##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364501_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1476645 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.943519938780142 31.0 28.0 31.0 25.0 34.0 2 29.109255779148 31.0 28.0 33.0 25.0 34.0 3 29.225098110920364 31.0 28.0 34.0 25.0 34.0 4 32.64790657199259 35.0 32.0 37.0 26.0 37.0 5 31.75632531854305 35.0 32.0 37.0 20.0 37.0 6 31.80646600909494 35.0 32.0 37.0 21.0 37.0 7 31.23174696694195 35.0 31.0 37.0 17.0 37.0 8 31.369094128920626 35.0 32.0 37.0 17.0 37.0 9 31.91103413481236 37.0 30.0 39.0 11.0 39.0 10-11 32.12286500817732 37.0 31.0 39.0 15.0 39.0 12-13 31.854853400783533 37.0 30.5 39.0 11.5 39.0 14-15 32.81316226987529 38.0 31.0 40.0 12.0 41.0 16-17 32.89131815703842 38.0 31.0 40.0 12.0 41.0 18-19 32.77265084024935 38.0 31.0 40.0 10.5 41.0 20-21 32.6567675372212 38.0 31.0 40.0 10.5 41.0 22-23 32.635163157021495 38.0 31.0 40.0 10.0 41.0 24-25 32.42553795936058 38.0 30.5 40.0 10.0 41.0 26-27 32.52625885029916 37.5 31.0 40.0 10.0 41.0 28-29 32.40311009078011 37.0 30.5 40.0 9.0 41.0 30-31 31.83618642259988 37.0 30.0 40.0 8.0 41.0 32-33 31.753190509567297 37.0 30.0 40.0 7.5 41.0 34-35 31.513384056425206 37.0 30.0 40.0 2.0 41.0 36-37 31.47987329385194 36.5 30.0 40.0 2.0 41.0 38-39 31.22947831062984 36.0 30.0 40.0 2.0 41.0 40-41 30.924255999241524 36.0 29.5 40.0 2.0 41.0 42-43 30.78605521299974 36.0 28.5 40.0 2.0 41.0 44-45 30.678534786627793 36.0 28.5 40.0 2.0 41.0 46-47 30.478031618974093 36.0 28.0 40.0 2.0 41.0 48-49 30.142686630842213 36.0 27.5 40.0 2.0 41.0 50-51 29.784679459179422 35.0 27.0 40.0 2.0 41.0 52-53 29.69589847255095 35.0 27.0 40.0 2.0 41.0 54-55 29.21969261399998 35.0 26.0 39.5 2.0 41.0 56-57 29.247688171496875 35.0 26.0 39.0 2.0 41.0 58-59 29.00968716245272 35.0 26.0 39.0 2.0 41.0 60-61 28.729137334972187 34.5 25.5 39.0 2.0 41.0 62-63 28.455667069607117 34.0 24.5 39.0 2.0 40.5 64-65 27.961979690446924 34.0 23.0 38.0 2.0 40.0 66-67 27.574134947803977 34.0 22.0 38.0 2.0 40.0 68-69 27.380528833944517 34.0 22.0 37.5 2.0 40.0 70-71 27.466294877915814 34.0 23.5 37.0 2.0 40.0 72-73 27.240876107662977 34.0 22.5 36.5 2.0 39.0 74-75 26.869914569852604 33.0 21.5 36.0 2.0 39.0 76-77 26.802061768400666 33.0 22.0 36.0 2.0 39.0 78-79 26.39603425332426 33.0 21.0 35.0 2.0 37.5 80-81 26.172845199760268 33.0 20.0 35.0 2.0 37.0 82-83 26.039199333624534 33.0 20.0 35.0 2.0 37.0 84-85 25.59590084278889 33.0 18.5 35.0 2.0 36.0 86-87 25.471710194393374 33.0 18.5 35.0 2.0 36.0 88-89 25.434183910147667 33.0 19.0 35.0 2.0 36.0 90-91 25.27438043673327 33.0 18.5 35.0 2.0 35.0 92-93 25.297659559338904 33.0 19.0 35.0 2.0 35.0 94-95 25.316259493649454 33.0 19.0 35.0 2.0 35.0 96-97 25.193407691083504 33.0 18.0 35.0 2.0 35.0 98-99 25.05348712791497 33.0 17.5 35.0 2.0 35.0 100-101 24.95671132872153 33.0 16.5 35.0 2.0 35.0 102-103 23.84353111275899 30.5 13.0 34.0 2.0 35.0 104-105 24.78099373918579 32.0 17.0 35.0 2.0 35.0 106-107 24.87025927016988 33.0 15.0 35.0 2.0 35.0 108-109 24.943145102580512 33.0 15.5 35.0 2.0 35.0 110-111 24.86811691367932 33.0 10.5 35.0 2.0 35.0 112-113 24.82254299442317 33.0 9.0 35.0 2.0 35.0 114-115 24.76695177243007 33.0 7.0 35.0 2.0 35.0 116-117 24.688201971360755 33.0 2.0 35.0 2.0 35.0 118-119 24.572221488577146 33.0 2.0 35.0 2.0 35.0 120-121 24.41190164189768 33.0 2.0 35.0 2.0 35.0 122-123 24.13527184936122 33.0 2.0 35.0 2.0 35.0 124-125 23.363578585238834 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9100177315387228 1101 2 1.0036791911556229 1101 3 1.0150485813704364 1101 4 1.0670690602691195 1101 5 1.2655729337672668 1101 6 1.289034971086373 1101 7 1.8141315958986866 1101 8 1.646931020191495 1101 9 2.335158589780402 1101 10-11 1.9209076341881328 1101 12-13 2.2252107727932895 1101 14-15 2.1432111026343605 1101 16-17 1.9995312748123482 1101 18-19 