##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364501_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1476645 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.68111089666101 31.0 26.0 31.0 10.0 34.0 2 26.93742504122521 31.0 26.0 31.0 10.0 34.0 3 26.985470441439887 31.0 26.0 31.0 10.0 34.0 4 29.82522474934734 35.0 28.0 37.0 10.0 37.0 5 30.55263181062476 35.0 30.0 37.0 10.0 37.0 6 30.662998892760278 35.0 31.0 37.0 11.0 37.0 7 30.475192073924337 35.0 30.0 37.0 10.0 37.0 8 30.085601481737317 35.0 30.0 37.0 10.0 37.0 9 30.756632095053313 35.0 29.0 39.0 8.0 39.0 10-11 30.855165933585933 36.0 29.5 39.0 8.0 39.0 12-13 30.70082856746205 36.0 29.0 39.0 8.0 39.0 14-15 31.417080611792272 37.0 29.0 40.0 7.5 41.0 16-17 31.707174710238412 37.0 30.0 40.0 7.0 41.0 18-19 31.693578009609624 37.0 30.0 40.0 3.5 41.0 20-21 31.493248546536236 37.0 29.5 40.0 2.0 41.0 22-23 31.561525281973665 37.0 29.5 40.0 2.0 41.0 24-25 31.592012975359683 37.0 30.0 40.0 2.0 41.0 26-27 31.717499128091042 37.0 30.0 40.0 2.0 41.0 28-29 31.673088657057043 37.0 30.0 40.0 2.0 41.0 30-31 31.343403458515755 37.0 30.0 40.0 2.0 41.0 32-33 31.340696646790526 37.0 30.0 40.0 2.0 41.0 34-35 31.0577308696403 36.5 29.5 40.0 2.0 41.0 36-37 30.83307328437099 36.0 29.0 40.0 2.0 41.0 38-39 30.694886719556834 36.0 28.5 40.0 2.0 41.0 40-41 30.70867947272364 36.0 29.0 40.0 2.0 41.0 42-43 30.572202188068225 36.0 28.0 40.0 2.0 41.0 44-45 30.2072620704367 36.0 27.5 40.0 2.0 41.0 46-47 29.954140297769605 35.5 27.0 40.0 2.0 41.0 48-49 29.698185413555727 35.0 26.5 40.0 2.0 41.0 50-51 29.68791889722987 35.0 27.0 40.0 2.0 41.0 52-53 29.490759796701305 35.0 26.5 40.0 2.0 41.0 54-55 29.375618378147763 35.0 26.5 40.0 2.0 41.0 56-57 28.958116541213357 35.0 25.0 39.0 2.0 41.0 58-59 28.740542581324554 34.5 25.0 39.0 2.0 41.0 60-61 28.319859207866482 34.0 23.5 39.0 2.0 40.0 62-63 28.288983472669464 34.0 24.5 39.0 2.0 40.0 64-65 28.10367623904188 34.0 24.0 38.0 2.0 40.0 66-67 27.854014675158894 34.0 23.5 38.0 2.0 40.0 68-69 27.682134839450242 34.0 23.5 37.5 2.0 40.0 70-71 27.51453531485225 34.0 23.5 37.0 2.0 40.0 72-73 27.485291996383694 34.0 24.5 37.0 2.0 39.5 74-75 27.2026651632586 33.5 24.0 36.0 2.0 39.0 76-77 26.896591259239695 33.0 23.0 36.0 2.0 39.0 78-79 26.556484463090314 33.0 22.0 35.5 2.0 37.5 80-81 26.558968133843948 33.0 23.5 35.0 2.0 37.0 82-83 26.35220178174172 33.0 23.0 35.0 2.0 37.0 84-85 26.16541077916493 33.0 23.0 35.0 2.0 36.0 86-87 25.948181519593405 33.0 22.5 35.0 2.0 36.0 88-89 25.712607634197795 33.0 20.5 35.0 2.0 36.0 90-91 25.536691283280682 33.0 20.0 35.0 2.0 35.5 92-93 25.429652353815577 33.0 20.0 35.0 2.0 35.0 94-95 25.366826827030195 33.0 20.0 35.0 2.0 35.0 96-97 25.22542791259917 33.0 18.5 35.0 2.0 35.0 98-99 25.127779865844534 33.0 18.0 35.0 2.0 35.0 100-101 25.081282908214227 33.0 18.0 35.0 2.0 35.0 102-103 23.907227532683887 30.5 11.0 34.0 2.0 35.0 104-105 24.688926925564374 32.0 12.5 35.0 2.0 35.0 106-107 24.775986780844413 32.5 10.0 35.0 2.0 35.0 108-109 24.728652113405726 32.5 8.5 35.0 2.0 35.0 110-111 24.614974486081625 32.0 7.0 35.0 2.0 35.0 112-113 24.608241655915943 32.5 4.5 35.0 2.0 35.0 114-115 24.560281245661617 33.0 2.0 35.0 2.0 35.0 116-117 24.35199590964653 32.5 2.0 35.0 2.0 35.0 118-119 24.21246812876487 32.0 2.0 35.0 2.0 35.0 120-121 24.084031707011505 32.0 2.0 35.0 2.0 35.0 122-123 23.82496233014706 32.0 2.0 35.0 2.0 35.0 124-125 22.844849303657952 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.3126547615627118 1101 2 1.8110415231810926 1101 3 2.09764835774876 1101 4 2.5251020612696315 1101 5 2.0733637421459434 1101 6 2.020711712331071 1101 7 1.8845728960500345 1101 8 2.360009953813659 1101 9 2.751353694175055 1101 10-11 2.7367463796925477 1101 12-13 2.602399492245027 1101 14-15 2.915485794813282 1101 16-17 2.6771274967397183 1101 18-19 2.7052808152728893 1101 20-21 3.021817172635835 1101 22-23 2.874053310681713 1101 24-25 