##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364500_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1711688 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.224205579521502 30.0 25.0 31.0 2.0 34.0 2 25.381618028519217 30.0 25.0 31.0 2.0 34.0 3 25.46378720888386 30.0 25.0 31.0 2.0 34.0 4 28.16533912722412 35.0 26.0 37.0 2.0 37.0 5 28.767741551030326 35.0 28.0 37.0 2.0 37.0 6 28.809366543435488 35.0 28.0 37.0 2.0 37.0 7 28.63424818074322 35.0 27.0 37.0 2.0 37.0 8 28.30306282453344 35.0 27.0 37.0 2.0 37.0 9 28.891170002944463 35.0 25.0 39.0 2.0 39.0 10-11 28.941582519711538 35.0 26.0 39.0 2.0 39.0 12-13 28.769687291141842 35.0 25.5 39.0 2.0 39.0 14-15 29.353096183416604 36.0 25.0 40.0 2.0 41.0 16-17 29.574923993157633 36.0 26.5 40.0 2.0 41.0 18-19 29.532840681245645 36.0 26.0 40.0 2.0 41.0 20-21 29.31913964460813 36.0 25.0 40.0 2.0 41.0 22-23 29.358499621426336 36.0 25.0 40.0 2.0 41.0 24-25 29.378627705516426 36.0 25.0 40.0 2.0 41.0 26-27 29.469457634802602 36.0 25.5 40.0 2.0 41.0 28-29 29.390175370745133 36.0 25.5 40.0 2.0 41.0 30-31 29.03908977570679 35.0 24.5 40.0 2.0 41.0 32-33 29.03704763952309 35.0 25.0 40.0 2.0 41.0 34-35 28.744610291127824 35.0 24.0 40.0 2.0 41.0 36-37 28.510229668023612 35.0 23.5 40.0 2.0 41.0 38-39 28.355682519244162 35.0 23.0 40.0 2.0 41.0 40-41 28.38000704567655 35.0 23.5 40.0 2.0 41.0 42-43 28.240049296367097 35.0 23.0 40.0 2.0 41.0 44-45 27.89627461313043 34.5 20.5 39.0 2.0 41.0 46-47 27.65460557064138 34.0 19.0 39.0 2.0 41.0 48-49 27.41737892653334 34.0 18.0 39.0 2.0 40.5 50-51 27.39878646108403 34.0 18.0 39.0 2.0 40.0 52-53 27.21508242156281 33.5 16.0 39.0 2.0 40.0 54-55 27.101004680759576 33.5 16.0 39.0 2.0 40.0 56-57 26.707315235019465 33.0 13.0 39.0 2.0 40.0 58-59 26.49995355461977 33.0 12.5 38.0 2.0 40.0 60-61 26.108338084978104 33.0 8.5 38.0 2.0 40.0 62-63 26.073525373783074 33.0 9.0 38.0 2.0 40.0 64-65 25.907670089408818 32.5 8.0 38.0 2.0 40.0 66-67 25.672885479129377 32.0 8.0 37.0 2.0 40.0 68-69 25.508152186613447 32.0 7.5 37.0 2.0 40.0 70-71 25.370202980917085 32.0 7.0 36.5 2.0 39.0 72-73 25.346917487299088 32.0 6.5 36.0 2.0 39.0 74-75 25.07453636410374 32.0 2.0 36.0 2.0 39.0 76-77 24.788620356046195 32.0 2.0 35.5 2.0 39.0 78-79 24.486398514215207 32.0 2.0 35.0 2.0 37.5 80-81 24.470586637284363 32.0 2.0 35.0 2.0 37.0 82-83 24.2749733012091 32.0 2.0 35.0 2.0 36.5 84-85 24.086047515668742 32.0 2.0 35.0 2.0 36.0 86-87 23.87865049004258 32.0 2.0 35.0 2.0 36.0 88-89 23.651260334827377 31.0 2.0 35.0 2.0 35.5 90-91 23.4857710634181 31.0 2.0 35.0 2.0 35.0 92-93 23.379144446885178 31.0 2.0 35.0 2.0 35.0 94-95 23.307212237276886 31.0 2.0 35.0 2.0 35.0 96-97 23.170224947537168 31.0 2.0 35.0 2.0 35.0 98-99 23.084445296105365 31.0 2.0 35.0 2.0 35.0 100-101 23.026432971429372 31.0 2.0 35.0 2.0 35.0 102-103 21.951158154990864 29.5 2.0 33.5 2.0 34.5 104-105 22.6836155304004 30.5 2.0 34.0 2.0 35.0 106-107 22.762858651810376 31.0 2.0 35.0 2.0 35.0 108-109 22.710655504975207 31.0 2.0 35.0 2.0 35.0 110-111 22.609426484265825 31.0 2.0 35.0 2.0 35.0 112-113 22.587674272414134 31.0 2.0 35.0 2.0 35.0 114-115 22.545585118315955 31.0 2.0 35.0 2.0 35.0 116-117 22.353106407242443 31.0 2.0 35.0 2.0 35.0 118-119 22.20946691219428 31.0 2.0 35.0 2.0 35.0 120-121 22.063550132968157 31.0 2.0 35.0 2.0 35.0 122-123 21.80540086744781 31.0 2.0 35.0 2.0 35.0 124-125 20.883352281490552 29.