2.0891823268472933 1101 20-21 2.3455551681612405 1101 22-23 2.3460433333285096 1101 24-25 2.420911512319087 1101 26-27 2.2462344014996134 1101 28-29 2.1540804527400894 1101 30-31 2.790120185358081 1101 32-33 2.534692720344758 1101 34-35 2.5879066696394197 1101 36-37 2.486782009723484 1101 38-39 2.6457381087039344 1101 40-41 2.817944728500816 1101 42-43 2.634163891714934 1101 44-45 2.546508081263493 1101 46-47 2.32539078803946 1101 48-49 2.3009836808782467 1101 50-51 2.4059700653587406 1101 52-53 2.246874304626143 1101 54-55 2.6044554204999955 1101 56-57 2.2566580861588577 1101 58-59 2.381268233755815 1101 60-61 2.426141509599489 1101 62-63 2.4536805099133083 1101 64-65 2.8106006562688393 1101 66-67 2.9112722268247673 1101 68-69 2.788206392442813 1101 70-71 2.431034622597089 1101 72-73 2.571994035580502 1101 74-75 2.815492757893974 1101 76-77 2.4784557354300176 1101 78-79 2.5258881486711147 1101 80-81 2.5174914254550984 1101 82-83 2.3677022551039 1101 84-85 2.7026682027410374 1101 86-87 2.5615687398636204 1101 88-89 2.4055566157006467 1101 90-91 2.4663436705595743 1101 92-93 2.469609975254251 1101 94-95 2.273058658188642 1101 96-97 2.224267068710205 1101 98-99 2.396067195963127 1101 100-101 2.4853109479032796 1101 102-103 2.203742907383962 1101 104-105 2.2148191506746038 1101 106-107 2.355878454162891 1101 108-109 2.43079912303628 1101 110-111 2.354540576221602 1101 112-113 2.274218472835848 1101 114-115 2.276379051573219 1101 116-117 2.233165468257731 1101 118-119 2.17171989506981 1101 120-121 2.2003241789763237 1101 122-123 2.3355044864821366 1101 124-125 2.130670851338113 1102 1 0.45851213901152477 1102 2 -1.8767950231652932 1102 3 -0.17460369301099377 1102 4 0.33193527696608527 1102 5 0.9038627334023701 1102 6 0.34229289968087784 1102 7 1.1692068918440697 1102 8 0.997757252032514 1102 9 1.4695607915422038 1102 10-11 1.3375349992217025 1102 12-13 1.6335556246329332 1102 14-15 1.6343784345729588 1102 16-17 1.5202855969040527 1102 18-19 1.6322367899353125 1102 20-21 1.762885678848562 1102 22-23 1.5631664293071807 1102 24-25 1.9508077478482022 1102 26-27 1.3963556925949732 1102 28-29 1.3783837123005895 1102 30-31 1.666566458257737 1102 32-33 1.5156778014992298 1102 34-35 1.7021605968109021 1102 36-37 1.199014092258551 1102 38-39 1.1096328951043155 1102 40-41 1.1941023587836916 1102 42-43 1.2354546035831078 1102 44-45 1.2593174490321282 1102 46-47 0.985050544690278 1102 48-49 0.8804723897502456 1102 50-51 1.102380248751853 1102 52-53 0.9499422390628602 1102 54-55 1.2287673699371524 1102 56-57 0.9643477702553227 1102 58-59 1.0232198168636693 1102 60-61 0.8849541460672725 1102 62-63 1.2350105750914615 1102 64-65 1.2613376230857511 1102 66-67 1.411632061154105 1102 68-69 1.3173790835466654 1102 70-71 0.7071009887762543 1102 72-73 0.991375642742593 1102 74-75 1.30780996858228 1102 76-77 1.177600170155582 1102 78-79 1.058443160078113 1102 80-81 1.182668029859272 1102 82-83 0.7945308417790962 1102 84-85 0.9282004882846735 1102 86-87 0.8298333918932208 1102 88-89 0.9204287480413491 1102 90-91 0.7068927157338756 1102 92-93 0.7679438443953188 1102 94-95 0.8099220941250742 1102 96-97 0.9774431574334379 1102 98-99 0.7054006946625044 1102 100-101 0.6594897341600578 1102 102-103 0.46315082080178627 1102 104-105 0.6248632201805613 1102 106-107 0.8080082490981226 1102 108-109 0.6996751262478611 1102 110-111 0.6258075194412136 1102 112-113 0.6592371254725116 1102 114-115 0.9082193716217866 1102 116-117 0.59243495561212 1102 118-119 0.19362226002177962 1102 120-121 0.49887978276364464 1102 122-123 0.8155440845773825 1102 124-125 0.8660867496510534 1103 1 0.827354870927369 1103 2 0.7792740614973468 1103 3 0.7220020648091392 1103 4 0.5155444703336443 1103 5 0.9036709590470267 1103 6 0.9389471747738156 1103 7 1.3616639311102787 1103 8 1.116241021693483 