##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364500_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1711688 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.80701506349288 31.0 28.0 31.0 24.0 34.0 2 28.989834596024508 31.0 28.0 33.0 25.0 34.0 3 29.11432282051402 31.0 28.0 34.0 25.0 34.0 4 32.52328111197835 35.0 32.0 37.0 26.0 37.0 5 31.624084529423587 35.0 32.0 37.0 19.0 37.0 6 31.669758741078983 35.0 32.0 37.0 19.0 37.0 7 31.121100924934918 35.0 31.0 37.0 17.0 37.0 8 31.238601310519208 35.0 32.0 37.0 17.0 37.0 9 31.810505185524466 37.0 30.0 39.0 10.0 39.0 10-11 32.00151838419151 37.0 30.5 39.0 14.0 39.0 12-13 31.738905104201233 37.0 30.0 39.0 10.5 39.0 14-15 32.681339706768995 38.0 31.0 40.0 10.5 41.0 16-17 32.741046557550206 38.0 31.0 40.0 11.0 41.0 18-19 32.61063610891705 38.0 31.0 40.0 10.0 41.0 20-21 32.49851550048841 38.0 30.5 40.0 10.0 41.0 22-23 32.467586382565045 37.5 30.5 40.0 10.0 41.0 24-25 32.279098468879845 37.0 30.0 40.0 9.5 41.0 26-27 32.369509221306686 37.0 30.0 40.0 9.5 41.0 28-29 32.250618979627134 37.0 30.0 40.0 8.5 41.0 30-31 31.7330591205874 37.0 30.0 40.0 8.0 41.0 32-33 31.661997980940452 37.0 30.0 40.0 4.5 41.0 34-35 31.458401005323402 37.0 30.0 40.0 2.0 41.0 36-37 31.4413602245269 37.0 30.0 40.0 2.0 41.0 38-39 31.21176405980529 36.0 30.0 40.0 2.0 41.0 40-41 30.923372133239234 36.0 28.5 40.0 2.0 41.0 42-43 30.809410067722624 36.0 28.5 40.0 2.0 41.0 44-45 30.732993980211347 36.0 29.0 40.0 2.0 41.0 46-47 30.58546504970532 36.0 28.0 40.0 2.0 41.0 48-49 30.309777249124842 36.0 28.0 40.0 2.0 41.0 50-51 29.96487473184365 36.0 27.0 40.0 2.0 41.0 52-53 29.87956596061899 35.5 27.0 40.0 2.0 41.0 54-55 29.418780174891687 35.0 26.0 40.0 2.0 41.0 56-57 29.4415965409584 35.0 26.0 39.0 2.0 41.0 58-59 29.21430073705021 35.0 26.0 39.0 2.0 41.0 60-61 28.942778123115893 35.0 26.0 39.0 2.0 41.0 62-63 28.676361872023406 34.0 25.5 39.0 2.0 41.0 64-65 28.205293838596752 34.0 23.5 38.5 2.0 40.0 66-67 27.832276384481283 34.0 22.5 38.0 2.0 40.0 68-69 27.64423364538397 34.0 22.5 37.5 2.0 40.0 70-71 27.72137737718556 34.0 24.5 37.0 2.0 40.0 72-73 27.48921766116255 34.0 23.5 37.0 2.0 39.0 74-75 27.124888706353026 34.0 22.5 36.0 2.0 39.0 76-77 27.035296736321108 33.5 23.5 36.0 2.0 39.0 78-79 26.631856097606573 33.0 22.0 35.5 2.0 37.5 80-81 26.39430900958586 33.0 21.5 35.0 2.0 37.0 82-83 26.253139590860016 33.0 22.0 35.0 2.0 37.0 84-85 25.815860425498105 33.0 20.0 35.0 2.0 36.0 86-87 25.686051722042805 33.0 20.0 35.0 2.0 36.0 88-89 25.639005765069335 33.0 20.0 35.0 2.0 36.0 90-91 25.469538841190683 33.0 20.0 35.0 2.0 35.0 92-93 25.48343155995719 33.0 20.0 35.0 2.0 35.0 94-95 25.490732247933035 33.0 20.0 35.0 2.0 35.0 96-97 25.367378576002167 33.0 19.5 35.0 2.0 35.0 98-99 25.230109400778645 33.0 18.5 35.0 2.0 35.0 100-101 25.137340449895074 33.0 18.0 35.0 2.0 35.0 102-103 24.018138819691437 30.5 16.0 34.0 2.0 35.0 104-105 24.953698045438188 32.0 18.0 35.0 2.0 35.0 106-107 25.051260217983653 33.0 18.0 35.0 2.0 35.0 108-109 25.124449958169947 33.0 18.0 35.0 2.0 35.0 110-111 25.060363220399978 33.0 17.5 35.0 2.0 35.0 112-113 25.007703214604533 33.0 16.5 35.0 2.0 35.0 114-115 24.95676899061044 33.0 15.0 35.0 2.0 35.0 116-117 24.875921020653298 33.0 8.5 35.0 2.0 35.0 118-119 24.759599880352027 33.0 4.5 35.0 2.0 35.0 120-121 24.598896819981213 33.0 2.0 35.0 2.0 35.0 122-123 24.310081627025486 33.0 2.0 35.0 2.0 35.0 124-125 23.552007141488403 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9576406862915512 1101 2 0.9573369413009516 1101 3 0.9439096017790725 1101 4 1.0975413946816985 1101 5 1.3631500659917357 1101 6 1.2943814377857166 1101 7 1.696598516611374 1101 8 1.5188022213616854 1101 9 2.2793516867664216 1101 10-11 1.8105550877558017 1101 12-13 1.9718165426762688 1101 14-15 1.9984225821687147 1101 16-17 1.9508245830634934 1101 18-19 1.9538974074695545 1101 20-21 2.2442423847037603 1101 22-23 2.231355366144946 1101 24-25 