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.8843654877999825 1101 2 1.4507623627158175 1101 3 1.8830219189474917 1101 4 2.2879335483082386 1101 5 1.6909264826429649 1101 6 1.7248896605515291 1101 7 1.550336006437579 1101 8 1.911034021611087 1101 9 2.507214002803426 1101 10-11 2.5218502831931673 1101 12-13 2.301417107120564 1101 14-15 2.5962167049189375 1101 16-17 2.295289158573212 1101 18-19 2.2498717689923247 1101 20-21 2.701183956920982 1101 22-23 2.465064712163592 1101 24-25 2.2733203232545627 1101 26-27 2.002982835996022 1101 28-29 1.9842550216511405 1101 30-31 2.111017501955036 1101 32-33 2.408158109895357 1101 34-35 2.660984998816751 1101 36-37 2.6048856506173728 1101 38-39 2.6391360014806367 1101 40-41 2.433908371957042 1101 42-43 2.410285262011346 1101 44-45 2.631740248753246 1101 46-47 2.643319246165966 1101 48-49 2.630658446850969 1101 50-51 2.5683006658947463 1101 52-53 2.483459271870082 1101 54-55 2.5805174314412156 1101 56-57 2.715472085747596 1101 58-59 2.778017222363637 1101 60-61 3.0080726178223927 1101 62-63 2.620153010463458 1101 64-65 2.6000591264203834 1101 66-67 2.723969102190324 1101 68-69 2.5097044302683855 1101 70-71 2.5150528755945487 1101 72-73 2.5233524941451257 1101 74-75 2.434333109529497 1101 76-77 2.6495333843083593 1101 78-79 2.5442042248797527 1101 80-81 2.435484407344717 1101 82-83 2.4231064131838735 1101 84-85 2.231972709384202 1101 86-87 2.298189987880061 1101 88-89 2.4506912970526713 1101 90-91 2.369366264289585 1101 92-93 2.3874361858872106 1101 94-95 2.364452195116481 1101 96-97 2.3140540398798812 1101 98-99 2.1914357692392272 1101 100-101 2.124026854230152 1101 102-103 2.205269849119624 1101 104-105 2.186198516673997 1101 106-107 2.227484439620678 1101 108-109 2.247741028445674 1101 110-111 2.47495285181499 1101 112-113 2.3582306561472812 1101 114-115 2.4119264072657494 1101 116-117 2.456901781126149 1101 118-119 2.464088344563997 1101 120-121 2.355130463709145 1101 122-123 2.435847317455682 1101 124-125 2.401197247501038 1102 1 1.1236639655714953 1102 2 0.836744948490594 1102 3 0.8943342561649281 1102 4 1.5842473820452625 1102 5 1.3451350517408684 1102 6 1.3326478657636933 1102 7 1.2675237766571605 1102 8 1.3299569243271812 1102 9 1.6387276095929373 1102 10-11 1.3988306035735967 1102 12-13 1.6997166753039537 1102 14-15 2.0781787539701604 1102 16-17 1.5429641356258728 1102 18-19 1.6167074877276946 1102 20-21 1.7834283676278702 1102 22-23 1.697298801317885 1102 24-25 1.4296366842592718 1102 26-27 1.3502758522801521 1102 28-29 1.2142277268787147 1102 30-31 1.3132035603588434 1102 32-33 1.2950537344435666 1102 34-35 1.5792120586208256 1102 36-37 1.5935773941773803 1102 38-39 1.643341242634179 1102 40-41 1.218681274581666 1102 42-43 1.272138210774088 1102 44-45 1.531933649460683 1102 46-47 1.585426734125214 1102 48-49 1.7529142471484178 1102 50-51 1.607162050187327 1102 52-53 1.5668939327835112 1102 54-55 1.4220950374854944 1102 56-57 1.4301024749594866 1102 58-59 1.517138397391136 1102 60-61 1.8758141284333263 1102 62-63 1.7491610199038412 1102 64-65 1.840338116509578 1102 66-67 1.6615142106116565 1102 68-69 1.527451731361797 1102 70-71 1.6859774348248528 1102 72-73 1.5454712225354719 1102 74-75 1.6541607206043878 1102 76-77 1.5181451558878258 1102 78-79 1.46837324842992 1102 80-81 0.9959573778931841 1102 82-83 1.0728622912271675 1102 84-85 1.1201546934835243 1102 86-87 1.285616409000248 1102 88-89 1.1256259940598703 1102 90-91 1.0030604133975345 1102 92-93 1.0697630001551275 1102 94-95 1.3142109297039823 1102 96-97 1.4213812305643394 1102 98-99 1.5359924835323184 1102 100-101 1.3939525517159268 1102 102-103 1.3147030727550941 1102 104-105 1.1928064914418428 1102 106-107 1.4207304293070102 1102 108-109 1.471333086352189 1102 110-111 1.2239699824965413 1102 112-113 1.2253167094712225 1102 114-115 1.2142185799546859 1102 116-117 1.4134691521584557 1102 118-119 1.4780817525605485 1102 120-121 1.4139240536239406 1102 122-123 