365305014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 134118.0 3 43916.0 4 20453.0 5 17026.0 6 17884.0 7 21073.0 8 23387.0 9 24156.0 10 26049.0 11 28256.0 12 31672.0 13 35175.0 14 37710.0 15 30220.0 16 20803.0 17 17797.0 18 15493.0 19 14506.0 20 14181.0 21 14928.0 22 17685.0 23 23945.0 24 32479.0 25 43146.0 26 52600.0 27 59384.0 28 66542.0 29 75985.0 30 84194.0 31 95565.0 32 111597.0 33 132662.0 34 160865.0 35 202359.0 36 276918.0 37 366780.0 38 149185.0 39 888.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.41677718088502 20.725600843518563 14.587286013685514 23.2703359619109 2 15.234327047469758 21.55178519155044 39.20091313421279 24.012974626767015 3 18.449729341803646 26.588282416227372 30.488372989736312 24.473615252232666 4 12.28261525101905 18.08496842035556 37.56355642415071 32.06885990447468 5 12.307255874490771 39.53694982101699 34.4011328377365 13.754661466755744 6 29.898500105642988 38.64526737118933 17.519345257416745 13.936887265750936 7 26.316456538227946 33.894856665127655 21.559528125170974 18.229158671473424 8 25.38407181039211 36.29979280621282 19.755372834714755 18.56076254868031 9 25.649064244238428 16.233707981879004 20.797676407843618 37.31955136603895 10-11 24.100511868547418 27.815419639156186 27.444172805751403 20.639895686544993 12-13 25.422627133398713 25.188716827869364 28.18070308834393 21.207952950387998 14-15 22.296718942025482 26.171385560952693 26.981100487552297 24.550795009469528 16-17 20.990271630615887 29.734255707638585 27.53176342409328 21.743709237652244 18-19 20.74563656419428 27.641103556436995 30.850153636771942 20.76310624259678 20-21 21.76747716268619 26.708536796734382 30.88664433479395 20.637341705785477 22-23 21.222924703522047 26.67590382469886 30.676617563361518 21.424553908417572 24-25 21.08144318336078 26.702295723136064 30.914295494921234 21.30196559858192 26-27 21.166215551035876 26.96434002747222 30.833291035094945 21.036153386396965 28-29 20.749583711062424 26.902908671400873 31.234536715267808 21.112970902268895 30-31 20.774480113221667 26.78066607044563 31.177743774205087 21.26711004212762 32-33 20.823696121370148 26.826889803411696 30.848442534084597 21.50097154113356 34-35 21.170739960378587 26.72358064255938 30.814029206529074 21.291650190532962 36-37 21.34807521531093 26.604734814131653 30.66830686858489 21.37888310197253 38-39 21.22209058885233 26.76045642635992 30.817632086610995 21.199820898176757 40-41 21.1582200050245 26.549760155873596 30.92530473080072 21.366715108301186 42-43 21.194423897134044 26.83950014951447 30.43156565259132 21.53451030076016 44-45 21.291973712218592 27.036671557361796 29.995994587578263 21.67536014284135 46-47 21.413518918750903 27.174288109871853 29.308296141886597 22.103896829490644 48-49 21.683334113687504 27.289116498746253 28.992446040476384 22.035103347089862 50-51 21.75446358370286 27.384505958694078 28.66467115247341 22.196359305129654 52-53 21.726968371261826 27.657804335175623 28.629837097460204 21.985390196102347 54-55 21.441449605408835 27.408188111439006 28.787641790809555 22.362720492342607 56-57 21.679577301310132 27.80249561885666 28.390654767478736 22.12727231235447 58-59 21.605775457962796 27.3241429944027 28.77032887390609 22.29975267372841 60-61 21.754127940786486 27.4284481083042 28.78040134058236 22.037022610326954 62-63 21.889984580403222 27.327999099769396 28.748734542532944 22.033281777294437 64-65 21.696334274625755 27.10548151093674 28.763094512780285 22.43508970165722 66-67 21.553973459096223 27.51508721651189 28.558398255276096 22.37254106911579 68-69 21.605776806801384 