2.8591945995334775 1101 26-27 2.5213809596097647 1101 28-29 2.4126430582514544 1101 30-31 2.6521936933302364 1101 32-33 2.817415381450708 1101 34-35 3.0466887276211914 1101 36-37 3.20940387705598 1101 38-39 3.152707203592982 1101 40-41 2.888447417646958 1101 42-43 2.7691888586834423 1101 44-45 2.9065577034178602 1101 46-47 2.895089336859378 1101 48-49 2.8832269031144406 1101 50-51 2.8512232427512174 1101 52-53 2.6689583181079293 1101 54-55 2.8091308597922158 1101 56-57 3.164600042611383 1101 58-59 3.0701232289257554 1101 60-61 3.1715444250307314 1101 62-63 2.9130520560910433 1101 64-65 2.901025644036892 1101 66-67 2.914461483244615 1101 68-69 2.767751475611938 1101 70-71 2.8116337733767622 1101 72-73 2.6573062142075656 1101 74-75 2.6145233003983606 1101 76-77 2.7767072052655095 1101 78-79 2.8380086884806524 1101 80-81 2.6451223649574764 1101 82-83 2.6387566611376734 1101 84-85 2.441880677884214 1101 86-87 2.454288478853872 1101 88-89 2.6347933299014237 1101 90-91 2.7165068056337986 1101 92-93 2.6638772733900105 1101 94-95 2.664195977785674 1101 96-97 2.679825643919532 1101 98-99 2.633071385213256 1101 100-101 2.5413614101551403 1101 102-103 2.5255371530085924 1101 104-105 2.5611307254948876 1101 106-107 2.668005946047707 1101 108-109 2.726853259226502 1101 110-111 2.88015165161249 1101 112-113 2.7903408878310856 1101 114-115 2.6471454396304317 1101 116-117 2.789338823494905 1101 118-119 2.8532374096076687 1101 120-121 2.869496445448938 1101 122-123 2.939205879534203 1101 124-125 2.916032213074221 1102 1 1.0700578837170838 1102 2 0.8814499229056274 1102 3 1.0147261276571449 1102 4 1.5119395499687158 1102 5 1.2387545336509156 1102 6 1.259613148730967 1102 7 1.2130857276932652 1102 8 1.341017473616784 1102 9 1.7044638540687878 1102 10-11 1.3675284114754618 1102 12-13 1.6193884051047434 1102 14-15 1.8544689894893658 1102 16-17 1.4384961400745375 1102 18-19 1.405014570507209 1102 20-21 1.7176378315324712 1102 22-23 1.4273432653474778 1102 24-25 1.237099792571133 1102 26-27 1.3199296278147656 1102 28-29 1.0092363410175977 1102 30-31 0.9777968442060789 1102 32-33 0.8702180094652334 1102 34-35 1.3920540043427643 1102 36-37 1.6220536856339045 1102 38-39 1.5897612864478958 1102 40-41 1.1840072578511425 1102 42-43 1.2384279192302614 1102 44-45 1.558303552675838 1102 46-47 1.459930503864939 1102 48-49 1.5402892841896012 1102 50-51 1.3974537945624625 1102 52-53 1.421163425307551 1102 54-55 1.2622918126269873 1102 56-57 1.3681134862177586 1102 58-59 1.4092872791099964 1102 60-61 1.8549605909755513 1102 62-63 1.7053162707709788 1102 64-65 1.6261220078334766 1102 66-67 1.6543783704900008 1102 68-69 1.3993747527316955 1102 70-71 1.569438547342056 1102 72-73 1.3913500911491212 1102 74-75 1.4985242583246716 1102 76-77 1.4794430477021372 1102 78-79 1.3846188614295976 1102 80-81 0.9760555108396964 1102 82-83 0.9830002094142216 1102 84-85 0.9768453961690184 1102 86-87 1.033853683119009 1102 88-89 0.7294236388856987 1102 90-91 0.7819507563373129 1102 92-93 0.8212301329713938 1102 94-95 1.0726724017015243 1102 96-97 1.1147980321424242 1102 98-99 1.2345411982952328 1102 100-101 1.1538661426701324 1102 102-103 0.9093012811977381 1102 104-105 0.957729352739161 1102 106-107 1.068051557700791 1102 108-109 1.2127821866427766 1102 110-111 1.055797109540432 1102 112-113 0.9129993128500971 1102 114-115 0.9605125914070847 1102 116-117 1.143732188508892 1102 118-119 1.244748787226463 1102 120-121 1.1515390397157397 1102 122-123 1.3141581464088858 1102 124-125 1.1995722811571632 1103 1 1.5102882096351422 1103 2 1.2676818354696344 1103 3 1.5513853528231856 1103 4 1.864988762218669 1103 5 1.5250490597223916 1103 6 1.5510970560760882 1103 7 1.424575961953014 1103 8 1.7977051535076853 1103 9 2.5129607692881315 1103 10-11 1.9638306904760015 1103 12-13 2.2324336507398286 1103 14-15 2.361053242356146 1103 16-17 2.158222837031719 1103 18-19 1.9414418634400796 