1.547086847974903 1102 124-125 1.411399191104973 1103 1 1.0866881368688794 1103 2 0.9663545491320953 1103 3 1.0875764032751967 1103 4 1.5598216154567837 1103 5 1.0554004904772647 1103 6 1.0217547458216458 1103 7 1.1575245474737308 1103 8 1.4659677220282958 1103 9 2.1750348510940434 1103 10-11 1.6044512669289333 1103 12-13 1.7827584115305228 1103 14-15 2.010878559670246 1103 16-17 1.851398621417843 1103 18-19 1.5464026760797864 1103 20-21 1.8781929273341653 1103 22-23 1.7274791196779518 1103 24-25 1.8060752865821819 1103 26-27 1.689398241281495 1103 28-29 1.5005214684038286 1103 30-31 1.6637678736033088 1103 32-33 1.7514624900649345 1103 34-35 1.6479330630589288 1103 36-37 2.098641060634076 1103 38-39 2.0265090308793248 1103 40-41 1.9982906401312412 1103 42-43 2.0233055846875203 1103 44-45 2.048484192587157 1103 46-47 2.1710166531552773 1103 48-49 2.2084882839375943 1103 50-51 2.022507654559522 1103 52-53 2.046520794871885 1103 54-55 1.9633488981580491 1103 56-57 2.063729226768377 1103 58-59 2.2544469595389707 1103 60-61 2.318208555415268 1103 62-63 2.2092593652698476 1103 64-65 2.0675638707307975 1103 66-67 2.0039096492012973 1103 68-69 1.8418529215823725 1103 70-71 2.055085087124173 1103 72-73 2.147667180635537 1103 74-75 2.152266663286696 1103 76-77 2.0768310119451705 1103 78-79 2.2142974311399506 1103 80-81 1.8134057157291252 1103 82-83 1.8452526247599224 1103 84-85 1.8705225411143154 1103 86-87 1.827774518724354 1103 88-89 1.8251243648727922 1103 90-91 1.8667111545569028 1103 92-93 1.7369572463585001 1103 94-95 1.5270924429437187 1103 96-97 1.616604917686331 1103 98-99 1.6697796677735752 1103 100-101 1.458928541705859 1103 102-103 1.505798616822652 1103 104-105 1.7147467664082185 1103 106-107 1.457733542921524 1103 108-109 1.7707499443813113 1103 110-111 1.8700828941585428 1103 112-113 1.8670819164639703 1103 114-115 1.7116799433083543 1103 116-117 1.7903812153808012 1103 118-119 1.9138276372763094 1103 120-121 1.7245079208178034 1103 122-123 1.880395342302279 1103 124-125 1.7546282799271218 1104 1 1.3095512910252651 1104 2 1.0569411005027582 1104 3 1.0831903840813197 1104 4 1.5248745775398618 1104 5 1.0691223379755215 1104 6 1.0004345814871733 1104 7 1.0709577065536955 1104 8 1.3100652142326332 1104 9 1.8027011832444657 1104 10-11 1.6741546550493922 1104 12-13 1.1091527523765734 1104 14-15 1.5906278608182696 1104 16-17 1.2205529499953087 1104 18-19 0.6290894424197404 1104 20-21 -3.6498188623456933 1104 22-23 -0.6220326256090019 1104 24-25 0.7851821685589293 1104 26-27 0.9708093169081771 1104 28-29 0.6050946104261534 1104 30-31 1.3865840788976342 1104 32-33 1.348547692160281 1104 34-35 1.4802543277993934 1104 36-37 1.6186566788658432 1104 38-39 1.5120868516449804 1104 40-41 1.7704764661487111 1104 42-43 1.720524301813228 1104 44-45 1.863576704714493 1104 46-47 1.6853222673782255 1104 48-49 1.7877169100982009 1104 50-51 1.7529168629936365 1104 52-53 1.7827143318027474 1104 54-55 1.6556500246616892 1104 56-57 1.806366797696132 1104 58-59 1.7765077455355645 1104 60-61 1.8842779805405812 1104 62-63 1.5805138819118056 1104 64-65 1.7935153587003185 1104 66-67 1.767500134052554 1104 68-69 1.682561149874914 1104 70-71 1.8589209705833767 1104 72-73 1.7531062533887862 1104 74-75 1.6022353864659777 1104 76-77 1.617940525210578 1104 78-79 1.5854423107544555 1104 80-81 1.4138936341322719 1104 82-83 1.3497109505823346 1104 84-85 1.3324256919345068 1104 86-87 1.2300474355535655 1104 88-89 1.3038993089780888 1104 90-91 1.3128084799515278 1104 92-93 1.0233126828752965 1104 94-95 1.16324138518182 1104 96-97 1.2859383649166176 1104 98-99 1.1970951614361525 1104 100-101 0.6487168278975091 1104 102-103 1.142896961908896 