##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364502_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3304887 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.541719883312197 31.0 26.0 31.0 10.0 34.0 2 26.76273288617735 31.0 26.0 31.0 10.0 34.0 3 26.86892713729698 31.0 26.0 31.0 10.0 34.0 4 29.710205220329772 35.0 28.0 37.0 10.0 37.0 5 30.269309964304377 35.0 30.0 37.0 10.0 37.0 6 30.357314183510663 35.0 30.0 37.0 10.0 37.0 7 30.215408575240243 35.0 30.0 37.0 10.0 37.0 8 29.9135008246878 35.0 30.0 37.0 8.0 37.0 9 30.581959988344533 35.0 29.0 39.0 8.0 39.0 10-11 30.697800560200697 35.0 29.0 39.0 7.5 39.0 12-13 30.531384129018633 35.0 28.5 39.0 4.5 39.0 14-15 31.221939509580814 36.5 28.5 40.0 2.0 41.0 16-17 31.471323376563255 37.0 30.0 40.0 2.0 41.0 18-19 31.335992577053318 37.0 29.0 40.0 2.0 41.0 20-21 31.06740623809528 37.0 28.0 40.0 2.0 41.0 22-23 31.128577013374436 37.0 28.5 40.0 2.0 41.0 24-25 31.151174003831294 37.0 29.0 40.0 2.0 41.0 26-27 31.242749449527324 37.0 29.5 40.0 2.0 41.0 28-29 31.195117261195314 37.0 29.5 40.0 2.0 41.0 30-31 30.82592914674541 36.5 28.0 40.0 2.0 41.0 32-33 30.87218156021673 36.5 29.0 40.0 2.0 41.0 34-35 30.577320495375485 36.0 28.0 40.0 2.0 41.0 36-37 30.348946726469013 36.0 27.0 40.0 2.0 41.0 38-39 30.207259582551536 36.0 27.0 40.0 2.0 41.0 40-41 30.26038030952344 36.0 27.5 40.0 2.0 41.0 42-43 30.14722197763494 36.0 27.0 40.0 2.0 41.0 44-45 29.79373848485591 35.0 26.5 40.0 2.0 41.0 46-47 29.55462183729731 35.0 26.5 40.0 2.0 41.0 48-49 29.350616828956632 35.0 26.0 40.0 2.0 41.0 50-51 29.34131257740431 35.0 26.0 40.0 2.0 41.0 52-53 29.181809090598257 35.0 26.0 39.0 2.0 41.0 54-55 29.04973452950131 35.0 26.0 39.0 2.0 41.0 56-57 28.64417663901973 34.0 24.5 39.0 2.0 40.0 58-59 28.347811740613217 34.0 24.0 39.0 2.0 40.0 60-61 27.972468347631857 34.0 23.0 38.5 2.0 40.0 62-63 27.867974608511577 34.0 23.0 38.0 2.0 40.0 64-65 27.646816214896305 33.5 22.5 38.0 2.0 40.0 66-67 27.400765442207252 33.0 23.0 37.0 2.0 40.0 68-69 27.203715437169258 33.0 22.5 37.0 2.0 40.0 70-71 27.0705193551247 33.0 22.5 36.5 2.0 39.0 72-73 27.06549028756505 33.0 23.0 36.0 2.0 39.0 74-75 26.78444769821177 33.0 22.5 36.0 2.0 39.0 76-77 26.500198040054016 33.0 22.0 35.0 2.0 38.5 78-79 26.23970864964521 33.0 21.5 35.0 2.0 37.0 80-81 26.23113921898086 33.0 22.5 35.0 2.0 37.0 82-83 26.064478452667217 33.0 22.0 35.0 2.0 36.0 84-85 25.882346658145952 33.0 21.5 35.0 2.0 36.0 86-87 25.690228591779388 33.0 20.0 35.0 2.0 36.0 88-89 25.495027212730722 33.0 20.0 35.0 2.0 35.0 90-91 25.344356856981797 32.5 19.5 35.0 2.0 35.0 92-93 25.237112796897442 32.0 19.5 35.0 2.0 35.0 94-95 25.196274032969963 32.0 19.0 35.0 2.0 35.0 96-97 25.09049764787722 32.0 18.0 35.0 2.0 35.0 98-99 25.00389347653944 32.0 18.0 35.0 2.0 35.0 100-101 24.93964816346217 32.0 17.5 35.0 2.0 35.0 102-103 23.78926541210032 30.5 11.0 34.0 2.0 35.0 104-105 24.616571459175457 32.0 15.0 34.5 2.0 35.0 106-107 24.681961138156915 32.0 10.0 35.0 2.0 35.0 108-109 24.63980841099862 32.0 8.5 35.0 2.0 35.0 110-111 24.55016782722072 32.0 7.0 35.0 2.0 35.0 112-113 24.527947854192895 32.0 6.0 35.0 2.0 35.0 114-115 24.479576457530925 32.0 2.0 35.0 2.0 35.0 116-117 24.289995391672996 32.0 2.0 35.0 2.0 35.0 118-119 24.15722791732365 32.0 2.0 35.0 2.0 35.0 120-121 24.014143146195316 32.0 2.0 35.0 2.0 35.0 122-123 23.744467511294637 32.0 2.0 35.0 2.0 35.0 124-125 22.706177094708533 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6836980782714583 1101 2 1.3154930124027402 1101 3 1.6099085754523372 1101 4 1.991255290321856 1101 5 1.3685326912190057 1101 6 1.3385147642049802 1101 7 1.2116598120157391 1101 8 1.6480872084023268 1101 9 2.1666341987165865 1101 10-11 2.1111186163485414 1101 12-13 1.891805396388417 1101 14-15 2.264701644226861 1101 16-17 1.9163022422522218 1101 18-19 1.8708154305561315 1101 20-21 2.2593026412661814 1101 22-23 2.0836679471962896 1101 24-25 