1103 9 1.7094074048398689 1103 10-11 1.5260762356253892 1103 12-13 1.4511163367878197 1103 14-15 1.35535494303398 1103 16-17 1.3813922004982615 1103 18-19 1.5555590101906205 1103 20-21 1.802250643476846 1103 22-23 1.4977275282889693 1103 24-25 1.8121987347813189 1103 26-27 1.4811998636380252 1103 28-29 1.469287215378813 1103 30-31 2.0110095914245427 1103 32-33 1.8587940856006497 1103 34-35 1.9105521180896652 1103 36-37 1.7791003743274842 1103 38-39 1.9513252136398798 1103 40-41 2.1343724328053106 1103 42-43 2.0653380473915774 1103 44-45 1.9202366218781854 1103 46-47 1.884217801431877 1103 48-49 1.8377673550997358 1103 50-51 2.040385910193077 1103 52-53 1.88596623216554 1103 54-55 2.1691169401658996 1103 56-57 1.8110815415553922 1103 58-59 1.8737124474833173 1103 60-61 1.9964385615324822 1103 62-63 2.234279537805282 1103 64-65 2.1969555298932484 1103 66-67 2.4329042627237065 1103 68-69 2.3979800420906194 1103 70-71 2.2448764390362115 1103 72-73 2.3682171682829996 1103 74-75 2.3635970465967446 1103 76-77 2.2211546668544386 1103 78-79 2.3056276011132333 1103 80-81 2.305947797500874 1103 82-83 2.160853871748344 1103 84-85 2.443753470457853 1103 86-87 2.3064983866808895 1103 88-89 2.1478022425307053 1103 90-91 2.1703084792580647 1103 92-93 2.111249853980759 1103 94-95 1.9179254772213952 1103 96-97 1.92354882674233 1103 98-99 2.043623454508559 1103 100-101 2.0278640940926564 1103 102-103 1.989931208022071 1103 104-105 1.8801775826045883 1103 106-107 1.8804817448987556 1103 108-109 1.8740615302656138 1103 110-111 1.8914469639840057 1103 112-113 1.7702138306251776 1103 114-115 1.6410322096855516 1103 116-117 1.78115362427193 1103 118-119 1.8260805613927218 1103 120-121 1.7885737961255614 1103 122-123 1.9828528675150352 1103 124-125 1.7007457027269837 1104 1 0.6596823799647389 1104 2 0.6788921872853777 1104 3 0.6617998333686081 1104 4 0.6115933578512127 1104 5 0.973234536817948 1104 6 0.9185368076031892 1104 7 1.1899534774703753 1104 8 1.0376602605353256 1104 9 1.5803653472521013 1104 10-11 1.152686106835695 1104 12-13 1.1251420337796745 1104 14-15 1.2226166097197009 1104 16-17 1.0824582104555631 1104 18-19 1.0387086439342 1104 20-21 1.3181012801878182 1104 22-23 1.3192299033370603 1104 24-25 1.3858247494620528 1104 26-27 1.3122230960651464 1104 28-29 1.3407806387620838 1104 30-31 1.6575216799812615 1104 32-33 1.4799069679986232 1104 34-35 1.523474036717122 1104 36-37 1.3408127706691033 1104 38-39 1.4295114349326532 1104 40-41 1.5977137464526017 1104 42-43 1.5472131164336744 1104 44-45 1.34319040462319 1104 46-47 1.4508107597095083 1104 48-49 1.2972152659335343 1104 50-51 1.7276918780965964 1104 52-53 1.5527689831835545 1104 54-55 1.7966741641594126 1104 56-57 1.7592067391586284 1104 58-59 1.6857412946993477 1104 60-61 1.8226189432924151 1104 62-63 1.9028058652228985 1104 64-65 1.9714924240845697 1104 66-67 1.9859040227429041 1104 68-69 2.081175481693549 1104 70-71 1.8997668097113234 1104 72-73 2.048954709279723 1104 74-75 2.1065199937133556 1104 76-77 1.9815303129932254 1104 78-79 1.995235194410533 1104 80-81 1.968156439857104 1104 82-83 1.9572654921520467 1104 84-85 2.243643955409535 1104 86-87 2.095542120325053 1104 88-89 1.8895184290453066 1104 90-91 2.040386396834183 1104 92-93 1.766743795417824 1104 94-95 1.6689919633310772 1104 96-97 1.6758684506962211 1104 98-99 1.9102213647709725 1104 100-101 1.9254021005759476 1104 102-103 1.7844652643451866 1104 104-105 1.5897125340941827 1104 106-107 1.7608413161330567 1104 108-109 1.6581724204420212 1104 110-111 1.7555541211257193 1104 112-113 1.721780181507583 1104 114-115 1.6323300487155699 1104 116-117 1.8070110794850365 1104 118-119 1.7766516585620913 1104 120-121 1.6339071218404584 1104 122-123 1.7202394004019297 1104 124-125 1.4696599991070798 1105 1 0.19488031324668498 1105 2 0.3099816789246326 