FastQCFastQC Report
Thu 26 May 2016
SRR1364500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711688
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.12636648735049846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41500.054.940121
GGTATCA20600.054.039751
ATCAACG46550.046.9131743
TCAACGC48300.045.892464
CAACGCA50300.044.3033375
AACGCAG49700.044.090466
TATCAAC53900.042.2822842
ACGCAGA56750.037.671017
GTACATG60850.036.0997921
CGCAGAG58000.035.631248
TACATGG64900.032.8236432
ACATGGG64650.030.9256763
GCAGAGT61750.030.3819589
GAGTACT32850.024.62848712-13
AGAGTAC59750.024.60670910-11
CAGAGTA64350.023.58772710-11
CATGGGA37550.023.4853654
GAGTACA46100.022.2109151
TACCGTA1350.002543458222.0553997
GTACTTT39700.021.16563814-15
Produced by FastQC (version 0.11.5)