2.050194804894666 1101 26-27 1.6445063998173737 1101 28-29 1.6382049610657816 1101 30-31 1.8221634621857383 1101 32-33 2.019393369556706 1101 34-35 2.285957393091131 1101 36-37 2.2123785082651004 1101 38-39 2.220958702654155 1101 40-41 2.0389521176734355 1101 42-43 1.9786903374386284 1101 44-45 2.215236133011146 1101 46-47 2.143943560842743 1101 48-49 2.1081971971724407 1101 50-51 2.1010403986200927 1101 52-53 2.042767328055664 1101 54-55 2.074713577409103 1101 56-57 2.17692722075466 1101 58-59 2.2285318822832316 1101 60-61 2.4093118052182767 1101 62-63 2.037791422364421 1101 64-65 2.0357146530745567 1101 66-67 2.140800895420629 1101 68-69 1.9143409416100923 1101 70-71 1.9815702534127055 1101 72-73 1.8596479236855963 1101 74-75 1.8729296084941076 1101 76-77 2.083533204062615 1101 78-79 2.065554992822751 1101 80-81 1.8335802087574997 1101 82-83 1.8609950540778755 1101 84-85 1.7497282722658518 1101 86-87 1.7073164752912433 1101 88-89 1.7472079638041471 1101 90-91 1.7840348436724014 1101 92-93 1.8208925143234502 1101 94-95 1.830996715352601 1101 96-97 1.7467236776739057 1101 98-99 1.69798174712772 1101 100-101 1.717478551164934 1101 102-103 1.7339750293493559 1101 104-105 1.700112128922207 1101 106-107 1.8198272440980006 1101 108-109 1.7774792598012326 1101 110-111 1.9140784549832226 1101 112-113 1.8271676775802952 1101 114-115 1.8330312892751657 1101 116-117 1.8425471106980424 1101 118-119 1.7829774824815665 1101 120-121 1.8252728237224183 1101 122-123 1.8095434541293969 1101 124-125 1.8347618055562336 1102 1 0.5879058470241247 1102 2 0.29282045512253774 1102 3 0.35209492651200236 1102 4 0.8137483868123638 1102 5 0.666958480962716 1102 6 0.6200421065530399 1102 7 0.6124611387526002 1102 8 0.8244757763174064 1102 9 1.2240145361242547 1102 10-11 0.8852700375708409 1102 12-13 1.1654154268243992 1102 14-15 1.3754003186709909 1102 16-17 0.8878577816744837 1102 18-19 1.024407779082015 1102 20-21 1.0754917362012577 1102 22-23 0.9870112000188271 1102 24-25 0.8248697466563115 1102 26-27 0.7664589322147393 1102 28-29 0.5181839381964686 1102 30-31 0.4488151858036886 1102 32-33 0.40647216472787306 1102 34-35 0.7970021846942146 1102 36-37 1.0436414673020202 1102 38-39 1.091318778496106 1102 40-41 0.6247645308007286 1102 42-43 0.7046377930697609 1102 44-45 1.0562030090943182 1102 46-47 0.9311973383643242 1102 48-49 1.0755489396986704 1102 50-51 0.9657926044923002 1102 52-53 1.041307499811424 1102 54-55 0.8200246266169877 1102 56-57 1.1013740853312584 1102 58-59 1.0925880187271346 1102 60-61 1.4702301251987535 1102 62-63 1.2350975805323259 1102 64-65 1.3251724691600693 1102 66-67 1.2686376028844997 1102 68-69 1.109561020383385 1102 70-71 1.2947239548163552 1102 72-73 1.130411472409584 1102 74-75 1.2191974524795768 1102 76-77 1.095041254624416 1102 78-79 1.0016650527834905 1102 80-81 0.6056993477982147 1102 82-83 0.557586946244264 1102 84-85 0.5431295235187861 1102 86-87 0.7005982880292088 1102 88-89 0.41600253142310706 1102 90-91 0.34882429424715156 1102 92-93 0.3314269005945256 1102 94-95 0.615812089906143 1102 96-97 0.7346805142212496 1102 98-99 0.7508994953846084 1102 100-101 0.7496706045427111 1102 102-103 0.5525732666654868 1102 104-105 0.556640742388705 1102 106-107 0.7914861512444453 1102 108-109 0.9258559825085797 1102 110-111 0.6718760517799787 1102 112-113 0.5653663218780949 1102 114-115 0.6762963568147562 1102 116-117 0.9157468433715792 1102 118-119 1.0524941230690672 1102 120-121 0.9471289966402701 1102 122-123 1.0966978419991626 1102 124-125 1.0529929040872474 1103 1 1.0571646610058458 1103 2 0.7206407831539217 1103 3 0.8876652385639225 1103 4 1.4262582186740502 1103 5 0.8780998458259361 1103 6 0.9943184088635064 1103 7 0.8166546807373258 1103 8 1.2179054906765288 1103 9 1.8281914188368873 1103 10-11 1.2549815274207958 1103 12-13 1.3615832472680758 1103 14-15 1.6475471409304632 1103 16-17 1.4640934237964345 1103 18-19 