1104 104-105 1.228557466982096 1104 106-107 1.3270943657776826 1104 108-109 1.4516747158923664 1104 110-111 1.5006872295967284 1104 112-113 1.3974974475242057 1104 114-115 1.2788018838971915 1104 116-117 1.302925910966536 1104 118-119 1.3936718450781633 1104 120-121 1.470205037431242 1104 122-123 1.1271838518037036 1104 124-125 1.3681788784242208 1105 1 1.397916325369163 1105 2 1.0986172352409085 1105 3 1.1756358166563885 1105 4 1.727748648102196 1105 5 1.2928809307612603 1105 6 1.257472696264724 1105 7 1.3549664743965835 1105 8 1.546371930705444 1105 9 2.0218074790351928 1105 10-11 1.5952705660413464 1105 12-13 1.7464555235925445 1105 14-15 1.9709666951692526 1105 16-17 1.7131502804078416 1105 18-19 1.3920803176509935 1105 20-21 1.556708657727981 1105 22-23 1.54974577792429 1105 24-25 1.4940102937717903 1105 26-27 1.438589401535026 1105 28-29 1.158298224368604 1105 30-31 1.4761625249350772 1105 32-33 1.3265118115751413 1105 34-35 1.3349977634968866 1105 36-37 1.5414727557874492 1105 38-39 1.3992651756192274 1105 40-41 1.577744947960479 1105 42-43 1.8655851018175866 1105 44-45 1.9778591883735572 1105 46-47 2.1921382571118393 1105 48-49 1.9144601251010762 1105 50-51 1.992536246520011 1105 52-53 1.9674726439521244 1105 54-55 1.8788539519360015 1105 56-57 2.2347491470311915 1105 58-59 2.0296438568964312 1105 60-61 2.402920545175494 1105 62-63 2.2020832386006255 1105 64-65 1.8828813521121788 1105 66-67 2.1640427894174756 1105 68-69 1.6829921174164575 1105 70-71 1.7698922857325599 1105 72-73 1.754233975629628 1105 74-75 2.1110809492781186 1105 76-77 2.096161576752305 1105 78-79 1.8461548546549729 1105 80-81 1.7890122234304897 1105 82-83 1.9391815695898629 1105 84-85 1.8731714353892777 1105 86-87 1.8151835169435628 1105 88-89 1.8283224377738314 1105 90-91 1.9539475911244004 1105 92-93 1.8601876879839665 1105 94-95 1.7722483820185282 1105 96-97 1.9830130032907824 1105 98-99 1.8183465544006268 1105 100-101 1.8980755874302915 1105 102-103 1.8747991860201907 1105 104-105 1.9213246096407275 1105 106-107 1.937574635406559 1105 108-109 2.0993074255590614 1105 110-111 2.0197365807273115 1105 112-113 2.0295732983564427 1105 114-115 2.0860446180037755 1105 116-117 2.1620004785004454 1105 118-119 2.155310965787219 1105 120-121 2.13218984492287 1105 122-123 2.269971088570884 1105 124-125 2.0115805181034787 1106 1 -0.4910511449830075 1106 2 -0.1860737059626203 1106 3 -0.1987693659706622 1106 4 -0.5687672876589254 1106 5 -0.9118645408478407 1106 6 -0.506720235265032 1106 7 -0.5666176663437952 1106 8 -0.13949650125998403 1106 9 -0.02286979039280368 1106 10-11 -2.3475647772052852 1106 12-13 -0.7288111124063619 1106 14-15 -0.38393221315934056 1106 16-17 -0.5884332503351857 1106 18-19 -3.295402508685001 1106 20-21 -5.753708432159325 1106 22-23 -6.426405074421094 1106 24-25 -6.377615903208106 1106 26-27 -5.338004524346381 1106 28-29 -2.1188753389057524 1106 30-31 -3.722986500776031 1106 32-33 -4.385977198678969 1106 34-35 -3.7057402661445096 1106 36-37 -5.9343691804672645 1106 38-39 -6.332863301015916 1106 40-41 -2.7427070880595856 1106 42-43 -1.3813541385286463 1106 44-45 -0.6240254795948132 1106 46-47 -0.386452976386753 1106 48-49 -0.20054871986053868 1106 50-51 -0.3631877671730308 1106 52-53 -0.4301597968263664 1106 54-55 -0.3756722703951638 1106 56-57 -0.12225712027188962 1106 58-59 -0.02556712943571071 1106 60-61 0.19175289271952778 1106 62-63 -0.028957386103822813 1106 64-65 -0.19818267914207155 1106 66-67 -0.5585203411113255 1106 68-69 -0.4202288784685777 1106 70-71 -0.20610326912159138 1106 72-73 -0.1469998979151228 1106 74-75 -0.1833244795863962 1106 76-77 -0.2122994642837277 1106 78-79 -0.29522091602631306 