FastQCFastQC Report
Thu 26 May 2016
SRR1364501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476645
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.1412661811064948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16100.10903094514930806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30500.053.279361
GTATCAA53450.048.6664731
ATCAACG64450.038.5872233
TCAACGC65350.038.56724
TATCAAC68350.037.95232
CAACGCA66900.037.7627035
AACGCAG66800.037.442126
GTACATG82950.035.879781
TACATGG85650.033.204022
ACGCAGA76250.031.7875397
CGCAGAG74750.031.313238
ACATGGG87500.029.646173
GCAGAGT78950.027.1579449
GAGTACT30850.026.31960112-13
CATGGGA52350.026.1778814
TACCTGG13200.023.8886742
GTACTTT35650.023.647814-15
CAGAGTA80650.023.43142710-11
ATGGGAG23950.023.3854455
ACGCATA2302.3421671E-723.305658
Produced by FastQC (version 0.11.5)