FastQCFastQC Report
Thu 26 May 2016
SRR1364501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476645
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53100.35959895574088563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50200.3399598413972214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32100.21738467945917944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38550.067.1851041
GTATCAA66250.048.9126971
TATCAAC88950.036.194492
ATCAACG86350.036.0414923
TCAACGC87100.035.52584
CAACGCA87800.035.2410775
AACGCAG89350.034.0969666
GTACATG80000.032.7478071
TACATGG83700.030.7718012
CGCAGAG106900.028.9268518
ACATGGG83150.028.609173
ACGCAGA105650.028.554787
TGGTATC17150.026.7683282
GCAGAGT114050.026.6441069
GAGTACT68050.026.17332812-13
CATGGGA52650.025.3655514
AACGCCG2151.2318742E-724.9083187
CAGAGTA114450.024.13571510-11
AGTACTT70700.023.80440112-13
GTACTTT76550.023.69435514-15
Produced by FastQC (version 0.11.5)