1106 80-81 -0.6744983924821604 1106 82-83 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84-85 -4.021574830173169 2216 86-87 -4.252946442477985 2216 88-89 -4.677411539600449 2216 90-91 -4.7363402797868055 2216 92-93 -4.393427778422193 2216 94-95 -7.521500017956967 2216 96-97 -9.356508989225947 2216 98-99 -6.452533732616935 2216 100-101 -5.41971715619356 2216 102-103 -6.343809511202561 2216 104-105 -9.232964476896449 2216 106-107 -5.930638970285624 2216 108-109 -7.773358043805018 2216 110-111 -5.455861638658103 2216 112-113 -5.020754310750174 2216 114-115 -5.363347817682438 2216 116-117 -4.87745717274473 2216 118-119 -5.104593938441663 2216 120-121 -5.9131251393687485 2216 122-123 -8.342880439102409 2216 124-125 -7.2378087240778 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 208417.0 3 28871.0 4 16387.0 5 13088.0 6 13197.0 7 14425.0 8 15620.0 9 16294.0 10 17081.0 11 17292.0 12 17746.0 13 18243.0 14 18154.0 15 15667.0 16 12805.0 17 11538.0 18 10475.0 19 9680.0 20 9742.0 21 10463.0 22 13275.0 23 17758.0 24 24125.0 25 30388.0 26 36152.0 27 41135.0 28 46663.0 29 52686.0 30 58314.0 31 65276.0 32 77337.0 33 92245.0 34 112605.0 35 138728.0 36 176200.0 37 182785.0 38 50678.0 39 153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63203271298589 19.039863103265567 13.833541337456767 23.494562846291775 2 14.884976222633528 20.141711584664897 41.09378807899801 23.87952411370356 3 18.776301761867355 25.720843620244644 29.06707066172895 26.435783956159053 4 11.022430421152741 16.634916589556553 38.587537563780245 33.75511542551046 5 13.213965247636168 38.25472401245123 34.403203413857106 14.128107326055506 6 30.79731461125136 37.418710669869064 17.89186116416311 13.892113554716461 7 27.938893656898443 32.758893668583156 21.14458714717297 18.157625527345427 8 25.392897121130332 36.3310361266712 20.003809203315644 18.272257548882827 9 25.832928242904686 17.029164173802492 20.639337864113973 36.49856971917885 10-11 24.2119253381722 27.496312682210206 27.607326815905363 20.684435163712234 12-13 25.050911677624132 24.87586489964462 28.0771876830403 21.99603573969095 14-15 22.241400062870078 26.197861949930644 25.95663600679184 25.60410198040744 16-17 21.931479247893932 29.7117074789861 26.141126242368145 22.215687030751827 18-19 21.69760299798368 28.207307555995254 28.61214988632801 21.482939559693058 20-21 22.53600395251483 27.40179191202905 28.67606256744388 21.386141568012242 22-23 22.259204450333293 27.4149529679358 28.21780688440148 22.10803569732942 24-25 21.977283225189517 27.582296961192394 28.518472097201176 21.921947716416913 26-27 21.856975254975648 27.818696188686655 28.242263089996356 22.082065466341344 28-29 21.79021461133191 27.713417261510916 28.443034443271063 22.053333683886112 30-31 21.88534002553686 27.440293495695805 28.576102441892836 22.0982640368745 32-33 21.822847969898675 27.61846335417698 28.3523292449399 22.206359430984442 34-35 22.21637558291497 27.556973811957864 28.333852908358942 21.892797696768223 36-37 22.179040272069656 27.25884622204513 28.344484072257842 22.217629433627373 38-39 21.873400518378116 27.62466900225067 28.354120264657972 22.147810214713246 40-41 22.183624975016333 27.450357018043174 28.415749235295284 21.950268771645202 42-43 22.04372780485952 27.4416736176746 28.187912404152783 22.326686173313096 44-45 22.102111920251307 27.677566176990954 28.12303519009271 22.097286712665028 46-47 22.08511176553586 27.80745929312195 27.698438270935938 22.408990670406258 48-49 22.15816867289667 27.71314319235385 27.755094647859014 22.37359348689046 50-51 22.196613897091733 27.86415272053316 27.484186072057522 22.45504731031759 52-53 22.392797376699747 