27.41785746254489 28.69605761068328 22.280308119970453 70-71 21.414618858764946 27.54851086073149 28.74488224264352 22.291988037860044 72-73 21.6782111313116 27.345823075870605 28.383791355087524 22.592174437730268 74-75 21.55418810117956 27.4629524265954 28.339986983869913 22.642872488355128 76-77 21.669105470014916 27.372246116243375 28.54460295245376 22.414045461287948 78-79 21.601757670677152 27.406985237919212 28.511982177773326 22.47927491363031 80-81 21.268305542221768 27.82790626303323 28.39349931932105 22.51028887542395 82-83 21.598152486732165 27.631804408166026 28.195902664426853 22.57414044067496 84-85 21.436987167242126 27.294737548254894 28.117689714061893 23.15058557044108 86-87 21.55525185067294 27.27495209172421 28.368033922265933 22.80176213533692 88-89 21.735635474056654 27.058219450944748 28.361578919929688 22.84456615506891 90-91 21.476675767983888 27.344123630498977 28.58066075995835 22.598539841558782 92-93 21.431369243145205 27.3854334278501 28.46185429257125 22.721343036433446 94-95 21.27918762551702 27.321828786915383 28.33401141378594 23.06497217378166 96-97 21.52664123700753 27.18384526113403 28.17376448468545 23.115749017172988 98-99 21.29183713136149 27.017483598797913 28.15421654701678 23.536462722823817 100-101 21.365047276212614 27.176021904717313 28.362973347398224 23.09595747167185 102-103 21.6008426357312 26.97022563798691 28.358729263138343 23.07020246314354 104-105 21.44466381856283 26.85790380291898 28.473870916211762 23.22356146230643 106-107 21.188028005397427 27.08600924267036 28.34650393859099 23.37945881334122 108-109 21.400234184850223 27.06269166212225 27.93620272727774 23.60087142574979 110-111 21.095493504817483 26.960417473252818 28.247748641000705 23.696340380928998 112-113 21.459727562847792 26.895939017389598 28.097751907575493 23.546581512187117 114-115 21.155384795466595 27.069920157854714 28.040419778661086 23.734275268017598 116-117 21.045828936465554 26.940700713177858 28.208395400974666 23.805074949381922 118-119 21.055874520765265 27.093150169660113 27.93004038477277 23.920934924801855 120-121 20.806252310375132 27.135347308278952 28.004707410847306 24.05369297049861 122-123 21.05419091931906 27.09801714340461 27.672723116368857 24.17506882090747 124-125 20.987610843025756 27.006870885471272 27.782572862609246 24.222945408893725 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 853.0 1 647.5 2 655.0 3 932.0 4 744.0 5 594.5 6 1161.5 7 2687.5 8 4297.0 9 5030.5 10 4102.0 11 2619.5 12 2200.5 13 2188.5 14 2359.5 15 2607.0 16 2958.5 17 3348.0 18 3772.5 19 4297.5 20 4925.0 21 5958.0 22 7262.5 23 8716.0 24 10573.5 25 13005.0 26 16781.0 27 21391.5 28 25546.0 29 29867.5 30 35139.0 31 41273.5 32 48072.5 33 54535.5 34 61295.0 35 67312.5 36 72786.5 37 79125.5 38 82056.5 39 82201.5 40 82511.5 41 84051.5 42 85404.5 43 86732.0 44 89211.0 45 90811.0 46 89233.5 47 87981.5 48 95776.0 49 97376.5 50 90266.5 51 85133.5 52 79298.5 53 75171.5 54 70693.0 55 64502.5 56 58160.5 57 51483.5 58 46280.0 59 41926.5 60 36375.5 61 30350.0 62 24772.0 63 19414.0 64 14574.0 65 11462.5 66 8816.0 67 6821.5 68 5174.5 69 3561.5 70 2726.0 71 2246.0 72 1825.0 73 1582.0 74 1327.5 75 1086.5 76 763.0 77 435.5 78 260.5 79 169.5 80 121.0 81 79.0 82 58.5 83 42.0 84 27.0 85 19.5 86 13.0 87 7.5 88 5.0 89 4.0 90 2.