1.150023863499836 1103 20-21 1.5285642701406204 1103 22-23 1.3908820272110702 1103 24-25 1.3922373113035889 1103 26-27 1.1379855013314142 1103 28-29 0.9813175003942405 1103 30-31 1.082166642216329 1103 32-33 1.1471031760722745 1103 34-35 0.9972564651972853 1103 36-37 1.3503441207052376 1103 38-39 1.3809329100596628 1103 40-41 1.5820064304640873 1103 42-43 1.4475549066273956 1103 44-45 1.2674805861672809 1103 46-47 1.5270642604731606 1103 48-49 1.5290636163454785 1103 50-51 1.3646874821411998 1103 52-53 1.3594907358640889 1103 54-55 1.3618115507761992 1103 56-57 1.5080990709106743 1103 58-59 1.6473451238832446 1103 60-61 1.5723985341866964 1103 62-63 1.3735193473559661 1103 64-65 1.1960621883731974 1103 66-67 1.2680351185900811 1103 68-69 1.2186171944799646 1103 70-71 1.3076732413978718 1103 72-73 1.2526157035208847 1103 74-75 1.3317723586461518 1103 76-77 1.3741126299160875 1103 78-79 1.5153152321363415 1103 80-81 1.0215644602037628 1103 82-83 1.1046590948012138 1103 84-85 1.0879484351994826 1103 86-87 1.0603578162811296 1103 88-89 1.1193420278536337 1103 90-91 1.081877915380609 1103 92-93 1.148935217337609 1103 94-95 1.0391410875571232 1103 96-97 1.1419809166848864 1103 98-99 1.084715926771615 1103 100-101 0.959399822608674 1103 102-103 0.9572366126255929 1103 104-105 1.1360512416995974 1103 106-107 0.919864314845686 1103 108-109 1.1973344647770041 1103 110-111 1.3566991110880835 1103 112-113 1.2305804958868478 1103 114-115 1.0938373826164671 1103 116-117 1.328780035799145 1103 118-119 1.3716353141507618 1103 120-121 1.3137799000307133 1103 122-123 1.3368555643609845 1103 124-125 1.2945337523567488 1104 1 1.1365082626889809 1104 2 0.9375130504113294 1104 3 0.9391575892280528 1104 4 1.2871130773187147 1104 5 0.9534418352588219 1104 6 0.7701465581040772 1104 7 0.7158189024702395 1104 8 1.1296373786385274 1104 9 1.6310611826130597 1104 10-11 1.38316855975701 1104 12-13 0.8667541426695458 1104 14-15 1.25844978235164 1104 16-17 0.8650841482987843 1104 18-19 0.12042154445431308 1104 20-21 -4.276783710923624 1104 22-23 -0.922080890289358 1104 24-25 0.3908802596206655 1104 26-27 0.5598111827995105 1104 28-29 0.10021658165263148 1104 30-31 0.8363395448961377 1104 32-33 0.7750815056197347 1104 34-35 1.0762157216577037 1104 36-37 1.213825001122423 1104 38-39 1.266230172790241 1104 40-41 1.222102778633964 1104 42-43 1.180956324651774 1104 44-45 1.3238047032199844 1104 46-47 1.280894284720894 1104 48-49 1.36376480325983 1104 50-51 1.2753845155323056 1104 52-53 1.310937662017345 1104 54-55 1.267647834173367 1104 56-57 1.5037421119217314 1104 58-59 1.4225003267751752 1104 60-61 1.493717895279719 1104 62-63 1.1186792266068153 1104 64-65 1.3138566788397767 1104 66-67 1.2314662001740224 1104 68-69 1.2070410710788444 1104 70-71 1.4272779127575639 1104 72-73 1.256990204435251 1104 74-75 1.2862548859808314 1104 76-77 1.2544525653516843 1104 78-79 1.2376455675709828 1104 80-81 1.0514191955044403 1104 82-83 0.9779115567338224 1104 84-85 0.9129439232496246 1104 86-87 0.8728380258200978 1104 88-89 1.0410882573439757 1104 90-91 0.9899534054721002 1104 92-93 0.6403217599877742 1104 94-95 0.8334485616522791 1104 96-97 0.9281288065727757 1104 98-99 0.8661330614089415 1104 100-101 0.4495879497157702 1104 102-103 0.9114179812558412 1104 104-105 0.9760857690890283 1104 106-107 1.1527254445427069 1104 108-109 1.1411117903430892 1104 110-111 1.2103396408810134 1104 112-113 1.0874086866388453 1104 114-115 1.0301068980085013 1104 116-117 1.086392048581093 1104 118-119 1.0997478342201994 1104 120-121 1.1316921996283504 1104 122-123 0.998784214997098 1104 124-125 1.1486088874437108 1105 1 1.1051072264966209 1105 2 0.8630636574818062 1105 3 0.901456075480148 1105 4 1.4682075796514447 1105 5 0.9166359888035558 1105 6 0.9097401382467503 1105 7 0.7358695636799624 1105 8 1.06868716013107 1105 9 1.5587160199455923 1105 10-11 1.2031873298518754 1105 12-13 1.3743855433593914 