27.840106998116198 27.46074632237799 22.306349302806073 54-55 22.326330104632124 27.74850311646842 27.483760329190215 22.44140644970924 56-57 22.095936431889225 28.10569366948088 27.45385761444305 22.344512284186848 58-59 22.21914479183375 27.71086801358783 27.54673190039994 22.523255294178483 60-61 22.32066542671087 27.687531138913524 27.788958159527667 22.20284527484794 62-63 22.198942111791663 27.77800824178232 27.665009468175423 22.358040178250597 64-65 22.10185900647519 27.573709526403967 27.619779349793237 22.7046521173276 66-67 22.052215554587594 27.982300921150227 27.359280725141055 22.60620279912112 68-69 22.098657773999467 27.75601839882327 27.569546996898957 22.575776830278304 70-71 21.95534416159369 27.505555469541243 27.955426540582025 22.583673828283043 72-73 22.196168997824927 27.56029648290234 27.64127017696783 22.6022643423049 74-75 22.1060512539483 27.857186048461323 27.487806381373332 22.54895631621704 76-77 22.192154600097865 27.673125378868107 27.62241000284836 22.51231001818567 78-79 22.203477631297922 27.642152189391915 27.615242955901877 22.539127223408286 80-81 22.04137863342541 27.909924468686754 27.40385064804558 22.644846249842253 82-83 22.244891281993755 27.966228943481592 27.38323097201189 22.405648802512758 84-85 22.162954047852395 27.793563690485755 27.350941434052878 22.692540827608973 86-87 22.198712726063867 27.6705179330586 27.593747726909562 22.53702161396797 88-89 22.26382827560761 27.609021379078047 27.519512210084795 22.60763813522954 90-91 22.209453076114563 27.793468182527253 27.612320934148403 22.38475780720978 92-93 22.270116915177997 27.680879958846138 27.532278527049286 22.516724598926576 94-95 22.22422813674884 27.632485298886134 27.419994683352762 22.723291881012265 96-97 22.327835311740863 27.607373253581642 27.470458260645014 22.59433317403248 98-99 22.071474935163803 27.506702710471536 27.430050227240727 22.991772127123937 100-101 21.96934443502847 27.489986886772584 27.61448805685573 22.926180621343217 102-103 22.212777293846912 27.3177708177419 27.71772945479784 22.75172243361335 104-105 22.05312586449811 27.384086778886253 27.775500850220652 22.787286506394985 106-107 21.912485365152587 27.43347424851081 27.84783614579463 22.806204240541973 108-109 21.988821906795863 27.503437469601728 27.47407985796174 23.033660765640672 110-111 21.853439890070277 27.554642071137664 27.637866395893056 22.954051642899 112-113 21.967476601551123 27.501864485158173 27.68555242365543 22.845106489635274 114-115 21.781765781050673 27.662259473187454 27.55589908704171 23.000075658720164 116-117 21.62426734533625 27.688445323162636 27.733345252117335 22.953942079383776 118-119 21.831865368902562 27.59512167049688 27.415904494626552 23.157108465974005 120-121 21.631770463298807 27.749288365179677 27.603864796395317 23.0150763751262 122-123 21.739326031757532 27.676277201252162 27.6609114077287 22.9234853592616 124-125 21.828140805445116 27.637457047184455 27.438771504904153 23.095630642466272 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 686.0 1 761.5 2 955.5 3 982.5 4 761.5 5 592.5 6 547.5 7 629.5 8 830.0 9 942.0 10 931.0 11 898.5 12 916.5 13 1008.0 14 1125.0 15 1266.5 16 1383.5 17 1534.0 18 1777.5 19 2003.5 20 2317.0 21 2824.0 22 3557.0 23 4467.5 24 5588.0 25 7344.5 26 9994.0 27 12821.5 28 15186.5 29 17833.0 30 21386.0 31 25354.5 32 29402.5 33 33638.5 34 38539.5 35 42477.0 36 45939.5 37 49887.0 38 51835.0 39 52906.5 40 54053.5 41 55874.5 42 57881.5 43 59809.0 44 62035.0 45 62751.0 46 62287.5 47 62280.0 48 62976.5 49 62923.5 50 61569.0 51 59419.5 52 57052.0 53 55190.5 54 52766.0 55 48604.5 56 44010.5 57 39923.0 58 36226.0 59 31905.5 60 26744.5 61 21783.0 62 17536.0 63 13897.5 64 10876.0 65 8568.5 66 6703.5 67 5267.5 68 4039.5 69 3003.5 70 2303.0 71 1780.0 72 1433.5 73 1176.5 74 943.5 75 728.5 76 520.0 77 357.5 78 239.5 79 185.5 80 149.0 81 96.5 82 62.0 83 48.0 84 44.0 85 36.0 86 25.0 87 20.0 88 18.0 89 17.5 90 15.0 91 7.0 92 6.0 93 6.5 94 5.5 95 4.5 96 3.0 97 2.5 98 1.