2.378397959551272 1101 26-27 2.0060894770114004 1101 28-29 2.0716612738728557 1101 30-31 2.532182021645596 1101 32-33 2.4129054804137944 1101 34-35 2.386922299284006 1101 36-37 2.25866161279664 1101 38-39 2.3547528779702773 1101 40-41 2.413039342757763 1101 42-43 2.373492692192915 1101 44-45 2.1387252721344545 1101 46-47 1.9610789536399444 1101 48-49 2.062930817746004 1101 50-51 2.1924806691708483 1101 52-53 2.1379094539696304 1101 54-55 2.542869983941035 1101 56-57 2.2405237406501897 1101 58-59 2.3296068507516807 1101 60-61 2.376509614613262 1101 62-63 2.2743748592701998 1101 64-65 2.403588479147917 1101 66-67 2.5762279897083324 1101 68-69 2.514179985059471 1101 70-71 2.1982787995577553 1101 72-73 2.4857062156052123 1101 74-75 2.481486778681237 1101 76-77 2.171353623609736 1101 78-79 2.3330959836137417 1101 80-81 2.310164777624557 1101 82-83 2.193992450791395 1101 84-85 2.4727784881468295 1101 86-87 2.421264551757467 1101 88-89 2.212872700241416 1101 90-91 2.211602703544557 1101 92-93 2.081641918209897 1101 94-95 2.068947448609716 1101 96-97 1.8844240590195582 1101 98-99 2.029750214339572 1101 100-101 2.106666031294104 1101 102-103 2.0283942975379894 1101 104-105 2.0854558411530526 1101 106-107 2.133943928926776 1101 108-109 2.1257555786968467 1101 110-111 2.0826120042217156 1101 112-113 1.9449462834746853 1101 114-115 2.002005290671157 1101 116-117 2.052405021808923 1101 118-119 1.8965838272410807 1101 120-121 1.8424256410395294 1101 122-123 1.849298981248353 1101 124-125 1.6472800545829145 1102 1 0.805545326684296 1102 2 -1.4074139223111644 1102 3 0.23456719654914693 1102 4 0.8356034672466421 1102 5 0.9362474140633346 1102 6 0.4767686897747083 1102 7 1.4534313211087806 1102 8 1.1526530582517402 1102 9 1.9844374189753928 1102 10-11 1.6701645235826916 1102 12-13 1.8056033817187291 1102 14-15 1.8771542596538993 1102 16-17 1.899344872055707 1102 18-19 1.9573215315341983 1102 20-21 2.1215192650310257 1102 22-23 2.0517761815433815 1102 24-25 2.187266023654381 1102 26-27 1.9281355112767002 1102 28-29 1.7840504016122978 1102 30-31 2.1644866660346835 1102 32-33 1.8412192370381995 1102 34-35 2.188907850798966 1102 36-37 1.546756831124565 1102 38-39 1.32967023870917 1102 40-41 1.7890734152793684 1102 42-43 1.6367814273193666 1102 44-45 1.614902959878716 1102 46-47 1.3310352006699482 1102 48-49 1.49611906302437 1102 50-51 1.6993866814083987 1102 52-53 1.3550291877791203 1102 54-55 1.7733279498929981 1102 56-57 1.412164617037952 1102 58-59 1.173859547125378 1102 60-61 1.1503916258286466 1102 62-63 1.6255073242609974 1102 64-65 1.5510146554214579 1102 66-67 1.8201164274445993 1102 68-69 1.8228302200770727 1102 70-71 1.1903871388516052 1102 72-73 1.3791674592657586 1102 74-75 1.5782358202965838 1102 76-77 1.518179742667968 1102 78-79 1.377258508877624 1102 80-81 1.5270769546503722 1102 82-83 1.1109807835672605 1102 84-85 1.2352182808489687 1102 86-87 1.2397964597080744 1102 88-89 1.363862239520234 1102 90-91 1.0908801498334313 1102 92-93 1.2984350491075851 1102 94-95 1.2372437057347163 1102 96-97 1.309452508370569 1102 98-99 1.0788418966034818 1102 100-101 0.8937026396670973 1102 102-103 0.8325323887951477 1102 104-105 1.0749175852013408 1102 106-107 1.3177033071513797 1102 108-109 1.0675877548952464 1102 110-111 1.0475966823095888 1102 112-113 1.1431409193912394 1102 114-115 1.3897228400826869 1102 116-117 0.9678323868551502 1102 118-119 0.484479270405771 1102 120-121 0.7945579809333907 1102 122-123 1.2180479513797344 1102 124-125 1.2528306999156555 1103 1 0.6974812602863558 1103 2 0.6864035731162978 1103 3 0.5725394873319338 1103 4 0.5918706975260264 1103 5 1.0643767904242871 1103 6 0.9620842066542323 1103 7 1.3496613156760233 1103 8 1.1612742355322858 1103 9 1.787483668967024 1103 10-11 1.5152628069699148 1103 12-13 1.5945686224507263 1103 14-15 1.363971515287595 1103 16-17 1.3970832164471503 1103 18-19 1.4853298141790958 1103 20-21 1.7575959597125745 