1105 14-15 1.57470510011985 1105 16-17 1.309998103354829 1105 18-19 0.8827580922370295 1105 20-21 1.250786791317232 1105 22-23 1.0907042202877761 1105 24-25 1.1190905346298052 1105 26-27 1.0597002611453803 1105 28-29 0.6613740149346654 1105 30-31 1.0648397018452656 1105 32-33 1.0073298483572586 1105 34-35 0.9271242128424859 1105 36-37 1.0965672778946463 1105 38-39 1.0265979751415237 1105 40-41 1.1436110798027528 1105 42-43 1.4053868326456076 1105 44-45 1.6416568097470616 1105 46-47 1.87144071559565 1105 48-49 1.4759042714265007 1105 50-51 1.6279794351200323 1105 52-53 1.5456056827120257 1105 54-55 1.6145243642450744 1105 56-57 1.9814145490664323 1105 58-59 1.5517506221713013 1105 60-61 1.9071859715354833 1105 62-63 1.7407597176297216 1105 64-65 1.477786123553397 1105 66-67 1.7219426146028418 1105 68-69 1.428116085105831 1105 70-71 1.495067039117835 1105 72-73 1.2971558741741838 1105 74-75 1.529831865973371 1105 76-77 1.4937451622746067 1105 78-79 1.347968499971298 1105 80-81 1.308928555031688 1105 82-83 1.3754073249405607 1105 84-85 1.3053017038248989 1105 86-87 1.3554091077336885 1105 88-89 1.469387843880547 1105 90-91 1.4796649891197902 1105 92-93 1.4256698571117568 1105 94-95 1.3070676189585804 1105 96-97 1.497393090675125 1105 98-99 1.287406303460223 1105 100-101 1.3799859333296247 1105 102-103 1.3697659886946063 1105 104-105 1.4853567959883947 1105 106-107 1.5287860360203496 1105 108-109 1.6619089624880274 1105 110-111 1.5378077069287066 1105 112-113 1.558018185178181 1105 114-115 1.5827907189778543 1105 116-117 1.6741528602979194 1105 118-119 1.6073595934832063 1105 120-121 1.5159804982232465 1105 122-123 1.6147631947512089 1105 124-125 1.219700097672785 1106 1 -0.6248624935951845 1106 2 -0.24343312703723896 1106 3 -0.3955005273393404 1106 4 -0.5667071801953085 1106 5 -1.1641091764139553 1106 6 -0.5173671654239342 1106 7 -0.5483315524141332 1106 8 -0.15160734012478017 1106 9 -0.2185012142682936 1106 10-11 -2.6452585000759754 1106 12-13 -0.7055702029367978 1106 14-15 -0.46730066985136887 1106 16-17 -0.6914011611610817 1106 18-19 -3.4589659901420156 1106 20-21 -6.015042176461613 1106 22-23 -6.8355776618316995 1106 24-25 -6.791089878129974 1106 26-27 -5.590560893009716 1106 28-29 -2.17344843240976 1106 30-31 -4.025353860356724 1106 32-33 -4.571038954792176 1106 34-35 -3.89443594635825 1106 36-37 -6.325649637133619 1106 38-39 -6.766796252273455 1106 40-41 -2.989846333798475 1106 42-43 -1.559891238347305 1106 44-45 -0.7103682345116695 1106 46-47 -0.47768690235474764 1106 48-49 -0.30039065457772907 1106 50-51 -0.509296205035632 1106 52-53 -0.6855527524842451 1106 54-55 -0.5785701177141505 1106 56-57 -0.47530664556240154 1106 58-59 -0.28154570322310946 1106 60-61 -0.004486822489763398 1106 62-63 -0.2714770924643837 1106 64-65 -0.5300378825226915 1106 66-67 -0.813909847248194 1106 68-69 -0.6645274780512018 1106 70-71 -0.423218363593886 1106 72-73 -0.4593923947339178 1106 74-75 -0.5583070330523832 1106 76-77 -0.6032442890176704 1106 78-79 -0.6418444522292717 1106 80-81 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-1.7370264453958875 2216 80-81 -1.797482629403497 2216 82-83 -1.9976907146724514 2216 84-85 -1.8224377232866935 2216 86-87 -2.1957306800692997 2216 88-89 -2.328174299583967 2216 90-91 -2.422634531084107 2216 92-93 -2.097654252548633 2216 94-95 -5.4936924146939425 2216 96-97 -7.95046360616924 2216 98-99 -4.236393061764954 2216 100-101 -3.137389882935409 2216 102-103 -4.359032415887658 2216 104-105 -7.390372973659943 2216 106-107 -3.4767558603938227 2216 108-109 -6.314904551206013 2216 110-111 -3.4305342309131923 2216 112-113 -2.679572822603781 2216 114-115 -2.936990363261433 2216 116-117 -2.511259101657622 2216 118-119 -2.770659020474188 2216 120-121 -3.6450114331268075 2216 122-123 -7.118167662975328 2216 124-125 -5.3730139512044275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 285369.0 3 44814.0 