1103 20-21 2.3995468714805384 1103 22-23 2.2465225858434046 1103 24-25 2.1775210732960097 1103 26-27 1.8738583795440107 1103 28-29 1.853499583282698 1103 30-31 2.0796347637329724 1103 32-33 2.080483018676958 1103 34-35 1.792248974937369 1103 36-37 2.225981044130659 1103 38-39 2.2070603987129402 1103 40-41 2.142721681249583 1103 42-43 2.226224022113108 1103 44-45 2.2464927929871337 1103 46-47 2.3685516626409893 1103 48-49 2.5190433081728685 1103 50-51 2.324862283773925 1103 52-53 2.295317651227574 1103 54-55 2.3077452032631527 1103 56-57 2.445333397313135 1103 58-59 2.7240656240153562 1103 60-61 2.5618404549960587 1103 62-63 2.360518273091941 1103 64-65 2.0078013283294034 1103 66-67 2.1671420192511697 1103 68-69 2.01089113058946 1103 70-71 2.327142713271126 1103 72-73 2.23339809374545 1103 74-75 2.2330589678438884 1103 76-77 2.259213557723573 1103 78-79 2.3338236534937415 1103 80-81 1.9466980532759202 1103 82-83 1.929048510326215 1103 84-85 1.9313457010410708 1103 86-87 1.8735840024806798 1103 88-89 2.0165836082018025 1103 90-91 2.0542330514665714 1103 92-93 2.0789035658307 1103 94-95 1.8259055206409585 1103 96-97 1.9815935318375502 1103 98-99 1.9134793748325407 1103 100-101 1.9044640033206548 1103 102-103 1.9153134891925987 1103 104-105 2.050485035488645 1103 106-107 1.8260011784137333 1103 108-109 2.136931331361936 1103 110-111 2.2382634424869394 1103 112-113 2.0951042248961507 1103 114-115 1.9982962371523172 1103 116-117 2.0590560249138896 1103 118-119 2.1618420289014857 1103 120-121 2.094054676701642 1103 122-123 2.230365046351846 1103 124-125 2.166381385357841 1104 1 1.300619840247549 1104 2 1.2700726918201717 1104 3 1.275627032536427 1104 4 1.6580207482312197 1104 5 1.1844814479884747 1104 6 0.9910868092767835 1104 7 1.0427630784122712 1104 8 1.3931144731430685 1104 9 1.9579619310999483 1104 10-11 1.7642622749968666 1104 12-13 1.2654039654955938 1104 14-15 1.7059290808604892 1104 16-17 1.275719437303696 1104 18-19 0.5004345725947736 1104 20-21 -3.5685528234010064 1104 22-23 -0.3848076696409173 1104 24-25 0.8961196806455227 1104 26-27 0.9427850558860982 1104 28-29 0.6877222740388618 1104 30-31 1.244952014543383 1104 32-33 1.1867206149073581 1104 34-35 1.4687434268097839 1104 36-37 1.4246483888383246 1104 38-39 1.592249202656241 1104 40-41 1.5761718623779473 1104 42-43 1.5527066654064043 1104 44-45 1.7404888923468036 1104 46-47 1.7779807178403857 1104 48-49 1.90512023154281 1104 50-51 1.7010550454958455 1104 52-53 1.6829077868738658 1104 54-55 1.6129225985953362 1104 56-57 2.096866988911522 1104 58-59 2.0135474178527986 1104 60-61 2.1462493213343734 1104 62-63 1.7434807789486158 1104 64-65 1.9965034702532876 1104 66-67 1.8006843834810766 1104 68-69 1.898731722389737 1104 70-71 2.016776399719067 1104 72-73 1.6928644792917495 1104 74-75 1.7098995824994816 1104 76-77 1.84102672695575 1104 78-79 1.8716238675718593 1104 80-81 1.6176266647012945 1104 82-83 1.5869583537292193 1104 84-85 1.562638911869918 1104 86-87 1.4856015172699735 1104 88-89 1.5851469802748888 1104 90-91 1.6217061298107787 1104 92-93 1.2274908217073737 1104 94-95 1.3755126949241614 1104 96-97 1.579905381175319 1104 98-99 1.4665609378313107 1104 100-101 1.0607515955513733 1104 102-103 1.4965103267789246 1104 104-105 1.523089417614269 1104 106-107 1.596566295109156 1104 108-109 1.6600233887592495 1104 110-111 1.73361562383624 1104 112-113 1.7067331041096203 1104 114-115 1.622127283049597 1104 116-117 1.7211492479322388 1104 118-119 1.726720552905281 1104 120-121 1.7447651912890443 1104 122-123 1.4577625688357578 1104 124-125 1.62020926960329 1105 1 1.2359435090536905 1105 2 0.8400716996733948 1105 3 0.9362109490739883 1105 4 1.5694519962755429 1105 5 1.0751266486692401 1105 6 0.992743312798904 1105 7 0.9332556411273956 1105 8 1.1036361897096292 1105 9 1.5015545708972446 1105 10-11 1.2755882982436262 1105 12-13 1.6195304374979855 1105 14-15 1.7529265012788677 