FastQCFastQC Report
Thu 26 May 2016
SRR1364500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711688
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42300.2471244759558985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41480.24233388327779362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25150.1469309827491926No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32050.069.071061
GTATCAA57650.048.7496951
ATCAACG72500.036.5838053
CAACGCA72850.036.184025
TCAACGC72900.036.137794
AACGCAG73400.035.1832856
TATCAAC79250.035.048242
GTACATG63300.030.164531
ACGCAGA86600.029.4769177
CGCAGAG88000.029.3280548
TGGTATC15500.028.8409212
GTGGTAT16950.028.514491
TACATGG66800.027.1254332
GAGTACT60250.026.50261912-13
GCAGAGT94950.026.1166389
ACATGGG65750.025.9260963
AGAGTAC89350.024.99291610-11
CAGAGTA93950.024.2439610-11
GTACTTT67200.024.07174314-15
AGTACTT67750.023.56874812-13
Produced by FastQC (version 0.11.5)