1105 3 0.16934129365602502 1105 4 0.3660969410274575 1105 5 0.5616369133910766 1105 6 0.6849719097561504 1105 7 0.8791159911123927 1105 8 0.8777407007432174 1105 9 1.1739018925970193 1105 10-11 0.800205930691483 1105 12-13 1.0057740885156576 1105 14-15 0.8651630116612452 1105 16-17 0.9938708557478222 1105 18-19 1.187123689458474 1105 20-21 1.2909612959095256 1105 22-23 1.2093506018083957 1105 24-25 1.321448256471946 1105 26-27 1.1172866697778332 1105 28-29 1.1992414565962974 1105 30-31 1.5081272711099452 1105 32-33 1.393034882429781 1105 34-35 1.46942779504543 1105 36-37 1.2409645500461863 1105 38-39 1.3989183982766455 1105 40-41 1.7089435123822803 1105 42-43 1.5319837588105543 1105 44-45 1.4385853171997915 1105 46-47 1.31822980434848 1105 48-49 1.5262025511909982 1105 50-51 1.5825589304285224 1105 52-53 1.6262668348041878 1105 54-55 1.894699792073844 1105 56-57 1.5658919004588014 1105 58-59 1.5679329278492595 1105 60-61 1.5510121806673496 1105 62-63 1.4294570264162196 1105 64-65 1.96985595429544 1105 66-67 2.056407403673841 1105 68-69 2.038539118750993 1105 70-71 1.9219845057938656 1105 72-73 1.9987465492540828 1105 74-75 2.0595036843467263 1105 76-77 1.931467063636216 1105 78-79 1.9743107234918789 1105 80-81 1.8996056801016294 1105 82-83 1.8355092397193786 1105 84-85 2.1625153502915815 1105 86-87 2.011966537820417 1105 88-89 1.789955164424498 1105 90-91 1.9308441390133986 1105 92-93 1.7285918405070788 1105 94-95 1.6427499672843986 1105 96-97 1.7374116968478361 1105 98-99 1.7538501606863832 1105 100-101 1.680237957523648 1105 102-103 1.5442045205403794 1105 104-105 1.1048571776463767 1105 106-107 1.3497920445977485 1105 108-109 1.3362084849773552 1105 110-111 1.5531205949597542 1105 112-113 1.5956780399969261 1105 114-115 1.4335109895759928 1105 116-117 1.3886742990776497 1105 118-119 1.4632949779799311 1105 120-121 1.506026557232623 1105 122-123 1.6566443183971842 1105 124-125 1.4716168178266003 1106 1 0.16164520360668888 1106 2 0.23575732524481552 1106 3 0.27799853747227843 1106 4 0.0817564267037767 1106 5 0.18562621332214135 1106 6 0.30230394103040936 1106 7 0.6608629962730213 1106 8 0.3394086197878501 1106 9 1.0849649441349634 1106 10-11 0.5894509020316931 1106 12-13 0.5541522331746265 1106 14-15 0.5974854637966089 1106 16-17 0.5280075998963198 1106 18-19 0.5403020384310082 1106 20-21 0.39610748257387485 1106 22-23 0.30584296023275215 1106 24-25 0.15737367581156292 1106 26-27 0.30053156038623996 1106 28-29 0.31518668627828106 1106 30-31 0.8814187067077093 1106 32-33 0.5411129221374082 1106 34-35 0.5724198398577975 1106 36-37 0.5083090792245315 1106 38-39 0.7412189130971392 1106 40-41 0.978108000325296 1106 42-43 1.029102642600229 1106 44-45 0.9065798150169293 1106 46-47 0.8476596515169668 1106 48-49 0.8719349438953259 1106 50-51 0.9692262284592879 1106 52-53 0.7097442951058746 1106 54-55 1.2075883138745347 1106 56-57 0.8397365041588003 1106 58-59 0.7654390137851586 1106 60-61 1.0596594106349926 1106 62-63 1.2064835499184916 1106 64-65 1.1593047734375688 1106 66-67 1.1766554831884584 1106 68-69 1.1466832498594393 1106 70-71 1.132642112903234 1106 72-73 0.8229460623792058 1106 74-75 1.1006070396846503 1106 76-77 0.6729145382833188 1106 78-79 0.722017241653429 1106 80-81 0.9107004809735351 1106 82-83 0.7881212240449891 1106 84-85 1.031822981930997 1106 86-87 1.0071311685966258 1106 88-89 0.9445208583764462 1106 90-91 1.0307190823458825 1106 92-93 1.0122496277104673 1106 94-95 0.8577298309097579 1106 96-97 0.7866107442125667 1106 98-99 0.7473112727980151 1106 100-101 0.5614981654390903 1106 102-103 0.5054190994619248 1106 104-105 0.7053756465987853 1106 106-107 0.5351227655327477 1106 108-109 0.580739293475304 1106 110-111 0.5256481058740725 1106 112-113 0.26331491145326424 1106 114-115 0.46315702719701335 1106 116-117 