1105 16-17 1.4884233997852796 1105 18-19 1.1786724054409383 1105 20-21 1.3304599905562071 1105 22-23 1.2256277110763243 1105 24-25 1.2254711982484885 1105 26-27 1.1531770301818582 1105 28-29 0.6854821210956175 1105 30-31 1.1291861344710306 1105 32-33 1.0810162479351675 1105 34-35 0.99889848790313 1105 36-37 0.9838454940593024 1105 38-39 0.9421428330739339 1105 40-41 1.0283200509468884 1105 42-43 1.3714318140376207 1105 44-45 1.47239776366537 1105 46-47 1.7861395033000917 1105 48-49 1.3472040450497431 1105 50-51 1.4296237211325362 1105 52-53 1.4092039693983445 1105 54-55 1.3384154284269023 1105 56-57 1.6113246662195948 1105 58-59 1.4612883759613098 1105 60-61 1.6819101292471252 1105 62-63 1.6707794113124415 1105 64-65 1.453779245081762 1105 66-67 1.6935300512340206 1105 68-69 1.1692165959673382 1105 70-71 1.272386417348752 1105 72-73 1.0406573343085768 1105 74-75 1.3790438177451136 1105 76-77 1.344619824666946 1105 78-79 1.2398689698011331 1105 80-81 1.220194416878293 1105 82-83 1.3068373499819579 1105 84-85 1.286072083152611 1105 86-87 1.3121714941453213 1105 88-89 1.3752794127898333 1105 90-91 1.357694250761103 1105 92-93 1.4382993068518815 1105 94-95 1.3576854391258273 1105 96-97 1.502340395561447 1105 98-99 1.278235452633382 1105 100-101 1.4641190752696005 1105 102-103 1.4161368979959832 1105 104-105 1.465502547532072 1105 106-107 1.4649434291787173 1105 108-109 1.5987966755888792 1105 110-111 1.497102428284112 1105 112-113 1.5906432499551961 1105 114-115 1.4613565758015845 1105 116-117 1.6000305452023085 1105 118-119 1.7389624039458944 1105 120-121 1.6087708694213667 1105 122-123 1.7057644424667906 1105 124-125 1.5412127306691623 1106 1 -0.4953212788390218 1106 2 -0.2647352581659739 1106 3 -0.3310796295246341 1106 4 -0.7047869512301546 1106 5 -1.0662300803265161 1106 6 -0.6078547235552456 1106 7 -0.3738869498666624 1106 8 -0.17253022958651343 1106 9 0.14417661564750262 1106 10-11 -2.4837257453639694 1106 12-13 -0.5416323643923846 1106 14-15 -0.3590267736377548 1106 16-17 -0.47584928429875006 1106 18-19 -3.27260905667411 1106 20-21 -5.433570265239403 1106 22-23 -6.531767399681495 1106 24-25 -5.941379702532124 1106 26-27 -5.218960095890161 1106 28-29 -1.8465078557405619 1106 30-31 -3.832214360477021 1106 32-33 -4.444083968177662 1106 34-35 -3.83416095243917 1106 36-37 -6.15587758033195 1106 38-39 -6.685933126237238 1106 40-41 -2.9697517009953316 1106 42-43 -1.7397253987279413 1106 44-45 -1.0915554087839752 1106 46-47 -0.9165264316052344 1106 48-49 -0.6786332368967649 1106 50-51 -0.7053924562787053 1106 52-53 -0.9813419483669996 1106 54-55 -0.8352138387146333 1106 56-57 -0.7300218160473548 1106 58-59 -0.6200225222110483 1106 60-61 -0.413412394304828 1106 62-63 -0.587356258482064 1106 64-65 -0.7876239823273075 1106 66-67 -1.211805699913306 1106 68-69 -1.1492133101974638 1106 70-71 -0.8464352709395477 1106 72-73 -0.9011668628757477 1106 74-75 -1.0413344418014248 1106 76-77 -1.0967704390776447 1106 78-79 -1.099644357156457 1106 80-81 -1.3529027966324882 1106 82-83 -1.4091008156672835 1106 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24-25 -3.7240938158221404 2216 26-27 -3.532948938644143 2216 28-29 -3.9416277820286965 2216 30-31 -3.6466485916289564 2216 32-33 -4.0088149528971755 2216 34-35 -3.447902380131765 2216 36-37 -3.141018054009134 2216 38-39 -3.406273252274069 2216 40-41 -3.5197182925647112 2216 42-43 -3.6200588774848548 2216 44-45 -3.531441049420735 2216 46-47 -3.8311152498619947 2216 48-49 -3.576824915273008 2216 50-51 -3.5648645893123856 2216 52-53 -4.064788707508381 2216 54-55 -4.624331291884342 2216 56-57 -3.5100317732047372 2216 58-59 -4.406580497364256 2216 60-61 -4.0260211695400585 2216 62-63 -4.257172412335628 2216 64-65 -4.012606366347409 2216 66-67 -4.7893337306531905 2216 68-69 -4.375500033666491 2216 70-71 -6.6215729645949 2216 72-73 -6.9258214933324 2216 74-75 -4.849045011251736 2216 76-77 -4.14289093238013 2216 78-79 -4.554093974765831 2216 80-81 -4.593042492729307 2216 82-83 -5.016701766930197 2216 84-85 -4.640282267121744 2216 86-87 -4.852547086284186 2216 88-89 -5.040872172335423 2216 90-91 -5.13736252292723 2216 92-93 -4.9116191332863615 2216 94-95 -8.020768748997284 2216 96-97 -10.16990189187792 2216 98-99 -6.852967316666355 2216 100-101 -5.833290274990922 2216 102-103 -6.987599636671455 2216 104-105 -9.753988253290306 2216 106-107 -6.331170233032019 2216 108-109 -8.652972530063721 2216 110-111 -6.053071586840783 2216 112-113 -5.542865712636566 2216 114-115 -5.8454216090452995 2216 116-117 -5.4725772743062 2216 118-119 -5.8396997998721325 2216 120-121 -6.582902415192809 2216 122-123 -9.565740139827735 2216 124-125 -8.116488173614565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 119579.0 3 18244.0 4 10658.0 5 8930.0 6 9098.0 7 9795.0 8 10983.0 9 12008.0 10 12781.0 11 13234.0 12 13687.0 13 14357.0 14 15588.0 15 13254.0 16 10394.0 17 9164.0 18 8633.0 19 7940.0 20 8226.0 21 8655.0 22 10601.0 23 14489.0 24 19133.0 25 24271.0 26 29949.0 27 33724.0 28 38794.0 29 44573.0 30 49775.0 31 56529.0 32 67217.0 33 83461.0 34 104201.0 35 133078.0 36 176373.0 37 194601.0 38 60432.0 39 236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.64451185412395 19.160941551288786 13.65534071073904 22.539205883848226 2 14.980253219436523 20.49248402983356 41.11184874575766 23.41541400497226 3 18.800870980286415 26.608325801910247 28.725208971584742 25.865594246218592 4 10.88336507639678 16.66249975438932 38.47882632648401 33.97530884272989 5 13.237146455971601 39.12900600795324 33.487140188511646 14.146707347563517 6 30.7497109932284 37.22874505031461 18.108856169778186 13.912687786678802 7 28.509888454789685 33.33780299751934 20.579417467312194 17.57289108037878 8 25.73175414442278 35.869886897332634 19.96840087144789 18.429958086796695 9 26.4397178874882 16.92742795146061 20.455798354840326 36.17705580621086 10-11 24.48008679394367 27.791376037742626 27.111926103170138 20.616611065143573 12-13 25.230973236767717 25.267544281439925 27.822065613872077 21.67941686792029 14-15 22.506462022137992 26.27893517930609 25.706538689336316 25.508064109219603 16-17 22.061815239739538 29.93812244582793 25.834818663308006 22.165243651124523 18-19 21.930819748524115 28.200411269544105 28.427178468766 21.441590513165774 20-21 23.135254919750814 27.294657490681274 28.16255164543211 21.407535944135798 22-23 22.5721657671521 27.386942076048236 27.963868604610603 22.07702355218906 24-25 22.074463491771073 27.59818445161546 28.274595766858557 22.052756289754914 26-27 22.12682483482011 27.798915325497163 27.85912234554089 22.21513749414184 28-29 21.950395268025787 27.7454924952238 28.18857328999934 22.115538946751066 30-31 22.032577723825824 27.537234465816418 28.296433245463888 22.13375456489387 32-33 21.9019881130399 27.684656936046636 27.9508809570249 22.462473993888562 34-35 22.38754058075952 27.542389240688525 28.0055839396035 22.06448623894845 36-37 22.392389375736478 27.315727810811175 27.9508268884344 22.341055925017947 38-39 22.179464647624194 27.437698161548457 28.060978983658774 22.321858207168578 40-41 22.325658115338943 27.439282547420003 28.038345155169647 22.19671418207141 42-43 22.107640432772392 27.49555446211447 27.903619451797706 22.493185653315432 44-45 22.251225127936713 27.62236363587055 27.82525214448238 22.30115909171035 46-47 22.35610263522885 27.887699375866852 27.26857771324549 22.487620275658806 48-49 22.347752942538964 27.75017930541248 27.30752012656693 22.594547625481617 50-51 22.402993540522008 27.93420306296086 27.059642231271326 22.603161165245808 52-53 22.710045289444043 27.935127798588965 26.94518737855595 22.40963953341104 54-55 22.357253347709364 27.786326337661492 27.18887907610536 22.667541238523782 56-57 22.25739361050103 28.040457029390232 27.148395040157013 22.55375431995173 58-59 22.40656649719651 