4 26919.0 5 21737.0 6 21367.0 7 23649.0 8 25945.0 9 27078.0 10 28176.0 11 28985.0 12 29287.0 13 29318.0 14 28277.0 15 24568.0 16 21019.0 17 19085.0 18 17430.0 19 16884.0 20 17203.0 21 18994.0 22 23990.0 23 32718.0 24 44079.0 25 56437.0 26 68483.0 27 79183.0 28 90977.0 29 103431.0 30 117450.0 31 134217.0 32 159800.0 33 196375.0 34 244019.0 35 309214.0 36 401651.0 37 388567.0 38 98004.0 39 188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55130325861596 18.376543101448416 13.794044374936778 24.278109264998847 2 15.301549577646565 19.918138375988388 39.626129131066975 25.15418291529807 3 18.812799790770786 24.552920884598826 29.017627682187126 27.61665164244326 4 11.821912524997744 16.09763938948287 37.28510113226237 34.79534695325702 5 14.402332520386244 37.35831954875381 33.52168558564886 14.71766234521108 6 32.856005979199885 35.608132438067166 17.197764457045682 14.338097125687261 7 28.959696492158134 31.82941058283285 20.71036158052592 18.500531344483097 8 26.049450107206855 35.3653717321275 19.655800335630378 18.929377825035267 9 25.843499711318845 16.484299255238927 20.120713508462533 37.55148752497969 10-11 24.87524137555312 26.413704078111856 27.211393531437842 21.499661014897185 12-13 25.672370787309056 23.442447045665745 27.73430585105337 23.15087631597183 14-15 22.90204219353101 24.904805063344767 24.993274394128072 27.199878348996155 16-17 23.30847786121782 28.363444433667027 25.176610444382675 23.151467260732474 18-19 23.277320995366 27.131513386307184 26.938656987076126 22.652508631250694 20-21 23.691196004430903 26.315742430742905 27.070047290435017 22.923014274391175 22-23 23.59050388978089 26.1801402902556 26.815724117519153 23.41363170244436 24-25 23.48563714242655 26.340126848051675 26.7259275892394 23.448308420282373 26-27 23.331788048208054 26.6692256669035 26.44619321228465 23.55279307260379 28-29 23.264740885583333 26.74305095987971 26.513350381588612 23.47885777294835 30-31 23.290675360247203 26.400245218036478 26.730157884831033 23.578921536885282 32-33 23.17528527160471 26.595700095757515 26.48016772467952 23.748846907958253 34-35 23.56556164644326 26.414819968595456 26.609445242310887 23.410173142650397 36-37 23.609940677286556 26.16258750516294 26.471047459683707 23.756424357866806 38-39 23.38055254420134 26.38458704435983 26.63782323094393 23.597037180494898 40-41 23.533342191854377 26.3426486029517 26.627730540740064 23.49627866445385 42-43 23.53353811831742 26.42366342390991 26.302205682866532 23.740592774906137 44-45 23.415696752055098 26.410564480232342 26.47347786044225 23.700260907270312 46-47 23.58669352886496 26.559701013807928 26.16030837868195 23.693297078645156 48-49 23.691574262671953 26.577429468792147 25.962317387182292 23.7686788813536 50-51 23.533847726273756 26.69019992933969 25.99621581504853 23.779736529338024 52-53 23.919710916205705 26.44445816307355 25.914712291599145 23.721118629121605 54-55 23.618730800312353 26.354874002841814 26.061886561326958 23.96450863551887 56-57 23.492592607722536 26.671862288485315 26.091916511168694 23.74362859262346 58-59 23.70243761682211 26.345671947395573 25.97886205409001 23.97302838169231 60-61 23.584886244913918 26.30062019614324 26.271293138618617 23.843200420324226 62-63 23.60284985056728 26.473171482309322 26.233116856491783 23.69086181063161 64-65 23.542865909621145 26.3785941733095 26.024158400382003 24.054381516687346 66-67 23.413262676846735 26.66806268907202 25.978242022473335 23.940432611607907 68-69 23.386384313141644 26.503157745983202 26.216652497245168 23.893805443629986 70-71 23.264619051574368 26.36099046484757 26.364818263531987 24.009572220046078 72-73 23.498305226293592 26.432100424041398 26.021569407050087 24.04802494261492 74-75 23.354260146675294 26.7207054115299 26.03802713042131 23.88700731137349 76-77 23.54948165134422 26.408153532514543 26.14293465317892 23.899430162962318 78-79 23.390402076797066 