0.7098207527779579 1106 118-119 0.4476504742631633 1106 120-121 0.3946255965241683 1106 122-123 0.5788863958127735 1106 124-125 0.5601462259382828 1107 1 0.3909953255653349 1107 2 0.4845458359149468 1107 3 0.43749831807780737 1107 4 0.4910645745286075 1107 5 0.5042650653453933 1107 6 0.8238747745983019 1107 7 0.9273038935617066 1107 8 0.9294937633504929 1107 9 1.5197660354778684 1107 10-11 0.9320738304119303 1107 12-13 1.3181965639675042 1107 14-15 1.1831565949544043 1107 16-17 1.21326271055365 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-4.549363233203856 2216 108-109 -4.614583904705842 2216 110-111 -4.349579147147825 2216 112-113 -4.318115987801221 2216 114-115 -4.1739814839499125 2216 116-117 -4.009867031503358 2216 118-119 -4.29499261772666 2216 120-121 -4.127296664707053 2216 122-123 -4.154055865060627 2216 124-125 -4.100559163312937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75583.0 3 24859.0 4 11694.0 5 9931.0 6 10284.0 7 12247.0 8 13411.0 9 13909.0 10 15136.0 11 16411.0 12 18148.0 13 19820.0 14 21325.0 15 16960.0 16 11691.0 17 9933.0 18 8728.0 19 7829.0 20 7833.0 21 8459.0 22 10187.0 23 13693.0 24 18899.0 25 24761.0 26 30902.0 27 34822.0 28 39072.0 29 44337.0 30 48842.0 31 55281.0 32 64964.0 33 77447.0 34 94755.0 35 120133.0 36 168113.0 37 216713.0 38 79100.0 39 433.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6263148047215 20.064550505584617 13.992383013224652 23.316751676469234 2 15.70776608229906 20.950144212634232 39.00648702898948 24.335602676077226 3 18.935424560405515 26.124830274033368 29.61334647122362 25.326398694337506 4 12.585557134764572 17.40314718642619 36.57120178559919 33.44009389321004 5 12.764069901702847 39.233668214093434 33.62832637499196 14.37393550921176 6 30.921712913488847 37.572817243448306 17.199429516429845 14.306040326632996 7 27.41499608083307 33.329652410202975 20.74123403964151 18.514117469322443 8 26.170203400275625 36.039874174226036 19.093756454665815 18.696165970832528 9 26.083655854995612 15.989421966687999 20.135509888971285 37.791412289345104 10-11 25.02068897328515 27.39602690446909 26.636189653210817 20.947094469034937 12-13 25.893037028509124 24.754365084067974 27.67089983458985 21.681698052833053 14-15 22.709997589114717 25.633162133389675 26.27864957917862 25.37819069831699 16-17 21.687190053959608 29.181463089225517 26.704231266095185 22.427115590719694 18-19 21.507637400405656 26.895026801163475 30.124846354669295 21.472489443761578 20-21 22.475615145107028 25.974403732944147 30.244060186274723 21.3059209356741 22-23 21.960692423393837 25.939837912625602 30.173857569800035 21.925612094180526 24-25 21.694941311163763 26.213759901825767 30.221728431348975 21.86957035566149 26-27 21.63074432889849 26.468662063640952 30.128270682293024 21.772322925167533 28-29 21.44042552254883 26.451415411715345 30.35616993135171 21.751989134384115 30-31 21.449241108796528 26.10721860888457 30.4125345042895 22.031005778029403 32-33 21.355357606626107 26.25965576660853 30.170146010191356 22.21484061657401 34-35 21.881801842313063 26.335378993405218 30.00595277599819 21.776866388283526 36-37 21.94826698812152 26.10671669082093 29.915685823028266 22.029330498029285 38-39 21.770819753730194 26.298825198551434 29.98017448484982 21.950180562868553 40-41 21.800198426807256 26.152179139450705 29.89184723167515 22.15577520206689 42-43 21.872077376344873 26.311587150986675 29.57883067287812 22.237504799790333 44-45 21.97005122842922 26.51021267129051 29.390808680491244 22.128927419789022 46-47 22.15945587297653 26.794775031566587 28.706919524420467 22.338849571036416 48-49 22.28479656735021 26.90473360699451 28.276290113355053 22.534179712300226 50-51 22.259345766690775 27.017535539404573 27.992330636296348 22.730788057608304 52-53 22.528855973409975 27.096002828034383 27.913532307042182 