27.599038107587415 27.17986108030366 22.814534314912414 60-61 22.49813960688039 27.61158717529493 27.384580262456844 22.50569295536784 62-63 22.375344593059342 27.701336992287146 27.31053011373391 22.612788300919604 64-65 22.16360599192647 27.67935601385253 27.229558563235262 22.927479430985738 66-67 22.149834817201768 27.958289142363324 27.13576143242405 22.756114608010858 68-69 22.282606487231828 27.757892997381756 27.25357959909574 22.70592091629068 70-71 22.000535011055767 27.69525363926033 27.55076679274417 22.753444556939737 72-73 22.399920088444482 27.64370958921736 27.21062959131673 22.74574073102143 74-75 22.23079655390875 27.924100121255872 27.072904882368938 22.77219844246644 76-77 22.338608471510256 27.67625727287073 27.337291210789484 22.647843044829532 78-79 22.406971077236538 27.716223049273925 27.242514115252803 22.63429175823673 80-81 22.16836023780089 27.99422462276012 27.132861781366618 22.704553358072374 82-83 22.350517069734998 28.056061601392397 27.04758937822957 22.545831950643034 84-85 22.363456838281454 27.872114780976297 26.942356908103026 22.822071472639223 86-87 22.277645793602634 27.687612419816226 27.261128207350904 22.773613579230236 88-89 22.370955808279618 27.64843023816901 27.18265719205392 22.79795676149745 90-91 22.292842654891828 27.895529918494915 27.24707858282061 22.564548843792647 92-93 22.286825511523283 27.83334766788183 27.1244647400582 22.75536208053669 94-95 22.244822842104515 27.632988206404345 27.157912416070225 22.964276535420915 96-97 22.390266729693234 27.599507255053073 27.119087703995458 22.89113831125823 98-99 22.0210677728901 27.5923894879879 27.161810234282385 23.224732504839615 100-101 22.053747839233925 27.601156020431834 27.254282168537213 23.09081397179703 102-103 22.283126359719294 27.393622334526924 27.270911107160696 23.05234019859309 104-105 22.183751151733553 27.359500358429976 27.368406082033562 23.0883424078029 106-107 21.903737369017364 27.570977640257155 27.49069331659248 23.034591674133008 108-109 22.01543190124179 27.583262140169772 27.086755430404573 23.314550528183858 110-111 21.905939965440865 27.64662997651022 27.37627907905969 23.071150978989223 112-113 21.989075997331646 27.697900194031416 27.348372743495887 22.964651065141055 114-115 21.931703590630594 27.867123845230203 27.149559831314384 23.051612732824818 116-117 21.648556276419335 27.80931721418865 27.4212833798326 23.120843129559415 118-119 21.940993136815358 27.800333745548816 27.045071902932055 23.213601214703765 120-121 21.8625890607645 27.876357922683066 27.231124536071306 23.029928480481132 122-123 21.809782649183607 27.820267880665305 27.29248459080033 23.07746487935076 124-125 21.840447315604955 27.86300688563557 27.02659392096358 23.26995187779589 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1316.0 1 1218.0 2 1325.0 3 1461.0 4 1155.0 5 811.0 6 646.5 7 701.0 8 938.0 9 1040.0 10 936.5 11 853.0 12 909.5 13 964.0 14 1045.5 15 1180.5 16 1320.5 17 1484.5 18 1672.5 19 1860.5 20 2123.5 21 2608.0 22 3276.5 23 4046.5 24 4965.5 25 6339.5 26 8384.5 27 11046.5 28 13563.0 29 16049.0 30 18862.5 31 21877.0 32 25107.0 33 28291.5 34 31707.0 35 34491.0 36 37061.0 37 39882.5 38 41259.0 39 42009.5 40 43176.5 41 44745.0 42 46311.0 43 48029.0 44 50184.0 45 51926.5 46 52234.5 47 52361.0 48 54367.0 49 55220.5 50 53720.5 51 51859.0 52 50232.5 53 49126.5 54 47392.5 55 43940.0 56 40006.0 57 37070.0 58 34886.5 59 31416.0 60 26489.5 61 21658.0 62 17331.5 63 13329.5 64 10094.5 65 7861.5 66 6036.5 67 4708.0 68 3629.0 69 2715.0 70 2075.0 71 1642.0 72 1345.5 73 1159.5 74 917.0 75 659.0 76 507.5 77 343.0 78 224.5 79 150.5 80 94.5 81 72.5 82 56.5 83 41.5 84 34.0 85 27.5 86 21.5 87 15.0 88 13.0 89 13.0 90 11.0 91 8.5 92 6.0 93 4.5 94 2.0 95 0.5 96 2.0 97 3.0 98 1.5 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09616393920001085 2 0.03081309319436967 3 0.04110669795380745 4 0.04957183344676615 5 