FastQCFastQC Report
Thu 26 May 2016
SRR1364502_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364502_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304887
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61550.050.2897261
GGTATCA34750.048.305691
ATCAACG72750.039.9135063
TCAACGC74550.039.1319434
CAACGCA76700.038.111495
AACGCAG75700.037.4188236
TATCAAC84150.036.9104582
GTACATG109100.034.1549451
TACATGG120500.030.812762
ACGCAGA92300.030.561097
CGCAGAG93250.029.8043738
ACATGGG115600.029.6996293
CATGGGG69600.023.8659064
GCAGAGT109700.023.2735489
AGAGTAC95250.023.08143410-11
CAGAGTA101850.020.6510310-11
CATGGGA64250.020.571354
ACGCATA3953.255991E-1019.5865618
GGACCGT6100.019.5109866
GAGTACA84550.019.4311941
Produced by FastQC (version 0.11.5)