1103 22-23 1.5665690919116528 1103 24-25 1.8806151019729 1103 26-27 1.3747803291167884 1103 28-29 1.492918468163552 1103 30-31 1.9422544781309767 1103 32-33 1.6967232591761636 1103 34-35 1.847178577251558 1103 36-37 1.7631291745679398 1103 38-39 1.778552134365107 1103 40-41 1.9452712008273707 1103 42-43 1.9003802487821453 1103 44-45 1.6176016015175456 1103 46-47 1.5607854860216896 1103 48-49 1.6496829640743158 1103 50-51 1.8151825651909164 1103 52-53 1.788295137591124 1103 54-55 2.0441522862106716 1103 56-57 1.8316801823321711 1103 58-59 1.9401481764696058 1103 60-61 1.8610772379602984 1103 62-63 1.9156089851356164 1103 64-65 1.891591408591033 1103 66-67 2.149442464474287 1103 68-69 2.247407172148666 1103 70-71 1.9920261855410075 1103 72-73 1.9830156149553169 1103 74-75 1.9395080010420997 1103 76-77 1.8796887963431566 1103 78-79 1.9453182638320428 1103 80-81 1.9361119792160544 1103 82-83 1.8994187772157858 1103 84-85 2.00503677990525 1103 86-87 1.9470211624272054 1103 88-89 1.8711839283465714 1103 90-91 1.8385804164561748 1103 92-93 1.6909136888685303 1103 94-95 1.5263331889898346 1103 96-97 1.5471236820107492 1103 98-99 1.7119032879402205 1103 100-101 1.6529067380745879 1103 102-103 1.6223939709410438 1103 104-105 1.655150542781783 1103 106-107 1.5369920016089509 1103 108-109 1.476201751185922 1103 110-111 1.618255485412405 1103 112-113 1.4685751163763356 1103 114-115 1.3527974492850987 1103 116-117 1.3940014099740914 1103 118-119 1.3746411148145228 1103 120-121 1.3824911965877789 1103 122-123 1.6667598684461424 1103 124-125 1.5069488337530643 1104 1 0.5726719479997442 1104 2 0.45864334324175715 1104 3 0.49183049225282005 1104 4 0.5312447844446524 1104 5 0.851718085782398 1104 6 0.9424422287215464 1104 7 1.288025262178536 1104 8 1.0447702980159725 1104 9 1.6153106227257616 1104 10-11 1.1228307886531326 1104 12-13 1.1227792385435613 1104 14-15 1.1515155382535198 1104 16-17 1.1623842407125835 1104 18-19 0.9852352438351772 1104 20-21 1.292778212773662 1104 22-23 1.3998670006021783 1104 24-25 1.4491000393250957 1104 26-27 1.3150021860317196 1104 28-29 1.3537898202100607 1104 30-31 1.8201938939203188 1104 32-33 1.7633829754382297 1104 34-35 1.6972134862191943 1104 36-37 1.718245591602976 1104 38-39 1.8502484949538953 1104 40-41 2.0176187246448336 1104 42-43 1.7985369342193884 1104 44-45 1.620663232324599 1104 46-47 1.6944036289991296 1104 48-49 1.4583849827408635 1104 50-51 1.8850389743159468 1104 52-53 1.6352470937040273 1104 54-55 1.878377199509977 1104 56-57 1.7926775833292616 1104 58-59 1.9016097523908932 1104 60-61 2.0026734589132147 1104 62-63 1.9906605750743047 1104 64-65 2.1395767105031567 1104 66-67 2.061118673373855 1104 68-69 2.144608468421545 1104 70-71 1.9670267236368453 1104 72-73 2.143455279613992 1104 74-75 2.1395297266616495 1104 76-77 1.942988373130781 1104 78-79 2.124768518216854 1104 80-81 2.017747037236237 1104 82-83 1.9235732941267933 1104 84-85 2.1234560023029516 1104 86-87 2.0795099767599226 1104 88-89 1.9834447111145899 1104 90-91 2.0405199829123575 1104 92-93 1.766787691037056 1104 94-95 1.7227389105628994 1104 96-97 1.7799460485024454 1104 98-99 1.79824816432615 1104 100-101 1.7783937820719942 1104 102-103 1.651463724304815 1104 104-105 1.519623639335009 1104 106-107 1.544536307075198 1104 108-109 1.5323605943874838 1104 110-111 1.5608097860088819 1104 112-113 1.6642918734879437 1104 114-115 1.5106290979300354 1104 116-117 1.4780733022688288 1104 118-119 1.3827236506449765 1104 120-121 1.4522891550521635 1104 122-123 1.540715895254074 1104 124-125 1.3619856126037462 1105 1 0.5975716136882703 1105 2 0.6851889548246817 1105 3 0.44780872794921933 1105 4 0.628049344376862 1105 5 0.8808268882280252 1105 6 1.0326999237433867 1105 7 1.2007115243473905 1105 8 1.1981800298311462 1105 9 1.7138885047658512 1105 10-11 1.422441644103607 1105 12-13 1.3673196351944519 1105 14-15 1.3925877598854086 1105 16-17 1.2917724534403874 1105 