0.05200979246873825 6 0.0024379590219721055 7 0.0016253060146480703 8 0.0014898638467607313 9 0.0048081969600005416 10-11 0.06541856708958484 12-13 0.004537312624225863 14-15 0.009040764706479892 16-17 0.017709063451269602 18-19 0.016320781230424374 20-21 0.13043080767550766 22-23 0.013578077330705755 24-25 0.011952771316057686 26-27 0.004943639127887881 28-29 0.0035214963650708193 30-31 0.001523724388732566 32-33 0.004672754792113203 34-35 0.01814925049690345 36-37 0.00169302709859174 38-39 0.007009132188169804 40-41 0.028578297424228575 42-43 0.011140118308733649 44-45 0.11492267945240732 46-47 0.0025056801059157754 48-49 0.007720203569578335 50-51 0.008973043622536222 52-53 0.009616393920001083 54-55 0.007652482485634665 56-57 0.004469591540282193 58-59 0.012223655651832362 60-61 0.03240453866704591 62-63 0.005722431593240081 64-65 0.0061964791808457684 66-67 0.00670438731042329 68-69 0.005620849967324577 70-71 0.0027088433577467843 72-73 8.4651354929587E-4 74-75 0.0010496768011268788 76-77 0.006501224058592281 78-79 0.02143372306817143 80-81 0.0021332141442255924 82-83 0.003724659616901828 84-85 0.0016253060146480703 86-87 0.0025056801059157754 88-89 0.004571173166197698 90-91 0.00338605419718348 92-93 0.0017607481825354097 94-95 0.0026072617318312796 96-97 0.0012867005949297225 98-99 0.0019977719763382535 100-101 0.001862329808450914 102-103 5.079081295775221E-4 104-105 0.004503452082254028 106-107 0.0012189795109860528 108-109 0.0019300508923945838 110-111 0.0030813093194369664 112-113 0.005417686715493569 114-115 0.0035214963650708193 116-117 0.0066705267684514565 118-119 0.0048081969600005416 120-121 0.0084651354929587 122-123 0.006230339722817603 124-125 0.055463567749865404 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1476645.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.27577531261221 #Duplication Level Percentage of deduplicated Percentage of total 1 84.64588648209714 57.79263526588544 2 9.12880364245743 12.465522927307589 3 2.729935903726418 5.591654711419736 4 1.1174543651426856 3.0518025262631885 5 0.5924147402896836 2.0223787849948973 6 0.395560677238666 1.6204327152991127 7 0.23847420189413907 1.1397407718465133 8 0.18047759234290284 0.9857798035012204 9 0.14428821029798722 0.8866250483907884 >10 0.7550595055727907 9.422217948570994 >50 0.0515642904709316 2.4216907051638694 >100 0.019668500327700376 2.1743847253236956 >500 1.0297203539424028E-4 0.06566482845623574 >1k 3.089161061827209E-4 0.3594692375768023 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2086 0.1412661811064948 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1610 0.10903094514930806 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.354421678873392E-4 0.0 0.0 0.0 0.0 3 1.354421678873392E-4 0.0 0.0 0.0 0.0 4 1.354421678873392E-4 0.0 0.0 0.0 0.0 5 2.031632518310088E-4 0.0 0.0 0.0 0.0 6 2.031632518310088E-4 0.0 0.0 0.0 0.0 7 2.708843357746784E-4 0.0 0.0 0.0 0.0 8 4.063265036620176E-4 0.0 0.0 0.0 0.0 9 4.063265036620176E-4 0.0 0.0 0.0 0.0 10-11 4.401870456338524E-4 0.0 0.0 3.38605419718348E-5 0.0 12-13 7.110713814085308E-4 0.0 0.0 1.354421678873392E-4 0.0 14-15 8.803740912677048E-4 0.0 0.0 1.354421678873392E-4 0.0 16-17 9.480951752113744E-4 0.0 0.0 1.354421678873392E-4 0.0 18-19 0.0010835373430987138 0.0 0.0 1.6930270985917402E-4 0.0 20-21 0.0011512584270423833 0.0 0.0 2.708843357746784E-4 6.77210839436696E-5 22-23 0.0011512584270423833 0.0 0.0 3.7246596169018283E-4 6.77210839436696E-5 24-25 0.0014221427628170616 0.0 0.0 5.417686715493568E-4 1.015816259155044E-4 26-27 0.0014221427628170616 0.0 0.0 7.110713814085308E-4 2.370237938028436E-4 28-29 0.001862329808450914 0.0 0.0 0.0014221427628170616 2.708843357746784E-4 30-31 0.0018961903504227488 0.0 0.0 0.004198707204507515 2.708843357746784E-4 32-33 0.0018961903504227488 0.0 0.0 0.009548672836057414 2.708843357746784E-4 34-35 0.0021332141442255924 0.0 0.0 