26.464984161638146 26.2486158267407 23.89599793482409 80-81 23.480151670894372 26.667270840753126 26.072799850640905 23.7797776377116 82-83 23.479697936719944 26.587659899211374 26.085199492365202 23.847442671703476 84-85 23.41070690740059 26.63933378328349 25.940685555192957 24.00927375412297 86-87 23.574721309277898 26.37271095065683 26.159656146915523 23.892911593149748 88-89 23.381844755380182 26.4532547386768 26.049090391924278 24.11581011401874 90-91 23.300362159923782 26.54928632390505 26.19694558341759 23.953405932753576 92-93 23.49468525550065 26.412957092460985 26.117479175463803 23.974878476574563 94-95 23.401679108965673 26.253510625566506 26.152021528760468 24.192788736707357 96-97 23.558835588355883 26.187627558932412 26.09276683172736 24.160770020984344 98-99 23.247353061455755 26.259282497003987 26.134479501611064 24.358884939929197 100-101 23.156211864332416 26.202571824790088 26.378693741521836 24.262522569355664 102-103 23.363723301360853 26.016245402433707 26.369103083673245 24.250928212532187 104-105 23.288965315967992 26.23718897894757 26.26416472543668 24.20968097964776 106-107 23.16186782931698 26.146045993434523 26.440990140892378 24.25109603635612 108-109 23.21899281071867 26.09101379926263 26.222396444256706 24.467596945761994 110-111 23.049914715398764 26.21638353954243 26.461011305618438 24.27269043944037 112-113 23.211703680056644 26.195872787007275 26.455815037192426 24.136608495743655 114-115 23.132664571945913 26.34155729093541 26.220521808173284 24.305256328945394 116-117 22.859770248501814 26.282016658171305 26.49737370014535 24.36083939318153 118-119 23.082723511797415 26.334698220039265 26.077677505876316 24.504900762287008 120-121 22.950307486028247 26.368557174426872 26.331125671505124 24.35000966803976 122-123 22.920314805854694 26.302519965855385 26.393903110264656 24.383262118025264 124-125 23.043073313957 26.436494876664707 26.054634479207174 24.465797330171117 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 604.0 1 651.0 2 820.5 3 854.0 4 667.5 5 544.5 6 514.5 7 558.0 8 679.0 9 793.0 10 809.0 11 822.0 12 919.5 13 1053.5 14 1169.5 15 1248.5 16 1364.5 17 1533.0 18 1775.0 19 2107.0 20 2536.5 21 3116.0 22 4007.0 23 5302.5 24 7118.5 25 9640.0 26 12658.0 27 16402.0 28 20636.0 29 24917.5 30 30032.0 31 36293.5 32 42537.5 33 47585.5 34 52135.5 35 56306.0 36 61377.5 37 67786.5 38 73815.5 39 77964.5 40 82191.5 41 89112.0 42 95171.0 43 101076.0 44 107416.5 45 113338.5 46 118063.5 47 121582.5 48 127041.0 49 130748.5 50 131063.0 51 130427.5 52 129869.5 53 130751.5 54 129491.0 55 123145.0 56 115460.0 57 107083.5 58 98812.5 59 89070.0 60 76185.0 61 63319.0 62 51467.0 63 39890.5 64 30495.5 65 24011.5 66 18781.0 67 14780.5 68 11171.5 69 8254.5 70 6086.0 71 4557.0 72 3556.0 73 2825.5 74 2195.0 75 1631.5 76 1116.5 77 703.5 78 465.5 79 309.0 80 217.0 81 151.0 82 95.5 83 70.5 84 59.0 85 46.5 86 35.0 87 29.0 88 19.0 89 14.0 90 15.5 91 12.0 92 9.5 93 8.0 94 6.5 95 5.5 96 4.0 97 3.5 98 3.5 99 3.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08874736110493339 2 0.030833126820977542 3 0.04033420809849172 4 0.04808031257952239 5 0.05080355243613473 6 0.0029350474010155264 7 0.001906267899628641 8 0.0018457514583705887 9 0.006142418787692287 10-11 0.02744420611052662 12-13 0.006051644125805209 14-15 0.010650893661417167 16-17 0.019713230739810468 18-19 0.018608805686851016 20-21 0.1340741756072144 22-23 0.015840178499295136 24-25 0.013949039709981008 26-27 0.006384484552724496 28-29 0.004538733094353907 30-31 0.0022088501059189013 32-33 0.005612899926684331 34-35 0.01780696284018183 36-37 0.002481174091580136 38-39 0.00800329935637739 40-41 0.033284042691928646 42-43 0.013480037290231102 44-45 0.10658458216574425 46-47 0.0036007282548540997 48-49 0.008653851099901449 50-51 0.010423957006699472 52-53 0.011437607397771844 54-55 0.009334661064054535 56-57 0.005491867044168227 58-59 0.013631328393376234 