18-19 1.580456660476635 1105 20-21 1.9028674306156077 1105 22-23 1.7313067196388587 1105 24-25 1.9217089766396498 1105 26-27 1.5805637448441132 1105 28-29 1.6303553896702923 1105 30-31 1.9717315359754508 1105 32-33 1.9263415638645647 1105 34-35 2.066971035473596 1105 36-37 1.738781429956429 1105 38-39 1.8368487216426885 1105 40-41 2.045510437153318 1105 42-43 1.9682638314438101 1105 44-45 1.9486853181339363 1105 46-47 1.8173714675269643 1105 48-49 1.9398315814553833 1105 50-51 2.0886286676049224 1105 52-53 1.999982137556863 1105 54-55 2.194589943670426 1105 56-57 1.89528852324311 1105 58-59 1.8132937612949682 1105 60-61 1.776944076830894 1105 62-63 1.7280278517844785 1105 64-65 2.120797846508257 1105 66-67 2.303089408177943 1105 68-69 2.3536688679060482 1105 70-71 2.1121919448900073 1105 72-73 2.3376662010003884 1105 74-75 2.4739179666357103 1105 76-77 2.2364624878320356 1105 78-79 2.290512218202956 1105 80-81 2.226136974651606 1105 82-83 2.2059906766886535 1105 84-85 2.446661836814684 1105 86-87 2.3203893630654164 1105 88-89 2.1055553145372663 1105 90-91 2.1524317704570066 1105 92-93 2.071156150234433 1105 94-95 1.9133950138588816 1105 96-97 1.9899888796056722 1105 98-99 2.0429501312328604 1105 100-101 2.1316388103127295 1105 102-103 2.021691135121028 1105 104-105 1.71019023870344 1105 106-107 1.841647864116581 1105 108-109 1.8584994875903647 1105 110-111 1.9954158597426783 1105 112-113 1.992617073815989 1105 114-115 1.943923264780615 1105 116-117 2.0285162581598755 1105 118-119 2.003690524655596 1105 120-121 2.0730698009517354 1105 122-123 2.2636486542364835 1105 124-125 2.080300881674077 1106 1 0.5845236863641396 1106 2 0.6066078646306963 1106 3 0.5490154034468588 1106 4 0.3539365407562727 1106 5 0.5375682264055222 1106 6 0.7514514102621632 1106 7 0.9669044357304415 1106 8 0.8029663824555051 1106 9 1.514341581698826 1106 10-11 1.1520467463677093 1106 12-13 1.0384734899579975 1106 14-15 1.2011962635842863 1106 16-17 1.05230260269947 1106 18-19 1.1201743436283422 1106 20-21 1.054322987831391 1106 22-23 1.0011416370066044 1106 24-25 0.7975012037064104 1106 26-27 0.6969892946671834 1106 28-29 0.8383160789784867 1106 30-31 1.196406152754406 1106 32-33 1.0620733200469452 1106 34-35 1.0860618540061004 1106 36-37 0.9946890304674945 1106 38-39 1.089116834506747 1106 40-41 1.3124424806500414 1106 42-43 1.4164663819043035 1106 44-45 1.3271912639292403 1106 46-47 1.1261817180567917 1106 48-49 1.2494808581608154 1106 50-51 1.5118301407765102 1106 52-53 1.2536572317007177 1106 54-55 1.6272505730421898 1106 56-57 1.4048105679318539 1106 58-59 1.3501277379236747 1106 60-61 1.4719757603172567 1106 62-63 1.5827495670746536 1106 64-65 1.7539853820797795 1106 66-67 1.8773330114602835 1106 68-69 1.652997157288791 1106 70-71 1.5501836853019988 1106 72-73 1.4244169885405498 1106 74-75 1.7174568215083852 1106 76-77 1.3338112098875854 1106 78-79 1.3326910168215633 1106 80-81 1.4386856555183378 1106 82-83 1.2565630948468716 1106 84-85 1.4958109744519135 1106 86-87 1.58868823434646 1106 88-89 1.3647530532938639 1106 90-91 1.4805004488837525 1106 92-93 1.519190321838444 1106 94-95 1.3299615766021482 1106 96-97 1.254374254107674 1106 98-99 1.19598314719968 1106 100-101 0.9514234168896216 1106 102-103 0.8510601851984525 1106 104-105 1.1013297753181135 1106 106-107 1.0434441524225981 1106 108-109 0.976966849654012 1106 110-111 1.0399204131770503 1106 112-113 0.8167360893362279 1106 114-115 0.8735628682711436 1106 116-117 1.05501028997935 1106 118-119 0.8523734060592432 1106 120-121 0.8591613576369994 1106 122-123 1.0022549250092716 1106 124-125 1.0223156665548707 1107 1 0.3617708957814685 1107 2 0.34127247106487246 1107 3 0.15273754312412535 1107 4 0.30840164104139234 1107 5 0.5404992412865717 1107 6 0.5703865448614636 1107 7 0.8094460431486254 1107 8 0.6971829835311354 1107 9 1.4468058687932306 1107 10-11 0.8202129901042596 1107 12-13 1.1733024846873796 1107 14-15 