0.01659166556619905 2.708843357746784E-4 36-37 0.0025056801059157754 0.0 0.0 0.029390950431552605 2.708843357746784E-4 38-39 0.0026072617318312796 0.0 0.0 0.053736680109301826 2.708843357746784E-4 40-41 0.0026749828157749496 0.0 0.0 0.08289060674705159 2.708843357746784E-4 42-43 0.0028104249836622885 0.0 0.0 0.11001290086649126 2.708843357746784E-4 44-45 0.003250612029296141 0.0 0.0 0.13974245671776223 2.708843357746784E-4 46-47 0.003758520158873663 0.0 0.0 0.17553304958199162 2.708843357746784E-4 48-49 0.003860101784789167 0.0 0.0 0.21904384601579935 2.708843357746784E-4 50-51 0.003860101784789167 0.0 0.0 0.2714261044462277 2.708843357746784E-4 52-53 0.003860101784789167 0.0 0.0 0.32103179843496576 3.3860541971834804E-4 54-55 0.003860101784789167 0.0 0.0 0.3724998222321546 3.3860541971834804E-4 56-57 0.0040632650366201764 0.0 0.0 0.4387649028710353 3.3860541971834804E-4 58-59 0.0040632650366201764 0.0 0.0 0.5149849828496356 3.3860541971834804E-4 60-61 0.004097125578592011 0.0 0.0 0.6021420178851382 4.063265036620176E-4 62-63 0.004164846662535681 0.0 0.0 0.6931591547054302 4.063265036620176E-4 64-65 0.004266428288451185 0.0 0.0 0.78173833250375 4.063265036620176E-4 66-67 0.004605033708169533 0.0 0.0 0.8862996861127759 4.063265036620176E-4 68-69 0.004672754792113203 0.0 0.0 1.0108048989432126 4.063265036620176E-4 70-71 0.0048081969600005416 0.0 0.0 1.1516308930040733 4.063265036620176E-4 72-73 0.004875918043944211 0.0 0.0 1.3014299306874706 4.401870456338524E-4 74-75 0.004875918043944211 0.0 0.0 1.4500777099438253 4.740475876056872E-4 76-77 0.004909778585916047 0.0 0.0 1.6119988216531393 4.740475876056872E-4 78-79 0.004943639127887881 0.0 0.0 1.803920373549499 4.740475876056872E-4 80-81 0.005586989425352742 3.38605419718348E-5 0.0 2.026925902976003 4.740475876056872E-4 82-83 0.0056885710512682465 6.77210839436696E-5 0.0 2.2607329452915224 5.417686715493568E-4 84-85 0.005756292135211916 6.77210839436696E-5 0.0 2.488275787342252 5.417686715493568E-4 86-87 0.0058240132191555855 6.77210839436696E-5 0.0 2.7200511971394614 5.417686715493568E-4 88-89 0.005857873761127421 6.77210839436696E-5 0.0 2.9910032539980835 5.417686715493568E-4 90-91 0.006501224058592282 6.77210839436696E-5 0.0 3.2957481317445967 5.417686715493568E-4 92-93 0.006873690020282465 6.77210839436696E-5 0.0 3.632660524364353 5.417686715493568E-4 94-95 0.0069075505622543 6.77210839436696E-5 0.0 3.966153002244954 5.756292135211916E-4 96-97 0.0069075505622543 6.77210839436696E-5 0.0 4.294972725333442 6.094897554930264E-4 98-99 0.007347737607888151 6.77210839436696E-5 0.0 4.65572293950137 6.094897554930264E-4 100-101 0.00775406411155017 6.77210839436696E-5 0.0 5.0693294596873315 6.094897554930264E-4 102-103 0.008126530073240353 6.77210839436696E-5 0.0 5.512631675182593 6.094897554930264E-4 104-105 0.009243927958310902 6.77210839436696E-5 0.0 5.9596585502947566 6.094897554930264E-4 106-107 0.009616393920001083 6.77210839436696E-5 0.0 6.390635528512269 6.094897554930264E-4 108-109 0.009751836087888422 6.77210839436696E-5 0.0 6.8450778623162645 6.094897554930264E-4 110-111 0.009819557171832092 6.77210839436696E-5 0.0 7.370864358054915 6.094897554930264E-4 112-113 0.009954999339719432 6.77210839436696E-5 0.0 7.9496426019794875 6.094897554930264E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3050 0.0 53.27936 1 GTATCAA 5345 0.0 48.666473 1 ATCAACG 6445 0.0 38.587223 3 TCAACGC 6535 0.0 38.5672 4 TATCAAC 6835 0.0 37.9523 2 CAACGCA 6690 0.0 37.762703 5 AACGCAG 6680 0.0 37.44212 6 GTACATG 8295 0.0 35.87978 1 TACATGG 8565 0.0 33.20402 2 ACGCAGA 7625 0.0 31.787539 7 CGCAGAG 7475 0.0 31.31323 8 ACATGGG 8750 0.0 29.64617 3 GCAGAGT 7895 0.0 27.157944 9 GAGTACT 3085 0.0 26.319601 12-13 CATGGGA 5235 0.0 26.177881 4 TACCTGG 1320 0.0 23.888674 2 GTACTTT 3565 0.0 23.6478 14-15 CAGAGTA 8065 0.0 23.431427 10-11 ATGGGAG 2395 0.0 23.385445 5 ACGCATA 230 2.3421671E-7 23.30565 8 >>END_MODULE