FastQCFastQC Report
Thu 26 May 2016
SRR1364502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304887
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54600.1652098846344822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51800.15673758285835493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47100.067.361931
GTATCAA86300.050.9307941
ATCAACG109400.038.7744143
TATCAAC115700.038.1564032
TCAACGC110250.038.151244
CAACGCA112650.036.9145135
AACGCAG114150.036.1167346
CGCAGAG137800.029.6497218
GTACATG110350.029.4542481
ACGCAGA138750.029.3274467
TACATGG116600.027.0296042
ACATGGG113900.026.0488283
GCAGAGT152150.025.0943649
GAGTACT88850.024.59912312-13
AGAGTAC134750.023.56851210-11
GTACTTT94300.023.46141614-15
CAGAGTA143950.023.44627810-11
GTGGTAT23050.021.9923571
GAGTACA89550.020.7118341
AGTACTT95600.020.5604812-13
Produced by FastQC (version 0.11.5)