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10434144540360159 2 0.035462070190361794 3 0.04533536485621211 4 0.05439075345506891 5 0.05918134613317381 6 0.0032132024060459617 7 0.0028626712344773115 8 0.0025705619248367695 9 0.007945373222222742 10-11 0.06397193881127869 12-13 0.00850038091053977 14-15 0.012969653348040065 16-17 0.025705619248367693 18-19 0.023631643149919848 20-21 0.14000799211071177 22-23 0.020768971915442535 24-25 0.018081566266749546 26-27 0.008880123013072476 28-29 0.0061635064334154355 30-31 0.0025997728558008236 32-33 0.007916162291258688 34-35 0.02065212819158632 36-37 0.003943475680147316 38-39 0.011450684937909246 40-41 0.03330046129902178 42-43 0.017847878819037113 44-45 0.11199470931618379 46-47 0.005345600366421918 48-49 0.01086646631862816 50-51 0.012969653348040065 52-53 0.014955996653595748 54-55 0.012589911245507358 56-57 0.008062216946078959 58-59 0.017731035095180897 60-61 0.035374437397469635 62-63 0.009639607218137886 64-65 0.009084599529820856 66-67 0.010895677249592214 68-69 0.009668818149101938 70-71 0.005053491056781376 72-73 0.0012268591004902763 74-75 0.0020155542365197397 76-77 0.010253036768383024 78-79 0.022171096601717136 80-81 0.0046737489542486715 82-83 0.006075873640523273 84-85 0.004118741265931642 86-87 0.005053491056781376 88-89 0.007682474843546254 90-91 0.005666920607026514 92-93 0.0036513663705067746 94-95 0.004849014540032996 96-97 0.0024537182009805526 98-99 0.003914264749183262 100-101 0.0033592570608662322 102-103 6.426404812091924E-4 104-105 0.004294006851715967 106-107 0.0011684372385621679 108-109 0.002132397960375956 110-111 0.0028626712344773115 112-113 0.005228756642565701 114-115 0.0031839914750819076 116-117 0.006075873640523273 118-119 0.0045276942994284 120-121 0.007945373222222742 122-123 0.005491655021242189 124-125 0.05517944859109837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1711688.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.4853620638354 #Duplication Level Percentage of deduplicated Percentage of total 1 87.15786020744567 63.1766905384585 2 7.79970298416772 11.307285895955491 3 2.2084981413964795 4.80251362189294 4 0.911053014024903 2.6415203032377437 5 0.49771946618229707 1.8038687856221334 6 0.3032241342959379 1.3187586696560463 7 0.19660631834441217 0.9975756118462676 8 0.15548507396156264 0.9016313505300874 9 0.10647678791931064 0.6946207671352901 >10 0.6040047288412069 8.040357371937338 >50 0.04220147688637838 2.08353475805366 >100 0.01683438891040054 1.9073849367687463 >500 1.6663881183862924E-4 0.10236846231983796 >1k 1.6663881183862924E-4 0.22188892658589066 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2163 0.12636648735049846 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.84218619281084E-5 0.0 10-11 0.0 0.0 0.0 5.84218619281084E-5 0.0 12-13 0.0 0.0 0.0 5.84218619281084E-5 5.84218619281084E-5 14-15 0.0 0.0 0.0 5.84218619281084E-5 5.84218619281084E-5 16-17 0.0 0.0 0.0 1.4605465482027099E-4 5.84218619281084E-5 18-19 0.0 0.0 0.0 2.0447651674837937E-4 5.84218619281084E-5 20-21 0.0 0.0 0.0 2.336874477124336E-4 5.84218619281084E-5 22-23 0.0 0.0 0.0 3.213202406045962E-4 5.84218619281084E-5 24-25 0.0 0.0 0.0 4.965858263889214E-4 1.168437238562168E-4 26-27 0.0 0.0 0.0 6.718514121732465E-4 1.168437238562168E-4 28-29 0.0 0.0 0.0 0.001197648169526222 1.168437238562168E-4 