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10595762796557419 2 2.3607343772500747E-4 3 0.0 4 0.183468406685285 5 0.0 6 1.1803671886250374E-4 7 0.03907015394348874 8 0.0 9 0.0 10-11 4.5247408897293104E-4 12-13 3.737829430645952E-4 14-15 3.344373701104273E-4 16-17 0.003836193363031372 18-19 0.001396767839872961 20-21 0.002852554039177174 22-23 0.001396767839872961 24-25 0.004603432035637647 26-27 0.004131285160187631 28-29 0.0030689546904250978 30-31 7.475658861291904E-4 32-33 5.901835943125187E-5 34-35 0.0010623304697625338 36-37 9.83639323854198E-4 38-39 4.328013024958471E-4 40-41 0.001396767839872961 42-43 8.656026049916941E-4 44-45 8.262570320375263E-4 46-47 1.1803671886250374E-4 48-49 3.934557295416791E-5 50-51 0.041332524388353394 52-53 3.934557295416791E-5 54-55 0.13818165221503773 56-57 3.147645836333433E-4 58-59 0.0 60-61 0.0 62-63 4.328013024958471E-4 64-65 0.06431033899358746 66-67 0.053195214634035023 68-69 0.0015541501316896325 70-71 0.0024984438825896627 72-73 0.06942526347762928 74-75 0.00472146875450015 76-77 0.0013770950533958772 78-79 0.001436113412827129 80-81 7.869114590833582E-5 82-83 1.377095053395877E-4 84-85 1.9672786477083956E-5 86-87 2.5574622420209146E-4 88-89 0.0013574222669187931 90-91 3.344373701104273E-4 92-93 0.002262370444864655 94-95 0.003698483857691784 96-97 0.002419752736681327 98-99 0.0 100-101 0.0011410216156708694 102-103 0.0012984039074875411 104-105 3.147645836333433E-4 106-107 1.9672786477083956E-5 108-109 0.0 110-111 2.3607343772500747E-4 112-113 1.9672786477083957E-4 114-115 1.770550782937556E-4 116-117 0.0 118-119 0.0011803671886250376 120-121 0.002262370444864655 122-123 8.459298185146102E-4 124-125 0.008124860815035675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2541582.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.91743623047076 #Duplication Level Percentage of deduplicated Percentage of total 1 77.21451003448738 41.632084208515174 2 11.107925006517625 11.978216763835311 3 4.588198632993875 7.421517216215411 4 2.265464730724419 4.885922006048578 5 1.3041435185466557 3.5158037498310533 6 0.8281170667940019 2.6789969484138054 7 0.5299329834813957 2.000083949029689 8 0.3930815974007875 1.6955161568982833 9 0.28596720835940587 1.387675684865161 >10 1.3480382953270948 12.674091074727 >50 0.08463137200511198 3.1509682677236666 >100 0.04691724672491906 4.487532466697127 >500 0.0021730949386386165 0.81391154246995 >1k 6.744087740592273E-4 0.6209888512161226 >5k 1.4986861645760607E-4 0.6434931316408058 >10k+ 7.493430822880303E-5 0.41319798187295287 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10227 0.4023871746022753 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9718 0.38236027796860383 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6209 0.24429666247242857 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2651 0.10430511390149914 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.934557295416792E-5 0.0 2 0.0 0.0 0.0 3.934557295416792E-5 0.0 3 0.0 0.0 0.0 3.934557295416792E-5 0.0 4 0.0 0.0 0.0 3.934557295416792E-5 0.0 5 0.0 0.0 0.0 7.869114590833584E-5 0.0 6 0.0 0.0 0.0 7.869114590833584E-5 0.0 7 0.0 0.0 0.0 7.869114590833584E-5 0.0 8 0.0 0.0 0.0 7.869114590833584E-5 0.0 9 0.0 0.0 0.0 7.869114590833584E-5 0.0 10-11 0.0 0.0 0.0 9.836393238541979E-5 0.0 12-13 0.0 0.0 0.0 1.377095053395877E-4 0.0 14-15 0.0 0.0 0.0 1.5738229181667168E-4 0.0 16-17 0.0 0.0 0.0 2.5574622420209146E-4 0.0 18-19 0.0 1.967278647708396E-5 0.0 2.754190106791754E-4 0.0 20-21 0.0 3.934557295416792E-5 0.0 4.131285160187631E-4 0.0 22-23 0.0 3.934557295416792E-5 0.0 7.278930996521065E-4 0.0 24-25 0.0 3.934557295416792E-5 0.0 0.0012787311210104573 0.0 26-27 0.0 3.934557295416792E-5 0.0 0.0020853153665708994 0.0 28-29 0.0 3.934557295416792E-5 0.0 0.0030296091174709296 0.0 30-31 0.0 3.934557295416792E-5 0.0 0.00651169232391479 0.0 32-33 0.0 3.934557295416792E-5 