22.46160889151346 54-55 22.136780481982292 26.93859724081617 28.06945976043307 22.855162516768466 56-57 22.15831596284001 27.28284851710841 27.89257653513851 22.666258984913064 58-59 22.25196306492082 26.816127098930348 28.03981322525048 22.892096610898356 60-61 22.34524885805322 26.883678880164158 28.237254045488253 22.53381821629437 62-63 22.26864165592996 26.8778792285826 28.254118746539845 22.59936036894759 64-65 22.072638473676523 26.698395046794616 28.179606214344116 23.049360265184742 66-67 21.956024596412178 27.07671980078939 28.036555824708216 22.930699778090222 68-69 22.13080032330823 26.862146017616713 28.106449267731243 22.90060439134382 70-71 22.003015735257318 26.937396573249757 28.187846512416964 22.871741179075958 72-73 22.25583349711629 26.923003937568197 27.86124307875814 22.95991948655737 74-75 21.974220441804672 27.102127753236836 27.79847720119264 23.125174603765856 76-77 22.14307699965563 26.896124604287998 27.976707466186916 22.984090929869456 78-79 22.264074787418767 26.974858662939216 27.89304163856179 22.86802491108023 80-81 21.929466945587993 27.112107817675383 27.930519334083233 23.027905902653387 82-83 22.15482081817366 27.158977206737582 27.71324135665877 22.972960618429987 84-85 21.938895922478295 26.949072711813844 27.649884586303607 23.46214677940425 86-87 22.01037490519016 26.88674964784917 27.84727489435475 23.255600552605916 88-89 22.07776332662094 26.831994522631565 27.754201059648665 23.336041091098835 90-91 21.945636623748214 27.051493680744258 27.80651988047168 23.19634981503585 92-93 21.95583788487781 27.037968854229806 27.847968820368347 23.158224440524037 94-95 21.869868786543893 26.862474929117507 27.779773778777546 23.487882505561057 96-97 22.080131870200766 26.84711908105324 27.67208665020772 23.400662398538266 98-99 21.64098344558103 26.733236492183295 27.64767428867466 23.978105773561012 100-101 21.712303580002697 26.81023337773657 27.808324286765917 23.669138755494817 102-103 21.875021163225693 26.63237885492389 27.76175016151774 23.73084982033268 104-105 21.753120765304498 26.54941180693208 27.901703934808797 23.79576349295462 106-107 21.391140992683408 26.717983481462014 27.985892334238994 23.904983191615585 108-109 21.714765566537658 26.712039792908925 27.492288261565918 24.080906378987503 110-111 21.523748832654654 26.650045610273587 27.784681584758925 24.041523972312838 112-113 21.65756325071014 26.68264208659707 27.694192945013473 23.96560171767932 114-115 21.530644412532645 26.77081982433763 27.572166395386283 24.126369367743447 116-117 21.333495863934797 26.748609178238507 27.703578043470163 24.21431691435653 118-119 21.26926380951833 26.788262912546802 27.548166084318034 24.39430719361684 120-121 21.291656056821815 26.727327890257186 27.706560134794696 24.27445591812631 122-123 21.316417412139007 26.747273279510566 27.53497560164604 24.401333706704392 124-125 21.158817525673353 26.592465718633747 27.515304453479132 24.733412302213768 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 415.0 1 349.0 2 622.0 3 927.5 4 623.5 5 398.5 6 690.0 7 1513.5 8 2353.0 9 2679.0 10 2201.5 11 1420.5 12 1183.0 13 1219.0 14 1327.0 15 1467.0 16 1675.5 17 1948.5 18 2154.0 19 2442.5 20 2818.0 21 3213.5 22 3735.0 23 4490.5 24 5440.0 25 6740.0 26 8777.5 27 11274.0 28 13512.5 29 15900.0 30 18753.5 31 21780.0 32 24590.0 33 27359.0 34 30628.5 35 33257.5 36 36015.0 37 39261.0 38 40910.0 39 41614.0 40 42457.0 41 43786.5 42 45501.0 43 47434.5 44 49663.0 45 51279.0 46 51496.5 47 51781.5 48 54300.5 49 55747.0 50 54326.5 51 52065.5 52 50220.5 53 49490.0 54 47831.5 55 44385.5 56 40513.0 57 37094.0 58 34947.5 59 31898.0 60 27092.0 61 21903.5 62 17295.5 63 13360.5 64 10016.5 65 7775.5 66 5988.0 67 4607.5 68 3483.5 69 2555.5 70 1947.5 71 1622.5 72 1366.0 73 1133.0 74 870.0 75 660.0 76 489.0 77 289.5 78 169.5 