30-31 0.0 0.0 0.0 0.00327162426797407 1.168437238562168E-4 32-33 0.0 0.0 0.0 0.007682474843546254 1.4605465482027099E-4 34-35 0.0 0.0 0.0 0.013261762657680604 1.7526558578432518E-4 36-37 0.0 0.0 0.0 0.022521627773285785 1.7526558578432518E-4 38-39 0.0 0.0 0.0 0.038587639803515594 1.7526558578432518E-4 40-41 0.0 0.0 0.0 0.059415033580886234 1.7526558578432518E-4 42-43 0.0 0.0 0.0 0.07965820873897579 1.7526558578432518E-4 44-45 0.0 0.0 0.0 0.10366959399142835 1.7526558578432518E-4 46-47 0.0 0.0 0.0 0.134224227779829 1.7526558578432518E-4 48-49 0.0 0.0 0.0 0.17035814938236407 1.7526558578432518E-4 50-51 0.0 0.0 0.0 0.210201859217334 1.7526558578432518E-4 52-53 0.0 0.0 0.0 0.250103990914232 2.0447651674837937E-4 54-55 0.0 2.92109309640542E-5 0.0 0.2917587784689733 2.336874477124336E-4 56-57 0.0 1.168437238562168E-4 0.0 0.3417678922794341 2.336874477124336E-4 58-59 0.0 1.4605465482027099E-4 0.0 0.40261426147755897 2.336874477124336E-4 60-61 0.0 1.7526558578432518E-4 0.0 0.47061730876187713 2.336874477124336E-4 62-63 0.0 1.7526558578432518E-4 0.0 0.5410156523852478 2.336874477124336E-4 64-65 0.0 1.7526558578432518E-4 0.0 0.6137216595547788 2.336874477124336E-4 66-67 0.0 2.336874477124336E-4 0.0 0.6971188674571533 2.336874477124336E-4 68-69 0.0 2.9210930964054197E-4 0.0 0.7930767756740714 2.336874477124336E-4 70-71 0.0 2.9210930964054197E-4 0.0 0.9049838521973631 3.5053117156865035E-4 72-73 0.0 2.9210930964054197E-4 0.0 1.0195491234383836 5.257967573529755E-4 74-75 0.0 2.9210930964054197E-4 0.0 1.1376781282570188 5.257967573529755E-4 76-77 0.0 2.9210930964054197E-4 0.0 1.2707923406602137 5.257967573529755E-4 78-79 0.0 2.9210930964054197E-4 0.0 1.4300211253452733 5.257967573529755E-4 80-81 0.0 2.9210930964054197E-4 0.0 1.6111873191843373 5.550076883170297E-4 82-83 0.0 2.9210930964054197E-4 0.0 1.8022852295511798 5.842186192810839E-4 84-85 0.0 2.9210930964054197E-4 0.0 1.9848535480765186 5.842186192810839E-4 86-87 0.0 2.9210930964054197E-4 0.0 2.175922247512397 5.842186192810839E-4 88-89 0.0 2.9210930964054197E-4 0.0 2.4063380709568567 5.842186192810839E-4 90-91 0.0 2.9210930964054197E-4 0.0 2.6686522310140632 5.842186192810839E-4 92-93 0.0 2.9210930964054197E-4 0.0 2.945367380036549 5.842186192810839E-4 94-95 0.0 2.9210930964054197E-4 0.0 3.2270483873229234 6.426404812091924E-4 96-97 0.0 2.9210930964054197E-4 0.0 3.5101607302265365 6.426404812091924E-4 98-99 0.0 2.9210930964054197E-4 0.0 3.8189202705165894 6.426404812091924E-4 100-101 0.0 2.9210930964054197E-4 0.0 4.180405541196761 6.426404812091924E-4 102-103 0.0 3.5053117156865035E-4 0.0 4.56523034571721 6.426404812091924E-4 104-105 0.0 3.5053117156865035E-4 0.0 4.943015315875323 6.426404812091924E-4 106-107 0.0 4.0895303349675874E-4 0.0 5.31665233383654 6.426404812091924E-4 108-109 0.0 4.0895303349675874E-4 0.0 5.705186926589425 6.426404812091924E-4 110-111 0.0 4.0895303349675874E-4 0.0 6.160789816835779 6.426404812091924E-4 112-113 0.0 4.0895303349675874E-4 0.0 6.65319848009684 6.426404812091924E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4150 0.0 54.94012 1 GGTATCA 2060 0.0 54.03975 1 ATCAACG 4655 0.0 46.913174 3 TCAACGC 4830 0.0 45.89246 4 CAACGCA 5030 0.0 44.303337 5 AACGCAG 4970 0.0 44.09046 6 TATCAAC 5390 0.0 42.282284 2 ACGCAGA 5675 0.0 37.67101 7 GTACATG 6085 0.0 36.099792 1 CGCAGAG 5800 0.0 35.63124 8 TACATGG 6490 0.0 32.823643 2 ACATGGG 6465 0.0 30.925676 3 GCAGAGT 6175 0.0 30.381958 9 GAGTACT 3285 0.0 24.628487 12-13 AGAGTAC 5975 0.0 24.606709 10-11 CAGAGTA 6435 0.0 23.587727 10-11 CATGGGA 3755 0.0 23.485365 4 GAGTACA 4610 0.0 22.210915 1 TACCGTA 135 0.0025434582 22.055399 7 GTACTTT 3970 0.0 21.165638 14-15 >>END_MODULE