0.9738603844562377 1107 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-3.2008032829035855 2216 96-97 -3.352937009615303 2216 98-99 -3.2587370733352152 2216 100-101 -3.697510180827649 2216 102-103 -3.7053551555182835 2216 104-105 -3.4586523304032184 2216 106-107 -3.497313694460413 2216 108-109 -3.5832087077285877 2216 110-111 -3.2921877263015986 2216 112-113 -3.2798446525508957 2216 114-115 -3.1599647227720453 2216 116-117 -3.1655004224063923 2216 118-119 -3.35984911171413 2216 120-121 -3.330747720082204 2216 122-123 -3.376063980054326 2216 124-125 -3.474528653220723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91912.0 3 29505.0 4 14121.0 5 11270.0 6 11506.0 7 13444.0 8 14806.0 9 15970.0 10 16584.0 11 17790.0 12 19302.0 13 21006.0 14 21488.0 15 17751.0 16 12734.0 17 11312.0 18 9744.0 19 9116.0 20 8829.0 21 9668.0 22 11531.0 23 15826.0 24 21888.0 25 28820.0 26 35581.0 27 40123.0 28 45545.0 29 51484.0 30 57410.0 31 65198.0 32 76442.0 33 90430.0 34 110468.0 35 139456.0 36 194222.0 37 256213.0 38 92769.0 39 424.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.27376987341254 20.44886059588803 14.183526962339574 24.09384256835986 2 15.659355746322632 20.854701154300496 38.88955100409772 24.596392095279157 3 19.062235641074775 25.213531905347235 29.759278560111426 25.964953893466564 4 12.768577435047455 17.645402872005207 36.92000383932877 32.66601585361857 5 12.89499020849594 38.820275657713324 34.28153962638051 14.00319450741023 6 31.183519378296097 37.994827313234985 17.05082074192476 13.770832566544156 7 27.28871459338463 32.94208408459306 20.982224001542942 18.786977320479366 8 25.414795219689566 36.94002645341909 19.221259949243084 18.423918377648263 9 25.3335888316095 16.408480984852382 20.222493818967006 38.03543636457111 10-11 24.764550186499097 27.380561854632653 27.14044554720082 20.714442411667434 12-13 25.418968874010655 24.65167840226445 27.901073365378014 22.02827935834688 14-15 22.37118611886018 25.945871733827975 25.977916368469483 25.705025778842362 16-17 21.78815894330698 29.403588680559288 26.495556215105832 22.3126961610279 18-19 21.61734252873026 27.539160233072874 29.287761804594215 21.555735433602656 20-21 22.301179144349877 26.812921657666923 29.46227921168365 21.42361998629955 22-23 21.943183477930653 26.65010370110712 29.310022492916193 22.096690328046037 24-25 21.821559318389756 26.948183973535777 29.313522949951913 21.916733758122543 26-27 21.64592242519351 27.115338605789496 29.346818575256655 21.89192039376034 28-29 21.49652698053251 26.970571206392997 29.61586751288105 21.917034300193443 30-31 21.587363904682448 26.76900543797045 29.481885505834075 22.161745151513028 32-33 21.57411510467314 26.930151596189837 29.43694972002742 22.058783579109598 34-35 22.065116866014943 26.990128302274808 29.127993218283294 21.81676161342696 36-37 22.01904996709328 26.71724729815825 29.146735925111145 22.116966809637333 38-39 21.680175874817138 27.035641174243665 29.292411162886346 21.99177178805285 40-41 21.87718903634202 26.822110764143474 29.28685979359158 22.01384040592292 42-43 21.94932006203322 26.847216173038685 28.98878780788397 22.21467595704413 44-45 22.023855600965955 27.147350239400215 28.703780247810112 22.12501391182372 46-47 22.056839329911558 27.31297736235854 28.262831146744627 22.367352160985273 48-49 22.21356929533887 27.35346628591192 28.10836437481597 22.32460004393324 50-51 22.153909364725493 27.426117058411148 27.793913561100652 22.626060015762707 52-53 22.35171431701172 27.518019498302117 27.677803337349836 22.452462847336328 54-55 22.18276549776608 27.31554582593032 27.844555196284855 22.657133480018743 56-57 22.065466034422876 27.72513461178379 27.7470428932018 22.46235646059153 58-59 22.155877706684862 27.439989063427443 27.816430330761214 22.587702899126477 60-61 22.29457120690219 27.41819770892826 27.979719434850274 22.307511649319267 62-63 22.104452315894818 27.419550836580157 