79 115.5 80 81.0 81 48.5 82 29.5 83 21.0 84 10.0 85 8.0 86 6.5 87 2.5 88 2.0 89 2.0 90 1.0 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10300376867832146 2 4.0632650366201757E-4 3 0.0 4 0.17810645077185105 5 0.0 6 2.0316325183100878E-4 7 0.038330133512117 8 0.0 9 0.0 10-11 8.803740912677048E-4 12-13 8.4651354929587E-4 14-15 6.094897554930264E-4 16-17 0.005248384005634394 18-19 0.0020316325183100882 20-21 0.00423256774647935 22-23 0.0023025168540847666 24-25 0.00585787376112742 26-27 0.005417686715493569 28-29 0.004097125578592011 30-31 0.0011512584270423833 32-33 1.0158162591550439E-4 34-35 0.0015575849307044008 36-37 0.00169302709859174 38-39 8.4651354929587E-4 40-41 0.0020316325183100882 42-43 0.0012189795109860528 44-45 0.0015914454726762355 46-47 3.047448777465132E-4 48-49 1.0158162591550439E-4 50-51 0.043510796433807716 52-53 6.77210839436696E-5 54-55 0.1456680515628333 56-57 2.0316325183100878E-4 58-59 0.0 60-61 0.0 62-63 8.4651354929587E-4 64-65 0.06734861798197941 66-67 0.055531288833809075 68-69 0.0024040984800002708 70-71 0.0038262412428173324 72-73 0.0755090085971916 74-75 0.0059255948450710905 76-77 0.0023363773960566013 78-79 0.0022347957701410967 80-81 2.708843357746784E-4 82-83 1.6930270985917402E-4 84-85 3.38605419718348E-5 86-87 3.3860541971834804E-4 88-89 0.0015914454726762355 90-91 5.079081295775221E-4 92-93 0.0027088433577467843 94-95 0.005519268341409072 96-97 0.0028104249836622885 98-99 0.0 100-101 0.0017607481825354097 102-103 0.0018284692664790794 104-105 2.708843357746784E-4 106-107 6.77210839436696E-5 108-109 0.0 110-111 2.708843357746784E-4 112-113 1.0158162591550439E-4 114-115 3.7246596169018283E-4 116-117 0.0 118-119 6.433502974648612E-4 120-121 0.0019639114343664187 122-123 5.756292135211916E-4 124-125 0.007720203569578335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1476645.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.75752832955085 #Duplication Level Percentage of deduplicated Percentage of total 1 82.73982503499879 52.75286738651016 2 10.12686381038484 12.913276125602295 3 3.059968320397265 5.852880506257704 4 1.2910153630361905 3.292477943306612 5 0.7031526707684991 2.2415638163260967 6 0.42058390109719085 1.6089233993494294 7 0.28537205497873075 1.2736231815838 8 0.21371023250303214 1.0900508962501578 9 0.15324225257127885 0.8793312533637749 >10 0.9070907792322802 10.68964402395635 >50 0.07014928021294986 3.038882489808979 >100 0.027939206675172617 3.0142336215882195 >500 5.435465719320971E-4 0.2384276793937296 >1k 3.2612794315925827E-4 0.39784153480232787 >5k 2.1741862877283885E-4 0.7159761419003566 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5310 0.35959895574088563 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5020 0.3399598413972214 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3210 0.21738467945917944 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.77210839436696E-5 0.0 0.0 0.0 0.0 8 2.031632518310088E-4 0.0 0.0 0.0 0.0 9 2.031632518310088E-4 0.0 0.0 0.0 0.0 10-11 2.031632518310088E-4 0.0 0.0 6.77210839436696E-5 0.0 12-13 3.7246596169018283E-4 0.0 0.0 2.031632518310088E-4 1.015816259155044E-4 14-15 5.417686715493568E-4 0.0 0.0 2.370237938028436E-4 1.6930270985917402E-4 16-17 5.417686715493568E-4 0.0 0.0 2.708843357746784E-4 3.047448777465132E-4 18-19 6.094897554930264E-4 0.0 0.0 3.047448777465132E-4 3.3860541971834804E-4 20-21 6.772108394366961E-4 0.0 0.0 4.401870456338524E-4 3.3860541971834804E-4 22-23 7.449319233803657E-4 0.0 0.0 5.756292135211916E-4 3.3860541971834804E-4 24-25 8.126530073240352E-4 0.0 0.0 7.449319233803657E-4 3.3860541971834804E-4 26-27 8.126530073240352E-4 0.0 0.0 8.803740912677048E-4 3.3860541971834804E-4 28-29 9.819557171832092E-4 0.0 0.0 0.0016591665566199052 3.3860541971834804E-4 30-31 0.0010835373430987136 0.0 0.0 