60-61 0.023329088104979082 62-63 0.006732454089958296 64-65 0.005991127684547157 66-67 0.007867137363546772 68-69 0.007125810958135633 70-71 0.003494824482652508 72-73 6.354226332095469E-4 74-75 0.0010892959426449377 76-77 0.00780662092228872 78-79 0.017186669317286792 80-81 0.0030258220629026046 82-83 0.004508474873724881 84-85 0.0023147538781204922 86-87 0.0031317258351041956 88-89 0.00505312284504735 90-91 0.003978956012716925 92-93 0.002163462774975362 94-95 0.0029804347319590655 96-97 0.0012708452664190939 98-99 0.0023752703193785445 100-101 0.0022391083265479272 102-103 4.084859784918516E-4 104-105 0.002012171671830232 106-107 7.261972950966251E-4 108-109 0.0011044250529594507 110-111 0.002118075444031823 112-113 0.0034645662620234824 114-115 0.002148333664660849 116-117 0.004311796439636212 118-119 0.002874530959757474 120-121 0.005567512595740793 122-123 0.003630986475483125 124-125 0.05193823570972321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3304887.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.65824224130871 #Duplication Level Percentage of deduplicated Percentage of total 1 79.22855014828527 46.47407487025792 2 10.488842383506103 12.305141147252137 3 4.014502423453082 7.064509668996953 4 2.0146634015615885 4.727064553739947 5 1.1015928304783522 3.2308749550744054 6 0.705608911208081 2.4833867064761823 7 0.4718707522677893 1.9375376225178789 8 0.35148538416903924 1.6494011847093564 9 0.25978892160834377 1.371488534577953 >10 1.2687299985151623 12.849924459270005 >50 0.06691777021288944 2.6801519801068534 >100 0.026324423303243485 2.7248344398123736 >500 0.0010157322474263154 0.40195270115596027 >1k 1.0691918376452722E-4 0.09965717605207693 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.0258220629026046E-5 0.0 10-11 0.0 0.0 0.0 3.0258220629026046E-5 0.0 12-13 0.0 0.0 0.0 3.0258220629026046E-5 0.0 14-15 0.0 0.0 0.0 4.538733094353907E-5 3.0258220629026046E-5 16-17 0.0 0.0 1.5129110314513023E-5 9.077466188707814E-5 4.538733094353907E-5 18-19 0.0 0.0 3.0258220629026046E-5 9.077466188707814E-5 6.051644125805209E-5 20-21 0.0 0.0 3.0258220629026046E-5 1.2103288251610418E-4 6.051644125805209E-5 22-23 0.0 0.0 3.0258220629026046E-5 2.723239856612344E-4 1.0590377220159116E-4 24-25 0.0 0.0 3.0258220629026046E-5 6.051644125805209E-4 1.2103288251610418E-4 26-27 0.0 0.0 3.0258220629026046E-5 7.715846260401642E-4 1.2103288251610418E-4 28-29 0.0 1.5129110314513023E-5 3.0258220629026046E-5 0.0013011034870481198 1.2103288251610418E-4 30-31 0.0 3.0258220629026046E-5 3.0258220629026046E-5 0.003267887827934813 1.2103288251610418E-4 32-33 1.5129110314513023E-5 3.0258220629026046E-5 3.0258220629026046E-5 0.006883745193103426 1.5129110314513023E-4 34-35 4.538733094353907E-5 3.0258220629026046E-5 3.0258220629026046E-5 0.01225457935475555 1.8154932377415628E-4 36-37 6.051644125805209E-5 3.0258220629026046E-5 6.051644125805209E-5 0.021589240418810086 1.966784340886693E-4 38-39 6.051644125805209E-5 3.0258220629026046E-5 6.051644125805209E-5 0.03720248226338752 2.118075444031823E-4 40-41 6.051644125805209E-5 3.0258220629026046E-5 6.051644125805209E-5 0.056673647238165786 2.118075444031823E-4 42-43 6.051644125805209E-5 3.0258220629026046E-5 6.051644125805209E-5 0.07561529335193609 2.118075444031823E-4 44-45 6.051644125805209E-5 3.0258220629026046E-5 6.051644125805209E-5 0.09908054344974579 2.118075444031823E-4 46-47 7.564555157256511E-5 3.0258220629026046E-5 6.051644125805209E-5 0.128960536320909 2.118075444031823E-4 48-49 9.077466188707814E-5 3.0258220629026046E-5 6.051644125805209E-5 0.16359106983082933 2.4206576503220837E-4 50-51 9.077466188707814E-5 3.0258220629026046E-5 6.051644125805209E-5 0.2021400429122085 2.4206576503220837E-4 52-53 1.0590377220159116E-4 3.0258220629026046E-5 6.051644125805209E-5 0.24090082353799086 2.4206576503220837E-4 54-55 1.2103288251610418E-4 3.0258220629026046E-5 6.051644125805209E-5 