27.99095153215776 22.485045315367262 64-65 22.016978609547515 27.19795653396453 27.88600995076063 22.89905490572733 66-67 21.91942210293762 27.6173466151923 27.605655867045797 22.85757541482428 68-69 21.94854202000527 27.368724545745067 27.915127458959166 22.767605975290493 70-71 22.011431816462295 27.283726911215734 27.99214665115316 22.712694621168815 72-73 22.153400298049696 27.281310857416134 27.767088601674526 22.79820024285964 74-75 21.999477682311657 27.53838217062144 27.582376043028695 22.879764104038205 76-77 22.11196827397118 27.419143290891938 27.72224184261485 22.74664659252203 78-79 22.17858663944928 27.38037719137838 27.668636889178927 22.77239927999341 80-81 21.887477642664123 27.5432738413255 27.67004939280645 22.89919912320393 82-83 22.08605372537553 27.590800067652577 27.583088375120095 22.7400578318518 84-85 21.944624253952824 27.358607409761593 27.51222769570155 23.184540640584032 86-87 21.991490221875843 27.346410948788265 27.818871306402254 22.843227522933635 88-89 22.098661064301396 27.279777852818725 27.594297458955 23.027263623924874 90-91 21.937382023870104 27.51929243825222 27.763029442986166 22.780296094891515 92-93 22.125431163416778 27.444486249509243 27.687410028230104 22.742672558843875 94-95 22.00622102153754 27.307877467843255 27.634410989380797 23.051490521238406 96-97 22.149508003254176 27.35463931679858 27.547465234518203 22.948387445429038 98-99 21.77239660498876 27.15477353349442 27.59717308294502 23.475656778571796 100-101 21.795391608146478 27.189073764649525 27.718501337882522 23.29703328932148 102-103 22.058261207656805 27.095583305850163 27.664360806670274 23.181794679822758 104-105 21.925960503154503 27.048906893282098 27.76621344748233 23.258919156081067 106-107 21.583116076970825 27.162697630262535 27.814247635739587 23.439938657027056 108-109 21.87983440907455 27.21138431770276 27.4099602263964 23.49882104682629 110-111 21.65964725945166 27.21143459390553 27.607857296006326 23.52106085063648 112-113 21.836676093053363 27.156464874221857 27.55420172350687 23.45265730921791 114-115 21.606604018846927 27.30248264137385 27.448011754499223 23.64290158528 116-117 21.536722813970773 27.307166960333895 27.47358163403611 23.682528591659228 118-119 21.574088787831343 27.272947684024313 27.32397953818363 23.82898398996072 120-121 21.449455712860598 27.35121381947457 27.4966881226658 23.702642344999028 122-123 21.542978928255113 27.345391300690086 27.36820519771265 23.74342457334215 124-125 21.41651259079132 27.239134619907063 27.35911369149048 23.985239097811135 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262.0 1 227.5 2 319.0 3 470.0 4 363.0 5 268.0 6 510.5 7 1092.0 8 1716.5 9 1992.5 10 1613.0 11 1088.0 12 959.5 13 991.5 14 1093.5 15 1239.0 16 1424.0 17 1643.0 18 1873.5 19 2152.5 20 2552.0 21 3011.0 22 3649.5 23 4540.5 24 5631.0 25 7306.5 26 9761.0 27 12515.0 28 14887.5 29 17554.5 30 21103.5 31 25063.5 32 29065.5 33 33220.5 34 37853.5 35 41559.5 36 45614.0 37 49434.0 38 51342.5 39 52682.0 40 53420.0 41 55169.0 42 57352.5 43 59366.0 44 61408.0 45 62779.0 46 62699.0 47 62009.5 48 63113.5 49 63988.0 50 62587.5 51 59777.0 52 57217.5 53 55896.5 54 53997.5 55 49721.5 56 44913.0 57 40743.5 58 37024.0 59 32647.0 60 27385.5 61 22174.0 62 17486.0 63 13563.0 64 10457.5 65 8258.5 66 6424.5 67 5042.5 68 3903.5 69 2806.0 70 2013.5 71 1539.5 72 1257.5 73 1100.0 74 908.5 75 711.0 76 488.5 77 300.0 78 186.5 79 111.0 80 78.0 81 50.0 82 30.5 83 20.5 84 12.5 85 8.5 86 7.5 87 6.0 88 5.0 89 3.5 90 1.0 91 1.