0.004401870456338525 3.3860541971834804E-4 32-33 0.0011173978850705485 0.0 0.0 0.009243927958310902 3.3860541971834804E-4 34-35 0.001523724388732566 0.0 0.0 0.01628692068845254 3.3860541971834804E-4 36-37 0.00169302709859174 0.0 0.0 0.028375134172397565 3.3860541971834804E-4 38-39 0.0019639114343664183 0.0 0.0 0.051298721087329725 3.3860541971834804E-4 40-41 0.0019639114343664183 0.0 0.0 0.07940297092395261 3.3860541971834804E-4 42-43 0.002167074686197427 0.0 0.0 0.10638982287550494 3.3860541971834804E-4 44-45 0.002573401189859445 0.0 0.0 0.13547602842931106 3.3860541971834804E-4 46-47 0.0032167514873243062 0.0 0.0 0.17160522671325878 4.063265036620176E-4 48-49 0.00345377528112715 0.0 0.0 0.21440495176565794 5.417686715493568E-4 50-51 0.00345377528112715 0.0 0.0 0.2663808836924244 5.417686715493568E-4 52-53 0.0035553569070426545 0.0 0.0 0.3151739246738383 5.417686715493568E-4 54-55 0.003758520158873663 0.0 0.0 0.3653552478760975 5.417686715493568E-4 56-57 0.003860101784789167 0.0 0.0 0.43087539659159785 5.417686715493568E-4 58-59 0.003860101784789167 0.0 0.0 0.5056733338073809 5.417686715493568E-4 60-61 0.003860101784789167 0.0 0.0 0.5896474778975311 5.417686715493568E-4 62-63 0.003860101784789167 0.0 0.0 0.6728428295223293 6.772108394366961E-4 64-65 0.003893962326761002 0.0 0.0 0.7560043206051557 6.772108394366961E-4 66-67 0.004266428288451185 0.0 0.0 0.8554865929184063 6.772108394366961E-4 68-69 0.004368009914366689 0.0 0.0 0.9748450033691239 6.772108394366961E-4 70-71 0.004469591540282194 0.0 0.0 1.1092036339133644 7.449319233803657E-4 72-73 0.004605033708169533 0.0 0.0 1.2484381825015491 7.787924653522005E-4 74-75 0.004706615334085037 0.0 0.0 1.3879774759674803 8.803740912677048E-4 76-77 0.004740475876056872 0.0 0.0 1.538487585032286 9.142346332395397E-4 78-79 0.004740475876056872 0.0 0.0 1.717237386101602 9.480951752113744E-4 80-81 0.006162618638873934 0.0 0.0 1.9255474403123296 9.480951752113744E-4 82-83 0.006230339722817603 0.0 0.0 2.1454716604193966 9.480951752113744E-4 84-85 0.006501224058592282 0.0 0.0 2.3599104727270266 9.480951752113744E-4 86-87 0.00677210839436696 0.0 0.0 2.581087532887051 9.480951752113744E-4 88-89 0.00677210839436696 0.0 0.0 2.83924030488032 9.480951752113744E-4 90-91 0.007313877065916317 0.0 0.0 3.1333191119057053 9.480951752113744E-4 92-93 0.007652482485634665 0.0 0.0 3.456822729904615 0.0010158162591550441 94-95 0.007652482485634665 0.0 0.0 3.7785317391790176 0.0010158162591550441 96-97 0.007720203569578334 0.0 0.0 4.098005952683279 0.0010496768011268788 98-99 0.008194251157184022 0.0 0.0 4.452559687670361 0.0010835373430987136 100-101 0.00860057766084604 0.0 0.0 4.860748521140829 0.0010835373430987136 102-103 0.008973043622536222 0.0 0.0 5.30533743723102 0.0010835373430987136 104-105 0.01015816259155044 0.0 0.0 5.750840587954451 0.0011512584270423833 106-107 0.010429046927325119 0.0 0.0 6.182054589965768 0.0012189795109860528 108-109 0.010462907469296952 0.0 0.0 6.636259899975959 0.0012189795109860528 110-111 0.010835373430987137 0.0 0.0 7.160658113493764 0.0012189795109860528 112-113 0.010970815598874476 0.0 0.0 7.736050303221154 0.0012189795109860528 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3855 0.0 67.185104 1 GTATCAA 6625 0.0 48.912697 1 TATCAAC 8895 0.0 36.19449 2 ATCAACG 8635 0.0 36.041492 3 TCAACGC 8710 0.0 35.5258 4 CAACGCA 8780 0.0 35.241077 5 AACGCAG 8935 0.0 34.096966 6 GTACATG 8000 0.0 32.747807 1 TACATGG 8370 0.0 30.771801 2 CGCAGAG 10690 0.0 28.926851 8 ACATGGG 8315 0.0 28.60917 3 ACGCAGA 10565 0.0 28.55478 7 TGGTATC 1715 0.0 26.768328 2 GCAGAGT 11405 0.0 26.644106 9 GAGTACT 6805 0.0 26.173328 12-13 CATGGGA 5265 0.0 25.365551 4 AACGCCG 215 1.2318742E-7 24.908318 7 CAGAGTA 11445 0.0 24.135715 10-11 AGTACTT 7070 0.0 23.804401 12-13 GTACTTT 7655 0.0 23.694355 14-15 >>END_MODULE