0.2864999620259331 2.4206576503220837E-4 56-57 1.361619928306172E-4 3.0258220629026046E-5 6.051644125805209E-5 0.33917952414106745 2.4206576503220837E-4 58-59 1.5129110314513023E-4 3.0258220629026046E-5 6.051644125805209E-5 0.3987277023389907 2.4206576503220837E-4 60-61 1.5129110314513023E-4 3.0258220629026046E-5 6.051644125805209E-5 0.4659463394663721 2.4206576503220837E-4 62-63 1.5129110314513023E-4 3.0258220629026046E-5 6.051644125805209E-5 0.5360546366638255 2.4206576503220837E-4 64-65 1.5129110314513023E-4 3.0258220629026046E-5 6.051644125805209E-5 0.6095367254614152 2.4206576503220837E-4 66-67 1.5129110314513023E-4 3.0258220629026046E-5 6.051644125805209E-5 0.696408076887349 2.4206576503220837E-4 68-69 1.5129110314513023E-4 3.0258220629026046E-5 6.051644125805209E-5 0.7978790197667878 2.4206576503220837E-4 70-71 1.6642021345964327E-4 3.0258220629026046E-5 6.051644125805209E-5 0.9150691082630057 2.4206576503220837E-4 72-73 1.8154932377415628E-4 3.0258220629026046E-5 6.051644125805209E-5 1.0374030942661578 2.723239856612344E-4 74-75 1.8154932377415628E-4 3.0258220629026046E-5 6.051644125805209E-5 1.1646842993421562 2.723239856612344E-4 76-77 1.8154932377415628E-4 3.0258220629026046E-5 6.051644125805209E-5 1.3045529241998288 2.723239856612344E-4 78-79 1.966784340886693E-4 3.0258220629026046E-5 6.051644125805209E-5 1.4710336541007303 2.723239856612344E-4 80-81 2.118075444031823E-4 6.051644125805209E-5 6.051644125805209E-5 1.657878166484966 2.8745309597574744E-4 82-83 2.118075444031823E-4 6.051644125805209E-5 6.051644125805209E-5 1.8571285493271026 3.328404269192865E-4 84-85 2.118075444031823E-4 6.051644125805209E-5 6.051644125805209E-5 2.06288444960448 3.328404269192865E-4 86-87 2.118075444031823E-4 6.051644125805209E-5 6.051644125805209E-5 2.2789130157854114 3.328404269192865E-4 88-89 2.118075444031823E-4 6.051644125805209E-5 6.051644125805209E-5 2.524503863520901 3.328404269192865E-4 90-91 2.4206576503220837E-4 9.077466188707814E-5 6.051644125805209E-5 2.8053304091788918 3.328404269192865E-4 92-93 2.4206576503220837E-4 9.077466188707814E-5 6.051644125805209E-5 3.1038277556842333 3.328404269192865E-4 94-95 2.4206576503220837E-4 9.077466188707814E-5 6.051644125805209E-5 3.405245020480277 3.328404269192865E-4 96-97 2.4206576503220837E-4 9.077466188707814E-5 6.051644125805209E-5 3.7173736953789946 3.328404269192865E-4 98-99 2.4206576503220837E-4 9.077466188707814E-5 6.051644125805209E-5 4.05614473354157 3.328404269192865E-4 100-101 2.4206576503220837E-4 9.077466188707814E-5 6.051644125805209E-5 4.438563254961515 3.328404269192865E-4 102-103 2.4206576503220837E-4 9.077466188707814E-5 6.051644125805209E-5 4.85272264982131 3.328404269192865E-4 104-105 2.723239856612344E-4 1.0590377220159116E-4 6.051644125805209E-5 5.26499090589179 3.328404269192865E-4 106-107 2.723239856612344E-4 1.2103288251610418E-4 6.051644125805209E-5 5.685277590428962 3.6309864754831256E-4 108-109 2.723239856612344E-4 1.2103288251610418E-4 6.051644125805209E-5 6.128847975740169 4.084859784918516E-4 110-111 2.723239856612344E-4 1.2103288251610418E-4 6.051644125805209E-5 6.618728567724101 4.236150888063646E-4 112-113 2.723239856612344E-4 1.2103288251610418E-4 6.051644125805209E-5 7.151485058339363 4.236150888063646E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6155 0.0 50.289726 1 GGTATCA 3475 0.0 48.30569 1 ATCAACG 7275 0.0 39.913506 3 TCAACGC 7455 0.0 39.131943 4 CAACGCA 7670 0.0 38.11149 5 AACGCAG 7570 0.0 37.418823 6 TATCAAC 8415 0.0 36.910458 2 GTACATG 10910 0.0 34.154945 1 TACATGG 12050 0.0 30.81276 2 ACGCAGA 9230 0.0 30.56109 7 CGCAGAG 9325 0.0 29.804373 8 ACATGGG 11560 0.0 29.699629 3 CATGGGG 6960 0.0 23.865906 4 GCAGAGT 10970 0.0 23.273548 9 AGAGTAC 9525 0.0 23.081434 10-11 CAGAGTA 10185 0.0 20.65103 10-11 CATGGGA 6425 0.0 20.57135 4 ACGCATA 395 3.255991E-10 19.586561 8 GGACCGT 610 0.0 19.510986 6 GAGTACA 8455 0.0 19.431194 1 >>END_MODULE