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10533461705637943 2 1.1684372385621678E-4 3 0.0 4 0.17853721005229925 5 0.0 6 2.9210930964054197E-4 7 0.03931791307761695 8 0.0 9 0.0 10-11 0.001022382583741897 12-13 9.0553885988568E-4 14-15 7.594842050654091E-4 16-17 0.004907436401961105 18-19 0.0020155542365197397 20-21 0.0038266319562911 22-23 0.002220030753268119 24-25 0.005491655021242189 26-27 0.005170334780637592 28-29 0.0037974210253270456 30-31 0.0011684372385621679 32-33 1.4605465482027099E-4 34-35 0.0015773902720589265 36-37 0.0018402886507354142 38-39 9.347497908497342E-4 40-41 0.001927921443627577 42-43 0.0014313356172386557 44-45 8.471169979575717E-4 46-47 2.0447651674837937E-4 48-49 0.0 50-51 0.04016503007557452 52-53 2.9210930964054195E-5 54-55 0.13793401601226393 56-57 3.797421025327046E-4 58-59 0.0 60-61 0.0 62-63 9.931716527778428E-4 64-65 0.06221928295343544 66-67 0.05453680810988919 68-69 0.0022784526151962275 70-71 0.003592944508578666 72-73 0.07215099948121387 74-75 0.005024280125817322 76-77 0.001957132374591631 78-79 0.0021616088913400107 80-81 8.763279289216259E-5 82-83 8.763279289216259E-5 84-85 0.0 86-87 5.842186192810839E-4 88-89 0.0017526558578432518 90-91 4.0895303349675874E-4 92-93 0.0023368744771243358 94-95 0.004206374058823804 96-97 0.00236608540808839 98-99 0.0 100-101 0.001635812133987035 102-103 0.0020155542365197397 104-105 4.0895303349675874E-4 106-107 2.9210930964054195E-5 108-109 0.0 110-111 2.9210930964054197E-4 112-113 2.0447651674837937E-4 114-115 1.7526558578432518E-4 116-117 0.0 118-119 7.010623431373007E-4 120-121 0.002658194717728932 122-123 7.010623431373007E-4 124-125 0.008149849738971121 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1711688.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.23354595554103 #Duplication Level Percentage of deduplicated Percentage of total 1 83.45164487718567 54.43846711161382 2 9.761849142810616 12.736000693371905 3 2.9535205386778807 5.780058533714336 4 1.219295472148411 3.1815586886310596 5 0.6660812775854839 2.172542181574907 6 0.4225020540154824 1.6536784294157674 7 0.27639324074203847 1.2621077820222677 8 0.18164948092232377 0.9479711809237266 9 0.14289031719890338 0.8389117866236852 >10 0.8284012668742969 10.053132746800333 >50 0.06588614542588521 2.9562109857097436 >100 0.02915514446063035 3.116079554415542 >500 3.6552097611722257E-4 0.1469400636359466 >1k 3.6552097611722257E-4 0.7163402615469251 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4230 0.2471244759558985 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4148 0.24233388327779362 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2515 0.1469309827491926 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.84218619281084E-5 2 0.0 0.0 0.0 0.0 5.84218619281084E-5 3 0.0 0.0 0.0 0.0 5.84218619281084E-5 4 0.0 0.0 0.0 0.0 5.84218619281084E-5 5 5.84218619281084E-5 0.0 0.0 0.0 5.84218619281084E-5 6 5.84218619281084E-5 0.0 0.0 0.0 5.84218619281084E-5 7 5.84218619281084E-5 0.0 0.0 5.84218619281084E-5 5.84218619281084E-5 8 5.84218619281084E-5 0.0 0.0 5.84218619281084E-5 5.84218619281084E-5 9 5.84218619281084E-5 0.0 0.0 5.84218619281084E-5 5.84218619281084E-5 10-11 5.84218619281084E-5 0.0 0.0 8.76327928921626E-5 5.84218619281084E-5 12-13 5.84218619281084E-5 0.0 0.0 1.168437238562168E-4 5.84218619281084E-5 14-15 5.84218619281084E-5 0.0 0.0 1.7526558578432518E-4 5.84218619281084E-5 16-17 5.84218619281084E-5 0.0 0.0 2.6289837867648775E-4 5.84218619281084E-5 18-19 5.84218619281084E-5 0.0 0.0 3.797421025327045E-4 5.84218619281084E-5 20-21 5.84218619281084E-5 0.0 0.0 4.3816396446081296E-4 5.84218619281084E-5 22-23 5.84218619281084E-5 0.0 0.0 5.550076883170297E-4 5.84218619281084E-5 24-25 5.84218619281084E-5 0.0 0.0 6.426404812091924E-4 5.84218619281084E-5 26-27 5.84218619281084E-5 0.0 0.0 7.886951360294634E-4 5.84218619281084E-5 28-29 5.84218619281084E-5 0.0 0.0 0.001197648169526222 5.84218619281084E-5 30-31 5.84218619281084E-5 0.0 0.0 0.0033884679918302868 5.84218619281084E-5 32-33 5.84218619281084E-5 0.0 0.0 0.007886951360294634 5.84218619281084E-5 34-35 5.84218619281084E-5 0.0 0.0 0.013816770345997636 5.84218619281084E-5 36-37 5.84218619281084E-5 0.0 0.0 0.023836119666668223 5.84218619281084E-5 38-39 5.84218619281084E-5 0.0 0.0 0.04001897542075425 5.84218619281084E-5 40-41 5.84218619281084E-5 0.0 