0.0 0.013239785299077503 0.0 34-35 0.0 3.934557295416792E-5 0.0 0.023272906402390322 0.0 36-37 0.0 3.934557295416792E-5 0.0 0.03879473493280956 0.0 38-39 0.0 3.934557295416792E-5 0.0 0.06551037896868958 0.0 40-41 0.0 3.934557295416792E-5 0.0 0.09802949501530936 0.0 42-43 0.0 3.934557295416792E-5 0.0 0.1298797363217083 0.0 44-45 0.0 3.934557295416792E-5 0.0 0.1654481342722761 0.0 46-47 0.0 3.934557295416792E-5 0.0 0.21108899889911087 0.0 48-49 0.0 3.934557295416792E-5 0.0 0.26339893814167714 0.0 50-51 3.934557295416792E-5 3.934557295416792E-5 0.0 0.32281075330247067 0.0 52-53 3.934557295416792E-5 3.934557295416792E-5 0.0 0.3793109960646558 0.0 54-55 3.934557295416792E-5 3.934557295416792E-5 0.0 0.4379162269798889 0.0 56-57 3.934557295416792E-5 3.934557295416792E-5 0.0 0.5079710196247849 0.0 58-59 3.934557295416792E-5 3.934557295416792E-5 0.0 0.5922492368926126 0.0 60-61 3.934557295416792E-5 3.934557295416792E-5 0.0 0.6884294899790759 0.0 62-63 3.934557295416792E-5 3.934557295416792E-5 0.0 0.7839998866847498 0.0 64-65 3.934557295416792E-5 3.934557295416792E-5 0.0 0.8773669313049903 0.0 66-67 3.934557295416792E-5 3.934557295416792E-5 0.0 0.9882821014627896 0.0 68-69 3.934557295416792E-5 3.934557295416792E-5 0.0 1.1199717341403899 0.0 70-71 3.934557295416792E-5 3.934557295416792E-5 0.0 1.2755834751741237 0.0 72-73 3.934557295416792E-5 3.934557295416792E-5 0.0 1.4370183610050749 0.0 74-75 3.934557295416792E-5 3.934557295416792E-5 0.0 1.596426949828886 0.0 76-77 3.934557295416792E-5 5.9018359431251875E-5 0.0 1.7670686997311122 0.0 78-79 3.934557295416792E-5 7.869114590833584E-5 0.0 1.9710754954984728 0.0 80-81 3.934557295416792E-5 7.869114590833584E-5 0.0 2.1975682862091404 0.0 82-83 3.934557295416792E-5 7.869114590833584E-5 0.0 2.4386386116993277 0.0 84-85 3.934557295416792E-5 7.869114590833584E-5 0.0 2.6926929762643894 0.0 86-87 3.934557295416792E-5 7.869114590833584E-5 0.0 2.95579682260891 0.0 88-89 3.934557295416792E-5 7.869114590833584E-5 0.0 3.2477213011423594 0.0 90-91 3.934557295416792E-5 7.869114590833584E-5 0.0 3.5845194056300365 0.0 92-93 3.934557295416792E-5 7.869114590833584E-5 0.0 3.951141454416973 0.0 94-95 3.934557295416792E-5 7.869114590833584E-5 0.0 4.312963343303501 0.0 96-97 3.934557295416792E-5 7.869114590833584E-5 0.0 4.675277051851957 0.0 98-99 3.934557295416792E-5 7.869114590833584E-5 0.0 5.076916660568103 0.0 100-101 3.934557295416792E-5 7.869114590833584E-5 0.0 5.537436919210161 0.0 102-103 3.934557295416792E-5 7.869114590833584E-5 0.0 6.034804306923798 0.0 104-105 3.934557295416792E-5 7.869114590833584E-5 0.0 6.5236140325199035 0.0 106-107 3.934557295416792E-5 7.869114590833584E-5 0.0 7.010436806681822 0.0 108-109 3.934557295416792E-5 7.869114590833584E-5 0.0 7.518427499093084 0.0 110-111 3.934557295416792E-5 7.869114590833584E-5 0.0 8.106151995095967 0.0 112-113 3.934557295416792E-5 7.869114590833584E-5 0.0 8.728933396601015 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11680 0.0 36.717186 1 GTATCAA 16430 0.0 34.016167 1 GTACATG 12835 0.0 31.50773 1 TACATGG 13100 0.0 29.838642 2 ACATGGG 12820 0.0 27.748072 3 TATCAAC 20565 0.0 27.178194 2 ATCAACG 20265 0.0 26.404396 3 TCAACGC 20245 0.0 26.10672 4 CAACGCA 20385 0.0 25.653128 5 AACGCAG 20525 0.0 25.188293 6 GAGTACT 12365 0.0 25.155006 12-13 AGTACTT 12880 0.0 24.056849 12-13 CGTATAG 250 1.9594154E-8 23.85851 1 GTACTTT 13565 0.0 23.54352 14-15 CGCGGAA 305 0.0 23.399052 82-83 CATGGGG 4355 0.0 22.986223 4 CGCAGAG 23075 0.0 22.678988 8 ACGCAGA 23220 0.0 22.059877 7 CATGGGA 9200 0.0 21.63242 4 GCAGAGT 23525 0.0 21.461535 9 >>END_MODULE