0.0 0.06037899430270002 5.84218619281084E-5 42-43 5.84218619281084E-5 0.0 0.0 0.08094348970139417 1.168437238562168E-4 44-45 5.84218619281084E-5 0.0 0.0 0.10539303891830754 1.7526558578432518E-4 46-47 5.84218619281084E-5 0.0 0.0 0.13673636784273768 2.336874477124336E-4 48-49 5.84218619281084E-5 0.0 0.0 0.17307476596202112 3.5053117156865035E-4 50-51 5.84218619281084E-5 0.0 0.0 0.21221741345385375 3.5053117156865035E-4 52-53 5.84218619281084E-5 0.0 0.0 0.2526453419081047 3.797421025327045E-4 54-55 5.84218619281084E-5 0.0 0.0 0.29538093390851605 4.0895303349675874E-4 56-57 5.84218619281084E-5 0.0 0.0 0.34644164123368276 4.0895303349675874E-4 58-59 5.84218619281084E-5 0.0 0.0 0.40798907277494495 4.0895303349675874E-4 60-61 5.84218619281084E-5 0.0 0.0 0.47584606540444285 4.0895303349675874E-4 62-63 5.84218619281084E-5 0.0 2.92109309640542E-5 0.5430019956908034 4.673748954248672E-4 64-65 5.84218619281084E-5 0.0 5.84218619281084E-5 0.6127869097639289 5.257967573529755E-4 66-67 5.84218619281084E-5 0.0 5.84218619281084E-5 0.6953370006683461 5.257967573529755E-4 68-69 5.84218619281084E-5 0.0 5.84218619281084E-5 0.7890164562700679 5.257967573529755E-4 70-71 5.84218619281084E-5 0.0 5.84218619281084E-5 0.8989079785568398 5.257967573529755E-4 72-73 5.84218619281084E-5 0.0 5.84218619281084E-5 1.0081860712933666 7.010623431373007E-4 74-75 5.84218619281084E-5 0.0 5.84218619281084E-5 1.1206189445740113 7.010623431373007E-4 76-77 5.84218619281084E-5 0.0 5.84218619281084E-5 1.246722533545833 7.010623431373007E-4 78-79 5.84218619281084E-5 0.0 5.84218619281084E-5 1.398590163627951 7.010623431373007E-4 80-81 5.84218619281084E-5 0.0 5.84218619281084E-5 1.5720446716925047 7.010623431373007E-4 82-83 5.84218619281084E-5 0.0 5.84218619281084E-5 1.7564824897995428 7.010623431373007E-4 84-85 5.84218619281084E-5 0.0 5.84218619281084E-5 1.933354676786891 7.010623431373007E-4 86-87 5.84218619281084E-5 0.0 5.84218619281084E-5 2.1182890807203183 7.010623431373007E-4 88-89 5.84218619281084E-5 0.0 5.84218619281084E-5 2.3422200774907576 7.010623431373007E-4 90-91 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 2.6003570744201046 7.010623431373007E-4 92-93 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 2.8743848177938967 7.010623431373007E-4 94-95 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 3.149785474923 7.010623431373007E-4 96-97 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 3.428895920284538 7.010623431373007E-4 98-99 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 3.7391452180537574 7.010623431373007E-4 100-101 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 4.103347105313585 7.010623431373007E-4 102-103 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 4.494101728819738 7.594842050654091E-4 104-105 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 4.875567276279321 7.594842050654091E-4 106-107 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 5.256507026981552 7.594842050654091E-4 108-109 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 5.649423259379046 7.594842050654091E-4 110-111 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 6.109203312753259 7.886951360294634E-4 112-113 5.84218619281084E-5 5.84218619281084E-5 5.84218619281084E-5 6.605584662625432 8.179060669935175E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3205 0.0 69.07106 1 GTATCAA 5765 0.0 48.749695 1 ATCAACG 7250 0.0 36.583805 3 CAACGCA 7285 0.0 36.18402 5 TCAACGC 7290 0.0 36.13779 4 AACGCAG 7340 0.0 35.183285 6 TATCAAC 7925 0.0 35.04824 2 GTACATG 6330 0.0 30.16453 1 ACGCAGA 8660 0.0 29.476917 7 CGCAGAG 8800 0.0 29.328054 8 TGGTATC 1550 0.0 28.840921 2 GTGGTAT 1695 0.0 28.51449 1 TACATGG 6680 0.0 27.125433 2 GAGTACT 6025 0.0 26.502619 12-13 GCAGAGT 9495 0.0 26.116638 9 ACATGGG 6575 0.0 25.926096 3 AGAGTAC 8935 0.0 24.992916 10-11 CAGAGTA 9395 0.0 24.24396 10-11 GTACTTT 6720 0.0 24.071743 14-15 AGTACTT 6775 0.0 23.568748 12-13 >>END_MODULE